CN113736910B - Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof - Google Patents

Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof Download PDF

Info

Publication number
CN113736910B
CN113736910B CN202111185756.4A CN202111185756A CN113736910B CN 113736910 B CN113736910 B CN 113736910B CN 202111185756 A CN202111185756 A CN 202111185756A CN 113736910 B CN113736910 B CN 113736910B
Authority
CN
China
Prior art keywords
peanut
molecular
seq
pod number
molecular marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202111185756.4A
Other languages
Chinese (zh)
Other versions
CN113736910A (en
Inventor
张胜忠
陈静
胡晓辉
苗华荣
王菲菲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Peanut Research Institute
Original Assignee
Shandong Peanut Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Peanut Research Institute filed Critical Shandong Peanut Research Institute
Priority to CN202111185756.4A priority Critical patent/CN113736910B/en
Publication of CN113736910A publication Critical patent/CN113736910A/en
Application granted granted Critical
Publication of CN113736910B publication Critical patent/CN113736910B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a main effect QTL locus of peanut single plant pod numberqPN7Is a linkage molecular marker and application thereof. The linkage molecular Marker is positioned on the 7 th chromosome of the peanut genome and comprises a molecular Marker4240, a molecular Marker4243 and a molecular Marker4251. The nucleotide sequence of the molecular Marker4240 is shown in SEQ ID No. 1; the nucleotide sequence of Marker4243 is shown in SEQ ID No. 3; the nucleotide sequence of Marker4251 is shown as SEQ ID No. 5. The linkage molecular marker is obtained by using a primer composition. The invention discloses a main effect QTL locus of peanut single plant pod numberqPN7And the linkage molecular marker and the identification method are suitable for peanut individual pod number gene positioning and molecular marker assisted selective breeding.

Description

Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof
Technical Field
The invention belongs to the technical field of peanut molecular genetic breeding, and in particular relates to a main effect QTL locus of peanut single plant pod numberqPN7Is a linkage molecular marker and application thereof.
Background
PeanutArachis hypogeaL.) is one of five oil crops worldwide, and is also an important source of edible oil and protein in our country. In recent years, along with the growth of the economy and population of China, the peanut consumption demand is in a rigid growth situation, and the self-supporting capability of peanuts in China faces a great challenge. The method for improving the single yield of the peanuts by variety improvement is a main way for improving the total yield of the peanuts and meeting the balance of supply and demand of the market on the premise of cultivated land resource constraint.
The pod number of a single plant is one of peanut yield traits, belongs to quantitative traits and is easy to influence by environment. In traditional breeding, the effect of offspring selection by only phenotype is poor, and the breeding efficiency is low. By constructing a high-density genetic linkage map, positioning and controlling the gene/QTL locus of the trait and developing a linkage molecular marker, and utilizing the molecular marker to carry out auxiliary selection, the prediction, verification and polymerization of the target trait can be effectively realized, and the breeding efficiency is remarkably improved.
In recent years, researchers at home and abroad perform genetic research on various peanut yield traits, and a batch of QTLs for controlling the peanut pod size, pod weight, kernel size, kernel weight and other traits are detected. However, currently, there are few positioning researches on peanut single-plant pod numbers, and reports on related major QTL and linkage molecular markers are absent. Therefore, screening a peanut single-plant pod number main effect QTL locus capable of being stably expressed has important significance for accelerating cloning of peanut single-plant pod number gene map locus and cultivating new peanut varieties with excellent single-plant pod number characters.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a main effect QTL locus for the pod number of a peanut single plantqPN7Is a linkage molecular marker and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
the invention provides a main effect QTL locus for the pod number of a peanut single plantqPN7The linked molecular marker of (2) is a major QTL locus of peanut pod numberqPN7Is co-located on the 7 th chromosome of peanut genome and comprises a molecular Marker4240, a molecular Marker4243 and a molecular Marker4251.
Further, the nucleotide sequence of the molecular Marker4240 is shown as SEQ ID No. 1; the nucleotide sequence of Marker4243 is shown in SEQ ID No. 3; the nucleotide sequence of Marker4251 is shown as SEQ ID No. 5.
