CN112174325A - Immobilized bioreactor, immobilization method and method for treating black smelly water - Google Patents
Immobilized bioreactor, immobilization method and method for treating black smelly water Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/308—Biological phosphorus removal
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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Abstract
The invention relates to the technical field of water body remediation equipment, in particular to a two-phase immobilized bioreactor, an immobilization method and a black and odorous water treatment method. The two-phase immobilized bioreactor comprises an aerobic denitrification reactor, an immobilized microalgae reactor and an oxygen tank for supplying oxygen to the aerobic denitrification reactor, wherein a liquid outlet of the aerobic denitrification reactor is communicated with a liquid inlet of the immobilized microalgae reactor, the aerobic denitrification reactor is provided with a first immobilized carrier and a wastewater inlet, and the immobilized microalgae reactor is provided with a second immobilized carrier and a wastewater outlet. The immobilized bioreactor can fix aerobic denitrifying bacteria and microalgae, and is used for treating black smelly water, so that the total nitrogen and total phosphorus in the wastewater can be effectively removed, and the purpose of purifying the water body is achieved; the method for treating black smelly water by the immobilized aerobic denitrifying bacteria and the immobilized microalgae is simple and efficient.
Description
Technical Field
The invention relates to the technical field of water body remediation equipment, in particular to a two-phase immobilized bioreactor, an immobilization method and a black and odorous water treatment method.
Background
With the development of social economy, the eutrophication of water bodies is increasingly serious, and the pollution problem of nitrogen and phosphorus in wastewater is more and more concerned. At present, the conventional wastewater denitrification adopts a biological method, but because the nitrification and denitrification processes are separately carried out, the treatment process is long, the occupied area is large, and the capital investment is high.
The biological method for treating the wastewater with the low C/N ratio has the problems of insufficient carbon source and low denitrification efficiency, the C/N ratios of chemical wastewater, culture wastewater, landfill leachate and part of municipal domestic sewage in China are low, and in the actual treatment process, an organic carbon source is usually added to improve the C/N ratio to realize the efficient removal of nitrogen, so that the wastewater treatment cost is increased, the greenhouse gas emission is increased, and the environment is polluted.
The traditional phosphorus removal process is divided into a chemical method and a biological method, and the chemical method needs to consume a large amount of medicament for phosphorus removal and generate a large amount of sludge. The phosphorus removal effect of the biological phosphorus removal method is not stable, and phosphorus accumulation bacteria need two reaction zones of anaerobic phosphorus release and aerobic phosphorus absorption, so that the treatment process is long, the occupied area is large, and the capital investment is high. In addition, sludge generated in the phosphorus removal process of the phosphorus-accumulating bacteria cannot be discharged in time, and phosphorus adsorbed before the phosphorus-accumulating bacteria die is released again, so that the treatment effect is influenced. And the traditional immobilized carrier material calcium alginate has the problems of easy decomposition and poor tolerance under the alkaline and high salinity conditions, and needs to be further improved.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention aims to provide a two-phase immobilized bioreactor and a method for treating black smelly water, wherein the immobilized bioreactor can be used for immobilizing aerobic denitrifying bacteria and immobilized microalgae and treating black smelly water, so that the total nitrogen and total phosphorus in wastewater can be effectively removed, and the purpose of purifying water is achieved; the method for treating black smelly water is simple and efficient.
The second object of the present invention is to provide a method for immobilizing aerobic denitrifying bacteria, which overcomes the above-mentioned disadvantages of the prior art. Aiming at the problems of insufficient carbon source and low denitrification efficiency in the biological treatment of the wastewater with the low C/N ratio, the method can have a good denitrification effect under the condition of low C/N by separating, domesticating and screening out a low C/N tolerant aerobic denitrifying bacterium from the environment; the immobilization method of the aerobic denitrifying bacteria can effectively solve the problems that the traditional immobilization carrier material calcium alginate is easy to decompose and poor in tolerance under the alkaline and high-salinity conditions.
The present invention also provides a method for immobilizing microalgae, which overcomes the above-mentioned shortcomings of the prior art. The invention has better phosphorus removal effect on the microalgae, uses the immobilization technology to ensure that the phosphorus can not be released again after being adsorbed, and uses the immobilization material to modify the immobilization material, thereby having better stability and tolerance compared with the traditional material. The microalgae immobilization method has the advantages of stable treatment effect, capability of effectively preventing secondary pollution and the like.
The purpose of the invention is realized by the following technical scheme: the utility model provides a two-phase formula immobilization bioreactor, includes good oxygen denitrification reactor, immobilization little algae reactor and is used for the oxygen cylinder for good oxygen denitrification reactor oxygen suppliment, good oxygen denitrification reactor's liquid outlet and immobilization little algae reactor's inlet intercommunication, good oxygen denitrification reactor is provided with first immobilization carrier and waste water import, immobilization little algae reactor is provided with second immobilization carrier and waste water export.
Further, be provided with first filtration baffle in the good oxygen denitrification reactor, first filtration baffle is used for separating the inner chamber of good oxygen denitrification reactor into the first immobilization room that is located first filtration baffle top and is located the filter chamber of first filtration baffle below, first immobilization carrier sets up in first immobilization indoor, the oxygen jar with cross the filter chamber intercommunication, the waste water import sets up in the filter chamber. The second filtering baffle can prevent the filler in the reactor from losing, and water can pass through the second filtering baffle.
Further, the oxygen tank is communicated with the aerobic denitrification reactor through a gas pipe, and the gas pipe is provided with a gas flowmeter. The first immobilization carrier is a polyurethane foam carrier. The flow rate of oxygen is controlled by a gas flowmeter to provide oxygen required by biochemical reaction for aerobic denitrifying bacteria in immobilized polyurethane foam, the concentration of dissolved oxygen is kept to be 3-5mg/L, so that the dissolved oxygen is subjected to heterotrophic nitrification and denitrification, and the concentrations of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen in wastewater can be effectively reduced through the metabolism of microorganisms.
Further, an air outlet is formed in the top end of the aerobic denitrification reactor, and an liquid outlet of the aerobic denitrification reactor is formed in the side wall of the first immobilization chamber.
Further, the filtering chamber is provided with a fence. The number of the fences is one or more rows. The fence can remove larger particles in the wastewater to prevent the aerobic denitrification reactor from being blocked. Furthermore, the immobilized polyurethane foam is provided with a back washing port, and the back washing port is arranged on the side wall of the filtering chamber and is positioned above the fence. The back washing port periodically washes larger particles in the fence through back washing water.
The wastewater enters the filtering chamber through a wastewater inlet arranged in the filtering chamber, the water level gradually rises, and when larger particles in the wastewater move upwards, the larger particles are blocked by the fence, so that the aerobic denitrification reactor can be prevented from being blocked by the larger particles; during back flushing, flushing water enters from the upper part of the fence and flushes down attachments attached to the fence, so that the cleaning is convenient.
