CN113197225A - Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae - Google Patents
Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae Download PDFInfo
- Publication number
- CN113197225A CN113197225A CN202110479222.6A CN202110479222A CN113197225A CN 113197225 A CN113197225 A CN 113197225A CN 202110479222 A CN202110479222 A CN 202110479222A CN 113197225 A CN113197225 A CN 113197225A
- Authority
- CN
- China
- Prior art keywords
- polyphenol
- waste liquid
- plant
- brown algae
- fucoxanthin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae, belonging to the technical field of plant stress resistance physiology, and the resource utilization method comprises the following steps: the polyphenol waste liquid is used for preparing plant stress-resistant foliar fertilizer, the polyphenol waste liquid is used for plant stress-resistant seed soaking liquid, the polyphenol waste liquid is used for preparing plant growth-promoting foliar fertilizer, and the polyphenol waste liquid is used for preparing liquid foliar fertilizer. The method prepares the polyphenol waste liquid into the liquid fertilizer for inducing the stress resistance of plants, can provide a new technology for green production of brown algae industry, which has zero waste discharge and full resource utilization, has environmental protection significance and economic value of high-efficiency agriculture, is a new technology for guaranteeing the stress-resistant safe production of crops, and is a technology urgently needed by related enterprises at present.
Description
Technical Field
The invention relates to the technical field of plant stress resistance physiology, in particular to a resource utilization method of polyphenol waste liquid obtained by extracting fucoxanthin from brown algae.
Background
China is the world with the largest kelp planting and processing scale. According to the annual book of Chinese fishery statistics in 2019, the annual output of kelp in China in 2018 reaches 1522537 tons, and the kelp is the first in the world, and the output of other countries is lower than million tons. Fucoxanthin with high added value can be prepared from brown algae of Laminaria. But a large amount of solid waste seaweed residues and liquid waste are generated in the production process of the fucoxanthin.
The solid waste seaweed residues can be used for producing fertilizers and feeds after being treated, but secondary utilization of liquid waste is not reported. The liquid waste from fucoxanthin production contains rich phenolic compounds, and also contains amino acids, inorganic salts, alginate oligosaccharides, betaine, salicylic acid, abscisic acid, gibberellin and other plant hormones, and can be used as raw materials of plant foliar fertilizer.
The brown algae polyphenol is a common polyphenol compound existing in brown algae, the structure of the brown algae polyphenol compound contains rich phenolic hydroxyl groups, the brown algae polyphenol compound has strong antioxidant activity, and the polyphenol substances in plants can effectively remove superoxide radicals and reduce the damage of plants to organisms under adverse conditions. The alginate oligosaccharide is an oligomer of algin, and the alginate oligosaccharide and the derivative thereof with low polymerization degree have wide biological activities of regulating plant growth, promoting microalgae growth, inducing plant stress resistance and the like. The salicylic acid can also induce the stress resistance of the plant, including the tolerance to various environmental stresses such as salt stress, low-temperature stress, high-temperature stress, drought stress, ultraviolet stress, heavy metal stress and the like, and can also enhance the system immune defense of the plant, improve the immune-related enzyme activity and resist diseases.
Disclosure of Invention
The invention aims to provide a resource utilization method of polyphenol waste liquid from fucoxanthin extraction by brown algae, wherein the waste liquid from fucoxanthin extraction by brown algae contains bio-stimulin such as brown algae polyphenol, brown algae oligosaccharide, jasmonic acid, abscisic acid, gibberellin, quercetin, hydrogen safflower bean acid, myricetin, catechol and the like, and the method can have a remarkable growth promotion effect on stress resistance and growth of cucumber seedlings within a proper concentration range. Therefore, the waste is prepared into the liquid fertilizer for inducing the stress resistance of the plants, a new technology of zero waste discharge and full resource utilization can be provided for the green production of the brown algae industry, the technology has environmental protection significance and high-efficiency agricultural economic value, is a new technology for guaranteeing the stress-resistant and safe production of crops, and is a technology urgently needed by related enterprises at present. Has wide application prospect.
