CN112522214B - 一株高裂解性铜绿假单胞菌噬菌体rdp-pa-20001及其应用 - Google Patents

一株高裂解性铜绿假单胞菌噬菌体rdp-pa-20001及其应用 Download PDF

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CN112522214B
CN112522214B CN202011603159.4A CN202011603159A CN112522214B CN 112522214 B CN112522214 B CN 112522214B CN 202011603159 A CN202011603159 A CN 202011603159A CN 112522214 B CN112522214 B CN 112522214B
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pseudomonas aeruginosa
bacteriophage
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杜新永
李先胜
罗成盛
赵丹丹
王晓铃
张庆
王立坤
刘玉庆
盖春云
马如霞
刘爽
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Rec Union Qingdao Bioengineering Co ltd
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Abstract

本发明公开了一株高裂解性铜绿假单胞菌噬菌体RDP‑PA‑20001及其在禽类致病性铜绿假单胞菌感染中的应用。本发明利用双层平板法从青岛即墨肉鸡养殖场粪便中分离到一株噬菌体，命名为铜绿假单胞菌噬菌体RDP‑PA‑20001，保藏编号为CGMCC No.21410。本发明提供的铜绿假单胞菌噬菌体RDP‑PA‑20001具有较高的温度耐受性和较宽的酸碱耐受范围，有利于工业化的生产制备；该噬菌体对铜绿假单胞菌BP‑20001有强裂解效用，可有效防控铜绿假单胞菌的产生及传播，该噬菌体可作为高效可靠的治疗铜绿假单胞菌引起的禽类动物疾病。

Description

一株高裂解性铜绿假单胞菌噬菌体RDP-PA-20001及其应用
技术领域
本发明涉及生物技术领域，特别是涉及一株高裂解性铜绿假单胞菌噬菌体RDP-PA-20001及其在禽类动物铜绿假单胞菌感染中的应用。
背景技术
铜绿假单胞菌是一种常见的革兰氏阴性杆菌，简称绿脓杆菌，是一种适应性非常强的细菌，除存在于自然环境中，还存在于人体的皮肤、肠道、呼吸道中，主要引起医院内感染，常引起呼吸道、泌尿***和烧伤创面的感染。在化妆品卫生标准中规定不得检出该菌，游泳池水污染铜绿假单胞菌也能引起人上呼吸道感染。人食用了铜绿假单胞菌污染的食物会引起食物中毒，食源性铜绿假单胞菌的污染已成为危害人类健康的危险因素之一。铜绿假单胞菌在禽类体内存在无症状带菌现象，能引起禽类的败血性疾病，具有极高的发病率和死亡率，携带铜绿假单胞菌的禽类肉制品是潜在的危害源，食品中的铜绿假单胞菌一旦大量繁殖，其产生的毒素因子势必会危害到食用人群的健康。
噬菌体是一类以细菌为宿主的病毒，没有细胞结构，必须依赖宿主进行复制和增殖，广泛存在于自然界，专一性、自我裂解能力强，具备裂解细菌和控制细菌生长的功能，可以调节自然界中细菌的消长，用铜绿假单胞菌噬菌体作为生物杀菌剂预防、治疗和控制在禽类养殖过程中的铜绿假单胞菌细菌病，具有低污染、高效率的优势，在一定程度上减少人为活动对自然界的影响。因此利用细菌的天敌噬菌体来解决细菌具有广阔前景的生物防治途径。
本发明在于相应国家全面禁抗的号令，解决细菌耐药性，抗生素滥用的问题，提供一株治疗禽铜绿假单胞菌病的且安全可靠的裂解性铜绿假单胞菌噬菌体。
发明内容
本发明的目的在于提供一种高裂解性铜绿假单胞菌噬菌体RDP-PA-20001，旨在解决养殖场禽类因感染铜绿假单胞菌发病急而治疗不及时，且因致病菌耐药性而导致用药无效等困难。
为实现上述目的，本发明采用如下技术方案：
一株高裂解性铜绿假单胞菌噬菌体RDP-PA-20001，透过电镜观察，该噬菌体头部为廿面体，且立体对称，头部长60.865nm，噬菌体尾部长度为183.254nm,为有尾噬菌体目长尾噬菌体科；基因全长92178bp，不具有毒力基因和溶原基因。该噬菌体于2020年12月17日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：北京市朝阳区北辰西路1号院3号，保藏编号CGMCC No.21410。
进一步地，该噬菌体为烈性噬菌体，其对铜绿假单胞菌具有强裂解性，裂解率达98％，为工业化、规模化、程序化生产噬菌体及治疗铜绿假单胞菌引起的禽类养殖动物细菌病提供了噬菌体来源。
进一步地，噬菌体RDP-PA-20001在pH为7-8范围内中作用1小时，其效价在2.3×107fu/mL之上，适合生长在中性环境中。
进一步地，噬菌体RDP-PA-20001在pH＝12时，效价达到102pfu/mL，在pH＝4时，仍有噬菌体存活，噬菌体的酸碱耐受范围宽。
进一步地，噬菌体RDP-PA-20001的最适温度为37℃，效价达到109pfu/mL，该噬菌体在-80℃的条件下可长期保存，效价维持稳定；在80℃的条件下作用30min，效价维持在106pfu/mL，依然具有生物活性。
进一步地，噬菌体RDP-PA-20001高效感染铜绿假单胞菌，其最佳感染复数为0.1。
本发明还提供了所述的高裂解性铜绿假单胞菌噬菌体RDP-PA-20001在制备抗铜绿假单胞菌制剂中的应用。
本发明的另一个目的是提供一种用于防治铜绿假单胞菌的杀菌组合物，包括有效量的铜绿假单胞菌噬菌体RDP-PA-20001；该杀菌组合物中加入噬菌体RDP-PA-20001后，1小时内有明显的裂解效果。作为优选，还包括用于抗铜绿假单胞菌的抗生素。
本发明提供一种清洁剂或消毒剂，包括所述的铜绿假单胞菌噬菌体RDP-PA-20001。
本发明的有益效果体现在：
(1)本发明从青岛即墨一肉鸡养殖场粪便中筛选得到了一株铜绿假单胞菌BP-20001，并以该致病性铜绿假单胞菌BP-20001为宿主分离得到了噬菌体RDP-PA-20001，该噬菌体RDP-PA-20001对禽类养殖环境中致病性铜绿假单胞菌BP-20001具有强裂解效用，为工业化生产噬菌体用于禽类养殖环境中致病性铜绿假单胞菌BP-20001的防治提供了噬菌体来源；
(2)本发明提供的铜绿假单胞菌噬菌体RDP-PA-20001，当铜绿假单胞菌培养物中加入噬菌体RDP-PA-20001后，1小时内有明显的裂解效果，具有较强的裂解活性，表现出了良好的应用前景；
(3)本发明提供的铜绿假单胞菌噬菌体RDP-PA-20001，在80℃的条件下作用30min，效价维持在106pfu/mL，依然具有生物活性；在pH＝12时，效价可达到102pfu/mL，在pH＝4时，仍有噬菌体存活；表现出了较高的温度耐受性和较宽的酸碱耐受范围，效价较高，安全有效；
(4)本发明提供的铜绿假单胞菌噬菌体RDP-PA-20001在防控禽类铜绿假单胞菌中具有良好的应用前景。
附图说明
本发明上述的以及其他的特征、性质和优势将通过下面结合附图和实施例的描述而变的更加明显：
图1为本发明实施例2中所得噬菌斑示意图。
图2为透射电镜下的铜绿假单胞菌噬菌体的形态RDP-PA-20001形态图；
图3为本发明噬菌体RDP-PA-20001对铜绿假单胞菌BP-20001生长曲线的作用图；
图4为本发明噬菌体RDP-PA-20001酸碱耐受性实验的示意图；
图5为本发明噬菌体RDP-PA-20001热稳定性实验的示意图；
具体实施方式
下面结合附图和实施例对本发明作进一步说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进。这些都属于本发明的保护范围。
本发明使用从青岛即墨肉鸡养殖场粪便中分离得到的铜绿假单胞菌BP-20001，并通过实验确定了BP-20001的致病性和耐药性，以BP-20001为宿主，从环境中分离得到一株铜绿假单胞菌噬菌体RDP-PA-20001，并通过全基因组序列确定了RDP-PA-20001的基因型。
实施例1铜绿假单胞菌BP-20001的分离及鉴定
从具有铜绿假单胞菌细菌性疾病的鸡场的粪便中取样，采用无菌操作的方法，用已灭菌的LB肉汤进行1:10的稀释，取1:10样品稀释液10ml加到90ml普通肉汤培养基中，置37℃培养18-24小时。培养液表面生长有一层薄菌膜，培养液呈黄绿色。
在增菌液的薄菌膜处挑取培养物，划线接种在十六烷三甲基溴化铵琼脂平板上，置于37℃下培养18～24小时，出现灰白色、扁平、表面湿润，且周边琼脂呈现黄绿色的菌落，直径约2～4mm。挑取典型的菌落在十六烷三甲基溴化铵琼脂平板上连续划线纯化三次，然后挑取单菌落接种于5ml的LB肉汤液体培养基中，37℃，200rpm振荡培养8h，得到均匀浑浊的细菌悬浮液。再通过16sRNA分子鉴定，确定为铜绿假单胞菌，将其中一株命名为BP-20001；并通过实验确定了BP-20001的致病性和耐药性。
实施例2铜绿假单胞菌噬菌体RDP-PA-20001的分离鉴定
本发明实验用粪便样品为2020年从青岛即墨一肉鸡养殖场中的粪便池中采集，作为噬菌体分离的样品。
(1)噬菌体RDP-PA-20001的分离：
取粪便10㎎置于10ml LB肉汤中4℃浸泡过夜，10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。取3ml滤液和3ml处于对数生长期的宿主菌培养液，一起加入到20ml高压灭菌处理的LB肉汤中，静置于37℃培养箱中，过夜培养，对可能存在的噬菌体进行增殖。取样品培养液3ml，10000rpm离心5min,取上清液用0.22μm微孔滤膜过滤除菌。假设上述滤液中含有噬菌体。用LB肉汤进行梯度稀释，与处于对数生长期的宿主增殖液制备双层平板，在37摄氏度下培养19-23h。双层平板上出现透明的噬菌斑则证明有噬菌体存在，若没有出现噬菌斑则证明无噬菌体存在，需要重新筛选。
(2)噬菌体的纯化
分离得到的噬菌体不是一种，因此需要进一步的纯化，从有明显单个噬菌斑的双平板中挑取噬菌斑透亮、圆滑、小的噬菌斑，置于生理盐水中，用移液枪经过反复吹打，用0.22μm微孔滤膜过滤除菌，用LB肉汤进行梯度稀释，选择可以得到单个噬菌斑的梯度制备双层平板。以上步骤重复4-5次，直到得到的双平板上的噬菌斑大小、形态和清晰度相同时，噬菌体纯化完成。结果如图1所示，其噬菌斑形态、大小和清晰度一致，为典型的裂解性噬菌体。
(3)噬菌体效价的测定
挑取已纯化完成的噬菌体的单个噬菌斑，加入到LB肉汤中，同时加入处于对数生长期的宿主菌培养液，37℃、170rpm振荡培养6h。取噬菌体增殖液5ml 10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。用LB肉汤做10倍梯度稀释至109倍，取107、108、109倍稀释度的稀释液0.1ml和宿主菌增殖液0.1ml制备双层平板，测定噬菌体的效价。
其中，噬菌体效价(pfu/mL)＝噬菌斑数×稀释倍数×10；经测定该噬菌体的效价可达109Pfu/mL。该噬菌体最适温度为37℃，效价能达到109pfu/mL，该噬菌体在-80℃的条件下可长期保存，效价维持稳定。
实施例3噬菌体的形态观察
运用磷钨酸负染法：将噬菌体增殖液20μL(效价109pfu/mL)滴在石腊片上，将铜网放在上面10min后取下，以吸附噬菌体。在自然室温下干燥2-3min，用2％磷钨酸水液染色，2min后吸干水分，自然干燥5min，用电镜观察噬菌体形态。由电镜照片(图2)可看出，噬菌体RDP-PA-20001头部为廿面体，且立体对称，头部长60.865nm，噬菌体尾部长度为183.254nm，根据噬菌体最新分类与命名，属于有尾噬菌体目长尾噬菌体科。
实施例4噬菌体基因组测序
通过对单株噬菌体的富集培养后，在4℃条件下8000g离心15分钟，加入10％PEG8000和0.5M NaCl静置过夜，再加入等量氯仿混匀，静置分层后5000g下离心10min，去处氯仿层和PEG层后，加入限制性核酸内切酶消化处理，并采用梯度密度氯化铯条件下悬浮噬菌体，后期采用TM缓冲液透析3次，每次30min，一部分送华大基因生物测序公司进行全基因组测序。该噬菌体基因组全长为92178bp，不具有毒力基因和溶原基因。
实施例5最佳感染复数(MOI)实验
将噬菌体增殖液0.5ml(效价109pfu/mL)用LB肉汤进行10倍梯度稀释，按照MOI值为100、10、1、0.1、0.01、0.001，分别取每个梯度0.1ml稀释液和0.1ml的宿主菌增殖液(106fu/mL)加至5ml的LB肉汤中，37℃、200rpm振荡培养8h。将培养液10000rpm离心5min，采用双层平板法测其效价，效价最高的组合为其最佳感染复数。结果如表1所示。
表1
Figure BDA0002871919570000061
由表1可知RDP-PA-20001佳感染复数为0.1。
实施例6一步生长曲线实验
在最佳MOI条件下，将噬菌体和宿主菌混合，37℃培养20min，10000r/min离心5min，弃上清，用适量的LB肉汤洗涤沉淀。加入40ml LB肉汤，37℃、170rpm震荡培养，从0时刻起每10min取一次样，10000r/min离心5min，上清液过滤，测其效价。以时间为横坐标，噬菌体效价对数值为纵坐标，绘制生长曲线，如图3所示。并计算出其平均裂解量；其中：平均裂解量＝裂解末期噬菌体效价/感染初期宿主菌浓度。
结果如图3所示。噬菌体感染宿主菌后的30min内效价没有明显变化，表明其潜伏期约30min，感染后30-50min内噬菌体的效价明显上升，之后趋于稳定，表明噬菌体裂解期约为50min。通过计算，噬菌体平均裂解量约为112，表明噬菌体具有极强的裂解和复制能力。由此表明，当铜绿假单胞菌培养物中加入噬菌体RDP-PA-20001后，1小时内有明显的裂解效果，具有较强的裂解活性。
实施例7酸碱耐受性实验
用稀盐酸和稀NaOH溶液调节生理盐水的酸碱度，配成PH值为4、5、6、7、8、9、10、11、12、13的缓冲液，再将配置好的缓冲溶液将噬菌体稀释成1×108pfu/mL，将稀释液在37℃下，水浴1h，用LB液体培养基10倍比稀释后铺双层平板。37℃倒置培养4-6h后计数。结果如图4所示。
由图4可知，噬菌体RDP-PA-20001在pH＝7-8范围内作用1小时，其效价较高，可在2.3×107fu/mL之上，适合生长在中性环境中。在pH＝12时，效价可达到102pfu/mL，在pH＝4时，仍有噬菌体存活，进而表明该噬菌体的酸碱耐受范围较宽。
实施例8热稳定性实验
将效价为109pfu/mL噬菌体增殖液分装到EP管中，每管中加入噬菌体增殖液1ml，在80℃的烘箱中孵育10min、20min和30min，然后用LB液体培养基10倍比稀释后，取样品和宿主增殖液各0.1ml铺双层平板计数。结果如图5所示。
由图5可知，随着时间的延长，噬菌体的效价下降至106pfu/mL，效价依然很高。表明该噬菌体具有极好的耐高温性。
实施例9裂解率实验和裂解率对比实验
(1)裂解率实验：无菌条件下，分别取1mL样品和1mL宿主菌菌液(1×105CFU/mL)，37℃孵育15分钟，混匀后用生理盐水稀释至10-1-10-3个梯度，每个梯度取100μL涂布于LB琼脂平板上，置于37℃，培养24小时，每个梯度重复两次。同时取1mL生理盐水和1mL宿主菌菌液(1×105CFU/mL)作为空白对照，重复以上步骤。选取30-300个菌落数的平板进行计数。该试验重复3次，取其平均值。噬菌体裂解率＝(1-处理组菌落数/对照组菌落数)×100％。
(2)裂解率对比实验
将RDP-PA-20001与目前已有的铜绿假单胞菌噬菌体YAPa、PaP3和Pse-AE-P3进行裂解率的比对，参照上面的裂解率实验方法分别测定裂解率。结果如表2所示。
表2
噬菌体名称 裂解率(％)
RDP-VP-19003 98
YAPa 79
PaP3 72
Pse-AE-P3 68
由表2可知，RDP-PA-20001的裂解率明显高于其他3噬菌体的裂解率，其对宿主菌的裂解率最高，为98％，可有效抑制铜绿假单胞菌的生长，表明该噬菌体有替代抗生素治疗铜绿假单胞菌引起的疾病的潜力，有广泛的应用前景。
应当指出的是，具体实施方式只是本发明比较由代表性的例子，显然本发明的技术方案不限于上述实施例，还可以有很多变形。本领域的普通技术人员，以本发明所明确公开的或根据文件的书面描述毫无异议的得到的，均应认为是本专利所要保护的范围。
序列表
<110> 瑞科盟（青岛）生物工程有限公司
<120> 一株高裂解性铜绿假单胞菌噬菌体RDP-PA-20001及其应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 92178
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
ttttgtaaag gaggtattat gcctgtttca tgtacacaaa gtgttagcaa tcgttactcc 60
atccttctgc catacgtaat caagagcctc gaagaactca cagaggagct ttggaccctt 120
gaaaatgcag gtcagaatga tgaagtgtac atcaccatta acggatgcgg tggacaggta 180
gagaccgcca acgctataat ccgagcgatc aaggagtgtc aagctccagt gcatgctcgt 240
ctcgaaggtg agtgtgcgtc tgccatgact gctatcgcgc tagcctgtga ctccgttgag 300
atcgaccagt tcacatattt catgatccac gcgccttctt ttggcgttgt atctgctgat 360
ttcaggaata gtctgaggaa tgcagcgttt agctcagcgt ggtgcgataa atgggtagat 420
cacgtctaca gtgacttctt gactgagaag gagctagagc gtgtcaaggc aggggaagag 480
ctttacattg gtggcgaaga gctagaagat aagctcagtg acttctttga aaagaaacgc 540
cttgcaatgg aagaaatttc agagtagtat catcaggccg ggacagtagt cgccggccat 600
tttttcggcc aaaggaggat atacaagatg ttcgaactta tgcaggagtt ggagggtcag 660
gctattgaca acagtaaaga aggcgtgtac cttcagaaga tcagaaagga gtggaaatat 720
gttgaggatt acatccatgc tctggaggaa ctggcgctgt cggttatgcc ttccgagctg 780
ctaaaagaaa ttcaggagga ttgcggagta tgagagagga gttcgaaaac tgtttcccga 840
ttcccggcgg tgtcgagtgg cgtaacaccg actactttcc tgtgcagacc gataacgtcc 900
acgtatacgt ggctcttata gaagcagcac accgctactc gctgatgtgg caggcatgga 960
aagccagccg cgcggctctg agggtggacc tgccgagagt catattcgac gaagaccttc 1020
aagcttatcg agatgagtgc gtcgaagccc tccagcaagc cggaatcgag gtgaagtgaa 1080
tggcgacgcg agcagttcac tacgacgagt gggacggcgg cacagaagcg gacagtgaag 1140
aaccatatga cgtcttctgt ggcaccgatg gcgacttcga ggagaagaac ttctcccgct 1200
accgcaatca tatcacctgc aaacgatgcc tgaagttgct ggacaagaag aaggaggctg 1260
cctaatggac accaacaagc taaaggagga ttactaggta tgaatagtgt atgggtgata 1320
tttggacaac tctgggacga ctacagtgga gagtttgacc tccacatgct tggggtactg 1380
ggcaatacca aatcggagac tatccgcagg gttgtatcgt cctacccggg cttaccagag 1440
ccagacgagg agcttcttga tttcatcgta gcgggcctga ttgtaaacgc agagtgtgaa 1500
gtagacattc cgtgcgggaa gacaggaaaa gacgagtggg ttatgtattc tattcgtaag 1560
aaggaggtgt accaatgagt aacctagtgt gggaacttgt atgggaagaa gattacgagc 1620
ctgacagtag aatgggtctg tggattggga accctccgag tgtagagcag ttagctgaag 1680
tactcctagg tgcggactgg agactgacag aggacaaagt agaggaggcc gctcacagcc 1740
ttctgaagag tggtggttac aaagtgtctc aataccacgc tctgagtctc gatagcttct 1800
ttgcagatac tggactagga aaggaggtag aaattgacct cgaagaactc tgatctttca 1860
actcagatga ttgataaaat cattgcaaaa gagtcggtag tggggcagta tacagtggct 1920
aagcagaagg tgactgtgat cgactatgaa gagttttcac gtctggactt ccagcccccg 1980
gccacttggt ttatcaagga tgcatctggc tactacatct ttgtccacac gtctcagcga 2040
gctaaggccc aagcgtggat agatgaagag tatggcaaag ggcgttacac cgtcaatgcc 2100
agccgagtgc agaagaaaca caacacgggc gaggggagca agccagctta cggcgtagcc 2160
actcgtcgcg gacagaagcg ataggaggaa atatgaaagt agtaaaacgt aaaggtcaca 2220
agttggagtt ggagagcgac gagatcaata tgctggtagg acttcttggt cacctctcgg 2280
cttcggctgc gaccacggac ttgggttggt ttatggaaga cctctaccaa gagcttgagg 2340
ttaaaagtga tccggaggcg gtgaataaat acgagcagga gtttcgggca aaggcctacg 2400
gcagtgatga tccctcggac ttcgttattt taatcaaggg tgcagatgga gagtacaaat 2460
gaaggacgta gcaggaaggg atatcgagat cggcgacgca gtagcattct gcctaggcgg 2520
aactggaatg gttatgctgg taggcgaggt ggttgcaatc aatcctaaga ctatcagtgt 2580
taaagctgaa gcgtgggaca agtggagcgg aaagccatca gtgcaagtgt tccttcgccg 2640
gggcgatgcc gtttgtaaag tagaaaacta aggaggagat atgactgaac cgactgaacc 2700
cactgtaatg cgcatcgacg atgtgatcga ggctctacag actcttcgca ctgtgtatgg 2760
caacttgaag gtagcggaga ttgaacacgg agacccgact tggagctatt acccgcctag 2820
cgttgtctat ttgacagaca ctgacgagta ctacatggaa ttcgcgtgcc ctgaagaggg 2880
gtactacgag gagatcgcgg ttctaattgg aggctagtcg tgaaggtcaa gcatattaaa 2940
gcttacatgg aggccgctga ggccttcgct aagtgcagcg actcgactcg ttccaaggtt 3000
ggtgcagttc tagtaaaacg taaccgtatc atcagttgcg ggtataatgc ccttccagag 3060
cacttaaacg ggccgctgga ggacgaaacg ggagtaacca aggcagaggt gaggcatgcc 3120
gaagaaaacg cactgagggc ccttatacgc agccctgaga gtgcccaagg ggctaccctg 3180
ttcgtcactc actcttgctg taaaaattgt gctatctcgg tggttgaagc tgggatcaaa 3240
gtagtgtact ataagcacga atatcggctc gacgaaggtc ttaggcacct acgggagaat 3300
gatgttcttg tcatcaaata cgaggaggat caatgagtta ttttcaacga ctgtatggac 3360
tcgacaagaa aggcgggttc aaggagtgga ctgtagaggt ggtcggagag gaagacggta 3420
gcttcaatat cgtcgtgaaa tacggcaaag aacatggagc catgacctct caggttacca 3480
acatccgagt tggtaagcag aaacgctctg tagaggagca ggccgtgtct gaggctcagt 3540
ccaaggtgag caagcagttg gacaagggtt atcgtgcaac caaggaagaa ctcaacgatc 3600
tccctgttat ggctatgctc gcaaaaccgc ttgatcccgc taaatacaaa gaggggtatg 3660
gctctcctaa actagacggc ctgcgttgca tggctaagaa agccaatggc gtagttacgc 3720
tagcgtctcg catgggtaaa ccttacaatg taccgcacat catcaaagaa ctcgaagaga 3780
tcatgcaaga gggtgatgtg tgggacggcg aactgtacgt ccacggtgag gtgctggagg 3840
atatcaactc ggcggtcaag cgagaggatg cctacgataa gtggatgaag gctgagaagg 3900
ctcttgacgc ctacgctcta gagaacggag aggaggagta tgatcgtctc ctgaaggaag 3960
tggacagcac gtcctttatt tacggtcttc gtaagcggat ggagttccat gtcttcgata 4020
tcgtaaaaga tgacttgaca tttaaggagc gtctggtaga cttccagttc cagtctgcac 4080
tacacagtga tctggggagt attcgattcg ttgagtacgt attctgctcc actttcgaag 4140
aactcaaacg tcttcacgac gtgtaccgtc agaacggtta tgagggtatc atgtggcgca 4200
gcccagaggg cttgtacgaa tctggtaagc gctccagcgg tctccagaag tacaaagagt 4260
tcttcgatat ggaaatgcag atcgtgaact acgtggtaga caagaatggg aacgctgttc 4320
tggtggtgtt tgaccctgta cctcaagagc tgcttgaagt gacgtacggt gactttgacg 4380
aacgcaagga tcagatcact aattttgaga actaccgggg taagtggcta actgtgcaaa 4440
ttcaaagtcg tttcaaaaag accttgcgtg ctcagttccc aactggtaaa ctaattcgcg 4500
ctggccgggt aatcggcggt gtatttgtcc ctgaagaata aggaggatat aaaatgttca 4560
acgctaagtc tgtcaacgtg accactgcaa tcgacgcggc tgaggagttc cagctcttgg 4620
atacttctgc tatccagatt cgaagcgtca ttcagttcat ggatgctctc acttacaaat 4680
tcaactacac gactgggtgt tttgaaaccc aaactcggga agcgaagcac atcagcaagc 4740
tgagtttcaa cactgctgtg cgtctgcaca acctgtacct cgttcccggc tcgacgatga 4800
cccagcacgg cttctgggcc cctgccggat gcgggtatcg gagtgcaggg ttcatcgatc 4860
tggttgtaag ccctgagctt atggctaagg cgcaggcctg taagattgtg aaaaatgcca 4920
aaatccagaa ggatcgtaaa ggcaacattc acattcagtc gatgatggtc gagttcccca 4980
acgaagacta cgcccgtcgt tacaacttgg gctgaagtat aatagccgag tgatatacag 5040
cccgcccaca aggcgggctt ctttgtctaa ggaggtgtta tggaaacctt agaagctctc 5100
gttttgtcga tccttctagc cttcattggg atgttcctcg acatggaata ctctcgtcta 5160
aaatacggca cggagcctgt agtggagttc caagccagtc ccgatcaaat catagaaagc 5220
aagcgaacac tgtgcgagta cagaaagcct tgcagactgc tggcagaagc ggtctacttc 5280
gaagcccgtg gagagccgga aatcgggcag attgctgtag ctcataccgt catcaacagg 5340
gtgcagcacg ataggtggcc cagcaccgta gctggagtgt tatacttcaa atgtcaatat 5400
tcgtacacct gcgatggtag catgcggcag ggcgtcaagg agcccgacaa gtgggagaca 5460
gcaaaacatg ttgcatacaa cgctctgtat gggtatactg ttgatccaac gggaggagcg 5520
gaccactacc tgaacgagag aaaggtaagg agagccaata atggcaggct ccctcggtgg 5580
gctcggaagt acaagcgtac catcaagata ggcaaccaca cattctacaa aggagattaa 5640
catgaaaact cagctcggta ttgctatcga gatcgcactc tcggcccatg aaggtcagcg 5700
gtatggagag accaactatc cgtatgccta ccatctgaac caagtgcata caatctgcgt 5760
agcacgtaat gctccgaagg atatggaccc gaatcaggcc ttctcggact ttccgtacat 5820
ggacaccctt ctagcggtct gcttcctcca tgacgttctt gaggataccg acctgacaga 5880
ggaggactta gagtctatgg gcgtgatgcc gcatatcgta gaggctctgg tcattcttga 5940
caaaaaccga gctgaaagct atcggaagta tatcgagggt tgccgtaatc atccggttgc 6000
aaaagaggtt aaaatctgcg acactattgc caatcttaca aattctgtga tgagtggcaa 6060
tgcgaaacgg atcaagaagt atagcaacca actgagcatg ctcgaaaggg aagcatcggt 6120
tctggaaaac aaggcacaga agaagacaac ccgttccgac aaattcaaag gctacgtagg 6180
agaacagtat aatgtggaat gaatataaag agcagtacag ctacacccca gccaccaccg 6240
aggagattaa agaagttctt gcaagatggg agttcattaa attgcaggct gagtcggctg 6300
tagaggaagc catcaacaac tattctcgtc cttactcttt cttcgagaag ttgcggggtg 6360
tagacaagtg gtctccgaaa gagttactga agaaagacgc agtagaccac tggcttggtg 6420
ttgaagggca cctgtaccga gagggtaaga ttaatgcaga cacctactgg gtagtagact 6480
tccactatga acgccgagat atgctgtcag agttcaaggc aatggcccat atgaaaccgg 6540
atcgagagca tctgctggga ggggctgcta ttcgactgat ccgcaggatc aacaagattt 6600
atgaatttga aaaggaggca taatgtctgt atttttctct tccgatctcc acttcttcca 6660
taagaatgtg atcgaatact gcaacaggcc ttggactttc gaggagcaaa ccgaggagct 6720
tatttctcgg tggaactccc gggtaggtcg ggtagacgaa gtgtaccatc tgggagactt 6780
cgcatttgca ggaaagaaca agctacagcc tgtgctggat atcatcaagc agttgaatgg 6840
ccgtatcacc ttcctgaagg gaaatcattg cgactctcgt ctgtggaagt tgatcgctga 6900
tagcaaccta tcgcacgtcg aagatatccg agactacaag gaagtgagga tcgacggagt 6960
taagatcgtc atgtgccact atcctttcga gacgtggaat accgctcatc atggcgcttg 7020
gcacctgcat ggtcacactc acggtagcct gccttccaag ggtaagcggc ttgatgtagg 7080
tattgacaat cacccggacc atcaggtatt ctcttacgac gaagtgaggg aatatatgcg 7140
agagcaagag tttgtggttg tagaccacca taaataaaag gaggaaagaa tggtcaatgt 7200
gttgatttcg cgtgacaagg aggatgttaa acctaaagct aacactctgg tggaactgat 7260
ccatgacgac ggagttcctt ttatcgtcct catgacttct gatgttaaac aagacgggac 7320
ttttgaagga gtggctttcg aggacgcccg ctggacggat cagtttgaga tcgctcaatt 7380
ccgagtattc gatggcaaag taacgctgag caataaggag gactaacatg aatgatccgg 7440
tcaaggattt caagaaaact gtaatggttg ttgctgcaca ctacatcact cagtctgggc 7500
tgactcaacg ggaagtggca gagcggtgtg gaacccatca gagccgaatc tccaatctga 7560
tgaagcagca tgttgacaaa ttctctattg agagtatact ggagatcgtt cacaagctcg 7620
gaggttataa gatggtagcc tccatggaac ttgaagagca taacgtactt aacttcaaca 7680
tcttcccgga ggaacaagca tgaagattga taagaattcg tggcatatgc ggctccatcg 7740
tacgttcgag accccggtct attcggctat ctcaagcggt tggcaaattg taaccctctg 7800
ctcttatttc tggcagacag tattcctcgt cgtgtggctg gcgttaacgg ttgtagcgct 7860
cgctgctgtt gtaggctctg tattgtattc tctggctctc ggtccactta ccggatatgt 7920
tctaggtggg actctggctg tcatggggtt tgtatggatt ggaatcatcg ccgtctttta 7980
tggcgtgtgg gttttctacc gattctctcg ctttatgtcg agtaaatcgt ccagcaagga 8040
tgggattctg gtatcgtatc taaaggcaaa gaaggctaaa ttctgcccaa ttgtcgaatt 8100
gaaataagga gaagtaggat ggaaggtaat ctcaacattg caatgtacaa agacctgatc 8160
gctaataagc tggtgactgt taagcacttc aacggtatgt cgattataaa gtatgcccgg 8220
aaggtgttct acgagaacct gtggaatgag catccgctcc ttctggaggc acggggtcat 8280
gtatttgacc agcactccgg tgattgtatt gtacgcccct tcgagaaggt gtttaatctg 8340
ggagagaatg gtgctgggag tttcctccat cctaaattcc gtgtgcggct gattgagaaa 8400
gtgaatggat tcatgttctc tgtaactaag cacaacggct cgctaatctt cagcacgaca 8460
ggatcgctga gtagtgacta tgttgcacta ggtcggaagt atgtgtctga gaacgcagat 8520
gactacattg cagggttcac gtacaacttt gaaatctgct ccccggaaga ccctcacatt 8580
gtggaggaag aagaaggtgc ttatctgatc ggtgttcggg acatcttcac tggtcagcag 8640
cagtctgagc atattctgga tgcatacgca cttggtatgt cggctggaag ctcagttaga 8700
attctccgcc cagatcacat cgagtgtagc tgggcacatg ctaagggcct cctcagctcc 8760
tgtgagaaag agggttatat ggtgcggact ggcatgggga cggtgaagtg caagtccact 8820
cactacctcg gtaagaagtt tatcatgcgg atgggcagca agaaggttaa tgcgatgtac 8880
caagacccgg ctggattcaa gcagacgctt gacgaagagt tctatcctct ggtagacttc 8940
ttggtaaatg aagtagagga gtcctgtttc gtagaaatga ctgatgccca gcggcgtctt 9000
ctgatcgaaa cctacttcga tatgactaga atttaaagga gggaatatgc aggaaaagat 9060
catgtatatc attcgaggac tcccgggctc cgggaagtcc tcatttgccc ggattttata 9120
ccaccagatt tgggagtctg gtgggacggc atccatctgc gaggctgatg actaccagat 9180
ggagtatgga gagtacgtgt ggaaggcaga gcgtgtagta gagtcccata agaagtgcat 9240
ggagaaagcc cgaaactcta tgaaggccgg tcttgacgct gtgatcgtgt ccaatactag 9300
cacccgggag cgagaggtgg aaccgtacat tgacatggct gaagagtttg gctacactgt 9360
gatctctctg attgtagaga atcgtcatgg gaataagtct gtacacgacg tccctgagag 9420
caccatggaa gcaatgcgga agcgcttctc tgtgaaactg tgaggaggat gtatgattag 9480
ttatgctgta ggttgtcttc acgaaatgga aggtgttatg agccgtccgt ggcaagaggc 9540
aaaagtgtgg acgttcccgg gcggcgaagt gggcgtcaat gtggcaggca tcgaaagcca 9600
tgcgttcgta aacgttaaag ctcagattaa gaacagcgat gatatcatgg ctctcctcaa 9660
tgtcactgaa gcgatccgag cagaaatgat taatccacac atgacgcttc tgctaggtta 9720
tgtcccgtat gcccgccaag accgtcggtg caatccgggc gacgccttca gtttgaaagt 9780
gtttgcaaac atgctgaact ctgccgggta cgaccgagtt atcgttgtgg accctcacag 9840
cgacgcaact ctgcacctga ttaacaacgt cgaagttgtt cgtcaatctt cggtcttcgg 9900
cggcctgctg aaccgctcag agtgggagca gtaccaactc gtagcccctg atatcggagc 9960
aacaaagcgg accgaggatt ttgcaaagaa aactggtgca gccactgtaa ttcgttgcaa 10020
caagaagcgc gatcttcaga ccggcaaaat cgaaggcttt gaaattctga atccttcgga 10080
tattgatcct gagcttccgc ttctagtgat cgacgatatc tgtgacggag gtgctacatt 10140
ccgaagcgtg tctaaggccc taagagagta cagtaaagtg ctccctgacc tgttcgtcac 10200
tcatggaatc ttcagtaagg gtatcgactg cttgctggat tccttcggat gcatctacac 10260
ctcggatacg ctccctcaaa aagagcacga agcgctgact gtcattccgg ttgaatactc 10320
caaggcggaa cgcctgtgag cttgagtatc tggattgtag agtataagcg ttcggctcca 10380
gagaagaggg ttgtcaaggt ggaggagttc gacaccaagg aggaactgga cgctcggtac 10440
aagacgatgc acgataagtt ttgggaagac ttccacagca agaagcaacc attgctgcgc 10500
cttccatgca tctaccctgt gctgacgctg ccagtagaag aagtcgaata ttgacaggag 10560
gcccagattg atgtagtctg ggcctattca acacaacagg aggaaataac atgaaaattc 10620
tgccacatct ttccaccgat ggttataaga ttggacacgg ttccatgtac tcggaaggga 10680
ctcagcttgt ctatagtaac ctgacccctc gctccgataa gatttatcga aagacgcatg 10740
ccaccgactt ctacgacggc actctcaagt gggtcggcgg acgggccgct tggatggaga 10800
tcgtagagtc ttgggaaggc ttcttcgcgg ctgaagaaga gaacgccgtt ggccggtatg 10860
cccggttcct gactggctat tttggccggg aaatggaggc tgtggagcag cttcgagccc 10920
tacacaagct cggctatctg cctctggagg tcaagtctct gactgaaggc gtcagtgtac 10980
cgatgggcgt gcctgttctc accatcaaga acaccctgcc ggagttctac tggctagtga 11040
actaccacga aacactgatt tctgctatga cttggaagac cgcgactaac gccactatcg 11100
ccgcagaata cgcagctatg ggccgatact acgcgaatca cactggatgc tatgacgact 11160
tcctgctgtc cgttcagttt cacgacttca gtgcccgggg tatgagtggg attgaagacg 11220
cggcccgaag tggtgttggt cacttgacct cgttcgtagg ctcggacacc ttggcagcgg 11280
ctatctacgc cgaggactac tataacgcga ctggtttggt agcttgctcc gttcctgcga 11340
ctgaacacgc tgtagctact agcaacatcc tccgcattga gaaagaaatg gaggatggag 11400
aaggtaaagg tggccgtgag gaagcagaac gtcgattcct tgttgaactg atgggtaagt 11460
tcccgagtgg tattctgtct tacgtggcag acagcttcga cttctggggc gtgcttactg 11520
acattctgcc atccatcaag gatcagatca tggagcgaga tggcaagctg gttattcgtc 11580
cagactctgg tgatcctgta gaggttatct gcggactggg tgatattgaa gttgtcgagt 11640
cgctcgaaca ctgggatatc atggatgcag taaatggaaa cgatgcattt gttctgaact 11700
ttgaagataa gtacttcaag gttattccca ctgagtggga tcaatacgag tatgtaattg 11760
gctataaact tgaagaagtg agcgaggcgc aggtgaaagg agctatccaa gttctgtggg 11820
aaaccttcgg cggtactgtg aacgaagcag gctataagat gctcgattct catatcggtt 11880
tgatctatgg agattccatt acaactcgcc gagcagctaa aatcttggag cgcttgaagg 11940
agaaaggttt cgcttccctg aacgtagtgt ttggagttgg cagttacact taccagtgca 12000
acacccggga caccttcgga ttcgcagtga aagctaccta cacggaagtg aacggtgaag 12060
gcattgcaat cttcaaagac ccgaaaactg acagcaagaa gaagtcggct aaaggcctcc 12120
tgaaagtgta ccgagacctg caaaatgggc gattccccca gttgaaagat aatgtatctg 12180
aagaggagga ggctactggt tccttgcgga cgttcttcaa agacgggaaa tgggacaccg 12240
aagacctgaa gaacaatgac ttggcatcta tccgttatcg gattaagaac ggagtataag 12300
gaggataaag gtgttagcta aatattactt tacaaccttc tgcgaacaag gtcgcccaga 12360
aaataaaggg cacaaaatgt tcaagttcaa aatctgggag aaggagtatt ggtccccgtt 12420
tgctgccact aactcgatgt acgcagacat tgctcacgga gcgagaggct ctgaagtcag 12480
attcacaagt gttgagaggt tcaaatgatt aaataccgaa agggcgatct tatcgctgcg 12540
ttcaaaaaca aagagttgca cgccattgca catcaggcta attgcttcaa caccatgggc 12600
agcggcattg ctaagcaaat ccgggagcaa ttcccggagg cctacgaggc agaccaagca 12660
acggtgaaag gggaccgagg taaactcggc tccctgacct tcacaatgaa cgagtacgga 12720
tcgatttaca atctgtacgg gcagtacaac tatggccgag aagagggacg agtctacact 12780
cagcttgacg cactgggcca agccctgtac actatgtctc tgttccttga ggcagaggct 12840
gacgctcggg cgattcctac ctcggagttg tgtgtaggtc tgccgaagat tggctgcggt 12900
ttagccggag aggactggag caatgtcgag cctcttatcg aggagacgct cggccagttt 12960
caggtatacg tttacgaact gtaataggag gagatatgat ccggggtgca attaagtata 13020
aggggacttg gttggccccg ggctcggagg ctttccagct ttatcaggat aaaaagctag 13080
acaagttgga taagcatcta gccaagctgg acaaggcatt caagaaacta gaagggaggg 13140
agtgacatgt ctattgatct ggacaactta acagcgtacg aacttctcta tttgcaagct 13200
caaataatcg agaaattgag actcaagttt gaagagtctg gagaaggtga gctagagata 13260
aaagacgtgt accggggaat gctcttaacc atgggcagtg atggatacac tattcccaga 13320
ggatgtgaat gcaaggtggt aagcatagac cacgaagaca acttctatcc ggtgttagtg 13380
gagtacacca accaagaagg ggattatcgt caccactgga ttacatacgg agaactggga 13440
caaatggtga gacgatgaga tattatacag gagtcggaag tagggatacg ccaaacagag 13500
agtggggcat ccttcgagag gtaggccgta aactggcaga acaaggtttt attcttcggt 13560
caggaggagc gaagggagcg gactcagcgt tcgaatcggg ctggctggat tatatatcca 13620
ataacaaggg aggaactacg gcagaaatct atcttccgtc tcccggcttc aaccagtacc 13680
agataacgga gacgctcgga gctaccatcc tgccagagga agagttgccg gctgaatggg 13740
aagaagctaa aggcattgcc agtcaaattc atccggcttg gaactctgtc agaaatgacg 13800
ggaggtccgt tctaagcccg ttcgcaagac gagcccacac tcggaacgtt ttccaagtgc 13860
tgggaatcga tctcaacacg cctagcaagt tcttggtgtg ctggagcaag acagaccaca 13920
acgggattcc agaaggcggg actaggaccg cttggcaact ggcgtcctct agaggtgtgc 13980
cttgcttcaa tctgtaccgc catgcagata gagacagaat catggcgttc ttgtaaaaca 14040
aatagaggta caatcatgta tacagttgag gttccagagg ttccggtagt ctgcgatatc 14100
acagacccaa accgcaggtg catgcaatgg attcgcgctc gaatcatccg acttaagaac 14160
aaccagagta cagggttcga agacatttac gttctagagc ctagccacgt tgatctagag 14220
tgcagatggc gagaagagaa cgaccctgaa gagcacgtta gatgggctat tgcgtggttc 14280
gacgtggata tgtgcgacgg gcagaagttc cgccagttga ttcgatacga tatcaacaac 14340
atgttcaagt ggtatggacc tgctaacctg acatgcgaag gcgtgttcac tatcgagaaa 14400
tctaaggtgt gccctccgta tgttgacatg tgaagagatc gaggagctaa agtctcggat 14460
aggggcgaat gctgatcggt tgattcacaa ggtggagagg gggcttgttc ctcctctaga 14520
taccagcaga gggcgaaagc cctctttctt tttcaacgct gttcagcctg aaatgtacga 14580
agtcatggtc gagctagacg gagactaccc gttgtgggta gacgacgttc tccaaggagt 14640
tgcccggctg gactggtaca gacccgatga aaggtcgatt cccctctctg caaagaagct 14700
cttgacaatt tttgcaagcc ttagtaatat caatgctcac acgataagcc acctcctgcg 14760
ggttgaggct agacaggcta atcggtacta cagagcagcc gaattcgcac accaaaaact 14820
gattgacgga ttctgcaagg atgagctaag atgcatgaaa tatccagaga cattcgtcta 14880
ccctaggcag gatgttcccc agacagatca agaggagggt tagtatgttc aagaaattgg 14940
tagccgttgc gtttacactt gccctgcatt catgcggggt cgctcctgct tatgcagagg 15000
acacttctgg ccgcgatgca gaatactgct cggctgtagt tgcacacttg acaagcatgc 15060
caatggaggc tatgctctct gctctggatg ttcgtcccgg ggatgatcct gttatccgag 15120
tagcaaacac catgtatatc tatacatgca aggtggaggg caatctggtg ctggtcaaag 15180
gagatttcga gaagggattc tatcgagcat tcctcgtcca agaggatgaa tacaactact 15240
acatcagccg ggaggaaatg ctatgatctt cgacaagtat attatcgcgt tagatacgtt 15300
caaggatggt actgaggaat acgccctgtt tagccggggc gacaacgggt ttataacgta 15360
catcaacagc ttcgcaacca aatactcagc tattgaccac gcgttcttct tggagtacaa 15420
tcgctaccca tcagcttatg aggaggccga cctgtacaac tgggttgtga tgtgctgacc 15480
gcaggtcgaa ccaaaaattt agagccctag tggggctctc aaaggaggaa caatgagaac 15540
tgttgcagag ttgataaaga tcggactaga agactacttg gttaacaatc aagaacctta 15600
catgtgttat atcatgagtg atctcgtaga agagcagcag atcacctcca aggagtatcg 15660
atcgttcaag gagtggctac tagaagctca cggcatctcc acttgggagt cagttaacga 15720
ttacctgcgg atcaaccggt tggaggatac cgaggtaaac tggaaaaact tttacgtttg 15780
gtcttatttc gacctgattc gaaaggaggg aaaatgaact ctaatagtat tgcaccacta 15840
atcaaggaag gcatgcgggc attcttcgag aacgaagaag agccttacat gtgctacatt 15900
ctggagagat tggcactgtg cagtgactcc agcgtcaccg tctcagacgt gggacgattt 15960
aaggggtggc tagataagac ctttggaatc gggccgctta attcggtaat gcacctcttg 16020
gacgaagtag ctgattcatg cgtgttatcc atgttcgaga ataaccctcc gaacaaggac 16080
tgggaagact attgggaagg ctgggccata ggcagagcgt ggtgcaactt ctacgtgtgg 16140
gcctacttcg acttgattcg aaaaggaggg gagtaatgga gataatccca atgctattcg 16200
tcctcgcgct cgcgtgggga attgtgttca tgtggaccta catgggtatc acggagtatt 16260
tcaaagataa cttcccagct agtgcgacta aagatcacag agagggtatc gaggcttttg 16320
ccattgtact gggactgcta tggcctatta cattcacagt gtatgctagc tttctgctac 16380
tctacgcaac atacagcgtt ttgaaagtgg tgttgttacc taagtgggct ctgtctatct 16440
ggaacagccg taaactactt aagaggatcg caccatggaa caagtagggc ttctcattct 16500
agtatgcata gtcgtcggag tggtctgcct attgttctac tctgtgatag ggatgttcgt 16560
atgggaatct ctacgacccc ttcttgggtg gaggggagaa agtacagaga ggcgtaagag 16620
agaggcccgg gcggtgttgg ctggaatatt ctggcctatc gcagtaccgt ttatggctat 16680
gctattcctc gttagtctcc ctagaaaagt gtggagactg actagggtag ctatcacagg 16740
agaggaggac tgaatatgtt taagaagctg ttcgcatggt tccgagagga agaaagtaac 16800
gatgagccta aagtgttgga gtctcgggct gaggagtttc aggtagagga gtgctatgat 16860
ccagcctccc ggcctgtaaa gtatataaac gctaagcaga ttaaacgtct gaaggaactg 16920
gatgctaagg tcggagaccc ggttaaggct atttgtgcta cattcttcga ctggaagcga 16980
tggatcgtag accgcaactg gagattttcg atgatggccc accgtatcga tgagaaggtg 17040
catattacag attctaagac cggaacagtg ctacagtgga gagctaccgc agactcagac 17100
ctgagaccgg taggattcct atggagagat atccccttgg tcagggacgc cgctgacctc 17160
gtactgacca atatgccgtg gatgacttcg gaggaaaaag aatacgctgc ccttatggct 17220
aggaaggcta tcgccgcttt ggcagataag aaggactaca tcgacaagct ggtagcccgc 17280
aagcgtagcc gggataacta caaggctggt ctgattgacc gtgcgttgat gatggagaag 17340
tatgtaggag tccaagaagg cgatgagtgg gatatcattc taaaagaggc tagagagcgt 17400
ctggaacggt ctatccccac agagagcttg acaatggact gggaacgttt tacactccca 17460
taaaacacag acaggaggaa tacaattgaa agagcgtttg gttaaatttt acactatcgt 17520
gaagaccccc gaagagaaca ttgcagtaca acagcacctt aaggcttatg gtatcgagtt 17580
tgggagcgga aacggcttca tgtacgacaa ggctcaagtg ctactgtggg ttggagataa 17640
tctgctggac tacactcgta aaagcgacgg aagtccaatg acattcctgt ggtacgagga 17700
agaactcaaa gaggccattg gatactcgaa atcgacctac gacttccgaa gggtagactt 17760
ccatttcgtc actgagaaga aagtgaccgc gtacacgctt catgagtgtg acactggtcc 17820
tactacagag ctaaatggca cctacacgct tcaagcgttg aaggaaatca ttgcagaaat 17880
ggaggaagag aatgaaaact aaagagatcg atgtcagcaa tttcactgcc gaacagtttg 17940
atgcgttcct tgagtactgt cagtacgctc aattgaaggt aggagagggc gtcgttgata 18000
agatcaggga gtggatggaa caccctatct ccgtagatgg gccagatgat agtccccggt 18060
gcctatacat ccgggatcat cccgaagacc ggttcattag ctacggaact ggcctgcgaa 18120
gcaagtggca acccgatgtg tatgacttgt atcggcctac cttccagact aagtgtgtga 18180
tgactctagg gaaggcagac aagtatgtgg tcgtaggaga cgagaccatt tcactggagg 18240
agctaaagac tatcctagct aagcaagggt gtttgctgcg tttggagggt ctaatgtctg 18300
agcaatattg gtatccagcc cggatcgggt gcataaagag tctgaaagca gtggcttggg 18360
aggcttggtc cagagacttc tgggatgatg agaatttgaa caagattctc cgtcacaacg 18420
ggttcagtcc tgaccagagc aaagaacgag cgttgcagta catggaccag attaacgaag 18480
agtactgcaa ggttatgcag ggatgcttgg aagcagagga catggaatac aatcagtaga 18540
gacaaaaagc cccgggcagc aaaagctgcc cggggctctc ttttacacag ttacggtcta 18600
gctgttgtca gcttattccc aataccaatc ctatagattt gcattggcca ttctccctgt 18660
gcaagcggag cttgctattg tatgcacgac cccaatcccc tcccagaaat aatgaccact 18720
tgaagagtgc gtttactcta tggctgggaa aataatgatg tagtgtggcc ctacgtccga 18780
gtgtatcctg ttcgggagtt gtggttctat ttccgagcta cttaaccacg aacccacctc 18840
gctaccctaa cccttagtaa attcaaaata atgggtggcc gtggtctttc cttcttagcg 18900
acagctaagc tatgcaaaac taatgccaac gggctcccgc cctgccgggt gaaatctttc 18960
cacatgttgg catggaatat gcatatacgt ttgatagctt cgctatcgga ttactctggc 19020
ggagttctcg cactctcaga gggttgacca tcgacccatc aaatcattgc aaaagggttg 19080
tctcttttcc ctgtgcaata tccttgccaa gtttgtccac tctttcaagc aacgcttgaa 19140
caacgtttac actgtgaaca tcctccatct gatagagatc gatacattca tggatggctg 19200
ctatcttggc atgttgccat gccgcatggg cttgcatagg atcgttccac gatccaaggg 19260
tagactgctt gcctagtgtc ctacactgag caacgtatac aggcatcccg tacttgtcat 19320
agcgtgctag gcggacgcct agagggatat agctatccca cgtattggca cggttattgc 19380
ttgtcttgtc gcagttggag aatagagtgt gcatgtatgt aggcgtgaac tcgcatgtat 19440
gcggagcata gagcttgttt cccgggtaga ggatgttctt gtctaggcgg tagcctacgg 19500
cgtaccctga cacattcacc acccaatcat agaacgcttc aaagcctgtc ttgggcttgg 19560
cccattcctt gcatatctca tcgcttgcga tggcacgttt acgcatggcg tagtagattt 19620
gcttagcgta atggatgtga atcttggcat gattgttgct tgtcattgtg cctccttgtg 19680
tgaatgttgg ttactcgata gcggcagcta tcaacctttt agtttaacgt atttactggg 19740
ctggagtcaa tgttttcagt gtcaccactt ccggtcgcaa atataataga ggtagagcct 19800
acacttcaca gaccactttg aaagctccgc tttcgagggg ttacccttgt catgcttatg 19860
caattcgtgt gccatgcaaa acgggatagc ggagctatca cagggtatac tatagcatgc 19920
acccgcatgc aaagtgaagt cacttttgaa gcgcatgatg aactgtatgc aatttccatt 19980
gcaaaatggg aacgggtgta agctgtcgct taacatcacc gggacaatgg tagcgcggaa 20040
aggggttata cccaaaatct actcaaaaca accaacatcc tatgctaatc gcatatggcc 20100
ttaaaacctc ctacaagcct cttactgtaa ggctttcgcc ttgatggtgc agactaacat 20160
agcgaagcta tcgcttcata tacggcctta caggccgctt aaaaccgata ggttttaggt 20220
gggttattgt ggggttattt gatagggata tctgtacagc tttcgctgtc atgctgttat 20280
acatgtaacg ttaacgttac aagtggtcta tcttgtcatg ctggtgttac ctctacagag 20340
tgttacaaca ttacatgtta attacacttg aaagctccgc tttcgcagat agaacaaagc 20400
ccgcacaagg cgggctgtta gagctttgtt ggttttagtg ggcttagtag ttgtttttga 20460
ttaggcgttt cactgccttt gcgatcacca ccacgccggt atgcctagga caatgcctac 20520
tgcgagaaat ggtaacgaga taggccatat tgcacctagg acgtagataa catctttctc 20580
tttgcgggta ggccattcaa attcagagct agcctctttt agcattagta gcattccaga 20640
gactaaagcg cctatcacgc aatagaccat tgctatgacg aaaaagagga tcagatagtt 20700
aaccattgtt cacttgcctc ctatgagaga aagcccggct gtgccgggct gggaatgggg 20760
ttagctgtga tggctgcgga tgcaccgagc ttcatcgtgg agattgcgga ttcgctcggc 20820
ccaaggatgg ccagtgaact ggtatccacg atgttccggc agcagcatca ggcggagggt 20880
tgcacagcgt tcgttctgta ggcgtgccag agtggcgttc atgatctctc ctcgattagg 20940
tctcagtgaa tggttagcga cgaagggttg cgagattgga cccacaatga ccgcaccact 21000
tcttgcctaa tgcgataacc caattgtgtt tgctgttacc gccgttgatg gctccacact 21060
tcgaacaaca gatttgaacg tttttcatgg tctttcctat gagaggcccc ggtcacccgg 21120
ggcttgttac ggattgatta cttgcggtat ttcttaatca agtcgtgggc catggcgata 21180
gcatcagagt aggtttcgcc gccagcttgc atttccttga cagccttatt gcgaagcgag 21240
ttcagcttgt ttccgttgat gctgcctttt tcatccttgg cagcttcaca agcatcgcgc 21300
agagttttca gggcttggta ggttgcttgc atcgtctttt acctattcag gtgctcaggg 21360
tgattccctt tgcttgatgg ccattctaca gcaatggcct aagctgtcaa caactttatt 21420
tgagttcctg ttcagggtaa acggagcccc ttcccgtcgg agaataccac cagattcccg 21480
aatccgatgt atgtgctgga gcttccagct tcgtgagcct tcaggcaggc atcgactata 21540
gtcatgccat ccccgggttg aacttgagtc catttggttt tagcccagcc gtaccgcccg 21600
tagtaccctt ctctgacaaa ccaaaccccg tcttccctgc gggcgtagtc gtagccgtca 21660
ttgccaatgc gggagaactt cagatttgcc tttgccattt tgctagtcct tctagcttag 21720
gagacgcctt gtgcgtctcg ataggcacag tatagggaac cctctagcct cacgtcaaca 21780
ggtattttcc ggattattct agtttttcta tagccactag tcgagcccgt agagcccgct 21840
tagtcgctcg gctcagcttc catttggcgg ggtttcgcat atccttttcc cgctgcttgg 21900
cgtccttgta ggccttaata caatctttgt aaggacggcc atagccagca caggcaaaga 21960
gcgctgccag cttcacaact cgactgtcgt ggtggaccca tcgtgccgcc aaggaaagga 22020
ctggccgtct gccgtcgaaa tggcagtatt ccgcaatgat gttatcctta tacttgttat 22080
tcatttgatg atttctcctt tgtagttgaa tcggcagagg atcgactcgc agccctcctc 22140
ttcgtcgaag aaggatagac ggataccagt atctgcggga ttgcagagga gtgatacaat 22200
ctcctgagct tgaccgaaac tccgcacaat ttgagattcc acaaacacgc cggttatggt 22260
gacaaaccta actcggcaat cgtaggtttc gaatatggtt cgaagcttca tcctagctcc 22320
ctcagttaat gcaagatttg cgttttcaga ttggggcgcg ggtcgatatc aatggcattc 22380
tcctttgagg cgaaaaggat agaccaatcc gacaggtagt cttttgctgt catatctttt 22440
tgacgtatat gctgccatgc ggtcatgtaa ctatactctt cgatctcgtt cacgggagtg 22500
gtatatgccg ggctttcaac atcagtaaac acgatccgag ggttcgccag ataaagatca 22560
ggtgactcgc attgaatgtg aaacactcgg caatcttcgt ctaaggtgac gattgcaagt 22620
cgatgattgg gtgctaccgg gctcaatccc gaatcgtatt cgtcatgaac cgcgtagtcc 22680
cggatatgct ttcgaatact ctctacagct tccataagga aactacggac agtttgaacg 22740
ctgtaggcgg aatccaaacg gataatcagg gtattcatgc ttttatctcc tattgtgagc 22800
cccggcttgc cggggctgtg ataatcagtt ggttacggtt tccatcctga ccgggcgatg 22860
tattttatca tcttgtgaag tagcttcttt cgagaggctt tcagtgacgg cggcacttga 22920
gtggtaactt tcttgctgcc gaggatagag atcatgccgt cgattgcttt gcactggtaa 22980
gagtcggtat attggcgttt cattgtcagt actcctagta ggattttctt caggctgcca 23040
gttcgagcat tgccacatca tgggcgtctt tgaacaggat agcggcttca gaggcactat 23100
acccgcgctc caccatgaat gcaacccact tttcactgct acgatcatcg cgggcttgga 23160
tggtctggcc cttgcgcagg tgagcacaat ggttaacctt gatctcggcg atgatgtact 23220
cttggatttt gatctggctc atcggggcgg tcctgatgtt gtttgctggt atgggtgcat 23280
tatggggccc ttttcgggcc ccgtcaagca ttagataccc aattccgcgt atttttccat 23340
cttacgttct gcgggagcca taatcatggc catggtggca ttttcggtcc agtcggcgat 23400
cacttcgcca gcgctgttac cgtagattgc ccacatagag ccgacgatgt tgtccgggcc 23460
gtcacccttg cggaagcgga tgatatcctc atcggtcgat tgcaggctgt cagagatttc 23520
cttgatatcg gtagacttct tcactaccca ctcgccatcg ctgccatcct tcacgctcac 23580
ggtgaagcca gtacggattg cacgcattac tgctgccatc actacagcct tttcagcccg 23640
catgcgcttt tggaccagct cacgtgcctt gatatcctcg acgagtgctg cacaatcgta 23700
ctggcggaca cccggcttgc ggtaggcgtt acgcagagcg atgcggaaca gggtggcgta 23760
gggcaggcta gtgccagagg ccttgtccat gcgggcgacg tagtgggcgt atgcgaaagt 23820
gttcattggc ttgttcatgg ggagtctccg gtttgtggtt tgtcttgctt gagggccatt 23880
ctacgcgaat ggcccaagct gtcaacccta caaaccgaag attttcaaaa tttctttcga 23940
agcctcaccg gccgagtcat cccctaggtc tagggacaat tgctcggtag gacgtggagc 24000
tacagtttgt gtgaccgagg cggacaatag tccggcgcgt accatgctag cccattggtc 24060
tatgctcacc tcttggacgc aatcagcttc cgccttgcgg aatacacatt gccggatgcc 24120
gactcttaca tgttgcttgt ctacctcata gactctgatc cgtgcggtag aatagaagga 24180
cagcacatcg tattcatcgc caaaccaatc gtctagctgc ctaggggacg tgaaggcgaa 24240
atactcaaaa tggcgaacat ctggtatccc gtcatgctca gggacaggcc gctctggagt 24300
ggaagacgaa ttcgtcatgc acccgttaac ataagggccc tcgccgttct tgcactcgat 24360
tcgataaact acagttttgt cacccatgac cgtccctcta gatcacatta tttgctggct 24420
gttctacgct tgtaatgcat tctactgccc ttttcgggct aagtcaaaga ttacgaagaa 24480
ccaaaattgc acccacattg cgtaggatga gcgcccgtat tcggacattt cctgattgtt 24540
cattttgtat gcggctgcta ggccgctttc ctcgaaatag tcatcaacgc cggcgaattc 24600
gcatccctct ggggttgatg agataccggc agtcatcatg accgagttaa ctgccatttc 24660
aacgggcgag gggaacaaat gaatctcccg gagacggatg cacatatacc acggcattcc 24720
gtactcaatt gcatagttca ggcctttttg gaaaatttca gaaagtttca tagatacgtc 24780
tcctattcgg ggattttgtt gtgaacagtg aaccgattca tatctgcctt gtgccgggtc 24840
tcgataacag gcgtaaaagg cgggaaaaca tcattcgcct tgccgtattt cactatctgc 24900
tcagcatact tctttgccag cgcatgggta gtgaacagag tcggctcaaa ctctggtcca 24960
atgctaccag tgacgatgta gcagtgattc gggctttcat tacctgcggt tcgatcaacg 25020
atggccattt tctgtatcct tttgaatggg gcgcttatgc accccttggc taggcattct 25080
acgctagttg gctagactgt caagcgttat ctttgaaatg ttcgagaatt gacagcggga 25140
gctgtgaatg gtcgatctcg aaagcttcgt atgtgtctat gtcactggac tgcgccaccg 25200
actccgctat cgccaccagt ttgtcaggta gttggtggga ttggaacata gaattacctg 25260
agactccagc tacgatgaaa gggatggccc atacactcac ctccgcctca tacacttcta 25320
caatccatga tggatcaaag ttatcctctg gaaaacagct agcttcatta ctggcgttag 25380
ggcacgaata atcatcttct acgcggtcct gaaactcttc ggggatatcg acaaccagag 25440
cgtagatttt accgccttcg gccatcatag cttgctgtaa ttgagcctgt tctaggcaac 25500
gaacgatgca ttccgcttta gcatactctt cttcatcgtg ccattccata accttgttca 25560
cgttgtagaa gtatgacatt ccgtcatgat ccgaaacgtc ccaaggtgcg gccaatcctt 25620
ccttgccttg acggttcatg atagctttca ggccttttgc agttgtccca tgaactaaca 25680
gcattttatc gatctccggg ttccagattg agcctttcgg ctgttttcct ttcttgaggc 25740
ctattctaca ggtacaaaaa cgcccgtcaa gcattatttc agcctaacgg gcgatatttt 25800
acagcattgc cagcttggga agagcatcga cagtggtttc ccagcaagct acaggacgcc 25860
acgtggtttc gtcatattcg agcatgatgg ctcggtcggt ttcctgatga atccactcct 25920
ctagcaggcc gtcattgtag gtattccagt tgatccaacc gagagccttc aaccgatgtt 25980
ctaccggaaa agctgcaaac tcagctttca gcttggacat ttgctcttct gcgtgccgca 26040
gataggcgaa acgctcggtt atgttttcac tgcactcacc gacgaagaac gtaactgtgc 26100
gtagccagcg gctctcatag tatttcactc ggcgaatgat gatggcggcg cggcgctgta 26160
gttgtttatt ggtgatatgg ggagcgatca tggcagttca tcctctgcga tcagttgaca 26220
ggtgaaatcg taatcgaaaa caaagcgcgg gtcaacctga atattgaatc ggctaaggta 26280
tcgggtcagg cgggtatcgg cattagtcgc cacccaagca tcgccgaact catcggcctc 26340
ttccactagc cgagcattac actctgcggc ttgtcccggg agcgagtgat cgataatgta 26400
atcatcacat tgaacggcag agcaaagggt cagagttaag gctaagatcg acattggagc 26460
attcctgttt tgatggagat tgcagattac agtagttcgt accgatagtc aagcttcatt 26520
tggcggcaca gtttgttcca tttcaccagc aggcggagtg cagcaggcat cggcagagcc 26580
tcgatcaaat cgggatcacc accgcagagg cccatagatt cgctgaagtg ggcatcggtg 26640
aatagtgtga tctcaccgtc ccgctttccg ccgacaaccc ggacgccttt gctccagtta 26700
gcttgagtca ttgcccttgt tcctctagtt gttcgattac agtttcccac cacaagcgcc 26760
gcatttgata ggccctatcc gatacaaaga aagtcatttc atcctctggc tcatcatcgt 26820
atacggctct ttcgtaaccc ggtatctctt tcttgagctt agcgtgcaag gtaacgaagg 26880
gccctagtag ctgatggata aattcttgta cctcggtagc tttatttctg gacacattat 26940
cgaacgccag agcgcacatg aacgaattat tcttgtaaaa cttcaggtta tttttcagaa 27000
tagcggagat tttcattctg cggccctttt ttggtcagat agtagactta gcggcatcgc 27060
ttgcggattc tttatcctcg aacggacccc aatggaaggc aataggctcg ctaaggctag 27120
ttccgaaaac gctcacgatg tagcagccat aaacatcctc atagacagta gcagtacggc 27180
tatcaccgct tgcgtagtct tcacttttga actgagctac agctttcatt ttgagtctcc 27240
taatgggcgg tttgtcttgc ttgatggcca ttctacgcga atggcctagg ctgtcaagca 27300
ttattttctg tcgtctttca gtgtgtaggg ccagcccagc gcttgccaag cccggaattt 27360
ggctaccgtt ttaagagcgt gcgtttgatt gatacagaat cctagcacgg tcacccggtc 27420
acctctgtaa ccgggctcag tacggatgat ggcccacctc tggccccgtt tctctgtcga 27480
gtaagaggcc gtcactggta acgctcgata tcacgcagag tcttaagagc atcgttgtgc 27540
cctatgctca ggttaggatg atacccgtac tcactcagag cgttgattgc agcccggtta 27600
atgcccttcc attccaccca tgctgcaatg acattcccag tcacccggac actctcagca 27660
ccatatgcaa cgaatcggga gttatggtag ctaacccgca gaatgttatg cccggctttg 27720
tttgcaatgt agcaggtaga gccgtggcca ttcattagac caccacggcc agtagaagct 27780
agctctaagg ctaggtcgat cttaggcccc aaggccttaa gctgggattt gttaagctgc 27840
cgcatcactc gcctcctttg tagcaggccg cgcccgccgc tgtctgatac tgagagggga 27900
accacttcac tccccatgcg gttagctgag cttgtagctg ccttgtcaga ttctgccctt 27960
ggctagcctt gcgagtttcc ttgtaatgaa tttccccgtc cgcttgcacc atgaccccgg 28020
acattccctg ccctagggca tcgtatatga cgattctatg cttgttcttg tgatcctgat 28080
acaagactac cttgcatccg tttggagccg ctaggctccg cacggttcgt tctaggccca 28140
tcatcttaca ctgattgaat ggtgcgtaaa cagattcgtc cattgtgtta acccttagca 28200
atgagatcga cagttttctt tgctgtgccg tgggcacgga agccgataat atactcccgg 28260
tcggccactt ggcacaagcc gcacgactta caattcacct tgtcgctttt ctcagccgga 28320
caagctacca ctttacgtcc tttcggcgta aagcttacat tctccgcgtc catcgggaga 28380
atagtcacta caggtgcgat gttcaggtcg gccagttcat cggcgtgcgc tacattgttg 28440
cccgacaggt tgatagtgaa gccgctacga ttggccgaca atacgtgctg gcggttcatg 28500
aaattgttca tcgggtaatg tgtgtaggtg aagccgcgac gtcctttgtt ggctttcacc 28560
aaatccatta gcgcaacgcc gtcgataacg tcatcctgac ctaccaaatc accagcttga 28620
ttatgacgcc acaagtcgcc acgggagata gatcgcacct gttgaaggaa ctctttccac 28680
tctacgccac gttcaccctt gcttagcttt ttccagtgaa tagcagtagg cccataagcg 28740
gcatagcagc ctttagcctt gagagggcag gcatccgggc aggtgccgtt atgagacgtg 28800
ctaacaggca tgggaccggt tttgctgttt ttggaaacga ggttaagaac gaagtgcatg 28860
acggttatcc tttatctagc taggcggcct gtccgccttt cttgctgacc attctacagg 28920
aggcgggaac cttgtcaact gtttttcaaa acaaaccaca caaaaatcga gaaagacagg 28980
atggtccaga acatccaagg ttttacactg cttaacagga tggctctacg gtgaacctct 29040
tccggtacgc tgtacccttg gacattggta ggctcatcct ctggcagacc gtaggcccgg 29100
gcacgttgct tcattatctt ccggcgtcgg gcttttagta ccttagccat ttcgttgtat 29160
tcgtccgggt ccgttgcatc cctgacccgt cgcaattgtc cctctagctt atcgacttcg 29220
ccaagcttgc taaggtaggg gtctatcctg tccctcagcg gcgtttcatc ctcggtctgg 29280
tcttgaaccc acgacgggag ccgctgtcgt ctagccataa cataccgccc attgtcccga 29340
agtcttaaag agttttatgt ccttttgagc gggcagaccg gcgaactccg gcatggcgaa 29400
caaccatgca aggcaaaaag catggcgagc atcgggacag ggtacgcatt gacgaaccat 29460
ttttggtatc tccgctgggg ttgagaatcc taaaaccact cgacgaatgg gcttaggatt 29520
gccggcgaat cggcccaccg gctaggcaca aaccttacat atcgtcgcgg aagtcgaaag 29580
ctcggcctcg gctagctttg cgattgtcac gcgatttgcg ggactcttta cgttcggaac 29640
gtttggcggc ctcgaactgg taagcggaat cgaagtcgga agccttgata gaacgtgcca 29700
ttttaaatct cctttctgtt taagtttgtc ttgcttgagg gccattctac aggaatggcc 29760
taggctgtca agcttttaat cgaatttata ttcgattccg tttaccttgg cgcgggtcag 29820
agtctcaagg ttgatagtgc ggaaagcgga tttaccctct tccatgttca gcacctcggc 29880
ggcggtatac aggtgcggct tgccagcgct ggtgggcttg cgggtagcaa actggccgtt 29940
agcatacgcc gacttgatct gacgtttaca cgtcattttc cgaacggtgc cgtccgcctt 30000
gataaactcc acactgaaga aactgccatc cttcgcttcg tcgagaatgg accgcttgcg 30060
gtctacgcta acagacttag ccattactta gcctcctttt tgttcagctt gacagcccag 30120
cgcttttcag cttgagcgcc gttgtctacc actttacggc cattggtggc cattgcacgg 30180
gcttgggtac gggttttgaa aaacatttgc atctgctttc tcctcgccta tcggcgtctt 30240
cgttgtctgt gggtgctatt ttgcagctag tgggtcaggt tgtcaagcgt ttatttcact 30300
aatgagcaca tagaattcga tcccgcgcca agcccctccc accttggcca tatcaccttg 30360
tatcttactc actcggccag tccatatttc tcccgtgccg gcatgcttga ggaacttacc 30420
acctaccaat tgttcgggcg tgtattggca cgttccgtcg agctttttcc gaatgtcacc 30480
ccggatagcc acgatatctc ctactctgac ggtcatcagt tctcccctca tgccgttttt 30540
acttcgatag accgagcgac aatcggtccg tcctgcccga tattgtacga taccaccggg 30600
agcgattccc atcgacggag catccattct gttttatcgc agtcgtctgc gaaaggcggc 30660
aggccgtcgc ggaaagcgat ctcatccgca ataagattaa gtatatcgtc caacccctcg 30720
tctaggtcga gcgttcgacc aagcaaggcg aaggcttgaa tcaaggcctt gtaactcagt 30780
tccgacagaa cgattttaac agaaaggtta tccatcactc ggcctcctct acagtgatat 30840
cggatgggta tccatcatga tttaccagag acttccgcac ctcctcggta tcacacataa 30900
catcgaagaa aacaatatcg atcaactgtc cgttctggta tacgttccaa gcttgcattt 30960
ctgaatctcc tttatgctgt gcggaaagag cgtgtgtccg acttcacgtc aaagataacc 31020
ccgacgcctt ggacggttcc attgcagtaa aggctgtgcc aacgggctgt acccttcgaa 31080
tgcttcacca gataggtgcg gcgacgatac atgtatgcgc cgtcttccca ttcatcatct 31140
ttctcgatag ggaagtagcg gttcaaatct accgcaatac gatcaagctc gctacgattg 31200
taagccgcgt agagtgcccg atgattacaa agagctttca tatctgcttc ctgcgtgggt 31260
gctcagggtg tttccctttg cttgagggcc attctacgcg aatggcccaa gctgtcaaca 31320
tctttttctc aattattttc gttcttcacc caaactagcc tgcccagcgt ttcaggacgc 31380
ccacgtacct gttttctcgc catgctagtc cgtctgaaac cgtaatggtt gagtagttca 31440
agctggccgc ctccgtgatg gatggccagt cgccacggat agacgactac cttttcctta 31500
gtgctggcgt attggttcgg tttggccccg atcaattcgc accactcttt ccataccgcg 31560
ccgtggccct gccctcgtcc agctttggcg tgggctatct cgtggcgaat ggtatccaaa 31620
acagcaggca agccgttgtt agctacataa tcagagccta gctctatgag ctttttagta 31680
taatggcatc gacctaatgt agccttcagc ttactattga atctgaattt ccagccttgt 31740
ttgaggagac cccatttgtc catttcctgt agggccaatt cccgggcgtc attgtgcaac 31800
attgtagagt accgttccgt tgaatgttgg ctcattgtgc ctatatagag acagcctgtc 31860
aacctatttt atgtagattt ttctctatat tttcgcttgc cttcttttct tgcttgtgta 31920
taatgacagg caggcgggca acgcaggcag gaggccgagc accggaggca aacacggagg 31980
gagcctgaga tttaggttgg cacgctaatt gcaattgcaa agactgtgcc ataaaaatta 32040
tgttgacaaa ggggtttgtc atagtagaat gggcacatct taaacgcggg gtgcgagtcc 32100
ccaactacat atcgccttca cgacggatag tcgaggccta cgcctgcccg ggaaactggt 32160
gcgggcttgc aacgttcgtg ccaactgagg tccgtcctag gaatttttag ttagtgtgct 32220
aacgattaaa ggtagcttgc taaccaatta gatagcctgc taacgatcca cccttctgag 32280
aaatccggct aaatttcagg ggaaattcct ctgtagaaaa atccccttct gtgaaaaact 32340
gtccagttcc taaaaatctg cccagttctc aattttgcag tctgtgaaaa tctatccagt 32400
ccctaaaaat ccatccggtc cctagcttag atcgcctcgt attcctcccg tttaattctg 32460
tgcagtactc ttcgctgata ccagctaatt aagtatgcta tacaagccct cttctcgatc 32520
tctgtggcat ccatctgaga aacaaactct ttgatatccg ccagccaata cgcatattcg 32580
ccaccgttgt caatcttcat tctaaccctc ctattaagcg tcacactgtc cttctacggc 32640
taagtagtag ctcaccctgc ccacactcga acggctctta tacgagcatt ctgagccttt 32700
tcctgagaaa tttcatattg cccatattgg ccgaaactcc cagaaatcta aaaataattt 32760
gaaaataacc cctgccacga aaaagcccgg ctctcgccgg gcaattctaa aataatggtg 32820
gcttattttc gccactctgc tccgaccacg gtccattcgc gggtccaccg cacctccgaa 32880
gagctgtgca gcttatccca caggggatca tccacactaa tacacttaac taccctatcc 32940
ggtacagagt aataggaaga tgggcggtcg tctttctcgt tgtactccca ttttacacca 33000
ttcactaggt cgaggttttg aagttcatcc ggtacatttc cagattgaat gtcggttgcc 33060
tttggagaca ggataaaatg gatactccac cgctttccta ccggcttagc gaacacacta 33120
acccacttcg aatctttctc agtgaattct tttctatcta gttccctctc tagatggtgg 33180
taaccccacc gacccccacc ccaccctcgg ctagagtacc ggcccttttt agctaagagg 33240
tgccaagtga tcttcacctg tccgaggtag acagcctcta gtttctcctc tgtcccgtag 33300
ctaagaagag tgcatcgatc ccctgcaacc atatctttga cggttttggc cgcctctttt 33360
cgctctacga aacttacagc ttctttgtag agagaggagc cttccggcag tagaacgtgc 33420
tgagagccca tccgaaccca cacgcaaggc tcttgaataa ctcccttgat tactgtgcag 33480
ttgtagagca aggtcgagat attcccggta ggaacctcta gaacaaagcc cctagggtcc 33540
gtcacacgga aatatttgtt ctcagtgacc cagcgttcaa cactgtcccc gatgaaatgc 33600
cccgagacag ggatgttgtc tatgaagcaa gtctcggcat tcttacctgc ccagctcata 33660
ccagtctgct gctttttctt gaagctggca gccaagctac catctttatt gaagtccact 33720
tgagacatgt atgccaaaag cgtcccttta tagtcgccct tccgctcgct tttagctacc 33780
catagttgct tcgatatatt gaattgcatt tttcttccct cctctgttgg atgccgtaag 33840
tttacatatt ccggccaacc ttgtcaaccg caaatttcag gcaaaaagaa gcccgccttg 33900
tgggcgggct gaaagtgtgg cgtccttgcc actaaccatg atttgaatct ctacctacaa 33960
cacacctaat tacctaatta ccaaaggtcg tctgggtagg cttggccgga ctctcgaata 34020
gccaagtggc gttctagaaa ttcgatatga gcatcaaacc atgcacggat gcgttggttt 34080
ggtgcaaagc gtctagcttc tttgaactct tcgagttcct gccgcagacg acgcaccgag 34140
tacttacgat acacggcctc tttccagcgg ctagtcattg ctagtttcct tatattgagt 34200
agggaatttc ctacatacca ttgtaccatt cgtcggctaa ttagtcaatc tcaggctgcg 34260
gaccgggcca tacagcctcc gggtctcgaa tccaagcctt gatctcggcg agtgtcttaa 34320
acccctctga tttaatcttg ccttcgcatc cgtaagggta gtaatgccac ttcttaccct 34380
gtttcccaag ccagcctagc ctcttcttgc cgaatcggac gattactcga ccgtccggag 34440
tgacaggtag gaagtttacg cacttgacgg ttcttgccat ttcaccactc catgctgaca 34500
gtgacggtct tagtcttgtc gtacatgttg cggagaatcg cttcccagcc cactgccgtg 34560
ggatatacag catttacctc gtaggtttta ccgtcagatc gcagtcgaac aacatcacct 34620
gccttggggc gcttgtcgag ggacagggtt tctacttctt taggctgtgc tttcatgagt 34680
cctcctcaca tcttgctgaa gttttgtttg tagtcattgt acacgctgtc gaacagattc 34740
gcaatagcct gaatatgtac ttcagacggg gtgccagccg ggagatactt acctttcggg 34800
gtaatcagag aggttgcttc ctgcccgttc tcaccagtat acgtcacagc cgcgtttact 34860
gcaatcttaa tatcgcgggc cgtagcctcg cccgggcgca taacctgttt cacttcgcta 34920
agaacacggt ccttggtggt gaattcacaa gcaggtttgc ggtcttctac cttgatgcac 34980
atgctataca gcagcttctc gctcttcacg gagcgaccga gattgatggt gccgtcttca 35040
gtagacacga caatatcctc ggcgggcgcg ctcttgttgg ccaacgcagt aatcccgact 35100
acaaaaccta ttgctgcgag agtcagagcg acaacgacaa tcaaagcctg cacaatctct 35160
ttcatattca tcagagcacc tcctagttaa ttttggaatc cagtacgttc aacacagcct 35220
cagcagcttc acgaagcttt acacggagct tgtcgcagtc ctcgatactc acctgctcat 35280
tcatttcagc catagtgata agcccgtgaa tataccctgt gcgattagat aacgccctgt 35340
cacggaagtt ctccgagtac ccagttacgt cctcttgcaa gcgttcgata caggccgaga 35400
catactcgat gatcttctct gttgtagcca tggctcctcc tctgtctgat gtggccatct 35460
tactagcact tctattcgtt gtcaaccctc ttgctgtctt cgatatcctg tagaagctgc 35520
atcctgacga acggtttatc tttcgttgcc ttctcgcaat cagggcagta gatagtgcat 35580
actcctcgga cgtacagttt acctccgcag gcgtagcaca tgttaatgcc tagcgagtct 35640
acaacattag gcttcatatg cttcactccg gctcagtaca caagctagta ggcaggggct 35700
cttatcgaag ctgccttgga aatgcccata gtttgcagat tccccgcaac aagggcaaac 35760
gtagcccggt agagtataga tagcttcgtc ttgatacacc cattggatat cttccagtag 35820
ggttttcacg acacgcatat cacacttagc gatagccacc ttagccttga gttcggctac 35880
ctctttttct aactcaccga ctcgccttga gaaagcgtcc cggtcataca taacttgcat 35940
gtatttatcg aacatatcca ttagtctacc tcttctagga tgatgtagcc cggatacagg 36000
aatacttcag cgataatccc ttgacgaact ccgagttcaa cgcctgtgac accatttaca 36060
tggatttgac catgcttgtc ctttgtccac tgaacgcctt taagcaggtt ataggcggcc 36120
ttctcgcaca aggtcagaac gtgccgacct tcgttatcaa gcacaatata ccgagattta 36180
tcaatagttt gttccattat actacctcct caaaagttgg aactaagatt cgttgctgtc 36240
cattgaatac cggtttatca ggctctacgt cattgtactc ccagatgctg atgacgcgat 36300
tatccgcagt tacgcaagtg aattcgcagt acttagcccc tccccacgca atcgctacgt 36360
ccatagcttg cgtcgctgaa tcgaacggtt cactggtctg cattagtcgc tttttaaaat 36420
attctttcat taaaacacct ccagtcctgt ttcgcggata gcttctttgc agcgattgat 36480
tgtgtacttt tggatgtcga taaatccgcc ttctggcgcg ggaagctcaa cactcacttt 36540
tcttcgacta gccatccaga tatattccgc ccatcctttg gcacaggatt tacgaagttc 36600
ttcttgctcg tcctgtcccc accatttatc gaactctttg cggagtttgc tgttcatcgc 36660
gtgaactcca caacccatac agggttgatc ccgccagtga agtctcgacg gattgtagca 36720
gtccatccca tatctatcaa gatatttgta cgagagcacg ctgcgctcca agtgtagtac 36780
acttcagaga atttgttcat tttagtccct ccttgttttg atgggctcag aatacacaaa 36840
ggcccggaag ctgtcaagcc ccgggccaat gattttacat gcacatttca cattcttctg 36900
ttctagagat atccccagca cctcgcatac tatagatgta gtatagcgac aggatgttag 36960
ggtcttccat ggccagacga tggatgtatg aaatgtaagc cggcgagtcg ttcgacgtga 37020
agtagaggtt gatcgactgc ccttggtcga tgtacttctg ccgctgagag cacagacgca 37080
ggtagtcttc cattctcacc tcgaaggcag tacggaacac agccttttcg tgatctgtca 37140
gccagtctac gtgctgaaca ctccctttac gttcgttaat ctgccttgca cactcttccc 37200
aatcgaggcc tcgctcttgg ataatctcca gcaacacctt gttgacacgg aagaattcac 37260
ctccagcgct ctgcttggtg aagcacatag caacgtccag tccgatgccc tcagaagccc 37320
cagccatgag ttctgccgtc gatttagttg gcggcatcat cattgtagtg gcattacgct 37380
tccctagtcc acggcagccc tctggctccc ctagaacctc agctagccaa ctgttacaag 37440
cttccgcttg ctccttcata cccttgaaga tggcgttgtt caggaacata gcatccatac 37500
taccgaccgg gaatcgctcc cgctgtagca gggtgtggaa ccctagaaca ccgctgccaa 37560
gcgccctgaa gtccttggtg aatcggtaaa tcttctccag tgctcggcgg tcctgaacgc 37620
tgacctcatc catggttgct aggtattcgc tgatgttgca atcagacata acctgaccaa 37680
taaacacaag gtgcttaggc caagaacggt aaagttcgag gttgtagttc aggataacgc 37740
agctaaaagt ataccagtca tttgccggga gaacagtctc ttgacaaagg ttggacgctt 37800
cacagcgtag acccgcccgt ttaaacggct ctgcaaggtg ccgattcatc ttatcgataa 37860
acgtgaagta gccttttcct ctggccagct tgacgaacat cattcgcttc catcgcttca 37920
aggtctccgg gtctttctcg gccatatcct tgcaccactg gtcgtctaga agccatccga 37980
cgttgttcga ctctgtgcgc ttatacaggt gatcgataac gttccagaag tctccatggc 38040
gggggttgat gctgtatgca cagctaccac gcctagaacc ctgtgctacc tcttccatga 38100
cgttgataac gtcccgaatg acaggcatca caccttgaga ctcaccgcct cggatagcgt 38160
ctccttctga tggccagttg gtcaggctaa agcttgtgcc atggctgtgc ttcgttagca 38220
ctgcgatctc tgtcattaca ttgtaacggt catagaggtt gttgtccatc agaccgccag 38280
cacaagatac cgtagtgccc ttcttgcgga gcccgccgtt cgacaggagc ggagtggatg 38340
ggctgacaaa tccatcccac ataacgtcga agaatacctg ttcccagtcc ttgcctctag 38400
tgtattcgtc ctcctcccac cactcaggat aaaccgtggg ggcgtgctga gccatagctc 38460
tggctacagt ctcaaaacga gagcgaacag attcgccctc attcgaatat ttcttcatga 38520
acaactggta gccagccgta gtataccaca gaggagtctc tcctgctgct tgactgtttt 38580
tcctagcttc ggaatagttg aatgtgtctc tcttactcat atttcagctc ctcttcccaa 38640
gcctttgcta gttcctcttc atcccacccc agttcgtatt ccatgcccat tcccggagta 38700
aagaagtcaa tcattttgta agcgtacgtg ttcatttcga accaatcttt gatcggagtg 38760
tcatcgttct cgaaatgatc ttccagaccc agacgacggc accatacgtt caaacgataa 38820
cgaacaaacg ctttatagtt ttcagccgac tcgccgttaa gttctccgcc cgggatcgcc 38880
atatcgacaa tacgacattc atgagcatat acatggtcga tagcttgtcg aatcttctcg 38940
acacgccgag tatcttcaga caatggtcga ccaatctctg cgtagtattg gttgatgatc 39000
tcagccgaaa ctacaccgtg caagtcttca tccacagcac tctggttagc cccgcgtgcg 39060
atgacaggga tatcgttata cccgttgcac tggaagctct tcaggatagc gaaagaactg 39120
aacagaaggg cagtctctgt taggctgaag ataattaccg aaagaacatc gtcttcatcg 39180
cctagaacag actcaagcca gtccatccga cccataagct cggggtcgtc cttgtaagct 39240
acatagtccg catctgtatc catccccagc actacgttga tctggttgta gaattcagcg 39300
tggactgcca attccatcat gcacaggatc gaagccgcta gtttaacctc tgggcgaggg 39360
aacgttcgga ctactttacc catccaaaac tcctcaccta cgatcagttc gtatttgagg 39420
aacagttgaa ggacagtctt tacagcgtga agctgatgag gttccaactt aaacttcagg 39480
ttcaacttat ccagctctac cttcatttcg ctagagaacc acaacttctc ttctagttgc 39540
tggtttgcta gttcgacaat gaccggatat ttccgggcat aggactccgt tggagtcctg 39600
atttgtgtga tcttattgaa aatatcggcc tcatctacga gagcctttgt aacttccata 39660
tctactccta ctacattaat tgtggaacgt tgcctctgtc gatccctctg gtcgcatctt 39720
acgaagagtg aaccttagat tactatcatc acacgctgca atatgagctt ccagagaggc 39780
gcaaccgagg ctcagccact ccttgtctgt acgctcaaag ccagagaatc gtagaccctc 39840
tacgactcgg ccaatcaggg tgcgatgctg acagaaaacc atttcatacg agtagttttt 39900
aacatccatg ccgttagtcc acagatgctc ccgcactact ttttcattat tactctggaa 39960
cgcctctttg aagccatcga ccagcatgag gtcttccaga ctcaggttat attctgccat 40020
ttttaaactc cttcctcttc ttggccgtag tactcattac atacgtgccc tttaattagt 40080
tgacgatgtt ggatgaaacc tcggaagtta ccagaccaga ggtcaccata ttggtccaag 40140
tgcgtcacac cgtcctccca tccagtttga ttgtccgaga tgcggagcat cggtgttgct 40200
tggtgttcga acgggctagc gtgaacgggc ttagtatcta ccaatcggtt gtagattaca 40260
agagctttct ccagagtctc gtcgttcttc ctatagctaa cctgagcaca gcagctagca 40320
gataccttaa tagcatcctc tacgctcagt tcttgcccgt tacaatctgc gtaacgcagc 40380
ttaccatccc ctatgcggaa ccgagtcaca taaggaacat gccactctcc gtcatataga 40440
gcctctgccg ggcccgcttt aataagagcc tcatacattt tctcagcgag aaccttgatc 40500
tccggttgtg cgtctttatg gcaacgcagg tagaagaagt tatctagttc cgtggctgta 40560
acaacggtct tcatcatctg gaacggctca aggagacggt taaccacctg cttgtggagt 40620
cccattttat ttagtgcgct agcgatccga gccgcactct ttgcagcctt cttccacagg 40680
tactgagagc tacgttgcaa acactcactc agttcgaact cagcttgcat acccggttga 40740
ttcattcccc agtgaacagg catcgcagga ttctcttcca cctgtcggat catatgggca 40800
acgggaatgg cacgactact tgcagcattt cgactaaaaa gtcggtgagt cataagttca 40860
ctgtgaataa accgaggata ctccagttcg aatgtagtga tgaatctgtg attcttcgta 40920
cctaggccga tccgggaact gtgttgaatc acttttgcag aaatttgcat aaactctcct 40980
tattaaatct ttgatacaat aaagggagcc gaagctccct tgttttactg aagcattgcg 41040
aaggtgaggc accagacgat ctcgctagcg aggataggat gcaccatcat gagcttctgt 41100
tttactttct ggatcatatc tacacacctc cttcgtttca acttggagcc tattatcgca 41160
agcctcgggg agaatgtcaa cccctttctc tgagaatctt atcgatctcc aactgagcga 41220
agaagatgac tttctcgtag tcccggataa catcgttacc ggccttcagc ttgcctaggt 41280
tctgtgctgc cgcagcccgc cacaacgctt tgaacatgtt gccttgaccg aatcgcatat 41340
ccagcgcttc gatgatatcg ttgcactctg caaaatacgg catgttagcc ttcgtagtgg 41400
ggttgttcac cttgacagtg tagtaactag aggagcctcc gctacctaca gcctccttgt 41460
ggttagccat agcttccaat gtggcatagt tttctttgcc taggtaatct tctaggttgg 41520
cgaacacaac cttcttaccc agtgcaacaa gctcttcatt gagtctgtcg acctcggcta 41580
aagcctcgtc cctctcactt agttcgtcaa cttctttgtg ctctactgtc tcgggattcc 41640
cagacatagc tgaaagttcc tcatcgtcgg cagtgtaagg cgacagaaca tttttaagaa 41700
cagccatgtt gaatagtggc atccctttag ggtcgccgcc aatcagaaag tctccgtagt 41760
agcgctcact gtttgtcaaa ggctgagccc gatcccagtt aacaaaggta gtcactgggt 41820
gatcttcgaa cagaacatca tacgcttgtt taacgtcgcc gagactggct gcgtcataac 41880
ccctctccat taggaggaag aaatcagaga atctacatac cttaacagag tgctcatcat 41940
ctgcactact agccagcctt acgaccaatt ctccctttcg tatatcaccc cttttctcgg 42000
taacaaccaa gactgttccg taagggatgg tatcggcgtc ccaatattga atattgatag 42060
gattctttga ccgttgcacc agagaaatcg gttgcccagc atgaacgttg atgatatcgt 42120
tactcaaagt tcacctccat atctttcaac tcttgaaacc cgtcaagact ctcgatctta 42180
gcgcacatca ctctcatagt gacatatccc ttcagtacac ccttcggacc tttaaactga 42240
ctctctagga aggccagcag ggcatctagc cctgctgagt caaccatgag atcgtcgaca 42300
gataggtctt cttttacaat ctcttcggcc acttgttact cctcgtcgct aatcgattca 42360
cgaatgcagt ccagaatgaa ggacaggttc atgtgcagag tcaggagatc gtctttggtc 42420
tccggcatcg cagcaaccaa gatcatcagc ttcaggaagt cagggccacc cattaccaga 42480
ggcagatcac ccaagctggt gcggatgtgt cgtgcaagca tgccatagtc cagttcaccg 42540
tcctcggtca cgatggaatc ttccagagat ttacggtcgg tcaaggcttt ctctaccaga 42600
gcccgctgct cagggttcat acctgccatt gcttcttcca tttgctcagg ggtgatgttg 42660
aaatcagtca ttttatatct ccttttcgat tatttagatt tgcggtcgat tttgaaaata 42720
gcgccgctgt tgcagtgtac agcaccgtct ccgaagaagt caactccgaa atacttgacg 42780
ccttcgttat tctcgcatgt tgaagtcact ttctcaatat cttcggcggt aaagctcgca 42840
gtgatactaa acataaagaa caagcctgca agggccgccc caaggaaaaa ctcagccata 42900
tgccctccta ttacctaatt agccgataac tttaacagag tcgaagccga gcaggcctgc 42960
gtcctccagt tcctcaatag tatcatcaat cccgcaatct gacaaggctt tagctacatc 43020
ttcttccatc tggtagtagt ctcgccacag gcactcattg ataccgatat cccattcaca 43080
ccagaactta taaaccgtca tttgaaacct cctttctcca tcatgaagtc taggcttacc 43140
ggcattggca gaccaaaacc atccttcact tcgtgaatca taacacaacc ccggaagtga 43200
ttgttaccct gatagccttt gtaagcctcg tcgtgaggat agcacgctcc agtcacaatg 43260
cctagccttt gttttccgtc aattgtagga tggatcgccc agtccagaag ctgacggtgg 43320
cccatgacaa acgaatgacc ggcagcctta agcatgttgt tcgctgtgcc acccagaggc 43380
ttcccagtga agtggttttg taggtagtgg acgtaataga tgccatccac ctcgataggc 43440
ttcaggaacg gaacaacctc aaaaccataa tcctgaatac ccagttcctc acttccgacc 43500
gtgccgtaca attcggggtt gtcgttggcc gctcggtctg ctcgctcttc gtggttaccc 43560
agtgtgaaaa tcaaccgagg ttggtacacc tttttccgat tagcccgttg acgctcctgt 43620
agctcccaca gaggcttgaa cagcctgtcc agaccctcac gtccgacttg aacgtcttcg 43680
gcgtatctcc ggccctcaga ggagcgttta cccttatcat aagacgacaa gctggcgagg 43740
tcaaagtgat ccccgatgtg aatcacaaca tctggacgtt tggctacgat atacttaccg 43800
atagcttcca tgtagtccag actgataccc ggcttgcatt gtgtatcaga gattacaaag 43860
tgtttcttgc tgctcattct acttcctccc actcgccctc gcccggttga taatacaaaa 43920
acgcatcaat acgtttgtac aagtccgtat catatagtcc gtttttctgc atcataacca 43980
aggcctcgtc gagcatagag acagcttcga tcaagtcccg aagttctgct atagttttac 44040
taatatcgcg ctgcacctta ttcctcctcg cactcttgac cgtagaggaa tgctgcaatg 44100
tcctcgtata catctgtatc gtagcaatgg acgttatcta gaaggttgag ggctctttct 44160
agaagcttcg ttgcttcctc cagctcctgt ttgatgaaat acgctcgggg gtcagtcatg 44220
ctcattttgc ctccttcagt agtttgatga tatcgtcgta tgtggacgtt ttaatggtga 44280
gtcggacata tccgtctccg atgtggtctg gcacttcgaa cccaataaca tctttcagta 44340
gatcgagcac caactggctt tcgtgcttca gggattcagc aatatcaaca atctcgaaag 44400
ctgattggtt tttaaaagat gctgcttccc agccggccag acgtgcggga gcggccccta 44460
ctatcaaagg aatgttcagg tcaggccacc gttccatatc tcttttagca tagtacgctc 44520
ctagtgtagt actctcatat gcgtttctcc aagctcgggc tgcccctcct ttcgtggtcc 44580
attgccgctt tcctttagga gtggtccaaa gctctttggt ctttttgtgg cgtataacat 44640
acgtagctaa attggtcatg actccaactc ctctcccgtt ttaatgcact ggtaagctcc 44700
cttggcagct tcgatcaaaa cagagtatcc gtttcttaac tcttcttttg cattatcgaa 44760
gaactctaga attccaacga ttagcgcagt tgcaaccggg agccagaaga acagagcaat 44820
ccaagccata acaagggaag cccttacccg gatgggcagc ttccgcataa ttctcaaaaa 44880
cttctctctc acagaggcct ccataatttt gcaaatttaa gaacattcgg ggtctcctct 44940
ttgctcagag gaggtactac taggcaggag ccttcgccat tgcagatggt tctctcatac 45000
ccctcatata cgaaagggat ttgcttgatc ttatcgtact ctttgtcaga aactcgtaca 45060
acgatcttac ggaagctcca ctgtttccac tccacccact taatggcggt ttttgccgct 45120
gcatgcatat acggacggga cggaaggtcc gtcgatggaa cggcccggtc ctgtccgagg 45180
agaaccattg ccatgtctgc ccctaggatg ctatgtgcaa ccagagtcgg caccatgtac 45240
tctggtgcct cgtctcttac tacaatgtaa acccgatcct cgttcatcca ccctccttct 45300
tagacttaag gtattcaatg atctgttctt ttctaagctt tgcgttagat gcaggcttaa 45360
ttccagcttc ctgcaagaag accttatcat accccttctg cttctgtatt gcaatagcct 45420
gtttcacgat atcagcctct tctagggtca gcccgtagcg ctcggcgtat gtcttgatct 45480
catggcacgg tttgcacaag acagccaaat ctcctatgcg gacaaacacc acaccttcca 45540
cgaacgcttg gatatcctct actcgcttca gggagaactc ccccgtcttg tggtcaatct 45600
caacgttagc ttgaggagtc tccttcccgc actgctcgca cacatagccc cacacagtag 45660
ggtgcctaag cttgctcttt tcactgggat tggttatcct aatccttcgg gatttagcaa 45720
actcaagctt gactgggttc ttagaccaga gatagcggcg aatgccgcct ctgacccaat 45780
tcaagaacgc cgattctgtc tcccagatgc ccgggaagtc ctcccaagga cgcgccctta 45840
cgctcattct gcacctccca aatccactcc aagtttagcc aaaacagccc tacagtctac 45900
ccgatccccc gcccaacgtt gcatgtgggc gcagtcacaa tacatttgca tgatatcgat 45960
tgcatcttta gtgtgctctg tgtcgtccca tgctgtgtaa gtgacaggct caggatacca 46020
cgatttgtag gtgtcgtaga ttgcttgcca gcactccttg tccgttgtgc agggattaat 46080
gatgttaaac gctgccactt ctccgaagtc cttcccgatg cacaggtcag caggtcggta 46140
gcagtcaaca gggtctccga atagaagctg gaagtagaag aacttacgtc cctcgcctct 46200
caccttgccc ttagagtccc tgtagagcga tcctaggcct gaaatgaact tgacttgaac 46260
aggctcacgc tcatagttgc aaagccatcc tagggtgcca tctgcgtctt tatcgatggt 46320
tgccccgatc acacgttgag tggtttcgcc tctagagtaa cttacaccgt tcttgtacat 46380
catttcggcc atcaagtcgt ccgcttctcc gggatggatg atggtgtcat acgcttcgat 46440
catatactgc cttagctgac ctagctgaag aggcttaatc tgccctgtac gcttaccctt 46500
gtacctgcca ccacgagtga aggtcttttt acccttggta atctcgtatt ctttcgggag 46560
tgggatggcg tcacggaagt tatcagggcc tgataggaga atcttgaact gcttgcaccc 46620
agcttggcgg cagattcggt cgatcatggt cttaacaaca gagataccgt aggacacgtg 46680
cttggggtct tgatggtcct caatttcgaa gttgtcttcg ttgaatccct cttcgtcttt 46740
gttattctcc cgccaagtcg tccgattctt ccaagtttcc tcttctcctg tcaatttgtt 46800
tctgacaatg atggagcggg tctcgttgac acttgcacac ttaaaggcga taaggtcgcc 46860
gtcgatgagg acgatatcct cttccttttc actcattttt atctccaatg aaaaagggag 46920
ccgaagctcc ccttgggtta gaactgtaca acctgaatgt cccactctgt ttgtagagtg 46980
tcagggttta ccttgttagg aagagcaaca taagccggct ctcctttaat gatacactcc 47040
gcagcgttct tcaaatcttt gaagagattt cgctcccaca ggtcaccgct gaactggttg 47100
tttactgtgg ggccttttag aatgtactca ggtactcgaa caataaccaa actactcatc 47160
attagtcctc acttattccg gaagtgcttt ttaaagctaa agttcagttt agtacgatcg 47220
ccgaatgcgt aaatcagacc tacaattaca agaaacagta cgagaatcga caatacaatt 47280
acaagaggaa tccagatggg catgagtgcc acagtccaag agatagcgag aactccggca 47340
gatttcagaa cgcacaggac gattgctgcg agaaggaaga tgggaccaaa attgatttgc 47400
atgtgttgcc tcctttgaaa ttttgaaagg aaggggccga agccccttgt cttaccaccg 47460
ccctttagaa cggaatgtca tcatctacgt tggtttgaac cggctccact ttaggtgcag 47520
cttgggtctg cggcttatct tcagcttgct cttcgctgtc gccactggag cggttaccac 47580
cgcgtgcagc ttcaacggct tcgatcatcg ccttgatatc cgaaccttcg tagttcttgg 47640
ctcgcttgat ggtgttgatg acgttagcac gcagttgctt gactgcttct tcgtcgttct 47700
cgccatacag gttgacaccg tagatgtact tctcgtccag ctcgggaacc ggaacacctt 47760
caggaaccat accgaccaga ctgatatcct cagtgaggaa tttcatatcg ttcttgcccg 47820
gcttcatcca cactcgcact tggaactgag ccaccttgcc cagaagctcg cctacgcggt 47880
ccttggtgaa gagttcgtct tcgttgatga cgccagtagc cacagcaagt ttgtgcaaca 47940
tggagttctt ggcgaacgcc catttgccat tcgggtgctt cacttccttg atggtaaacg 48000
gacggccaac aatcttagtc ttctgacccg gcagagtaaa gtcgccgttg tacaggacac 48060
gaagcggaga cttgttgctg ttaccggtca ggtacaggct cttgtctacc tcgatctgag 48120
ggaagtccac agtgatcgcg atttgttgca tatcgcgctg cggccagcac ttcagacgta 48180
caggcttgcg agtctgcttg tccagtccat ccttgaagta agtgttcgga tattccttga 48240
tgatttggat ttcatcttcg gcagtgccag tgaacacaac ctctgcatcg ttctgctctt 48300
gctcgccgag atcgatgata ccggagatat aacccgggat cgaacgcgct ttagcggcag 48360
tgccagcagc ttcaactacg tggttgttca gagcatccca atcgacttcc tttcggacgc 48420
cgccgttgcc ggattgagtg ttggtggtta cgttcagttt aaaagccatt tcatttctcc 48480
tatttaagta ccctagactg ggcgagtgtg tcagccactt gactgacgat cataccggac 48540
attcccggat tctgcaagag gagaagcgcg ttaacgcacc tcctctcctt tgattacttt 48600
tacaatcgag actccctcag ccttgccacc ccaccagcgc ttggtggctc gggcttcttc 48660
gcgagtgagg cagaatttaa gaggactccc gtctgcatcg agaacagcat aacccattac 48720
agcaggcttt gcaacctttt ctttcttctc ttcgtcaacc ggagcttcaa ccttgccctc 48780
cgcctcttct ttaaactctt ccagtaccag ctcagggtag agggtctgga actgcggctt 48840
gtcagaggtt tcaccgccaa tgtcaacgcg tcctttaaaa gtcatgcaga acgggctctc 48900
cccttcgtgc atgctcttca gccagcacgg ctcgaaacct acgtacaagg ctggctccga 48960
aggaaccaag taatggccga tctgtgcgct gtgcttggca acgttcttaa cgtaatactt 49020
cttgcccgga actgctacaa tttcgaagcg gtcgttgtca gcgagcatcg attggtagtc 49080
tccaagtcca tcattgacgt ctcggtcaat ggaccaatga agggagcaga accggatttc 49140
cccttccggg agaccgatat cgcgtccatc tactgcgact gcgtatgcgc ctacatatcg 49200
ggcttccttt ggccagccaa cggtgacgtc acatcccact tgagtgagtt tgacgatagt 49260
cccgggaata acaccagaca tattatttac ctcctttctg tttgtatttg ttagccacgc 49320
ttttgtgggc taacggtgtc tccgatacct cgaagccttt agaggtatag tactcccgat 49380
tggccttgtt gtcaacagtg attcctacca tggacgattt gccgtagacg tagagtcgag 49440
ccgtgttcat accatacctc ctattctgtg attaccgtat cactcgctgc tgccttcaac 49500
agccttcaga gtttctttct tagcccgctt aaccagagct tcgagcagtt cagtagattc 49560
gctgagaacc tgctgagtag cgtctgcttg atagaggctc tgtgcagcgt acccgccgac 49620
catccaccat gccgtctcag acgaaggcac cagatggccg attacaatga taggcagaaa 49680
cagcttgaac gtcaagggcc gtaggtgatt tttgagtgtg gctttaactg ccgaattctc 49740
tcggccacca atggatgcca agtaaacgaa ccctcctgca acccatgcga tgatggtgac 49800
gatcagggca aagctaccag ccgacgacaa ggctgggaat acggtcatta ccagatagat 49860
aatgaaagcc agagtcattg tgtttctcct tgtatgttgt gaatatgtgg ccattgtata 49920
ggaggcggag gggcctgtca agcccctttc ctcgcttaat gccagtttta gtggcaatcc 49980
ttccaagatc gaccaacgat gtagccagcg gtcagctcga taggcaccag aggagtagag 50040
tagaactctc ccgcagctct cacggccttc gtggcaagtt ctccagcgcg gcagtaagct 50100
acaaacaccc cgccttcacc tttgctgtag tgtgcgatat cactccacac cttgccagta 50160
ctagccaatt gttcgtcctt gtaagcctga gctagagctt tcagcttctt ctccgcctcc 50220
tcctgttcct tttctccttc tggcttaccg cctacaagag accaagggaa catcttgaag 50280
ctgaccgact tagctgtaac ttccaactgc gcttcgtcgt ggtacgcgat catttgttgg 50340
cagaattccg gcttgttctt ccagtcttct ttgaaaaagt caaccttcag accttctttc 50400
tccagtagta catcatgaat caccatagcc cgcttagcac agataacacc gccactctgg 50460
aacaagctgt tcaggatagc gtgagccgaa cgagtaggga ctcggcgacc gtccactcca 50520
atgatatact tcttgccatt ggcttcccat tgcttggtga ggtcgtcctt cagagctttc 50580
aatgggaatg ctgcatccca gaacgcatcg aataccagtt gacccgtttg caaatcggca 50640
ccaatggttt ttgccacttt accagcttgg gcaccgtacg tacacccgta ttttacgttt 50700
ttcgcaggag atcggttaaa ttgacgacca atgatctcag aaatacgctt agccatcatt 50760
gtgtgaacgt caaatggctt ctccatcata agagatttgc aatattcctt tgtcggatcg 50820
tactgatcgc agtaggccga ctctttacgt gcttccaagg aatcgaagtc gtatccaatt 50880
tggaagaacc catctccaac cccaaacaac tctcgaagct cagccccgta caacgaggtg 50940
atccgaggaa cgttagcaac cttccggtgc ttcatccggc tagtagcagc cccgcaagtg 51000
tcagcaggcg tgggaatcct gccgtcctga cgaatgcttg caaggtaacc tttttccggc 51060
tcctctccat cctccatttc gtcccagtcc atgccgccac cgaggattgc gcttcgccgg 51120
tgcttaaagg tcaggtactc aatcacatcc ttcgcgaacg gaaactgttc tgcaatgcgt 51180
tcgaggtctg gacacatttc tttctcttgg ccctttgtga agctagggtt agtgagaacc 51240
ttacagctcc ccttgcttgc acgatcaatc aacctctgac gtacatacgc gcgggtgcta 51300
cgaggggtga tcttcatgta atccccgaac ccgtccagcc gctcttcctt gtacgctgtg 51360
ctatacgtta catccagcca gcgatcgatc ttagcaacga tgttttccat cggaagcttg 51420
atcttcttct gatccacagt cagatcagtc tctttccact ctgatggaat ccaaccaaac 51480
tcacccataa gccattgctt gatgtgagtt gtgtccccga tcttagcagg catttccgtc 51540
ttcagcggta cgtcagcggg aagcggaagc ttgtactcct tgccttcgaa gatcatcgtc 51600
cggtcgtcac cctctttcaa ctgagcccca atccgctcaa taaacttctg caagtagctg 51660
gtgatctccc cgttctgctt gaactgccgc ttaggcggtg tgtagtctgc catgaatttc 51720
ttggtagctg gtcgaggagg aagcacaggc tctacccggg cccgacgctc ttccatcttg 51780
gcgtccaagt ctttcacagc tttttcacag agatcaacat tcagaacaaa ccctcggtgc 51840
tcctgatagg tgatgatctc tgcaaccttc ttctccaact gaatcgggga cagccaattc 51900
ttgatgtagt tgtcacggtc ggacagttgg taatcttcgt ggtcctgcaa ccaatgccat 51960
acagcggtgt tcgcctttac gtcatagata cagtagtaga gcatatcggc tgcgaagtcg 52020
aggaatcgaa catccggtgc caaatgctta cggaagtcaa tcttggtgtc accacctgca 52080
cgacgagaca gcttatccag actgtgaccg ccgaacaggt cagggttaca agtcttggag 52140
ataacgaggg tatcgtcgaa taccacttgc ttgcccatcc aagtgtcagc agaccacgta 52200
gtcagaccgc catccctgtg ctctacgccg atagtgaagt ccatatcttc cacaagcttc 52260
atcgacataa ggtcgaagtt aatcatgttg tgagccacta cgcgcttgat cttgcgcttg 52320
agcacatagt tcttgaactc tttcagcggc aggtgaatat actctaccgg agtgtagtcc 52380
ttgagagtca caacttcttc agtgtggagc atggaagacg ctttcggcag tctctcagtg 52440
tactctcgcc cgtcaaagac gtaggtcggc ccatcgtaga atgccagaat attgccaccg 52500
tgctcctcca ctacaatgca gtggtgtttg tagctaggct taatcttgta ggggctggca 52560
gtgtaatcga tagacgtttc atccaacagg cctgtggcct cgatatccca agtcatgttg 52620
gtgaaattgt tatttcccat ccattcgagg tattgctcga ttgtttcaaa tcgttgttgc 52680
atttcctttc ctcctgtaaa cgacgaaagg gatgatacat aagcatcacc cctctgtcaa 52740
ccttagtatt ccgtgtcccg gccttcctct tccttctgag ccatccaagc ctcgtattca 52800
cggcgcttgt cagggtatcg gtccagatag tcgtccaagt cccagattgt atgagtctca 52860
acgtcgtagt accaattgcc catcattcca gtgcggccag tccatcggca cttagagagc 52920
ttcacgttaa tcaggttctt ttcgaactcg ttctccgcgt ccttgttccg ccacatcaga 52980
atgttagctg caccagattt gaagatggac gaagaccctt ggaaatcttc ttcgtacagt 53040
tcagcacctg ccgagttagc cttctgacca ccaccactct tacggacgtg gttgatgttg 53100
atgaacgtca ctcggtggct cttggtcata cccttttgcc agcgcatgaa taccgactgc 53160
tcttcgttgc tcaatccgtc cagaatatcc tgaagcgggt ccagaataat aacacggcac 53220
tcaaactgaa taatcagttg ttcgatttga gccttgaggg ctgctaggct tccgtctcgg 53280
tcatcgatca gatagaagcg tgggcttccg tcttccttgt gccacaagtc gtaccgagcg 53340
tcacggtgcc gatcaaggaa cgccactttc tcttcaacgg tctcgtacaa gtctgccttc 53400
tcgcctacct tacggctcag cagcttgatt gcgtactccc cgcagtccga ttccagagtc 53460
acagtacccg gcatatgagg cgagttataa atccaaaaca gagtgaactc atccactaca 53520
gtagatttac ccatgcccga tgcagcgccg atattcacaa tccggcccaa cgggatgcct 53580
cctgccatag ccttctggag cttcttagcg aacggtggca gaggaatctt ctcaacctgc 53640
acagcttcat acattcgagc gtcgagtgca tcagacccta caataccgct aggggtccat 53700
gtacgggctc gatagaatgc actgacaaac tccgactgac ggccagcttt caaatactcg 53760
ttagggtctt tacggttcat ttccatcatg aacaccttgc ccttcggaag cttatcggct 53820
gccttgtctg ctgcctctcg tcctgcatca tcactgtcga aacagaggat gatttgttcg 53880
aatcggtcta gccactcgta ctgagcctgt agctgtttcc acgccccact ctcaccgata 53940
gtagggctga ccactggagt cggttcataa tcactcttac cgcctgctcg ccggtcatca 54000
tactccttga gcatctggaa tgctgatagc tggtcaacct caccgcctac aataagcacc 54060
ttcttgctgt tgctattctt gaacctgaac tgaccaaaaa gctcgcaatc cttcccggtc 54120
tcgccgatag gctctgagaa gttcttcggc atgatccgct gtttgtagcc agtgagttca 54180
taattagtag tagtaggata tagctgagtt gcaatctcgc cagtctccgg gtcgaaacca 54240
tgtagcactc cgaaatactg gcaagtttcc ttggacaggc cacgataacc acgcggatca 54300
tacgagcatt gctctttaat ttgagcattg atctctgcgt taaattcagt tcccacgaaa 54360
ttccactcct cttcgtcttc tccccgggcg tctctttcct gctcttcaga gagcaaggtg 54420
tacccacacg cccaacaata ggcacctttg tgttccccgg tattacgatc agtaccatag 54480
acctttaggt tgtctccgct gttgtcagcg cctttcttcc ggcattgggg acaagcggtc 54540
ttgtggtcag aatacagatc gataccgtgc atagccgcaa tttgggccac ggtcattttg 54600
gacataaaac ctccttactt cagacaacca cggagtttag catcaccttg cgttcgtgtc 54660
aacccctctc cgagacgtat tggtcccgca ttgcacggtc cataggaaca gccacctgat 54720
agccggagcc cagagtcgtg actgtccatt gatctacagc gttctctgtc aaccactcat 54780
tcatttccga gaacgtgtgg aagtagatgt atagcagatt gtggtttctc agtgtagcct 54840
tcatggcgag tcgccttctc tcatgtggtt gaatggtgag aaatccgcat gatcttcgaa 54900
cggaccgctc cagtaggtgg caaactcgac ctcggtgagc cttccgtcgt tctggtagta 54960
accattctcg taccctccac accgatgaca catgcctcca gtgacttgct gcatccatcc 55020
aagcccaacg tctacaaact cagcttcggt tttctctcca caccatctgc actcgggtcc 55080
gttcattata ccctcctacg cctgcttgga caactcagag cgctcgaaga acaggtgaag 55140
gtactcgata agctctccgc tttcgtcttc gtagctgccc acatcaataa cgcaaccctc 55200
ttcgtctact gcgatgatag tggcaacacg gccccggaag atggtatcag cgtcgtcatt 55260
gatcctcact ttatcaccct tcttcattta atcgctcctt ctgtttatgc ttaggcttcc 55320
gcctgtattt ggttttatcc ctctctaccg agggcctgtt gactgtgttt agaaacttga 55380
acaccgggtt cttcttagcc atagaatact cctgatccac atttactgca acccttatac 55440
cagatgccag tcttatgacg gagcatgaca ggctctccaa gatgcataca ccggtagcag 55500
agtcgtttca tttgtcctcc tataaacgtg tgaagtcttt cagcgggata tggaccataa 55560
cttcttggtc ttgccagtct ccacggtcaa tcctgccacc catgctcatg tagttgacgc 55620
cattggctgt cattttgaca tatcctgtat agtccgtcca ctttaccaca aggaatgcgg 55680
gaatgtcaaa caccctttgc atttcgatcc catggagcca cttcatctgg gacacaaaga 55740
agtcgggata tttctgcatt tcattattcc gagccttaag ctccacaaag ctaacaacct 55800
ttccattccg gcaggctgcc caatccatat agtgggctct aggtagctta tgaagagaac 55860
acttccaccg cccttcaagc tcagaacgga cggcctcctc gaccgcccta tctgatgcag 55920
actcgtaagt ctgtctagcc actcaaacct ccttgaagaa gacgtactcg aaggagttgg 55980
tcataatctg caacttgccc tccatgagtt tgccgtccac gccttcgtct tcatttcccc 56040
agaatcggaa acggcaacgc tcgaaatcaa gctccaagaa gctaggctca agctctcgaa 56100
tatccgggtc aagaacatac tcaggtttga tataaaatcc gagacaagca gtgtccagca 56160
catcccagcc gttccagtgg gaaatgaagt agctctctag cccacgtgct tcaactacaa 56220
tctcatcgcg cttactctga ctcatgctta cacacctcct tcagttttaa tagagaaaga 56280
atctaggaaa tcttggatat agggatcgag ttcgccaact gcctcaataa gccgcatgca 56340
ctgacgctca ttatcttctt cttctaagat tacaacgctc atatacttac gccactggtc 56400
atacaactcc attgttcacc tccagttgaa tcttggtcaa gtacatacct tgcatttcat 56460
ccaagaagta aatgagttct ccctgttcca ccaccgtcct gattagcgcc cctgctacct 56520
catactgtag aatgaggtgt acgttacctt tcctatcgta aatcttctct actacataca 56580
ctacgatagg cggggtttcg tcttgattta cactcacagg cacgtccact tctacgactt 56640
ggccagttcg tagctgctcg acggactcat tcagtcgctg agcgttagcc ggagattcca 56700
taaggtagtc gtggtcacaa ccgaaggcct cgtctagggt gcttacggga gcttttgctg 56760
ccttacatgc gatgtaaaga tcagaagcgt caacaactac aacagccctg tggtcagtgt 56820
aggcgtctag acgtactgca cagcactctt ccttcagtcc cagttcagtc atcacaacgg 56880
tgcctttcaa gcctccggcc agttcgtcgg tgtggaccca actgtcttct acaaaccccg 56940
tagctagcag ttcatgcaaa cgaccatgcg cctgcgccca tcgctttgca catccagtgt 57000
atgtatcgca ataggcctta aggaccaccc gatctcccac ttcaacctta ataatctcgc 57060
tcatgcttta ctccctctgt tgatagctcg ttgcttcttt gcctcaaggt aggcgtcgta 57120
ttgctccgct gtccacgggg gaaggtcatt atccacgctg aacgggttgt cgtcaaccca 57180
tacagagact tttattccaa gattttccat gaatggtcgc tttgccttct gtgccgtgaa 57240
atagatcggg aacccgtagt cccgtagcca ctctaactct ccgctgttgt cagggtggcg 57300
tgcagtgact acgatcacat tgaaagcgcc tccaaagccc ctgaaggtat ccatgacgat 57360
tcggaaggca agagggtaga gactgattgt ctcatcataa tcgaatgcaa ttacaaaagg 57420
ttcgcccata aaacccgcta actgatccat gttattcacg cccctttgct acgtactctg 57480
tcgtaggagg tgctacatgg gtgacaggga ctgcaaccca agcagtcacc tcttcgtgac 57540
agacagggca ttctgcggag tagtatcccg tctctccggg acggcagaat gagaacgaaa 57600
catactcaag ttccgacctc ttagcccgga gaatgctcaa gcagtggtgg cacttagccg 57660
taaacaaatc ctcttgaggg agcgtccctc tcgaaataat ttccataaat cctcctcttt 57720
gtacttcaga tgtttgtgtt tagacatacc agtccggggc ctttctcaca ggaggtgaga 57780
ttggatcagg cttcctctcg atcttctcag gtgactcccc gggccacact tgccagcagt 57840
gccagatggt gcgtcttttg tcattagtgt gatcgtacaa cactccgtcg atgattgcaa 57900
cagcatgccc ctttaccgcg atccagaacc gaccttcagg gttttcctca gcaaactgcc 57960
taagagtctt atccttagca tcctcccggt agagcatccg ggtcttaggg caccaagtgt 58020
ctgcgtagct gaagcgcttc tgagtagccc cttgccggta accgcgcttg cacttcactt 58080
tcaggtgctg gtgtgccttt tcataggtgg tgttccatac gactgcaaag ctcaccacca 58140
tgcagtcgtt ggtttcgcca tacctacgag aagcgtcgaa gaacctaggg taagggtctt 58200
tgaaggtagc tttcgactcc tccacggtta cgcacctacg gttgggaagc aatctgcaag 58260
agcttcgacc tctagctccc ggccatcgtc atcgaagtat ccgcagtcga ttagcgaggc 58320
ctcataccag tcccagttgt caacgccacc agcctccaga gcttggagct tcatacggtc 58380
gcgtaacagg tcgattaggt cttctcggta aataacatgt ttcatttata cctcctctgt 58440
gattgatttg agaatactct acacggattg cagagctttg tcaagctctt gtatgcagat 58500
agatcggtag tggaggcaga cctgtcccta ggcttgccat acggattttt ccgcctaaga 58560
catgcctctg gtctgcctct cgctctcatt atgcagatga gtcggtgagc ggggagcatg 58620
ggctccttat aggctctgag ctttcccctt tcgggtcgtc gctgccctgt tctcagattc 58680
gactcaggat gcgcttgcag ctttcctctt catacgctat actgctggcc ggtgagtgac 58740
gtggacattt ctctccagca ggtcacccac tttaccacgg attattttct tgtcaaggct 58800
attgacacag cagaggaatc cgtccagaat acaacgactt tcaacaaaac aggaggaaac 58860
accgtgaaga tcgatattcg aaacgaggcg gcagaggtca gggagattgt cttcggagat 58920
agtgtagact gtgcttgcag acttgaaaag agcgagtata ctgacgtacc tgtgtacatc 58980
gcagatggct taccggggaa tagtgggagt tggatgccga tccgtgatct tctagatgct 59040
gagaatctgc tactggcctt agagcgagct attgacctcg gctggtttaa caagtaatat 59100
accaattttc aaacaaggag gataacatat gagcaattcc caagaccgcg tactagcccg 59160
agtggtgcgt atcgacgatg tgaagaccca tcctaacgct gacagccttg atctggcgct 59220
tgttggtggc tggcagtgcg taatcaaacg aggtgagtat caggctggtg atctggcaat 59280
ctacattgaa gtggattcca tggtccctat ggacaaccct atctttgaat tcctgcgagg 59340
ccgtaatatg gttgagcggg atggaaaaga atattctcga atcaagacta tgaagcttcg 59400
caaagagctt agccaaggat tgatcgtgcc tgttcatgca gccggcagtc cagagccgaa 59460
agtagacgaa gacgttactg atcgaattgg catcctgaag tatgtaaagc catctgagca 59520
ggcagctatc aatggcggca acaagggtga ccgaactggc acttccaagc tagggttccc 59580
caacttcatc ccgaagacgg atcaggctcg cgtacagaat attcagatgg cttacgatca 59640
ggctcatcag aaggacgaga agtttgaagt gacattcaaa ttggacggtt cgtctctcac 59700
tatctggcac aaggacggag aagtaggcgt ggcgtctcga aatgtcggtt tccgtctaga 59760
agacgagaag atcgggttct tcacggctgt caagaacttc tttaagggtc gtggctggaa 59820
gcgtgtgatt aagaaagact ccaatcactt cactgcgatg gcagaagagc ttaatcttgg 59880
tgaacgtctc cgggagtttg gtctaaatat cgcccttcag ggtgaactgg ttgcaccgaa 59940
tattcaaggc aacttcgagg gagttgacaa accggaatac tatgtgtacg atatctacaa 60000
catttcggag agtcgttact atcttccagc ggagcgtctt gccattctga atacgctgaa 60060
ccagttgggt ggtgagacgg tcaaacatgt gccggtgtac atgtgggacg taggcgttcc 60120
aaaagacatg caactagtaa ttaaaatggc tagtggcgag tctgcgttga acggtaagta 60180
ccgggaaggc ttggttttca aaagcatgag ccgagacttc agcttcaaag ttattagcaa 60240
tgagtatctt ctgaaggagg agtaaatatg gcttactggg gatgcgggga ggttgatcga 60300
gttgtgtgcg aatgttgcgg gaacaacttc cctcccgagg aggttgtaag cgacgacggc 60360
atgctaactt gtgtcgattg ttattttgaa atgttctgca aggagggtga agttaatgag 60420
tagagttaaa gatcatatcg aggaagctca tcgcccagct agccgagaga ccacccactt 60480
gggttctttc gtcttggcca atggcgtaag tggagaaaaa gtaccagcgt ttgttgtagc 60540
catggagact caggaactat ccgctgagga caatctgcca ctcctattat actttggtgc 60600
cggtcatgca ggctggagac catggggaga agtgggcagc acttccctgc gaatcgacca 60660
gagacttatt gataagcgcg attgggaagg gggagctgaa gttgttaatg cgattgatgg 60720
atatgaagat ttcttctcat acattgacga acaagagctt atcaaaaaat tccacgacgc 60780
ttggatggaa tggcctttcc acactgcaat tgttgaagat caagagccga cgttcggtga 60840
actattcgaa cgagagtccg tcttcgagtt tgttagcggt tttaacaaaa tgatgactgg 60900
tcttgaggtt gttaaccgca agtggaacac cgatctggtc acaggcgagc cgattaaacg 60960
tgatgctctg cacatgctga tgctcgttac cactgaaatt gcggaagctg ctgaaggcgt 61020
ccgtaaggat ttgatggacg atcacttgcc agagcggaag atggaagagg tggaactggc 61080
cgacgctatc attcgcatca tggactatgc gaatgtacgg ggtctggatg ttgcaggggc 61140
tatgatcgag aagatcgagt acaacatgac ccgtgcagac cacaagcggg aagctcgcct 61200
tgcagaaggc ggcaagaagg tgtagaatga gcttgcaata tatggtgagt ggtgatgagc 61260
acatcgacga ctatgcaacg tatgagttga gttatcccga tctcaaagcc acgtataacc 61320
gcctgtcgaa gctggacgat tccagcttca aggcttctat gccagaggta ttgcacttct 61380
gctgttttgt agcttggata aaagagattc ctagctctat tttgctggga gacagaggac 61440
tgatacacct tatcgctcac cacattcatg aaggagcgga agacgatggg ccacaggctt 61500
ttaagtctct gaggcatcag ttcaacacac taatgaagtt agattaagga ggttttaaat 61560
ggaattattt gacgttaaga aagggcaggt tgttatggtt ggcagtctgg acatagacat 61620
tccgaggccg accttcgaaa agaccaacgc tcggatgacc ggcgtagttg tcgatattga 61680
cgagggctcg atgcatctga atgttaaagt gctgttcgac aacggggcga tggactgggg 61740
caacgcgtcc gatattaagc tactcaattc cggcccagag gctaacaaag ttcgcaagaa 61800
gctggctaag aaggttatcc agaagcttga tgacctgttc gatgaagtct atcaagttgt 61860
tcgctgaaaa cccatgacaa agagccccgg taagctttcg ctcccggggc ttaattattc 61920
ttgtttttct tttattctcc gctcaagttc gctaatcttc cttttaagcg agtcgagttc 61980
ttcatattgc aaacgggatt gccgatcctg tgtgtacgag gccgagttta tctgatcctg 62040
tagtctgttc agtttgagtt cataccgccg ctcagattcg ctaattcgaa tctcgtaggt 62100
ctggcgtaca gagtcaaacc gagcctcggg agtcatcgct gtgaaagcca gaatagccat 62160
acagacaagg agggagataa acaaagcccc gactgctgaa actagcggcc agtcttgttt 62220
ccttgtcatt atattgtctc ccagttgggt taacgggttg tagttattga gttgctgtat 62280
cccgaccctt gacggcattc ataatcaaaa tcaaataccg gttggtttct tgttgttctc 62340
ttcttacgtt actaatttca ctttcaagtc ggctattgaa tctcgactct agtgcgttaa 62400
gttttgcctc ggataccata tctctttgca tcgcaaagat acgttcttcc tgcttagaaa 62460
gactggaact taggaatcca gaccaagcca ttaggatagc aattagcaga gagataactc 62520
cctgtgtagc taccttgatt aggccttctt tgccgtcttt actttccata cagagccctc 62580
tgctcttctg cccacagctt gatagtctgg agttgactat tgcacatagc agcactgtta 62640
gtgtttagaa tgtaagcttt tgcaagcgct tttactgtat cttgttccac tcggtacacc 62700
ttcacttcat cggttaggct ttccgggggc acaataagga tcgtttggta ctgcggaaca 62760
gaaggctcta tcgagcaagc ttgtaagagc agggtccaaa cgagcaccag tagaaggctc 62820
agagatcgcc tctgtagcat tttctttggt ctcccttgtg gttgggttag gaagggattc 62880
aagctcacac aggtggcctt gcagaagctc ctgtcggctc ttcatttggg tgatgtgtgc 62940
ctcaagtctc ccagtggtga tttcacaccc tgctcgttct ttctggaatt gctcttccat 63000
ctgtcgagac gcagcttgaa gagattggat atcattcttt gcaacggaca aatctccata 63060
caaagagaac gacagatatc caaaccctgc tgccattgcg gcagctacta cgagggcgta 63120
gtttttgagt ttagttagta tcatttccta tggtatcgtc ttctatggtt tccgaaatcg 63180
cttcctccac tttaccagca gttacaaaca ctgccgaaat tcttacaccg acagctacca 63240
gtgtggccac tgcggcgaat acgccgggag gaaggatagg ctcccaaaga tgaaccatgg 63300
cctccagagc cataaaagtt gcagagccta cagcagccca gaacttccag cttttcaata 63360
gcttcttcat taggaacctt cctagattta cttgaaggat ttgtaggctt ctgctagctt 63420
aacatcgtaa ttatttttct tgtaatcagg cccgttatac ctccgggcaa attccgccca 63480
gttcttggat ttcagagctt tgtggattgc aggattgatc ttgatgaagc gtacaaaagt 63540
gtttagttgc gatccttcgc tggcgtactg ggcattgaca aatgcctgaa cactcgcata 63600
acctagtgcc tcccaatgga atcccataat ctggaatagg ccccaagagg cactttgaag 63660
tgcgcagtct ctatccattt tgactgcttt atctagacgt tcgtgctccg cttgtccgcc 63720
gaggtatcct ccagctttag ggttgcaaac gtcgtttatt tcagggtcac gacctagctt 63780
ccttttgaga agcttgaaca tccagtgcct ttcgaataga atcttaggga cgccagaaag 63840
tagaaagccg ctcccacgac tctctacttt agtgacggcc tttacactgg caacatcgac 63900
tccgagatca tcggcagccg attggaagtc ttgctcagtt agagccattt ttgatactcc 63960
tgaattacag ggtggaagtg atagcaaacc agttgaacgg gtaagaccag ttgccccagt 64020
tgcagctaac tctaaacccg gtgttgctcc acatatccgt catgaaagcg cccgggccga 64080
tggagttgtt aaacgtcccc attacggtca cttgaacgtt caagattcgt gctggaacag 64140
ggagttggat attcgtcctt gtgtcatcag acagaccggg accagttccc cattgtagca 64200
gaaggccgtt tggtagtcga gcccatcctc tggaaatatc cgactgactg gactggaagt 64260
ccatagcagt tgtattcgca ggaagacccc atacatcgag cgtagccgct cccttcaacc 64320
ctaggttgtt tctagctgct tggacgttcg tgaggtcgga caggttagcc ccagacgaca 64380
ggaagtctcc tgtatttcta gtagaagctg tgcctagccc taggttggac cgagcggtag 64440
cagtgttagc caatccagca aggttgtctg ccttcatcat aaccgccgca gggtctgttg 64500
tagccatccc agtgaggcct aggttgactc tagcttgtgc cttgttaggt acatctgcta 64560
ggttctgagc gctcctcaag aaataggttt ccggctgggt agcactactt gtaattccaa 64620
gattcgatct tgccagcgcc ttgttaggca catctgccag attgctacct gccttcagga 64680
atgtagaaga aggcatagtc gctgtatccg acagtcctag attagctctt gcggcagtga 64740
cgttagacag accggaaagg ttgtctttct tcttcagata cacctgtgcg gccttgagcg 64800
gactcacagc cttggtatca ttggctccag cttctgtctc gatagtcgta gcaactgcca 64860
taaccccggc tacagtctcg gtcgcaggag gaacgagagt cgatagctgg ctcagcggga 64920
tcgcgtgagt tggctgtgtg gcagtggcga cgctgaaaac attagagccg ttcccgtgct 64980
tcatagcgta ccgggcgtcg ccttcagctt tactatacac acttaggttt tgtcttgcaa 65040
taactacgtt gccaatccca gagagggtgt tgtagtttgt tacatgccga tcaaggttgg 65100
cttgaacctt agctgcctct cccgcatcct caaacgccac tcgccaatat ccggctgtaa 65160
tgttcagagg gtcagttgga atcttgtcgg aatgggttct aaggcactta tagatgatcc 65220
cgttagcgcc ttgagtgtag ctcaggttac cctgatactc tgtcacgcta tcccattcag 65280
ggataccatg ttggttgacg tgagcgttaa acaggtcttg tttatattca atgaagtttg 65340
cagtctggta cggaggaagt tccgtaaccc agcctagccc tactttcaca gcacccgggt 65400
caaccttctg gccattttca gaccagatag cgttgattcc cgtaggcttc ataatgtttg 65460
gcatattatc ttacctcact ccttcggagc ttttggttta ctcttctcgg ctgggaagtt 65520
tttatgctca ggccacttac gtagtgcaga gcggtactta cgccaagcct tctcttttac 65580
ttcgtcttca tcgtcgatag caatttctac ttgacgatca gcccgggtta gctcattatc 65640
tctccacaga cgtgcatcta caacgaggtc tctatctgta acgtcctttg gttcggggat 65700
ttctccgtta tcgacagcac gggcgatggc gtcatatacc agtttggctc tatcgtcgta 65760
atcacaagcg tagaacggaa tccatcccca ctgaggatga tttacctcgc attcgaaccc 65820
cattttgtat tttacaacat ttctgaattc cactataatc tcctctaaaa ggagggccga 65880
agccctctat taagaaattc tctgccacag ggtaatctgg ttaccaccac ctgcaaacgc 65940
tcccatacat ctgtaagtgc ctgctgctgg tagtgcccct gctgtgtagc taatagtcga 66000
ataaggcttc aactggcttc cagctctagt tgtccccggt ccaccagacc ctccagattg 66060
atctcccgcc agaatatact gacctacagc gtcccaagcg gctccggctc ttgcgatatc 66120
gttaggaatt tctcccttct tggcatatac cgaatcaacc cagttggtca gagaaccata 66180
ggctccgtaa gcggctcctg tgatattccc tgctgtattg agcgtagcgc tcccggcgtt 66240
aacgttgcct gtcgcattca cgtttccaga ggcgtttagt gtggtagcat tgactcttcc 66300
agtaacagtc atgtccagac cggtaatcga cactgcggtg gctccgccag caactcggaa 66360
gttcatcgag ttgttagatt gggctcccca gataagaccg agttctgttc cgttcgctct 66420
ctggaacccg atgtgggcgt tgccagtgcc cgatgctcta ctgaagatgt tagagccgta 66480
cccgacgata gtgccatacg cattaatcgc aatagcgctg aatcctccgt ctgcgtctct 66540
ctgaacaaga gagttagcct gcgccgtagt tgtggctgga atctgagccg agttggacag 66600
accagtgatt ctagcggcag gaactgtacc atgagtcatg ttaccattag cgtcgaatcg 66660
gaacagccat ccgttagtgc taccgttgaa tcctccgata gccgtaggtt gtacggtcaa 66720
gtaggggcca ttgttcaagg tgctggggaa gacttgagta tcagagctac cttgagttag 66780
tctgtcgaga cccagacgag acctagcgtc tgtaagagtc gggcccagag agaactggtt 66840
tcctgtgtga aggataactt cgttgccaat ctgtacaact gggtttgtag cgcttctgaa 66900
tacaaactgg agaatgttgt catccactac agcaaggcta atccagtcgt tgtcagattt 66960
gttcctcagt gctagctgag tagtcttatc tgcactcgaa aggttcgctc cgttgatgtg 67020
aagacgtgca accgttcccg cataacttgt atctaggccc ggaagaacaa tgttttggct 67080
tccgtcaaac gccacaccgt tgattgtaac tggggttcta agcttgttag ctgtagttgc 67140
gtttccagac agggtagcag tgattacgcc agcgttgaag ttgccagcag catctctata 67200
gaccagagta tgtgccctgt tagctgggtc tgccgtaatg atagcagagt tagctagtcc 67260
taggaaattt cttgcgtttt catcattagc cgcgttgaca agtcctcttc cgaacgctgt 67320
gatgttgaag gtagccatgg aggtatccgt gttgaagtac ggaaccgtgt tggcagccct 67380
agtcacagtg gacaggttgg acagtacaac agagctttct tgagccgaaa tgtagcttct 67440
agctgcggag gcattagcta cattcaacat tccgcgacca taggccgtag agtcaaacac 67500
gctagctcct gtagcgctaa tgaagaatgg gattccaccc tctcttggtg tcaggcttcc 67560
aagtgcagag ctagatgtgg tgtaatctgc aaaagctcga acccagttgg cactaacttg 67620
tgcagggtcg cttcctgtat gcgtacgaac agccttgtaa acaaatccac ctctagtgca 67680
gaacgatttg ttagcaatgt actcttgtgt actagtccac tctgggatgc ccttctgtag 67740
caaatacgct aggttagtat cgtgaaggtt ccacttccag ttccaccatt ggcgaggaac 67800
cgactgaata ccccatccgc cttgctgctg cgcaggggtc ggggctacca tatccccgcc 67860
tgtggaccaa atattttcaa taggtagttg actgtaatca gccatttata actccttatg 67920
tcactaggtt ggtcagaatt ccaccaacag tgggatcgtt taggtctcca acgccttgtc 67980
ctccggggaa gccttctgta gcaaataccc ggccagcttg gaactcagtg tatgtgtagt 68040
taaccccgat agtcttagga agcaatgcac ctgcaccacc taggtcgaat agaagactac 68100
gctctacgtt cgttagaatc ttaccgatgc cgatacggac agcagcggga gcgtactcct 68160
ctaggaatac ttcaggaacc ccgaatacaa atttataagc ttcgataact tgctctgggg 68220
ttgagtttgt tctgttcttg atgatctttg ctttcaggat catccgatac tcttcgtcgc 68280
tcggctctct ggcgttacca gttggagcac cgaccgagta ccatggagca ccgaccgtag 68340
ggtccgacag gcttccgaaa gaacctgcac gctccgttcc agcgaatcca aaatagttga 68400
aaatatcttg gtacactaga ccgcgtggtc gccctacaat gtctccgata atgtccagtt 68460
gcttcccggt ggctgtgtcc agagaccgga gagtctggag gtcttccagt gcattctgga 68520
tatcaaactt gccagacaat agcacttgca ggaagcgatc aaagatcggc ttctctttaa 68580
actgttcagt gacccgttcc cgggccacct ccagataatc ttctctgtca aattggttga 68640
cagccatgga attctccttc tatcattaca ccgtagttac ggtgatatct gctgcggcga 68700
atgtagccac ttctttgaac ccgatttcaa tgttcgccat gccttgagtt tgtcctttct 68760
tggcaatcgt caggctgtta acttggaagc ccgggatttc catgattgga gcatagaatc 68820
gagaataaat tacatcgtct ccgatcttgt aattctcggt cccgtacgct acaatccttg 68880
gccgtaggat atcttcaatg ttctctggca tcgatcctgt gtttgttaca ctgatcttga 68940
tctcgattgg aacctcgaca gggcgctggt agttgactct atgggtatat cccagactgt 69000
ctaccacctg aacctctgta tctccaacag agccaatccc gaatggcttg ttcagccaaa 69060
tagactgtgc aacctctgta ggaataccgc caagaacgat tggaaggaac gagtgacccg 69120
ggactccatg aactgggtct gggttgtctg tatcgttttc gataatccta acatcctcga 69180
ctccttctac gttcatcagc ccgtcgatta gggactccag aatgttggtg gcttgtacga 69240
atttagagtt tcggaatctc tcgcgaagct catcgtcggt ctcacgcaga cggccagtga 69300
tagctggaac agggttgata acgctgtccc atccggcaat aggaatggag atcgtatcga 69360
tagctagagc ttgctgaggc acagggccaa taacgtcgtc aacagcgata ccaagctttc 69420
taaccttctg aatactcagg ttggaggctt cttcgaaagt cttggtgtta aatgggtcaa 69480
taggtactat gaaaagtata ccattctgtt cgtagcttgt caaggtattt ccgtgatttg 69540
tagctacgtc ggcggccagt cctgctagga tggactcgga tgttactgtg ccagtggcag 69600
gacttgtgta cttgatagac gttgtgttaa tgtcatcaac gtacatctta agctcgtacg 69660
tcaggtcgtt gcctactgtc tgagggaaga ttccaacacc agagcaagct ttaggagtga 69720
ggatcacgcc ttgtagtagg tggaacactc ggcctgtact gctggaaatc atcttcgcgg 69780
gaggcgagtt aataacggtg ttgatgttgc cctctagaac aacctgagcc cgggtcggac 69840
gtgcagcatg ccggctaatt gccgagaagg atactaggtt atccagagcc acgccagttg 69900
cggctgccgg gttgaaggag ttatacacca tctggataac ttcccagata gcttcgtccg 69960
atggagcaac tacgccgatc agacggccta gagcagagtt accagacgtg tcaacttcgt 70020
ctccagttga aactaggtcg gcgaagacct gagaggctac gtttctgtag tctgtcagga 70080
tttggtccaa gtctttaatc gttagacctt ctgctgtaat tccagccata ttaaactcct 70140
atcattctga aagtgatggg aagggattct gtattgtctg tggttctgac tgtgaactcc 70200
atggagtatt cacgtgtgtg agggtcgatc cccgactgcc aagatgtaat ctcaaggacg 70260
ccggggtctc tcataatctc agattggaat atcgcgtcaa cagcgggaag gctcctagac 70320
ttaccaagaa tcctctcgaa gtaggggatg ccgatcctat catccaagaa ccactcacct 70380
aggaatgtgt acagtttgat ctttagtcgc tgagcaacaa tatcaaccat tcgttgagtg 70440
actggagctt caccatttat aaaaaccaag tcatgtgtgt cggggttgac aaacaagtcc 70500
atatgatctc ctttaagctt gtgggacagc agtaacaccg ccgcccggca taacaccacc 70560
gtgtttgtga gtgtggaatg gaataccgtt gaatgtagaa gttccaccag tctgcacgta 70620
gttacctgta tgtgcaatgt tcccgttcca tgttgtatgg gctgcatcga cagtgagtga 70680
agcacacttg acgttgacat tttgagcatt aatgcttata gtcttgcggc tgttgattag 70740
aatatctcca ttggctttga tcctaacttc cgcctcttgt cccgtcatca ggttgtgggc 70800
aatagtaagg tctttggtat catgtggcca actatgtctt gcagggtcgt tcatcgacct 70860
agggaaggtg aacagcccgg gaatggcaat ggcatccgcc atgttgaact tacgaagatc 70920
gtttggagtg tgtggctgtc cagtggcaga gcctttaaac acgtccatag ttctttgaga 70980
gaatacacac aacaccgtat ccccaacatt caatgggaag ctgataagtg tgttagctgt 71040
tcccggcata atgacaggga cagatagaat ctctggttgc tcttccgatg tgccgtcttt 71100
gtacaggtcg ttgaccactg gtctaacatc gacagatagg cgtctaaaat cgtccagaac 71160
gcgcaatacc acacaaggta tggcagtgta ctgcttcgaa ccctctgtgg acgagtgcag 71220
gttcagaagc tcttgtaggc ctgttctcct cattggtcaa tatcctcttg gttaagatcg 71280
gaacaccagc attcagtgta ccattcgttg tctctgtatc cgcccgagat tcggacgttg 71340
ttgattctga aagtgcctga caatctgtcc gactctaccc ggacaagaac cccgggagtt 71400
agctcagggt tgagaaggca tttgaattga acgcccctcc gtctccgctg gtctttcttc 71460
tgtgctgttc cgctggcact ggcgtagaat gggatatcaa tcaggccggt ctctctgttt 71520
acgagaggag ccatttgaac actcttcgtt gttgggccgt taacgtctgt gacgtttaga 71580
acgtttccac tgatgttcca ttcaatattg ttggcttcac acacactgtt tagtgcgtct 71640
ttagccagtc cacgtagttg gtatccatac atgatcgggt tgttgcaatt gaggcctgta 71700
taagctcctc tgtccactcc cggcatttga agacgaattt cttcgataac atcctttaca 71760
gttttgcccg gagatactgt gcccttcagc ttggtctctg ttagtgctgt atatccttca 71820
cctagaataa gctgagtgac ataatcgtta ccactctttc tagtcgaagt ttcaaccacg 71880
ttgccatcaa gcacaacaga agttcccatt tccttataac caacttctag tcggcacgtc 71940
agatactcag tttcaagaat gttcagagtg gcaatagaca gattgtatat ctcaacagtc 72000
gccgagtttc cgctcttctt gttatctgaa cttttagaga tatcaaaccg gaactgaagt 72060
ccctctctgt tcaaacttcc gtcagggtgg aggtttgtaa tctctacacc ttgcccggat 72120
tcagtgttac cgagcgttag tttatacact ctgtcatatt gtttcactct acgtcatcct 72180
ccaagtcaac gttcacgtat agcagttcga aaaactgtgg tacaatgtca cttgcatcgt 72240
gcttatactg tacctgttct aggttgacag gcatcagtag gaagtagcca ttgaaaccta 72300
tatgttgcat ggcgtagtct gccatgatcg gatactgagg aacaaccgcc agtcctgtca 72360
ggatagggtt aagttcctcg tcgaatatat ccatgtgcca ctgccgagag cggctattcc 72420
agtagaattg aaggactctt gagattccct cgatagctac tgcgtatctg tacttccggt 72480
cttggtagag aggaagtgtg tcgatataag tcgtgctcat ttagtctacc tcgcaacctg 72540
agttctaatc tgagcctctt tcatttgact acgcttgaat gtaggagagt cttggctggt 72600
agcattgcta gccccggtat caccttcttt ggtgcctctc ttttctttct tagcggtctt 72660
tctacccttg ttaaccctca cctcgacata acgagtatct gtgaatgtca cagcctcgat 72720
tgtcatacga ggttgaaggc tgctacctgt ctcggcgttt tcagtgaaag acaagtcggt 72780
gaagatgcat ctttcgattt gatcccagat gataccgtct cggaagtcca gcaaactgaa 72840
gacctcagca ttacgctgca tgtcgatcag ttggcgctca atatcataag cgactttatc 72900
acccttttga gggaggacag ttgcagaagg aatctcagga ggaataaact gagtgataac 72960
ttctggaaag attctgctag ggtctagtct agaggttgag ataacagccg ggatggttac 73020
cggctgcgta ttcacgaact ctttgttgtt gatcttgtat gtctgggcct cgttgtcgtt 73080
gataacgggc ctgttcaagt tgaagtctgc gtctgatacg acggctgtaa gagtgaagac 73140
tgggttttct gtagtaatgt ggtctgtgat ttttcctccc gtttcgattg ggttggaact 73200
cacagagcca cggtactgtc ttccgaactc tgtaacagcg tcgaaccaga ttaggtctcc 73260
gttctctctt ttgatagcaa ttgtcattgg gattccttat aacttgccga agctctggag 73320
atttcttcct caagaacgtt actaacgatc tctcgtactc gctcttgcgc ttcagttgtc 73380
atgacgttac cctgagcatc gataccagaa acattgaggt tgaaactaat gtcaatggca 73440
ctcttcttga gggcaccctc catttcgttt ctaacttgac cacgttgcat catataccca 73500
aactggtctg gagtcaagga ctgggttgca cgggtatgag tctctgtctg gatcgcgtca 73560
atgttctgag ctactagcct gtcaccgatg aaggtggcct tgtcttttgc atcttccgga 73620
ttgaacatag cgtacatcgt gtcgatgttc gacattatag tacttctgtc tcctccggct 73680
tcagcaatct tagcgtccct gtacttgccg gctattgcga atctctctac aacactgttg 73740
aagaactcca tgatggcagc aagttctctc atcgtgctga ccagcatttc attgaactct 73800
agacctttga gcgcttcagc tagccccggg acaacagaga agataccgtc gagattgttc 73860
ttcagttcac tggattcact ggctagcttt tcgaatgtct cggcagcttg tacgttgttg 73920
ttcaagaagt ccccgaacac cgaatcaccg ccttccaaga agacaatgaa gtcttccagt 73980
gccaacgcac cccagctaat agcagagatc aacctgccca taggggtcag caaggctagg 74040
gtttgagccg tggtaagagt cagccctgtg ccggtcatgt tgaactgctt ggcaatgttc 74100
tcccactggg aaccaagctc ccctccaata ccgaccagag cgttgacagg cctcataaga 74160
gcttcgaacc cgccaccaag tgcagttacc agaggaagcg cttctttcat gccttgggca 74220
gcaatcttga agaagtcgct cattcctcgg tcgaatcctc cagccgcgaa ggcctcgata 74280
gtgcggttcc atccgaactg gaatcggccc tgctgagcgg ctgtaccttg catagccgcg 74340
tccagagcgc cacctttacg agcttcttcc gctagagttc tggcgaatgg gatcagagca 74400
tcactcttaa gcttcccgtc ttccatcgtt ttcatcagtt ccgcgatagt catgtcttga 74460
gacttggcca tcagagctac agcagcgggg aatctttcgg ccaactgacc tttcaattct 74520
tcggacataa tctgcccttt acccatcatc tgctcaaccg ctcggaaaga gcctttcatc 74580
gcttcgccgt caaggcccat cactcgacca tattcagcca tagactggaa gatgctgtca 74640
acttgttcct tgctgaaatc cgatgcctgt ccagcagcca tcatcttaac ataggagctg 74700
ccgagtgctc tgtcgtcgag acctaggcgt tgagagatat cacgtagggt agcttgcagt 74760
tcttgaccac cctgtactcc accaccgaca gcttgcatgg ccagacgttg accttgcagt 74820
tcttggttag cacggttaag ctgcataata gcgaacgcac caccaaggcc gggaataaac 74880
ccgcgtcccc acccggctag ctcgctacca atccccattc ccataccttg acggatatgg 74940
ccggcagtac ctctcgatcc acctgcacca cctcctcgga tggaagactc taggtcaatg 75000
cggattctca cttcgcgtct gatttgctca agaacatctt gaatctctct acgaagatgg 75060
gcacggtcta cagacagcct aatttgaggc gtagcgacag caagaccgtt tagcctaccc 75120
tgcacatccc ttagtgtgtt tcgtgcattc tggagcaatt tagagcggct aatgtctgca 75180
ttcagatgaa ttttaaaacg actctctgtg cgcttcttcc attcatacag cgtacggcta 75240
gcctgagcca agcccagttc aaccggtaga tcgaccttgg tctggccgag agccgtcttt 75300
acgaagtttt tctgatctcg gataccgcta aggtctagcc taacagactt caacctgatc 75360
ggcgttgcat tcagtctttc ttgaagcttc tcagagataa gacgaacagc ggcagcatct 75420
accgcgatgt tcttcaagac taccctttga gtggctgcct tggcaagctc ctctctcaga 75480
gtttttgtat ctagagttag cttaactcca aaccgcttgt tcgtcagctt ggagaattct 75540
cttagccggg cttcgacccc agcgagcttc ttgtcaaaag cagtgagagg ccgggaatcg 75600
actgtaaatt taagttcccc agttagctgg gcaatccttt ctgtaatcat ttcttatcag 75660
cctctgcgtg ttcttttgcc acttgagtga tagtatcgtg aacgtctata acctcaagga 75720
tgttgtacaa atcttctgta gtgaggactg tactcagttc cccatacgtc gttagcttga 75780
gagggtgagt gatagccaca taaatctccc aatgctgcga gaacttctgg tgggcctccc 75840
taaccctccg atcactctga actcgatcag tagtgactga agtctcggac tgcccatccc 75900
cggtctttat accgtcggtg cctctgcgaa accgattaga gaaaaaaccg agccgaagtt 75960
gaactcgatc acgtcttttg tgagtgcgta cagcaggtca tacttgccag cgaattcggt 76020
gtcgaaattg attgccatcg aacctttgaa aactgtcagc atcagattct ggatcaaatt 76080
ctcaacagga accttgtcga tgttatcgaa cagttctttc atggctccag agaagtcgtg 76140
gtcagcttga tacttggcaa gggatggacc cagcaagctg gttagctgct tcagtacgct 76200
gataccgcga gttgctggca gaaggttgat agtgtattct gtcccattgt ggatgacagt 76260
acgttggttt ccgaacataa atactcctta tttatctgag gtagctaaga gcgccttgaa 76320
cagttccaaa cacgtcgaag ccgacttgct ggtttccgcc taccgtgcca tcggtccagt 76380
ccagaacatc aatagtccag accctgttct cgattccatc ggtgaatgtt atgctagggt 76440
agccattgac ataagcgttg tctgtgctca tcatggtgct accgtgggta tccttgacag 76500
acaaggtgat tcgggcggat tgtgttcttc ggtcttgctc tactagcgag aagaatgcat 76560
cattagacac cgaagtctga agaacctcga ttctaatctg cgcagaaagg tctctattga 76620
agacccgggt gtggtgccct ctgattcctc gaaccattgt gtagggtctc gaaagccaag 76680
acatttcgaa agaaactaca ccagtgagag catacccaca aaggacaatt ttaacatcag 76740
atgggctata tgtcaatacc gtgttagcca ttagaagcct ccgaattgcg taagggctgg 76800
cagaagagag cttcccagca ggatagcttg ctcgaccaaa ctttgatcga cgtttccgcc 76860
aatgttcatg gcgacctcag agcaccctag aatccaagtg cgggtctcca gttgtccaga 76920
gaaagatacc ctaggtaggt cttccaccca tgctgtcaga gctaggaagc ttgtagaacc 76980
cttcgtgtcc ttaatcatca gtgggaattt tcccattcgg gttgcaatgt ccacgttgta 77040
aagcatcgaa aggatatcat tggtcggcga ggattgcatg attgtcaact ctactcgcca 77100
gccttggtct ttgttgtaga cccttgcgat ttcaccatcc atcgaaccgt gcttgatgaa 77160
aggcttaata tcttttacaa ttctgataaa ctctccctct gcgtaccctg tcacggaatg 77220
gataccagcg agagttacaa ccacgtcacg cggcgagtag gtggtaatca atttctatcc 77280
tccagaaaca acaaagggag ccacccgtta aggcagctcc ccgtcgatgt ggcataagcg 77340
ctatattaca gccagcgctg gtctagtgtg acacctaggt tacgaagagt atccgcatca 77400
cccgggctca gcttggcgtt accgccgatt agagtttgca ggttgtgtgc atgaataacc 77460
caatcgcgtg tctgcatgct gttcgagaag ttagaacccg gaacaacacc gacataggcg 77520
tcatcactga agtagtcgga acgaccagag ttgtctttaa cgtggattgt gaacaatccc 77580
tcagacgaac gacgggcgtc gtcgtagttg taaagagcag tcaggaagtc gttcgaaggc 77640
gaagtttgtt gaaggctaac agtgagggta gcagacttag aggcgttgta gacgcgagtc 77700
gatgtgttgt cggctccggt atacatcacg taagtgtcag cgtttctttc aatgttaacg 77760
atactgtctt cgctgaatcc gccgagaacc atgctctcac cagttgcagg gtgagtaata 77820
acaatggtaa catcagccgg agagaatgtc gctagacgtt gaacagccat ttagtaatct 77880
ccttatggga aggaccgtcc ttagacggtc acagttccac gaatgtgaac gaagtgaatg 77940
gcaccggcca gacgggcttc aaattcaatg ccttcaaaga tacgctgtgc tctcatgttc 78000
ggagacatgg atagaacatc aggtacgaag actttaggag ccggagcttc agccagacca 78060
ccaacacgaa ttccttcgtt aagctgggct ctgatttccg attccagaat tgttgcacca 78120
acggcgtcgt aagggatttt cttggagtta gccatacgga accacagacg ctcggtcatg 78180
cgggcttcca gccagtccac gaaaatcatt acgtcgatcc attcaccacc ggccatagcg 78240
ccaccgaatg tacggttgac accaccaacg cgctcgaacg tggtaacgtt cttgttcttc 78300
aggttggtag attcggttgg ggttagacgg taagcatcta cggcagctag agctttatgg 78360
gtccatgtgt tgctacccgg ttgctcttgc agttggtatc ctacccaagc acattcaggg 78420
aattgagcgt cagcgtttgg atggtaaatt agagccgtac gttggaaccc ggcagcaacc 78480
agacgagaag caatgtcatt ctctgcggaa gtcttggtgt tagcctgagc agtggctccg 78540
ataaacactt tcttggtgcc ctcgatatga gtagctactg ccatgatatc atcatcggcg 78600
tggctgtcaa tgctcagagc ataccattcg tcgttttctc cttgaactgc tgtgatagca 78660
gccggccaac cttcagtaga aggagcagca gccatagtca ggttagaaga aaccttcagg 78720
ctccagtctc cgttcgaagc gacagtcaga gttccgtctt cgttgtcagt tacggtaacg 78780
ccaacaacag gggtaacatc atatgcagct tttaggccag ttgcaattag ggtggcagtg 78840
tcgtcttgct ggctaacata gctgacaggt agtccgttaa cggtaaggac gtagctttgt 78900
ccttcctgaa caacactgac agagacggta gcagaaggaa cttgtctgcg tccgataact 78960
agactgcgag gcttgagagc ttgaccgaag tagcgaacag cggcaagata tgttggatcg 79020
gattctccga agtcttcagc gacacctttc aggctgttgt agactctggc acgttccgag 79080
aagtttgtgt gagaagcgat gaacagagga acgttgaagt ttgtctgtgc gacagcagca 79140
gtctcacggc tgatctgaat gtcaattaca tcagtaagaa cagtcattcc tgtctcctat 79200
tagtgttcga agtttatgtc gatgtgggac tcaataatgt gacccgggcg accagcgtcg 79260
taataaacgc cgtgaatatc agccccagca gcaaactctt gagttgtggt ttctataaca 79320
gagaatccaa attcacatgt aacttgatag aacatgtaca tatctgtttc tcttagcata 79380
ggaactcgtc ttgtgctagg gagtttgaaa agggatagat taaactttct ttgggcggcg 79440
tgtcctgctg gagtacgcat tgcccgagta atcgccattg ccatgtcaga gacacttgct 79500
gtctctccat acttgccaat cagttcgaat acgactttgg ctgcgaagtc ctgttgaact 79560
tgaatagttc catgcgaagg actaatggga tcgctcatat tggagacatt ttcccttccc 79620
agttcatcca gcctcttaat gtcgatagcc agataagggg tttgcggttc ctgattattc 79680
gtgtaagctt gaattacacg ccagtcgggg aacaactctt tgaccataag atagagggag 79740
ttttctagct ctgtgtggat catgctgtgc tattcctctc tactctcata caaattgctt 79800
tgtaatggtt taggattccc atcgaatagt tgatgacttt cataacttca tacaactctc 79860
cttcccacat gaatctatcg gcactccacc ctcccgggcc ttcctttaac tgtcgaatct 79920
cttcgcctct ggagtacacc ttgaagcaag ccttgcttct gtcggcctct ggaaggatac 79980
gagtgtctgt cgatttttcg ataggttgaa cgttagctgt gattgttact actgtctcaa 80040
ctggaggggt aggtcttcca aaaccgtcgt ctccgggctg ccctcttcta atcaaatcta 80100
gctttacgaa gttggtcatg gagaaggtag gatagagcat tattgcctcc gatggatttg 80160
gaacttgaca gactcaagca tctttccagt atggaataga ggatcgttaa atcctttgta 80220
agctgcccat gccggactgt tagagcccgg atagtcgtca atgttaactt gcatctgctc 80280
tgcaaccatt ttacccaatt tcttcagaag agtattcgtc tgccttccat cgcgtaggac 80340
gttctcgaac gtggacttca ttagtcttgc gtagtggaat tgagatgtga attcttcaaa 80400
ggttggagcc atgaatggcc gggttgggtt gcgagtagta ccgaattcgt tgtaagctgc 80460
aacttgtgca actggtaggt ttccattttc agaaccatat cggtcctcgg ggaagaaacc 80520
aacttctacc gttgttccgt ctagagcctc caccctctta atcaaggatg tgaggcgacg 80580
gcgatctgtt ttaaaactga agcttgccaa gataatcaca ccccatgcta aggtcttgag 80640
gaccggggtt gcactggttg taaagcttct cccgtcttgc aaaaccgatg tagaattccc 80700
gtgttacgtt gtcggggtct aggtcattag ccagcatatc cgatttggag ataccgccag 80760
catatggcac ggccaaagaa attgtaatat tcgggtcttt gagaatgagc tgaagagctt 80820
tcaagtagtt gttgaaatac tcagcaccat atacttcgat atcacctgtg cgttctctca 80880
ttccccttgt caggcggaag agcatcatac gcataagttc aagtgcggct cgtctttcgt 80940
tgccttcgtt cttgtccaac acccattgat aggtttcgtc ggaaagcatc ggaggctccc 81000
acacgtcccc tacaatctct cgtagacggt cgatagggtt gtttgctgga tcaccagtgt 81060
aagccatttt tggtaccctc ctacgacgaa agggaggccg aagcctccct gaagtcttta 81120
cgcattcaca gtgccgcgaa cgatcatctg tggacgacgc agaacgtgga tgaagttgga 81180
ctcggtttcg atcaggatcg aacgacggtc ttcagccata cgctcggtag cgtacagttc 81240
tttacccaga gttcctaggt catcgaaagt gctgcccggt ccgaagtaac gagcgaacag 81300
gtcaccgctg tcggttggga tgaagtaagc atcgccagcc gggatcagac ggttgccgta 81360
cgggtcaacg ccacggtatt cgatgaagtg aatgtttccg aagtagaact cacggaagcg 81420
aaggtccaga ccgcgagcgg ttagacgttc acgccagtat tgaggactag cttggtaagc 81480
tttgtagaac tctttgatgc tcggatggtt aatcagggca gtgaagaact caggagagca 81540
cagggcaacg aagtcaccac ggatcagacc gtcttggctg ttgtcttgca tatggacgaa 81600
gatttcttcg gtcttgccca taacgtcagc ggtagggttg gtcaggtcga agccgacaac 81660
cttacgagtt ttacccatca ggtcgtacca gtcgtactcg atggttccgg ctggggcgta 81720
gctcttacct gttacgatag cgctaacgga agcaacttca gcggtgttag cccagttgcg 81780
acggatggtt tccagcttac gagcacgaac ctcgttcaga gtttcttcgc ggtcagcacc 81840
gaaagcgcgc ttaccttgaa tatcttctgg agtgatggag tcgtcttggt tgaagtgcgg 81900
aatcgcgaag gcacgcagtt gacgacgttg gtcgctagca acttggtgac gagttccacg 81960
gatggcatcc ttaaccagac cgtagcgagt ttcgatctct tcgaactgga cagtgttctg 82020
agtcacacct tcacttgtga agacgcccat gttctcgacg agggtccaag tgttcgggat 82080
gataagcagg ttttcagtct ggtcggcaat gtagaactga ccatcgttta ggtaggaacg 82140
ggtgttagcc atttttaaag ttactcctag tatttattta ctggaacgac cctcgcttac 82200
gcgaaggtaa cgttctcgaa tgcgccttcg atctggtcaa cggcgaagat gttcttggct 82260
ttgaaagctt caaagacggt agctttatca gcgtcgtcaa cgtcagcacc gaagataaca 82320
gccttggagc cgatcttggc aaaaccgcga gcaaggacga cagcgtggct catctgagcg 82380
ttggcatagt cgtgcaggtc ggtaattagg atgtaggtag cgtcggcgat agtagcctta 82440
gttaccagct tgccagtgct gtctaggaca gaaccagttg ttttaacatc aatgtcagcg 82500
acaggcagag tttcgcgagt gtagaacagg ccacggtggc ccatttgttc ttctttcagc 82560
cagttgctaa gacgctgatg atcttttgcg ataatgtttt gagccatttg gaattcctct 82620
agattggcta gatattactt ggagccgtgg cgagctttca ggatagcggc aacaccggcg 82680
acaacagagt cgtcttcttc cccagcgccc gatacacctt tctcgacaag catgtcattt 82740
tggtcagcag cagctttaag aatgcggagc gttcctactg tttgtgcaaa agcttgatcg 82800
tccagaactt ccagagactt gaaagtatct tctacttggt cagccgggag gacttcagcc 82860
agttgctctt tacgggcttt agcgatagca gcggttttct ctgcctcagc agccttgaca 82920
atttcacgaa gagctgtaag ctctacatta gcggcgtcta gttgcttctg aacttctgca 82980
acagcagcga caccagcagc tttttcaact gcaatagcgt cagctagagc ttctaggtgg 83040
gccgatttca ggatagtttc catgttggta atctccttgg atggattttc attggattgc 83100
tcggagacta cctccagagc cttttcaagc agttcctgat cctgtttaag ggaaagggtc 83160
tgctcagggg taagctcgga gagagcttga ttcaggtcag ccgatttata aaccgactta 83220
agaatggaca tagactcgac ccgctggtcg atccagtctt tgtgatctag ttctggatac 83280
tccgttctgc catatcccat ggcaacagag agaacttcgg catcttcata atacatcccg 83340
aagaacttac gaaggaactc agggaactgc attgtgactg taacttcagc agctttatcg 83400
ataagctctt cagttacgcc attggtagct tttgtgatta gagtagtgtg cccgttggct 83460
gcaccacctt ggagcttact gaccagagcg acatgagcac cttcgtgttc aaacttgata 83520
tcggtcagtt tacgttttgc ttttacggtc attcgatatc ctcgactgta gctctggcac 83580
cgatagagac gcctgtgaat tcaccagatt taacgccttc ccatagtgca tcggctactt 83640
cagagccttc agggaagtgc caccactgga gccatgtacc tttcttgatc tctcttccgt 83700
cgtcagttgt gaatgcggat ggagcgatga aggactggac aatctcagct tcctgtgttt 83760
caatcttgtg gaagatatta gctacgttgc aatgagtatt gaagttgatg caagctttct 83820
ctacttcctc ttcggagtag gtgtctgcgt gtaggtcagt cgtggagtcg ccttcttggg 83880
gctcaagaac gacaaacaaa gccatcctct tttcattgtc tagggacttt gtgacctcta 83940
cagccggggc ttcttccgcc ttggcgggag ccccgaaagc attgctgaga gcttcaataa 84000
cactttcaag agtcagcatt ctgtctcctt atgcgttatt tgcagcggcg ttgtctctac 84060
cggctgcgga tgttgatgca ccattaccta ctccagcttt acctgcttga gactgaccac 84120
ccatgatatt atccacatca acaggctcgt cgtctggtcg aggcttaatc ttgagagctt 84180
tgcgaatctt gttgtaaacc tcacggtcac gttcaagacc accgacagaa ccgattctct 84240
gaatcccttt cgagaactct tcaagatcga tttcatctag ctcaccgtat tgcagcttcg 84300
gcaagtctgg actataatca ccgttcagtg cgaacagcgt aggaatgagt ttattatcta 84360
gtacgtcttt gatttcgcgg agccgcgatt caatcgccat agccatgatg ttagtcttgg 84420
caccagctag agagtaagag ccaacttggt cctgacccat cttcagcata tcggcaaaca 84480
aggcttgcag aattttgttg tcccagcgac gaatgatggc atctgtatca tacatcttac 84540
tgccctgcga agaggtcagt tcgaatttga acagaggttg tcggctttct gggtcaaacg 84600
cttgaggaag gataagtcca gattgttcat tcatttgaat gttacgaatt actcgcttgt 84660
agtattcgta gactgccctc tcagcttctg tagcatcttc ggacatatat cgaggaggca 84720
gatagagcgt aggcataccg ttcatatcgc gtgttacgcc aacggactct tgctcttcaa 84780
tctctactcg atatctccaa gcgttatagc acgcacggag agggctgtta ccttcaggac 84840
tgtcacgttt agcatcgacc cggaaaagaa gcatcttctt agctggaatc tctacagttc 84900
cgtccttatt acctaggttg acgtaacggt cgccattgac aacattggca agactttgct 84960
cacaccctag gaagtttcta ccgtcttcag agtacaacca tttggtgata gtagactgag 85020
agcggattgg caacttttta ataccgaatt tgttgtcatt gtacttcgat acagggtatc 85080
cttcacgtcg cttaaacacc atttcatgta cggagaatcc gtaggtgtag aagctagtga 85140
cctctttgat gaaggagtac caactgtgtt ccatgtcatg catgcactct ttcaggaatt 85200
taccccgggc cttcatagcc tcgtcggggt caactcctag atcaacttcc cagtcaaccc 85260
tgctcatcat catttcgaag agatcgagtg ctgatttgat ggtagggtct tccgccatca 85320
tttggaatgt tctgcatgcc ctagggaacc gaagttctgg tcggcgttct tccagaatag 85380
taccattgac ttgtttaagg cctgtactac caatctcgcc taggcgaagt cgcgggatac 85440
ccggtgctgt cttttcagtc tcggccatcg ccgcctccta ttaatttagt ttgaatttgt 85500
tggattgagt catatcaggc ggtaggaaat cagggataat caagctgcca agggatagag 85560
cattaaatgc atctgatgta gcgtcaactt ggtcatcttt gatagcctta cttccgtcga 85620
atcgctcaag ttcatcgaag taatcttcgt tccaatctcc cgcaacgact tgcactgatt 85680
tagtttcagc tacagatacg aacggggcga atcgagtaac tttcgatttg ttagttgtct 85740
tcggtctggc gtggaatcca tgatccgcta ggtctctaat tagctgggca gcataagctt 85800
taccagcagc ccccgggtca catgggattt gaatttcaac gtcttgaccg tcttgcttgg 85860
ctgtttctag aatgaggtcg aaaacacctc cgaacaacct acgctctctg acaacatcct 85920
cgacagtgaa caagccttct tttgtacggc tcatcagcac gccggctgtc cagtcggggt 85980
ttctgttggt ttcggattca atgctaccgc tgatgtccca tgcacgtacc cttttaagca 86040
cccgtagagg cggatgggga acgatttggc accagtccct tgtgaagtat ccagctccct 86100
cttctctagc gtgccagcta ccgtctagga gcttttcacg ttctacccta ggaagggctt 86160
caaggttagc tacatactca gggtcgttga gcatcaagat tgggttatcg aaacagttag 86220
ccgcgatgaa tttgaagctg ataggcttca cctgatcttc atcgtcaggc gctagactgg 86280
gattaccatg aagggcaatg cactcttcct tggtgtctcc ccagaacatc ttaccgcctt 86340
tgcgtacaaa ccagcgagtt acgccggagc gctcagggat cgggattcct gtgtcagggt 86400
ctagccacca ttcaatccat ttacgaagga acgaattgta atcagggtta catgtaatct 86460
tcatgtgcgg aacaaccgag catttcgggt tacgcatacg agagataagg tatgtcacca 86520
tgccttctgt gaactgctga ccttcgtcga ctaggaactt actaacctcc caaccttgga 86580
aggagtcttt gtcagattct tgttcgaagt gtcgaaggaa aataactgca ccagaagaga 86640
acacaaactt gccgtctttg tctttccact tgactttagg gtcaacgagc ttgaataggt 86700
ctgttgcttt ttcaagcaga ccgcccgggc ctttaatctg aggagtagta cgacgagtca 86760
ttacacctcg gaagttagga tcatggatat atttcatgaa gtccattaca ccgagataag 86820
acttaccagc accagcagca ccaccgaata cagtgatctt tgcatcgctg tctgtgaact 86880
ctttctgttt gaaagatttt ggaccaataa catgagggct gagatcgaaa ttctctctgg 86940
cctctttaag cgctagcgcg attggtccga gttttttatc aggaacctta gtgttttcca 87000
tgctacaacc ttatagagtg tgaaccctat cgggagcttg cgggttcttt gatagtgaat 87060
tggtgcccca ttcctgactt gaacagaatt cgccgggtta caaagccggt gcatcaccat 87120
aaatgcttaa agggcaggga atagagcctg tgagacggga aaggaggagg aaaccgttca 87180
gcacaggcga ggaggaaaga gtgtcatgtt gtggaatcga accacctatc gctctgctgc 87240
gagagcccgc tattctcgct ttactggctt acgatccaga gagcctaaaa gcgtcaacgc 87300
gatataccat tgcacgacat tggttggagg cggggactcg aaccccaaca atcgggtcac 87360
aaccgaaagg cagcatccgg gctacacaat ctccacacga ataatgccaa gccggctgaa 87420
agcacatacg gaggaatgtt ccggcttgga cttgattgga gcagttaccg gggatcgaac 87480
ccggaacacc agattgaaag tctggcgatt taaccaatta atctataact gctttatttg 87540
gtgcatctgc ttggtaacga tccaagtcct ctggaatttc aacccagcgc ttctacctag 87600
ttagcttcag acgcgtatac gtagaactca catcttctcc cgggcgttac tccgggttac 87660
tgaggtctac gacttttggt gcgggcttag ggattcgaac cctagtctct tgggtggaag 87720
ccaagaataa taaccagact atacgaaacc cgctttaaat tggtcacccc ggaaggattt 87780
gaaccttcga ccttcggatt ccaaatccga cactctaacc agactgagct acgaggagat 87840
aataaggccc gtatcgggcg ttcgactctg cttctaaagc gaagagtctc ttttggaaat 87900
tggctccgta cctaggaatc gaacctagcg cgtttcggtt aacagccgag ccccatcgcc 87960
ttgatgggcc gtacggaata aaactctgga gcctcgtatc ggatttgaac cgatgtatgg 88020
cagatttgca atctgctgcc tgatcctctc ggccaacgag ggcaataaac taggaggttt 88080
ttaacccggg cctctccggg agatactgga tcacctccta tggcttacag catctaattc 88140
aaatctagga gctataacgg gcccgagccg gacgatttat actggctgcc agtagcttat 88200
cacagctact caattacccg tgccagctta ggcgcttccc cttaccgaga taggtagcga 88260
cactgaaccc accacttagg gttcctcgga gtgcaatttg gaagcgagag gtggaatcga 88320
accacttgta ttcggttatg agccgagtga cagcgccagc cagtcttaac gctctcgcta 88380
tttggtggtc gaaggaagga attgaacctc tagcctatgg cgtccgggtt acagccggat 88440
gatagtagcc atctatctct acaacgacct tgttggcgac ccatgtcgga ttcgaaccga 88500
cgaccttgcc ctagacaggg gcttgctcta gtccagactg agctaatggg cctttaaatt 88560
ctggtagcca gagaaggact tgaaccttca accacccgat tatcggtcgg gtgctctacc 88620
aattgagcta cccggctaca ttgtttgtta ctctatgtac ctatattatc atgaatttct 88680
tgtttgtcaa ccccttcatg ataactttta tagcgccggg gaggtcggaa agcagcgcta 88740
ccttcgtcac gtcccctagc tctctcacta tttggccgcc atagaccggc cttaagtgac 88800
cgggtaggga ctcgaaccct aaccaaatag caaaagagat tcttaatttg gtggccccgg 88860
agggacttga accctcaatc ctctcggcgt cagtttctaa gactgatgtg tataccaatt 88920
ccaccacagg gccttaattt ggcagcgagt aaaggagtcg aaccctcacc gttcatcaca 88980
gcggcctagt tttctagact agttcccgac ctactcaggg ctactcgcta agtatttctt 89040
catctgataa gtgtattaaa acatgaaaaa ttcagtttgt caacacttaa aatcacttat 89100
tttggcagtg agcagtggtc tcgatccaca tccgcgttag cagacgtact ccttagcagg 89160
gagcctcaga acctatctga tttactcact aaatttggtt gaacgggtga gactcgaact 89220
caccgccatc cgacttaaaa agacatgtgt gcagacgcta aactacaatc tcatatcctt 89280
caataccagc atgcagcttc ctgtggcagt ttgaacacag gatggcacac tttctaatct 89340
cttctttcag cctgagtctg ctgtacgaaa caagcttcac gacctcggta tctttttgtg 89400
tttggtcgat atggtgaaag tctaaagcaa caggctcatt ctctccacaa aacctacaac 89460
caaacattct tctcaacctt cccaagaact ttctgttgcg tgcaatagta tccttgttcg 89520
attgtagtat cttggaccta cgtttaggta gacgcttata cgtgtctgca ttgatggcag 89580
aataacattc cttacagtat gtctgtaaac catccttctt tcttttgttt ctggagaagt 89640
cttcaactgg cttctcgatt gtgcagcgag ggcacagctt catatctcct ccttaaaagc 89700
ccgatgccta tccactttag cttccgtcca tcttaaattt ggcgctcgta ctaggactcg 89760
aacctagacc ttacggctta gaagaccgtt gtgcatccat tacactatac gagcaatttg 89820
tgtcactata caggagcctt actctcctgt caacaccaat tggagagaat accgagtctc 89880
taaagaacat cggtcgctag cagcatagcc tagctctccg gctgcgaact tggtcccaga 89940
aggattcaag ttccatataa tttggcgggc cgactccgat tcgaacagag aatacgagga 90000
tttggaatcc agtaggttgc caattacgct caccgaccct taattctgga ggacacttat 90060
tactcgctgt cctctgagcg agacacattc cttgtcgggt gaatgttctt ttactacatt 90120
tggcagccac tgatgggatc gaaccatcgt ctccagattc aaagtctggc gtcctaccat 90180
tgaacgaagc ggcaattaat caaaacgcga gaacgactcc gggagtcttg tcctcttgag 90240
attcctcatt atctcctgat tctgttcctt ttgcaagctc ttttttgagt gcaatgtcag 90300
ctttgtagta agcgacgaac atgtcagaaa gctctttctt gagcttgatt ctcatctgta 90360
gagagacctc tgggttgtca gattgctctt ggaggaatct gatgttctct gcaaagctct 90420
tctttagaag tctaagccct gctttcaggt ctgtctgaat gataccccgg gcttctagct 90480
cggcattcgt agggcgaccg cccttgtttc tttcttgagt catattaaat cctctgaagc 90540
tttgtaagcc ccatatactc cgattatagc acgaaatctc ctgttgtcaa gcccttaaag 90600
aattattcgc accgtaatct actatttata catattgtca gagtacgcgc actctatata 90660
caatgtcagt ccccggtagg gagaggagcg gtaacccctt cgcttggata ccaaccacca 90720
aaatactcat gatccagtgt aggattgaga gcaaacctac ggcaagagga gcagagatcg 90780
tcctccttac cagtatgttt attgatgact ggagtctcaa acgagagttc gctatcacag 90840
cacttacatc tcatagaaat tccttatggg ttgattgatg gattatctgt ggtgggtatt 90900
ctgtgtatgt gtatcaggag aacaccccat gcagatagat tcggctaggg aaggatagag 90960
tcgtcccaag gctcgccata cagaatttaa tctgcctaag acgtgccttg gctctatcca 91020
attcctctta ctcatgcaga tgagttcggt gaggggcagc atgtaggctg catcggattg 91080
ctgtcattcc gctttcgcgt cgtctccacc ctttaacagc attcgagttc aggttgccct 91140
tgtggattcc tcttcatagg cgctaggcgt gggccggtga gtgctttcag acatatctct 91200
cccacgctga atattgataa taccacagat ttcctctatg tcaagcctct accagaacaa 91260
aatgggttga cattcctgag aaacgtggta ttattgtaaa acactaaccc gcaaggagta 91320
aaaagaatat gcaagttatc aatatcgtag aatacaaccc tatcgtctgg cttcgctcga 91380
tttacgccca catgaaggaa ggtttccgag tcgtccgctc catcgaaggc taccctgtgt 91440
acggcaccct ctgtcaagta cagatgttcc gtcaagaggg cgtttctgag gaaatcgaaa 91500
aagagcttga cggcaatgaa gtcattgtgc aagaatggga cgttttcagc cttctgatgg 91560
gcatgcaaaa tgctttcgat caaggtttcc gcattgatct cgaagaccct catttcgcca 91620
atatgaattt cgctctcaag tctgtcaaga tggtccgaga aggcgcagag aagcagactg 91680
aggaaaagtc tacagaagta gctaaggaag ttcctgtaga ggaagtccaa gagactgaat 91740
cggaagataa accaaaaacc cgccgtcgtc gtactacaaa ggagaaaagt gaatgaagtt 91800
taacaaagca caacgtgagt ttttcaaaga ctacgcaatt cagactgtaa agtctaccct 91860
gatgcaggtt gctgcacaga atggcctaga gatcaatcag gaagctctgg atcgcgctat 91920
ggaagtcgta aacctcgaac ctctggcgga agcagccggc gctgcgttct tggaacgagt 91980
agactttaaa accctcgtta aggttgacaa gtttgtacgc actgaagagt tcctgagtgt 92040
agctcaggct ggtgcagaag ttggtgaagc tgtcagcgga gagctgagca caatcctcct 92100
tccattcttt gcaatgctgg gctctggcca gaaagaaagc gaggagtgat tcagtaataa 92160
gggccctttc gggcccat 92178

Claims (5)

1.一株高裂解性铜绿假单胞菌噬菌体(Pseudomonas Aeruginosa phage)RDP-PA-20001，其特征在于，透过电镜观察，该噬菌体头部为廿面体，且立体对称，头部长60.865nm，噬菌体尾部长度为183.254nm,为有尾噬菌体目(Caudovirales)长尾噬菌体科(Siphoviridae)；基因全长92178bp，不具有毒力基因和溶原基因，保藏编号CGMCCNo.21410。
2.如权利要求1所述的高裂解性铜绿假单胞菌噬菌体(Pseudomonas Aeruginosaphage)RDP-PA-20001在制备抗铜绿假单胞菌制剂中的应用。
3.一种用于防治铜绿假单胞菌的杀菌组合物，其特征在于，包括有效量的如权利要求1所述的铜绿假单胞菌噬菌体(Pseudomonas Aeruginosa phage)RDP-PA-20001；该杀菌组合物中加入噬菌体RDP-PA-20001后，1小时内有明显的裂解效果。
4.如权利要求3所述的用于防治铜绿假单胞菌的杀菌组合物，其特征在于，还包括用于抗铜绿假单胞菌的抗生素。
5.一种清洁剂或消毒剂，其特征在于，包括如权利要求1所述的铜绿假单胞菌噬菌体(Pseudomonas Aeruginosa phage)RDP-PA-20001。
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