Further, a second filtering baffle is arranged in the immobilized microalgae reactor, the second filtering baffle is used for dividing the inner cavity of the immobilized microalgae reactor into a second immobilization chamber located above the second filtering baffle and a liquid outlet chamber located below the second filtering baffle, the second immobilized carrier is arranged in the second immobilization chamber, the oxygen tank is communicated with the liquid outlet chamber, and the wastewater outlet is arranged in the liquid outlet chamber; the liquid inlet of the immobilized microalgae reactor is arranged on the side wall of the second immobilization chamber. The second filters the baffle and can prevent that the inside filler of reactor from running off, and the second filters the baffle and is provided with the pneumatic valve, and the waste water that has handled through biological filler can get into immobilization little algae reactor from this.
Furthermore, the immobilized microalgae reactor is provided with a stirrer and a light source for providing illumination, and the lower end of the stirrer extends into the second immobilized chamber.
Furthermore, the aerobic denitrification reactor and the immobilized microalgae reactor are provided with a pH meter, a liquid level sensor and a dissolved oxygen meter.
Furthermore, the aerobic denitrification reactor and the immobilized microalgae reactor are both provided with thermometers and heating rods respectively used for heating liquid in the aerobic denitrification reactor and the immobilized microalgae reactor.
Further, the first immobilization carrier is a polyurethane foam carrier. The invention uses polyurethane foam as an immobilized carrier to immobilize aerobic denitrifying bacteria, and has the characteristics of better hydrophilicity, pore structure, microbial affinity, biodegradability resistance and the like.
Further, the second immobilization carrier is an immobilized microalgae bead.
The two-phase immobilized bioreactor is a bioreactor for treating black and odorous water by two-item immobilized microalgae and immobilized aerobic denitrifying bacteria. The two-phase immobilized bioreactor utilizes aerobic denitrifying bacteria fixed on polyurethane foam to remove ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen in wastewater, the screened low-C/N-resistant aerobic denitrifying bacteria have a good denitrification effect in lower C/N wastewater, and the polyurethane foam is used as an immobilized carrier to immobilize the aerobic denitrifying bacteria, so that the two-phase immobilized bioreactor has the characteristics of good hydrophilicity, pore structure, microbial affinity, biodegradability resistance and the like. Fixing microalgae in a carrier, removing phosphorus-containing compounds in the wastewater by using the metabolism of the microalgae, generating carbon dioxide as an algae carbon source by the respiration of microorganisms outside the carrier, and realizing win-win by the interaction of the microalgae and bacteria through nutrition exchange, signal transduction, gene transfer and the like so as to achieve the purpose of efficiently treating the total phosphorus in the wastewater. The second immobilized carrier is a material obtained by modifying polyvinyl alcohol on the basis of sodium alginate, the physical and chemical properties and the antimicrobial decomposability of the material are enhanced, and the material has the advantages of low cost, high effect, strong tolerance, no toxicity or harm to the environment and the like. The invention has low operation cost, is environment-friendly and has no secondary pollution. The method for treating black smelly water by the immobilized aerobic denitrifying bacteria and the immobilized microalgae is simple and efficient.
The invention also provides a method for treating black odorous water, which adopts the two-phase immobilized bioreactor and comprises the following steps:
(1) conveying the wastewater to be treated into an aerobic denitrification reactor from a wastewater inlet, treating the wastewater by aerobic denitrifying bacteria fixed on a first immobilized carrier, and then feeding the wastewater into an immobilized microalgae reactor; the oxygen tank provides oxygen required by the aerobic denitrifying bacteria;
(2) after the wastewater treated by the aerobic denitrification reactor enters the immobilized microalgae reactor, treating the wastewater by microalgae arranged on a second immobilized carrier; the treated wastewater is discharged from a wastewater outlet.
When the two-phase immobilized bioreactor disclosed by the invention is used for treating black odorous water, wastewater to be treated enters the aerobic denitrification reactor from the wastewater inlet, the fence can remove larger particles in the wastewater to prevent the reactor from being blocked, the first immobilized carrier is an immobilized polyurethane carrier, the carrier is polyurethane foam, and the aerobic denitrification bacteria strain is low-C/N-resistant aerobic denitrification bacteria; the flow rate of oxygen in an oxygen tank is controlled by a gas flowmeter to provide oxygen required by biochemical reaction for aerobic denitrifying bacteria in immobilized polyurethane foam, the concentration of dissolved oxygen is kept to be 3-5mg/L, so that heterotrophic nitrification and denitrification are performed, the concentrations of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen in wastewater can be effectively reduced under the metabolism of microorganisms, and a backwashing port periodically cleans larger particles in a fence through backwashing water; the wastewater enters the immobilized microalgae reactor after being treated by the aerobic denitrification reactor, a second immobilized carrier is arranged in the immobilized microalgae reactor, the second immobilized carrier is immobilized microalgae beads, the second immobilized carrier is a material for modifying sodium alginate by adopting polyvinyl alcohol, microorganisms embedded in the second immobilized microorganism beads are microalgae, and the concentration of phosphorus compounds in the wastewater can be reduced by the metabolic action of the microalgae. The stirrer enables the immobilized microalgae beads in the immobilized microalgae reactor to rotate at a certain speed, and the light source provides light source for the microalgae in the immobilized microalgae reactor. The pH meters of the aerobic denitrification reactor and the immobilized microalgae reactor monitor the pH value of the water body to ensure that the pH value in the wastewater can be kept between 6.5 and 8, the dissolved oxygen meter controls the concentration of dissolved oxygen in the water body, the thermometer and the heating rod keep the internal temperature of the immobilized microalgae reactor to be kept between 25 and 30 ℃, the liquid level sensor monitors the water level to keep the water level not to exceed 2/3 of the total volume, the stability and the safety of the reactor can be kept by controlling the water level, the overflow is easy when the water level is too high, gas generated in the reaction process when the water level is too low in treatment effect is discharged from a gas outlet, and the treated wastewater is directly discharged.
Aerobic denitrifying bacteria in the immobilized polyurethane foam carrier convert nitrate nitrogen and nitrite nitrogen into nitrogen in an aerobic environment and simultaneously perform heterotrophic nitrification reaction, convert ammonia nitrogen into amide and finally convert the amide into the nitrogen, and realize the removal of nitrogen elements in wastewater. The microalgae effectively remove the phosphorus-containing compounds in the wastewater by using the metabolism of the microalgae. The immobilization of the microalgae can increase the biomass concentration of the microalgae and the tolerance capability to the environment, and can also prevent the microalgae from losing. And (4) discharging the wastewater from the gas outlet, and directly discharging the treated wastewater. The two-phase immobilized microalgae and immobilized aerobic denitrifying bacteria bioreactor for treating black odorous water has a nitrogen and phosphorus treatment effect of 98% on wastewater.
The invention also provides an immobilization method of the aerobic denitrifying bacteria, which comprises the following steps:
A. preparing a polyurethane foam carrier;
B. polyurethane foam is adopted to immobilize aerobic denitrifying bacteria.
Further, in the step a, the preparation method of the polyurethane foam carrier includes the following steps: ultrasonically dispersing polyester polyol, triethanolamine, water, methyl silicone oil, dibutyltin dilaurate and crushed graphite felt powder together, and uniformly stirring; adding diphenylmethane diisocyanate (MDI), stirring until the mixture turns white, and quickly transferring the mixture to naturally foam for 23 to 25 hours to prepare the first-class aperture polyurethane foam. And removing graphite felt powder in the preparation process to obtain the second type of aperture polyurethane foam.
