CN112673917A - Method for cultivating virus-free first-generation fruit seedlings by greenhouse cultivation of lily tissue-cultured rooted seedlings - Google Patents
Method for cultivating virus-free first-generation fruit seedlings by greenhouse cultivation of lily tissue-cultured rooted seedlings Download PDFInfo
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Abstract
The invention discloses a method for cultivating a first-generation virus-free bulb fruit seedling in a greenhouse for lily tissue culture rooted seedlings, which comprises the steps of site selection and building of a seedling greenhouse, disinsection, disinfection and sterilization of soil in the greenhouse and adjustment of the pH value of the soil, suspension tillage, ridging, ditching and fine sand paving of the soil in the greenhouse, planting and early-stage management of the rooted seedlings, management before budding, management after budding, fruit picking and virus-free first-generation fruit seedling and storage. The invention is one of the indispensable key links of lily quick tissue culture propagation seedling culture and excellent seedling large-scale industrialization, and is the first step of lily tissue culture rooted seedlings from a tissue culture laboratory to field planting. The invention can provide a large quantity of strong, duplicate-reducible, high-yield, high-quality and virus-free first-generation lily bulb seedlings with little plant diseases and insect pests and no bacterial virus infection for growers, and solves the problems of traditional field bulb separation, scale embedding and the like in traditional lily planting.
Description
Technical Field
The invention relates to a method for culturing crop seedlings in a large scale, in particular to a seedling culturing method for culturing first-generation detoxified lily bulbs in a greenhouse for lily tissue culture rooted seedlings.
Background
Lily is a perennial herb of Liliaceae, and is suitable for growing in sandy loam with deep soil layer, good drainage and loose and fertile soil. The subterranean bulb of lily is rich in starch, protein, fat and various vitamins and minerals required by the human body. According to records of medical records of Jinkui Yao, Ben Cao gang mu and Chinese pharmacopoeia, lily has the effects of moistening lung, relieving cough, calming heart, tranquilizing mind, regulating spleen, invigorating stomach, strengthening middle-jiao, replenishing qi, facilitating urination and defecation, relieving innominate toxic swelling, stopping bleeding and the like. Modern medical research proves that colchicine, colchicine and the like contained in lily have obvious inhibiting effect on mitosis of human cells, and have obvious curative effect on inhibiting cancer cells, acute gout and the like. Bulbus Lilii can also be used for extracting glucose and quinine.
China is the origin of lily, and about half of the lily varieties come from China globally. The national lily is planted in the long-term folk, and the method mainly comprises conventional breeding and planting methods such as bulb separation, scale embedding and the like in the field; after the lily is planted for multiple generations, the variety degeneration is serious, the yield of the bulb fruits is obviously reduced, and the bacterial virus infection and plant diseases and insect pests are increased.
According to the traditional bulb division, scale embedding and other seed reserving planting methods, accumulated bacterial virus infection of soil and bulb fruits and plant diseases and insect pests are increasingly serious, the disease resistance of plants is weak, a large amount of pesticides need to be sprayed in the planting process, and the soil and the lily bulb fruits have more pesticide residues, so that the human health is influenced.
On the basis of completing variety optimization, a modern method of tissue rapid propagation is adopted, so that the problems can be fundamentally solved. The whole process of rapid propagation and breeding of lily bulbs comprises the following steps: the method comprises the steps of lily variety optimization and promotion → bulb cone cleaning, peeling → scale cleaning, disinfection and detoxification → yellow matrix explant induction bulblet → bulblet proliferation → differentiated seedling strengthening and rooting → seedling hardening → rooted seedling cultivation in a greenhouse for first-generation detoxified fruit seedlings (or second-generation detoxified bulb seedlings cultivation in the open air) → open air cultivation. The process shows that the greenhouse cultivation of the lily bulb first-generation fruit seedlings of the lily tissue culture rooted seedlings is an indispensable key link of the whole industrial chain of rapid tissue culture propagation seedling cultivation and lily planting, and is the first step of field planting of the lily tissue culture seedlings from a tissue culture laboratory. Because the rooted seedlings need to be cultivated in a greenhouse, the survival rate is high. The existing field planting adopts the methods of bulb splitting, scale embedding and the like of lily bulb for direct planting, and does not adopt the planting method of tissue culture seedling raising, so that the problems of more bacteria and diseases and insect pests and low yield exist, and a large quantity of strong, duplicate reduction property, high yield, few diseases and insect pests and high-quality virus-free first-generation lily bulb seedlings without bacterial and virus infection cannot be provided for growers. In order to solve the problems caused by traditional breeding and planting of lily and achieve successful cultivation of lily rooted seedlings in a greenhouse, the applicant explores a set of successful experience, method and seedling planting technical specifications. The scheme is generated.
