CN114525169B - Method for continuously grading peony seeds - Google Patents
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- CN114525169B CN114525169B CN202210058277.4A CN202210058277A CN114525169B CN 114525169 B CN114525169 B CN 114525169B CN 202210058277 A CN202210058277 A CN 202210058277A CN 114525169 B CN114525169 B CN 114525169B
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a method for continuously grading peony seeds, and belongs to the technical field of food processing. According to the invention, the peony seeds are treated by a wet continuous grading treatment method, the whole process of peony seed oil production flow is realized, and functional components such as phytosterol, vitamin E, squalene and the like in the peony seeds can be effectively extracted. Meanwhile, the method improves the yield of the peony seed oil and effectively reduces the peculiar smell and the variegated color in the crude oil. The carbohydrate slag obtained by the method has no bitter taste and black color, and has good palatability when used in the fields of food and feed. The invention also realizes the automatic precipitation of the peony seed protein and improves the efficiency of continuous production by adjusting the volume ratio of the mixture of liquid phase products with different ethanol concentrations in the continuous production. In the invention, the solvents such as alcohol and ether can be recycled through desolventizing and separating, thereby reducing the production cost.
Description
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a method for continuously treating peony seeds in a grading manner.
Background
The peony seeds are seeds formed after the peony blooms, the peony seeds are coated with black tough shells, the majority of the shapes are round, the minority of the shapes are oval, the seed kernels are milky white, and the taste is slightly bitter. Wherein, the oil content of the peony seeds for oil reaches 24.12-37.83 percent, and the content of unsaturated fatty acid reaches 85-92 percent. After oil is pressed from peony seeds, at least 70% of cake pulp is generated, and the peony seed cake pulp contains 5% -7% of fat, 20% -25% of protein and about 50% of carbohydrate. The prior art mainly comprises the steps of manually or mechanically shelling, manually screening the peony seed shells for the second time, separating the shells and kernels, and then carrying out conventional oil extraction, protein extraction and functional substance extraction processes. However, the peony seeds have tight combination of shells and kernels, hard shells, flat and small seed particles and high oil and glue content, so that the shelling effect is poor, the process is complex and the energy consumption is high. Resulting in incomplete utilization of the peony seed components. In addition, the existing process is discontinuous in treatment process, needs to consume a large amount of manpower and energy, and cannot realize large-scale production. Meanwhile, the backward extraction technology also causes the quality and efficacy of the finished oil to be difficult to ensure. Therefore, the development of a method capable of continuously treating peony seeds in a grading manner is a problem to be solved.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for continuously grading peony seeds.
In order to realize the purpose, the invention adopts the technical scheme that: the invention provides a method for continuously grading peony seeds, which comprises the following steps:
(1) Washing peony seeds with water, and draining; the peony seeds are fresh peony seeds which are not dried;
(2) Crushing and squeezing the peony seeds obtained in the step (1) to obtain a mixture, adding a solvent into the mixture, performing fusion extraction to obtain a feed liquid, and performing solid-liquid separation on the feed liquid to obtain a solid phase I and a liquid phase I respectively; the fusion extraction is that the solvent and the mixture are mixed and leached evenly;
(3) Standing and layering the liquid phase I obtained in the step (2), wherein the separated lower layer is an alcohol solution I, and the upper layer is an oil phase I;
(4) Uniformly mixing the solid phase I obtained in the step (2) with the leaching liquor, standing for layering, separating out an ether phase I as the upper layer of liquid, an alcohol solution II as the lower layer of the liquid, and taking the residual solid as a solid phase II; the leachate comprises at least one of an ether, an alcohol, and water;
(5) Carrying out desolventizing treatment on the ether phase I obtained in the step (4) to obtain a desolventized oil phase II, wherein distillate is ether in the leaching liquor;
(6) Performing solid-liquid separation on the solid phase II obtained in the step (4) after extrusion treatment to obtain liquid phase II and solid phase III;
(7) Mixing the alcoholic solution II obtained in the step (4), the liquid phase II obtained in the step (6) and the alcoholic solution I obtained in the step (3) to obtain a liquid phase III; the volume ratio of the sum of the volumes of the alcohol solution II and the liquid phase II to the alcohol solution I is as follows: (liquid phase II + alcohol solution II): alcoholic solution i = (1.5-2): 1;
(8) Precipitating and separating the liquid phase III obtained in the step (7) to obtain a solid peony protein, and a liquid peony protein mixed solution containing functional substances; the functional substance comprises at least one of melanin, paeonol and paeoniflorin.