Further, in the step a, the preparation method of the polyurethane foam carrier includes the following steps: according to the weight portion, 37 to 43 portions of polyester polyol, 1.5 to 2.5 portions of triethanolamine, 1.5 to 2.5 portions of water, 1.5 to 2.5 portions of methyl silicone oil, 1.5 to 2.5 portions of dibutyltin dilaurate and 1.6 to 2.4 portions of crushed graphite felt powder (3 pieces are 3 multiplied by 3cm each time) are ultrasonically dispersed for 5 to 10 seconds and then are evenly stirred; adding 47-53 parts of diphenylmethane diisocyanate (MDI), stirring for 3-5s until the mixture turns white, and quickly transferring to naturally foam for 24h to prepare the first-class pore-size polyurethane foam. The two types of polyurethane foams prepared were cut into rectangular blocks of 1cm × 1cm × 0.5cm, and the pore diameters were counted and measured, with the range of pore diameters of the first type of polyurethane foam being 2.74 ± 0.56mm (denoted as large pore diameter) and the range of pore diameters of the second type of polyurethane foam being 1.18 ± 0.33mm (denoted as small pore diameter).
The most commonly used immobilization carriers are calcium alginate in solutions containing high concentrations of phosphate or gluconate, or Mg2+、K+The calcium alginate gel is unstable in the cation solution necessary for the growth of the microorganismEasy to break and dissolve. Therefore, the invention uses the novel material polyurethane foam to immobilize the aerobic denitrifying bacteria and modify the aerobic denitrifying bacteria.
Further, the step B specifically includes: washing the polyurethane foam with distilled water for several times, completely drying the polyurethane foam, preparing an LB culture medium, sterilizing the polyurethane foam and the culture medium together (12 cuboid blocks are added into 100mL of the culture medium), cooling to room temperature, inoculating a strain to be immobilized (aerobic denitrifying bacteria), performing shaking culture at the temperature of 28-31 ℃ and the speed of 110-.
Further, in the step B, the aerobic denitrifying bacteria are low C/N resistant aerobic denitrifying bacteria, and the screening and domesticating method of the aerobic denitrifying bacteria comprises the following steps:
b1, inoculating 1 wt% black and odorous water into an enrichment medium for culture, wherein the culture conditions are as follows: culturing at 25-30 deg.C, pH 7-9 and rotation speed of 150-.
And B2, inoculating the enrichment medium suspension in the logarithmic phase onto LB solid medium for culture until clear colonies grow on the surface of the LB solid medium. A single colony on the LB solid culture medium is inoculated to a bromothymol blue denitrification solid culture medium for culture, and the bromothymol blue denitrification solid culture medium shows that the screened strain is denitrifying bacteria (named bacteria A, B, C, D and the like) when the color is changed from red to blue. Bromothymol blue is an acid-base indicator, and when the pH of the culture medium is changed from neutral to alkaline, the culture medium is changed from red to blue.
B3, inoculating test strains (such as bacteria A, B, C, D) into a bromothymol blue denitrification solid culture medium, controlling C/N (0.5, 1, 2, 3 and 4), and keeping other conditions unchanged, wherein the bromothymol blue denitrification solid culture medium shows that the screened strains are low C/N resistant bacteria when the color is changed from red to blue.
And B4, performing a removal test on the screened aerobic denitrifying bacteria by using simulated wastewater (low C/N), wherein the high removal rate is the target strain.
Wherein, in the step B1, the enrichment medium formula comprises: 5g/L of peptone and 5g/L, NaCl 10g/L of yeast extract powder.
In the step B2, the formulation of the bromothymol blue denitrification solid medium comprises: CH (CH)3COONa 5g/L，KH2PO4 0.0659g/L，KNO3 0.722g/L，MgSO4·7H20.20g/L of O, 15-20g/L of agar, 1% of bromothymol blue and 2% of trace elements, wherein the trace elements comprise: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)2·6H2O 49.4g/L。
The biological method for treating the wastewater with the low C/N ratio has the problems of insufficient carbon source and low denitrification efficiency, and the C/N ratios of chemical wastewater, culture wastewater, landfill leachate and part of municipal domestic sewage in China are lower, so that the method is designed for screening the aerobic denitrifying bacteria with low C/N resistance to enhance the denitrification efficiency of the wastewater.
The invention also provides a microalgae immobilization method, which comprises the following steps:
s1, inoculating the scenedesmus obliquus and chlorella vulgaris seed liquid to BG11 culture medium under aseptic operation condition to obtain algae liquid;
s2, centrifuging the algae liquid in the logarithmic growth phase in a refrigerated centrifuge, discarding supernatant, washing algae bodies with sodium bicarbonate solution, removing nutritive salt adsorbed on the surfaces of algae cells, washing with sterile water, transferring the algae bodies to a nitrogen-free culture medium for culture to exhaust the nutritive salt in the algae cells, and finally centrifuging to obtain an algae cell concentrated solution;
s3, mixing the algae cell concentrated solution with sodium alginate and polyvinyl alcohol solution which are sterilized in advance, and uniformly stirring to form mixed solution; to CaCl2–H3BO3And dripping the mixed solution on the solution surface to form the microalgae spheres, and standing and fixing in a refrigerator at the temperature of 2-6 ℃ to obtain the immobilized microalgae spheres.
The microalgae culturing and immobilizing method provided by the invention is characterized in that the microalgae is cultured by controlling the culture conditions and adopting an illumination circulation alternating method, after the microalgae is immobilized by the method, the immobilized materials are all materials with good hydrophilicity, pore structure, microbial affinity and biodegradability resistance, and the immobilized microalgae is prepared by modifying the materials, so that the phosphorus removal capability is improved, and the immobilized microalgae culturing and immobilizing method has the advantages of high efficiency, strong poison resistance, stable and reliable operation, less microalgae cell loss, easiness in product separation and the like, and can effectively prevent secondary pollution of treated water.
Further, in step S2, the BG11 medium includes the following raw materials: NaNO3 1.5g/L，K2HPO40.04g/L，MgSO4·7H2O 0.075g/L，CaCl2·7H2O 0.036g/L，Na2CO30.02g/L, 0.006g/L citric acid, 0.006g/L ferric citrate, and 1mL/L trace element solution, wherein the trace element solution comprises: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)26H2O 49.4g/L。
Further, the step S2 is specifically: will be in logarithmic growth phase (microbial biomass concentration 1X 10)∧6-1×10∧7cfu/mL) in a high-speed refrigerated centrifuge at 4 deg.C and 3500 r.min-1Centrifuging for 10min, discarding supernatant, and treating with 15 mg. L-1NaHCO of3Washing algae with the solution to remove nutrient salt adsorbed on the surface of algae cells, washing with sterile water, transferring to nitrogen-free culture medium, culturing for 3 days to exhaust nutrient salt in the algae cells, and centrifuging to obtain algae cell concentrated solution.
