CN113207889A

CN113207889A - Bactericidal composition containing ethylicin and tebuconazole and application thereof

Info

Publication number: CN113207889A
Application number: CN202110685672.0A
Authority: CN
Inventors: 孙海燕; 陈怀谷; 邓渊钰; 李伟; 曹淑琳
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2021-06-21
Filing date: 2021-06-21
Publication date: 2021-08-06

Abstract

The invention relates to a sterilization composition containing Ethylicin (Ethylicin) and Tebuconazole (Tebuconazole) as effective active ingredients, wherein the mass ratio of the Ethylicin to the Tebuconazole is (20-1): 1 to 20. The bactericidal composition has a good control effect on wheat scab, and can also control wheat powdery mildew and rust disease, improve the yield of wheat and improve the quality of wheat. The action mechanism of ethylicin in the bactericidal composition is different from that of the existing bactericide, and the bactericidal composition is safe and can delay the generation of the resistance of the existing bactericide.

Description

Bactericidal composition containing ethylicin and tebuconazole and application thereof

Technical Field

The invention belongs to the field of pesticides, and particularly relates to a bactericidal composition containing ethylicin and tebuconazole and application thereof in prevention and treatment of wheat scab.

Background

Ethylicin (ethylicin) is a plant bionic pesticide, and is allicin extract and ethyl homolog of allicin. Ethylicin has high-efficiency sterilization effect, has plant growth regulation effect, and can improve seed germination rate, promote growth, enhance yield, improve quality, etc. At present, the method is mainly registered for preventing and treating leaf spot and brown spot on fruit trees, rice blast and rice rot on rice, and blight and verticillium wilt on cotton. It has not been registered on wheat at present.

Tebuconazole (Tebuconazole) is a bactericidal agent that is an ergosterol synthesis inhibitor. The bactericide has wide prevention and control spectrum, good systemic property and difficult generation of drug resistance. Tebuconazole has been used for preventing and treating wheat diseases in China since the 70 s, and is used particularly in recent years.

Wheat scab is one of the most threatening epidemic diseases in wheat production in China. The prevalence of the disease can not only cause great reduction of yield of wheat, but also cause pollution of grains by various toxins such as Deoxynivalenol (DON) and the like, thereby seriously threatening the health of people and animals. Wheat scab in China mainly occurs in winter wheat areas in the middle and lower reaches of Yangtze river and in spring wheat areas in the northeast of China. In recent years, wheat scab rapidly spreads to Huang-Huai-Mai district due to the influence of climate warming, straw returning and the like.

The planting of resistant varieties is an economic and safe effective method for preventing and treating wheat scab, but at present, no wheat variety with high resistance to the scab exists, and the planted varieties with low and medium resistance cannot completely resist the prevalence and harm of diseases due to the influence of multiple factors such as climate factors, cultivation system changes and the like. Chemical control remains the most effective method for controlling wheat scab at present. The current agents for controlling wheat scab are: cyanoxastrobin-tebuconazole, prothioconazole-tebuconazole, fluxapyroxad. Wherein, the cyhalothrin and the fluxapyroxad belong to medicaments which are easy to generate drug resistance. And the residual period of the agents is long, so that the agents are not suitable for the wheat filling period. The prevention and control of the wheat in the grain filling period are also very important, so that the safe and efficient bactericide is developed, has a different action mechanism from the existing bactericide, and has important significance for delaying the generation of the bactericide resistance and effectively controlling the wheat scab. In this context, as a project group for studying wheat diseases, we conducted the study of the present invention.

The invention content is as follows:

aiming at the prevention and treatment of wheat scab, the compound bactericide which is environment-friendly, good in effect and stable is provided.

The invention is realized by the following steps: the bactericidal composition containing ethylicin and tebuconazole and the application thereof are composed of active ingredients and auxiliary ingredients of pesticide preparations, and particularly, the active ingredients are prepared from the ethylicin and the tebuconazole in a weight ratio of 20-1: 1-20, and the active ingredient accounts for no more than 90% of the weight of the bactericidal composition.

