CN112111594A - InDel molecular marker for identifying multi-chamber character of brassica napus as well as primer group and application thereof - Google Patents
InDel molecular marker for identifying multi-chamber character of brassica napus as well as primer group and application thereof Download PDFInfo
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Abstract
The invention provides an InDel molecular marker for identifying multi-chamber characters of brassica napus, a primer group and application thereof, wherein the InDel molecular marker comprises N21 and N30. The molecular markers N21 and N30 are linked with the brassica napus multi-chamber gene Bnml1, and can be detected by common PCR and modified polyacrylamide gel electrophoresis. If the genome DNA of the leaves of the cabbage type rape to be detected is used as a template, a PCR amplification reaction is carried out by utilizing a primer pair for amplifying N21 and a primer pair for amplifying N30, and if the length of the N21 amplification product is 236bp and the length of the N30 amplification product is 144bp, the cabbage type rape to be detected is in an extreme multi-chamber character.
Description
Technical Field
The invention relates to the technical field of molecular assisted breeding, in particular to an InDel molecular marker for identifying multi-chamber characters of brassica napus, a primer group and application thereof.
Background
Rape is the first major oil crop in China. With the increasing demand of edible oil in China, the self-sufficient rate of edible oil in China is insufficient at present. Therefore, the development of the rape industry has great significance for guaranteeing the safety of edible oil in China, and the cultivation method of cultivating new varieties and reasonably matching by using technical means such as molecular assisted breeding and the like is an effective way for improving the yield of rape at present.
The factors of the single-plant yield of the rape include the effective pod number of the whole plant, the kernel number of each pod and the thousand kernel weight. Research shows that the yield of single plants is improved due to the increase of the number of grains per fruit of the multi-chamber rape. Therefore, the research of the multi-compartment character has important significance for improving the yield character of the rape.
The multi-chamber character in the cabbage type rape and the mustard type rape has a simple genetic rule, the multi-chamber character in the cabbage type rape is controlled by a pair of genes, the multi-chamber character in the mustard type rape is controlled by two pairs of genes, and the two-chamber character is completely dominant to the multi-chamber. The positioning of the multiple-chamber genes of cabbage type rape and mustard type rape is already completed at present, and the multiple-chamber genes are cloned by a map-based cloning method. However, the location of multi-compartment genes and molecular markers linked with the genes in the brassica napus are not reported.
Disclosure of Invention
The invention aims to provide an InDel molecular marker for identifying the multi-chamber character of brassica napus as well as a primer group and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an InDel molecular marker for identifying multi-chamber characters of brassica napus, which comprises N21 and N30; the N21 is positioned at 18005958-18006193 bp of ChrA 03; the N30 is positioned at 22842092-22842239 bp of ChrA 03; the sites of N21 and N30 are the Brassica napus Darmor v4.1 as reference genome.
The invention also provides a primer group for amplifying the InDel molecular marker in the scheme, which comprises a primer pair N21-F and N21-R for amplifying N21 and a primer pair N30-F and N30-R for amplifying N30; the nucleotide sequence of N21-F is shown as SEQ ID NO.1, and the nucleotide sequence of N21-R is shown as SEQ ID NO. 2; the nucleotide sequence of N30-F is shown as SEQ ID NO.3, and the nucleotide sequence of N30-R is shown as SEQ ID NO. 4.
The invention also provides application of the InDel molecular marker or the primer group in the scheme in rape molecular assisted breeding.
The invention also provides application of the InDel molecular marker or the primer group in the scheme in identifying the multi-chamber character of the brassica napus.
Preferably, the brassica napus comprises brassica napus 1332.
Preferably, the application comprises the following steps:
1) extracting genome DNA of leaves of the cabbage type rape to be detected;
2) respectively carrying out PCR amplification reaction by using the genome DNA of the brassica napus leaves to be detected as a template and using a primer pair for amplifying N21 and a primer pair for amplifying N30 to respectively obtain an N21 amplification product and an N30 amplification product;
3) detecting the N21 amplification product and the N30 amplification product respectively by using denaturing polyacrylamide gel electrophoresis; if the length of the N21 amplification product is 236bp and the length of the N30 amplification product is 144bp, the cabbage type rape to be detected is in an extreme multi-chamber property.
