CN113383673A - Preparation method of poria cocos planting nutrition bag - Google Patents
Preparation method of poria cocos planting nutrition bag Download PDFInfo
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- CN113383673A CN113383673A CN202110764600.5A CN202110764600A CN113383673A CN 113383673 A CN113383673 A CN 113383673A CN 202110764600 A CN202110764600 A CN 202110764600A CN 113383673 A CN113383673 A CN 113383673A
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a preparation method of a poria cocos planting nutrition bag, and relates to the technical field of poria cocos. The method comprises the following steps: s1, selecting strains; s2, culturing hyphae of the stock; s3, cultivating hyphae of the cultivated species; s4, guiding wood to select; s5, preparing cultivars; s6, preparing a nutrition bag; s7, inoculating and culturing the nutrition bag; s8, soil preparation and land selection; s9, grafting; s10, planting; and S11, performing later management. According to the preparation method of the poria cocos planting nutrition bag, the cultivation effect of the nutrition bag is improved under the matching of the nutrition bag and the cultivation bag, sufficient nutrient substances are provided for the growth of poria cocos, the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved, the prepared nutrition medium is pure natural, pollution-free and rich in various nutrients, and the cultivated poria cocos is high in growth speed, developed in root, good in disease resistance and strong in pest and disease resistance.
Description
Technical Field
The invention relates to the technical field of poria cocos nutrition packages, in particular to a preparation method of a poria cocos planting nutrition package.
Background
Poria, the name of Chinese medicine, is the dried sclerotium of Poria of Polyporaceae fungus, it contains pachymaran, glucose, protein, amino acid, organic acid, fat, lecithin, adenine, choline, ergosterol, many enzymes and many ingredients such as potassium salt, etc., it has effects of excreting dampness, diuresis, invigorating the spleen, tranquilization, etc., its medicinal value lies in: has effects in enhancing immunity, resisting tumor, promoting urination, increasing discharge of electrolyte such as potassium, sodium, and chlorine, tranquilizing mind, protecting liver, inhibiting ulcer, lowering blood sugar, and resisting radiation, and can be used for treating edema, fullness, phlegm and fluid retention, cough, vomiting, diarrhea, nocturnal emission, stranguria with turbid urine, palpitation, and amnesia.
In the prior art, in order to improve the yield of poria cocos in the planting process, namely, a fresh poria cocos is grafted at the lower end of a pine section full of hypha and used for guiding the growth of the poria cocos, the time required for grafting combination of the fresh poria cocos block and the pine section is long, but the poria cocos is cultivated in the open air, the probability of rainwater climate is increased in advance if the combination time is long, and the survival rate of the poria cocos block is low once the poria cocos meets rainwater weather, so that the production yield is improved by using a nutrition bag for culture in a matched mode in the planting process of the poria cocos, but the conventional nutrition bag provides common nutrients for the poria cocos in the culture process.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of a poria cocos planting nutrition bag, and aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a poria cocos planting nutrition bag comprises the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, inoculating the poria cocos flesh in the poria cocos blocks to a culture medium I in a triangular flask, transferring the poria cocos blocks to a thermostat with the temperature of 22-25 ℃ for culturing for 5-7 days until mycelium grows in a culture container, and obtaining required stock hypha;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn hyphae prepared in the step S2 onto a culture medium II in a polypropylene cultivation bag with the diameter of 6cm, and performing cultivation operation until the cultivation bag is full of poria cocos hyphae, so as to obtain cultivation spawn hyphae;
s4, wood guiding operation: peeling pine tree branches with the diameter of 3-5cm and the length of 30-40cm, air-drying, soaking in saturated brown sugar water for a day and a night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of the hyphae of the cultivated species obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 15-30 ℃ for 10-15 days for later use;
s6, preparing the nutrition packet: mixing 20% of pine sawdust, 25% of pine chips, 15% of pine wool, 15% of corn meal, 20% of wheat bran, 1% of brown sugar, 1% of gypsum and 3% of soybean meal according to parts by weight, and then carrying out disinfection treatment to prepare the required nutrition bag;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 ℃ for 30-40 days;
s8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field in sandy soil with the soil mainly comprising yellow sandy soil and the pH value of 3-5, and spraying a sterilizing liquid to the cultivation pits to perform disinfection and insect killing treatment after excavation;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken and inoculated on the cross section of the leading wood and wrapped by an agricultural film;
s10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
Further optimizing the technical scheme, the nutrition bag prepared in the step S6 is prepared by adding water into pine wood chips, pine nuts, corn flour, wheat bran, brown sugar, gypsum and bean pulp, uniformly mixing, and controlling the water content to be 50-55%.
