CN108617698A - A kind of Sargassum horneri extract for promoting seed to sprout - Google Patents
A kind of Sargassum horneri extract for promoting seed to sprout Download PDFInfo
- Publication number
- CN108617698A CN108617698A CN201810570945.5A CN201810570945A CN108617698A CN 108617698 A CN108617698 A CN 108617698A CN 201810570945 A CN201810570945 A CN 201810570945A CN 108617698 A CN108617698 A CN 108617698A
- Authority
- CN
- China
- Prior art keywords
- sargassum horneri
- extract
- concentrated
- sargassum
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Soil Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The object of the present invention is to provide a kind of Sargassum horneri extract that promotion seed is sprouted and its applications, the Sargassum horneri extract provided, preparation method is after winning the air bag of fresh Sargassum horneri, it cleans and blends, add methanol aqueous solvent, after extracting at ambient temperature, certain determining alcohol is concentrated in vacuo under 25 35 DEG C of cryogenic conditions for the first time, and stand still for crystals；Remaining solution is concentrated in vacuo to certain determining alcohol for the second time again, and stands still for crystals；Last water phase is concentrated to give Sargassum horneri extract.The Sargassum horneri air bag extract of the present invention is a kind of environmental-friendly, safe biological agent.The sprouting for promoting vegetable seeds using the extract of the present invention, using extensive.Moreover, the present invention is the comprehensive utilization of the Sargassum horneri to being endangered currently as algae tide.
Description
Technical field
The invention belongs to marine algae extract applied technical fields, and in particular to a kind of Sargassum horneri for promoting seed to sprout extraction
Object.
Background technology
In recent years, as global climate, environment run down, the problems such as all kinds of pest and disease damages, arid, high and low temperature stresses day
Become serious, and influence the growth and development of crops, the yield and quality of crop is made to decline.But now peasants use it is big
Mostly it is chemical fertilizer, chemical fertilizer do prodigious harm to the body of people, can also break ring soil.Many countries, which start to advocate energetically, to apply
With organic fertilizer, bio-fertilizer, develop green agriculture.Currently, application of the alga fertilizer in planting industry and aquaculture is in many countries
It is applied.South Africa, Britain and New Zealand are that production alga fertilizer is more successfully national, they produce the sea of upper kiloton every year
Algae fertilizer.On the basis of same kind of products at abroad is studied, China's research and development marine algae extract fertilizer/pesticide is significant, when in
State's marine algae resource is very abundant, the macro of existing spontaneous growth, and has the seaweed of artificial breeding, and raw material is inexhaustible.Two
Be China it is large agricultural country, crop varieties are abundant, and region is wide, there is very big market potential and application prospect.Third, sea
Contain abundant carbohydrate, nitrogenous compound, lipoid substance, phenolic compound and vitamin and inorganic constituents in algae
Deng having potential functional activity outstanding, extract to belong to the plant growth regulator of the environmental protection of pure natural green.
Seaweed is the plant lived in ocean, and shapes and sizes are quite different.Wherein macro is according to their face
Color is different, mainly there is red algae, green alga and brown alga.For example red algae has seaweed, agar etc.；Green alga has Enteromorpha, sea lettuce etc.；Brown alga has
Kelp, sargassum fusifome etc..Have many nutriments in seaweed, there is protein, fat, sugar, also each Plant Hormone, minerals and
Trace element, they are not only the nutrition that people like and plants grow required nutrition, and plant can be allowed to grow more
High, stratification, the fruit bigger of knot, more sweet tea, and be conducive to the maintenance of soil, it is more advantageous to the health of human body.
Currently, in the world manufacturer's industry alga fertilizer when the raw material of marine algae extract that uses is most common has：Kelp, horse hair
Algae, kelp, yellow tang and bulk kelp etc..If the product K elpak66 raw materials used in South Africa are exactly the elder brother acquired from West Coast South Africa
Cloth, it is the yellow tang in the Arctic Ocean that Norway Ai Er, which sows the raw material that marine algae extract Algea is used, and Qingdao bright moon alga fertilizer uses
Raw material be Shandong Peninsula coastal area kelp.
In recent years, China coast broke out Sargassum horneri disaster every year, and Sargassum horneri is in annual spring in one band of Zhejiang, Jiangsu and Shandong
Raised growth reaches into hundred tons up to ten thousand, not only influences littoral port security, also influences the cultivation of coastal economic algae.
It if it is possible to make full use of these Sargassum horneris, can help to alleviate ecological pressure, they can also be made product, generate
Certain economic value.