Further, the step S3 is specifically: taking the concentrated solution of the algae cells, and the sodium alginate and polyvinyl alcohol solution which are sterilized in advance according to the volume ratio of 1: 1 mixing and stirring to form a mixed solution, injecting the mixed solution into a 60mL syringe, and adding the mixed solution into precooled CaCl2–H3BO3And (3) dripping the mixed solution at the position of 20cm of the solution liquid level to form the algal balls with the diameter of 4mm, standing and fixing in a refrigerator at the temperature of 4 ℃ for 24 hours to prepare the immobilized microalgae pellets. Diameter of said algal sphereAbout 4 mm.
Further, the sodium alginate solution has the concentration of 2-4 wt%, the polyvinyl alcohol has the preferred concentration of 6-10 wt%, the CaCl2 has the preferred concentration of 1.5-2.5 wt%, and H3BO3The preferred concentration is 5-7 wt%.
Further, the sodium alginate solution preferably has a concentration of 3 wt%, the polyvinyl alcohol preferably has a concentration of 8 wt%, the CaCl2 preferably has a concentration of 2 wt%, and H3BO3The preferred concentration is 5.6 wt%.
The second immobilized carrier is a material obtained by modifying polyvinyl alcohol on the basis of sodium alginate, the physical and chemical properties and the antimicrobial decomposability of the material are enhanced, and the material has the advantages of low cost, high effect, strong tolerance, no toxicity or harm to the environment and the like. The invention has low operation cost, is environment-friendly and has no secondary pollution.
The invention has the beneficial effects that:
(1) the immobilized bioreactor can fix aerobic denitrifying bacteria and immobilized microalgae, is used for treating black smelly water, realizes effective removal of total nitrogen and total phosphorus in wastewater, and achieves the aim of purifying water; the method for treating black smelly water is simple and efficient. The method utilizes aerobic denitrifying bacteria fixed on a first immobilized carrier to remove ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen in the wastewater; fixing the microalgae in a second immobilized carrier, removing phosphorus-containing compounds in the wastewater by using the metabolism of the microalgae, generating carbon dioxide as an algae carbon source by the respiration of microorganisms outside the carrier, and realizing win-win by the interaction of the microalgae and bacteria through nutrition exchange, signal transduction, gene transfer and the like so as to achieve the purpose of efficiently treating the total phosphorus in the wastewater.
(2) The invention uses polyurethane foam as an immobilized carrier to immobilize aerobic denitrifying bacteria, and has the characteristics of better hydrophilicity, pore structure, microbial affinity, biodegradability resistance and the like; aerobic denitrifying bacteria in the immobilized polyurethane foam carrier convert nitrate nitrogen and nitrite nitrogen into nitrogen in an aerobic environment and simultaneously perform heterotrophic nitrification reaction, convert ammonia nitrogen into amide and finally convert the amide into the nitrogen, and realize the removal of nitrogen elements in wastewater.
(3) The immobilized microalgae method provided by the invention is used for culturing microalgae by controlling the culture condition and the illumination circulation alternation method, the microalgae is immobilized by the method, and the immobilized materials are all materials with better hydrophilicity, pore structure, microbial affinity and biodegradability resistance, and the immobilized microalgae is prepared by modifying the materials, so that the phosphorus removal capability is improved, and the immobilized microalgae method has the advantages of high efficiency, strong poison resistance, stable and reliable operation, less microalgae cell loss, easiness in product separation and the like, and can effectively prevent secondary pollution of treated water.
Drawings
Fig. 1 is a schematic structural view of the present invention.
The reference signs are: 1-aerobic denitrification reactor, 2-oxygen tank, 3-gas type flowmeter, 4-wastewater inlet, 6-first filtering partition, 7-first immobilized carrier, 8-back flushing port, 9-heating rod, 10-gas outlet, 11-pH meter, 12-dissolved oxygen meter, 13-thermometer, 14-liquid level sensor, 15-immobilized microalgae reactor, 16-stirrer, 17-immobilized microalgae pellet, 18-second filtering partition, 19-filtering chamber and 20-liquid outlet chamber.
Detailed Description
For the understanding of those skilled in the art, the present invention will be further described with reference to the following examples and the accompanying fig. 1, and the description of the embodiments is not intended to limit the present invention.
In a typical embodiment of the present invention, a two-phase immobilized bioreactor is provided, which comprises an aerobic denitrification reactor 1, an immobilized microalgae reactor 15 and an oxygen tank 2 for supplying oxygen to the aerobic denitrification reactor 1, wherein an outlet of the aerobic denitrification reactor 1 is communicated with an inlet of the immobilized microalgae reactor 15, the aerobic denitrification reactor 1 is provided with a first immobilized carrier 7 and a wastewater inlet 4, and the immobilized microalgae reactor 15 is provided with a second immobilized carrier and a wastewater outlet.
Further, be provided with first filtration baffle 6 in the good oxygen denitrification reactor 1, first filtration baffle 6 is used for separating into the first immobilization room that is located first filtration baffle 6 top and is located the filter chamber 19 of first filtration baffle 6 below with the inner chamber of good oxygen denitrification reactor 1, first immobilization carrier 7 sets up in first immobilization indoor, oxygen jar 2 and the room intercommunication of worrying about, waste water import 4 sets up in filter chamber 19.
The second filtering baffle 18 prevents the loss of packing inside the reactor and water can pass through the second filtering baffle 18.
Further, the oxygen tank 2 is communicated with the aerobic denitrification reactor 1 through a gas pipe, and the gas pipe is provided with a gas flowmeter 3. The first immobilization carrier 7 is a polyurethane foam carrier. The flow rate of the oxygen is controlled by the gas flowmeter 3 to provide oxygen required by biochemical reaction for aerobic denitrifying bacteria in the immobilized polyurethane foam, the concentration of dissolved oxygen is kept to be 3-5mg/L, so that the dissolved oxygen is subjected to heterotrophic nitrification and denitrification, and the concentrations of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen in the wastewater can be effectively reduced through the metabolism of microorganisms.
Further, an air outlet 10 is arranged at the top end of the aerobic denitrification reactor 1, and an liquid outlet of the aerobic denitrification reactor 1 is arranged on the side wall of the first immobilization chamber.
Further, the filtering chamber 19 is provided with a fence. The number of the fences is one or more rows. The fence can remove larger particles in the wastewater to prevent the aerobic denitrification reactor 1 from being blocked. Further, the immobilized polyurethane foam is provided with a back washing port 8. The back flushing port 8 is arranged on the side wall of the filtering chamber 19 and is positioned above the fence. The back flushing port 8 periodically cleans the fence of larger particles through back flushing water.
Further, a second filtering partition plate 18 is arranged in the immobilized microalgae reactor 15, the second filtering partition plate 18 is used for dividing the inner cavity of the immobilized microalgae reactor 15 into a second immobilization chamber located above the second filtering partition plate 18 and a liquid outlet chamber 20 located below the second filtering partition plate 18, the second immobilized carrier is arranged in the second immobilization chamber, the oxygen tank 2 is communicated with the liquid outlet chamber 20, and the wastewater outlet is arranged in the liquid outlet chamber 20; the liquid inlet of the immobilized microalgae reactor 15 is arranged on the side wall of the second immobilization chamber.
