CN112578129A - ABO positive and negative typing/RhD blood type detection micro-disc - Google Patents
ABO positive and negative typing/RhD blood type detection micro-disc Download PDFInfo
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- CN112578129A CN112578129A CN202011406655.0A CN202011406655A CN112578129A CN 112578129 A CN112578129 A CN 112578129A CN 202011406655 A CN202011406655 A CN 202011406655A CN 112578129 A CN112578129 A CN 112578129A
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses an ABO positive and negative shaping/RhD blood type detection microdisk, which comprises a chassis, wherein a blank contrast area, an ABO negative shaping detection area, an A/B type positive shaping detection area and a RhD detection area are arranged on the chassis; the chassis is provided with first detecting channel and second detecting channel in the position that lies in the blank contrast district, and the position that lies in the anti-type of ABO detection zone is provided with third detecting channel, fourth detecting channel and fifth detecting channel, and the position that lies in the positive type of A/B detection zone is provided with sixth detecting channel and seventh detecting channel, and the position that lies in the RhD detection zone is provided with eighth detecting channel. The micro-disc can realize that ABO positive and negative sizing/RhD detection can be completed on one detection device at one time, and the purposes of improving detection flux, facilitating operation and tracing detection results are realized. Meanwhile, the detection cost is reduced, and medical care resources and cost are saved.
Description
Technical Field
The invention belongs to the technical field of biological detection, and particularly relates to an ABO positive and negative typing/RhD blood type detection microdisk.
Background
Blood is the most important living substance of human body, and blood cells and serum components contained in the blood have the functions of transportation, immune protection, metabolic regulation and the like of the living substance of the human body. Blood transfusion is an important means for treating some diseases, rescuing the lives of wounded persons and ensuring the smooth proceeding of some treatments, such as operations. However, if the blood transfusion is improper or wrong, the health of the patient can be seriously damaged, and even death can be caused. In order to ensure the safety of blood transfusion and improve the effect of blood transfusion, the principle of blood transfusion, i.e. safety, efficiency and economy, must be observed. The blood type and the transfusion safety are of great relation and have important clinical significance. To date, scientists have found that over twenty blood group systems, over 400 blood group antigens exist in the blood as red blood cells, white blood cells, platelets and even plasma proteins. The ABO and Rh blood group systems are the two major blood group antigen-antibody systems of red blood cells and are most closely related to blood transfusion, and the reactions of blood transfusions with an ABO/Rh blood group incompatibility are often life threatening.
Blood type is not only important in blood transfusion, but also has application value in anthropology, genetics, forensic science, transplantation immunity, disease resistance (or susceptibility), etc., before blood transfusion, the blood types of patients (recipients) and transfusion persons (donors) must be checked, and cross matching tests are performed. The discovery of blood type opens up new subjects of immunohematology, immunogenetics and the like, and has very important significance for clinical blood transfusion work. In the aspect of clinical blood transfusion, when the circulating blood volume is insufficient or the blood loss is great or the anemia needs to be treated by blood transfusion, blood donors with the same blood type are selected before blood transfusion, then cross matching is carried out, and blood transfusion can be carried out after the blood donors are completely the same; selecting donors with the same blood type when organ transplantation such as skin transplantation, kidney transplantation and the like is carried out; the analysis of the causes of infertility and neonatal hemolysis also deals with the analysis of blood types; blood type knowledge and related techniques are also required in paternity testing.
Safe blood transfusion is a prerequisite for transfusion therapy, otherwise serious damage to the body of the recipient can occur. The blood compatibility test of the blood recipient and the blood donor is a necessary condition for ensuring the safety of clinical blood transfusion and is an important blood examination item. And (4) blood transfusion compatibility detection, wherein the main detection contents comprise blood type verification and cross matching. Blood typing comprises: 1) detecting the type of the blood group antigen on the surface of the red blood cells by using the serum with known antibodies (positive typing), 2) detecting whether the antibodies corresponding to the red blood cell antigens exist in the serum by using the red blood cells with known blood group (negative typing); cross matching is a direct observation of the compatibility of the recipient's serum with the donor's red blood cells (primary matching), the recipient's red blood cells with the donor's plasma (secondary matching). It can be said that the blood transfusion compatibility test is actually to detect the immunoreaction of the erythrocyte blood group antigen with its specific antibody, and can be accomplished by blood group serology test techniques and related reagents.
