CN213715240U - Blood type detection microdisk - Google Patents
Blood type detection microdisk Download PDFInfo
- Publication number
- CN213715240U CN213715240U CN202022900110.7U CN202022900110U CN213715240U CN 213715240 U CN213715240 U CN 213715240U CN 202022900110 U CN202022900110 U CN 202022900110U CN 213715240 U CN213715240 U CN 213715240U
- Authority
- CN
- China
- Prior art keywords
- detection
- pad
- test strip
- coated
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a blood type detection micro-disc, which comprises a chassis and a cover disc matched with the chassis, wherein a blank contrast area, an ABO reverse sizing detection area, an A/B type positive sizing detection area and an RhD detection area are arranged on the chassis; the chassis is provided with a blank control area, and is characterized in that a first detection channel and a second detection channel are arranged at the position of the chassis in the blank control area, a third detection channel, a fourth detection channel and a fifth detection channel are arranged at the position of the chassis in the ABO type reverse sizing detection area, a sixth detection channel and a seventh detection channel are arranged at the position of the chassis in the A/B type normal sizing detection area, and an eighth detection channel is arranged at the position of the chassis in the RhD detection area. The micro-disc can realize that ABO positive and negative sizing/RhD detection can be completed on one detection device at one time, and the purposes of improving detection flux, facilitating operation and tracing detection results are realized. Meanwhile, the detection cost is reduced, and medical care resources and cost are saved.
Description
Technical Field
The utility model belongs to the technical field of biological detection, concretely relates to blood type detects minidisk.
Background
Blood is the most important living substance of human body, and blood cells and serum components contained in the blood have the functions of transportation, immune protection, metabolic regulation and the like of the living substance of the human body. Blood transfusion is an important means for treating some diseases, rescuing the lives of wounded persons and ensuring the smooth proceeding of some treatments, such as operations. However, if the blood transfusion is improper or wrong, the health of the patient can be seriously damaged, and even death can be caused. In order to ensure the safety of blood transfusion and improve the effect of blood transfusion, the principle of blood transfusion, i.e. safety, efficiency and economy, must be observed. The blood type and the transfusion safety are of great relation and have important clinical significance. To date, scientists have found that over twenty blood group systems, over 400 blood group antigens exist in the blood as red blood cells, white blood cells, platelets and even plasma proteins. The ABO and Rh blood group systems are the two major blood group antigen-antibody systems of red blood cells and are most closely related to blood transfusion, and the reactions of blood transfusions with an ABO/Rh blood group incompatibility are often life threatening.
Blood type is not only important in blood transfusion, but also has application value in anthropology, genetics, forensic science, transplantation immunity, disease resistance (or susceptibility), etc., before blood transfusion, the blood types of patients (recipients) and transfusion persons (donors) must be checked, and cross matching tests are performed. The discovery of blood type opens up new subjects of immunohematology, immunogenetics and the like, and has very important significance for clinical blood transfusion work. In the aspect of clinical blood transfusion, when the circulating blood volume is insufficient or the blood loss is great or the anemia needs to be treated by blood transfusion, blood donors with the same blood type are selected before blood transfusion, then cross matching is carried out, and blood transfusion can be carried out after the blood donors are completely the same; selecting donors with the same blood type when organ transplantation such as skin transplantation, kidney transplantation and the like is carried out; the analysis of the causes of infertility and neonatal hemolysis also deals with the analysis of blood types; blood type knowledge and related techniques are also required in paternity testing.
Safe blood transfusion is a prerequisite for transfusion therapy, otherwise serious damage to the body of the recipient can occur. The blood compatibility test of the blood recipient and the blood donor is a necessary condition for ensuring the safety of clinical blood transfusion and is an important blood examination item. And (4) blood transfusion compatibility detection, wherein the main detection contents comprise blood type verification and cross matching. Blood typing comprises: 1) detecting the type of the blood group antigen on the surface of the red blood cells by using the serum with known antibodies (positive typing), 2) detecting whether the antibodies corresponding to the red blood cell antigens exist in the serum by using the red blood cells with known blood group (negative typing); cross matching is a direct observation of the compatibility of the recipient's serum with the donor's red blood cells (primary matching), the recipient's red blood cells with the donor's plasma (secondary matching). It can be said that the blood transfusion compatibility test is actually to detect the immunoreaction of the erythrocyte blood group antigen with its specific antibody, and can be accomplished by blood group serology test techniques and related reagents.