(9) Mixing the oil phase I obtained in the step (3) and the oil phase II obtained in the step (5) to obtain peony seed crude oil; the peony seed crude oil comprises at least one of phytosterol, vitamin E and squalene.
Compared with the prior art, the method has the advantages that the peony seeds do not need to be dried in the process flow, the process flow is simple, the cost is reduced, and meanwhile, the effective components in the peony seeds are retained to the greatest extent. Furthermore, the invention realizes the wet continuous grading treatment of the fresh peony seeds through a simple process flow, wherein the special leaching process overcomes the defects of toxicity, harm and high boiling point of the traditional petroleum ether leaching liquor, and realizes the cold rolling of the peony seed oil with low boiling point less than 50 ℃ and low energy consumption. The special leaching liquor can also effectively extract the functional components such as phytosterol, vitamin E, squalene and the like in the peony seeds and the initial-pressed residual peony seed oil, so that the yield of the peony seed oil is improved, the contents of the functional components such as phytosterol, vitamin E, squalene and the like in the peony seed oil are obviously improved, and the peony seed oil with the function of reducing blood fat is produced. The special leaching liquor can also effectively extract substances such as melanin, paeonol, paeoniflorin and the like in peony seeds, so that peculiar smell and variegation in crude oil are reduced. Meanwhile, the bitter substances such as paeoniflorin and the like in the peony seeds are extracted through alcohols in the leaching liquor, and the melanin is dissolved and extracted through water, so that the solid phase III obtained and the solid containing carbohydrate has no bitter taste and black color, and the palatability of the solid phase product in the fields of food and feed is obviously improved. The invention also realizes the automatic precipitation of the peony seed protein and improves the efficiency of continuous production by the volume ratio of the mixture of liquid phase products with different ethanol concentrations in the continuous production. In the invention, the solvents such as alcohol and ether can be recycled through desolventizing and separating, thereby reducing the production cost.
As a preferred embodiment of the present invention, in step (1), the conditions for washing the peony seeds with water are as follows: stirring peony seeds in water of 30-45 deg.C at 100-150rpm for 10-30min.
In a preferred embodiment of the present invention, in the step (2), the solvent is absolute ethanol or an ethanol aqueous solution having a volume fraction of 95% or more.
The inventor finds that the solvent is absolute ethyl alcohol or an ethanol water solution with the volume fraction of more than 95%, can be used for extracting substances such as melanin, paeonol, paeoniflorin and the like from peony seeds in a fusion manner, and can effectively remove peculiar smell and variegated colors in crude oil.
As a preferred embodiment of the present invention, in the step (2), the volume ratio of the solvent to the mixture is (1-2): 1.
the inventor finds that when the volume ratio of the solvent to the mixture of the fusion extraction is (1-2): 1, the peculiar smell and the variegated color in the crude oil can be reduced to the maximum extent. Outside the scope of the present invention, too little solvent is added to ensure the removal of off-flavors and off-colors, while too much solvent results in an increase in cost.
As a preferred embodiment of the present invention, in the step (4), the leaching solution is diethyl ether, ethanol and water.
As a preferred embodiment of the present invention, in the step (4), the volume ratio of the diethyl ether to the ethanol to the water in the leaching solution is: ether: ethanol: water = (4-6): (2-3): (2-3).