Preferably, the active ingredients comprise ethylicin and tebuconazole in a weight ratio of 10-1: 1-10; more preferably, the active ingredients comprise ethylicin and tebuconazole in a weight ratio of 5-1: 1-5; further preferably, the active ingredients comprise ethylicin and tebuconazole in a weight ratio of 2-1: 1 to 2.

According to the invention, the bactericidal composition can be wettable powder, water dispersible granules, a suspending agent, an aqueous emulsion or missible oil.

The invention has the advantages that:

(1) the ethylicin single agent and the compound agent of the ethylicin single agent and the tebuconazole have obvious prevention and control effects on wheat scab;

(2) the cost of ethylicin is low;

(3) ethylicin is environment-friendly;

(4) the ethylicin is different from the existing bactericide for preventing and treating wheat scab in action mechanism, and can delay the generation of resistance of wheat scab to the existing bactericide by compounding or alternatively using the ethylicin and the existing bactericide.

Detailed Description

Example 1 ethylicin has a certain inhibitory activity against the growth of hyphae of wheat scab.

Mixing ethylicin and PDA culture medium uniformly, and preparing into the mass concentration of 0, 5,10,20,40,60,80 μ g/mL^-1The plate of (2). Preparing a bacterial dish with the diameter of 5mm from the pre-cultured wheat scab germs near the edge, inoculating the bacterial dish in the center of a flat plate, and culturing at the constant temperature of 25 ℃ for 3 days. Each treatment was repeated 3 times. After 3 days, the colony diameter of each treatment was measured by the cross method, and the inhibition rate was calculated.

As shown in Table 1, ethylicin has a certain inhibitory activity on the mycelial growth of Gibberella tritici, EC₅₀About 20μg·mL^-1。

TABLE 1 mycelial growth inhibitory Activity of ethylicin against Gibberella tritici

Ethylicin concentration (μ g/mL)^-1)	Diameter of colony growing for 3 days (cm)	Inhibition ratio (%)
			CK	6.25
5	4.75	26.09
			10	3.85	41.74
20	3.45	48.70
			40	2.80	60.00
80	2.00	73.91

Example 2 ethylicin has a good inhibitory activity on spore germination of wheat scab.

Mixing ethylicin with water agar medium, and making into mixture with mass concentration of 0, 1,2,3,4,6,8, 8 μ g/mL^-1The plate of (2). Pre-cultured wheat scab germ conidia are coated on a drug-containing flat plate, and after the wheat scab germ conidia are cultured for 10 hours at a constant temperature of 25 ℃, the inhibition condition of ethylicin with each concentration on spore germination is observed under a microscope.

The results showed 3. mu.g.mL^-1The ethylicin completely inhibits the germination of the conidium of the wheat scab germ.

Example field control of 380% Etallicin emulsifiable concentrate against wheat scab

Wheat variety to be tested: and 5, wheat winnowing 25. The experimental place is Zhenjiang sentence capacity and Nanjing Liuhe. The experiment was set up in 3 replicates, each cell area being 20m². Spraying water with water amount of 450kg/hm at the initial flowering stage and full flowering stage of wheat respectively². The investigation was started 25 days after the application. The wheat scab investigation method comprises the following steps: sampling is carried out at random at 5 points in each cell, 100 ears are surveyed at each point, and 500 ears are surveyed in each cell. The following grading criteria were investigated: level 0: no disease; level 1: the area of the diseased ear accounts for less than 25% of the total area of the ear; and 3, level: the area of the diseased ear accounts for 25-50% of the total area of the ear; and 5, stage: the area of the diseased ear accounts for 50% -75% of the total area of the ear; and 7, stage: the area of the diseased ear accounts for more than 75% of the total area of the ear.