Preferably, the cabbage type rape leaves in the step 1) are cabbage type rape leaves in a seedling stage.
Preferably, the PCR amplification reaction of the primer pair for amplifying N21 is 10 μ L, and comprises the following components: template 1. mu. L, dNTPs 0.8.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu. L, N21-F0.5. mu. L, N21-R0.5. mu.L and ddH2O 6.1μL；
The PCR amplification reaction of the primer pair for amplifying the N30 comprises the following components in 10 mu L: template 1. mu. L, dNTPs 0.8.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu. L, N30-F0.5. mu. L, N30-R0.5. mu.L and ddH2O 6.1μL。
Preferably, the reaction procedure of the PCR amplification reaction in step 2) is: 94 ℃ for 10 min; 94 ℃, 10s, 55 ℃, 30s, 72 ℃, 45s, 34 cycles; 72 ℃ for 10 min; at 4 deg.C for 30 min.
The invention has the beneficial effects that: the invention provides an InDel molecular marker for identifying multi-chamber characters of brassica napus, which comprises N21 and N30. The molecular markers N21 and N30 are linked with the multi-chamber gene Bnml1 of the Brassica napus, and can be detected by common PCR and polyacrylamide gel electrophoresis. If the genome DNA of the leaves of the cabbage type rape to be detected is used as a template, a PCR amplification reaction is carried out by utilizing a primer pair for amplifying N21 and a primer pair for amplifying N30, and if fragments with the lengths of 236bp and 144bp are simultaneously amplified, the cabbage type rape to be detected is in an extreme multi-chamber character.
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FIG. 1 shows the molecular marker N21 at BC1(iii) amplification results in populations 1345-1376;
FIG. 2 shows the molecular marker N30 at BC1Amplification results in populations 1345-1376.
Detailed Description
The invention provides an InDel molecular marker for identifying multi-chamber characters of brassica napus, wherein N21 is positioned at 18005958-18006193 bp of ChrA03, 6bp of a double-chamber parent is deleted at a position of 18006041-18006046 bp compared with a multi-chamber parent, and the deleted specific sequence is GCTGCA; the N30 is located at 22842092-22842239 bp of ChrA03, the multi-chamber parent is deficient in 4bp at 22842205-22842208 bp compared with the two-chamber parent, and the specific sequence is CTTT. In the invention, the sites of N21 and N30 are the Brassica napus Darmor v4.1 serving as a reference genome.
In the invention, the molecular markers N21 and N30 are linked with the multi-chamber gene Bnml1 of the Brassica napus, and can be detected by common PCR and polyacrylamide gel electrophoresis. The amplified fragment of N21 in the brassica napus is 236bp, and the amplified fragment of N30 in the brassica napus is 144 bp. If the genome DNA of the leaves of the cabbage type rape to be detected is used as a template, a PCR amplification reaction is carried out by utilizing a primer pair for amplifying N21 and a primer pair for amplifying N30, and if fragments with the lengths of 236bp and 144bp are simultaneously amplified, the cabbage type rape to be detected is in an extreme multi-chamber character.
The invention also provides a primer group for amplifying the InDel molecular marker in the scheme, which comprises a primer pair N21-F and N21-R for amplifying N21 and a primer pair N30-F and N30-R for amplifying N30; the nucleotide sequence of N21-F is shown as SEQ ID NO.1, and specifically comprises the following steps: aacaaccaggttctgcagca, respectively; the nucleotide sequence of N21-R is shown as SEQ ID NO.2, and specifically comprises the following steps: tcactcacccgattgttgct, respectively; the nucleotide sequence of N30-F is shown as SEQ ID NO.3, and specifically comprises the following steps: ttggtttcgcccctgttgat, respectively; the nucleotide sequence of N30-R is shown as SEQ ID NO.4, and specifically comprises the following steps: gggtggatggcagtttcttt are provided.
The invention also provides application of the InDel molecular marker or the primer group in the scheme in rape molecular assisted breeding.
The invention also provides application of the InDel molecular marker or the primer group in the scheme in identifying the multi-chamber character of the brassica napus.
