CN112206286A - Application of black highland barley and black quinoa composition in preparation of antioxidant product - Google Patents
Application of black highland barley and black quinoa composition in preparation of antioxidant product Download PDFInfo
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Abstract
The invention discloses application of a highland barley and quinoa composition in preparing an antioxidant product, wherein the composition comprises the following components in parts by weight: 3-24 parts of highland barley alcohol extract, 2-16 parts of highland barley water extract, 4-10 parts of quinoa alcohol extract and 5-7 parts of quinoa water extract. The composition has high biological activity and antioxidant activity.
Description
Technical Field
The invention belongs to the field of medicines, and particularly relates to application of a black highland barley and black quinoa composition in preparing an antioxidant product.
Background
With the improvement of living standard, people pay more attention to skin problems caused by oxidation, such as skin aging, inflammation and even cancer. Aging and damage of the skin, which are important characteristics of aging of the human body due to direct contact with oxygen, are caused by the direct contact of the skin with oxygen, and the aging of the human body is caused by the accumulation or removal of reactive oxygen species free radicals, which cause cell damage and pigmentation.
Free radicals generated by the organism in metabolism are most harmful to the organism as oxygen radicals, including superoxide anion free radicals, hydroxyl free radicals, hydrogen peroxide, singlet oxygen and the like, which are normal metabolites of the organism, and although they are continuously generated in metabolism, the radicals are not harmful under normal conditions due to the existence of a defense system in the organism, and the superoxide anion free radicals therein can be eliminated by superoxide dismutase (SOD). SOD is an important oxygen free radical scavenger in vivo, and can balance oxygen free radicals of organism, thereby avoiding adverse reaction caused by excessive concentration of superoxide anion free radicals in vivo. Although the human body has antioxidant enzymes and non-enzymatic systems to resist oxidative damage, if the human body is subjected to oxidative damage of excessive Reactive Oxygen Species (ROS), the protective system of the human body is not enough to remove the ROS, and an antioxidant is required. However, long-term use of antioxidants can produce toxic effects. Thus, more natural and safer antioxidants and anti-aging drugs are increasingly sought after.
Highland barley (Hordeum vulgare ssp. vulgare) is a crop growing in high-altitude low-temperature low-oxygen environment, also called naked barley, and is a barley crop of Gramineae. There has been thousands of years of history as a major food crop in many countries and regions of the world. The highland barley is classified into white highland barley, black highland barley, dark green highland barley and the like, wherein the black highland barley is a specific variety in Tibet, the black highland barley is produced in prefecture county in southern Shane of Himalayan mountain due to special climate and soil, the fat, crude fiber and anthocyanin content of the highland barley are high, the highland barley has a good health care function, and the highland barley is deeply favored by food processing enterprises and markets.
Chenopodium quinoa willd, also called as Nanmei quinoa, and Jinguzi, etc., native to America Andes mountain area, is the main traditional food for printed and indigenous residents, due to its unique abundant and comprehensive nutritive value, nourishes printed and indigenous people, called as food mother, and can also replace infant milk powder for eating. It is rich in protein, dietary fiber and mineral, has balanced amino acid composition, and is rich in phenolic compounds such as phenolic acid, polyphenol and flavone. Wherein the phenolic compounds ingested into the human body have physiological effects of antioxidation, anti-inflammation, antivirus and anticancer. The quinoa belongs to quinoa, has mild nature and sweet taste, contains rich protein, carbohydrate, fat and various amino acids, contains all 9 amino acids necessary for human bodies, is suitable in proportion and easy to absorb, is also rich in unsaturated fatty acid, anthocyanin, flavonoid, beta-glucan, choline, betaine, alpha-linolenic acid, various mineral elements, vitamin B group, folic acid, vitamin E and other compounds, has the dietary fiber content of up to 7.1 percent, does not contain cholesterol, and has the effects of repairing the physique, improving the body adaptability, regulating the internal secretion, preventing diseases, beautifying, losing weight, relaxing the bowels, delaying senescence, preventing and resisting cancers, lowering blood pressure, blood fat and blood sugar, preventing and treating cardiovascular and cerebrovascular diseases, assisting in treating chronic diseases and the like.
