CN112618664A - 一种酶提取式黄连解毒散制备工艺 - Google Patents
一种酶提取式黄连解毒散制备工艺 Download PDFInfo
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Abstract
本发明公开了一种酶提取式黄连解毒散制备工艺,包括以下步骤:将黄连、黄芩、黄柏、栀子、大黄、绞股蓝皂苷、甘草粉碎后;再加入复合酶与适量无水乙醇;将复合酶与中药物混合,加热条件酶解后,匀速搅拌并加入中草药添加剂、活性多肽和甜味剂,待溶剂挥发后,进行固化;于真空干燥器中干燥后,粉碎、过筛、分装即得黄连解毒散;黄连解毒散由以下成分组成:黄连、黄芩、黄柏、栀子、大黄、甘草、中草药添加剂、甜味剂,本发明的黄连解毒散具有泻火解毒和提升免疫力功能,且没有苦味,诱食效果好,主治三焦湿热、疮黄肿毒,广泛应用于马、牛、羊、猪及家禽。
Description
技术领域
本发明属于化学物品制备领域,尤其是涉及一种酶提取式黄连解毒散制备工艺。
背景技术
黄连解毒散是国药精粹,配方经典,在王焘《外台秘要》和孙思邈的《银海精微》等中药名著中均有记载,具有清热解毒、扶正祛邪、袪风除湿,清热凉血,消肿镇痛,提高机体免疫力,增强抗病力,促进畜禽迅速康复之功效,临床对多种畜禽疾病均有很好的防治效果。
黄连解毒散为《中华人民共和国兽药典》2010版二部中收录方剂,由黄连、黄芩、黄柏、栀子四味中药组方而成,具有泻火解毒之功效,主治三焦实热,疮黄肿毒。方中黄连泻上焦心火兼泻中焦火,为君药;黄芩泻上焦肺火,为臣药;黄柏泻下焦肾火,为佐药;栀子通泻三焦之火,导热下行从膀胱而出,为使药。
黄连解毒散主要由黄连、黄芩、黄柏和栀子四味中药组成,具有泻火解毒功能,主治三焦湿热、疮黄肿毒,广泛应用于马、牛、羊、猪及家禽。该散剂对多种细菌均有抑制作用,可用于肺炎、流行性脑脊髓炎、脓毒血症的治疗,对超广谱β-内酰胺酶及持续高产AmpC酶菌株均有不同程度的抑制作用。其中对金黄色葡萄球菌、表皮葡萄球菌、变形杆菌、痢疾杆菌的抑制作用较强。黄连解毒散的抗菌作用机制可能与抑制细菌的DNA合成有关。
现有技术中,如中国发明授权专利文献,授权公告号CN102058708B,该发明属于畜禽中药技术领域,具体涉及一种黄连解毒散固体分散体,其成分为:黄连、黄芩、黄柏、栀子、聚乙二醇6000、泊洛沙姆、甘油或丙二醇,该发明采用超微粉碎技术和固体分散技术制备黄连解毒散固体分散体,并选择合适的载体,有利于黄连解毒散固体分散体有效成分的浸出,增加了药物的分散度和比表面积,从而增加黄连解毒散固体分散体的溶解度、溶出速度,提高了药物生物利用度,有利于调剂、服用和疗效发挥,该发明具有清热解毒、泻火凉血、败毒祛邪、降温止泻、抗菌消炎等功效,可用来治疗由病毒和细菌引起的畜禽三焦实热、疮黄肿毒等热性疾病,但是该解毒散可能存在苦味,不利于禽类摄食,且在提升禽类免疫力方面还具有一定的提升空间。
发明内容
本发明的目的是针对上述技术问题提供一种对细菌有效优异抑制效果,清热解毒、提升免疫力、降温止泻、抗菌消炎、没有苦味,诱食效果好的酶提取式黄连解毒散制备工艺。
为实现上述目的,本发明提供如下技术方案:一种黄连解毒散的制备工艺,包括以下步骤:
1)将黄连、黄芩、黄柏、栀子、绞股蓝皂苷、大黄、甘草清洗,晒干,粉碎备用;
2)将纤维素酶和果胶蛋白酶按质量比1:1于醋酸-醋酸钠缓冲液中;
3)加入三倍量的50%乙醇浸泡药材,并加入复合酶溶液,酶解后,减压抽滤得到滤液后,加入中草药添加剂、活性多肽和甜味剂,待溶剂挥发后,进行固化;