Blood type testing can be accomplished manually, such as by slide, paper card, or by using commercially available blood type test kits in conjunction with suitable instruments (centrifuges, etc.). Compared with manual operation, the blood type detection kit has the advantages that the operation process is standardized, the detection result is traceable, and the detection result is more reliable. The microplate solid phase agglutination test and the microcolumn gel immunoassay technology are beginning to appear in the last 90 years, and the microplate method and the microcolumn gel method which are widely used in the current automatic operation are developed on the basis of the microplate solid phase agglutination test and the microcolumn gel immunoassay technology and become the main methods for blood type detection in departments such as the clinical laboratory of the three hospitals, the central blood station and the like. The microplate method and the microcolumn gel method can be operated automatically, but have very high requirements on the quality of a sample to be detected, a reagent and an instrument, long detection period time, complicated operation steps, requirements on technical training and the like, high material cost (accounting for about 40% of detection cost), unsuitability for wide popularization and application and huge economic burden of a medical health care system.
Disclosure of Invention
The technical problem to be solved by the present invention is to provide a micro disc for ABO positive and negative typing/RhD blood type detection, which is designed to overcome the shortcomings of the prior art. The microdisk can finish ABO positive and negative sizing/RhD blood type verification on one detection device at one time, and finally achieves the purposes of improving detection flux, facilitating operation and tracing a detection result. Meanwhile, the detection cost is reduced, and medical care resources and cost are saved.
In order to solve the technical problems, the invention adopts the technical scheme that: an ABO positive and negative shaping/RhD blood type detection microdisk is characterized by comprising a chassis, wherein a blank contrast area, an ABO negative shaping detection area, an A/B type positive shaping detection area and a RhD detection area are arranged on the chassis; a first detection channel and a second detection channel are arranged at the part of the chassis, which is positioned in the blank control area, and quality control test strips (the rest components still exist and do not contain antigen or antibody test strips) are respectively arranged in the first detection channel and the second detection channel; a third detection channel, a fourth detection channel and a fifth detection channel are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis, a test strip coated with an A antigen is placed in the third detection channel, a test strip coated with a B antigen is placed in the fourth detection channel, and a test strip coated with an H antigen is placed in the fifth detection channel; a sixth detection channel and a seventh detection channel are arranged at the part, located in the A/B type positive sizing detection area, of the chassis, a test strip coated with an A antibody is placed in the sixth detection channel, and a test strip coated with a B antibody is placed in the seventh detection channel; and an eighth detection channel is arranged at the position, located in the RhD detection zone, on the chassis, and a test strip coated with a D antibody is placed in the eighth detection channel.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that 8 detection channels on the chassis are uniformly distributed around the center of the chassis by taking the center of the chassis as the center of a circle.
Foretell little dish of ABO positive and negative design rhD blood type detection, its characterized in that still includes the shrouding disc with chassis matched with, be provided with the color development window corresponding with every detection channel on the shrouding disc, locate at the chassis center on the shrouding disc and seted up the sample addition mouth directly over.
The ABO positive and negative typing/RhD blood type detection micro-disc is characterized in that the quality control test strip, the test strip coated with the A antigen, the test strip coated with the B antigen, the test strip coated with the H antigen, the test strip coated with the A antibody, the test strip coated with the B antibody and the test strip coated with the D antibody respectively comprise a non-absorbent bottom plate, a sample pad, a combination pad, a chromatographic pad and a chromogenic pad, wherein the sample pad, the combination pad, the chromatographic pad and the chromogenic pad are sequentially arranged on the bottom plate, the lower edge of the combination pad is in lap joint with the upper edge of the sample pad, the upper edge of the combination pad is in lap joint with one end of the chromatographic pad, and the chromatographic pad is in lap joint with the other end of.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that anticoagulant is laid on the sample pad.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that a chemical color developing agent is coated on the color developing pad.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that the chemical color developing agent is bromocresol green.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that a binding pad of the test strip coated with the A antigen is coated with the A antigen marked by a marker, and a chromatography pad of the test strip coated with the A antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that the marker is colloidal gold particles, fluorescent microsphere particles or latex particles.
