CN107212425A - A kind of method for extracting rice bran dietary fiber - Google Patents
A kind of method for extracting rice bran dietary fiber Download PDFInfo
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- CN107212425A CN107212425A CN201710322598.XA CN201710322598A CN107212425A CN 107212425 A CN107212425 A CN 107212425A CN 201710322598 A CN201710322598 A CN 201710322598A CN 107212425 A CN107212425 A CN 107212425A
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- rice bran
- dietary fiber
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
- A23L7/107—Addition or treatment with enzymes not combined with fermentation with microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/115—Cereal fibre products, e.g. bran, husk
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to food processing field, and in particular to a kind of extracting method of rice bran dietary fiber.This method is to carry out separation and Extraction to defatted rice bran using the two enzymes method of alpha-amylase and protease, can obtain up to 37.6% yield and 89% purity.The extracting method of this dietary fiber, on the one hand equipment component requirement can be saved, it is simple to operate, time-consuming short, on the other hand the mild condition of dietary fiber is prepared, yield and the higher rice bran dietary fiber of purity can be obtained, the comprehensive utilization of rice bran value-added product is realized, the fields such as food, health products are can be widely applied to.
Description
Technical field
The invention belongs to food processing field, and in particular to a kind of extracting method of rice bran dietary fiber.
Background technology
Rice bran is the principal by product of paddy processing, rich in the physiology such as dietary fiber (Dietary fiber, DF) and polyphenol
Active material, is a kind of high added value resource of great exploitation potential.But fresh rice bran easily becomes sour, bin stability is poor, and
And due to wherein containing substantial amounts of dietary fiber, cause its low dissolubility, coarse mouthfeel, working properties poor, seriously constrain rice
Application of the chaff in food.China is the first Chan Dao big countries in the world, produces about 200,000,000 tons of paddy per year, rice bran can be obtained about after processing
18000000 tons, rice bran development and application potentiality are very huge.Although China's rice bran aboundresources, current overwhelming majority rice bran quilts
Feed as livestock and poultry, the rice bran that only there are about 10%~15% is used as liquefaction or to extract phytic acid calcium, inositol, oryzanol contour attached
Value-added product, remaining major part is used as feed, or even is abandoned as discarded object, causes environmental pollution and low in economic efficiency.
Therefore, rice bran product is developed, the comprehensive utilization value of rice bran is improved, with important economical, social and ecological significance.
Dietary fiber refers to that zymolytic plant SNSP class material and lignin etc. can not be digested in human body
High-molecular compound.Water-soluble dietary fiber (Soluble DietaryFiber, SDF) is classified as according to dissolubility and water is not
Soluble dietary fiber (Insoluble DietaryFiber, IDF).Dietary fiber is not yet to be deeply developed and extensively should at present
One of main nutrient composition, is referred to as " the seventh-largest nutrient ", with different physiological roles, including regulation function of intestinal canal,
Suppress serum cholesterol and rise and prevent the big effect of colorectal cancer etc. three, while relevant with diet to high fat of blood, diabetes etc. shows
Generation disease also has important curative effect.
In recent years, with effect of the dietary fiber in the chronic disease such as prevention of cardiovascular disease and obesity increasingly by
To attention, the research to dietary fiber is also more and more extensive.At present, the extraction to dietary fiber that researchers take
Method mainly has Physical, fractionation, enzymatic reagent method, enzyme-chemical reagent combined techniques and fermentation method etc..
Physical is broadly divided into crude separation method and membrane separation process, can preferably retain the activity of dietary fiber, but rice bran
The fiber productivity of middle water-insoluble meals is relatively low, and equipment limit, is unsuitable for industrialized production in addition, so at present at home and abroad
Application it is also very limited, therefore not can apply to actual production；Fractionation technique is simple, and cost is low, but inevitably
Substantial amounts of sewage can be discharged severe contamination is caused to environment, and condition is more violent, be easily destroyed dietary fiber structure, prepare
Diet fiber product usually contains a small amount of protein and starch, and purity is not high, and this method this year is fewer and fewer come what is applied；
Enzyme-chemical-agent technique weak point is that the color and luster of product is deep, and alkali taste is dense, and strong alkali environment also destroys dietary fiber structure, therefore
It is relatively low with product yield made from the method；Enzyme process prepares the method mild condition of dietary fiber, and can save equipment component will
Ask, but technique is more complicated, and the reaction time is longer, although the yield of product is higher, but purity is relatively low, and cost is higher；Hair
Ferment method products obtained therefrom color and luster, smell and purity are better than other method, but because the method is now also in development, technology is still
Prematurity.