Disclosure of Invention
The invention aims to provide a method for cultivating virus-free first-generation fruit seedlings in a greenhouse of lily tissue culture rooted seedlings, which can solve the problems caused by traditional breeding and planting of lily and achieve successful cultivation of lily rooted seedlings in the greenhouse.
In order to achieve the above purpose, the solution of the invention is as follows:
a method for cultivating virus-free first-generation fruit seedlings in a greenhouse by tissue culture rooted seedlings of lily comprises the following steps:
first, site selection and construction of seedling raising greenhouse
Selecting a land plot with the altitude of 200 plus 400 meters to build a seedling raising greenhouse;
secondly, killing insects, sterilizing and adjusting the pH value of soil in the shed;
thirdly, hanging and ploughing the crushed soil, forming ridges and ditching, and paving fresh water fine sand or fine soil on the surfaces of the ridges;
fourth, planting and early-stage management of rooted seedlings
Taking out the acclimatized rooted seedlings from the bottle, washing the culture medium with tap water, and placing the culture medium in a container with the volume ratio of 1: soaking in carbendazim solution of 1500 for 5 min, taking out, and filtering to dry;
planting the drained rooting seedlings on ridges of a seedling raising greenhouse, wherein the planting depth is 2-3cm, the planting density per square meter is about 160-180 rooting seedlings, and the row spacing and the plant spacing are about 7.5-8 cm;
irrigating the rooted seedlings with rooting water on the same day as the planting of the rooted seedlings; on the 1 st to 10 th days, the illumination condition is controlled to be 4000-; after 10-15 days, the greenhouse naturally transmits light in the morning and evening, the illumination intensity is controlled to be 6000-; after 15-20 days, receiving natural light;
management before sprouting
After a certain period of planting, the air is cooled, after the air temperature is reduced to a certain degree, the old leaves of the rooted seedlings shrink and fall off, before new bud leaves grow, a sunshade net can be folded, the greenhouse is subjected to normal irradiation of sunlight, the temperature in the greenhouse cannot exceed 32 ℃ in the daytime, the greenhouse is required to be ventilated, the soil is kept moist, heat preservation is carried out intentionally when the greenhouse is cooled, artificial weeding is carried out for 1-2 times in the period, and the conventional management of the greenhouse is usually carried out;
sixthly, management after sprouting
In the next 2-3 months, applying a first thin fertilizer within 5-10 days after the underground bulblets grow new buds, wherein the first thin fertilizer mainly comprises a high-nitrogen low-phosphorus low-potassium compound fertilizer, and applying a second fertilizer in about 5 months, wherein the low-nitrogen high-phosphorus high-potassium compound fertilizer is mainly used; the soil is required to be kept moist, the greenhouse is ventilated to prevent diseases and insect pests, and the greenhouse is subjected to conventional management;
seventhly, starting fruits, harvesting detoxified first-generation fruit seedlings and keeping
In 8-9 months of the next year, first-generation fruit seedlings of lily bulb corms are obtained, and each plant can obtain 30-60 g of first-generation fruit seedlings of the detoxified lily bulb; the obtained detoxicated first-generation fruit seedlings are put in a plastic turnover frame and stored in a cool and ventilated place or a cold storage, and the seedlings are prevented from being smoldered.
Furthermore, in the first step, the seedling raising greenhouse is constructed by selecting sandy loam plots which are convenient to drain, have fall, are not flooded, have more soil organic matters and humus, have granular structures, are loose and have good air permeability, have clean water sources nearby, are convenient to traffic, are easy to level terrain, have sufficient sunlight and have a pH value close to neutral.
Further, in the first step, each standard seedling raising greenhouse is 8M wide, 50M long and 400 square in area, the minimum net height in the greenhouse is not lower than 2.8M, a sun shading system is arranged outside the greenhouse, the top and the side surfaces of a transparent film are covered, insect-proof nets are arranged on the side surfaces, and an automatic atomization spraying system is arranged in the greenhouse.
Further, in the second step, the tea seed cake dregs are used for soaking water to kill insects on the soil in the greenhouse, lime powder is broadcast to sterilize the greenhouse and adjust the pH value of the soil, so that the soil is neutral and slightly alkaline.
