CN114748380B - Composition containing marine plant extract and application thereof - Google Patents
Composition containing marine plant extract and application thereof Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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Abstract
The invention discloses a composition containing marine plant extracts and application thereof, wherein the composition containing the marine plant extracts comprises the following components in parts by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract. By compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract in a specific proportion, the antibacterial performance and the oxidation resistance can be obviously improved, the rapid and long-acting antibacterial effect can be achieved, and the Chinese medicinal preparation has the advantages of low dosage, safety, no stimulation and the like when being used in cosmetics.
Description
Technical Field
The invention belongs to the field of bacteriostasis, and particularly relates to a composition containing marine plant extracts and application thereof.
Background
The marine plant resources in China are rich, various animals, plants and microorganisms exist in the ocean, the marine organisms have different metabolic pathways from terrestrial organisms due to special environments such as ocean high pressure, high salt and the like, and many marine plant components have specific chemical structures and wide pharmacological activities such as halogenated terpenes, polyphenols, neurotoxin, polysaccharide, polypeptides and the like, and the main pharmacological effects comprise antibiosis, anti-inflammation, antioxidation, anti-tumor, antivirus, cardiovascular disease treatment, immunoregulation function and the like, and most of the substances have good effects of antibiosis, antivirus, anti-inflammatory, antioxidation, antitumor, cardiovascular disease treatment, immunoregulation function and the like, so that the clinical development application prospect is wide.
The marine plant has rich nutritive value, and has the following nutritional characteristics: 1. the crude protein has high content, contains abundant multiple vitamins and minerals, and is favorable for breeding, growing and developing livestock and poultry. 2. Contains antibacterial active substances. The active substances have inhibiting effect on mold, staphylococcus aureus, escherichia coli, salmonella and the like, and can improve the immunity and disease resistance of livestock and poultry. 3. Contains bioactive hormone and growth promoting factor. Can regulate balance of various nutrients in feed, promote digestion and absorption of nutrients, exert anti-stress effect, and improve growth speed and disease resistance of livestock and fowl. 4. Contains abundant pigment. Marine plants contain substances such as phycocyanin, carotene and the like; can be used in livestock and fowl feed to obviously improve quality of livestock and fowl products and make skin and muscle of livestock and fowl have bright color. 5. High iodine content and moderate calcium and phosphorus ratio. The livestock and poultry feed prepared by using the feed can enrich the nutrition of meat, eggs and milk products and has delicious taste; the thickness of the eggshell is increased, and the color of the yolk is changed into dark yellow; in particular, the iodine content in the yolk is ten times higher than the original iodine content; the fat structure is also changed, so that the content of cholesterol is reduced. 6. Contains phenol compounds, airing glue, alginic acid and other substances. The feed mildew-proof agent contains phenol compounds, has strong antibacterial and mildew-proof effects, is a natural feed mildew-proof agent, contains agar, alginic acid and water-absorbing substances, is a natural feed adhesive and a moisture-proof agent, and can absorb moisture in feed. 7. The sulfuric acid ester obtained by iodizing brown algae starch can replace heparin and has the functions of reducing blood fat, resisting blood coagulation and improving microcirculation system.
Sargassum fusiforme is a marine plant, belongs to algae, and is mainly distributed in Zhejiang, fujian and other places in China. The Sargassum fusiforme extract contains rich polysaccharide, dietary cellulose, B vitamins, alginic acid, and mineral substances and microelements necessary for human body, and has good bioactivity but poor antibacterial and antioxidant properties. Therefore, the improvement of the antibacterial property and the oxidation resistance of the sargassum fusiforme extract is of great significance.
Disclosure of Invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a marine plant extract-containing composition comprising the following mass contents: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract. By compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract in a specific proportion, the components have a synergistic effect, the antibacterial performance and the oxidation resistance can be obviously improved, and the rapid and long-acting antibacterial effect can be achieved. The composition containing marine plant extract can be used in cosmetics, and has the advantages of low dosage, safety, no irritation, etc.
The invention aims to provide a composition containing marine plant extracts, which comprises the following components in percentage by mass: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract.
Sargassum fusiforme is a marine plant, and belongs to algae, such as deer horn tip, seaweed bud, sheep milk, sea barley, and the like. Oncomelania, sargassaceae. The sargassum fusiforme has rich nutrition. The fresh sargassum fusiforme has water content of about 83.16%, and contains mainly protein, carbohydrate, ash, fiber and the like in dry basis, which respectively account for 15%, 46%, 30% and 7%, and is a natural food with low fat and heat, and has high nutritive value, and can be used as vegetable or as medicine. The Sargassum fusiforme extract contains rich polysaccharide, dietary cellulose, B vitamins, alginic acid, and essential minerals and microelements, and has effects of promoting cell growth and development, inhibiting bacteria and resisting oxidation.