Blood type testing can be accomplished manually, such as by slide, paper card, or by using commercially available blood type test kits in conjunction with suitable instruments (centrifuges, etc.). Compared with manual operation, the blood type detection kit has the advantages that the operation process is standardized, the detection result is traceable, and the detection result is more reliable. The microplate solid phase agglutination test and the microcolumn gel immunoassay technology are beginning to appear in the last 90 years, and the microplate method and the microcolumn gel method which are widely used in the current automatic operation are developed on the basis of the microplate solid phase agglutination test and the microcolumn gel immunoassay technology and become the main methods for blood type detection in departments such as the clinical laboratory of the three hospitals, the central blood station and the like. The microplate method and the microcolumn gel method can be operated automatically, but have very high requirements on the quality of a sample to be detected, a reagent and an instrument, long detection period time, complicated operation steps, requirements on technical training and the like, high material cost (accounting for about 40% of detection cost), unsuitability for wide popularization and application and huge economic burden of a medical health care system.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to the not enough of above-mentioned prior art, provide a blood type detects microdisk. The microdisk can finish ABO positive and negative sizing/RhD blood type verification on one detection device at one time, and finally achieves the purposes of improving detection flux, facilitating operation and tracing a detection result. Meanwhile, the detection cost is reduced, and medical care resources and cost are saved.
In order to solve the technical problem, the utility model discloses a technical scheme is: a blood type detection microdisk is characterized by comprising a chassis and a cover plate matched with the chassis, wherein a blank contrast area, an ABO reverse sizing detection area, an A/B type positive sizing detection area and an RhD detection area are arranged on the chassis; a first detection channel and a second detection channel are arranged at the part of the chassis, which is positioned in the blank control area, and quality control test strips (the rest components still exist and do not contain antigen or antibody test strips) are respectively arranged in the first detection channel and the second detection channel; a third detection channel, a fourth detection channel and a fifth detection channel are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis, a test strip coated with an A antigen is placed in the third detection channel, a test strip coated with a B antigen is placed in the fourth detection channel, and a test strip coated with an H antigen is placed in the fifth detection channel; a sixth detection channel and a seventh detection channel are arranged at the part, located in the A/B type positive sizing detection area, of the chassis, a test strip coated with an A antibody is placed in the sixth detection channel, and a test strip coated with a B antibody is placed in the seventh detection channel; and an eighth detection channel is arranged at the position, located in the RhD detection zone, on the chassis, and a test strip coated with a D antibody is placed in the eighth detection channel.
The blood type detection micro-disc is characterized in that the cover disc is provided with a color development window corresponding to each detection channel.
The blood type detection microdisk is characterized in that 8 detection channels are uniformly distributed around the center of the circle by taking the center of the chassis as the center of the circle, and a sample adding port is formed in the cover disc and is positioned right above the center of the chassis.
Foretell blood type detects microdisk, its characterized in that, the test paper strip all includes the bottom plate that does not absorb water to and set gradually sample pad, combination pad, chromatography pad and the color development pad on the bottom plate, the lower limb of combination pad with the top edge overlap joint of sample pad, the top edge and the chromatography pad one end overlap joint of combination pad, the chromatography pad other end overlap joint color development pad.
The blood type detection micro-disc is characterized in that anticoagulant is laid on the sample pad.
The blood type detection micro-disc is characterized in that the color development pad is coated with a chemical color development agent.
The blood type detection microdisk is characterized in that the A antigen marked by a marker is coated on the bonding pad of the test strip coated with the A antigen, and an IgM antibody strip is arranged on the chromatographic pad of the test strip coated with the A antigen; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
The blood type detection microdisk is characterized in that the marker is colloidal gold particles, fluorescent microsphere particles or latex particles.