As a preferred embodiment of the present invention, in the step (4), the volume ratio of the diethyl ether to the ethanol to the water in the leaching solution is: ether: ethanol: water =6:2:2.
the inventor finds that when the volume of the diethyl ether in the solvent is less than 60 percent, functional components such as phytosterol, vitamin E, squalene and the like in the solid phase I cannot be completely leached due to insufficient amount of the diethyl ether, so that the content of the functional components in the oil phase II is reduced, the waste of the functional components is caused, and the blood lipid reducing function of the peony seed oil product is weakened. When the volume of the ether in the solvent is more than 60 percent, the leaching effect of the functional components is ensured due to the addition of the excessive ether, but the desolventizing time is prolonged due to the excessive ether in the ether phase I, and the efficiency of continuous production is reduced. Preferred ethers of the invention: ethanol: water =6:2:2 hours, a balance of time efficiency and leaching effect is achieved.
As a preferred embodiment of the invention, in the step (4), the mass ratio of the leaching solution to the solid phase I is (1.5-3): 1.
the inventor finds that the mass ratio of the leaching solution to the solid phase I is (1.5-3): 1, the functional components such as phytosterol, vitamin E, squalene and the like in the peony seed oil can be extracted to the maximum extent, and the peony seed oil with the function of reducing blood fat is produced. Outside the scope of the present invention, too little solvent is added to ensure the yield of the functional ingredient, while too much solvent results in an increase in cost.
As a preferred embodiment of the present invention, in the step (5), the conditions of the desolvation are: rotary evaporating at 35-47 deg.C for 60-120min.
The inventor finds that the ether component in the special leaching liquor can separate out the functional components such as phytosterol, vitamin E, squalene and the like by desolventizing at the temperature of below 50 ℃, and the ether can be recovered and continuously used for production.
In a preferred embodiment of the present invention, in the step (5), the desolventizing conditions are: rotary evaporation at 45 ℃ for 120min.
As a preferred embodiment of the present invention, in the step (7), the volume fraction of ethanol in the liquid phase iii is more than 70%.
The inventor finds out through research that the ethanol content in the leaching liquor of the invention is as follows: water =2:2, the volume fraction of the ethanol in the liquid phase II and the alcoholic solution II is about 50%, and the volume fraction of the ethanol in the alcoholic solution I is more than 95%, when the three are as follows (liquid phase II + alcoholic solution II): alcoholic solution i = (1.5-2): 1, the volume fraction of ethanol in the obtained liquid phase III is more than 70%, the peony protein can be naturally separated out, and ethanol is not required to be additionally added to adjust the ethanol concentration of the protein separation condition. The invention is only controlled by (liquid phase II + alcoholic solution II): the volume ratio of the alcoholic solution I can realize the automatic precipitation of protein, thereby reducing the cost and the process complexity.
The invention also provides application of any one of the methods in peony seed treatment.
Compared with the prior art, the invention has the beneficial effects that:
(1) The method can realize continuous production of the peony seed oil, the byproducts are reasonably and efficiently utilized, and the byproducts generated after continuous production are directly classified, so that the subsequent utilization is facilitated.
(2) According to the invention, the substances such as melanin, paeonol and paeoniflorin in the peony seeds can be extracted by fusing the ethanol added during pre-pressing, so that the peculiar smell and the variegated color in the crude oil are reduced to the greatest extent.
(3) The temperature of the production process of the peony seed oil is less than 50 ℃, the whole process of cold pressing is realized, and when the oil phase II is extracted by a leaching method, the used leaching solution can furthest extract functional components in the peony seed oil, wherein the functional components comprise phytosterol, vitamin E, squalene and the like, so that the peony seed oil with the function of reducing blood fat is produced.
(4) The invention is based on a multi-component extraction method, and the leaching solution is different from a common leaching method because the sterol substances specific to the peony seeds can be better dissolved in the leaching solution, and the sterol substances have lower boiling points during the desolventizing, so that the energy consumption is reduced.
(5) The raw materials of the invention are fresh peony seeds, the solvent in the whole process can be recovered, and the obtained product has low solvent residue.
Drawings
FIG. 1 is a schematic view of the process of the present invention.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples. In the examples, unless otherwise specified, the experimental methods are all conventional methods; unless otherwise indicated, the reagents and materials are commercially available.