The ABO positive and negative typing/RhD blood type detection microdisk is characterized in that the chromatography pad is made of nitrocellulose, the combination pad is made of glass cellulose or polyester fiber, and the color development pad is made of polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
Compared with the prior art, the invention has the following advantages:
1. the detection micro-disc can finish ABO positive and negative typing/RhD blood type verification on one detection device at one time, and finally achieves the purposes of improving detection flux, facilitating operation and tracing detection results. Meanwhile, the multi-channel detection reduces the detection cost and saves the medical insurance resources and the cost.
2. The detection micro-disc can finish the combined detection of positive and negative typing of a plurality of blood types in a short time, is simple, convenient and quick to operate, particularly adds a dyeing indicator which can react with an antibody to generate obvious color change in a color development area of a color development window, has intuitive and identifiable results, has low requirements on professional technical level of operators, and is suitable for clinical detection and blood type detection requirements of emergency situations.
3. The blood type detection micro-disc has low cost, antigen and antibody reagent required by reaction is pre-coated, the micro-disc is stored at room temperature, is convenient to carry and has long storage period.
4. The detection process of the micro-disk is simple and convenient to operate, and the detection result is only required to be read for 2-5 minutes; special auxiliary instruments and equipment and detection reagents are not required to be equipped, and the reaction result can be used for judging a color development result through visual color.
The technical solution of the present invention is further described in detail below with reference to the accompanying drawings and examples.
Drawings
Fig. 1 is a schematic structural view of the chassis of the present invention.
Fig. 2 is a schematic structural diagram of the cover tray of the present invention.
FIG. 3 is a schematic view of a structure of the test strip placed on the chassis of the present invention.
FIG. 4 is a schematic structural diagram of the quality control test strip, the test strip coated with the antibody A, the test strip coated with the antibody B and the test strip coated with the antibody D of the present invention.
FIG. 5 is a schematic structural diagram of the test strip coated with the A antigen, the test strip coated with the B antigen and the test strip coated with the H antigen.
Description of reference numerals:
1-a chassis; 2-cover disc; 3-a first detection channel;
4-a second detection channel; 5-a third detection channel; 6-fourth detection channel;
7-fifth detection channel; 8-sixth detection channel; 9-a seventh detection channel;
10-eighth detection channel; 11-a base plate; 12 — sample pad;
13-a conjugate pad; 14-a chromatographic pad; 15-color pad;
16-sample addition port.
Detailed Description
The antigen and antibody sources related to the invention are as follows:
antigen: antigen A, purchased from Changchun Border Biotechnology, Inc., under the product name substance A; antigen B, available from Changchun Border Biotechnology, Inc., under the product name substance B; h antigen, ELICITYL-OLIGOTECH, France.
Antibody: antibody A (anti-blood group A monoclonal antibody), purchased from Shanghai blood biomedicine, LLC; antibody B (anti-blood group B monoclonal antibody), purchased from Shanghai blood biomedicine, LLC; antibody D (RhD monoclonal antibody), available from Shanghai blood biomedicine, LLC; rabbit anti-human IgM antibody, purchased from abcam.
As shown in fig. 1 to 5, the ABO positive and negative typing/RhD blood type detecting microdisk of the present invention comprises a chassis 1, wherein a blank control area, an ABO negative typing detection area, an a/B type positive typing detection area, and a RhD detection area are disposed on the chassis 1; a first detection channel 3 and a second detection channel 4 are arranged at the position of the blank control area on the chassis 1, and quality control test strips (the rest components still exist and do not contain antigen or antibody test strips) are placed in the first detection channel 3 and the second detection channel 4; a third detection channel 5, a fourth detection channel 6 and a fifth detection channel 9 are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis 1, a test strip coated with an A antigen is placed in the third detection channel 5, a test strip coated with a B antigen is placed in the fourth detection channel 6, and a test strip coated with an H antigen is placed in the fifth detection channel 9; a sixth detection channel 7 and a seventh detection channel 8 are arranged at the position, located in the A/B type positive sizing detection area, on the chassis 1, a test strip coated with an A antibody is placed in the sixth detection channel 7, and a test strip coated with a B antibody is placed in the seventh detection channel 8; an eighth detection channel 10 is arranged at a position, located in the RhD detection area, on the chassis 1, and a test strip coated with a D antibody is placed in the eighth detection channel 10.