The blood type detection micro-disc is characterized in that the chromatography pad is made of nitrocellulose, the combination pad is made of glass cellulose or polyester fiber, and the color development pad is made of polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
Compared with the prior art, the utility model has the following advantage:
1. the utility model discloses a detect microdisk can realize accomplishing the positive and negative design of ABO rhD blood type examination on a detection device last time, finally realize improving and detect the flux, the operation of being convenient for, purpose that the inspection result is traceable. Meanwhile, the multi-channel detection reduces the detection cost and saves the medical insurance resources and the cost.
2. The utility model discloses a detect microdisk can accomplish the joint detection of the positive and negative design of a plurality of blood types in the short time, and is easy and simple to handle swift, especially add in the color development district of color development window can produce the dyeing indicator that obviously discolours with the antibody reaction, and the result is directly perceived can, requires lowly to operating personnel professional technical level, is suitable for clinical examination and emergency situation's blood type detection demand.
3. The detection micro-disc of the utility model is simple and rapid to operate, and the reading of the detection result only needs 2 to 5 minutes; special auxiliary instruments and equipment are not required, and the cost is low; the product is stored at room temperature and is convenient to carry; the detection type is comprehensive, a fresh red blood cell reagent is not needed, and the color development result can be judged through visual color.
The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and embodiments.
Drawings
Fig. 1 is a schematic structural diagram of the chassis of the present invention.
Fig. 2 is a schematic structural diagram of the cover tray of the present invention.
Fig. 3 is a schematic structural diagram of the test strip placed on the chassis of the utility model.
Fig. 4 is the structure schematic diagram of the quality control test strip, the test strip coated with the antibody a, the test strip coated with the antibody B, and the test strip coated with the antibody D of the present invention.
Fig. 5 is a schematic structural diagram of the test strip coated with the antigen a, the test strip coated with the antigen B, and the test strip coated with the antigen H of the present invention.
Description of reference numerals:
1-a chassis; 2-cover disc; 3-a first detection channel;
4-a second detection channel; 5-a third detection channel; 6-fourth detection channel;
7-fifth detection channel; 8-sixth detection channel; 9-a seventh detection channel;
10-eighth detection channel; 11-a base plate; 12 — sample pad;
13-a conjugate pad; 14-a chromatographic pad; 15-color pad;
16-sample addition port.
Detailed Description
The antigen and antibody source of the utility model is as follows:
antigen: antigen A, purchased from Changchun Border Biotechnology, Inc., under the product name substance A; antigen B, available from Changchun Border Biotechnology, Inc., under the product name substance B; h antigen, ELICITYL-OLIGOTECH, France.
Antibody: antibody A (anti-blood group A monoclonal antibody), purchased from Shanghai blood biomedicine, LLC; antibody B (anti-blood group B monoclonal antibody), purchased from Shanghai blood biomedicine, LLC; antibody D (RhD monoclonal antibody), available from Shanghai blood biomedicine, LLC; rabbit anti-human IgM antibody, purchased from abcam.
As shown in fig. 1 to 5, the blood type detecting microdisk of the present invention comprises a chassis 1 and a cover plate 2 matched with the chassis 1, wherein the chassis 1 is provided with a blank contrast area, an ABO reverse sizing detection area, an a/B type normal sizing detection area and an RhD detection area; a first detection channel 3 and a second detection channel 4 are arranged at the position of the blank control area on the chassis 1, and quality control test strips (the rest components still exist and do not contain antigen or antibody test strips) are placed in the first detection channel 3 and the second detection channel 4; a third detection channel 5, a fourth detection channel 6 and a fifth detection channel 9 are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis 1, a test strip coated with an A antigen is placed in the third detection channel 5, a test strip coated with a B antigen is placed in the fourth detection channel 6, and a test strip coated with an H antigen is placed in the fifth detection channel 9; a sixth detection channel 7 and a seventh detection channel 8 are arranged at the position, located in the A/B type positive sizing detection area, on the chassis 1, a test strip coated with an A antibody is placed in the sixth detection channel 7, and a test strip coated with a B antibody is placed in the seventh detection channel 8; an eighth detection channel 10 is arranged at a position, located in the RhD detection area, on the chassis 1, and a test strip coated with a D antibody is placed in the eighth detection channel 10.