Further optimizing the technical scheme, the culture bag in the step S5 is cultured for 13-18 days at the constant temperature of 10-15 ℃, and then is kept at the constant temperature of 25-30 ℃ for 5-12 days until the culture bag is full of mycelia, so that the required cultivated species are obtained.
Further optimizing the technical scheme, in the step S7, in the process of inoculation treatment, an opening is cut in the strain bag, a new opening is sawed at one end of the nutrition bag close to the strain bag, and the opening of the strain bag is closely attached to the new opening of the nutrition bag.
Further optimizing the technical scheme, the size of the nutrition package in the step S7 is 60-70cm in length, 25-35cm in width and 0.2-0.4cm in thickness.
Further optimizing the technical scheme, the first culture medium in S2 is 80-120 parts of sweet potato, 30-40 parts of glucose, 20-25 parts of agar, l-3 parts of monopotassium phosphate and 0.5-2 parts of ferrous sulfate, and water is added for mixing to ensure that the water content is 50-60%.
Further optimizing the technical scheme, in the step S3, the second culture medium is 10-18 parts of gypsum powder, 35-45 parts of corn straw, 6-10 parts of water and 5-10 parts of soybean meal, and the mixture is treated to ensure that the water content is 55-75%.
Further optimizing the technical scheme, in the step S5, the weight of the culture medium raw material in each bag of culture bag is 2 kg.
Further optimizing the technical scheme, in the step S8, cultivation pits need to be dug in the planting field, the depth of the dug pits is 30-40cm, and the width of the dug pits is 60-80 cm.
Compared with the prior art, the invention provides a preparation method of the poria cocos planting nutrition bag, which has the following beneficial effects:
1. according to the preparation method of the poria cocos planting nutrition bag, the cultivation effect of the nutrition bag is improved under the matching of the nutrition bag and the cultivation bag, sufficient nutrient substances are provided for the growth of poria cocos, the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved, the prepared nutrition medium is pure natural, pollution-free and rich in various nutrients, and the cultivated poria cocos is high in growth speed, developed in root, good in disease resistance and strong in pest and disease resistance.
2. According to the preparation method of the poria cocos planting nutrition package, the poria cocos is covered with soil, and meanwhile, the guide wood is covered with the rice straw or the thin film, so that the effect of preventing the poria cocos from insect damage is improved, the cultivation cost is reduced, the yield of the poria cocos is improved, the preparation method has the advantages of being simple and convenient to operate, stable in yield, short in period and the like, and the production cost is saved.