In this embodiment, the 8 detection channels on the chassis 1 are uniformly distributed around the center of the chassis 1.
In this embodiment, the device further comprises a cover plate 2 matched with the chassis 1, the cover plate 2 is provided with a color development window corresponding to each detection channel, and the cover plate 2 is provided with a sample injection port 16 right above the center of the chassis 1.
In this embodiment, the quality control test strip, the test strip coated with the antigen a, the test strip coated with the antigen B, the test strip coated with the antigen H, the test strip coated with the antibody a, the test strip coated with the antibody B, and the test strip coated with the antibody D all include a non-absorbent bottom plate 11, and a sample pad 12, a combination pad 13, a chromatographic pad 14, and a color development pad 15 that are sequentially disposed on the bottom plate 11, wherein a lower edge of the combination pad 13 is overlapped with an upper edge of the sample pad 12, an upper edge of the combination pad 13 is overlapped with one end of the chromatographic pad 14, and the other end of the chromatographic pad 14 is overlapped with the color development pad 15.
In this embodiment, an anticoagulant is spread on the sample pad 12.
In this embodiment, the color pad 15 is coated with a chemical developer.
In this embodiment, the chemical color-developing agent is bromocresol green.
In this embodiment, the test strip coated with the a antigen has the a antigen labeled with a marker coated on the conjugate pad, and the IgM antibody strip is disposed on the chromatography pad of the test strip coated with the a antigen; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
In this embodiment, the label is a colloidal gold particle, a fluorescent microsphere particle, or a latex particle.
In this embodiment, the material of the chromatographic pad 14 is nitrocellulose, the material of the bonding pad 13 is glass cellulose or polyester fiber, and the material of the color developing pad 15 is polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
The preparation method of the test strip and the detection micro-disk comprises the following steps:
1. preparing a chemical color developing agent solution: adding 0.01g of bromocresol green powder, 2.0mL of sodium citrate solution (0.01g/mL), 5% of gelatin and 0.002g of sodium azide (or proclin300) into 85mL of purified water, stirring until the solution is completely dissolved, adjusting the pH value of the solution to 4.0 by using HCl solution, transferring the solution into a 100mL volumetric flask, adding purified water, flushing a solution preparation container, transferring the solution into the volumetric flask to a scribed line, uniformly mixing, standing, and storing in a sealed polyethylene bottle for later use.
2. Preparing a color developing pad: selecting a piece of polyester fiber (or cotton velvet paper) with the specification of 300mm x 200mm, putting the polyester fiber (or cotton velvet paper) into a square box containing 100mL of PBS solution, processing the polyester fiber (or cotton velvet paper) on a circumferential shaking bed at 100rpm for 20min, and after the processing, putting the polyester fiber (or cotton velvet paper) into a 50 ℃ oven to dry for 2h and then taking out the polyester fiber (or cotton velvet paper); cutting the dried polyester fiber or cotton linter paper) into strips of 300mm by 7mm, putting 20 strips into a square box filled with 100mL of dye indicator solution for dip-dyeing, putting the square box on a circumference shaking table at 100rpm for shaking and oscillating for 1h in the dip-dyeing process to ensure that the strips are uniformly dip-dyed, and after the dip-dyeing is finished, obliquely taking out the strips from the sides of the two sides by using tweezers, putting the strips on a smooth plate, and drying the strips in a drying oven at 37 ℃ for 5 h.
3. Immobilization of antigen antibodies
A. Preparation of antibody-coated conjugate pad: a. taking a glass cellulose membrane (or polyester fiber), soaking in a PBS buffer solution for 30min, taking out, and putting into an oven for quick drying; b. further placing the dried glass cellulose membrane (or polyester fiber) into PBS buffer solution added with 0.2% gelatin, treating for 30min at 100rpm on a circular shaker to remove air bubbles, standing for a period of time, and drying in an oven at 37 ℃; c. cutting the glass cellulose membrane (or polyester fiber) after the two treatments into strips with the width of 6mm, respectively spraying an antibody A solution, an antibody B solution and an antibody D solution on the cut strips, drying at 37 ℃ for 5h, taking out for later use, and correspondingly obtaining a binding pad coated with the antibody A, a binding pad coated with the antibody B and a binding pad coated with the antibody D.