In this embodiment, the cover plate 2 is provided with a color development window corresponding to each detection channel.
In this embodiment, the 8 detection channels are uniformly distributed around the center of the chassis 1, and the sample injection port 16 is disposed on the cover plate 2 and directly above the center of the chassis 1.
In this embodiment, the test paper strip all includes non-absorbent bottom plate 11 to and set gradually sample pad 12, combination pad 13, chromatography pad 14 and color development pad 15 on bottom plate 11, the lower limb of combination pad 13 with the top edge overlap joint of sample pad 12, the top edge and the 14 one end overlap joint of chromatography pad of combination pad 13, the 14 other end overlap joint color development pad 15 of chromatography pad.
In this embodiment, an anticoagulant is spread on the sample pad 12.
In this embodiment, the color pad 15 is coated with a chemical developer.
In this embodiment, the test strip coated with the a antigen has the a antigen labeled with a marker coated on the conjugate pad, and the IgM antibody strip is disposed on the chromatography pad of the test strip coated with the a antigen; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
In this embodiment, the label is a colloidal gold particle, a fluorescent microsphere particle, or a latex particle.
In this embodiment, the material of the chromatographic pad 14 is nitrocellulose, the material of the bonding pad 13 is glass cellulose or polyester fiber, and the material of the color developing pad 15 is polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
The utility model discloses a preparation method of test paper strip and detection microdisk is as follows:
1. preparing a chemical color developing agent solution: adding 0.01g of bromocresol green powder, 2.0mL of sodium citrate solution (0.01g/mL), 5% of gelatin and 0.002g of sodium azide (or proclin300) into 85mL of purified water, stirring until the solution is completely dissolved, adjusting the pH value of the solution to 4.0 by using HCl solution, transferring the solution into a 100mL volumetric flask, adding purified water, flushing a solution preparation container, transferring the solution into the volumetric flask to a scribed line, uniformly mixing, standing, and storing in a sealed polyethylene bottle for later use.
2. Preparing a color developing pad: selecting a piece of polyester fiber (or cotton velvet paper) with the specification of 300mm x 200mm, putting the polyester fiber (or cotton velvet paper) into a square box containing 100mL of PBS solution, processing the polyester fiber (or cotton velvet paper) on a circumferential shaking bed at 100rpm for 20min, and after the processing, putting the polyester fiber (or cotton velvet paper) into a 50 ℃ oven to dry for 2h and then taking out the polyester fiber (or cotton velvet paper); cutting the dried polyester fiber or cotton linter paper) into strips of 300mm by 7mm, putting 20 strips into a square box filled with 100mL of dye indicator solution for dip-dyeing, putting the square box on a circumference shaking table at 100rpm for shaking and oscillating for 1h in the dip-dyeing process to ensure that the strips are uniformly dip-dyed, and after the dip-dyeing is finished, obliquely taking out the strips from the sides of the two sides by using tweezers, putting the strips on a smooth plate, and drying the strips in a drying oven at 37 ℃ for 5 h.
3. Immobilization of antigen antibodies
A. Preparation of antibody-coated conjugate pad: a. taking a glass cellulose membrane (or polyester fiber), soaking in a PBS buffer solution for 30min, taking out, and putting into an oven for quick drying; b. further placing the dried glass cellulose membrane (or polyester fiber) into PBS buffer solution added with 0.2% gelatin, treating for 30min at 100rpm on a circular shaker to remove air bubbles, standing for a period of time, and drying in an oven at 37 ℃; c. cutting the glass cellulose membrane (or polyester fiber) after the two treatments into strips with the width of 6mm, respectively spraying an antibody A solution, an antibody B solution and an antibody D solution on the cut strips, drying at 37 ℃ for 5h, taking out for later use, and correspondingly obtaining a binding pad coated with the antibody A, a binding pad coated with the antibody B and a binding pad coated with the antibody D.
