CN112715552A

CN112715552A - Bactericidal composition containing propionyl brassinolide and polyoxin and application thereof

Info

Publication number: CN112715552A
Application number: CN202110096142.2A
Authority: CN
Inventors: 刘言合; 陈琦
Original assignee: Heilongjiang Juzhuo Crop Science Co ltd
Current assignee: Heilongjiang Juzhuo Crop Science Co ltd
Priority date: 2021-01-25
Filing date: 2021-01-25
Publication date: 2021-04-30

Abstract

The invention discloses a bactericidal composition containing propionyl brassinolide and polyoxin, which comprises agriculturally acceptable active ingredients, an auxiliary agent, a carrier or/and a solvent, wherein the active ingredients comprise propionyl brassinolide and polyoxin, the content of the propionyl brassinolide is 0.0015-0.0500%, and the content of the polyoxin is 1% -20%. The bactericidal composition and the application thereof can effectively prevent and treat rice sheath blight disease, can effectively reduce the dosage of polyoxin, delay the generation of resistance, shorten the safe interval period of medication, increase the crop yield, improve the crop quality, improve the stress resistance of rice, increase the capabilities of disease resistance, drought resistance, waterlogging resistance, high temperature resistance and the like, improve the capability of self-generation of rice in resisting adverse environments, and have strong economic and social values.

Description

Bactericidal composition containing propionyl brassinolide and polyoxin and application thereof

Technical Field

The invention relates to the field of pesticide sterilization compositions and application thereof, in particular to a sterilization composition containing propionyl brassinolide and polyoxin and application thereof.

Background

The brassinolide compound is a novel green environment-friendly plant growth regulator, can promote the growth of crops such as vegetables, melons, fruits and the like through seed soaking with proper concentration and stem and leaf spraying treatment, can improve the quality of the crops, can improve the yield of the crops, can also improve the drought resistance and cold resistance of the crops, and can relieve the symptoms of plant diseases and insect pests, phytotoxicity, fertilizer injury and freezing injury of the crops. The brassinolide compound is a plant endogenous hormone, has high biological activity, widely exists in organs such as pollen, seeds, stems, leaves and the like of plants, is harmless to human and livestock, and has safe and effective normal dosage.

Polyoxin (CAS number: 19396-03-3) is a metabolite produced by streptomyces aureus, and belongs to a broad-spectrum antibiotic bactericide. Has better internal absorption and conduction effects. The action mechanism is to interfere the biosynthesis of chitin on the cell wall of the pathogenic bacteria, so that the cell wall of the bacteria cannot carry out biosynthesis and the pathogenic bacteria die. After the germ tube and hyphae contact the medicament, the local part expands, breaks and overflows the cell contents, and the cell cannot normally develop, so that the cell dies. Therefore, the composition also has the effects of inhibiting pathogenic bacteria from sporulating and disease spot from expanding. Has better control effect on cucumber downy mildew, powdery mildew, ginseng black spot, apple and pear gray spot and rice sheath blight.

Application of chemical agents is the most effective means of controlling plant diseases. However, continuous application of a single chemical fungicide in a high dose for a long period of time is likely to cause problems such as drug residue, environmental pollution, and development of drug-resistant fungi. The reasonable compounding or mixing of the chemical bactericide has the positive characteristics of expanding the bactericidal spectrum, improving the control effect, prolonging the application suitable period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the occurrence and the development of the drug resistance and the like of fungi, and the compounding of the bactericide is one of the most effective methods for solving the problems. The price of developing new products of bactericides is continuously rising, and compared with the price of developing and researching high-efficiency, low-toxicity and low-residue compounding and mixing, the bactericide has the advantages of low investment, short development period, attention paid to at home and abroad, and development strength are increased in many cases. The propionyl brassinolide and polyoxin are screened out for compounding on the basis of indoor screening and field experiments, and the synergy is obvious. And the propionyl brassinolide and polyoxin compounded sterilization composition and the application thereof are not reported at present.

Disclosure of Invention

The invention aims to solve the technical problems in the prior art and provides a propionyl brassinolide and polyoxin-containing bactericidal composition and application thereof.

In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:

the sterilizing composition comprises agriculturally acceptable active ingredients, an auxiliary agent, a carrier or/and a solvent, wherein the active ingredients comprise propionyl brassinolide and polyoxin, the content of the propionyl brassinolide is 0.0015-0.0500%, and the content of the polyoxin is 1% -20%.

The content of the propionyl brassinolide is 0.0015%.

The content of the polyoxin is 15%.

The dosage form of the bactericidal composition is one of water aqua, soluble powder, wettable powder and suspending agent.

An application of propionyl brassinolide and polyoxin in preventing and treating the diseases of crops is disclosed.

The crops are rice.

The disease is banded sclerotial blight.

Has the advantages that:

the propionyl brassinolide and polyoxin-containing bactericidal composition and the application thereof provided by the invention can effectively prevent and treat rice sheath blight, can effectively reduce the dosage of polyoxin, delay the generation of resistance, shorten the medication safety interval, increase the crop yield, improve the crop quality, improve the rice stress resistance, increase the capabilities of disease resistance, drought resistance, waterlogging resistance, high temperature resistance and the like, and improve the capability of self-generation of rice for resisting adverse environments, and the bactericidal composition has strong economic and social values.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described with the following embodiments, but the present invention is by no means limited to these examples.

