CN1130971C - 制备用作细胞种植框架和植入体的生物细胞外基质的方法 - Google Patents
制备用作细胞种植框架和植入体的生物细胞外基质的方法 Download PDFInfo
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- CN1130971C CN1130971C CN00120211A CN00120211A CN1130971C CN 1130971 C CN1130971 C CN 1130971C CN 00120211 A CN00120211 A CN 00120211A CN 00120211 A CN00120211 A CN 00120211A CN 1130971 C CN1130971 C CN 1130971C
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- described method
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- tissue
- cell
- alkaline solution
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Links
- 239000007943 implant Substances 0.000 title description 2
- 238000004519 manufacturing process Methods 0.000 title 1
- 210000001519 tissue Anatomy 0.000 claims abstract description 43
- 210000004027 cell Anatomy 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 29
- 210000002744 extracellular matrix Anatomy 0.000 claims abstract description 19
- 241001465754 Metazoa Species 0.000 claims abstract description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 14
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 14
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000012670 alkaline solution Substances 0.000 claims description 12
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 210000004204 blood vessel Anatomy 0.000 claims description 6
- 210000000845 cartilage Anatomy 0.000 claims description 6
- 235000001014 amino acid Nutrition 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 5
- 239000000834 fixative Substances 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 4
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 3
- 235000013922 glutamic acid Nutrition 0.000 claims description 3
- 239000004220 glutamic acid Substances 0.000 claims description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 239000000908 ammonium hydroxide Substances 0.000 claims description 2
- 230000012447 hatching Effects 0.000 claims description 2
- 210000003491 skin Anatomy 0.000 claims 3
- 230000007935 neutral effect Effects 0.000 claims 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 1
- 238000002054 transplantation Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 210000003850 cellular structure Anatomy 0.000 abstract 1
- 238000005406 washing Methods 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
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- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
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- 239000000758 substrate Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- MNEIPOIFQCXXGM-UHFFFAOYSA-M O.O.O.[OH-].[Na+] Chemical compound O.O.O.[OH-].[Na+] MNEIPOIFQCXXGM-UHFFFAOYSA-M 0.000 description 1
- 229920001963 Synthetic biodegradable polymer Polymers 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- -1 asparatate Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003037 histogenic effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
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- Materials For Medical Uses (AREA)
Abstract
Description
Claims (20)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN00120211A CN1130971C (zh) | 2000-07-13 | 2000-07-13 | 制备用作细胞种植框架和植入体的生物细胞外基质的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN00120211A CN1130971C (zh) | 2000-07-13 | 2000-07-13 | 制备用作细胞种植框架和植入体的生物细胞外基质的方法 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN96104550A Division CN1061812C (zh) | 1996-04-01 | 1996-04-01 | 制备皮肤移植体的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1304655A CN1304655A (zh) | 2001-07-25 |
CN1130971C true CN1130971C (zh) | 2003-12-17 |
Family
ID=4588156
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN00120211A Expired - Lifetime CN1130971C (zh) | 2000-07-13 | 2000-07-13 | 制备用作细胞种植框架和植入体的生物细胞外基质的方法 |
Country Status (1)
Country | Link |
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CN (1) | CN1130971C (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2940146A4 (en) * | 2012-12-26 | 2016-08-10 | Quarrymen Corp | HIGH-FUNCTIONAL IMPLANT MATERIAL |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3649163A (en) * | 1968-03-25 | 1972-03-14 | American Cyanamid Co | Method for removing noncollagenous matter from mammalian gut |
EP0128706A2 (en) * | 1983-06-10 | 1984-12-19 | University Patents, Inc. | Body implants of extracellular matrix and means and methods of making and using such implants |
EP0358506A2 (en) * | 1988-09-08 | 1990-03-14 | MARROW-TECH INCORPORATED (a Delaware corporation) | Three-dimensional cell and tissue culture system |
-
2000
- 2000-07-13 CN CN00120211A patent/CN1130971C/zh not_active Expired - Lifetime
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3649163A (en) * | 1968-03-25 | 1972-03-14 | American Cyanamid Co | Method for removing noncollagenous matter from mammalian gut |
EP0128706A2 (en) * | 1983-06-10 | 1984-12-19 | University Patents, Inc. | Body implants of extracellular matrix and means and methods of making and using such implants |
US4801299A (en) * | 1983-06-10 | 1989-01-31 | University Patents, Inc. | Body implants of extracellular matrix and means and methods of making and using such implants |
EP0358506A2 (en) * | 1988-09-08 | 1990-03-14 | MARROW-TECH INCORPORATED (a Delaware corporation) | Three-dimensional cell and tissue culture system |
Also Published As
Publication number | Publication date |
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CN1304655A (zh) | 2001-07-25 |
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