Invention content
The object of the present invention is to provide a kind of Sargassum horneri extract that promotion seed is sprouted and its applications, to reach abundant profit
With Sargassum horneri, and improve the purpose for the volume increase that gets agricultural profit.
Present invention firstly provides a kind of Sargassum horneri extract, preparation method is cleaning after winning the air bag of fresh Sargassum horneri
And blend, methanol aqueous solvent is added, after extracting at ambient temperature, is concentrated in vacuo for the first time under 25-35 DEG C of cryogenic conditions
To certain determining alcohol, and stand still for crystals；Remaining solution is concentrated in vacuo to certain determining alcohol for the second time again, and stands still for crystals；Most
Water phase afterwards is concentrated to give Sargassum horneri extract；
Preferably, the volume ratio of the methanol and water in methanol aqueous extract is 5:1~4:1；
Preferably, the mass volume ratio g/ml of Sargassum horneri air bag and extracting solution is 1:3~1:5；
Preferably, being concentrated in vacuo to determining alcohol in 60%-65% for the first time；
Preferably, being concentrated in vacuo to determining alcohol for the second time in 30%-45%；
Another aspect of the present invention provides the application of Sargassum horneri extract, is the application in promoting seed to sprout.
It is in the seed soaking phase the present invention also provides a kind of method that promotion seed is sprouted, seed is soaked in a concentration of 50~
30min~1h in the Sargassum horneri extracting solution of 200 μ g/ml, then plant in soil.
The Sargassum horneri air bag extract of the present invention is a kind of environmental-friendly, safe biological agent.Use carrying for the present invention
Object is taken to promote the sprouting of vegetable seeds, using extensive.Moreover, the present invention is the comprehensive profit of the Sargassum horneri to being endangered currently as algae tide
With.
Description of the drawings
Fig. 1：Sprouting and growing state of the Sargassum horneri air bag extract to cucumber seeds, wherein 1. clear water groups；2. common fertilizer
Group；3-5 is the group of 50,100,200 μ g/ml extracts of addition respectively；
Fig. 2：Influence diagram of the Sargassum horneri extract to plant height.
Specific implementation mode
Below in conjunction with specific embodiment, present invention is further described in detail.
Embodiment 1：
After applicant first grinds Sargassum horneri different parts, with methanol-water-formic acid (15:4:1, v/v/v) it extracts,
Extracting solution is sprouted with cucumber seeds, each organizes 6 seeds, after seed uses the extracting solution of different parts to impregnate 30min respectively,
One is put into sprout in bag.Sargassum horneri extract solution is continuously added in bag, and using clear water as negative control group.As a result, it has been found that gas
Capsule position is best to the sprouting effect of cucumber seeds, and just there is 1 germination in when 48h, and in 60h, germination percentage reaches 4,
All it is higher than the extract (table 1) at other two Sargassum horneri positions；Therefore, select Sargassum horneri air bag as the material of extraction.
Table 1：Contrast table of the Sargassum horneri different parts extract to percentage of seedgermination
| Clear water group | Blade | Air bag | Stem | |
| 48h | 0/6 | 0/6 | 1/6 | 0/6 |
| 60h | 2/6 | 2/6 | 4/6 | 3/6 |
| 72h | 5/6 | 6/6 | 6/6 | 6/6 |
2 extracting method of embodiment it is preferred
It is extracted first using the method reported for work：
Fresh Sargassum horneri is cleaned and is blended, and adds methanol aqueous solvent, after extracting at ambient temperature, 25-35 DEG C of low temperature
Under the conditions of be concentrated in vacuo obtain Sargassum horneri extract (extract 1 in table 2)；
The extract obtained using the above method carries out sprouting experiment to air bag extracting solution to cucumber seeds, as a result, it has been found that on
It states and needs high concentration in the extract of method extraction just and have good effect；And rooting efficiency is bad.
Above-mentioned in order to overcome the problems, such as, extracting method is optimized in the present invention.
The method that the present invention is established is as follows：
After fresh Sargassum horneri acquisition, cell parts 200g therein is detached, after being cleaned 3 times using clear water.It is beaten using pulper
It is 2-3mm to be broken to granularity, and methanol is added in press dewatering：Water=15:The solution 800ml of 4 (v/v), it is ultrasonic in dark at 25 DEG C
Extract 30min, filtering.By leaching liquor at 30 DEG C cryogenic vacuum be concentrated into concentration of alcohol be 60%, standing wait for chlorophyll precipitate
Completely, the filtering removal substances such as chlorophyll, then cryogenic vacuum is concentrated into concentration of alcohol is 35% at 30 DEG C, stands to be crystallized complete
Entirely, the substances such as filtering removal fucoxanthin, 20ml is concentrated by solution cryogenic vacuum, dry, the low-temperature storage (extraction in table 2
Object 2).