Disclosure of Invention
The invention aims to provide the application of the highland barley and quinoa composition in preparing an antioxidant product, the biological activity is high, the content of superoxide dismutase (SOD) can be increased, the content of Malondialdehyde (MDA) can be reduced, and the antioxidant activity is realized.
In order to achieve the purpose, the invention provides the following technical scheme:
the use of a composition in the preparation of an antioxidant product, the composition comprising the following components in parts by weight: 3-24 parts of highland barley alcohol extract, 2-16 parts of highland barley water extract, 3-10 parts of quinoa alcohol extract and 5-7 parts of quinoa water extract.
In the specific embodiment of the invention, the highland barley alcohol extract: the mass ratio of the highland barley aqueous extract to the highland barley aqueous extract is 1.5-2.4: 1, preferably 1.5:1, and the weight ratio of the chenopodium quinoa alcoholic extract is as follows: the mass ratio of the black quinoa water extract is 0.8-2: 1, and preferably 0.8: 1.
In the specific embodiment of the invention, the composition comprises 12-24 parts of highland barley alcohol extract, 8-16 parts of highland barley water extract, 4-7 parts of quinoa alcohol extract and 5 parts of quinoa water extract.
Further, the composition comprises 12 parts of highland barley alcohol extract, 8 parts of highland barley water extract, 4 parts of quinoa alcohol extract and 5 parts of quinoa water extract.
In a specific embodiment of the present invention, the alcohol of the alcohol extract is selected from one or more of methanol, ethanol, butanol, pentanol.
Further, the alcohol is selected from ethanol; further, the aqueous solution of 20 to 100% v/v ethanol, preferably 35 to 95% v/v ethanol.
In a particular embodiment of the invention, the product is a superoxide dismutase agonist and/or a product that reduces the level of malondialdehyde in the body.
The MDA content is an important parameter reflecting the potential oxidation resistance of an organism, can reflect the lipid peroxidation rate and strength of the organism, and can also indirectly reflect the tissue peroxidation damage degree.
Further, the product is a product for eliminating oxygen free radicals in vivo, and the product is a food, a health product, a medicine or a cosmetic.
The invention also provides an antioxidant product, which is prepared by taking the highland barley alcohol extract, the highland barley water extract, the quinoa alcohol extract and the quinoa water extract as active ingredients;
the black highland barley and black quinoa health-care beverage comprises 3-24 parts of black highland barley alcohol extract, 2-16 parts of black highland barley water extract, 3-10 parts of black quinoa alcohol extract and 5-7 parts of black quinoa water extract.
In the specific embodiment of the invention, the black highland barley alcohol extract comprises 12 parts of black highland barley alcohol extract, 8 parts of black highland barley water extract, 4 parts of black chenopodium quinoa alcohol extract and 5 parts of black chenopodium quinoa water extract.
The superoxide dismutase stimulant is a product which can only increase the content of superoxide dismutase (SOD) in organisms.
In vivo, free radicals act on lipid to generate peroxidation, and the oxidation end product is malondialdehyde, which can cause cross-linking polymerization of life macromolecules such as protein and nucleic acid, and has cytotoxicity. The composition can reduce the content of Malondialdehyde (MDA) in mouse serum, which shows that the composition can reduce the occurrence of lipid peroxidation and has antioxidation effect.
The invention has the following beneficial effects:
(1) the extraction method provided by the invention has simple steps, is simple, convenient and quick, can effectively reduce the cost of related products of the black highland barley and the black quinoa, and is worthy of popularization.
(2) The invention provides a series of compositions consisting of highland barley aqueous extract, highland barley alcohol extract, quinoa aqueous extract and quinoa alcohol extract, and tests prove that the compositions can up-regulate SOD content and down-regulate MDA content, have antioxidant activity and can be used for preparing products with antioxidant effect.