Example 1: propionyl brassinolide 0.001% and polyoxin 25%;

example 2: 0.0015% propionyl brassinolide 20% polyoxin;

example 3: 0.0015% propionyl brassinolide + 15% polyoxin;

example 4: 0.0015% propionyl brassinolide + 10% polyoxin;

example 5: 0.0015% propionyl brassinolide + 5% polyoxin;

example 6: 0.0030% propionyl brassinolide + 15% polyoxin.

Comparative example 1: 0.0100% propionyl brassinolide;

comparative example 2: 25% polyoxin;

comparative example 3: and (4) clear water.

Test experiments

1. Test strain and culture thereof

The rice sheath blight strain (Rhizoctonia solasonii) was provided by the central laboratory of the department of natural pesticide and chemical and biological education of southern agricultural university. The strain is stored at 4 ℃ for a long time, before activity determination, a test strain needs to be subjected to activation culture on a PDA (personal digital assistant) flat plate for 5-10 days, and then subculture is carried out for 1-2 times, so that the method can be used for activity determination.

2. Determination of fungal growth inhibition Activity

The method for the plate assay with toxicity was described in Quiroga et al. The pesticide compositions prepared in examples 1 to 6 and comparative examples 1 to 3 were used as test samples, and the test samples were prepared into mother solutions of a predetermined concentration by using solvents (ethanol was used as a solvent). Then, a certain amount of mother liquor (i.e. 0.5mL of mother liquor in 50mLPDA culture medium) is taken and mixed in PDA culture medium (50-60 ℃) fully, so that the concentration of the test sample in the culture solution is 0.1 mg/L. And uniformly pouring the mixed culture medium into a culture dish, and cooling and solidifying the culture medium to obtain the culture medium with the toxicity. The prepared cake was transplanted to a medium with virus using a sterilized inoculating needle or forceps, and 1 cake was inoculated to the center of each dish. The experiment was repeated 3 times for each treatment with a negative control (solvent instead of test sample), a blank control (water instead of test sample).

3. Result observation and data processing

And (3) putting each treatment into a constant-temperature incubator at 28 ℃ for culture, observing the result by using a toxic plate method when the blank control bacterial colony approaches the edge of a culture dish, and measuring the diameter of the bacterial colony by using a cross method. The inhibition rate was calculated using the following formula:

measuring the colony spread diameter (mm) -the average spread diameter (mm) of the colony-the cake diameter (mm);

relative inhibition% ((negative control colony expansion diameter-treated colony expansion diameter)/negative control expansion diameter × 100%).

TABLE 1

The rice susceptibility grade evaluation adopts a 0-9 grade weighing method of the International Rice research institute. The calculation of the test method, the infection degree and the control effect (14 days after application) of the rice sheath blight disease in the field is shown in the method of the thesis "Activity and efficacy of Bacillus subtilis strain NJ-18 againt force sheath and sclerotium rot of tape". The control effect of each test sample is shown in table 2.

Experimental group	Control effect (14 days after pesticide application)
		Example 1	95.23％
Example 2	94.66％
		Example 3	94.37％
Example 4	92.19％
		Example 5	87.95％
Example 6	94.12％
		Comparative example 1	/
Comparative example 2	85.34
		Comparative example 3	/

As shown in Table 2, the propionyl brassinolide and the polyoxin are compounded, so that the effect of the pesticide composition on preventing rice sheath blight disease is obviously improved; as can be seen from comparative examples 1-6, the most significant synergy is achieved when the contents of propionyl brassinolide and polyoxin are 0.0015% and 15%, respectively.

The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.

Claims

1. A bactericidal composition containing propionyl brassinolide and polyoxin is characterized in that: the bactericidal composition comprises agriculturally acceptable active ingredients, an auxiliary agent, a carrier or/and a solvent, wherein the active ingredients comprise propionyl brassinolide and polyoxin, the content of the propionyl brassinolide is 0.0015-0.0500%, and the content of the polyoxin is 1% -20%.

2. The propionyl brassinolide and polyoxin-containing bactericidal composition according to claim 1, wherein: the content of the propionyl brassinolide is 0.0015%.

3. The propionyl brassinolide and polyoxin-containing bactericidal composition according to claim 2, wherein: the content of the polyoxin is 15%.

4. The propionyl brassinolide and polyoxin-containing bactericidal composition according to claim 1, wherein: the dosage form of the bactericidal composition is one of water aqua, soluble powder, wettable powder and suspending agent.

5. Use of a propionyl-containing brassinolide and polyoxin as claimed in any one of claims 1 to 4 for the control of crop diseases.

6. The use of a propionyl-containing brassinolide and polyoxin for the control of crop pathogens according to claim 5, wherein: the crops are rice.

7. The use of a propionyl-containing brassinolide and polyoxin for the control of crop pathogens according to claim 6, wherein: the disease is banded sclerotial blight.