Drawings
Fig. 1 is a schematic flow chart of a preparation method of a poria cocos planting nutrition bag provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: referring to fig. 1, the invention discloses a preparation method of a poria cocos planting nutrition bag, which comprises the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking the poria cocos blocks selected in the step S1 out of the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos blocks onto a first culture medium in a triangular flask, transferring the poria cocos blocks into a 22 ℃ incubator for culturing for 5 days until mycelium grows in the culture container, wherein the first culture medium is 80 parts of sweet potatoes, 30 parts of glucose, 25 parts of agar, 3 parts of potassium dihydrogen phosphate and 2 parts of ferrous sulfate, and adding water for mixing until the water content is 60% to obtain required stock hypha;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, mixing the second culture medium which is 18 parts of gypsum powder, 45 parts of corn straw, 6 parts of water and 10 parts of soybean meal to ensure that the water content is 75%, and carrying out cultivation until the cultivation bag is full of poria cocos mycelium to obtain cultivation spawn;
s4, wood guiding operation: peeling and air-drying pine branches with the diameter of 5cm and the length of 40cm, soaking the pine branches in saturated brown sugar water for one day and night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag in which the hyphae of the cultivated species are obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, culturing and propagating at the temperature of 30 ℃ for 15 days for later use, culturing the culture bag at the constant temperature of 15 ℃ for 13 days, then, maintaining the constant temperature at the temperature of 30 ℃ for 12 days until the culture bag is full of the hyphae, wherein the weight of the culture medium raw material in each culture bag is 2kg, so as to obtain the required cultivated species;
s6, preparing the nutrition packet: mixing 20% of pine sawdust, 25% of pine chips, 15% of pine hair, 15% of corn meal, 20% of wheat bran, 1% of brown sugar, 1% of gypsum and 3% of soybean meal according to parts by weight, controlling the water content to be 55%, and performing disinfection treatment after mixing to prepare the required nutrition bag;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the step S5 into the nutrition bag prepared in the step S6 under aseptic conditions, culturing for 40 days at 28 ℃, cutting an opening of the strain bag during inoculation treatment, sawing one end of the nutrition bag close to the strain bag into a new opening, and tightly attaching the opening of the strain bag and the new opening of the nutrition bag, wherein the size of the nutrition bag is 70cm in length, 35cm in width and 0.4cm in thickness;
s8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field by using sandy soil with the soil mainly including yellow sandy soil and the pH value of 5, excavating the cultivation pits in the planting field, wherein the depth of the excavated pits is 40cm, the width of the excavated pits is 80cm, and spraying a disinfectant and insecticidal solution to the cultivation pits to disinfect and kill insects after the excavation is finished;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken and inoculated on the cross section of the leading wood and wrapped by an agricultural film;
s10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: and (3) checking the growth condition of hyphae in each section 10 days after inoculation so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, covering soil, weeding and killing insects in time, and paying attention to drainage in rainy seasons.
Example two: referring to fig. 1, the invention discloses a preparation method of a poria cocos planting nutrition bag, which comprises the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, taking out the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos meat to a first culture medium in a triangular flask, transferring the first culture medium into a 24-DEG C incubator for culturing for 6 days until mycelium grows in the culture container, wherein the first culture medium is 120 parts of sweet potatoes, 35 parts of glucose, 22 parts of agar, 2 parts of potassium dihydrogen phosphate and 1.5 parts of ferrous sulfate, and adding water for mixing until the water content of the first culture medium is 50% to obtain required stock hyphae;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, mixing the second culture medium which is 15 parts of gypsum powder, 40 parts of corn straw, 8 parts of water and 7 parts of soybean meal to ensure that the water content is 75%, and carrying out cultivation until the cultivation bag is full of poria cocos mycelium to obtain cultivation spawn;
s4, wood guiding operation: peeling and air-drying pine tree branches with the diameter of 4cm and the length of 40cm, soaking the pine tree branches in saturated brown sugar water for one day and night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag in which the hyphae of the cultivated species are obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, culturing and propagating at the temperature of 29 ℃ for 10 days for later use, culturing the culture bag at the constant temperature of 15 ℃ for 13 days, then, maintaining the constant temperature at the temperature of 30 ℃ for 12 days until the culture bag is full of the hyphae, wherein the weight of the culture medium raw material in each culture bag is 2kg, so as to obtain the required cultivated species;