Further, the immobilized microalgae reactor 15 is provided with a stirrer 16 and a light source for providing illumination, and the lower end of the stirrer 16 extends into the second immobilization chamber.
Further, the aerobic denitrification reactor 1 and the immobilized microalgae reactor 15 are both provided with a pH meter 11, a liquid level sensor 14 and a dissolved oxygen meter 12.
Further, the aerobic denitrification reactor 1 and the immobilized microalgae reactor 15 are both provided with a thermometer 13 and a heating rod 9 for heating the liquid in the aerobic denitrification reactor 1 and the immobilized microalgae reactor 15 respectively.
In one embodiment of the present invention, the first immobilization carrier 7 is a polyurethane foam carrier. The invention uses polyurethane foam as an immobilized carrier to immobilize aerobic denitrifying bacteria, and has the characteristics of better hydrophilicity, pore structure, microbial affinity, biodegradability resistance and the like.
In one embodiment of the present invention, the second immobilization carrier is immobilized microalgae beads 17. The reaction zone, i.e., the second immobilization chamber, had a packed amount of immobilized beads of 30%.
In one embodiment of the invention, a gas flow meter is arranged between the oxygen tank 2 and the aerobic denitrification reactor 1 to control the oxygen content in the water body. The concentration of dissolved oxygen is kept to be 3-5 mg/L.
In one embodiment of the invention, the temperature of the thermometer 13 and the heating rod 9 is controlled so that the internal temperature of the aerobic denitrification reactor 1 and the immobilized microalgae reactor 15 is kept at 25-30 ℃.
In one embodiment of the present invention, the level sensor 14 monitors the water level to keep the water level not exceeding the total volume 2/3 of the aerobic denitrification reactor 1 and the immobilized microalgae reactor 15.
In one embodiment of the invention, the pH meter 11 monitors the pH value of the water body to maintain the pH value in the wastewater to be 6.5-8.
According to the inventionIn one embodiment, the light source is a high-efficiency fluorescent lamp, and the illumination intensity of the light source is 1.5 × 10^4lux。
In one embodiment of the present invention, the microalgae embedded in the immobilized microalgae beads 17 is Scenedesmus obliquus or Chlorella vulgaris. The Scenedesmus obliquus and the chlorella vulgaris are microalgae with a good effect of removing total phosphorus.
The invention also provides a method for treating black odorous water, which adopts the two-phase immobilized bioreactor and comprises the following steps:
(1) conveying the wastewater to be treated into an aerobic denitrification reactor 1 from a wastewater inlet 4, treating the wastewater by aerobic denitrifying bacteria fixed on a first immobilized carrier 7, and then feeding the wastewater into an immobilized microalgae reactor 15; the oxygen tank 2 provides oxygen required by the aerobic denitrifying bacteria;
(2) after the wastewater treated by the aerobic denitrification reactor 1 enters the immobilized microalgae reactor 15, the wastewater is treated by microalgae arranged on a second immobilized carrier; the treated wastewater is discharged from a wastewater outlet.
In one embodiment of the present invention, a method for immobilizing aerobic denitrifying bacteria includes the steps of:
A. preparing a polyurethane foam carrier;
B. polyurethane foam is adopted to immobilize aerobic denitrifying bacteria.
Further, in the step a, the preparation method of the polyurethane foam carrier includes the following steps: according to the weight portion, 37 to 43 portions of polyester polyol, 1.5 to 2.5 portions of triethanolamine, 1.5 to 2.5 portions of water, 1.5 to 2.5 portions of methyl silicone oil, 1.5 to 2.5 portions of dibutyltin dilaurate and 1.6 to 2.4 portions of crushed graphite felt powder (3 pieces are 3 multiplied by 3cm each time) are ultrasonically dispersed for 5 to 10 seconds and then are evenly stirred; adding 47-53 parts of diphenylmethane diisocyanate (MDI), stirring until the mixture turns white, transferring, and naturally foaming for 23-25h to prepare the first-class aperture polyurethane foam. And removing graphite felt powder in the preparation process to obtain the second type of aperture polyurethane foam.
Further, the step B specifically includes: washing the polyurethane foam with distilled water for several times, completely drying the polyurethane foam, preparing an LB culture medium, sterilizing the polyurethane foam and the culture medium together (12 cuboid blocks are added into 100mL of the culture medium), cooling to room temperature, inoculating a strain to be immobilized (aerobic denitrifying bacteria), performing shaking culture at 30 ℃, 130rpm for 3d, and washing the immobilized polyurethane foam with sterilized deionized water for several times for later use.
In one embodiment of the present invention, in the step a, the preparation method of the polyurethane foam carrier includes the following steps: according to the weight portion, 37 to 43 portions of polyester polyol, 1.5 to 2.5 portions of triethanolamine, 1.5 to 2.5 portions of water, 1.5 to 2.5 portions of methyl silicone oil, 1.5 to 2.5 portions of dibutyltin dilaurate and 1.6 to 2.4 portions of crushed graphite felt powder (3 pieces are 3 multiplied by 3cm each time) are ultrasonically dispersed for 5 to 10 seconds and then are evenly stirred; adding 47-53 parts of diphenylmethane diisocyanate (MDI), stirring for 3-5s until the mixture turns white, and quickly transferring for natural foaming for 23-25h to prepare the first-class aperture polyurethane foam. The two types of polyurethane foams prepared were cut into rectangular blocks of 1cm × 1cm × 0.5cm, and the pore diameters were counted and measured, with the range of pore diameters of the first type of polyurethane foam being 2.74 ± 0.56mm (denoted as large pore diameter) and the range of pore diameters of the second type of polyurethane foam being 1.18 ± 0.33mm (denoted as small pore diameter).
In an embodiment of the present invention, in the step B, the aerobic denitrifying bacteria are low C/N-tolerant aerobic denitrifying bacteria, and the screening and domestication method of the aerobic denitrifying bacteria includes the following steps:
b1, inoculating 1 wt% black and odorous water into an enrichment medium for culture, wherein the culture conditions are as follows: culturing at 25-30 deg.C, pH 7-9 and rotation speed of 150-.
And B2, inoculating the enrichment medium suspension in the logarithmic phase onto LB solid medium for culture until clear colonies grow on the surface of the LB solid medium. A single colony on the LB solid culture medium is inoculated to a bromothymol blue denitrification solid culture medium for culture, and the bromothymol blue denitrification solid culture medium shows that the screened strain is denitrifying bacteria (named bacteria A, B, C, D and the like) when the color is changed from red to blue. Bromothymol blue is an acid-base indicator, and when the pH of the culture medium is changed from neutral to alkaline, the culture medium is changed from red to blue.
B3, inoculating test strains (such as bacteria A, B, C, D) into a bromothymol blue denitrification solid culture medium, controlling C/N (0.5, 1, 2, 3 and 4), and keeping other conditions unchanged, wherein the bromothymol blue denitrification solid culture medium shows that the screened strains are low C/N resistant bacteria when the color is changed from red to blue.