B. Preparing an IgM antibody strip on the chromatographic pad: soaking the nitrocellulose membrane in PBS buffer solution for 30min, and quickly drying; cutting the dried nitrocellulose membrane into strips of 10mm, spreading PBS buffer solution containing 0.2% gelatin on the cut strips, standing for 3-5 min, and drying in an oven at 37 ℃; taking out the strip, selecting IgM antibody, and scribing with the scribing concentration of 5 μ L/cm and the scribing width of 4 mm; and (3) after the scribing is finished, putting the obtained product into an oven to be dried for 6h at the temperature of 37 ℃, and taking the obtained product out for later use to obtain the chromatography pad provided with the IgM antibody strip.
C. Preparation of labeled antigen: taking colloidal gold particles (fluorescent microsphere particles or latex particles can also be adopted) with the particle diameter of 30 nm-50 nm, and adjusting the pH value to 7.8 by using 0.1mol/L potassium carbonate solution; respectively adding the antigen A, the antigen B or the antigen H on a magnetic stirrer at a low speed while stirring, continuously stirring for 30min, adding the PEG solution, and stirring for 1H to ensure that the final concentration is 0.05%. After the stirring was completed, the resulting labeling solution was centrifuged at 12000rpm for 10min using a refrigerated centrifuge. Suspending the precipitate to 1.0mL with 0.9% NaCl solution, and storing at 4 deg.C; the corresponding labeled a antigen, labeled B antigen or labeled H antigen.
D. Preparation of antigen-coated conjugate pad: placing a 300mm by 200mm glass cellulose membrane (or polyester fiber) into a square box containing 100mL of 0.9% NaCl solution, placing the square box on a circular shaking bed for processing at 100rpm for 30min, taking out the square box, and placing the square box in an oven at 50 ℃ for drying; cutting the dried glass cellulose membrane (or polyester fiber) into strips with the width of 6mm, respectively and uniformly dispersing the obtained labeled A antigen, labeled B antigen and labeled H antigen on the strips with the width of 6mm, drying at 37 ℃ for 6H, and taking out for later use.
4. Assembly
Assembling the test strip: the test paper strips of the ABO reverse sizing detection zone, the A/B type positive sizing detection zone and the RhD detection zone are all composed of a PVC bottom plate, a sample pad, a combination pad, a chromatography pad and a color development pad; and a sample pad and a binding pad which is pre-fixed with antibody/antigen are sequentially paved on the upper surface of the PVC plate along the chromatography direction. Wherein, the sample pad of the test strip in the A/B type positive shaping detection area and the RhD detection area is glass cellulose membrane RB45, and the sample pad of the test strip in the ABO reverse shaping detection area is GF 2. Cutting the sample pad into strips with the width of 6mm, pressing the combination pad for 1mm according to the sample pad, pressing the chromatography pad for 1mm according to the combination pad, pressing the chromatography pad for 1mm according to the color development pad for 1mm, and attaching the strips prepared and corresponding to each part to a PVC bottom plate with the width of 3cm to assemble a large plate. The blank control area is assembled by using a blank combination pad without any antigen-antibody fixed combination pad, wherein other parts of one quality control test strip are consistent with the test strip of the A/B type positive shaping detection area, and other parts of the other quality control test strip are consistent with the test strip of the ABO reverse shaping detection area. The various assembled large plates were cut into test strips according to a width of 4 mm.
Assembling the microdisk: and installing the cut test strip in a corresponding detection channel of the chassis according to the corresponding position, and installing the cover disc on the chassis to enable the color development window of the cover disc to correspond to the corresponding detection channel. Such as: the test strip coated with the antibody A corresponds to A window F-A, the test strip coated with the antibody B corresponds to A window F-B, the test strip coated with the antigen B corresponds to A window R-B, the test strip coated with the antigen A corresponds to A window R-A, the two quality control test strips correspond to A window F-QC and A window R-QC respectively, the test strip coated with the antigen H corresponds to A window R-O, and the test strip coated with the antibody D corresponds to A window F-D.