Example 1
As an embodiment of the method for continuously grading peony seeds, the method comprises the following specific implementation steps:
(1) Stirring fresh peony seeds with purified water of 45 ℃ at the rotating speed of 100rpm for 30min for cleaning, and draining;
(2) Crushing the clean peony seeds obtained in the step (1), physically squeezing to obtain a mixture, adding absolute ethyl alcohol with the same volume as the mixture, mixing, stirring and leaching at the speed of 50r/min for fusion extraction for 30min, and performing solid-liquid separation by using a spiral discharge centrifuge to obtain a solid phase and a liquid phase substance, wherein the solid phase is a solid phase I, and the liquid phase is a liquid phase I.
(3) And (3) separating an alcoholic solution and an oil phase substance obtained 1h after the liquid phase I obtained in the step (2) is kept stand, wherein the alcoholic solution substance is the alcoholic solution I, and the oil phase substance is the oil phase I.
(4) Adding 2.4 times of diethyl ether into the solid phase substance obtained in the step (2): ethanol: water =6:2:2, and standing for 1 hour to obtain an ether phase, an alcoholic solution and a solid phase, wherein the obtained ether phase is an ether phase I, the alcoholic solution is an alcoholic solution II and the solid phase is a solid phase II.
(5) And (5) carrying out rotary evaporation treatment on the ether phase I obtained in the step (4) at 45 ℃ for 2 hours to obtain an oil phase substance, wherein the obtained oil phase substance is an oil phase II, and recovering ether.
(6) And (5) carrying out solid-liquid separation on the solid phase II obtained in the step (4), wherein the obtained solid phase substance is a solid phase III, the obtained liquid phase substance is a liquid phase II, and the solid phase III is mainly carbohydrate.
(7) And (5) mixing the alcoholic solution II obtained in the step (4) with the liquid phase II obtained in the step (6), adding the alcoholic solution I obtained in the step (3) in equal proportion, and mixing to obtain a liquid phase III. The sum of the volume of the alcoholic solution II and the volume ratio of the liquid phase II to the alcoholic solution I are as follows: (liquid phase II + alcohol solution II): alcoholic solution i = (1.5-2): 1. the concentration of ethanol in the obtained liquid phase III is more than 70%.
(8) Performing solid-liquid separation on the liquid phase III obtained in the step (7) to obtain a solid peony protein, and a liquid mixed solution containing functional substances; the functional substances include melanin, paeonol, and paeoniflorin.
(9) And (4) mixing the oil phase I obtained in the step (3) with the oil phase II obtained in the step (5) to obtain the peony seed crude oil.
Example 2
As an embodiment of the method for continuously grading peony seeds, the method comprises the following specific implementation steps:
(1) Stirring fresh peony seeds with purified water of 30 ℃ at the rotating speed of 150rpm for 10min, cleaning, and draining;
(2) Crushing the clean peony seeds obtained in the step (1), physically squeezing to obtain a mixture, adding 95% ethanol with 2 times of the volume of the mixture, mixing, stirring and leaching at the speed of 50r/min, performing fusion extraction for 30min, and performing solid-liquid separation by using a spiral discharge centrifuge to obtain a solid phase and a liquid phase substance, wherein the solid phase is a solid phase I, and the liquid phase is a liquid phase I.
(3) And (3) separating an alcoholic solution and an oil phase substance obtained 1h after the liquid phase I obtained in the step (2) is kept stand, wherein the alcoholic solution substance is the alcoholic solution I, and the oil phase substance is the oil phase I.
(4) Adding 1.5 times of diethyl ether into the solid phase substance obtained in the step (2): ethanol: water =4:2:2, and standing for 1 hour to obtain an ether phase, an alcoholic solution and a solid phase, wherein the obtained ether phase is an ether phase I, the alcoholic solution is an alcoholic solution II and the solid phase is a solid phase II.
(5) And (5) carrying out rotary evaporation treatment on the ether phase I obtained in the step (4) at 35 ℃ for 2 hours to obtain an oil phase substance, wherein the obtained oil phase substance is an oil phase II, and recovering ether.
(6) And (4) carrying out solid-liquid separation on the solid phase II obtained in the step (4), wherein the obtained solid phase substance is a solid phase III, the obtained liquid phase substance is a liquid phase II, and the solid phase III is mainly carbohydrate.