The content of the invention
In order to overcome the defect of prior art, the present invention carries out separation and Extraction to defatted rice bran by using two enzymes method, then
Condition is optimized, so as to obtain yield and the of a relatively high rice bran dietary fiber of purity, rice bran is sufficiently integrated
Utilize, be effectively applied to the industries such as food and health products.
To achieve the above object, the present invention provides a kind of method for extracting rice bran dietary fiber, comprises the following steps that：
1) pre-process：Defatted rice bran after stabilization is crushed, it is stand-by that sieving obtains pretreatment rice bran；
2) enzyme hydrolysis starch：Above-mentioned pretreatment rice bran is taken, distilled water is added, PH is adjusted, fire resistant alpha-diastase, vibration is added
Take out, cool down stand-by；
3) enzymatic protein：PH is adjusted, protease is added, go out enzyme after vibration；
4) centrifuge：Lower floor's solid is freeze-dried after centrifugation, and collection obtains insoluble dietary fiber；
5) settle：Lower floor's solid freeze-drying, collection obtains soluble dietary fiber.
It is preferred that, step 1) in defatted rice bran employ n-hexane secondary degreasing method remove fat.
It is preferred that, step 1) in defatted rice bran using Hot-blast Heating method processing raw material rice bran reach stabilization effect.
It is preferred that, step 1) in rice bran cross 40~60 mesh sieves.
It is preferred that, step 2) in fire resistant alpha-diastase mass percent be 1~3%.
It is preferred that, step 2) in the condition of enzyme hydrolysis starch be：PH5~6,3~4h is vibrated at 65~90 DEG C.
It is preferred that, step 3) in protease mass percent be 0.5~2%.
It is preferred that, step 3) in the condition of enzymatic protein be：PH8~8.5,2~3h is vibrated at 50~65 DEG C.
It is preferred that, step 4) in centrifugal condition be：7000~9000rmp, 8~12min.
It is preferred that, step 5) in setting condition be：With the absolute ethyl alcohol settling step 4 of 3~5 times of volumes) centrifugation after
Supernatant, 7.5~8.5h.
The present invention carries out separation and Extraction dietary fiber using the two enzymes method of alpha-amylase and protease to defatted rice bran, its
Beneficial effect is：1) rice bran is expanded into resource utilization and the comprehensive utilization of rice bran as the extraction raw material of dietary fiber
Value；2) two enzymes method prepares the method mild condition of dietary fiber, simple to operate, can save equipment component requirement；3) using should
It is high that method extracts the yield height of rice bran dietary fiber, purity；4) extracting method of the dietary fiber is applied to rice bran, or
Other raw material extract dietary fiber and provide Research Thinking.
Brief description of the drawings
Fig. 1 is insoluble dietary fiber scanning electron microscope (SEM) photograph；
Fig. 2 is soluble dietary fiber scanning electron microscope (SEM) photograph.
Embodiment
The invention will be further elaborated by the following examples, but does not limit the present invention.It is every without departing substantially from the present invention
The change of design or equivalent substitute are included within protection scope of the present invention.
Embodiment 1：
1) crush, sieve：Defatted rice bran after stabilization is crushed, it is stand-by that 40 mesh sieves excessively obtain pretreatment rice bran.
2) enzyme hydrolysis starch：It is accurate to weigh 10g pretreatment rice brans, 100mL distilled water is added, pH is adjusted to 5.5 with hydrochloric acid, plus
Enter the fire resistant alpha-diastase of enzyme dosage 2%, be placed in thermostatic control oscillator vibration, the 3.5h times are vibrated at 75 DEG C of hydrolysis temperature, are taken
Go out, cool down, it is stand-by.