4)于真空干燥器中干燥后,粉碎、过筛、分装即得黄连解毒散;
该黄连解毒散由以下成分及重量份组成:黄连15-25份、黄芩10-30份、黄柏10-30份、绞股蓝皂苷0.1-0.5份、栀子5-15份、大黄10-20份、甘草10-20份、中草药添加剂1-3份、活性多肽0.2-0.4份、甜味剂0.5-1份、混合酶5-10份。
通过本发明制备的黄连解毒散主治三焦湿热、疮黄肿毒,广泛应用于马、牛、羊、猪及家禽,该散剂对多种细菌均有抑制作用,可用于肺炎、流行性脑脊髓炎、脓毒血症的治疗,对超广谱β-内酰胺酶及持续高产AmpC酶菌株均有不同程度的抑制作用,其中对金黄色葡萄球菌、表皮葡萄球菌、变形杆菌、痢疾杆菌的抑制作用较强。
作为优选,所述中草药添加剂由以下成分及重量份组成:红心果固体原料60-80份、金龙胆草1-6份、板蓝根5-15份、金银花2-8份、首乌藤13-17份,红心果固体原料包括红心果5-10重量份和其他固体原料0.5-5重量份,其它固体原料为玉米粉、小麦粉、豆粕粉中的一种或多种。
该种中草药添加剂有效降低禽类淘汰率和死亡率,降低免疫应激,提高禽类免疫效果,还具有降血压、广谱的抗菌、抗病毒的作用。
作为优选,所述活性多肽的氨基酸序列为
HSHACASYYCSKFCGTACAKFCGTASCTHYLSCTHYLGKLCACVNCSR,该活性多肽具有很强的抗氧化性能,能够有效的清除自由基和脂类过氧化物,保护解毒散成分中的大分子和生物膜,可有效防止解毒散中的成分发生氧化,产生霉变,且不影响解毒散的口感。
作为优选,所述甜味剂由以下成分及重量份组成:甜菊糖甙70-100份、甜蜜素15-35份、糖精钠15-30份、氯贝丁酯0.3-0.5份、单不饱和脂肪酸0.1-1份、糊精5-10份和香精0.3-1.5份,甜味剂可避免肥胖、高血压、糖尿病等疾病问题,还可缓和黄连解毒散的苦味,利于禽类食用,且甜味剂还具有优异下降血压功能,可提升养殖禽类的品质。
作为优选,所述步骤1)中,中草药微粉中需粉碎至200目过筛。
确保中草药的发挥最佳药效。
作为优选,所述步骤3)中,所述步骤2)中,pH值要求为4.8。
通过固化处理有利于将解毒散中的各成分结合。
作为优选,所述步骤3)中,在50℃下酶解三小时。
作为优选,所述步骤4)中固化后的混合物经真空干燥,粉碎至4-8目,过筛,通过真空干燥去除解毒散中的水分利于保存,通过粉碎有利于禽类食用,消化吸收。
与现有技术相比,本发明的有益效果为:本发明增加了酶提取步骤,可以大大提高其提取率,在相同组分、配比和工艺条件下,制备的黄连解毒散份量多;本发明采用酶辅助提取中药材中组分能使细胞中成分向周围环境扩散的阻力减小,提高提取率,缩短提取时间,同时能保持天然产物的立体构型及生物活性。
本发明制备的黄连解毒散主治三焦湿热、疮黄肿毒,广泛应用于马、牛、羊、猪及家禽,该散剂对多种细菌均有抑制作用,可用于肺炎、流行性脑脊髓炎、脓毒血症的治疗,对超广谱β-内酰胺酶及持续高产AmpC酶菌株均有不同程度的抑制作用,其中对金黄色葡萄球菌、表皮葡萄球菌、变形杆菌、痢疾杆菌的抑制作用较强,添加的中草药添加剂有效降低禽类淘汰率和死亡率,降低免疫应激,提高禽类免疫效果,还具有降血压、广谱的抗菌、抗病毒的作用,添加的甜味剂可避免肥胖、高血压、糖尿病等疾病问题,还可缓和黄连解毒散的苦味,利于禽类食用,且甜味剂还具有优异下降血压功能,可提升养殖禽类的品质,本发明的黄连解毒散的抗菌作用机制可能与抑制细菌的DNA合成有关,具有泻火解毒之功效。
具体实施方式
以下结合实施例对本发明作进一步详细描述:
实施例1