Furthermore, the polymorphism of the molecular Marker4240 is A/G, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.2; the polymorphism of the molecular Marker4243 is T/C, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.4; the polymorphism of the molecular Marker4251 is C/T, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.6.
The invention also provides a primer composition, which comprises 6 primers, and the nucleotide sequences of the primers are respectively shown as SEQ ID No. 7-SEQ ID No. 12.
Further, the primer composition contains a primer of an amplified molecular Marker4240 with nucleotide sequences shown as SEQ ID No.7 and SEQ ID No.8, a primer of an amplified molecular Marker4243 with nucleotide sequences shown as SEQ ID No.9 and SEQ ID No.10, and a primer of an amplified molecular Marker4251 with nucleotide sequences shown as SEQ ID No.11 and SEQ ID No. 12.
The invention also provides a main effect QTL locus of the pod number of the peanut single plantqPN7The identification method comprises the following steps:
extracting genome DNA of peanut materials to be identified, carrying out PCR amplification on the template by using the primer composition and carrying out sequencing analysis on the amplified product by taking the genome DNA as a template, and judging polymorphism of the linkage molecular marker according to a sequencing result.
Further, according to the sequencing result of the peanut material to be identified, if the linked molecular marker has polymorphism, that is, the nucleotide sequence of the linked molecular marker is the same as that of the strain 6-13, judging that the number of single plant pods of the peanut material is more than or equal to 20; if the linked molecular marker does not have polymorphism, namely the nucleotide sequence of the linked molecular marker is the same as that of the variety Huayu No. 36, judging that the number of single plant pods of the peanut material is less than 20.
Furthermore, the base of the line 6-13 at the 801 st position of the molecular Marker4240 is G, and the base of the line flower and breed 36 at the 801 st position of the molecular Marker4240 is A; the base at the 801 st position of a molecular Marker4243 of the strain 6-13 is C, and the base at the 801 st position of the molecular Marker4243 of the strain flower and plant 36 is T; the base at the 801 st position of the molecular Marker4251 of the strain 6-13 is T, and the base at the 801 st position of the molecular Marker4251 of the strain flower and plant 36 is C.
Further, the PCR amplification reaction program is as follows: 94 ℃ for 5 min;98 ℃ for 10 s,60 ℃ for 30 s and 68 ℃ for 1 min, and 35 cycles are total; extension at 68deg.C for 10 min, and preservation at 4deg.C.
Further, the reaction system of the PCR amplification is 50 mu L,specifically, the kit comprises 2 mu L of 10-20 ng DNA template, 1.5 mu L of primer pairs, 10 mu L of dNTP, 25 mu L of KOD buffer solution, 1 mu L of KOD polymerase and ddH 2 O 9 μL。
The invention also provides a main effect QTL locus of peanut single plant pod numberqPN7Application of peanut single plant pod number trait in regulation and control, wherein peanut single plant pod number major QTL (quantitative trait locus)qPN7The physical position of the genome is between the 7 th chromosome 208645 and 1146586 base positions of the peanut genome, and the genome can be detected by a primer shown in SEQ ID No. 7-SEQ ID No. 12; main effect QTL locus of peanut single plant pod numberqPN7From peanut lines 6-13, is capable of increasing the pod number of a peanut individual.
The invention also provides an application of the linkage molecular marker or the primer composition in breeding high single-plant pod number peanut varieties or strains.
The invention also provides application of the linkage molecular marker or the primer composition in peanut molecular breeding, transgenic peanut cultivation or peanut germplasm resource improvement.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention discloses a major QTL locus of peanut single plant pod number of synergistic alleles from cultivated peanut lines 6-13 for the first timeqPN7The peanut strain is positioned between 7 th chromosome 208645-1146586 base positions of a genome of the cultivated peanut, different genotypes have different regulation and control effects on the number of single peanut plants, and the peanut strain has higher utilization value for breeding peanut varieties or strains with high single peanut plant numbers.