Disease index is 100 × Σ (number of diseased ears (leaves) × corresponding number of stages)/(number of total examined ears (leaves) × total number of stages);

disease means control effect (%) (control disease index-treatment disease index)/control disease index × 100.

The obtained data is subjected to difference significance analysis by a Duncan's new double-pole difference method of a DPS data processing system.

The results show that: the 80% ethylicin emulsifiable solution has a certain field control effect on wheat scab, and can be used for controlling wheat powdery mildew and wheat rust.

Example 4 field test of Complex formulation of ethylicin and Tebuconazole for controlling wheat scab

The test provides a 30% ethylicin tebuconazole suspending agent, wherein the ethylicin is 20% and the tebuconazole is 10%.

The test is carried out at the fragrance test base of Zhenjiang agricultural academy of sciences. Wheat variety to be tested: and 5, wheat winnowing 25. The experiment was set up in 3 replicates, each cell area being 20m². Spraying water with water amount of 450kg/hm at the initial flowering stage and full flowering stage of wheat respectively². The investigation was started 25 days after the application. The investigation method is as above.

The results show that: the 30% ethylicin tebuconazole suspending agent has better prevention and treatment effect on wheat scab.

Example 5 field test of Complex formulation of ethylicin and Tebuconazole for controlling wheat scab

The test provides a 30% ethylicin tebuconazole emulsifiable concentrate, wherein the ethylicin is 20% and the tebuconazole is 10%.

The test is carried out in the experimental base of agricultural academy of sciences of Jiangsu province in Liuhe region of Nanjing city. Wheat variety to be tested: and 5, wheat winnowing 25. The experiment was set up in 3 replicates, each cell area being 20m². The investigation method is as above.

The results show that: the 30% ethylicin tebuconazole emulsifiable solution has better control effect on wheat scab.

The ethylicin and tebuconazole compound agent has the effect of preventing wheat powdery mildew and rust disease.

TABLE 2 preventing and treating effect of ethylicin and tebuconazole compound agent on wheat scab 2021

The test results in the above examples show that ethylicin has good in vitro inhibition effect and field control effect on wheat scab, and the compound preparation of ethylicin and tebuconazole has good and stable control effect on wheat scab, and can also control wheat powdery mildew and wheat rust.

Claims

1. A bactericidal composition containing ethylicin and tebuconazole, which consists of active ingredients and auxiliary ingredients of pesticide preparations, is characterized in that: the active ingredients comprise ethylicin and tebuconazole according to the weight ratio: 20-1: 1-20, and the active ingredient accounts for no more than 90% of the weight of the bactericidal composition.

2. The germicidal composition containing ethylicin and tebuconazole according to claim 1, characterized in that: the active ingredients are ethylicin and tebuconazole in a weight ratio of 10-1: 1 to 10.

3. The germicidal composition containing ethylicin and tebuconazole according to claim 2, characterized in that: the active ingredients are ethylicin and tebuconazole in a weight ratio of 5-1: 1 to 5.

4. The germicidal composition containing ethylicin and tebuconazole according to claim 3, characterized in that: the active ingredients are ethylicin and tebuconazole in a weight ratio of 2-1: 1 to 2.

5. The germicidal composition containing ethylicin and tebuconazole according to any one of claims 1 to 4, characterized in that: adding bactericide auxiliary agent commonly used in agriculture to prepare bactericide formulation acceptable in agriculture.

6. The germicidal composition containing ethylicin and tebuconazole according to claim 5, characterized in that: the composition can be wettable powder, water dispersible granules, suspending agents, aqueous emulsion or missible oil.

7. The germicidal composition containing ethylicin and tebuconazole according to claim 6, characterized in that: the active component accounts for 15-80% of the weight of the sterilization composition.

8. The use of the germicidal composition containing ethylicin and tebuconazole according to claim 7, characterized in that: can be used as a control agent of wheat scab.

9. The use of the germicidal composition containing ethylicin and tebuconazole according to claim 8, characterized in that: can prevent and treat both wheat rust and wheat powdery mildew.