The Sargassum horneri extract of the two methods extraction of the present embodiment is detected, steps are as follows：
By the extract of two methods respectively with the methanol-water-acetic acid (90 of 1mL ammonium acetates containing 10mmol/L:10:0.05,
V/v/v it) redissolves, liquid 10000r/min at 4 DEG C will be redissolved and centrifuge 10min, take supernatant through 0.22 μm of organic membrane filtration, on
LC-MS is analyzed.
Chromatographic condition：It is solid that Thermo Hypersil Gold C18 chromatographic columns (100mm × 2.1mm, 3 μm), which are respectively adopted,
Determine phase；Mobile phase is aqueous solution (A)-methanol (B) of the ammonium acetate containing 10mmol/L, and gradient is：The Mobile phase B in 10min
It is raised to 95% from 15%, 15% is dropped in 1min after keeping 1min, keeps 3min；Flow velocity is set as 0.3mL/min, column temperature 30
DEG C, 10 μ L of sample size.
Mass Spectrometry Conditions：Be respectively adopted electron spray ionisation source positive and negative ion ionization pattern, spray voltage be respectively 3kV and
2.5kV, sheath throughput 20L/min, secondary air amount 10L/min, 270 DEG C of ion transfer tube temperature, Q2 collision cell collision-induceds
Ionization voltage 1.5V is solved, using Selective reaction monitoring (SRM) scan pattern.
To the measurement result of extract show in extract containing heteroauxin, gibberellin, brassin Inner esters, zeatin,
The substances such as isopentennyladenine.
Table 2：The content detection table (ng/g, X ± SD) of the extract of Different Extraction Method
Embodiment 3：
Air bag extracting solution in above-mentioned case study on implementation 2 is diluted to 50,100,200 μ g/ml with water, chooses the cucumber of purchase
Totally 30, seed each organizes 6 seeds, after seed uses the extracting solution of various concentration to impregnate 30min respectively, is put into a sprouting bag
In.The Sargassum horneri extract solution of various concentration is continuously added in bag, and using clear water as negative control group, with the general of commodity purchasing
Logical Liquid Fertilizer is as positive control.
Cucumber seeds start rudiment on day 2, and the 3rd day starts apparent root occur.
1) influence of the Sargassum horneri extract to Seed Germination in Cucumber rate
In terms of the result of table 3, cucumber seeds germinateed since the 2nd day, wherein and the seed of clear water group does not germinate on day 2,
And commonly organize and also do not germinate, but Sargassum horneri extract starts to germinate, and in the 3rd group of hair of 200 most μ g/ml of additive amount
3 buds.When by the 3rd day, other than clear water group sends out 5 buds, all the buds are coming out for other groups of all seeds.Illustrate Sargassum horneri extract
There is facilitation to seed germination.
Table 3：Influence of the Sargassum horneri extract to percentage of seedgermination
| Clear water group | Common group | 50μg/ml | 100μg/ml | 200μg/ml | |
| 2nd day | 0/6 | 0/6 | 1/6 | 1/6 | 3/6 |
| 3rd day | 5/6 | 6/6 | 6/6 | 6/6 | 6/6 |
2) influence of the Sargassum horneri extract to cucumber root long
Cucumber was since the 3rd day, and germination when also has root long to come out, either which day, can find out plus common battalion
The root long of nutrient solution is longer than clear water group, and in the cucumber that Sargassum horneri extract-treated is crossed, 1 group few of effect of additive amount is bad, root long
Even it is not so good as clear water group, but after additive amount increase, just clearly, 2 and 3 groups of algae fertilizer is all than clear water group and ordinary nutritional for effect
Liquid group is high, and addition is more, and root long is longer (table 4).