(7) And (3) mixing the alcoholic solution II obtained in the step (4) and the liquid phase II obtained in the step (6), adding the alcoholic solution I obtained in the step (3) in equal proportion, and mixing to obtain a liquid phase III. The volume sum of the alcoholic solution II and the volume ratio of the liquid phase II to the alcoholic solution I is as follows: (liquid phase II + alcohol solution II): alcoholic solution i = (1.5-2): 1. the concentration of ethanol in the obtained liquid phase III is more than 70%.
(8) Performing solid-liquid separation on the liquid phase III obtained in the step (7) to obtain a solid peony protein, and a liquid mixed solution containing functional substances; the functional substances include melanin, paeonol, and paeoniflorin.
(9) And (4) mixing the oil phase I obtained in the step (3) with the oil phase II obtained in the step (5) to obtain the peony seed crude oil.
Example 3
As an embodiment of the method for continuously grading peony seeds, the method comprises the following specific implementation steps:
(1) Stirring fresh peony seeds with purified water of 40 ℃ at the rotating speed of 130rpm for 20min, cleaning, and draining;
(2) Crushing the clean peony seeds obtained in the step (1), physically squeezing to obtain a mixture, adding 98% ethanol with the volume 1.5 times of that of the mixture, mixing, stirring and leaching at 50r/min, performing fusion extraction for 30min, and performing solid-liquid separation by using a spiral discharge centrifuge to obtain a solid phase and a liquid phase substance, wherein the solid phase is a solid phase I, and the liquid phase is a liquid phase I.
(3) And (3) separating an alcoholic solution and an oil phase substance obtained 1h after the liquid phase I obtained in the step (2) is kept stand, wherein the alcoholic solution substance is the alcoholic solution I, and the oil phase substance is the oil phase I.
(4) Adding 3 times of diethyl ether into the solid phase substance obtained in the step (2): ethanol: water =5:3:3, mixing the leaching liquor, standing for 1h to obtain an ether phase, an alcoholic solution and a solid phase, wherein the obtained ether phase is an ether phase I, the alcoholic solution is an alcoholic solution II and the solid phase is a solid phase II.
(5) And (3) carrying out rotary evaporation treatment on the ether phase I obtained in the step (4) at 47 ℃ for 1h to obtain an oil phase substance, wherein the obtained oil phase substance is an oil phase II, and recovering ether.
(6) And (5) carrying out solid-liquid separation on the solid phase II obtained in the step (4), wherein the obtained solid phase substance is a solid phase III, the obtained liquid phase substance is a liquid phase II, and the solid phase III is mainly carbohydrate.
(7) And (3) mixing the alcoholic solution II obtained in the step (4) and the liquid phase II obtained in the step (6), adding the alcoholic solution I obtained in the step (3) in equal proportion, and mixing to obtain a liquid phase III. The volume sum of the alcoholic solution II and the volume ratio of the liquid phase II to the alcoholic solution I is as follows: (liquid phase II + alcohol solution II): alcoholic solution i = (1.5-2): 1. the concentration of ethanol in the obtained liquid phase III is more than 70 percent.
(8) Performing solid-liquid separation on the liquid phase III obtained in the step (7) to obtain a solid which is peony protein, and a liquid which is a mixed solution containing functional substances; the functional substances include melanin, paeonol, and paeoniflorin.
(9) And (4) mixing the oil phase I obtained in the step (3) with the oil phase II obtained in the step (5) to obtain the peony seed crude oil.
Comparative example 1
As a comparative example of the method for continuously treating peony seeds in a grading manner, in comparative example 1, except that the leaching solution in the step (4) is diethyl ether: ethanol: water =10:2:2 the process parameters were the same for all steps except that they were the same as in example 1. In practical production, the increase of the ether content in the leaching liquor can cause the desolventizing time of the step (5) to be prolonged, the ether can not be completely separated by the rotary evaporation treatment for 2 hours at the temperature of 45 ℃, and the ether can not be completely separated by prolonging the rotary evaporation treatment time to 5 hours.
