CN107333783A - Application of the composition containing methyl jasmonate in citrus bacterial canker disease - Google Patents
Application of the composition containing methyl jasmonate in citrus bacterial canker disease Download PDFInfo
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- CN107333783A CN107333783A CN201710429983.4A CN201710429983A CN107333783A CN 107333783 A CN107333783 A CN 107333783A CN 201710429983 A CN201710429983 A CN 201710429983A CN 107333783 A CN107333783 A CN 107333783A
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- Prior art keywords
- zhongshengmycin
- methyl jasmonate
- composition
- composition containing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
Abstract
The present invention relates to the composition containing methyl jasmonate citrus bacterial canker disease application.Described composition active ingredient is made up of Zhongshengmycin and methyl jasmonate, and the weight ratio of Zhongshengmycin and methyl jasmonate is 3 20:1.The present composition is compounded Zhongshengmycin and methyl jasmonate according to specific weight ratio, with obvious synergistic function, drug effect can be significantly improved, advantageously reduce each single dose usage amount, delay resistance of the germ to Zhongshengmycin, extend the life cycle of antibioticses agricultural chemicals Zhongshengmycin.
Description
Technical field
The present invention relates to disinfectant use in agriculture application field, more particularly to the composition containing methyl jasmonate in citrus bacterial canker disease
Application.
Background technology
In recent years, the Citrus Cultivars planted with Guangxi increase, and the generation of canker is also more and more common, c itrus canker
Sick prophylactico-therapeutic measures has a lot, and chemical prevention is important measures.Copper agent prevention effect is preferable in the medicament of existing chemical prevention, but holds
Easily induce mite evil and be also easy to produce poisoning, influence fruit quality.Therefore, efficient, less toxic, safe citrus bacterial canker disease preventing and treating medicine is sought
Agent is particularly important.
Zhongshengmycin, English name are：Zhongshengmycin, is N- glucoside antibiotic bactericides, its antimicrobial spectrum
Extensively, being capable of resisting gram-positive, negative germ, mycobacteria, saccharomycete and filamentous fungi.Especially to crops pathogenic bacteria and in vain
Dish Bacteria erwinia, avenae subsp.citrull, rice leaf spot bacteria, bacterial leaf streak of rice, citrus bacterial canker disease etc. are respectively provided with bright
Aobvious antibacterial activity.
Methyl jasmonate (Methyl Jasmonate, JA_Me), it is the derivative of a kind of aliphatic acid, is present in high plant
Endogenous growth regulators matter in object, it is that a kind of plant inducer impedance disease research field applies wider high activity exciton,
Effect in terms of induction disease resistance of plant has enjoyed people to pay close attention to.Lot of experiments confirmation, Jasmonates (jasm onates, Jas)
As the signal of interest molecule of Plant defense responses, the defence protection mechanism related to Plant defense responses can be activated, induction is planted
Thing produces the systemic acquired resistance to multiple diseases.
In agricultural production, compounded by the active ingredient of the different mechanisms of action, be to delay disease generation resistance normal
Method, Zhongshengmycin is different from the methyl jasmonate mechanism of action, and mixture has good synergy in the range of certain proportion
Effect, it is used for citrus bacterial canker disease at home and abroad there is not yet relevant report on the mixture of Zhongshengmycin and methyl jasmonate at present.
The content of the invention
The technical problem to be solved in the present invention is to provide application of the composition containing methyl jasmonate in citrus bacterial canker disease,
The significant raising drug effect of energy, can effectively control the development of disease, and be advantageous to alleviate the germ resistance to the action of a drug.
In order to solve the above-mentioned technical problem, the present invention adopts the technical scheme that：Composition containing methyl jasmonate is in mandarin orange
The application of tangerine canker.
The active ingredient of the composition is made up of Zhongshengmycin and methyl jasmonate, Zhongshengmycin and methyl jasmonate
Weight ratio is 3-20:1, it is preferred that the weight of Zhongshengmycin and methyl jasmonate ratio is 4:1 and 8:1.