3) enzymatic protein：PH to 8.2 is adjusted with NaOH, the protease of enzyme dosage 2% is added, is placed in thermostatic control oscillator vibration
On, certain time 2.5h is vibrated at a temperature of 60 DEG C, boiling water bath 10min goes out enzyme.
4) centrifuge：8000rmp, 10min.Lower floor's solid is freeze-dried after centrifugation, and collection obtains insoluble dietary fiber
(IDF)。
5) settle：The supernatant after 4) centrifugation is taken to settle 8h, centrifugation, lower floor's solid freezing with the absolute ethyl alcohol of 4 times of volumes
Dry, collection obtains soluble dietary fiber (SDF).
6) determine：The product of preparation is washed to neutrality, filters, after drying under the conditions of 60 DEG C, determines dietary fiber
Content.
Embodiment 2：
1) crush, sieve：Defatted rice bran after stabilization is crushed, it is stand-by that 50 mesh sieves excessively obtain pretreatment rice bran.
2) enzyme hydrolysis starch：It is accurate to weigh 10g pretreatment rice brans, 100mL distilled water is added, pH to 5 is adjusted with hydrochloric acid, is added
The fire resistant alpha-diastase of enzyme dosage 1%, is placed in thermostatic control oscillator vibration, and the 3h times are vibrated at 65 DEG C of hydrolysis temperature, is taken out, cold
But, it is stand-by.
3) enzymatic protein：PH to 8.0 is adjusted with NaOH, the protease of enzyme dosage 0.5% is added, water bath with thermostatic control vibration is placed in
On device, certain time 2h is vibrated at a temperature of 50 DEG C, boiling water bath 8min goes out enzyme.
4) centrifuge：7000rmp, 12min.Lower floor's solid is freeze-dried after centrifugation, and collection obtains insoluble dietary fiber
(IDF)。
5) settle：The supernatant after 4) centrifugation is taken to settle 7.5h with the absolute ethyl alcohol of 3 times of volumes, centrifugation, lower floor's solid is cold
Lyophilized dry, collection obtains soluble dietary fiber (SDF).
6) determine：The product of preparation is washed to neutrality, filters, after drying under the conditions of 55 DEG C, determines dietary fiber
Content.
Embodiment 3：
1) crush, sieve：Defatted rice bran after stabilization is crushed, it is stand-by that 60 mesh sieves excessively obtain pretreatment rice bran.
2) enzyme hydrolysis starch：It is accurate to weigh 10g pretreatment rice brans, 100mL distilled water is added, pH to 6 is adjusted with hydrochloric acid, is added
The fire resistant alpha-diastase of enzyme dosage 3%, is placed in thermostatic control oscillator vibration, and the 4h times are vibrated at 90 DEG C of hydrolysis temperature, is taken out, cold
But, it is stand-by.
3) enzymatic protein：PH to 8.5 is adjusted with NaOH, the protease of enzyme dosage 1% is added, is placed in thermostatic control oscillator vibration
On, certain time 3h is vibrated at a temperature of 65 DEG C, boiling water bath 9min goes out enzyme.
4) centrifuge：9000rmp, 8min.Lower floor's solid is freeze-dried after centrifugation, and collection obtains insoluble dietary fiber
(IDF)。
5) settle：The supernatant after (4) centrifugation is taken to settle 8.5h with the absolute ethyl alcohol of 5 times of volumes, centrifugation, lower floor's solid is cold
Lyophilized dry, collection obtains soluble dietary fiber (SDF).
6) determine：The product of preparation is washed to neutrality, filters, after drying under the conditions of 50 DEG C, determines dietary fiber
Content.
Embodiment 4：Control group 1 is prepared using chemical reagent-enzyme combination partition method
1) crush：Defatted rice bran is crushed using pulverizer, and crosses 40 mesh sieves.
2) soak：The water immersion 4h of 10 times of quality is added, 5min. is boiled
3) digest：Solution is cooled to 65 DEG C, and adds 0.04% enzymolyzing alpha-amylase 40min, the NaOH 4% is dense
Dietary fiber is prepared under degree, alkaline hydrolysis 45min.