In the present invention, the application preferably comprises the steps of:
1) extracting genome DNA of cabbage type rape leaves to be detected in a seedling stage;
2) and respectively carrying out PCR amplification reaction by using the genome DNA of the brassica napus leaves to be detected as a template and using a primer pair for amplifying N21 and a primer pair for amplifying N30 to respectively obtain an N21 amplification product and an N30 amplification product:
3) detecting the N21 amplification product and the N30 amplification product respectively by using denaturing polyacrylamide gel electrophoresis; if the length of the N21 amplification product is 236bp and the length of the N30 amplification product is 144bp, the cabbage type rape to be detected is in an extreme multi-chamber property.
The method for extracting the genome DNA of the brassica napus leaves to be detected is not particularly limited, and the method for extracting the genome DNA of the conventional plants in the field can be adopted.
After the genome DNA of the cabbage type rape leaves to be detected is obtained, the genome DNA of the cabbage type rape leaves to be detected is taken as a template, and PCR amplification reactions are respectively carried out by utilizing a primer pair for amplifying N21 and a primer pair for amplifying N30 to respectively obtain an N21 amplification product and an N30 amplification product; the PCR amplification reaction using the primer pair for amplifying N21 is preferably composed of the following components in 10. mu.L: template 1. mu. L, dNTPs 0.8.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu. L, N21-F0.5. mu. L, N21-R0.5. mu.L and ddH2O6.1 μ L; the PCR amplification reaction using the primer pair for amplifying N30 is preferably composed of the following components in 10. mu.L: template 1. mu. L, dNTPs 0.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu.L,N30-F0.5. mu. L, N30-R0.5. mu.L and ddH2O 6.1μL。
After PCR amplification products are obtained, detecting the N21 amplification product and the N30 amplification product respectively by utilizing denaturing polyacrylamide gel electrophoresis; if the length of the N21 amplification product is 236bp and the length of the N30 amplification product is 144bp, the cabbage type rape to be detected is in an extreme multi-chamber property.
In the invention, the nucleotide sequence of the amplified sequence of N21 in the multi-chamber rape is shown as SEQ ID NO.5, and is specifically (5 '-3'):
the nucleotide sequence of the amplified sequence of N30 in the multi-chamber rape is shown as SEQ ID NO.6, and is specifically (5 '-3'):
in the present invention, the nucleotide sequence of the amplified sequence of N21 in the two-chamber parent is shown in SEQ ID NO.7, specifically (5 '-3'):
the nucleotide sequence of the amplified sequence of N30 in the two-chamber parent is shown as SEQ ID NO.8, and is specifically (5 '-3'):
the subsidized items of the invention include:
1. breeding seed topic of new transgenic rape in spring rape area (2018ZX 0802-0001-one 009);
2. qinghai province "high-end innovation talent thousands people plan".
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Hybridizing by using cabbage type two-chamber rape green oil No. 14 as female parent and cabbage type multi-chamber rape 1332 as male parent to obtain F1Then, the cabbage type multi-chamber rape is used as a recurrent parent to obtain BC1And (4) a group. The brassica napus multi-chamber rape has the same characteristics as the common brassica napus (the rape with the silique character of two chambers) except the multi-chamber silique characteristic.
(2) Investigation BC1Multi-compartment silique rate of individual plants of the population. And counting the total silique number and the multi-chamber silique number of each single plant in the green maturity stage, and calculating the multi-chamber silique rate of the single plant. Sorting the multi-chamber silique rate of the single plant from small to large, and selecting 23 single plants with the highest and the lowest multi-chamber silique rate respectively.
Multi-compartment silique rate (multi-compartment silique/total silique) 100%
(3) BSA analysis
(a) DNA extraction: a CTAB method is used for extracting 46 strains of parent green oil No. 14, cabbage type multi-chamber rape 1332 and the selected single plant with the highest and the lowest multi-chamber silique rate, and one strain of the two-chamber parent and one strain of the multi-chamber parent are used for obtaining 48 strains in total.
(b) The 23 single plant DNAs with the lowest multi-chamber silique rate were mixed in equal amounts to construct a two-chamber mixed pool, and the 23 single plant DNAs with the highest multi-chamber silique rate were mixed in equal amounts to construct a multi-chamber mixed pool.