Centella asiatica, perennial herbs, stolons, long and thin, and root on knots. Centella asiatica extract contains asiatic acid and madecassic acid, has an inhibitory effect on staphylococcus aureus, and has an obvious antioxidation effect.
Herba Artemisiae Scopariae is a herb of Artemisia of Compositae, and has strong fragrance. The herba Artemisiae Scopariae extract contains volatile oil component, vitamins, minerals and terpenoids, has antiinflammatory, antibacterial and antioxidant effects, and can inhibit various free radical activities in skin cells, relieve skin aging, and restore skin vigor and antiinflammatory, antibacterial and skin relieving effects.
The black soybean is mature seed of leguminous plants, and is the main raw material of traditional Chinese medicine fermented soybean. The black soybean extract contains soybean isoflavone, anthocyanin, tannin and other bioactive components, and has various bioactivity such as relieving climacteric syndrome, resisting oxidation, protecting cardiovascular system, resisting cancer, resisting bacteria and resisting virus.
The inventor finds that the composition prepared by compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract in a specific proportion has synergistic antibacterial and antioxidant effects among the components, and probably because the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract all contain hydroxyl compounds, hydrogen bonding effects exist among the hydroxyl compounds, and all active components coordinate and promote each other, so that the composition has good synergistic effects.
Preferably, the marine plant extract-containing composition comprises the following mass contents: 20% of sargassum fusiforme extract, 10% of centella asiatica extract, 10% of artemisia capillaris extract and 20% of black soybean extract.
Preferably, the extraction process of the sargassum fusiforme extract is as follows:
reflux extracting dried and pulverized Cyrtymenia Sparsa with solvent, centrifuging, and concentrating supernatant to obtain Cyrtymenia Sparsa extract.
Preferably, the solvent is selected from 95% ethanol.
Preferably, the extraction process of the centella asiatica extract is as follows:
mixing dried herba Centellae with deionized water, soaking, heating to boil, separating medicinal liquid, adding deionized water into the residue, heating to boil, mixing the medicinal liquids, filtering, centrifuging, and concentrating the supernatant to obtain herba Centellae extract.
Preferably, the extraction process of the artemisia capillaris extract is as follows:
mixing the dried and sieved herba Artemisiae Scopariae powder with the extractive solution, leaching, ultrasonic extracting, decolorizing, filtering, and concentrating to obtain herba Artemisiae Scopariae extract.
Preferably, the extract is selected from water and ethanol mixed solvents with a volume ratio of 1:9; the leaching temperature is 60-70 ℃.
Preferably, the extraction process of the black soybean extract is as follows:
mixing the dried and sieved black soybean powder with the extract, leaching, regulating the pH value, performing ultrasonic extraction, filtering, decoloring, filtering and concentrating the filtrate to obtain the black soybean extract.
Preferably, the extract is selected from a mixed solvent of water and butanediol in a volume ratio of 2:8; the leaching temperature is 40-50 ℃; the pH value is 4.0-5.0.
Preferably, the marine plant extract-containing composition comprises 10-70% by mass of the solvent.
Preferably, the solvent is selected from one or more of ethanol, 1, 2-propanediol, 1, 3-propanediol, 1, 2-butanediol, 1, 3-butanediol, 1, 4-butanediol, 1, 2-pentanediol, 1, 5-pentanediol, isopentane diol, 1, 2-hexanediol, 1, 6-hexanediol, water, glycerol.
Another object of the present invention is to provide a method for preparing a composition containing marine plant extracts, comprising the steps of:
mixing Cyrtymenia Sparsa extract 10-30%, herba Centellae extract 5-15%, herba Artemisiae Scopariae extract 5-15%, semen Sojae Atricolor extract 10-30% and solvent 10-70%, stirring, heating to 45-55deg.C, cooling, and packaging.
It is another object of the present invention to provide the use of the above marine plant extract-containing composition for the preparation of a cosmetic containing the above marine plant extract-containing composition.
Preferably, the cosmetic is selected from any one of facial mask liquid, emulsion, cream, essence, stock solution, face cleansing cream, foundation solution, foundation cream and concealer.
Preferably, the marine plant extract-containing composition is 0.1-0.5% by mass in the cosmetic.