4) determine：The product of preparation is washed to neutrality, filters, after drying under the conditions of 60 DEG C, determines dietary fiber
Content.
Embodiment 5：Control group 2 is prepared using combined-enzyme method
1) crush：Defatted rice bran is crushed using pulverizer, and crosses 40 mesh sieves.
2) hydrolysis starch, protein：It is accurate to weigh 10g pretreatment rice brans (butt), 100mL distilled water is added, hydrochloric acid is used
Adjust pH to 5.5, boiling 20min is gelatinized it.3440U/100g mesophilicα-diastases and 4.32AU/100g albumen are added simultaneously
Enzyme, 60-70 DEG C of constant temperature stirs 2.5h, and boiling water bath 10min goes out enzyme.
3) decolourize, after drying and measure rice bran dietary fiber.
As can be seen from Table 1, compared to control group 1 and control group 2, the rice bran meals extracted by the method for embodiment 1,2,3
The purity and yield of fiber are higher, wherein the highest of embodiment 1.Table 2 is as can be seen that what the method for embodiment 1,2,3 was extracted respectively
IDF and SDF contents are also high compared with contrast groups 1 and contrast groups 2, therefore, using the two enzymes method of alpha-amylase and protease to degreasing
Rice bran carries out separation and Extraction, is a kind of gentle, preferable extracting mode, can carry out extensive use.
The comparison of rice bran dietary fiber purity and yield obtained by each embodiment of table 1 and comparative example
The comparison of IDF and SDF contents obtained by each embodiment of table 2 and comparative example
Electron microscopic observation is scanned to the IDF and SDF obtained by this method extraction, as a result such as accompanying drawing 1-2, it can be seen that
IDF and SDF surfaces are in obvious alveolate texture, and SDF honeycomb is more dense in comparison, and both nothings substantially may be used after enzymolysis
The circular starch granules seen, illustrates after double enzymolysis is handled, the purity of dietary fiber is higher, is substantially free of starch impurities.
Alveolate texture is conducive to increasing the specific surface area of dietary fiber, makes the important physicals such as swellability, retention ability, the absorption affinity of fiber
Character is greatly improved.
Claims (10)
1. a kind of method for extracting rice bran dietary fiber, it is characterised in that comprise the following steps：
1) pre-process：Defatted rice bran after stabilization is crushed, it is stand-by that sieving obtains pretreatment rice bran；
2) enzyme hydrolysis starch：Above-mentioned pretreatment rice bran is taken, distilled water is added, PH is adjusted, fire resistant alpha-diastase is added, vibration takes
Go out, cool down stand-by；
3) enzymatic protein：PH is adjusted, protease is added, go out enzyme after vibration；
4) centrifuge：Lower floor's solid is freeze-dried after centrifugation, and collection obtains insoluble dietary fiber；
5) settle：Lower floor's solid freeze-drying, collection obtains soluble dietary fiber.
2. the method according to claim 1 for extracting rice bran dietary fiber, it is characterised in that：The step 1) in degreasing
Rice bran employs n-hexane secondary degreasing method and removes fat.
3. the method according to claim 1 for extracting rice bran dietary fiber, it is characterised in that：The step 1) in degreasing
Rice bran reaches stabilization effect using Hot-blast Heating method processing raw material rice bran.
4. the method according to claim 1 for extracting rice bran dietary fiber, it is characterised in that：The step 1) in rice bran
Cross 40~60 mesh sieves.
5. it is according to claim 1 extract rice bran dietary fiber method, it is characterised in that the step 2) in high temperature resistant
The mass percent of amylase is 1~3%.
6. it is according to claim 1 extract rice bran dietary fiber method, it is characterised in that the step 2) in enzyme hydrolysis
The condition of starch is：PH5~6,3~4h is vibrated at 65~90 DEG C.
7. it is according to claim 1 extract rice bran dietary fiber method, it is characterised in that the step 3) in protease
Mass percent be 0.5~2%.