Further, in the third step, the land in the shed is subjected to suspension ploughing, soil crushing and ridging, the ploughing depth is about 20cm, the ridge width is 1.2-1.3 m, the furrow depth is 20cm, and the furrow width is 30 cm.
Further, in the third step, after ridging and ditching are well leveled, a layer of fresh water fine river sand or fine soil with the thickness of 1-2cm is paved on the upper surface of the ridge, and the paved ridge surface is required to be leveled.
Further, in the sixth step, the first fertilizing amount is 10-17 jin per mu, and the second fertilizing amount is 17-25 jin per mu.
Further, in the fourth step, 8, 9, 10 and 11 months per year are the best seasons for planting the rooted seedlings, and proper planting time is selected according to the climate of each place.
The invention is one of the indispensable key links of lily quick tissue culture propagation seedling culture and excellent seedling large-scale industrialization, and is the first step of lily tissue culture rooted seedlings from a tissue culture laboratory to field planting. By adopting the method, the invention can provide large-batch strong first-generation fruit seedlings of high-quality virus-free lily bulbs with copy reduction property, high yield, less plant diseases and insect pests and no bacterial virus infection for farmers, and solves the problems of bulb separation, scale embedding and the like of the traditional field lily planting.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following specific examples.
First, site selection and construction of seedling raising greenhouse
The plot with the altitude of 200-400 meters is selected to construct the greenhouse to be most suitable, the lily is planted in the land with the altitude, the lily diseases and insect pests are few, the lower the altitude is, the more the plant diseases and insect pests are, the use amount of pesticides can be increased, and the planting cost can be increased.
The land is selected to be drained conveniently, and the land with fall, no dark surge in the land, no flooding and sandy loam is selected; the soil requires more organic matters and humus, and has loose granular structure, good air permeability and nearly neutral pH value; the large-area greenhouse building needs to build a seedling raising greenhouse on the land which can be in large area, has clean water sources (reservoir water, underground water and the like) nearby, is convenient to transport, is easy to level and has sufficient sunlight. In the embodiment, a seedling raising greenhouse is built in Yanming lake town of Tuhua city in Yanbian, and the altitude is about 380 meters.
Each standard seedling raising greenhouse is 8M in width, 50M in length, 400 square in area and single-arch arc-shaped, and the transverse distance between the greenhouses is about 3.2 meters; in order to meet the requirements of mechanized operation of furrowing in suspension-ploughing soil-breaking ridge lands and lily bulb fruit-picking in a greenhouse, the minimum clear height in the greenhouse is not lower than 2.8M and generally between 2.8 and 3.2M, an automatic sunshade net system, a transparent film cover top and side surfaces are required to be arranged outside the greenhouse, the side films can be retracted, an insect-proof net is arranged on the inner side, furrowing is required to be arranged on the long transverse edge outside the greenhouse, the road land between the greenhouses is leveled, a clean water source and a reservoir are required to be arranged nearby, an automatic atomization spraying system is arranged at a height of about 3.2 meters in the greenhouse, and the greenhouse is provided with power supplies and.
Secondly, disinsection, disinfection and sterilization of the soil in the shed and adjustment of the pH value of the soil
Killing pests on the land in the shed. According to the invention, tea seed cake residue soaking water is used for ecologically and nonhazardous disinsection of the soil in the greenhouse, so that the greenhouse is more ecological and environment-friendly. Before the suspension tillage and soil breaking, the tea seed cake residue soaking water and the soaked residue are sprinkled and spread on the ground in the shed, and the tea seed residue can be infiltrated and buried in the soil during the suspension tillage of the land; or the automatic spraying system can be used for spraying the filtered tea seed cake residue leaching water on the soil in the shed, and the leached residue is then scattered on the ground. Using the squeezed tea seed bulk material, and soaking for one day; the tea seed cake is soaked for 2-3 days. The using amount of each 8M standard greenhouse tea seed cake is 50-100 jin, and the using amount can be increased or decreased according to the insect pest condition of the land. In this example, bulk tea seed residue is selected, and the amount of tea seed residue is 55 jin/standard shed. Manually spraying the tea seed residue leaching water on the land in the shed, and then scattering the leached residue on the ground.