s6, preparing the nutrition packet: mixing 20% of pine sawdust, 25% of pine chips, 15% of pine hair, 15% of corn meal, 20% of wheat bran, 1% of brown sugar, 1% of gypsum and 3% of soybean meal according to parts by weight, controlling the water content to be 55%, and performing disinfection treatment after mixing to prepare the required nutrition bag;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the step S5 into the nutrition bag prepared in the step S6 under aseptic conditions, culturing for 40 days at the temperature of 30 ℃, cutting an opening of the strain bag in the process of inoculation treatment, sawing one end of the nutrition bag close to the strain bag into a new opening, and tightly attaching the opening of the strain bag and the new opening of the nutrition bag, wherein the size of the nutrition bag is 70cm in length, 35cm in width and 0.4cm in thickness;
s8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field by using sandy soil with the soil mainly comprising yellow sandy soil and the pH value of 3, excavating the cultivation pits in the planting field, wherein the depth of the excavated pits is 40cm, the width of the excavated pits is 80cm, and spraying a disinfectant and insecticidal solution to the cultivation pits to disinfect and kill insects after the excavation is finished;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken and inoculated on the cross section of the leading wood and wrapped by an agricultural film;
s10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
Example three: referring to fig. 1, the invention discloses a preparation method of a poria cocos planting nutrition bag, which comprises the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, taking out the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos blocks onto a first culture medium in a triangular flask, transferring the poria cocos blocks into a thermostat at 25 ℃ for culturing for 7 days until mycelium grows in a culture container, wherein the first culture medium is 100 parts of sweet potatoes, 35 parts of glucose, 24 parts of agar, 2 parts of potassium dihydrogen phosphate and 1.5 parts of ferrous sulfate, and adding water for mixing until the water content is 55% to obtain required original seed mycelium;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn hyphae prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, mixing the second culture medium which is 15 parts of gypsum powder, 40 parts of corn straw, 8 parts of water and 8 parts of soybean meal to ensure that the water content is 75%, and carrying out cultivation operation until the cultivation bag is full of poria cocos hyphae, thus obtaining cultivation spawn;
s4, wood guiding operation: peeling and air-drying pine branches with the diameter of 4cm and the length of 35cm, soaking the pine branches in saturated brown sugar water for one day and night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag in which the hyphae of the cultivated species are obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, culturing and propagating at the temperature of 25 ℃ for 12 days for later use, culturing the culture bag at the constant temperature of 12 ℃ for 15 days, then, maintaining the constant temperature at the temperature of 28 ℃ for 7 days until the culture bag is full of the hyphae, wherein the weight of the culture medium raw material in each culture bag is 2kg, so as to obtain the required cultivated species;
s6, preparing the nutrition packet: mixing 20% of pine sawdust, 25% of pine chips, 15% of pine hair, 15% of corn meal, 20% of wheat bran, 1% of brown sugar, 1% of gypsum and 3% of soybean meal according to parts by weight, controlling the water content to be 55%, and performing disinfection treatment after mixing to prepare the required nutrition bag;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the step S5 into the nutrition bag prepared in the step S6 under aseptic conditions, culturing for 40 days at the temperature of 30 ℃, cutting an opening of the strain bag in the process of inoculation treatment, sawing one end of the nutrition bag close to the strain bag into a new opening, and tightly attaching the opening of the strain bag and the new opening of the nutrition bag, wherein the size of the nutrition bag is 70cm in length, 35cm in width and 0.4cm in thickness;
s8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field by using sandy soil with the soil mainly including yellow sandy soil and the pH value of 5, excavating the cultivation pits in the planting field, wherein the depth of the excavated pits is 40cm, the width of the excavated pits is 60cm, and spraying a disinfectant and insecticidal solution to the cultivation pits to disinfect and kill insects after the excavation is finished;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken and inoculated on the cross section of the leading wood and wrapped by an agricultural film;
s10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
And (4) judging the standard: through comparison of the three embodiments, the best effect is the second embodiment, so that the second embodiment is selected as the best embodiment, and the specific change of the amount also belongs to the protection scope of the technical scheme.