Preferably, the marine plant extract-containing composition is 0.5% by mass in the cosmetic.
Compared with the prior art, the invention has the following beneficial effects:
(1) The components of the composition containing the marine plant extract are all derived from natural plants, are safe and non-irritating, and have good synergistic effect due to mutual coordination and mutual promotion of active components.
(2) According to the invention, through compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract in a specific proportion, the antibacterial performance and the oxidation resistance of the composition can be remarkably improved, and the rapid and long-acting antibacterial effect can be achieved.
(3) The composition containing the marine plant extract can achieve high-efficiency antibacterial and antioxidant effects under lower dosage.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1: extracting Cyrtymenia Sparsa.
Pulverizing dried Cyrtymenia Sparsa 100kg, sieving with 50 mesh sieve, leaching with 500mL 95% ethanol at 78deg.C for 2-3 hr, centrifuging for 10min, filtering to remove insoluble substances, decolorizing the filtrate with active carbon, filtering to remove active carbon, centrifuging, and concentrating the supernatant to obtain Cyrtymenia Sparsa extract.
Example 2: extracting centella asiatica.
Pulverizing dried herba Centellae 100kg, sieving with 100 mesh sieve, adding 500mL deionized water, soaking for 10 hr, heating to boil, separating medicinal liquid, adding 500mL deionized water into the residue, heating to boil, mixing the medicinal liquids after boiling twice, filtering to remove insoluble substances, decolorizing the filtrate with active carbon, filtering to remove active carbon, centrifuging, concentrating the supernatant to obtain herba Centellae extract.
Example 3: extracting herba Artemisiae Scopariae.
Pulverizing dried herba Artemisiae Scopariae 100kg, sieving with 50 mesh sieve, adding 1000mL of mixed solvent of water and ethanol at volume ratio of 1:9, leaching at 60-70deg.C for 3-5 hr, ultrasonic extracting, decolorizing the filtrate with active carbon, filtering to remove active carbon, and concentrating the filtrate to obtain herba Artemisiae Scopariae extract.
Example 4: extraction of black soybeans.
Pulverizing dried semen Sojae Atricolor 100kg, sieving with 50 mesh sieve, adding 1000mL of mixed solvent of water and butanediol with volume ratio of 2:8, leaching at 40-50deg.C for 3-4 hr, adjusting pH to 4.0-5.0, ultrasonic extracting, filtering, decolorizing the filtrate with active carbon, filtering, and concentrating the filtrate to obtain semen Sojae Atricolor extract.
Example 5: preparation of a composition containing marine plant extract.
Mixing Cyrtymenia Sparsa extract 20g, herba Centellae extract 10g, herba Artemisiae Scopariae extract 10g, semen Sojae Atricolor extract 20g and 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Example 6: preparation of a composition containing marine plant extract.
Mixing Cyrtymenia Sparsa extract 10g, herba Centellae extract 15g, herba Artemisiae Scopariae extract 5g, semen Sojae Atricolor extract 30g and 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Example 7: preparation of a composition containing marine plant extract.
Mixing Cyrtymenia Sparsa extract 30g, herba Centellae extract 5g, herba Artemisiae Scopariae extract 15g, semen Sojae Atricolor extract 10g and 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain composition containing marine plant extract.
Comparative example 1: preparation of one-component compositions.
Mixing Cyrtymenia Sparsa extract 60g with 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single-component composition.
Comparative example 2: preparation of one-component compositions.
Mixing herba Centellae extract 60g with 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single component composition.
Comparative example 3: preparation of one-component compositions.
Mixing herba Artemisiae Scopariae extract 60g with 1, 3-butanediol 40g, stirring, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single component composition.
Comparative example 4: preparation of one-component compositions.
Mixing and stirring 60g of black soybean extract and 40g of 1, 3-butanediol, heating to 50deg.C, stirring for dissolving, cooling to room temperature, and filtering to remove insoluble impurities to obtain single-component composition.
Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC):
preparation of liquid culture medium: weighing 10g of peptone, 10g of glucose, 5g of sodium chloride and 5g of beef extract, adding water, boiling to fully dissolve each component, fixing the volume to 1000mL, cooling to normal temperature, regulating the pH to be neutral, sterilizing the culture medium under the conditions of 0.1MPa and 120 ℃ for 30min, cooling to normal temperature, and storing in a refrigerator at 4 ℃ for later use.
(2) Preparation of bacterial suspension:
and (3) strain: staphylococcus aureus ATCC6538 was from the collection of microorganisms from the cantonese province.