(c) Library construction and sequencing: and performing quality inspection on the parent sample for sequencing and the isolated population extreme single plant mixed pool, constructing a PE library by using the qualified sample for detection, and constructing a PE library by using each sample DNA. Randomly breaking the genome DNA into fragments of 300-500 bp, and connecting with a sequencing joint. After preparation of the DNA clusters (DNA cluster) on the sequencing chip, PE150 sequencing was performed on an Illumina HiSeq sequencer.
(d) And (3) mutation detection: cutadapt and Trimmomatic software are used for carrying out quality control on the original data of the machine unloading, the obtained high-quality clean data is compared with a reference genome (Darmor v4.1), and mutation detection is carried out. The BAM file which can be finally used for variable sealing is obtained through BWA software, Samtools software and SortSam processing in Picard tool. Finally, a HaplotpypeCaller module of the GATK is used for carrying out the variation detection of the SNP and the InDel, and the distribution of the obtained SNP and the InDel variation on each chromosome is shown in Table 1.
TABLE 1 distribution of available SNPs and InDel on individual chromosomes
Note: chr is chromosome number, Length is chromosome Length, SNPs is number and density of SNPs, and InDels is number and density of InDel.
(e) And determining candidate intervals.
Based on the SNP/InDel markers obtained after screening, in order to exclude the influence of unreliable markers, SNP-index was first screened using the following conditions: the sequencing depth of both parents is more than 8, the sequencing depth of both pools is more than 10, the SNP-index values of both pools cannot be simultaneously more than 0.8 or simultaneously less than 0.2, and the polymorphism exists between the parents (the difference of the SNP-index values is more than 0.8). Based on the selected markers, Δ (SNP-index) and G' were calculated using QTLseqr software, respectively, and the sliding window size was set to 2 Mb. Thresholds for Δ (SNP-index) and G' were determined at 98.5% and 99.99% confidence levels, respectively, and regions above the threshold were selected as regions associated with traits, and regions overlapping under both algorithms were determined as final candidate intervals.
(4) Development of InDel markers
And selecting InDel primers in a target interval according to a re-sequencing result, and detecting polymorphism in parent and extreme gene pools to obtain 15 pairs of InDel markers with polymorphism.
(5) Construction of local genetic linkage map
Using the screened InDel polymorphic primer pair BC1Extreme phenotype in the populationAnd detecting the individual plants, and counting the result of each mark. A genetic linkage map is constructed by using the JeonMap 4.0, the recombination rate is converted into a genetic distance by a Kosambi function, the genetic map is drawn, and finally the Bnml1 is limited between the N21 and the N30.
Example 2
1) BC in a different line from the above1And after the multi-chamber silique rate is investigated, the multi-chamber silique rate is sequenced from small to large, and then 20 single plants in two chambers and 20 single plants in each chamber are selected.
2) The DNA of these 40 individuals was extracted according to the CTAB method.
3) Detection was performed by polyacrylamide gel electrophoresis after PCR amplification using N21 and N30 markers.
4) Extreme two-compartment and multi-compartment individuals can be distinguished by combining N21 and N30 markers, wherein A represents a N21 marker two-compartment parent genotype, B represents a N21 marker multi-compartment parent genotype, H represents a N21 marker heterozygous type, B represents a N21 marker multi-compartment homozygous genotype, C represents a N30 marker two-compartment parent genotype, D represents a N30 marker multi-compartment parent genotype, E represents a N30 marker heterozygous type, and D represents a N30 marker multi-compartment homozygous genotype. The results of molecular markers show that the amplification results of the N21 and N30 markers with the multi-chamber silique rate range of 0-8.42 percent are both heterozygous genotypes, and the amplification results of the single plant N21 and N30 markers with the multi-chamber silique rate range of 77.25-98.41 percent are both recessive homozygous genotypes consistent with the multi-chamber parents, so that the N21 and N30 markers can distinguish BC1Extreme two-compartment and multi-compartment behavior in the population. The result of combining two molecular markers can distinguish between extreme two-compartment and multi-compartment individuals. If the fragments with the lengths of 236bp and 144bp are amplified simultaneously, the cabbage type rape to be detected is of extreme multi-chamber character; if the fragments with the lengths of 230bp and 148bp simultaneously appear, the cabbage type rape to be detected is the extreme two-chamber character.