The technical scheme of the invention is realized as follows:
the invention provides a resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae, which comprises the following steps: the polyphenol waste liquid is used for preparing plant stress-resistant foliar fertilizer, the polyphenol waste liquid is used for plant stress-resistant seed soaking liquid, the polyphenol waste liquid is used for preparing plant growth-promoting foliar fertilizer, and the polyphenol waste liquid is used for preparing liquid foliar fertilizer.
As a further improvement of the invention, the application of the polyphenol waste liquid to the preparation of the plant stress-resistant foliar fertilizer specifically comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2, treating cotyledon cucumber seedlings: after the cucumber cotyledon is flattened, spraying 3-4mL of polyphenol waste liquid to the whole plant.
As a further improvement of the invention, the application of the polyphenol waste liquid to the preparation of the plant stress-resistant foliar fertilizer specifically comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2, treating cotyledon cucumber seedlings: after the cucumber cotyledon is flattened, spraying 3-4mL of polyphenol waste liquid to the whole plant.
As a further improvement of the invention, the room temperature is 20-25 ℃.
As a further improvement of the present invention, the plant seed is a cucumber seed or a tomato seed.
As a further improvement of the invention, the specific method for preparing the plant growth promoting foliar fertilizer by using the polyphenol waste liquid comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2.3, treating the plant seedlings at the leaf stage: after the third true leaf of the plant seedling grows, spraying 10-15ml of polyphenol waste liquid per plant on the whole plant.
As a further improvement of the invention, the plant seedling is a cucumber seedling or a tomato seedling.
As a further improvement of the invention, the specific method for using the polyphenol waste liquid for preparing the liquid foliar fertilizer comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2.3, treating cucumber seedlings in leaf stage: spraying 10-15ml of polyphenol waste liquid per plant on the whole plant after the third true leaf of the cucumber seedling grows.
The invention has the following beneficial effects: the invention relates to a method for preparing fucoxanthin from brown algae, which is characterized in that the waste liquid generated by extracting fucoxanthin from brown algae contains biological stimulin such as brown algae polyphenol, brown algae oligosaccharide, jasmonic acid, abscisic acid, gibberellin, quercetin, hydrogen safflower bean acid, myricetin, catechol and the like, and the biological stimulin has obvious growth promotion effect on stress resistance and growth of cucumber seedlings in a proper concentration range. Therefore, the waste is prepared into the liquid fertilizer for inducing the stress resistance of the plants, a new technology of zero waste discharge and full resource utilization can be provided for the green production of the brown algae industry, the technology has environmental protection significance and high-efficiency agricultural economic value, is a new technology for guaranteeing the stress-resistant and safe production of crops, and is a technology urgently needed by related enterprises at present. Has wide application prospect.
1. The invention has obvious improvement on the temperature stress tolerance of the cucumber seedling in the cotyledon period, improves the tolerance of the cucumber seedling at the high temperature of 42 ℃ by 36.67 percent, and improves the tolerance of the cucumber seedling at the low temperature of 4 ℃ by 26.79 percent.
2. The invention has obvious growth promotion effect on cucumber seedlings in 3-leaf stage, and the plant height is improved by 26.46 percent after 14 days of use.
3. The method is beneficial to clean production and resource full utilization of fucoxanthin extracted from brown algae, and 10000 tons of polyphenol waste liquid can be generated when 50% fucoxanthin is produced by 1 ton per ton, so that the method has extremely wide application market.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a graph showing the effect of different concentrations of polyphenol waste solutions of example 1 on the radicle length of cucumber seeds.
FIG. 2 is a graph showing the effect of different concentrations of polyphenol waste liquids of example 1 on the fresh weight of cucumber seeds.
FIG. 3 is a graph showing the effect of different concentrations of polyphenol waste solutions of example 1 on the radicle length of tomato seeds.
FIG. 4 is a graph showing the temperature stress resistance of cucumber seedlings by polyphenol waste liquids according to different modes of application in example 2.
FIG. 5 is a graph of the adverse effect of different modes of use of the polyphenol based waste solutions of example 2 on temperature of tomato seedlings.