The use method of the detection microdisk comprises the following steps:
taking 0.4mL of blood sample to be detected, adding the blood sample into the sample adding hole 16 of the detection micro-disc, after the blood sample is fully contacted with the sample pad of each detection channel test strip (about after no blood sample exists at the bottom of the sample adding hole), dropwise adding 80 mu L of 0.9% NaCl solution, and observing the color change of the color development window for 3-5 minutes. And judging the identification result according to the color change of the color development window.
The results were evaluated in accordance with Table 1.
Table 1 results judgment reference table
105 blood samples were measured using the test microplate according to the present invention in accordance with the above-mentioned method of use, and the results of the measurements are shown in Table 2, using the conventional slide method and the tube method as controls.
TABLE 2 statistical table for blood grouping
As can be seen from Table 2, the ABO positive and negative sizing/RhD detection can be completed by one-step use of the detection microdisk provided by the invention, the detection result is reliable, and the coincidence rate reaches 100%.
The above, only be the utility model discloses a preferred embodiment, it is not right the utility model discloses do any restriction, all according to utility model technical entity to any simple modification, change and the equivalent structure change of doing above embodiment, all still belong to the utility model discloses technical scheme's within the scope of protection.
Claims (9)
1. A minidisc for blood type detection is characterized by comprising a chassis (1) and a cover disc (2) matched with the chassis (1), wherein a blank contrast area, an ABO reverse sizing detection area, an A/B type positive sizing detection area and an RhD detection area are arranged on the chassis (1); a first detection channel (3) and a second detection channel (4) are arranged at the position, located in the blank control area, of the chassis (1), and quality control test strips are placed in the first detection channel (3) and the second detection channel (4); a third detection channel (5), a fourth detection channel (6) and a fifth detection channel (9) are arranged at the position, located in the ABO type reverse sizing detection area, on the chassis (1), a test strip coated with an A antigen is placed in the third detection channel (5), a test strip coated with a B antigen is placed in the fourth detection channel (6), and a test strip coated with an H antigen is placed in the fifth detection channel (9); a sixth detection channel (7) and a seventh detection channel (8) are arranged at the position, located in the A/B type positive sizing detection area, on the chassis (1), a test strip coated with an A antibody is placed in the sixth detection channel (7), and a test strip coated with a B antibody is placed in the seventh detection channel (8); the chassis (1) is provided with eighth detection channel (10) in the position that is located the RhD detection zone, places the test paper strip that the coating has the D antibody in eighth detection channel (10).
2. The micro-disc for blood group testing according to claim 1, wherein the cover disc (2) is provided with a color development window corresponding to each testing channel.
3. The blood type detection microdisk of claim 1, wherein 8 detection channels are uniformly distributed around the center of the bottom disc (1), and the cover disc (2) is provided with a sample injection port (16) right above the center of the bottom disc (1).
4. The blood type detection micro-disc of claim 1, wherein the test strips each comprise a non-absorbent bottom plate (11), and a sample pad (12), a combination pad (13), a chromatographic pad (14) and a chromogenic pad (15) sequentially disposed on the bottom plate (11), wherein the lower edge of the combination pad (13) is overlapped with the upper edge of the sample pad (12), the upper edge of the combination pad (13) is overlapped with one end of the chromatographic pad (14), and the other end of the chromatographic pad (14) is overlapped with the chromogenic pad (15).
5. The micro-disc for blood group testing according to claim 4, wherein said sample pad (12) is coated with anticoagulant.
6. The blood group testing micro-disk of claim 4, wherein said color-developing pad (15) is coated with a chemical color-developing agent.
7. The blood type detection microdisk of claim 4, wherein the test strip coated with the A antigen has a bonding pad coated with the A antigen labeled with a marker, and a chromatography pad coated with the test strip coated with the A antigen has an IgM antibody strip; the binding pad of the test strip coated with the B antigen is coated with the B antigen marked by a marker, and the chromatography pad of the test strip coated with the B antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the antibody A is coated with the antibody A, the binding pad of the test strip coated with the antibody B is coated with the antibody B, the binding pad of the test strip coated with the H antigen is coated with the H antigen, and the chromatography pad of the test strip coated with the H antigen is provided with an IgM antibody strip; the binding pad of the test strip coated with the D antibody is coated with the D antibody.