And B4, performing a removal test on the screened aerobic denitrifying bacteria by using simulated wastewater (low C/N), wherein the high removal rate is the target strain.
In one embodiment of the present invention, in the step B1, the enrichment medium formula includes: 5g/L of peptone and 5g/L, NaCl 10g/L of yeast extract powder.
In one embodiment of the present invention, in the step B2, the formulation of the bromothymol blue denitrification solid medium comprises: CH (CH)3COONa 5g/L，KH2PO4 0.0659g/L，KNO3 0.722g/L，MgSO4·7H20.20g/L of O, 15-20g/L of agar, 1% of bromothymol blue and 2% of trace elements, wherein the medium and trace elements comprise: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)2·6H2O 49.4g/L。
In one embodiment of the present invention, a method for immobilizing microalgae includes the following steps:
s1, inoculating the scenedesmus obliquus and chlorella vulgaris seed liquid to BG11 culture medium under aseptic operation condition to obtain algae liquid;
s2, centrifuging the algae liquid in the logarithmic growth phase in a refrigerated centrifuge, discarding supernatant, washing algae bodies with sodium bicarbonate solution, removing nutritive salt adsorbed on the surfaces of algae cells, washing with sterile water, transferring the algae bodies to a nitrogen-free culture medium for culture to exhaust the nutritive salt in the algae cells, and finally centrifuging to obtain an algae cell concentrated solution;
s3, mixing the algae cell concentrated solution with sodium alginate and polyvinyl alcohol solution which are sterilized in advance, and uniformly stirring to form mixed solution; to CaCl2–H3BO3And dripping the mixed solution on the solution surface to form the microalgae spheres, and standing and fixing in a refrigerator at the temperature of 2-6 ℃ to obtain the immobilized microalgae spheres 17.
Further, in step S2, the BG11 medium includes the following raw materials: NaNO3 1.5g/L，K2HPO40.04g/L，MgSO4·7H2O 0.075g/L，CaCl2·7H2O 0.036g/L，Na2CO30.02g/L, 0.006g/L citric acid, 0.006g/L ferric citrate, and 1mL/L trace element solution, wherein the trace element solution comprises: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)26H2O 49.4g/L。
Further, the step S2 is specifically: will be in logarithmic growth phase (microbial biomass concentration 1X 10)∧6-1×10∧7cfu/mL) in a high-speed refrigerated centrifuge at 4 deg.C and 3500 r.min-1Centrifuging for 10min, discarding supernatant, and treating with 15 mg. L-1NaHCO of3Washing algae with the solution to remove nutrient salt adsorbed on the surface of algae cells, washing with sterile water, transferring to nitrogen-free culture medium, culturing for 3 days to exhaust nutrient salt in the algae cells, and centrifuging to obtain algae cell concentrated solution.
Further, the step S3 is specifically: taking the concentrated solution of the algae cells, and the sodium alginate and polyvinyl alcohol solution which are sterilized in advance according to the volume ratio of 1: 1 mixing and stirring to form a mixed solution, injecting the mixed solution into a 60mL syringe, and adding the mixed solution into precooled CaCl2–H3BO3And (3) dripping the mixed solution at the position of 20cm of the solution liquid level to form an algal ball with the diameter of 4mm, standing and fixing in a refrigerator at the temperature of 4 ℃ for 24 hours to prepare the immobilized microalgae beads 17. The diameter of the algal sphere is about 4 mm.
One implementation of the inventionIn the mode, the sodium alginate solution preferably has the concentration of 3 wt%, the polyvinyl alcohol preferably has the concentration of 8 wt%, the CaCl2 preferably has the concentration of 2 wt%, and H3BO3The preferred concentration is 5.6 wt%.
Example 1
An immobilization method of aerobic denitrifying bacteria comprises the following steps:
A. preparing a polyurethane foam carrier;
B. polyurethane foam is adopted to immobilize aerobic denitrifying bacteria.
Further, in the step a, the preparation method of the polyurethane foam carrier includes the following steps: : according to the parts by weight, 40 parts of polyester polyol, 2 parts of triethanolamine, 2 parts of water, 2 parts of methyl silicone oil, 2 parts of dibutyltin dilaurate and 2 parts of crushed graphite felt powder (3 pieces of 3 multiplied by 3cm each time) are ultrasonically dispersed for 8s and then are uniformly stirred; adding 50 parts of diphenylmethane diisocyanate (MDI), stirring for 3-5s until the mixture turns white, and quickly transferring for natural foaming for 24h to prepare the first-class pore-size polyurethane foam. The two types of polyurethane foams prepared were cut into rectangular blocks of 1cm × 1cm × 0.5cm, and the pore diameters were counted and measured, with the range of pore diameters of the first type of polyurethane foam being 2.74 ± 0.56mm (denoted as large pore diameter) and the range of pore diameters of the second type of polyurethane foam being 1.18 ± 0.33mm (denoted as small pore diameter).
Further, the step B specifically includes: washing the polyurethane foam with distilled water for several times, completely drying the polyurethane foam, preparing an LB culture medium, sterilizing the polyurethane foam and the culture medium together (12 cuboid blocks are added into 100mL of the culture medium), cooling to room temperature, inoculating a strain to be immobilized (aerobic denitrifying bacteria), performing shaking culture at 30 ℃, 130rpm for 3d, and washing the immobilized polyurethane foam with sterilized deionized water for several times for later use.
Example 2
A screening and domesticating method of low C/N resistant aerobic denitrifying bacteria comprises the following steps:
b1, inoculating 1% of black and odorous water into an enrichment medium for culture to obtain an enrichment medium suspension;aims to enrich bacteria in the bottom sediment and facilitate subsequent screening. The preferred concentration of total phosphorus tested by the invention is 2-5mg/L, NO3 --N preferably at a concentration of 20-30mg/L, NO2 -N is preferably 5-10mg/L, NH4 +The preferred concentration of-N is 30-35 mg/L. The formula of the enrichment medium is preferably 5g/L of peptone and 5g/L, NaCl 10g/L of yeast extract powder.
And B2, inoculating the enrichment medium suspension in logarithmic phase (OD ≧ 0.8) to an LB solid medium for culture until a clear colony grows on the surface of the LB solid medium. A single colony (named as A, B, C, D and the like) on the LB solid culture medium is inoculated to a bromothymol blue denitrification solid culture medium for culture, and the bromothymol blue denitrification solid culture medium shows that the screened strain is denitrifying bacteria when the color is changed from red to blue. The preferable formula of bromothymol blue denitrification solid culture medium is CH3COONa 5g/L，KH2PO4 0.0659g/L，KNO3 0.722g/L，MgSO4·7H20.20g/L of O, 15-20g/L of agar, 1% of bromothymol blue and 2% of trace elements, wherein the trace elements comprise: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)2·6H2O 49.4g/L。
B3, inoculating test strains (such as bacteria A, B, C, D) into a bromothymol blue denitrification solid culture medium, controlling C/N (0.5, 1, 2, 3 and 4), and keeping other conditions unchanged, wherein the bromothymol blue denitrification solid culture medium shows that the screened strains are low C/N resistant bacteria when the color is changed from red to blue.