Sterilizing the soil in the shed and adjusting the pH value. Before suspension tillage and soil crushing, spreading lime powder on the land in the shed for disinfection and sterilization; the lime consumption of each 8M by 50M standard greenhouse is 30-60 jin, the lime consumption is increased or decreased according to the soil chemical components and the pH value of the soil, and the neutral weak alkalinity of the soil after lime spreading is better. The amount of lime powder used in this example is 35 jin per standard shed.
The soil outside the greenhouse can be used for killing insects by using an insecticide or tea seed cake dregs, and lime powder is sown for disinfection and sterilization. In this example, tea seed residue is used for killing insects in the land outside the greenhouse, and then is sterilized by lime powder.
Thirdly, hanging ploughing, breaking soil, ridging, ditching and paving fresh water fine sand on the surface of the ridge
After the insect killing, disinfection and sterilization, carrying out suspension tillage, soil crushing and ridging on the soil in the greenhouse by using a suspension cultivator, and ditching by using a ditcher; the cultivation depth is required to be about 20cm, the ridge height is 20cm, the furrow depth is 20cm, the furrow width is 30cm, the ridge width is 1.2-1.3 m, and the ridge length is 12-13 m. The depth of the long transverse edge groove outside the shed is 30cm, and the width of the groove is 30 cm. When the suspension tillage is used for crushing soil, the soil layer is dug to be fine and crushed, the ridges and the ditches are tidy, and the ridge surface is flat.
After ridging and ditching are well leveled, a layer of fine sand or fine soil of the fresh water river with the thickness of 1-2cm is paved on the upper surface of the ridge, and the paved ridge surface is required to be level. In areas with high fresh water sand price, fine sand can not be paved on the upper surface layer of the ridge, but the broken soil on the ridge is required to be finely dug, and plate-shaped broken soil cannot be generated.
In the implementation, the ridge width is 1.3 meters, the ridge length is 12.4 meters, and the ridge is formed in parallel with the long edge (50 meters) of the greenhouse in the same direction; and (3) paving fresh water fine sand with the thickness of 1-2cm on the surface of the ridge.
Fourth, planting and early-stage management of rooted seedlings
After the third step, taking out the refined lily rooted seedlings from the bottle, washing the culture medium with tap water, and performing the third step of: 1500, taking out, filtering or airing after soaking for 5 minutes, wherein the disinfected rooting seedlings cannot be directly sunned under the sunlight.
And (3) planting the drained (dried) rooting seedlings in the prepared seedling raising greenhouse, wherein the seed depth is 2-3cm, the planting density per square meter is about 160-180 rooting seedlings, and the row spacing and the plant spacing are between 7.5cm and 8 cm. When digging seedling pits, the seedling planting pits can be dug in rows by using special small tools, then the rooting seedlings are placed at the bottom of the pits, and fine sandy soil is filled in the pits. When planting, the ridge soil and sand must cover the small bulb, the leaves and seedling core are exposed out of the sand surface or soil surface, and after planting, the seeds are lightly compacted by fingers.
Irrigating the rooted seedlings with rooting water on the same day as the planting of the rooted seedlings; on the 1 st to 10 th days, the illumination condition is controlled to be 4000-; after 10-15 days, the greenhouse naturally transmits light in the morning and evening, the illumination intensity is controlled to be 6000-; after 15-20 days, the lamp was irradiated with natural light.
In this example, 52500 rooted seedlings were planted in an 8M by 50M standard greenhouse with row spacing and plant spacing of 7.5-8 cm. The planting time is 3 days to 6 days in 9 months, and the place is Yanbian Minggui lake in Yanbian Town.
Just before planting, ridge soil is required to be kept wet, but no water is soaked. When drying, the spray can be carried out by an automatic atomization spraying system. And performing routine management in daily life.
In the northeast of Jilin Yanbian, 8 and 9 months per year are the best time for planting the rooting seedlings. In areas of Hunan Long Hui and Hubei Dang Yang, 11 months of each year are the optimal time for planting the rooting seedlings.
Management before sprouting
After the rooting seedlings are planted in a certain period, the temperature gradually drops along with the approach of the solar terms to winter, the weather is cooled, after the temperature drops to a certain degree, old leaves of the tissue culture rooting seedlings slowly shrink and fall off, and bulblet continuously grows and regenerates new roots 2-3cm below the soil. The withering time of the original old leaves of the rooted seedlings is different due to different temperature climates of different regions.