一种酶提取式黄连解毒散制备工艺,包括以下步骤:
1)将黄连、黄芩、黄柏、栀子、大黄、绞股蓝皂苷、甘草粉碎后,中草药微粉中需过在200目筛备用;
2)再将过筛后的药粉加入三倍量的50%乙醇浸泡,同时加入复合酶;
3)将复合酶与中药物混合,在50℃条件下匀速搅拌酶解后并加入中草药添加剂、活性多肽和甜味剂,待溶剂挥发后,在-4℃条件下进行固化;
4)于真空干燥器中干燥后,粉碎至6目、过筛、分装即得黄连解毒散;
黄连解毒散由以下成分及重量份组成:黄连20份、黄芩18份、黄柏22份、绞股蓝皂苷0.4份、栀子8份、大黄12份、甘草11份、中草药添加剂1份、活性多肽0.24份、甜味剂0.6份。
活性多肽的氨基酸序列为HSHACASYYCSKFCGTACAKFCGTASCTHYLSCTHYLGKLCACVNCSR,该活性多肽具有很强的抗氧化性能,能够有效的清除自由基和脂类过氧化物,保护解毒散成分中的大分子和生物膜,可有效防止解毒散中的成分发生氧化,产生霉变,且不影响解毒散的口感。
中草药添加剂由以下成分及重量份组成:红心果固体原料70份、金龙胆草5份、板蓝根6份、金银花6份、首乌藤15份,红心果固体原料包括红心果6重量份和其他固体原料4重量份,其它固体原料为玉米粉、小麦粉、豆粕粉三种混合物,中草药添加剂有效降低禽类淘汰率和死亡率,降低免疫应激,提高禽类免疫效果,还具有降血压、广谱的抗菌、抗病毒的作用。
甜味剂由以下成分及重量份组成:甜菊糖甙80份、甜蜜素22份、糖精钠16份、氯贝丁酯0.35份、单不饱和脂肪酸0.6份、糊精7份和香精1.2份,甜味剂可避免肥胖、高血压、糖尿病等疾病问题,还可缓和黄连解毒散的苦味,利于禽类食用,且甜味剂还具有优异下降血压功能,可提升养殖禽类的品质。
实施例2
一种酶提取式黄连解毒散制备工艺,包括以下步骤:
1)将黄连、黄芩、黄柏、栀子、大黄、绞股蓝皂苷、甘草粉碎后,中草药微粉中需过在200目筛备用;
2)再将过筛后的药粉加入三倍量的50%乙醇浸泡,同时加入复合酶;
3)将复合酶与中药物混合,在50℃条件下匀速搅拌酶解后并加入中草药添加剂、活性多肽和甜味剂,待溶剂挥发后,在-4℃条件下进行固化;
4)于真空干燥器中干燥后,粉碎至5目、过筛、分装即得黄连解毒散;黄连解毒散由以下成分及重量份组成:黄连18份、黄芩12份、黄柏16份、绞股蓝皂苷0.2份、栀子8份、大黄11份、甘草15份、中草药添加剂3份、活性多肽0.23份、甜味剂0.7份。
活性多肽的氨基酸序列为HSHACASYYCSKFCGTACAKFCGTASCTHYLSCTHYLGKLCACVNCSR,该活性多肽具有很强的抗氧化性能,能够有效的清除自由基和脂类过氧化物,保护解毒散成分中的大分子和生物膜,可有效防止解毒散中的成分发生氧化,产生霉变,且不影响解毒散的口感。
中草药添加剂由以下成分及重量份组成:红心果固体原料65份、金龙胆草4份、板蓝根7份、金银花6份、首乌藤15份,红心果固体原料包括红心果6重量份和其他固体原料0.8重量份,其它固体原料为玉米粉、小麦粉两种混合物,中草药添加剂有效降低禽类淘汰率和死亡率,降低免疫应激,提高禽类免疫效果,还具有降血压、广谱的抗菌、抗病毒的作用。
甜味剂由以下成分及重量份组成:甜菊糖甙82份、甜蜜素20份、糖精钠23份、氯贝丁酯0.4份、单不饱和脂肪酸0.6份、糊精7份和香精1.2份,甜味剂可避免肥胖、高血压、糖尿病等疾病问题,还可缓和黄连解毒散的苦味,利于禽类食用,且甜味剂还具有优异下降血压功能,可提升养殖禽类的品质。
实施例3