B. Preparing an IgM antibody strip on the chromatographic pad: soaking the nitrocellulose membrane in PBS buffer solution for 30min, and quickly drying; cutting the dried nitrocellulose membrane into strips of 10mm, spreading PBS buffer solution containing 0.2% gelatin on the cut strips, standing for 3-5 min, and drying in an oven at 37 ℃; taking out the strip, selecting IgM antibody, and scribing with the scribing concentration of 5 μ L/cm and the scribing width of 4 mm; and (3) after the scribing is finished, putting the obtained product into an oven to be dried for 6h at the temperature of 37 ℃, and taking the obtained product out for later use to obtain the chromatography pad provided with the IgM antibody strip.
C. Preparation of labeled antigen: taking colloidal gold particles (fluorescent microsphere particles or latex particles can also be adopted) with the particle diameter of 30 nm-50 nm, and adjusting the pH value to 7.8 by using 0.1mol/L potassium carbonate solution; respectively adding the antigen A, the antigen B or the antigen H on a magnetic stirrer at a low speed while stirring, continuously stirring for 30min, adding the PEG solution, and stirring for 1H to ensure that the final concentration is 0.05%. After the stirring was completed, the resulting labeling solution was centrifuged at 12000rpm for 10min using a refrigerated centrifuge. Suspending the precipitate to 1.0mL with 0.9% NaCl solution, and storing at 4 deg.C; the corresponding labeled a antigen, labeled B antigen or labeled H antigen.
D. Preparation of antigen-coated conjugate pad: placing a 300mm by 200mm glass cellulose membrane (or polyester fiber) into a square box containing 100mL of 0.9% NaCl solution, placing the square box on a circular shaking bed for processing at 100rpm for 30min, taking out the square box, and placing the square box in an oven at 50 ℃ for drying; cutting the dried glass cellulose membrane (or polyester fiber) into strips with the width of 6mm, respectively and uniformly dispersing the obtained labeled A antigen, labeled B antigen and labeled H antigen on the strips with the width of 6mm, drying at 37 ℃ for 6H, and taking out for later use.
4. Assembly
Assembling the test strip: the test paper strips of the ABO reverse sizing detection zone, the A/B type positive sizing detection zone and the RhD detection zone are all composed of a PVC bottom plate, a sample pad, a combination pad, a chromatography pad and a color development pad; and a sample pad and a binding pad which is pre-fixed with antibody/antigen are sequentially paved on the upper surface of the PVC plate along the chromatography direction. Wherein, the sample pad of the test strip in the A/B type positive shaping detection area and the RhD detection area is glass cellulose membrane RB45, and the sample pad of the test strip in the ABO reverse shaping detection area is GF 2. Cutting the sample pad into strips with the width of 6mm, pressing the combination pad for 1mm according to the sample pad, pressing the chromatography pad for 1mm according to the combination pad, pressing the chromatography pad for 1mm according to the color development pad for 1mm, and attaching the strips prepared and corresponding to each part to a PVC bottom plate with the width of 3cm to assemble a large plate. The blank control area is assembled by using a blank combination pad without any antigen-antibody fixed combination pad, wherein other parts of one quality control test strip are consistent with the test strip of the A/B type positive shaping detection area, and other parts of the other quality control test strip are consistent with the test strip of the ABO reverse shaping detection area. The various assembled large plates were cut into test strips according to a width of 4 mm.
Assembling the microdisk: and installing the cut test strip in a corresponding detection channel of the chassis according to the corresponding position, and installing the cover disc on the chassis to enable the color development window of the cover disc to correspond to the corresponding detection channel. Such as: the test strip coated with the antibody A corresponds to A window F-A, the test strip coated with the antibody B corresponds to A window F-B, the test strip coated with the antigen B corresponds to A window R-B, the test strip coated with the antigen A corresponds to A window R-A, the two quality control test strips correspond to A window F-QC and A window R-QC respectively, the test strip coated with the antigen H corresponds to A window R-O, and the test strip coated with the antibody D corresponds to A window F-D.