The invention has the beneficial effects that: according to the preparation method of the poria cocos planting nutrition bag, the cultivation effect of the nutrition bag is improved under the matching of the nutrition bag and the cultivation bag, so that sufficient nutrient substances are provided for the growth of poria cocos, the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved, the prepared nutrition medium is pure natural, pollution-free and rich in various nutrients, and the cultivated poria cocos is high in growth speed, developed in root, good in disease resistance and strong in pest and disease resistance; according to the method, the poria cocos is covered with soil, and meanwhile, the straw or the thin film is covered on the wood guiding cover, so that the insect pest prevention effect of the poria cocos is improved, the cultivation cost is reduced, the yield of the poria cocos is improved, the method has the advantages of simplicity and convenience in operation, stable yield, short period and the like, and the production cost is saved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A preparation method of a poria cocos planting nutrition bag is characterized by comprising the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, inoculating the poria cocos flesh in the poria cocos blocks to a culture medium I in a triangular flask, transferring the poria cocos blocks to a thermostat with the temperature of 22-25 ℃ for culturing for 5-7 days until mycelium grows in a culture container, and obtaining required stock hypha;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn hyphae prepared in the step S2 onto a culture medium II in a polypropylene cultivation bag with the diameter of 6cm, and performing cultivation operation until the cultivation bag is full of poria cocos hyphae, so as to obtain cultivation spawn hyphae;
s4, wood guiding operation: peeling pine tree branches with the diameter of 3-5cm and the length of 30-40cm, air-drying, soaking in saturated brown sugar water for a day and a night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of the hyphae of the cultivated species obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 15-30 ℃ for 10-15 days for later use;
s6, preparing the nutrition packet: mixing 20% of pine sawdust, 25% of pine chips, 15% of pine wool, 15% of corn meal, 20% of wheat bran, 1% of brown sugar, 1% of gypsum and 3% of soybean meal according to parts by weight, and then carrying out disinfection treatment to prepare the required nutrition bag;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 ℃ for 30-40 days;
s8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field in sandy soil with the soil mainly comprising yellow sandy soil and the pH value of 3-5, and spraying a sterilizing liquid to the cultivation pits to perform disinfection and insect killing treatment after excavation;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken and inoculated on the cross section of the leading wood and wrapped by an agricultural film;
s10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
2. The method for preparing the tuckahoe cultivation nutrition pack according to claim 1, wherein the nutrition pack prepared in the step S6 is prepared by mixing pine wood chips, pine wood pieces, pine nuts, corn flour, wheat bran, brown sugar, gypsum and bean pulp with water, and controlling the water content to be 50-55%.
3. The method for preparing a tuckahoe cultivation nutrition bag according to claim 1, wherein the cultivation bag in the step S5 is cultivated at a constant temperature of 10-15 ℃ for 13-18 days, and then at a constant temperature of 25-30 ℃ for 5-12 days until the cultivation bag is full of mycelia to obtain the desired cultivation seeds.
4. The method as claimed in claim 1, wherein in step S7, during the inoculation process, an opening is cut out from the strain bag, a new opening is sawed into the nutrition bag near one end of the strain bag, and the opening of the strain bag is closely attached to the new opening of the nutrition bag.
5. The preparation method of the poria cocos planting nutrition package according to claim 1, wherein the size of the nutrition package in the step S7 is 60-70cm long, 25-35cm wide and 0.2-0.4cm thick.
6. The preparation method of the poria cocos planting nutrition package as claimed in claim 1, wherein the first culture medium in the S2 is 80-120 parts of sweet potatoes, 30-40 parts of glucose, 20-25 parts of agar, l-3 parts of monopotassium phosphate and 0.5-2 parts of ferrous sulfate, and water is added to mix until the water content is 50-60%.
7. The method for preparing the tuckahoe cultivation nutrition package as claimed in claim 1, wherein the second culture medium in the step S3 is prepared by mixing 10-18 parts of gypsum powder, 35-45 parts of corn straw, 6-10 parts of water and 5-10 parts of soybean meal, so that the water content is 55-75%.
8. The method for preparing a tuckahoe cultivation nutrition bag according to claim 1, wherein the weight of the culture medium raw material in each bag of the culture bag in the step S5 is 2 kg.
9. The method for preparing a tuckahoe planting nutrition bag according to claim 1, wherein in the step S8, a planting pit needs to be dug in the planting field, the depth of the dug pit is 30-40cm, and the width of the dug pit is 60-80 cm.
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