称取黄连、黄芩、黄柏、栀子、大黄、绞股蓝皂苷、甘草,在200目中药微粉中加入适量50%乙醇将药物浸润,再加入复合酶;将载体与被浸润的药物混合,加热条件下加入中草药添加剂、甜味剂和活性多肽后匀速搅拌一段时间,待溶剂挥发后,迅速置于-4℃使之固化;于真空干燥器中干燥后,粉碎、过5号筛、分装即得;按8%、15%的剂量以及空白对照组分别实验,喂养相同品种的猪,1个月后,结果见下表,
由上表可知,15%添加组中养殖猪的增重率明显高于空白对照组和8%添加组;且15%添加组养殖猪的患病率远远低于空白对照组和8%添加组。
上述实施例中的常规技术为本领域技术人员所知晓的现有技术,例如,真空干燥、固化等常规技术,故在此不再详细描述。
当然,上述说明并非对本发明的限制,本发明也并不限于上述举例,本技术领域的普通技术人员在本发明的实质范围内,作出的变化、改型、添加或替换,也应属于本发明的保护范围。
Claims (8)
1.一种酶提取式黄连解毒散制备工艺,其特征在于:其包括以下步骤:
1)将黄连、黄芩、黄柏、栀子、绞股蓝皂苷、大黄、甘草清洗,晒干,粉碎备用;
2)将纤维素酶和果胶蛋白酶按质量比1:1于醋酸-醋酸钠缓冲液中;
3)加入三倍量的50%乙醇浸泡药材,并加入复合酶溶液,酶解后,减压抽滤得到滤液后,加入中草药添加剂、活性多肽和甜味剂,待溶剂挥发后,进行固化;
4)于真空干燥器中干燥后,粉碎、过筛、分装即得黄连解毒散;
该黄连解毒散由以下成分及重量份组成:黄连15-25份、黄芩10-30份、黄柏10-30份、绞股蓝皂苷0.1-0.5份、栀子5-15份、大黄10-20份、甘草10-20份、中草药添加剂1-3份、活性多肽0.2-0.4份、甜味剂0.5-1份、混合酶5-10份。
2.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述中草药添加剂由以下成分及重量份组成:红心果固体原料60-80份、金龙胆草1-6份、板蓝根5-15份、金银花2-8份、首乌藤13-17份,所述红心果固体原料包括红心果5-10重量份和其他固体原料0.5-5重量份,所述其它固体原料为玉米粉、小麦粉、豆粕粉中的一种或多种。
3.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述活性多肽的氨基酸序列为HSHACASYYCSKFCGTACAKFCGTASCTHYLSCTHYLGKLCACVNCSR。
4.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述甜味剂由以下成分及重量份组成:甜菊糖甙70-100份、甜蜜素15-35份、糖精钠15-30份、氯贝丁酯0.3-0.5份、单不饱和脂肪酸0.1-1份、糊精5-10份和香精0.3-1.5份。
5.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述步骤1)中,中草药微粉中需粉碎至200目过筛。
6.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述步骤2)中,pH值要求为4.8。
7.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述步骤3)中,在50℃下酶解三小时。
8.根据权利要求1所述的酶提取式黄连解毒散制备工艺,其特征在于:所述步骤4)中固化后的混合物经真空干燥,粉碎至4-8目,过筛。
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