The inventors calculated the yields of the above-mentioned various products by calculating the mass ratio of the carbohydrates, proteins, functional ingredients and oils of the products obtained in examples 1 to 3 and comparative example 1 to the mass of the input peony seed material. The sum of the above product yields is the overall recovery, and the detailed results are shown in table 1 below:
TABLE 1 yield and overall recovery of various products from examples 1-3 and comparative example 1
The results in Table 1 show that the excessive content of ether in the leaching solution of the invention prolongs the desolventizing separation time, and increases the time and energy consumption cost. Preferred ethers of the invention: ethanol: water =6:2:2 hours, a balance of time efficiency and leaching effect is achieved. Meanwhile, the continuous grading treatment method can continuously and efficiently treat the peony seeds to obtain various components such as carbohydrates, peony proteins, functional components, peony seed oil and the like, and the comprehensive recovery rate reaches more than 87%.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (5)
1. A method for continuously grading peony seeds, comprising the steps of:
(1) Washing peony seeds with water, and draining; the peony seeds are fresh peony seeds which are not dried;
(2) Crushing and squeezing the peony seeds obtained in the step (1) to obtain a mixture, adding a solvent into the mixture, performing fusion extraction to obtain a feed liquid, and performing solid-liquid separation on the feed liquid to obtain a solid phase I and a liquid phase I respectively; the fusion extraction is to mix the solvent and the mixture and leach the mixture evenly;
(3) Standing and layering the liquid phase I obtained in the step (2), wherein the separated lower layer is an alcohol solution I, and the upper layer is an oil phase I;
(4) Uniformly mixing the solid phase I obtained in the step (2) with the leaching liquor, standing for layering, separating out an ether phase I as the upper layer of liquid, an alcohol solution II as the lower layer of the liquid, and taking the residual solid as a solid phase II;
(5) Carrying out desolventizing treatment on the ether phase I obtained in the step (4) to obtain a desolventized oil phase II, wherein distillate is ether in the leaching liquor;
(6) Performing solid-liquid separation on the solid phase II obtained in the step (4) after extrusion treatment to obtain liquid phase II and solid phase III; the solid phase III comprises a carbohydrate;
(7) Mixing the alcoholic solution II obtained in the step (4), the liquid phase II obtained in the step (6) and the alcoholic solution I obtained in the step (3) to obtain a liquid phase III; the volume ratio of the sum of the volumes of the alcohol solution II and the liquid phase II to the alcohol solution I is as follows: (liquid phase II + alcohol solution II): alcoholic solution i = (1.5-2): 1;
(8) Precipitating and separating the liquid phase III obtained in the step (7) to obtain a solid peony protein, and a liquid peony protein mixed solution containing functional substances; the functional substance comprises at least one of melanin, paeonol and paeoniflorin;
(9) Mixing the oil phase I obtained in the step (3) and the oil phase II obtained in the step (5) to obtain peony seed crude oil; the peony seed crude oil comprises at least one of phytosterol, vitamin E and squalene;
in the step (2), the solvent is absolute ethyl alcohol or an ethanol water solution with a volume fraction of more than 95%, and the volume ratio of the solvent to the mixture is (1-2): 1; in the step (4), the leaching liquor is diethyl ether, ethanol and water, and the volume ratio of the diethyl ether to the ethanol to the water in the leaching liquor is as follows: diethyl ether: ethanol: water = (4-6): (2-3): (2-3); the mass ratio of the leaching liquor to the solid phase I is (1.5-3): 1.
2. the process of claim 1, wherein in the step (4), the volume ratio of the diethyl ether to the ethanol to the water in the leaching solution is: diethyl ether: ethanol: water =6:2:2.
3. the method according to claim 1, wherein in the step (5), the desolventizing condition is: rotary evaporating at 35-47 deg.C for 60-120min.
4. The process of claim 1, wherein in step (7), the volume fraction of ethanol in the liquid phase III is greater than 70%.
5. Use of the method according to any one of claims 1 to 4 for the treatment of peony seeds.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1081640A (en) * | 1964-12-03 | 1967-08-31 | John Charles Cavanagh | Process for the treatment of vegetable materials |
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