And B4, performing a removal test on the screened aerobic denitrifying bacteria by using simulated wastewater (low C/N), wherein the high removal rate is the target strain.
Further, the formulation of the denitrification liquid culture medium is preferably CH3COONa 5g/L，KH2PO4 0.0659g/L，KNO30.722g/L，MgSO4·7H20.20g/L of O, 1 percent of bromothymol blue and 2 percent of trace elements, whereinThe quantity elements comprise: h3BO42.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)2·6H2O49.4 g/L. The nitrogen and phosphorus removal capability and the growth curve of the strain are in positive correlation, namely the highest phosphorus removal rate is achieved at the end of the logarithmic phase or the logarithmic phase.
Example 3
A method for culturing and immobilizing microalgae comprises the following steps:
s1, inoculating the Scenedesmus obliquus and chlorella vulgaris seed liquid into BG11 culture medium under aseptic operation conditions, wherein the culture conditions are as follows: the temperature is 25-26 ℃, the light-dark ratio is 12h:12h, the pH is 7-9, and the illumination intensity is 86.4 mu mol (m)2·s)－1Obtaining the microalgae culture solution. The formula of BG11 culture medium is preferably NaNO3 1.5g/L，K2HPO4 0.04g/L，MgSO4·7H2O 0.075g/L，CaCl2·7H2O 0.036g/L，Na2CO30.02g/L, 0.006g/L citric acid, 0.006g/L ferric citrate, and 1mL/L trace element solution, wherein the trace element solution comprises: h3BO4 2.86g/L，MnCl2·4H2O 1.81g/L，ZnSO4 0.222g/L，Na2MoO4 0.39g/L，CuSO4·5H2O 0.079g/L，Co(NO3)26H2O 49.4g/L。
S2, growth phase (microbial biomass concentration 1X 10)∧6-1×10∧7cfu/mL) in a high-speed refrigerated centrifuge at 4 deg.C and 3500 r.min－1Centrifuging for 10min, discarding supernatant, and treating with 15 mg. L－1NaHCO of3The solution washes algae, removes nutrient salt adsorbed on the surface of algae cells, then washes the algae with sterile water, transfers the algae to a non-N culture medium to culture for 3d so as to exhaust the nutrient salt in the algae cells, and finally obtains the algae cells by centrifugation.
S3, mixing the concentrated solution of the algae cell with the sodium alginate and polyvinyl alcohol solution which are sterilized in advance according to the volume ratio of 1: 1, and stirringMixing to obtain a mixture, injecting into a 60mL syringe, adding into pre-cooled CaCl2–H3BO3And (3) dripping the mixed solution into a position 20cm away from the liquid level of the solution to form an algal ball with the diameter of 4mm, standing and fixing the algal ball in a refrigerator at the temperature of 4 ℃ for 24 hours, and preparing the immobilized microalgae beads 17. The preferable concentration of the sodium alginate solution is 3 percent, the preferable concentration of the polyvinyl alcohol is 8 percent, and CaCl is added2Preferably at a concentration of 2%, H3BO3The preferred concentration is 5.6%.
Example 4
In order to control parameters, the invention uses simulated wastewater to replace actual wastewater for testing, and the C/N in the wastewater is controlled to be 0.5. Wherein the ammonia nitrogen concentration is 40mg/L, the nitrate nitrogen concentration is 100mg/L, the nitrite nitrogen concentration is 100mg/L, the total nitrogen concentration is 240mg/L, the total phosphorus concentration is 15mg/L, and other conditions are kept unchanged.
Example 5
The C/N in the simulated wastewater is controlled to be 1. Wherein the ammonia nitrogen concentration is 40mg/L, the nitrate nitrogen concentration is 100mg/L, the nitrite nitrogen concentration is 100mg/L, the total nitrogen concentration is 240mg/L, the total phosphorus concentration is 15mg/L, and other conditions are kept unchanged.
Example 6
The C/N in the simulated wastewater is controlled to be 2. Wherein the ammonia nitrogen concentration is 40mg/L, the nitrate nitrogen concentration is 100mg/L, the nitrite nitrogen concentration is 100mg/L, the total nitrogen concentration is 240mg/L, the total phosphorus concentration is 15mg/L, and other conditions are kept unchanged.
Example 7
C/N in the simulated wastewater is controlled to be 3, wherein the ammonia nitrogen concentration is 40mg/L, the nitrate nitrogen concentration is 100mg/L, the nitrite nitrogen concentration is 100mg/L, the total nitrogen concentration is 240mg/L, the total phosphorus concentration is 15mg/L, and other conditions are kept unchanged.
Example 8
C/N in the simulated wastewater is controlled to be 4, wherein the ammonia nitrogen concentration is 40mg/L, the nitrate nitrogen concentration is 100mg/L, the nitrite nitrogen concentration is 100mg/L, the total nitrogen concentration is 240mg/L, the total phosphorus concentration is 15mg/L, and other conditions are kept unchanged.
TABLE 1 removal of contamination index in examples
As can be seen from Table 1, the two-phase immobilized microalgae and immobilized aerobic denitrifying bacteria bioreactor has a good effect on removing nitrogen and phosphorus elements in simulated wastewater, in example 4, when the C/N is controlled to be 0.5, the removal rates of total nitrogen and total phosphorus respectively reach 90.42% and 98.45, in examples 5 to 8, the C/N is continuously increased, the removal rate of total nitrogen increases with the increase of C/N, the removal rate of total phosphorus does not change greatly, and the low C/N resistant aerobic denitrifying bacteria has a good denitrification effect in low C/N wastewater.
The above-described embodiments are preferred implementations of the present invention, and the present invention may be implemented in other ways without departing from the spirit of the present invention.
Claims (10)
1. A two-phase immobilized bioreactor, comprising: including good oxygen denitrification reactor, immobilization little algae reactor and be used for the oxygen jar for good oxygen denitrification reactor oxygen suppliment, good oxygen denitrification reactor's liquid outlet and immobilization little algae reactor's inlet intercommunication, good oxygen denitrification reactor is provided with first immobilization carrier and waste water import, immobilization little algae reactor is provided with second immobilization carrier and waste water export.
2. The two-phase immobilized bioreactor of claim 1, wherein: be provided with first filtration baffle in the good oxygen denitrification reactor, first filtration baffle is used for separating the inner chamber of good oxygen denitrification reactor into the first immobilization room that is located first filtration baffle top and is located the filter chamber of first filtration baffle below, first immobilization carrier sets up in first immobilization indoor, the oxygen jar with cross worry room intercommunication, the waste water import sets up in the filter chamber.
3. The two-phase immobilized bioreactor of claim 2, wherein: an air outlet is formed in the top end of the aerobic denitrification reactor, and an liquid outlet of the aerobic denitrification reactor is formed in the side wall of the first immobilization chamber.
4. The two-phase immobilized bioreactor of claim 1, wherein: a second filtering baffle is arranged in the immobilized microalgae reactor and is used for dividing the inner cavity of the immobilized microalgae reactor into a second immobilization chamber positioned above the second filtering baffle and a liquid outlet chamber positioned below the second filtering baffle, the second immobilized carrier is arranged in the second immobilization chamber, the oxygen tank is communicated with the liquid outlet chamber, and the wastewater outlet is arranged in the liquid outlet chamber; the liquid inlet of the immobilized microalgae reactor is arranged on the side wall of the second immobilization chamber.