Influence (mm) of the 4 Sargassum horneri extract of table to root long
| Clear water group | Common group | 50μg/ml | 100μg/ml | 200μg/ml | |
| 3rd day | 41.00 | 51.67 | 41.50 | 61.67 | 64.75 |
| 4th day | 62.20 | 60.17 | 47.17 | 86 | 88.75 |
| 5th day | 67.20 | 65.00 | 48.83 | 90.33 | 91.75 |
| 6th day | 90.80 | 91.67 | 71.33 | 112.83 | 119 |
| 7th day | 108.60 | 105.50 | 88.50 | 132.67 | 153.20 |
3) influence of the Sargassum horneri extract to cucumber plant height
Table 5 as a result, it has been found that, Sargassum horneri extract has preferable effect to the growth of cucumber, has added the cucumber seedling of Sargassum horneri extract
Growing height it is all higher than clear water group and ordinary nutritional liquid group, but not addition it is more, effect is better, but in additive amount
When intermediate, effect is best, and addition is excessive, and plant height reduces instead.
Influence (mm) of the 5 Sargassum horneri extract of table to plant height
| Clear water group | Common group | 50μg/ml | 100μg/ml | 200μg/ml | |
| 4th day | 45.00 | 41.80 | 45.00 | 47.00 | 48.60 |
| 5th day | 49.40 | 50.00 | 53.50 | 56.00 | 50.80 |
| 6th day | 56.20 | 56.83 | 60.67 | 63.83 | 59.33 |
| 7th day | 63.00 | 35.00 | 61.33 | 71.67 | 61 |
Embodiment 4：
2 extracting solution of above-mentioned case study on implementation is diluted to 100 μ g/ml with water, chooses the I.balsamina L.seeds totally 20 of purchase, point
For blank group (not impregnating) and sample sets, 10 seeds are each organized, after seed uses the extracting solution of various concentration to impregnate 1h respectively, kind
Enter in soil.
1) influence of the Sargassum horneri extract to I.balsamina L.seeds germination percentage
In terms of the result of table 6, I.balsamina L.seeds germinateed successively since the 4th day, wherein the seed of clear water group was at the 6th day
It is preceding not germinate, but Sargassum horneri extract germinateed since the 4th day.When by the 7th day, other than clear water group sends out 6 buds, sample
All the buds are coming out for all seeds of group.Illustrate that Sargassum horneri extract has facilitation to seed germination.
Table 6：Influence of the Sargassum horneri extract to percentage of seedgermination
| Clear water group | 100μg/ml | |
| 4th day | 0/10 | 1/10 |
| 5th day | 2/10 | 5/10 |
| 6th day | 6/10 | 8/10 |
| 7th day | 8/10 | 10/10 |
| 8th day | 10/10 | 10/10 |
Influence of the Sargassum horneri extract to balsamine plant height
As a result, it has been found that Sargassum horneri extract has preferable effect to the growth of balsamine, the balsamine of Sargassum horneri extract is added
The growing height of seedling is obviously (Fig. 2) higher than clear water group.
The above results show that the extract of the present invention can effectively facilitate seed sprouting, have good application prospect.
Claims (8)
1. a kind of Sargassum horneri extract, which is characterized in that the preparation method of the Sargassum horneri extract is by the air bag of fresh Sargassum horneri
It after winning, cleans and blends, add methanol aqueous solvent, after extracting at ambient temperature, first under 25-35 DEG C of cryogenic conditions
It is secondary to be concentrated in vacuo to certain methanol concentration, and stand still for crystals；Remaining solution is concentrated in vacuo to certain methanol concentration for the second time again,
And it stands still for crystals；Last water phase is concentrated to give Sargassum horneri extract.
2. Sargassum horneri extract as described in claim 1, which is characterized in that the body of methanol and water in the methanol aqueous solvent
Product is than being 5:1~4:1.
3. Sargassum horneri extract as described in claim 1, which is characterized in that the mass body of the Sargassum horneri air bag and alcohol aqueous solvent
Product is 1 than g/ml:3~1:5.
4. Sargassum horneri extract as described in claim 1, which is characterized in that the first time is concentrated in vacuo to methanol concentration and is
60%-65%.
5. Sargassum horneri extract as described in claim 1, which is characterized in that the methanol concentration that is concentrated in vacuo to for the second time is
30%-45%.
6. application of the Sargassum horneri extract described in claim 1 in promoting seed to sprout.
7. a kind of method for promoting seed to sprout, which is characterized in that the method is that seed is soaked in a concentration of 50~200
30min~1h in the Sargassum horneri extracting solution of μ g/ml, then plant in soil.