Preparation of bacterial suspension: inoculating Staphylococcus aureus in logarithmic phase in liquid culture medium, culturing at 37deg.C for 12 hr, preparing bacterial liquid into bacterial suspension with 0.5 Maillard turbidity with sterile liquid culture medium, and diluting at a ratio of 1:1000 to obtain bacterial content of about 10 5 CFU/mL。
(3) Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC).
The proper bacteriostasis and sterilization concentration range is searched by adopting a double dilution method: staphylococcus aureus in logarithmic growth phase was inoculated into liquid medium (bacterial suspension concentration 1×10) 5 CFU/mL), the compositions of examples 5-7 and comparative examples 1-4 were added in equal amounts at different concentrations, so that the concentrations of the compositions of examples 5-7 and comparative examples 1-4 in the medium were: 160. 80, 40, 20, 10, 5 and 2.5mg/mL, shake culturing at 37deg.C and 120r/min for 24 hr, observing growth condition, and recording the lowest concentration without bacterial growth in the test tube to determine suitable antibacterial concentration range.
Determining the minimum inhibitory concentration and the minimum bactericidal concentration by adopting a gradient method: staphylococcus aureus in logarithmic growth phase was inoculated into liquid medium (bacterial suspension concentration 1×10) 5 CFU/mL), the compositions of examples 5-7, comparative examples 1-4 were added in equal amounts at different concentrations, respectively, to give the compositions of examples 5-7, comparative examples 1-4 in the medium at the following concentrations: the concentration determined by the double dilution method is tested according to the concentration gradient of 0.5mg/mL,
meanwhile, sterile water and 1, 3-butanediol are respectively used as blank control groups, and observed after shake culture is carried out for 24 hours at 37 ℃ and 120r/min, the minimum dilution concentration of the clear color of the liquid culture medium is the minimum sterilization concentration (MIC). And coating the bacterial liquid on a flat plate, and observing the growth condition of the bacterial body, wherein the minimum dilution concentration of the bacterial body which does not grow is the MBC value.
Table 1 MIC and MBC measurements for the compositions of examples 5-7 and comparative examples 1-4.
As can be seen from Table 1, the composition containing marine plant extract prepared by compounding the Sargassum fusiforme extract, the centella asiatica extract, the herba Artemisiae Scopariae extract and the black soybean extract has synergistic antibacterial effect.
Determination of DPPH radical scavenging Rate:
40mg of DPPH was weighed, 1000mL of methanol was added for dissolution, and 0.04mg/mL of DPPH methanol solution was obtained, and sample solutions having a concentration of 0.5mg/mL were prepared in examples 5 to 7 and comparative examples 1 to 4, respectively. 2mL of DPPH methanol solution was added to 2mL of each sample solution, the reaction was carried out in the dark for 30min, and the absorbance at 517nm was measured. Distilled water is used as a blank instead of a sample, a methanol solution is used as a sample blank instead of a DPPH methanol solution, and vitamin C is used as a positive control.
The clearance of the sample to DPPH radicals was calculated using the following formula:
DPPH radical scavenging = [1- (A1-A2)/A0 ] ×100%;
blank A0: absorbance of 2mL distilled water+2 mL DPPH solution;
sample A1: absorbance of 2mL sample solution+2 mL DPPH solution;
sample blank A2: absorbance of 2mL sample solution+2 mL methanol solution.
Table 2. Results of the test of DPPH radical scavenging by the compositions of examples 5-7 and comparative examples 1-4.
Sample of | DPPH radical scavenging Rate (%) |
Example 5 | 86.0 |
Example 6 | 83.6 |
Example 7 | 82.4 |
Comparative example 1 | 64.2 |
Comparative example 2 | 50.6 |
Comparative example 3 | 67.8 |
Comparative example 4 | 63.7 |
Positive control group | 40.3 |
As can be seen from Table 2, the antioxidant effect of the marine plant extract-containing composition prepared by compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract is better than that of the single component, so that the synergistic antioxidant effect of the marine plant extract-containing composition prepared by compounding the sargassum fusiforme extract, the centella asiatica extract, the artemisia capillaris extract and the black soybean extract is demonstrated.
The formula of the essence comprises the following components:
table 3. Formulation composition and dosage (kg) of essence.
Propylene glycol, glycerol, 1, 2-hexanediol, allantoin, ethylhexyl glycerol and water were mixed and stirred until dissolved, and the compositions prepared in examples 5 to 7 and comparative examples 1 to 4 were added respectively, and carbomer and xanthan gum were then added and stirred until dissolved, to obtain an essence.