5. The two-phase immobilized bioreactor of claim 5, wherein: the immobilized microalgae reactor is provided with a stirrer and a light source for providing illumination, and the lower end of the stirrer extends into the second immobilized chamber.
6. The two-phase immobilized bioreactor of claim 1, wherein: the aerobic denitrification reactor and the immobilized microalgae reactor are both provided with a pH meter, a liquid level sensor and a dissolved oxygen meter.
7. The two-phase immobilized bioreactor of claim 1, wherein: the aerobic denitrification reactor and the immobilized microalgae reactor are both provided with thermometers and heating rods which are respectively used for heating liquid in the aerobic denitrification reactor and the immobilized microalgae reactor.
8. A method for treating black smelly water is characterized in that: the method for treating black odorous water adopts the two-phase type immobilized bioreactor as claimed in any one of claims 1 to 7, and comprises the following steps:
(1) conveying the wastewater to be treated into an aerobic denitrification reactor from a wastewater inlet, treating the wastewater by aerobic denitrifying bacteria fixed on a first immobilized carrier, and then feeding the wastewater into an immobilized microalgae reactor; the oxygen tank provides oxygen required by the aerobic denitrifying bacteria;
(2) after the wastewater treated by the aerobic denitrification reactor enters the immobilized microalgae reactor, treating the wastewater by microalgae arranged on a second immobilized carrier; the treated wastewater is discharged from a wastewater outlet.
9. An immobilization method of aerobic denitrifying bacteria, which is characterized in that: the method comprises the following steps:
A. preparing a polyurethane foam carrier;
B. polyurethane foam is adopted to immobilize aerobic denitrifying bacteria.
10. A microalgae immobilization method is characterized in that: the method comprises the following steps:
s1, inoculating the scenedesmus obliquus and chlorella vulgaris seed liquid to a culture medium for culture under the aseptic operation condition to obtain algae liquid;
s2, centrifuging the algae liquid in the logarithmic growth phase in a refrigerated centrifuge, discarding supernatant, washing algae bodies with sodium bicarbonate solution, removing nutritive salt adsorbed on the surfaces of algae cells, washing with sterile water, transferring the algae bodies to a nitrogen-free culture medium for culture to exhaust the nutritive salt in the algae cells, and finally centrifuging to obtain an algae cell concentrated solution;
s3, mixing the algae cell concentrated solution with sodium alginate and polyvinyl alcohol solution which are sterilized in advance, and uniformly stirring to form mixed solution; to CaCl2–H3BO3And dripping the mixed solution on the solution surface to form the microalgae spheres, and standing and fixing in a refrigerator at the temperature of 2-6 ℃ to obtain the immobilized microalgae spheres.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113003727A (en) * | 2021-03-02 | 2021-06-22 | 重庆理工大学 | Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof |
| CN114671577A (en) * | 2022-05-30 | 2022-06-28 | 无棣永利盐业有限公司 | Denitrification purification method for mariculture wastewater |
| WO2022219950A1 (en) * | 2021-04-15 | 2022-10-20 | 日清紡ケミカル株式会社 | Microorganism-immobilized carrier for water treatment, flexible polyurethane foam composition, and method for producing flexible polyurethane foam |
| CN117821322A (en) * | 2023-12-29 | 2024-04-05 | 深圳市欧达尔环保科技有限公司 | Culture method of nitrifying bacteria |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007035707A1 (en) * | 2007-07-30 | 2009-02-05 | GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V. | Method for oxygenization and pollutant absorption in aquatic systems, comprises cultivating micro algae in photo bioreactors, separating and concentrating the cells, immobilizing concentrate in polymer carriers, and collecting aggregates |
| CN103964640A (en) * | 2014-05-14 | 2014-08-06 | 中国环境科学研究院 | Integral denitrification combination device and denitrification method |
| CN104498469A (en) * | 2014-12-09 | 2015-04-08 | 陕西科技大学 | Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage |
| CN108439591A (en) * | 2018-03-14 | 2018-08-24 | 中国市政工程中南设计研究总院有限公司 | A kind of separate type solidification phycomycete sewage-treatment plant and method |
| CN108585195A (en) * | 2018-03-14 | 2018-09-28 | 重庆理工大学 | Low temperature low-carbon ammonia nitrogen wastewater treatment device and method based on solid phase denitrification and heterotrophic nitrification-aerobic denitrification |
| CN210656587U (en) * | 2019-08-23 | 2020-06-02 | 广东中微环保生物科技有限公司 | Reactor for immobilized microalgae-bacteria co-treatment of sewage |
-
2020
- 2020-10-22 CN CN202011140225.9A patent/CN112174325A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007035707A1 (en) * | 2007-07-30 | 2009-02-05 | GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V. | Method for oxygenization and pollutant absorption in aquatic systems, comprises cultivating micro algae in photo bioreactors, separating and concentrating the cells, immobilizing concentrate in polymer carriers, and collecting aggregates |
| CN103964640A (en) * | 2014-05-14 | 2014-08-06 | 中国环境科学研究院 | Integral denitrification combination device and denitrification method |
| CN104498469A (en) * | 2014-12-09 | 2015-04-08 | 陕西科技大学 | Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage |
| CN108439591A (en) * | 2018-03-14 | 2018-08-24 | 中国市政工程中南设计研究总院有限公司 | A kind of separate type solidification phycomycete sewage-treatment plant and method |
| CN108585195A (en) * | 2018-03-14 | 2018-09-28 | 重庆理工大学 | Low temperature low-carbon ammonia nitrogen wastewater treatment device and method based on solid phase denitrification and heterotrophic nitrification-aerobic denitrification |
| CN210656587U (en) * | 2019-08-23 | 2020-06-02 | 广东中微环保生物科技有限公司 | Reactor for immobilized microalgae-bacteria co-treatment of sewage |
Non-Patent Citations (1)
| Title |
|---|
| 庞长泷等: "低温好氧反硝化菌群强化生活污水脱氮效能", 《哈尔滨工业大学学报》 * |
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| CN113003727A (en) * | 2021-03-02 | 2021-06-22 | 重庆理工大学 | Bacteria and algae composition for synergistically degrading high-concentration ammonia nitrogen and phosphate and application and method thereof |
| WO2022219950A1 (en) * | 2021-04-15 | 2022-10-20 | 日清紡ケミカル株式会社 | Microorganism-immobilized carrier for water treatment, flexible polyurethane foam composition, and method for producing flexible polyurethane foam |
| CN114671577A (en) * | 2022-05-30 | 2022-06-28 | 无棣永利盐业有限公司 | Denitrification purification method for mariculture wastewater |
| CN114671577B (en) * | 2022-05-30 | 2022-08-26 | 无棣永利盐业有限公司 | Denitrification purification method for mariculture wastewater |
| CN117821322A (en) * | 2023-12-29 | 2024-04-05 | 深圳市欧达尔环保科技有限公司 | Culture method of nitrifying bacteria |
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