FIG. 6 is a graph showing the effect of spraying the whole plant of polyphenol waste liquid with different concentrations in example 3 on the plant height of the cucumber in the trefoil stage.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment is to detect the influence of polyphenol waste liquid generated by extracting fucoxanthin from brown algae on seed germination, and comprises the following steps:
1) treating the polyphenol waste liquid: diluting the polyphenol waste liquid and clear water in the proportion of 1:0, 1:49, 1:99, 1:199 and 0:1, and respectively recording as test one, test two, test three, test four and CK;
2) seed soaking treatment of cucumber and tomato seeds: soaking seeds of cucumbers and tomatoes in polyphenol waste liquid with different concentrations for 24 hours at room temperature (25 ℃);
3) accelerating germination of cucumber and tomato seeds; putting two layers of water-absorbing filter paper into a 9cm culture dish, uniformly putting 20 cucumber seeds and 25 tomato seeds, and carrying out dark culture at 28 ℃ for accelerating germination;
4) determination of germination and radicle growth indexes of cucumber seeds: the germination index is that the length of the embryonic axis breaking through the seed coat reaches half of the full length of the seed. Counting the germination number every day, calculating the germination vigor on the 2 nd day, stopping the germination on the 4 th day, and calculating the germination rate. 20 cucumber seedlings (3 replicates) were selected for each treatment and the full length of the radicle (from the bottom of the radicle to the top of the hypocotyl) was measured; the surface moisture of the cucumber seeds is sucked dry by absorbent paper, and the fresh mass is weighed.
Germination percentage (%) — number of germinated seeds/number of test seeds × 100;
germination potential (%) -48 h germination seed number/test seed number x 100;
germination Index (GI) ═ Σ Gt/Dt (Gt denotes the number of sprouts td, Dt denotes the number of days to sprout);
viability Index (VI) ═ GI × S (GI means germination index, S means average root length).
5) And (3) measuring indexes of tomato seed germination and radicle growth: the germination index is that the length of the embryonic axis breaking through the seed coat reaches half of the full length of the seed. Counting the germination number every day, calculating the germination vigor on the 3 rd day, stopping the germination on the 6 th day, and calculating the germination rate. 20 cucumber seedlings (3 replicates) were selected for each treatment and the full length of the radicle (from the bottom of the radicle to the top of the hypocotyl) was measured; the surface moisture of the cucumber seeds is sucked dry by absorbent paper, and the fresh mass is weighed.
Germination percentage (%) — number of germinated seeds/number of test seeds × 100;
germination potential (%) -72 h germination seed number/test seed number × 100;
germination Index (GI) ═ Σ Gt/Dt (Gt denotes the number of sprouts td, Dt denotes the number of days to sprout);
viability Index (VI) ═ GI × S (GI means germination index, S means average root length).
The application of the liquid foliar fertilizer can promote the germination and growth of cucumber and tomato seeds, the cucumber sprout activity index is increased to 22.36%, the sprout root length is increased to 21.60%, and the sprout fresh weight is increased to 41.05% after diluting 100 times and soaking for 1 day, as shown in table 1, figure 1 and figure 2; the tomato plumule activity index after 1 day of seed soaking is increased to 14.32%, the plumule length of the plumule is increased to 15.92%, see table 2 and figure 3.
TABLE 1 Polyphenol waste liquid influence on cucumber seed Germination
TABLE 2 Polyphenol waste liquid Effect on tomato seed Germination
Example 2
The application of the polyphenol waste liquid generated by extracting fucoxanthin from brown algae in preparing the plant stress-resistant foliar fertilizer specifically comprises the following steps:
1) treating the polyphenol waste liquid: diluting the polyphenol waste liquid with clear water according to the proportion of 1:49 and 1: 99;
2) and (3) treating cucumber and tomato seeds: soaking seeds of cucumber and tomato seeds by using 1 wt% of fucoxanthin production waste liquid for 24 hours at room temperature (25 ℃), and then sowing;
3) and (3) treating cucumber seedlings and tomato seedlings in cotyledon stage: after flattening cucumber and tomato cotyledons, spraying 3-4ml of fucoxanthin production waste liquid with 2 wt% to the whole plant;
4) and (3) carrying out temperature stress treatment on cucumber and tomato seedlings: after flattening the cotyledons of the cucumbers and the tomato (respectively culturing for 4 days and 10 days), continuously culturing the seedlings of the cucumbers and the tomatoes in the environments of 4 ℃ (low temperature), 25 ℃ (room temperature) and 42 ℃ (high temperature), and counting the withered number of the seedlings every 24 hours until the seedlings are completely withered.