See table 2, fig. 1 and fig. 2 for results.
TABLE 2 BC1Extreme Individual Multi-Chamber silique Rate in the population and results of genotyping of the N21 and N30 markers
As can be seen from Table 2, the N21 marker amplified the bands of 230bp and 236bp in the bipartite parent and the multicompartment parent, respectively, at BC1The amplified bands in the extreme two-chamber material of the population comprise two bands of 230bp and 236bp, and only a 236bp band is amplified in the extreme multiple-chamber material. The N30 marker amplified the length of the bands in the bipartite parent and the multicompartment parent, 148bp and 144bp respectively, at BC1The amplified bands in the extreme two-chamber material of the population comprise two bands of 148bp and 144bp, and only a band of 144bp is amplified in the extreme multiple-chamber material.
In FIGS. 1 and 2, the 1 and 2 wells are amplification band patterns of the two-chamber parent green oil No. 14, the 3 and 4 wells are amplification band patterns of the multi-chamber parent 1332, the 5 to 24 wells are amplification band patterns of a single plant with a phenotype expressed as an extreme two-chamber, the multi-chamber silique rate thereof is shown in Table 2, the 25 to 44 wells are amplification band patterns of a single plant with a phenotype expressed as an extreme multi-chamber, and the multi-chamber silique rate thereof is shown in Table 2. The amplification results of FIGS. 1 and 2 show that N21 and N30, as co-dominant markers, can distinguish between dominant homozygous, heterozygous and recessive homozygous genotypes. The scanning results of the N21 and N30 on the extreme double-chamber single plants are both heterozygous genotypes, and the scanning results of the extreme double-chamber single plants are recessive homozygous, so that the results of the N21 and N30 molecular marker scanning are combined with BC1The extreme two-compartment and multi-compartment phenotypes in the population have good correspondence.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> academy of agriculture and forestry of Qinghai province
<120> InDel molecular marker for identifying brassica napus multi-chamber character, primer group and application thereof
<160> 8
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
aacaaccagg ttctgcagca 20
<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
tcactcaccc gattgttgct 20
<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
ttggtttcgc ccctgttgat 20
<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
gggtggatgg cagtttcttt 20
<210> 5
<211> 236
<212> DNA
<213> Brassica napus (Brassica napus L.)
<400> 5
aacaaccagg ttctgcagca gcgtgtcctc cagttagcac cacgggatcc gttatggcca 60
cttcagatcc agcgaaagcc atggctgcag cagcagcagg caataactta aaaggaggag 120
ggaagacgca gcaacatcag ctgggacctc ctgggttcgg gctcccgtat gttcacgcgg 180
ttccctgtgc agttcaagtt aaacctgtag atcagaagca acaatcgggt gagtga 236
<210> 6
<211> 144
<212> DNA
<213> Brassica napus (Brassica napus L.)
<400> 6
ttggtttcgc ccctgttgat aacagtacga aatatcgcta aagtcttacc cttttgtgat 60
tgctaatcaa attaattaaa atgaacaaaa aaggaattca aaccaaagta aacttttggt 120
cgaaaaagaa actgccatcc accc 144
<210> 7
<211> 230
<212> DNA
<213> Brassica napus (Brassica napus L.)
<400> 7
aacaaccagg ttctgcagca gcgtgtcctc cagttagcac cacgggatcc gttatggcca 60
cttcagatcc agcgaaagcc atggcagcag caggcaataa cttaaaagga ggagggaaga 120
cgcagcaaca tcagctggga cctcctgggt tcgggctccc gtatgttcac gcggttccct 180
gtgcagttca agttaaacct gtagatcaga agcaacaatc gggtgagtga 230
<210> 8
<211> 148
<212> DNA
<213> Brassica napus (Brassica napus L.)