2. The invention firstly screens and discloses the main effect QTL locus of the pod number of the peanut single plantqPN7The three linked molecular markers Marker4240, marker4243 and Marker4251, and the molecular Marker primer pair and the molecular Marker method for amplifying the linked molecular markers can be effectively applied to map-position cloning of peanut individual pod genes, assist in selection of peanut individual pod number character offspring, facilitate improvement of breeding efficiency and accelerate genetic improvement of peanut individual pod number characters.
Drawings
FIG. 1 shows a peanut sheet according to the present inventionMajor loci of pod number of plantqPN7Positioning at the 7 th chromosome, wherein a vertical column represents the 7 th chromosome of peanut, and a short horizontal line on the vertical column represents a molecular marker; with the number of single peanut pod active sitesqPN7The 3 linked molecular markers are Marker4251, marker4240 and Marker4243; the right rectangle of the vertical column represents the main effect QTL locus detected under 4 different environmentsqPN7Is a confidence interval of (2).
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention may be made by those skilled in the art without departing from the spirit and scope of this invention.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
Test material: hybridization is carried out by taking peanut variety flower breeding No. 36 as female parent and peanut strain 6-13 as male parent, and Recombinant Inbred Line (RIL) population F containing 181 families is obtained by single grain transmission method 9 (2019) and F 10 In the (2020) generation, the number of single-plant pods for female parent flower cultivation No. 36 is 15-20, and the number of single-plant pods for male parent 6-13 is 24-28, both of which can be purchased from peanut research in Shandong province.
1. Phenotypic identification: the materials to be tested are respectively planted in the eastern city (37.31 degrees N,118.62 degrees E) and the Qingdao city (36.82 degrees N,120.51 degrees E) test bases in 2019; plant in 2020 on tobacco stand (37.18°n,121.51 °e) and wegian (37.18°n,121.99 °e) test bases; the planting mode is ridging single-row single-grain film-covered planting, the ridge distance is 0.3 m, 10 plants are planted in each row, the plant distance is 0.2 m, the random block design is adopted, and 3 times of repetition are arranged. The test base has uniform fertility, conventional field management and timely disease and pest control. The middle 8 individual mature pods were harvested in each row and the number of mature pods per plant was counted.
The results show that the individual pod number variation ranges of RIL groups under 4 different environments (2019 east ying, 2019 Qingdao, 2020 smoke table, 2020 Weihai) are 6.08-58.16, 5.00-57.40, 7.11-42.10 and 5.74-41.51 respectively, and the results show super-parent genetics (Table 1), so that the groups are suitable for carrying out peanut individual pod number trait QTL analysis.
TABLE 1 RIL population individual pod number phenotype data under 4 environments
Figure 446530DEST_PATH_IMAGE002
2. Construction of a genetic map: genomic DNA of variety flower-growing No. 36, strain 6-13 and 181 RIL families were extracted using the root DNA extraction kit.
SNP typing: sequencing library construction is carried out according to SLAF library construction flow, and after the library quality is checked to be qualified, illumina HiSeq is used TM The 2500 platform was sequenced, the parental sequencing depth was 51 Xor more, the RIL family sequencing depth was 16 Xor more, the raw data after the machine was taken off were subjected to sequencing quality assessment, and the high quality reads were aligned to the peanut reference genome (https:// www.peanutbase.org/data/v 2/Arachis/hypogaea/genome/Tifrunner.gnm1. KYV3 /). SNP detection is mainly achieved using the GATK software toolkit. The SLAF tag is filtered according to the following rules: (1) filtering tags sequenced to a depth of less than 10 x in the parent; (2) filtering the biased separated tags; (3) filtering tags with an integrity of less than 70%. Aa Xbb type polymorphism SLAF tags were aligned to peanut reference genome (https:// www.peanutbase.org/data/v 2/Arachis/hypogaea/genome/Tifrenner. Gnm1.KYV3 /) by BWA software according to RIL population type. High-quality SNP is obtained after filtration and is used for subsequent genetic map construction.