The application of the liquid foliar fertilizer can promote the tolerance of the cucumber and tomato cotyledon stage seedlings to the temperature stress, the tolerance of 1 wt% of cucumber seeds after seed soaking to the low temperature of 4 ℃ is improved by 8.33%, and the tolerance to the high temperature of 42 ℃ is improved by 19.99%; after 2 wt% of spraying, the tolerance of the cucumber seedlings to the low temperature of 4 ℃ is improved by 8.33%, and the tolerance to the high temperature of 42 ℃ is improved by 30.00%, which is shown in table 3 and figure 4; after 1% of tomato seeds are soaked, the tolerance to the low temperature of 4 ℃ is improved by 26.79%, and the tolerance to the high temperature of 42 ℃ is improved by 19.64%; after 2% spraying, the tolerance of the tomato seedlings to the low temperature of 4 ℃ is improved by 5.56%, and the tolerance to the high temperature of 42 ℃ is improved by 36.67%, which is shown in table 4 and figure 5.
TABLE 3 withering and death rates of cucumber seedlings in cotyledon period under different temperature treatment
TABLE 4 withering and death rates of tomato seedlings in cotyledon stage under different temperature treatment
TABLE 4 wither and death rates of tomato seedlings in cotyledon stage under different temperature treatment (continuous)
Example 3
The specific method for preparing the plant growth promoting foliar fertilizer by using the polyphenol waste liquid generated by extracting fucoxanthin from brown algae comprises the following steps:
1) treating the polyphenol waste liquid: before use, the polyphenol waste liquid and clean water are diluted according to the proportion of 1: 49;
2) and (3) treating cucumber seedlings in a leaf stage: spraying 10-15ml of polyphenol waste liquid per plant on the whole plant after third true leaves grow on the cucumber seedlings;
the application of the liquid foliar fertilizer can promote the growth of cucumber seedlings, and the plant height of the cucumber seedlings reaches 26.46% after the cucumber seedlings are used for 14 days, which is shown in table 5 and fig. 6.
TABLE 5 growth promoting effect of polyphenol waste liquid foliar fertilizer with different concentrations on cucumber seedlings in 3-leaf stage
Compared with the prior art, the brown algae-based fucoxanthin waste liquid contains biological stimulin such as brown algae polyphenol, brown algae oligosaccharide, jasmonic acid, abscisic acid, gibberellin, quercetin, hydrogen safflower bean acid, myricetin, catechol and the like, and has remarkable growth promotion effect on stress resistance and growth of cucumber seedlings in a proper concentration range. Therefore, the waste is prepared into the liquid fertilizer for inducing the stress resistance of the plants, a new technology of zero waste discharge and full resource utilization can be provided for the green production of the brown algae industry, the technology has environmental protection significance and high-efficiency agricultural economic value, is a new technology for guaranteeing the stress-resistant and safe production of crops, and is a technology urgently needed by related enterprises at present. Has wide application prospect.
1. The invention has obvious improvement on the temperature stress tolerance of the cucumber seedling in the cotyledon period, improves the tolerance of the cucumber seedling at the high temperature of 42 ℃ by 36.67 percent, and improves the tolerance of the cucumber seedling at the low temperature of 4 ℃ by 26.79 percent.
2. The invention has obvious growth promotion effect on cucumber seedlings in 3-leaf stage, and the plant height is improved by 26.46 percent after 14 days of use.
3. The method is beneficial to clean production and resource full utilization of fucoxanthin extracted from brown algae, and 10000 tons of polyphenol waste liquid can be generated when 50% fucoxanthin is produced by 1 ton per ton, so that the method has extremely wide application market.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A resource utilization method of polyphenol waste liquid of fucoxanthin extracted from brown algae is characterized by comprising the following steps: the polyphenol waste liquid is used for preparing plant stress-resistant foliar fertilizer, the polyphenol waste liquid is used for plant stress-resistant seed soaking liquid, the polyphenol waste liquid is used for preparing plant growth-promoting foliar fertilizer, and the polyphenol waste liquid is used for preparing liquid foliar fertilizer.