Detailed Description
The technical solutions of the present invention are described clearly and completely below, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art without inventive efforts based on the embodiments of the present invention, belong to the scope of the present invention.
Example 1
Preparation of highland barley extract and quinoa extract
(1) Preparing the highland barley alcohol extract: taking dry highland barley powder which is dried to constant weight, respectively adding 95% v/v, 85% v/v, 75% v/v, 65% v/v, 55% v/v, 45% v/v and 35% v/v ethanol for reflux extraction for 7 times, filtering, reserving medicine residues for later use, combining extracting solutions, recovering ethanol, drying to obtain highland barley alcohol extract, and determining that the paste yield is about 29.93%.
(2) Preparing a highland barley aqueous extract: and (2) taking the medicine residues in the step (1), adding water, decocting for 5 times, filtering, combining the filtrates, concentrating and drying to obtain the highland barley water extract, and determining that the cream yield is about 20.18%.
(3) Preparing the black quinoa alcohol extract: and (3) respectively adding 95% v/v, 85% v/v, 75% v/v, 65% v/v, 55% v/v, 45% v/v and 35% v/v ethanol into the dried black chenopodium quinoa powder which is dried to constant weight, reflux-extracting for 7 times, filtering, reserving medicine residues for later use, combining extracting solutions, recovering ethanol, and drying to obtain the black chenopodium quinoa ethanol extract, wherein the paste yield is about 21.38% by determination.
(4) Preparing a black quinoa water extract: and (2) taking the medicine residues in the step (1), adding water, decocting for 5 times, filtering, combining the filtrates, concentrating and drying to obtain the black quinoa water extract, and determining that the cream yield is about 15.37%.
The above-mentioned cream yield can be normally fluctuated within the range of 5%.
Example 2
And (3) uniformly mixing 12 parts of the highland barley alcohol extract, 8 parts of the highland barley water extract, 4 parts of the quinoa alcohol extract and 5 parts of the quinoa water extract, which are prepared in the embodiment 1, so as to obtain the composition.
Example 3
Taking 12 parts of the highland barley alcohol extract, 8 parts of the highland barley water extract, 4 parts of the quinoa alcohol extract and 5 parts of the quinoa water extract prepared in the embodiment 1, adding a proper amount of microcrystalline cellulose, uniformly mixing, drying and encapsulating to obtain the capsule of the composition.
Example 4 detection of oxidase content
10 male ICR mice with the weight of 20-22g are taken, and are randomly grouped into a normal control group, an aging model group, a vitamin E group (positive control group) and different compatible medicine groups for verifying the antioxidant effect of the extract. Normal control group injected the same volume of normal saline into mice per day subcutaneously, and the other groups injected D-galactose into the mice 150mg (/ kg. D) continuously for 55D subcutaneously, to prepare the mouse subacute peroxidation injury model, and the behavioral detection shows that the model building is successful. The administration is started by intragastric administration of 150g (/ kg. D) the next day after the first injection of D-galactose, the experimental group is intragastric administered with the highland barley black and the quinoa black extract groups with different proportions according to the corresponding dose, the positive control group is replaced by 50mg (/ kg. D) vitamin E, and the normal control group and the aging model group are administered with drinking water. Each group of mice was housed in the same room and fed freely. After the last administration, fasting was carried out for 24h, the eyeball was removed and blood was taken, and the mice were killed by removing the neck. Centrifuging the plasma at 4 deg.C and 3500r/min for 10min, separating serum, and determining SOD level and MDA level according to kit instructions.