8. The blood type detection microdisk of claim 7, wherein the label is colloidal gold particles, fluorescent microsphere particles or latex particles.
9. The blood type detection microdisk of claim 7, wherein the chromatography pad (14) is made of nitrocellulose, the combination pad (13) is made of glass cellulose or polyester fiber, and the color development pad (15) is made of polyester fiber or cotton linter paper; the material of the sample pad of the test strip coated with the A antigen and the material of the sample pad of the test strip coated with the B antigen are both glass cellulose or polyester fiber, and the material of the sample pad of the quality control test strip, the material of the sample pad of the test strip coated with the A antibody, the material of the sample pad of the test strip coated with the B antibody, the material of the sample pad of the test strip coated with the H antigen and the material of the sample pad of the test strip coated with the D antibody are both glass fiber or polyester fiber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022900110.7U CN213715240U (en) | 2020-12-05 | 2020-12-05 | Blood type detection microdisk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022900110.7U CN213715240U (en) | 2020-12-05 | 2020-12-05 | Blood type detection microdisk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213715240U true CN213715240U (en) | 2021-07-16 |
Family
ID=76786601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022900110.7U Active CN213715240U (en) | 2020-12-05 | 2020-12-05 | Blood type detection microdisk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213715240U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323835A (en) * | 2021-11-30 | 2022-04-12 | 西安良升生物科技有限公司 | Rapid drug detection kit |
-
2020
- 2020-12-05 CN CN202022900110.7U patent/CN213715240U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323835A (en) * | 2021-11-30 | 2022-04-12 | 西安良升生物科技有限公司 | Rapid drug detection kit |
| CN114323835B (en) * | 2021-11-30 | 2023-08-08 | 西安良升生物科技有限公司 | Drug rapid detection kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104730258B (en) | A kind of Portable blood group system detectio test paper and its detection method | |
| US5169789A (en) | Device and method for self contained solid phase immunodiffusion assay | |
| Seth et al. | Comparison of five blood-typing methods for the feline AB blood group system | |
| JP5181058B2 (en) | Method and kit for rapidly determining human ABO / RH / MN blood type | |
| FI102921B (en) | Method for Preparation of Biologically Active Reagents in Socks from iimide-Containing Polymers, Analytical Element and Methods for Using Them | |
| US20070087451A1 (en) | Immuno-gold lateral flow assay | |
| US20060105402A1 (en) | Blood type method system and device | |
| JPH06273419A (en) | Simple measuring device and method | |
| GB2342443A (en) | Immunoassay device | |
| CN111537720A (en) | Preparation method of novel coronavirus 2019-nCoV IgM/IgG antibody combined detection kit | |
| WO2007081778A2 (en) | Multiplexed detection of anti-red cell alloantibodies | |
| US20050084879A1 (en) | Particle agglutination detection method and device | |
| US20230097619A1 (en) | Automatic test card for multi-blood group system and test method | |
| JPH03170060A (en) | Tester of molecular sample in organic fluid and method of testing prescribed molecule in blood | |
| CN213715240U (en) | Blood type detection microdisk | |
| CN110520736A (en) | A kind of functionalization biology multilayer hole film immune carrier, preparation method, application | |
| CN114686592B (en) | Kit for multi-type target combined detection, preparation method and use method | |
| CN113156143A (en) | Blood group irregular antibody specificity identification method and reagent thereof | |
| CN204719056U (en) | A kind of fast joint detects the kit of HE4 and CA125 | |
| CN112578129A (en) | ABO positive and negative typing/RhD blood type detection micro-disc | |
| CN217688983U (en) | Instant detection card and kit for HLA antibody | |
| Habibi et al. | A Papain‐Bromelin‐Polybrene Four‐Channel Autoanalyzer System for Blood Group Antibody Screening: Analysis of 22,912 Sera | |
| CN109212203A (en) | A kind of quantum dot immune chromatograph test strip of quick detection Brucella antibody | |
| CN110346558B (en) | Use method of detection kit | |
| Jayakumar et al. | Identification and analysis of blood group with digital microscope using image processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |