CN112358348A - Nutritional agent for improving oil yield of oil peony - Google Patents
Nutritional agent for improving oil yield of oil peony Download PDFInfo
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- CN112358348A CN112358348A CN202011246537.8A CN202011246537A CN112358348A CN 112358348 A CN112358348 A CN 112358348A CN 202011246537 A CN202011246537 A CN 202011246537A CN 112358348 A CN112358348 A CN 112358348A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a nutritional agent for improving the oil yield of oil peonies, which comprises the following raw materials in parts by weight: 5 parts of fulvic acid, 2 parts of monopotassium phosphate, 0.1 part of ammonium dihydrogen phosphate, 0.2 part of gibberellin, 5 parts of bitter melon seed extract, 0.1 part of chitosanase, 0.1 part of silicate bacteria, 20 parts of vermiculite powder, 6 parts of composite extracting solution and 3 parts of modifying agent; wherein the modifying agent comprises the following raw materials in parts by weight in a mass ratio of 1:0.5:0.05:0.2: 1: sorbitol, sodium dodecylbenzene sulfonate, radix Sophorae Flavescentis extract, Kochiae fructus extract, and semen glycines powder. According to the invention, the compound extracting solution is added, so that the absorption of phosphorus by oil peonies can be effectively promoted, and the contact angle of the nutritional agent and the leaf surfaces of the oil peonies can be increased by adding the modifying agent, so that the oil yield of the oil peonies is improved.
Description
Technical Field
The invention relates to the technical field of peony planting. More specifically, the invention relates to a nutritional agent for improving the oil yield of oil peonies.
Background
The peony for oil belongs to Ranunculaceae, Paeonia and Paeonia, and is a peony variety with more seeds, large fruit pods, full seeds, more oil content and stable yield. The peony for oil is mostly distributed in Shaanxi province, Anhui province, Shandong province and other places, is drought-resistant, barren-resistant, high-cold-resistant and easy to plant, and can be processed into high-grade edible oil, high-grade cosmetics, high-grade daily necessities and the like, so that the peony for oil is vigorously developed, and has important significance for promoting the production of grain and oil in China and ensuring the safety of the grain and oil in China. At present, the oil yield of the oil peony is low.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide the nutrient for improving the oil yield of the oil peony, which can effectively promote the absorption of phosphorus by the oil peony by adding the composite extracting solution, and can increase the contact angle between the nutrient and the leaf surface of the oil peony by adding the modifier, thereby improving the oil yield of the oil peony.
To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a nutritional agent for increasing oil yield of peony for oil, comprising the following raw materials in parts by weight: 5 parts of fulvic acid, 2 parts of monopotassium phosphate, 0.1 part of ammonium dihydrogen phosphate, 0.2 part of gibberellin, 5 parts of bitter melon seed extract, 0.1 part of chitosanase, 0.1 part of silicate bacteria, 20 parts of vermiculite powder, 6 parts of composite extracting solution and 3 parts of modifying agent;
wherein the modifying agent comprises the following raw materials in parts by weight in a mass ratio of 1:0.5:0.05:0.2: 1: sorbitol, sodium dodecylbenzene sulfonate, radix Sophorae Flavescentis extract, Kochiae fructus extract, and semen glycines powder;
the preparation method of the composite extracting solution comprises the following steps:
a1, mixing herba Leonuri, herba Menthae, rhizoma Alpiniae Officinarum, Capsici fructus branch and sweet potato leaf at a weight ratio of 1:4:1:10:30, cleaning with 75% ethanol solution for 3 times, cleaning with water for 2 times, freeze drying, pulverizing to obtain pulverized material, adding CO into the pulverized material2Adding 10 times of water, 2 times of acetone, 0.1 times of ethylenediamine and 0.1 times of sodium bicarbonate into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 95 ℃, extracting for 50-55 min, filtering and concentrating to obtain a first concentrate;
a2, adding dichloromethane in an amount which is 2 times of the total mass of the first concentrate, and then dropwise adding 1M hydrochloric acid solution until the first concentrate is completely dissolved to obtain a first mixed solution;
a3, purifying the first mixed solution by silica gel column chromatography, wherein an elution system is a petroleum ether-ethyl acetate system, collecting an eluate with a volume ratio of petroleum ether to ethyl acetate of 1: 0.9-1.5, and concentrating to obtain a second concentrate;
and A4, adding water in an amount which is 3 times of the total mass of the second concentrate, and then adjusting the pH value to 7-8 by using a 4M potassium hydroxide aqueous solution to obtain a composite extracting solution.