8. it is according to claim 1 extract rice bran dietary fiber method, it is characterised in that the step 3) in enzyme hydrolysis
The condition of protein is：PH8~8.5,2~3h is vibrated at 50~65 DEG C.
9. it is according to claim 1 extract rice bran dietary fiber method, it is characterised in that the step 4) in centrifugation
Condition is：7000~9000rmp, 8~12min.
10. the method according to claim 1 for extracting rice bran dietary fiber, it is characterised in that the step 5) in it is heavy
Drop condition is：With the absolute ethyl alcohol settling step 4 of 3~5 times of volumes) centrifugation after supernatant, 7.5~8.5h.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007860A (en) * | 2018-06-14 | 2018-12-18 | 中南林业科技大学 | A kind of antioxidative method of raising rice bran soluble dietary fiber |
| CN109303259A (en) * | 2018-11-21 | 2019-02-05 | 中南林业科技大学 | It is a kind of can function of stabilizing body blood sugar after meal cooking Flour product and preparation method thereof |
| CN109601846A (en) * | 2018-10-15 | 2019-04-12 | 南昌大学 | A kind of method of modifying of dietary fiber in defatted rice bran |
| CN109845784A (en) * | 2019-03-08 | 2019-06-07 | 南昌大学 | Application of the soluble dietary fiber of fermentation method preparation in production biscuit |
| CN109998119A (en) * | 2019-04-08 | 2019-07-12 | 吕梁学院 | A kind of preparation of milled glutinous broomcorn millet dietary fiber and discoloration method |
| CN110495612A (en) * | 2019-09-17 | 2019-11-26 | 广东海洋大学 | A kind of production method of seawater rice chaff cellulose powder |
| CN111011873A (en) * | 2019-12-30 | 2020-04-17 | 长沙理工大学 | Modification method of rice bran dietary fiber |
| CN111938162A (en) * | 2020-08-31 | 2020-11-17 | 四川饭统生物食品有限公司 | Method for extracting dietary fiber by using defatted rice bran |
| CN116687005A (en) * | 2023-03-02 | 2023-09-05 | 武汉轻工大学 | Rice bran cellulose nanocrystalline, preparation method thereof and medicinal diet composition for regulating blood sugar of organism |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007860A (en) * | 2018-06-14 | 2018-12-18 | 中南林业科技大学 | A kind of antioxidative method of raising rice bran soluble dietary fiber |
| CN109007860B (en) * | 2018-06-14 | 2021-09-14 | 中南林业科技大学 | Method for improving oxidation resistance of rice bran soluble dietary fiber |
| CN109601846A (en) * | 2018-10-15 | 2019-04-12 | 南昌大学 | A kind of method of modifying of dietary fiber in defatted rice bran |
| CN109601846B (en) * | 2018-10-15 | 2022-06-14 | 南昌大学 | Modification method of dietary fiber in defatted rice bran |
| CN109303259A (en) * | 2018-11-21 | 2019-02-05 | 中南林业科技大学 | It is a kind of can function of stabilizing body blood sugar after meal cooking Flour product and preparation method thereof |
| CN109845784A (en) * | 2019-03-08 | 2019-06-07 | 南昌大学 | Application of the soluble dietary fiber of fermentation method preparation in production biscuit |
| CN109998119A (en) * | 2019-04-08 | 2019-07-12 | 吕梁学院 | A kind of preparation of milled glutinous broomcorn millet dietary fiber and discoloration method |
| CN110495612A (en) * | 2019-09-17 | 2019-11-26 | 广东海洋大学 | A kind of production method of seawater rice chaff cellulose powder |
| CN111011873A (en) * | 2019-12-30 | 2020-04-17 | 长沙理工大学 | Modification method of rice bran dietary fiber |
| CN111938162A (en) * | 2020-08-31 | 2020-11-17 | 四川饭统生物食品有限公司 | Method for extracting dietary fiber by using defatted rice bran |
| CN116687005A (en) * | 2023-03-02 | 2023-09-05 | 武汉轻工大学 | Rice bran cellulose nanocrystalline, preparation method thereof and medicinal diet composition for regulating blood sugar of organism |
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Application publication date: 20170929 |