2. The resource utilization method of the polyphenol waste liquid from the brown algae extraction of the fucoxanthin according to the claim 1, wherein the application of the polyphenol waste liquid to the preparation of the plant stress-resistant foliar fertilizer specifically comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2, treating cotyledon cucumber seedlings: after the cucumber cotyledon is flattened, the whole plant is sprayed with 3-4mL of phenol waste liquid per plant.
3. The resource utilization method of the polyphenol waste liquid from the brown algae extraction of the fucoxanthin according to the claim 1, wherein the specific method for applying the polyphenol waste liquid to the plant stress-resistant seed soaking liquid comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 95-99;
s2, treating plant seeds: and (3) soaking the seeds of the plants by using the polyphenol waste liquid for 24 hours at room temperature, and then sowing the seeds.
4. The resource utilization method of the polyphenol waste liquid obtained by extracting fucoxanthin from brown algae according to claim 3, wherein the room temperature is 20-25 ℃.
5. The resource utilization method of the polyphenol waste liquid obtained by extracting fucoxanthin from brown algae according to claim 3, wherein the plant seeds are cucumber seeds or tomato seeds.
6. The resource utilization method of the polyphenol waste liquid from the brown algae extraction of the fucoxanthin according to the claim 1, wherein the specific method for preparing the plant growth promoting foliar fertilizer by using the polyphenol waste liquid comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2.3, treating the plant seedlings at the leaf stage: after the third true leaf of the plant seedling grows, spraying 10-15ml of polyphenol waste liquid per plant on the whole plant.
7. The resource utilization method of the polyphenol waste liquid from the brown algae extraction of the fucoxanthin of claim 6, wherein the plant seedlings are cucumber seedlings or tomato seedlings.
8. The resource utilization method of the polyphenol waste liquid from the brown algae extraction of the fucoxanthin according to the claim 1, wherein the specific method for using the polyphenol waste liquid to prepare the liquid foliar fertilizer comprises the following steps:
s1, treating polyphenol waste liquid: diluting the polyphenol waste liquid of the brown algae for extracting fucoxanthin with clear water according to the proportion of 1: 45-49;
s2.3, treating cucumber seedlings in leaf stage: spraying 10-15ml of polyphenol waste liquid per plant on the whole plant after the third true leaf of the cucumber seedling grows.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110479222.6A CN113197225A (en) | 2021-04-30 | 2021-04-30 | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110479222.6A CN113197225A (en) | 2021-04-30 | 2021-04-30 | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113197225A true CN113197225A (en) | 2021-08-03 |
Family
ID=77028244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110479222.6A Pending CN113197225A (en) | 2021-04-30 | 2021-04-30 | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113197225A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117137941A (en) * | 2023-09-20 | 2023-12-01 | 威海海高园明睿智琳生物科技有限公司 | A radiation-proof seaweed extract composition |
| CN118125877A (en) * | 2024-03-06 | 2024-06-04 | 烟台凡西农业科技有限公司 | Alginate oligosaccharide plant stress-resistant auxin, preparation method and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2460710A1 (en) * | 1979-04-02 | 1981-01-30 | Basset Francois | Absorbent prodn. contg. vegetable prod., pref. of coral family - used to absorb liquid and semi-liquid wastes |
| CN104412975A (en) * | 2013-09-02 | 2015-03-18 | 蒋华 | Preparation method for promoting crop growth by reducing organophosphorous residues |
| CN106396958A (en) * | 2016-09-09 | 2017-02-15 | 韩明三 | Preparation method and application of compound preparation for controlling physiological diseases of apples |
| CN107868767A (en) * | 2017-12-14 | 2018-04-03 | 宁波大学 | A kind of purple laver alga border probiotics and its application |
| CN108034008A (en) * | 2017-12-21 | 2018-05-15 | 大连深蓝肽科技研发有限公司 | A kind of method of comprehensive extraction algal polysaccharides, seeweed polyphenol and fucoxanthine |