Preferably, the preparation method of the balsam pear seed extract comprises the following steps: mixing cleaned semen Momordicae Charantiae with 3 times of water, grinding at 70 deg.C for 10min to obtain slurry, adding 0.01 times of cellulase, hydrolyzing for 1.5h, heating to 100 deg.C to inactivate enzyme, adding 1 times of starch, stirring, gelatinizing at 70 deg.C for 20min, drying at 100 deg.C, and pulverizing to obtain semen Momordicae Charantiae extract.
Preferably, the particle fineness of the vermiculite powder is 10-20 μm.
Preferably, the preparation method of the extract of the sophora flavescens roots comprises the following steps:
b1, pulverizing radix Sophorae Flavescentis, and adding CO2Adding 10 times of water, 5 times of N, N-dimethylformamide, 0.1 times of diethylenetriamine pentamethylene phosphonic acid and 0.01 times of phosphoric acid into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 98 ℃, extracting for 60-75 min, filtering and concentrating to obtain a crude extract of the roots of the sophora flavescens;
and B2, adding methanol with the amount being 2 times of the total mass of the crude extract of the sophora flavescens roots into the crude extract of the sophora flavescens roots and dissolving the mixture to obtain a first standby solution, taking petroleum ether with the amount being 80 times of the total mass of the crude extract of the sophora flavescens roots, then dropwise adding the first standby solution into the petroleum ether under the stirring condition, standing for 5 hours after dropwise adding, and filtering to obtain the sophora flavescens roots extract.
Preferably, the preparation method of the kochia scoparia extract comprises the following steps:
pulverizing Kochiae fructus, adding CO2And (3) adding water which is 10 times of that of the kochia scoparia into a supercritical extractor, extracting for 90-93 min at the extraction pressure of 20-22 MPa and the temperature of 75 ℃, filtering and concentrating to obtain the kochia scoparia extract.
Preferably, the particle fineness of the soybean powder is 10-20 μm.
The invention at least comprises the following beneficial effects:
firstly, the compound extracting solution is added to effectively promote the phosphorus absorption of the oil peony, and the modifying agent is added to increase the contact angle between the nutritional agent and the leaf surface of the oil peony, so that the oil yield of the oil peony is improved.
Secondly, the complex action of fulvic acid and phosphorus can be promoted by adding the compound extracting solution into the nutritional agent, the absorption of plants to phosphorus is accelerated, and the oil yield of the oil peonies is further improved; 2 times of acetone, 0.1 times of ethylenediamine and 0.1 times of sodium bicarbonate are added in the extraction process of the motherwort, the mint, the galangal, the pepper branches and the sweet potato leaves, and the extraction method can extract various organic matters, and the synergistic effect of the various organic matters is more favorable for promoting the complexing effect of fulvic acid and phosphorus; the first concentrate is treated by column chromatography to remove pigment and impurities; adjusting the pH value to 7-8 by using a 4M potassium hydroxide aqueous solution, which is favorable for improving the water solubility of the effective components and is more favorable for the absorption of the effective components by the oil peony roots; the addition of the balsam pear seed extract is beneficial to improving the dispersibility of the fulvic acid, delaying the precipitation of the fulvic acid in the nutritional agent and improving the utilization rate of the nutritional agent.