<400> 8
ttggtttcgc ccctgttgat aacagtacaa aatatcgcta aagtcttacc cttttgtgat 60
tgctaatcat attaattaaa atgaacaaaa aaggaactca aaccaaagta aacctttttt 120
tggtcgaaaa agaaactgcc atccaccc 148
Claims (9)
1. An InDel molecular marker for identifying the multi-compartment character of Brassica napus, wherein the InDel molecular marker comprises N21 and N30; the N21 is positioned at 18005958-18006193 bp of ChrA 03; the N30 is positioned at 22842092-22842239 bp of ChrA 03; the sites of N21 and N30 are the Brassica napus Darmor v4.1 as reference genome.
2. A primer set for amplifying the InDel molecular marker of claim 1, comprising a primer pair N21-F and N21-R for amplifying N21 and a primer pair N30-F and N30-R for amplifying N30; the nucleotide sequence of N21-F is shown as SEQ ID NO.1, and the nucleotide sequence of N21-R is shown as SEQ ID NO. 2; the nucleotide sequence of N30-F is shown as SEQ ID NO.3, and the nucleotide sequence of N30-R is shown as SEQ ID NO. 4.
3. The use of the InDel molecular marker of claim 1 or the primer set of claim 2 in molecular assisted breeding of Brassica napus.
4. The use of the InDel molecular marker of claim 1 or the primer set of claim 2 for identifying multi-compartment traits of Brassica napus.
5. The use according to claim 3 or 4, wherein the Brassica napus comprises Brassica napus 1332.
6. The application according to claim 3 or 4, characterized in that it comprises the following steps:
1) extracting genome DNA of leaves of the cabbage type rape to be detected;
2) respectively carrying out PCR amplification reaction by using the genome DNA of the brassica napus leaves to be detected as a template and using a primer pair for amplifying N21 and a primer pair for amplifying N30 to respectively obtain an N21 amplification product and an N30 amplification product;
3) detecting the N21 amplification product and the N30 amplification product respectively by using denaturing polyacrylamide gel electrophoresis; if the length of the N21 amplification product is 236bp and the length of the N30 amplification product is 144bp, the cabbage type rape to be detected is in an extreme multi-chamber property.
7. The use according to claim 6, wherein the brassica napus leaves in step 1) are brassica napus leaves at seedling stage.
8. The use according to claim 6, wherein the PCR amplification reaction of the primer pair for amplifying N21 in step 2) is 10 μ L and comprises the following components: template 1. mu.L, dNTPs0.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu. L, N21-F0.5. mu. L, N21-R0.5. mu.L and ddH2O 6.1μL；
The PCR amplification reaction of the primer pair for amplifying the N30 comprises the following components in 10 mu L: template 1. mu. L, dNTPs 0.8.8. mu. L, taq enzyme 0.1. mu.L, 10 XBuffer 1. mu. L, N30-F0.5. mu. L, N30-R0.5. mu.L and ddH2O 6.1μL。
9. The use according to claim 7, wherein the reaction procedure of the PCR amplification reaction in step 2) is: 94 ℃ for 10 min; 94 ℃, 10s, 55 ℃, 30s, 72 ℃, 45s, 34 cycles; 72 ℃ for 10 min; at 4 deg.C for 30 min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528698A (en) * | 2021-07-14 | 2021-10-22 | 中国农业科学院油料作物研究所 | InDel molecular marker for identifying and/or distinguishing cabbage vegetables and application thereof |
| CN115948599A (en) * | 2022-12-29 | 2023-04-11 | 中国农业科学院油料作物研究所 | InDel molecular marker of RAD17 gene and detection primer and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528698A (en) * | 2021-07-14 | 2021-10-22 | 中国农业科学院油料作物研究所 | InDel molecular marker for identifying and/or distinguishing cabbage vegetables and application thereof |
| CN113528698B (en) * | 2021-07-14 | 2022-01-25 | 中国农业科学院油料作物研究所 | InDel molecular marker for identifying and/or distinguishing cabbage vegetables and application thereof |
| CN115948599A (en) * | 2022-12-29 | 2023-04-11 | 中国农业科学院油料作物研究所 | InDel molecular marker of RAD17 gene and detection primer and application thereof |
| CN115948599B (en) * | 2022-12-29 | 2023-09-08 | 中国农业科学院油料作物研究所 | InDel molecular marker of RAD17 gene, detection primer and application thereof |
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