SSR typing: SSR markers with polymorphisms in variety Huayu No. 36 and lines 6-13 are screened by using published SSR markers (https:// www.peanutbase.org/search/marker), and then SSR typing is carried out on RIL families. The PCR reaction system is as follows: 1. Mu.L of DNA template, 2. Mu.L of primer pair (0.2. Mu.M) in total (1. Mu.L each), 2X Taq PCR Master Mix. Mu.L, ddH 2 O2. Mu.L. The PCR reaction procedure involved was: 94 ℃ for 3 min;98 ℃ for 1 min,5530 s at a temperature of 72 ℃ and 90 s for 35 cycles; extension at 72deg.C for 10 min is terminated, and stored at 4deg.C. And (3) carrying out electrophoresis analysis on the PCR amplification product by using a 6% PAGE gel to obtain RIL family SSR typing data.
Genetic map construction: high quality SNP and SSR markers are assigned to different linkage groups based on paired MLOD values, and the HighMap strategy is used to rank SNP and SSR markers and correct false marking patterns. Obtaining the marking sequence of each linkage group by using MSTmap, correcting the error marking sequence by using SMOOTH algorithm, predicting the deletion marking genotype by using k-nearest neighbor algorithm, and estimating the genetic distance of the linkage group based on Kosambi mapping function.
Genetic map information: contains 3866 marker loci distributed in 20 linkage groups, and has a coverage genetic map distance of 1,266.87 cM and an average distance between markers of 0.33 cM.
3. QTL positioning analysis: by using the constructed genetic map and phenotype data of 4 environments, a main effect QTL locus is detected on the 7 th chromosome of a reference genome of the peanut cultivar by adopting a complete interval mapping method of QTL Icimapping V4.1 softwareqPN7As shown in figure 1, the QTL can be stably expressed in 4 environments, the LOD variation range is 4.50-8.19, the contribution rate variation range is 12.76-16.92%, the synergistic allele is from strain 6-13, and the physical position is between the 7 th chromosome 208645-1146586 base position of the genome of the cultivated species peanut.
To further densify the map, a major QTL locus was obtainedqPN7Closely linked molecular markers: molecular Marker4240, molecular Marker4243 and molecular Marker4251.
The physical position of the molecular Marker4240 is Arahy.07:208645 bp (A/G) of peanut reference genome, the sequence of the upstream 800: 800 bp is shown as SEQ ID No.1, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.2; the physical position of the molecular Marker4243 is Arahy.07:811108 bp (T/C) of peanut reference genome, the sequence of the upstream 800: 800 bp is shown as SEQ ID No.3, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.4; the physical position of the molecular Marker4251 is Arahy.07:1146586 bp (C/T) of peanut reference genome, the sequence of the upstream 800: 800 bp is shown as SEQ ID No.5, and the nucleotide sequence with polymorphism difference is shown as SEQ ID No.6.
Example 2
Utilizing the main effective site of peanut pod numberqPN7And carrying out PCR amplification on the genome DNA of the RIL population, the variety Huayu 36 and the strain 6-13 by using 3 linked molecular marker primer pairs, and further judging the polymorphism of the RIL family in 3 molecular marker sequences.
Major QTL locus for amplifying peanut single plant pod numberqPN7Primer pair of linked molecular Marker 4240:
forward primer sequence 5'-ATTTCAAGGGGACACTCTGC-3' (shown as SEQ ID No. 7);
reverse primer sequence 5'-TGTCAAGTGGCCCGGTTTA-3' (shown as SEQ ID NO. 8).
The target base of the variety flower line 36 in the molecular Marker4240 is A (the 801 st base shown in SEQ ID No. 1), and the target base of the variety 6-13 in the molecular Marker4240 is G (the 801 st base shown in SEQ ID No. 2).
Major QTL locus for amplifying peanut single plant pod numberqPN7Primer pair of linked molecular Marker 4243:
forward primer sequence 5'-ATAACAATCACCCTGATTTCG-3' (shown as SEQ ID No. 9);
reverse primer sequence 5'-CAACTCCAACTGCTGTCGTC-3' (shown as SEQ ID NO. 10).
The target base of the molecular Marker4243 of the variety flower is T (the 801 st base shown in SEQ ID No. 3), and the target base of the molecular Marker4243 of the strain 6-13 is C (the 801 st base shown in SEQ ID No. 4).