8. the method for claim 7, which is characterized in that the seed is cucumber or I.balsamina L.seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810570945.5A CN108617698A (en) | 2018-06-05 | 2018-06-05 | A kind of Sargassum horneri extract for promoting seed to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810570945.5A CN108617698A (en) | 2018-06-05 | 2018-06-05 | A kind of Sargassum horneri extract for promoting seed to sprout |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108617698A true CN108617698A (en) | 2018-10-09 |
Family
ID=63691276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810570945.5A Pending CN108617698A (en) | 2018-06-05 | 2018-06-05 | A kind of Sargassum horneri extract for promoting seed to sprout |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108617698A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601138A (en) * | 2018-12-11 | 2019-04-12 | 江苏丰收大地种业发展有限公司 | A kind of Seed Collecting Methods of vegetables wheatgrass |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN113197225A (en) * | 2021-04-30 | 2021-08-03 | 上海交通大学 | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae |
| CN114271467A (en) * | 2021-12-30 | 2022-04-05 | 广州百花香料股份有限公司 | A method for preparing gorgeous leaf extract and the prepared gorgeous leaf extract |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926998A (en) * | 2017-11-14 | 2018-04-20 | 张静 | A kind of preparation method of seed coat agent |
-
2018
- 2018-06-05 CN CN201810570945.5A patent/CN108617698A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926998A (en) * | 2017-11-14 | 2018-04-20 | 张静 | A kind of preparation method of seed coat agent |
Non-Patent Citations (2)
| Title |
|---|
| 武冬雪等: "铜藻提取液对几种中草药种子萌发的影响", 《种子》 * |
| 袁清香等: "铜藻Sargassu Horneri的化学成分研究", 《广东化工》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601138A (en) * | 2018-12-11 | 2019-04-12 | 江苏丰收大地种业发展有限公司 | A kind of Seed Collecting Methods of vegetables wheatgrass |
| CN110214794A (en) * | 2019-05-10 | 2019-09-10 | 海盐县凌特生物科技有限公司 | Accelerate the preparation method for the regulator that seed is sprouted |
| CN113197225A (en) * | 2021-04-30 | 2021-08-03 | 上海交通大学 | Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae |
| CN114271467A (en) * | 2021-12-30 | 2022-04-05 | 广州百花香料股份有限公司 | A method for preparing gorgeous leaf extract and the prepared gorgeous leaf extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108617698A (en) | A kind of Sargassum horneri extract for promoting seed to sprout | |
| CN101717287B (en) | Grape nutrition-balanced foliar fertilizer and preparation method thereof | |
| CN103392588A (en) | Kiwi fruit suspension liquid atomizing artificial pollination method | |
| CN105052452A (en) | Intercropping method of osmanthusfragrans trees, tea trees and ginkgo trees | |
| CN105801287A (en) | Water-soluble fertilizer containing amino acid and preparation method of water-soluble fertilizer | |
| CN107189785A (en) | The conditioning agent that Cranberry uses when being interplanted with Chinese yew | |
| CN103087975A (en) | Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity | |
| CN106106002A (en) | A kind of fruit Mulberry grafting is when the method for annual bearing | |
| CN113080056B (en) | Cultivation method of ginseng and Ji fruit | |
| CN104206139A (en) | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato | |
| CN104230404B (en) | A kind of preparation method for digesting high activity seaweed organic fertilizer material and products thereof | |
| CN105523791A (en) | Organic fertilizer for tea and preparation method of organic fertilizer | |
| CN109644820A (en) | A kind of nutrient soil that plantation sweet potato uses | |
| CN109422570A (en) | A kind of production method of Enoki mushroom cultivation material compatibility and this culture material | |
| CN107114415A (en) | Matching used conditioning agent and growth accelerator when Chinese yew is interplanted in vineyard | |
| Sun | Pineapple production and research in China | |
| Abdulkadhim et al. | Effectiveness of brassinolide and dry yeast extract spraying on growth parameters and the chemical content of the grape seedlings | |
| CN112640921A (en) | Rice cold-resistant agent and application thereof | |
| CN105393882A (en) | QuercusvariabilisBl container nursery method | |
| CN101597632A (en) | Mycelium protein powder making method and contain the paddy rice Coldproof protein fertilizer of this protein powder | |
| CN109691375A (en) | A kind of dendrobium officinale culture medium and its preparation method and application based on pecan shell | |
| CN104030747A (en) | Alkali organic fertilizer and preparation method thereof | |
| CN107935697A (en) | A kind of canarium pimela tree special fertilizer and preparation method thereof | |
| Zaifu | From shifting cultivation to agroforestry in the mountain areas of Yunnan tropics | |
| CN102696382A (en) | Indigowoad root suitable for sprout production and method for growing indigowoad root sprouts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181009 |