The percentage by weight of described Zhongshengmycin in the composition is 2-10%, the weight of methyl jasmonate in the composition
Amount percentage is 0.5-5%.
Described composition, which adds appropriate adjuvant, can be processed into agriculturally acceptable any formulation, preferably wettable
Property pulvis.
The adjuvant that described composition is prepared into wettable powder needs includes filler, binding agent, dispersant, wetting
Agent, disintegrant or it is other be beneficial to active ingredient in the formulation stablize and drug effect play material, be all in field of pesticide preparation
Commonly use or allow the various compositions used.
The present invention has advantages below and significant technique effect:
(1) active component Zhongshengmycin is different with the mechanism of action of methyl jasmonate, and mutual mixture germ is not likely to produce anti-
Property；Indoor biometricses result shows that Zhongshengmycin and methyl jasmonate rational mixture are obvious to citrus bacterial canker disease germ synergy, favorably
In reducing applications of pesticide amount and reducing pesticide cost, extend the life cycle of Zhongshengmycin.
(2) methyl jasmonate as in plant endogenous growth regulators mass-energy improve plant to pest and disease damage, arid,
Degeneration-resistant reaction under the conditions of high temperature, low temperature etc., is favorably improved via plant immunity, is advantageous to mitigate the development of disease.
Embodiment
Technical scheme and beneficial effect are described further with reference to embodiments, of the present invention hundred
Ratio is divided to be weight percentage.
First, embodiment is prepared
Embodiment 1：5% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 4.0%, methyl jasmonate 1.0%, alkylnaphthalene sulfonate 5.0%, dodecyl are weighed according to quantity
Sodium sulphate 3.5%, white carbon 10%, diatomite complements to 100%.
Embodiment 2：4.5% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 4%, methyl jasmonate 0.5%, alkyl benzene sulfonate calcium salt 2.5%, dodecyl are weighed according to quantity
Sodium sulphate 4.5%, bentonite complements to 100%.
Embodiment 3：12.5% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 10%, methyl jasmonate 2.5%, alkyl benzene sulfonate calcium salt 4.5%, dodecane are weighed according to quantity
Base sodium sulphate 5%, kaolin complements to 100%.
Embodiment 4：10.5% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 10%, methyl jasmonate 0.5%, lignosulfonates 6%, spaonin powder are weighed according to quantity
4%th, white carbon 10%, kaolin complement to 100%.
Embodiment 5：8.5% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 8.0%, methyl jasmonate 0.5%, lignosulfonates 2.5%, dodecyl are weighed according to quantity
Benzene sulfonic acid sodium salt 2.5%, kaolin complements to 100%.
Embodiment 6：15% Zhongshengmycin methyl jasmonate wettable powder
Formula：Zhongshengmycin 10.0%, methyl jasmonate 5.0%, alkylnaphthalene sulfonate 8%, dodecyl are weighed according to quantity
Sodium sulphate 3.5%, white carbon 10%, diatomite complements to 100%.
Above-described embodiment 1-6 preparation method is to have weighed each composition by formula rate, is well mixed in mixed cylinder,
Remixed after airslide disintegrating mill crushes uniformly, you can Zhongshengmycin methyl jasmonate wettable powder product is made.
2nd, BIOLOGICAL ACTIVITY EXAMPLES
The method that the biological activity test of the present invention is combined using indoor biometricses toxicity test and field medicine effect test.
Embodiment 1：The Toxicity Determination of Zhongshengmycin, methyl jasmonate and built agent to citrus processing
Subjects：Citrus processing (Xanthomonas citri (Hahse)) (field is picked up from, and it is pure through separating
Change)
Test method：Experiment reference《Pesticide bioassay technology》(old spring chief editor, publishing house of Beijing Agricultural University go out
Version), and People's Republic of China (PRC) agricultural industry criteria NY/T1156.16-2008《Farm-chemical indoor determination test rule is killed
The part of microbial inoculum the 16th：Bacteria growing inhibiting amount tests turbidity method》, Zhongshengmycin, methyl jasmonate are determined using turbidity method
And mixture is to the indoor virulence of citrus processing.