Thirdly, the addition of the modifier can effectively improve the contact angle between the nutritional agent and the leaf surface of the oil peony, effectively improve the absorption of the oil peony on phosphorus, and further improve the oil yield of the oil peony; the bitter ginseng root is added with 5 times of N, N-dimethylformamide, 0.1 times of diethylenetriamine pentamethylene phosphonic acid and 0.01 times of phosphoric acid, thereby facilitating the release of effective components; b2, the impurities in the crude extract of the kushen root can be effectively removed by the operation treatment, and the influence of the impurities is reduced; the addition of Kochia scoparia extract can improve the dispersibility of soybean powder in water and slow down the coagulation of the soybean powder in aqueous solution.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
< example 1>
The nutrient for improving the oil yield of the peony for oil comprises the following raw materials in parts by weight: 5 parts of fulvic acid, 2 parts of monopotassium phosphate, 0.1 part of ammonium dihydrogen phosphate, 0.2 part of gibberellin, 5 parts of bitter melon seed extract, 0.1 part of chitosanase, 0.1 part of silicate bacteria, 20 parts of vermiculite powder, 6 parts of composite extracting solution and 3 parts of modifying agent;
wherein the modifying agent comprises the following raw materials in parts by weight in a mass ratio of 1:0.5:0.05:0.2: 1: sorbitol, sodium dodecylbenzene sulfonate, radix Sophorae Flavescentis extract, Kochiae fructus extract, and semen glycines powder;
the preparation method of the composite extracting solution comprises the following steps:
a1, mixing herba Leonuri, herba Menthae, rhizoma Alpiniae Officinarum, Capsici fructus branch and sweet potato leaf at a weight ratio of 1:4:1:10:30, cleaning with 75% ethanol solution for 3 times, cleaning with water for 2 times, freeze drying, pulverizing to obtain pulverized material, adding CO into the pulverized material2Adding 10 times of water, 2 times of acetone, 0.1 times of ethylenediamine and 0.1 times of sodium bicarbonate into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 95 ℃, extracting for 50-55 min, filtering and concentrating to obtain a first concentrate;
a2, adding dichloromethane in an amount which is 2 times of the total mass of the first concentrate, and then dropwise adding 1M hydrochloric acid solution until the first concentrate is completely dissolved to obtain a first mixed solution;
a3, purifying the first mixed solution by silica gel column chromatography, wherein an elution system is a petroleum ether-ethyl acetate system, collecting an eluate with a volume ratio of petroleum ether to ethyl acetate of 1: 0.9-1.5, concentrating to obtain a second concentrate, wherein a developing agent is petroleum ether and ethyl acetate with a volume ratio of 1:1, the Rf value of the second concentrate on a thin-layer plate is 0.2-0.6, the number of points is 8, collecting 8 point corresponding substances and concentrating to obtain the second concentrate;
and A4, adding water in an amount which is 3 times of the total mass of the second concentrate, and then adjusting the pH value to 7-8 by using a 4M potassium hydroxide aqueous solution to obtain a composite extracting solution.
The preparation method of the balsam pear seed extract comprises the following steps: mixing cleaned semen Momordicae Charantiae with 3 times of water, grinding at 70 deg.C for 10min to obtain slurry, adding 0.01 times of cellulase, hydrolyzing for 1.5h, heating to 100 deg.C to inactivate enzyme, adding 1 times of starch, stirring, gelatinizing at 70 deg.C for 20min, drying at 100 deg.C, and pulverizing to obtain semen Momordicae Charantiae extract.
The particle fineness of the vermiculite powder is 10-20 mu m.
The preparation method of the sophora flavescens root extract comprises the following steps:
b1, pulverizing radix Sophorae Flavescentis, and adding CO2Adding 10 times of water, 5 times of N, N-dimethylformamide, 0.1 times of diethylenetriamine pentamethylene phosphonic acid and 0.01 times of phosphoric acid into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 98 ℃, extracting for 60-75 min, filtering and concentrating to obtain a crude extract of the roots of the sophora flavescens;
and B2, adding methanol with the amount being 2 times of the total mass of the crude extract of the sophora flavescens roots into the crude extract of the sophora flavescens roots and dissolving the mixture to obtain a first standby solution, taking petroleum ether with the amount being 80 times of the total mass of the crude extract of the sophora flavescens roots, then dropwise adding the first standby solution into the petroleum ether under the stirring condition, standing for 5 hours after dropwise adding, and filtering to obtain the sophora flavescens roots extract.
The preparation method of the kochia scoparia extract comprises the following steps:
pulverizing Kochiae fructus, adding CO2And (3) adding water which is 10 times of that of the kochia scoparia into a supercritical extractor, extracting for 90-93 min at the extraction pressure of 20-22 MPa and the temperature of 75 ℃, filtering and concentrating to obtain the kochia scoparia extract.
The particle fineness of the soybean powder is 10-20 mu m.
< comparative example 1>
A nutritional agent was prepared according to the raw materials of example 1, except that the complex extract was not added to the nutritional agent.