Corrosion challenge experiment:
experimental strain: the bacterium was staphylococcus aureus ATCC6538 and the fungus was aspergillus niger ATCC16404, all from the collection of microorganisms of the cantonese province.
The test method comprises the following steps: referring to CTFA challenge experiment requirements, staphylococcus aureus 1.0X10 were inoculated in 5mL of essence prepared in examples 8-10 and comparative examples 5-8, respectively 6 CFU/g (CFU/mL) and Aspergillus niger 1.0X10 4 CFU/g (CFU/mL), samples after inoculation were isolated and tested at specific times.
And (3) judging a test result: results from 0 hours (i.e., sampling immediately after inoculation), 7 days, 14 days, 21 days and 28 days, required a 99.9% reduction in bacteria and 90% reduction in fungi at day 7; and continued to drop over 28 days, the corrosion challenge test was passed. The results are shown in Table 4.
Table 4. Antiseptic challenge test results for the essences prepared in examples 8-10 and comparative examples 5-8.
As can be seen from table 4, the essences prepared from the marine plant extract-containing compositions of examples 5 to 7 all passed the preservative challenge test, and had a strong preservative effect; the essences prepared from the single-component compositions of comparative examples 1-4 decreased in total bacteria and fungi over time, indicating some preservative efficacy, but failed the preservative challenge test.
Human skin patch test:
the purpose of the experiment is as follows: the safety of the essence containing the marine plant extract-containing composition prepared in examples 5 to 7 was examined.
The test method comprises the following steps: age composition and sex 100 volunteers were randomly selected, meeting 2007 standard of inclusion and exclusion of cosmetic hygiene; selecting skins at two sides of the upper back vertebra as tested parts, wherein the skins are divided into two parts, one part is blank control, and the other part is respectively smeared with the essence prepared in the examples 8-10; firmly attaching the spot tester adhesive tape with the spot test object from bottom to top; the patch test time is 48 hours; 48 hours after the application, the plaque tester was removed, and the test results were observed and recorded after 30 minutes.
Table 5. Criteria for grading adverse skin reactions.
Table 6. Skin patch test results.
As can be seen from table 6, the essence of the marine plant extract-containing composition was basically negative to the subjects, and was not seriously adverse effects, and as a result, it was determined that the essence of the marine plant extract-containing composition was not adverse effects to the human body.
Finally, it should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present invention after reading the present specification, and these modifications and variations do not depart from the scope of the invention as claimed in the pending claims.
Claims (3)
1. A composition comprising marine plant extract, characterized in that the composition comprises the following mass contents: 10-30% of sargassum fusiforme extract, 5-15% of centella asiatica extract, 5-15% of artemisia capillaris extract and 10-30% of black soybean extract;
the extraction process of the sargassum fusiforme extract comprises the following steps: reflux extracting dried and pulverized Cyrtymenia Sparsa with solvent, centrifuging, and concentrating supernatant to obtain Cyrtymenia Sparsa extract; the solvent is selected from 95% ethanol;
the extraction process of the centella asiatica extract comprises the following steps: mixing dried herba Centellae with deionized water, soaking, heating to boil, separating medicinal liquid, adding deionized water into the residue, heating to boil, mixing the medicinal liquids, filtering, centrifuging, and concentrating the supernatant to obtain herba Centellae extract;
the extraction process of the artemisia capillaris extract is as follows: mixing the dried and sieved herba Artemisiae Scopariae powder with the extractive solution, leaching, ultrasonic extracting, decolorizing, filtering, and concentrating to obtain herba Artemisiae Scopariae extract; the extract is selected from water and ethanol mixed solvent with the volume ratio of 1:9; the leaching temperature is 60-70 ℃;
the extraction process of the black soybean extract comprises the following steps: mixing the dried and sieved black soybean powder with the extract, leaching, regulating the pH value, performing ultrasonic extraction, filtering, decoloring, filtering and concentrating the filtrate to obtain a black soybean extract; the extract is selected from a mixed solvent of water and butanediol with the volume ratio of 2:8; the leaching temperature is 40-50 ℃; the pH value is 4.0-5.0.
2. Use of a marine plant extract-containing composition according to claim 1 for the preparation of a cosmetic product comprising the above marine plant extract-containing composition, said cosmetic product being selected from any one of a pack, an emulsion, a cream, a serum, a stock solution, a cleanser, a foundation, and a concealer.
3. The marine plant extract-containing composition according to claim 2, wherein the marine plant extract-containing composition is used in an amount of 0.1 to 0.5% by mass fraction of the cosmetic.
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