A, the culture of non-target bacteria：From NA culture mediums (nutrient agar) culture medium, (1LNA culture mediums contain polyprotein
Peptone 5g, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, pH7.0 is adjusted to 10mol/L NaOH) in 30 DEG C of bacteriums
Passivation culture citrus processing 48h, picking single bacterium are fallen within 30ml NA fluid nutrient mediums in incubator, are placed in 28 DEG C -30 DEG C
Under the conditions of concussion and cultivate (120r/min) 18-30h to target bacteria growing to exponential phase.
B, medicament Zhongshengmycin and methyl jasmonate treatment and culture：Zhongshengmycin mother's medicine is directly dissolved as 20 with distilled water
μ g/ml mother liquors, set 5 series mass concentration gradients, and the Tween-80 aqueous solution of methyl jasmonate 0.1% is diluted to 80 μ g/ml
Mother liquor, 5 series mass concentration gradients are set, add medicament in 30ml NA fluid nutrient mediums by the concentration quantitative of design, often
Processing sets 4 repetitions, and sets the processing without active ingredient and make blank control (CK).It is inoculated with respectively into each processing culture medium
The non-target bacteria 100ul bacterium solutions of exponential phase, concussion and cultivate (120r/min) 16-20h is not under the conditions of being placed in 28 DEG C -30 DEG C
When the control bacteria growing of adding medicine reaches exponential phase, determine and record the turbidity of each processing.
The measure of turbidity：Determine chemicals treatment before and processing after bacterium 600nm absorbance value.
Computational methods：
Statistical analysis is carried out with DPS data processing softwares, calculates the EC of each medicament50, prevention effect is converted into niqueMin
(y), liquor strength (ug/ml) is converted into logarithm value (x), concentration EC in being calculated virulence equation with least square method and suppressed50, so
The toxicity index and co-toxicity coefficient (CTC) of medicament are calculated by the abundant methods of Sun Yun afterwards.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
A percentage composition+B medicament toxicity index in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
B percentage composition in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
When CTC≤80, composition shows as antagonism, when 80<CTC<120, composition shows as summation action, when
CTC >=120, composition show as synergistic effect.
Measurement result is shown in Table 1.
Table 1：The Toxicity Determination table of Zhongshengmycin, methyl jasmonate and built agent to citrus bacterial canker disease
Measurement result shows, when the proportioning of Zhongshengmycin and methyl jasmonate is 3-20:When 1, co-toxicity coefficient (CTC) is more than
120, when the weight of Zhongshengmycin and methyl jasmonate ratio is 4:1、8:When 1, co-toxicity coefficient 170.7,175.0, mixed agent pair
The synergy of citrus bacterial canker disease is obvious, and both mixtures advantageously reduce the dosage of each single dose, there is good guidance in field test
Value.
Embodiment 2：Prepare the field test of the preparation preventing and treating granulated sugar tangerine canker of the gained of embodiment 1
Experiment is located at Nanning suburbs, 9-10 month high temperature and rainy canker full incidence periods in 2015.By 5% Zhongshengmycin
Methyl jasmonate wettable powder is watered 1500 times of dilution, and in granulated sugar tangerine canker early stage, even spraying is carried out to plant,
It is sufficiently humidified so as to blade as standard, 7d is sprayed 1 time again after first time medicine, altogether dispenser 2 times, and the preventing and treating fruit of 15 days is after last medicine
89.6%.The Zhongshengmycin wettable powder of comparison medicament 5% is watered 1000 times of dilution, 5% methyl jasmonate wettable powder is converted
Water dilutes 300 times, and using identical application method, the preventive effect of 15 days is respectively 73.5% and 68.2% after last medicine.