Collecting the sunshade net during the period from old leaves to sprout leaves after withering, and allowing sunlight to irradiate naturally, wherein the temperature of the greenhouse is not higher than 32 ℃ during the day; if the sun is exposed in the sun in the middle of the day, the sunshade net is released for sunshade and ventilation. In cold weather, the films on the two sides of the greenhouse are put down for heat preservation and moisture preservation, and certain temperature in the greenhouse is kept; during this time, soil continued to be kept moist.
For example, the weed grows on the ridge, the artificial weed pulling is carried out for 1-2 times. And performing routine management of the greenhouse daily.
In this embodiment, the sun-shading net is closed to allow sunlight to naturally irradiate 30 days before 20 th day and 10 months after the rooted seedlings are planted. After 10 months and 20 days, the greenhouse film is sealed and keeps warm, and the soil in the greenhouse is kept moist and is moderately ventilated occasionally in the noon. During the period, the grass is pulled out once.
Sixthly, daily management after budding
After the foliage of the rooted shoots wither, new shoots and leaves will re-grow from the underground bulblets, typically until the next 2-3 months of the year. When the new buds grow for 5-10 days, applying a first thin fertilizer, wherein the first fertilizer is preferably a high-nitrogen low-phosphorus low-potassium compound fertilizer; the fertilizing amount of each standard greenhouse with 8M by 50M is about 6-10 jin, namely 10-17 jin per mu. The special fertilizer for lily is preferably selected.
Applying a second fertilizer according to the growth condition in 5 months, wherein the second fertilizer is mainly a low-nitrogen high-phosphorus high-potassium compound fertilizer; the fertilizing amount of each standard greenhouse with 8M by 50M is 10-15 jin, namely 17-25 jin per mu. The special fertilizer for lily is preferably selected.
During this period, the soil is kept moist to prevent pests and diseases. And performing routine management of the greenhouse daily.
From seedling feeding to the next 5 months of the year, the lily bulb grows into a bulblet with the weight of about 20 g, in 6-8 months, the bulblet grows rapidly and greatly and grows and weighs, and in 8 months, the bulblet with the weight of about 30-60 g grows out of the ground of the rooted seedling of the lily.
In this example, a high nitrogen, low phosphorus, low potassium compound fertilizer is applied once in the last ten days of 3 months of the following year, using 6 jin/standard shed. In the middle ten days of the month 5, a low-nitrogen high-phosphorus high-potassium compound fertilizer is applied once, and the dosage is 10 jin/standard shed.
Seven, harvesting, collecting first generation fruit seedlings and keeping
In 8-9 months of the next year, the tissue culture rooted seedlings of the lily grow into first-generation fruit seedlings of the virus-free lily bulbs with the weight of about 30-60 g underground after being cultivated in a greenhouse for 10-11 months. The acclimatized rooted seedlings are cultivated and planted in a seedling raising greenhouse, and the survival rate is over 95 percent.
And (4) harvesting the first generation fruit seedlings of the lily bulb corm by using a lily harvesting machine in 8-9 months of the next year, wherein each plant can harvest 30-60 g of the first generation fruit seedlings of the virus-free lily bulb. The harvesting net of the fruit picker needs a screen with small meshes, so that the small lily fruits are not leaked.
The obtained first generation fruits are put into a plastic turnover frame, weighed, labeled and stored in a cool and ventilated place or a refrigeration house for storage. During storage, the seedlings are prevented from being smoldered.
The obtained first-generation virus-free bulb fruit seedlings can be directly supplied to planting households for planting lily commodity fruits in the field; or the second generation fruit seedlings of the virus-free lily bulb corms are planted and cultivated in the open air in a seedling raising base.
In the example, the first generation of fruit seedlings of the detoxified lily bulbs is obtained in the middle ten days of 8 months, each plant grows a bulblet cone, the weight is 30-60 g, the appearance is neat and graceful, and no jute spots exist. The survival rate of the rooted seedlings is about 96 percent.
The invention can provide a large amount of virus-free copy reduction seeds, high yield, less plant diseases and insect pests and high-quality first-generation lily bulb seedlings without bacterial virus infection for growers, and solves the problems of traditional field bulb separation, scale embedding and the like in lily planting.
The above embodiments are not intended to limit the form and style of the present invention, and any suitable changes or modifications made by those skilled in the art should be considered as not departing from the scope of the present invention.