< comparative example 2>
The nutrient is prepared according to the raw materials in the embodiment 1, except for the preparation method of the compound extracting solution, which comprises the following steps: mixing herba Leonuri, herba Menthae, rhizoma Alpiniae Officinarum, Capsici fructus branch and sweet potato leaf at a mass ratio of 1:4:1:10:30, cleaning with 75% ethanol solution for 3 times, and cleaning with waterFreeze drying and pulverizing for 2 times to obtain pulverized material, adding into CO2And (3) adding water which is 10 times of the total mass of the crushed materials into the supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 95 ℃, extracting for 50-55 min, filtering and concentrating to obtain a compound extracting solution, and keeping the rest unchanged.
< comparative example 3>
The nutrient is prepared according to the raw materials in the example 1, except for the preparation method of the balsam pear seed extract, which comprises the following steps: pulverizing semen Momordicae Charantiae, mixing with 30 times of water, heating at 100 deg.C for 5 hr, filtering, and concentrating to obtain semen Momordicae Charantiae extract, and keeping the rest materials.
< comparative example 4>
The nutritional agent was prepared according to the raw material of example 1, except that no modifier was added and the rest was unchanged.
< comparative example 5>
The nutrient is prepared according to the raw materials in the example 1, except that the preparation method of the extract of the sophora flavescens roots is different, and the method specifically comprises the following steps: pulverizing radix Sophorae Flavescentis, and adding CO2Adding 10 times of water into the supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 98 ℃, extracting for 60-75 min, filtering and concentrating to obtain the sophora flavescens root extract, and keeping the balance unchanged.
< comparative example 6>
The nutritional agent was prepared according to the raw materials of example 1, except that the kochia scoparia extract was not added, and the rest was unchanged.
Test of
1. Oil yield
Preparing 4 groups of nutritional agents according to the raw materials of the example 1 and the comparative examples 1-3, respectively spraying 4 groups of oil peonies with the nutritional agents in 3-6 months, wherein each group of oil peonies comprises 100 plants, treating the rest according to a conventional method, counting and calculating the oil yield and the unsaturated fatty acid content of the oil peonies in the groups corresponding to the example 1 and the comparative examples 1-3, and the results are shown in the table 1, wherein supercritical CO is adopted2Extracting the peony seed oil, and calculating the oil yield and the total unsaturated fatty acid content;
TABLE 1 oil yield
| Control group | Oil yield | Total unsaturated fatty acid content |
| Example 1 | 50.2％ | 97.6％ |
| Comparative example 1 | 45.3％ | 95.5％ |
| Comparative example 2 | 48.2％ | 96.1％ |
| Comparative example 3 | 48.9％ | 96.6％ |
The result shows that the complex action of fulvic acid and phosphorus can be promoted by adding the compound extracting solution into the nutritional agent, the absorption of plants to phosphorus is accelerated, and the oil yield of the oil peonies is further improved; the addition of the balsam pear seed extract is beneficial to improving the dispersibility of the fulvic acid, delaying the settlement of the fulvic acid in the nutritional agent and improving the utilization rate of the nutritional agent.