TABLE 1 influence of the extract of Avena nigra and the extract of Chenopodium nigra on the oxidation indexes SOD and MDA in the serum of mice
As can be seen from the data in table 1, when the highland barley aqueous extract and the black quinoa aqueous extract are used alone, although the content of Malondialdehyde (MDA) is reduced, the content of superoxide dismutase (SOD) is reduced, and when the highland barley alcoholic extract and the black quinoa alcoholic extract are used alone, the content of SOD can be increased, the content of MDA can be reduced, but the effect is weak; after the highland barley alcohol extract, the highland barley water extract, the chenopodium quinoa alcohol extract and the chenopodium quinoa water extract are combined, the content of SOD in serum of a mouse is higher than the using effect of any single extract or every two extracts, and the content of MDA is lower than the using effect of any single extract or every two extracts, so that the synergistic effect of the highland barley alcohol extract, the highland barley water extract, the chenopodium quinoa alcohol extract and the chenopodium quinoa water extract is shown after the compatibility, the antioxidant activity of the extracts is improved, wherein the highland barley alcohol extract: the highland barley water extract comprises the following components: black chenopodium quinoa willd alcohol extract: the anti-oxidation effect is strongest when the black chenopodium quinoa aqueous extract is 12:8:4: 5.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all changes in equivalent flow or equivalent structure, which are made by using the description of the present invention and are directly or indirectly applied to other related technical fields should be covered by the scope of the present invention.
Claims (10)
1. The application of the composition in preparing an antioxidant product is characterized in that the composition comprises the following components in parts by weight: 3-24 parts of highland barley alcohol extract, 2-16 parts of highland barley water extract, 3-10 parts of quinoa alcohol extract and 5-7 parts of quinoa water extract.
2. The use of claim 1, wherein the highland barley alcohol extract: the mass ratio of the highland barley aqueous extract to the highland barley aqueous extract is 1.5-2.4: 1, preferably 1.5:1, and the weight ratio of the chenopodium quinoa alcoholic extract is as follows: the mass ratio of the black quinoa water extract is 0.8-2: 1, and preferably 0.8: 1.
3. The use of claim 1, wherein the composition comprises 12-24 parts of highland barley alcohol extract, 8-16 parts of highland barley water extract, 4-7 parts of quinoa alcohol extract and 5 parts of quinoa water extract.
4. The use of claim 3, wherein the composition comprises 12 parts of highland barley alcohol extract, 8 parts of highland barley water extract, 4 parts of quinoa alcohol extract and 5 parts of quinoa water extract.
5. The use as claimed in any one of claims 1 to 4, wherein the alcohol of the alcohol extract is selected from one or more of methanol, ethanol, butanol and pentanol.
6. Use according to claim 5, characterized in that the alcohol is selected from ethanol; further, the aqueous solution of 20 to 100% v/v ethanol, preferably 35 to 95% v/v ethanol.
7. Use according to any one of claims 1 to 6, wherein the product is a superoxide dismutase agonist and/or a product which reduces the amount of malondialdehyde in vivo.
8. Use according to claim 7, wherein the product is a product for scavenging oxygen free radicals in the body.
9. An antioxidant product is characterized in that the product is prepared by taking highland barley alcohol extract, highland barley water extract, quinoa alcohol extract and quinoa water extract as active ingredients;
the black highland barley and black quinoa health-care beverage comprises 3-24 parts of black highland barley alcohol extract, 2-16 parts of black highland barley water extract, 3-10 parts of black quinoa alcohol extract and 5-7 parts of black quinoa water extract.
10. The product of claim 9, comprising 12 parts of highland barley alcohol extract, 8 parts of highland barley water extract, 4 parts of quinoa alcohol extract and 5 parts of quinoa water extract.
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| CN111329049A (en) * | 2020-03-30 | 2020-06-26 | 江南大学 | Preparation method of quinoa and highland barley enzyme powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111329049A (en) * | 2020-03-30 | 2020-06-26 | 江南大学 | Preparation method of quinoa and highland barley enzyme powder |
Non-Patent Citations (2)
| Title |
|---|
| 华艳宏等: "藜麦种子不同溶剂提取物及其抗氧化活性", 《江苏农业科学》 * |
| 申迎宾等: "提取溶剂对青稞提取物总酚、黄酮含量及其抗氧化活性的影响", 《食品与机械》 * |
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