Claims (8)
1. A method for cultivating virus-free first-generation fruit seedlings in a greenhouse by tissue culture rooted seedlings of lily is characterized by comprising the following steps:
first, site selection and construction of seedling raising greenhouse
Selecting a land plot with the altitude of 200 plus 400 meters to build a seedling raising greenhouse;
secondly, killing insects, sterilizing and adjusting the pH value of soil in the shed;
thirdly, hanging and ploughing the crushed soil, forming ridges and ditching, and paving fresh water fine sand or fine soil on the surfaces of the ridges;
fourth, planting and early-stage management of rooted seedlings
Taking out the acclimatized rooted seedlings from the bottle, washing the culture medium with tap water, and placing the culture medium in a container with the volume ratio of 1: soaking in carbendazim solution of 1500 for 5 min, taking out, and filtering to dry;
planting the drained rooting seedlings on ridges of a seedling raising greenhouse, wherein the planting depth is 2-3cm, the planting density per square meter is about 160-180 rooting seedlings, and the row spacing and the plant spacing are about 7.5-8 cm;
irrigating the rooted seedlings with rooting water on the same day as the planting of the rooted seedlings; on the 1 st to 10 th days, the illumination condition is controlled to be 4000-; after 10-15 days, the greenhouse naturally transmits light in the morning and evening, the illumination intensity is controlled to be 6000-; after 15-20 days, receiving natural light;
management before sprouting
After a certain period of planting, the air is cooled, after the air temperature is reduced to a certain degree, the old leaves of the rooted seedlings shrink and fall off, before new bud leaves grow, a sunshade net can be folded, the greenhouse can receive normal irradiation of sunlight, the temperature in the greenhouse can not exceed 32 ℃ in the daytime, the greenhouse is required to be ventilated, the soil is kept moist, heat preservation is carried out when meeting cold, artificial weeding is carried out for 1-2 times, and the conventional management of the greenhouse is usually carried out;
sixthly, management after sprouting
In the next 2-3 months, applying a first thin fertilizer within 5-10 days after the underground bulblets grow new buds, wherein the first thin fertilizer mainly comprises a high-nitrogen low-phosphorus low-potassium compound fertilizer, and applying a second fertilizer in about 5 months, wherein the low-nitrogen high-phosphorus high-potassium compound fertilizer is mainly used; the soil is required to be kept moist, the greenhouse is ventilated to prevent diseases and insect pests, and the greenhouse is subjected to conventional management;
seventhly, starting fruits, harvesting detoxified first-generation fruit seedlings and keeping
In 8-9 months of the next year, first-generation fruit seedlings of lily bulb corms are obtained, and each plant can obtain 30-60 g of first-generation fruit seedlings of the detoxified lily bulb; the obtained detoxicated first-generation fruit seedlings are put in a plastic turnover frame and stored in a cool and ventilated place or a cold storage, and the seedlings are prevented from being smoldered.
2. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: in the first step, the seedling raising greenhouse is constructed by selecting sandy loam plots which are convenient to drain, have fall, are not flooded, have more soil organic matters and humus, have loose granular structures and good air permeability, have clean water sources nearby, are convenient to transport, are easy to level and smooth in terrain, have sufficient sunlight and have a pH value close to neutral.
3. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: in the first step, each standard seedling raising greenhouse is 8M wide, 50M long and 400 square in area, the minimum clear height in the greenhouse is not lower than 2.8M, a sun shading system is arranged outside the greenhouse, a transparent film covers the top and the side faces, an insect-proof net is arranged on the side face, and an automatic atomization spraying system is arranged in the greenhouse.
4. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: and secondly, using tea seed cake dregs to soak water to kill insects on the soil in the greenhouse, and scattering lime powder to sterilize the greenhouse and adjust the pH value of the soil so that the soil is neutral and weakly alkaline.
5. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: and in the third step, the land in the shed is subjected to suspension tillage, soil crushing and ridging, wherein the tillage depth is about 20cm, the ridge width is 1.2-1.3 m, the furrow depth is 20cm, and the furrow width is 30 cm.
6. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1 or 5, which is characterized in that: and in the third step, after ridging and ditching are well leveled, a layer of fresh water fine river sand or fine soil with the thickness of 1-2cm is paved on the upper surface of the ridge, and the paved ridge surface is required to be leveled.
7. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: in the sixth step, the first fertilizing amount is 10-17 jin per mu, and the second fertilizing amount is 17-25 jin per mu.
8. The method for greenhouse cultivation of virus-free first-generation fruit seedlings of lily tissue culture rooted seedlings according to claim 1, which is characterized in that: in the fourth step, 8, 9, 10 and 11 months are the best seasons for planting the rooted seedlings every year, and proper planting time is selected according to the climate of each place.
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