| CN108617698A (en) * | 2018-06-05 | 2018-10-09 | 陈奥陶 | A kind of Sargassum horneri extract for promoting seed to sprout |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN111675555A (en) * | 2020-06-17 | 2020-09-18 | 秦山 | Application of anabaena and/or spirulina extract as biostimulant in agricultural production |
-
2021
- 2021-04-30 CN CN202110479222.6A patent/CN113197225A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2460710A1 (en) * | 1979-04-02 | 1981-01-30 | Basset Francois | Absorbent prodn. contg. vegetable prod., pref. of coral family - used to absorb liquid and semi-liquid wastes |
| CN104412975A (en) * | 2013-09-02 | 2015-03-18 | 蒋华 | Preparation method for promoting crop growth by reducing organophosphorous residues |
| CN106396958A (en) * | 2016-09-09 | 2017-02-15 | 韩明三 | Preparation method and application of compound preparation for controlling physiological diseases of apples |
| CN107868767A (en) * | 2017-12-14 | 2018-04-03 | 宁波大学 | A kind of purple laver alga border probiotics and its application |
| CN108034008A (en) * | 2017-12-21 | 2018-05-15 | 大连深蓝肽科技研发有限公司 | A kind of method of comprehensive extraction algal polysaccharides, seeweed polyphenol and fucoxanthine |
| CN108617698A (en) * | 2018-06-05 | 2018-10-09 | 陈奥陶 | A kind of Sargassum horneri extract for promoting seed to sprout |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN111675555A (en) * | 2020-06-17 | 2020-09-18 | 秦山 | Application of anabaena and/or spirulina extract as biostimulant in agricultural production |
Non-Patent Citations (2)
| Title |
|---|
| 主编：秦益民: "《功能性海藻肥》", 31 March 2019, 中国轻工业出版社 * |
| 刘志媛 等: "《海藻生物技术》", 31 July 2015, 20150731 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117137941A (en) * | 2023-09-20 | 2023-12-01 | 威海海高园明睿智琳生物科技有限公司 | A radiation-proof seaweed extract composition |
| CN118125877A (en) * | 2024-03-06 | 2024-06-04 | 烟台凡西农业科技有限公司 | Alginate oligosaccharide plant stress-resistant auxin, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103621289B (en) | Soilless culture method for tomatoes | |
| CN106857141B (en) | Method and reagent set for preventing and treating citrus yellow shoot | |
| CN113197225A (en) | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae | |
| CN114668010B (en) | Pollen polysaccharide extract and application thereof in promoting plant growth | |
| CN112573973A (en) | Seaweed enzymolysis seedling raising fertilizer, and preparation method and application thereof | |
| CN111320507A (en) | Functional liquid fertilizer, preparation method thereof and cotton fertilization method | |
| CN113603521A (en) | Soilless culture nutrient solution suitable for tea tree cuttage and artificial light indoor planting | |
| CN116806850A (en) | Plant growth promoter prepared from fungal metabolite | |
| CN108770865B (en) | Alba elymus nutans seed coating agent | |
| CN116636350A (en) | Garlic fruit mixed seedling raising method | |
| CN114208848B (en) | Corn growth regulator containing chitosan and preparation and use methods thereof | |
| Gatan et al. | Effect of radiation-modified kappa-carrageenan on the morpho-agronomic characteristics of mungbean [Vigna radiata (L.) R. Wilczek] | |
| CN114375640A (en) | Method for promoting growth of camellia oleifera seedlings by using dark-color endophytic fungi | |
| CN103931452A (en) | Filbert high-yield cultivation method | |
| CN111484376A (en) | Humic acid bio-organic fertilizer for potted chives and preparation method thereof | |
| CN105284878A (en) | Cotton defoliation ripening agent | |
| CN111718223A (en) | Special fertilizer for pear trees | |
| CN112047784A (en) | Tea-seed-cake-based compound pesticide fertilizer and preparation method thereof | |
| CN116458357B (en) | Method for promoting growth of fruit trees by rhizosphere soil microorganisms | |
| CN117643299B (en) | Method for improving salt tolerance of rice in bud phase and seedling phase by using 2-methoxybenzoic acid | |
| CN114451098B (en) | Method for promoting germination of pepper seeds | |
| CN112939677B (en) | Functional fertilizer and preparation process thereof | |
| CN117431193B (en) | New strain CD1 of pseudomonas as well as growth promoter and application thereof | |
| CN108157182B (en) | Culture method for reducing browning rate of apple explants | |
| CN111919694B (en) | Hydroponic solution and cultivation method of high-quality radix astragali thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210803 |
|
| RJ01 | Rejection of invention patent application after publication |