The use method of the detection microdisk comprises the following steps:
taking 0.4mL of blood sample to be detected, adding the blood sample into the sample adding hole 16 of the detection micro-disc, after the blood sample is fully contacted with the sample pad of each detection channel test strip (about after no blood sample exists at the bottom of the sample adding hole), dropwise adding 80 mu L of 0.9% NaCl solution, and observing the color change of the color development window for 3-5 minutes. And judging the identification result according to the color change of the color development window.
The results were evaluated in accordance with Table 1.
Table 1 results judgment reference table
105 blood samples were measured using the test microplate according to the present invention in accordance with the above-mentioned method of use, and the results of the measurements are shown in Table 2, using the conventional slide method and the tube method as controls.
TABLE 2 statistical table for blood grouping
As can be seen from Table 2, the ABO positive and negative sizing/RhD detection can be completed by one-step use of the detection microdisk provided by the invention, the detection result is reliable, and the coincidence rate reaches 100%.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, changes and equivalent structural changes made to the above embodiment according to the technical spirit of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. An ABO positive and negative typing/RhD blood type detection microdisk is characterized by comprising a chassis (1), wherein a blank contrast area, an ABO negative typing detection area, an A/B type positive typing detection area and a RhD detection area are arranged on the chassis (1); a first detection channel (3) and a second detection channel (4) are arranged at the position, located in the blank control area, of the chassis (1), and quality control test strips are placed in the first detection channel (3) and the second detection channel (4); a third detection channel (5), a fourth detection channel (6) and a fifth detection channel (9) are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis (1), a test strip coated with an A antigen is placed in the third detection channel (5), a test strip coated with a B antigen is placed in the fourth detection channel (6), and a test strip coated with an H antigen is placed in the fifth detection channel (9); a sixth detection channel (7) and a seventh detection channel (8) are arranged at the position, located in the A/B type positive sizing detection area, on the chassis (1), a test strip coated with an A antibody is placed in the sixth detection channel (7), and a test strip coated with a B antibody is placed in the seventh detection channel (8); the chassis (1) is provided with eighth detection channel (10) in the position that is located the RhD detection zone, places the test paper strip that the coating has the D antibody in eighth detection channel (10).
2. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 1, wherein 8 detecting channels on the chassis (1) are centered around the center of the chassis (1) and evenly distributed around the center.
3. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 1, further comprising a cover plate (2) matched with the chassis (1), wherein the cover plate (2) is provided with a color developing window corresponding to each detecting channel, and a sample adding port (16) is arranged on the cover plate (2) and positioned right above the center of the chassis (1).
4. The ABO positive and negative typing/RhD blood type detection microdisk of claim 1, wherein the quality control test strip, the test strip coated with the A antigen, the test strip coated with the B antigen, the test strip coated with the H antigen, the test strip coated with the A antibody, the test strip coated with the B antibody and the test strip coated with the D antibody all comprise a non-absorbent bottom plate (11), and a sample pad (12), a binding pad (13), a chromatographic pad (14) and a chromogenic pad (15) which are sequentially arranged on the bottom plate (11), wherein the lower edge of the binding pad (13) is lapped with the upper edge of the sample pad (12), the upper edge of the binding pad (13) is lapped with one end of the chromatographic pad (14), and the other end of the chromatographic pad (14) is lapped with the chromogenic pad (15).
5. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 4, wherein anticoagulant is spread on the sample pad (12).
6. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 4, wherein said color developing pad (15) is coated with chemical color developing agent.
7. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 6, wherein said chemical color developing agent is bromocresol green.
8. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 4, wherein the A antigen marked by the marker is coated on the bonding pad of the test strip coated with the A antigen, and the IgM antibody strip is arranged on the chromatographic pad of the test strip coated with the A antigen; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
9. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 8, wherein said label is colloidal gold particle, fluorescent microsphere particle or latex particle.
10. The ABO positive and negative typing/RhD blood type detecting microdisk of claim 8, wherein the chromatography pad (14) is made of nitrocellulose, the combination pad (13) is made of glass cellulose or polyester fiber, and the color developing pad (15) is made of polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
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