Control single dose is significantly better than to the preventive effect of granulated sugar tangerine canker after this explanation Zhongshengmycin and methyl jasmonate compounding,
Zhongshengmycin and methyl jasmonate rational mixture are advantageous to improve drug effect, reduce pesticide cost.
Embodiment 3：Prepare the field test of the preparation preventing and treating horse water tangerine canker of the gained of embodiment 2
Experiment is located at Lipu county of hazard prevention, and 8-19 month high temperature and rainy seasons in 2016 are in canker full incidence period.Will
4.5% Zhongshengmycin methyl jasmonate wettable powder is watered 1500 times of dilution, in horse water tangerine canker early stage, to horse
Water tangerine plant carries out even spraying, is sufficiently humidified so as to blade as standard, and 7d is sprayed 1 time again after the 1st medicine, altogether dispenser 2 times, last
The prevention effect of 15 days is 87.5% after medicine.The Zhongshengmycin wettable powder of comparison medicament 5% is watered dilution 1000 times, 5% jasmine
Jasmine acid methyl esters wettable powder is watered 300 times of dilution, and using identical application method, the prevention effect of 15 days is respectively after last medicine
72.6% and 64.82%.
It is single significantly better than control to the preventive effect of horse water tangerine tangerine canker after this explanation Zhongshengmycin and methyl jasmonate compounding
Agent, Zhongshengmycin and methyl jasmonate rational mixture are advantageous to improve drug effect, reduce pesticide cost, are advantageous to alleviate germ confrontation
The resistance of raw plain class agricultural chemicals Zhongshengmycin, extends its life cycle.
In experiment, to citrus fruit trees safety, growth has no adverse effects test medicine, therefore is worth pushing away in agricultural production
Wide application.
Claims (4)
1. the composition containing methyl jasmonate is in the application of citrus bacterial canker disease.
2. application according to claim 1, it is characterised in that described composition active ingredient is by Zhongshengmycin and jasmine
The weight ratio of sour methyl esters composition, Zhongshengmycin and methyl jasmonate is 3-20:1.
3. application according to claim 2, it is characterised in that the weight of Zhongshengmycin and methyl jasmonate ratio is 4:1 He
8:1。
4. application according to claim 2, it is characterised in that the percentage by weight of Zhongshengmycin in the composition is 2-
10%, the percentage by weight of methyl jasmonate in the composition is 0.5-5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710429983.4A CN107333783A (en) | 2017-06-09 | 2017-06-09 | Application of the composition containing methyl jasmonate in citrus bacterial canker disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710429983.4A CN107333783A (en) | 2017-06-09 | 2017-06-09 | Application of the composition containing methyl jasmonate in citrus bacterial canker disease |
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| CN107333783A true CN107333783A (en) | 2017-11-10 |
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| CN201710429983.4A Pending CN107333783A (en) | 2017-06-09 | 2017-06-09 | Application of the composition containing methyl jasmonate in citrus bacterial canker disease |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041058A (en) * | 2017-12-25 | 2018-05-18 | 广西壮族自治区农业科学院园艺研究所 | A kind of microbicide compositions for preventing citrus bacterial canker disease |
| CN113367146A (en) * | 2021-05-11 | 2021-09-10 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101171931A (en) * | 2007-12-06 | 2008-05-07 | 张少武 | Zhongshengmycin wettable powder and preparation method thereof |
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2017
- 2017-06-09 CN CN201710429983.4A patent/CN107333783A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101171931A (en) * | 2007-12-06 | 2008-05-07 | 张少武 | Zhongshengmycin wettable powder and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 朱宏涛等: "激素类农药茉莉酸及其甲酯的植物生物活性及其在农业生产中的应用", 《农药》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041058A (en) * | 2017-12-25 | 2018-05-18 | 广西壮族自治区农业科学院园艺研究所 | A kind of microbicide compositions for preventing citrus bacterial canker disease |
| CN113367146A (en) * | 2021-05-11 | 2021-09-10 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
| CN113367146B (en) * | 2021-05-11 | 2022-08-05 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
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