2. Determination of contact Angle
Dividing the nutritional agents of the embodiment 1 and the comparative examples 4-6 into four groups, and preparing the four groups of nutritional agents into solutions with the concentration of 2.1 g/L;
collecting fresh peony leaves for oil, cleaning the peony leaves for oil, selecting a flat part, shearing small leaf surfaces, horizontally placing the leaf surfaces in a sample groove of a contact angle meter, respectively sucking 2 mu L of the four groups of solutions by using an injector, dripping the solutions on the leaf surfaces at intervals, observing contact angles after 20s, repeating the steps for 10 times, and calculating an average value, wherein the result is shown in table 2;
TABLE 2 contact Angle
| Group of | Contact angle |
| Example 1 | 130.1° |
| Comparative example 4 | 126.8° |
| Comparative example 5 | 128.7° |
| Comparative example 6 | 127.4° |
The result shows that the addition of the modifying agent can effectively improve the contact angle between the nutritional agent and the leaf surface of the peony for oil and improve the utilization rate of the nutritional agent.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (6)
1. The nutrient for improving the oil yield of the peony for oil is characterized by comprising the following raw materials in parts by weight: 5 parts of fulvic acid, 2 parts of monopotassium phosphate, 0.1 part of ammonium dihydrogen phosphate, 0.2 part of gibberellin, 5 parts of bitter melon seed extract, 0.1 part of chitosanase, 0.1 part of silicate bacteria, 20 parts of vermiculite powder, 6 parts of composite extracting solution and 3 parts of modifying agent;
wherein the modifying agent comprises the following raw materials in parts by weight in a mass ratio of 1:0.5:0.05:0.2: 1: sorbitol, sodium dodecylbenzene sulfonate, radix Sophorae Flavescentis extract, Kochiae fructus extract, and semen glycines powder;
the preparation method of the composite extracting solution comprises the following steps:
a1, mixing herba Leonuri, herba Menthae, rhizoma Alpiniae Officinarum, Capsici fructus branch and sweet potato leaf at a weight ratio of 1:4:1:10:30, cleaning with 75% ethanol solution for 3 times, cleaning with water for 2 times, freeze drying, pulverizing to obtain pulverized material, adding CO into the pulverized material2Adding 10 times of water, 2 times of acetone, 0.1 times of ethylenediamine and 0.1 times of sodium bicarbonate into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 95 ℃, extracting for 50-55 min, filtering and concentrating to obtain a first concentrate;
a2, adding dichloromethane in an amount which is 2 times of the total mass of the first concentrate, and then dropwise adding 1M hydrochloric acid solution until the first concentrate is completely dissolved to obtain a first mixed solution;
a3, purifying the first mixed solution by silica gel column chromatography, wherein an elution system is a petroleum ether-ethyl acetate system, collecting an eluate with a volume ratio of petroleum ether to ethyl acetate of 1: 0.9-1.5, and concentrating to obtain a second concentrate;
and A4, adding water in an amount which is 3 times of the total mass of the second concentrate, and then adjusting the pH value to 7-8 by using a 4M potassium hydroxide aqueous solution to obtain a composite extracting solution.
2. The nutrient for improving the oil yield of the peony for oil according to claim 1, wherein the balsam pear seed extract is prepared by the following steps: mixing cleaned semen Momordicae Charantiae with 3 times of water, grinding at 70 deg.C for 10min to obtain slurry, adding 0.01 times of cellulase, hydrolyzing for 1.5h, heating to 100 deg.C to inactivate enzyme, adding 1 times of starch, stirring, gelatinizing at 70 deg.C for 20min, drying at 100 deg.C, and pulverizing to obtain semen Momordicae Charantiae extract.
3. The nutrient for improving the oil yield of the peony for oil according to claim 2, wherein the particle fineness of the vermiculite powder is 10 to 20 μm.
4. The nutrient for improving the oil yield of the peony for oil according to claim 1, wherein the preparation method of the extract of the sophora flavescens ait root comprises:
b1, pulverizing radix Sophorae Flavescentis, and adding CO2Adding 10 times of water, 5 times of N, N-dimethylformamide, 0.1 times of diethylenetriamine pentamethylene phosphonic acid and 0.01 times of phosphoric acid into a supercritical extractor, controlling the extraction pressure to be 20-22 MPa and the temperature to be 98 ℃, extracting for 60-75 min, filtering and concentrating to obtain a crude extract of the roots of the sophora flavescens;
and B2, adding methanol with the amount being 2 times of the total mass of the crude extract of the sophora flavescens roots into the crude extract of the sophora flavescens roots and dissolving the mixture to obtain a first standby solution, taking petroleum ether with the amount being 80 times of the total mass of the crude extract of the sophora flavescens roots, then dropwise adding the first standby solution into the petroleum ether under the stirring condition, standing for 5 hours after dropwise adding, and filtering to obtain the sophora flavescens roots extract.
5. The nutrient for improving the oil yield of the peony for oil according to claim 1, wherein the preparation method of the kochia scoparia extract comprises:
pulverizing Kochiae fructus, adding CO2Adding 10 times of water into a supercritical extractor, extracting for 90-93 min at 75 ℃ under 20-22 MPa, filtering and concentrating to obtain the kochia scopariaAnd (3) extracting.
6. The nutrient for improving the oil yield of the peony for oil according to claim 5, wherein the particle fineness of the soybean meal is 10 to 20 μm.
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