Major QTL locus for amplifying peanut single plant pod numberqPN7Primer pair of linked molecular Marker 4251:
forward primer sequence 5'-ACTGAGTTTACCTACTGCTGAG-3' (shown as SEQ ID NO. 11);
reverse primer sequence 5'-TAGTTGGACGAGGTTGTGAG-3' (shown as SEQ ID NO. 12).
The target base of the molecular Marker4251 of the variety flower is C (the 801 st base shown in SEQ ID No. 5), and the target base of the molecular Marker4251 of the strain 6-13 is T (the 801 st base shown in SEQ ID No. 6).
In the process of molecular labeling amplification, the reaction system for PCR amplification is 50 mu L, and specifically comprises 2 mu L (10-20 ng) of a DNA template, 3 mu L (1.5 mu L each) of a primer pair (0.2 mu M), 10 mu L of dNTP (50 mu M), 25 mu L of KOD buffer solution, 1 mu L of KOD polymerase and ddH 2 O 9 μL。
The PCR amplification reaction program is 94 ℃ for 5 min;98 ℃ for 10 s,60 ℃ for 30 s and 68 ℃ for 1 min, and 35 cycles are total; extension at 68deg.C for 10 min, and preservation at 4deg.C.
Sequencing the PCR amplified product by using an Illumina Hiseq platform, and detecting families with 3 molecular marker sequences identical to those of the strain 6-13 through sequence comparison, wherein the families are marked as AA-type families; the families with the genotype identical to the variety Huayu No. 36 of the 3 molecular markers are marked as aa-type families.
The results show that RIL (F 10 ) 33 families in the population were AA-type, indicating that these families are inqPN7The alleles of the locus are identical to lines 6-13; 97 families are aa type, indicating that the families areqPN7The alleles at the locus were identical to variety number 36 (Table 2).
130 RIL families (33 AA types and 97 AA types) are planted in the test bases of Dongying city (37.31 DEG N,118.62 DEG E) and Qingdao city (36.82 DEG N,120.51 DEG E) in 2020, the planting mode is ridged single-row single-grain film-covered planting, the ridge distance is 0.3 meter, 10 plants are planted in each row, the plant distance is 0.2 meter, the random block design is adopted, and 3 times of repetition are arranged. The test base has uniform fertility, and the conventional field management can be used for timely controlling diseases and insect pests. The middle 8 individual mature pods were harvested in each row and the number of mature pods per plant was counted.
The results showed (Table 2) that aa family individual pods had average values of 19.82 and 17.00 and AA family individual pods had average values of 26.25 and 28.26; there was a very significant difference between individual pod numbers for AA and AA families, indicatingqPN7The different genotypes of the (2) can have different regulatory effects on the number of peanut single-plant pods, and 3 linked molecular markers are used for controlling the number of peanut single-plant podsThe characteristics have a good prediction effect, and are suitable for peanut molecular marker assisted selection breeding.
TABLE 2 differentqPN7Single pod count statistics for genotypic families
Figure 691566DEST_PATH_IMAGE004
Note that: * Represented inp<At the 0.01 level, there was a significant difference between AA and AA families.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> Shandong province peanut institute
<120> linkage molecular marker of peanut single plant pod number major QTL locus qPN7 and application thereof
<160> 12
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 1
gatagccaag aagctagaaa tttagttgca tatatattat atagtgaagt taattggaaa 60
tggaagttat gggcatgtgt tgtgtgcttc taacggctaa gaaggaatgt tcgtattcca 120
tttcaagggg acactctgca ccaagtagga tcagatttta tgtgggcccc accataaggg 180
tgtgaaaatg tgatgaaaaa tctcatgaaa aaggatcatg tggttaaatt attagtgaca 240
aattatgctg cttacacagg tcacactcag atttagaaat aaactaccac aaaaaaataa 300
ttaaaagatg ttaagctaag aatcaaactt ctagaaacaa tttttgtaaa attaagtgct 360
tattgtttcc ctaacataat atcttcctaa acatgctata gccatgtgct tcaccaacag 420
gcaaagaaag tgttattata ttcttagttt tttctaatgt tagatgctct tctaactcaa 480
caaggattta ctaatataaa agcaattttg tagatgttac atgccctaat acaatgtttg 540
tagaaaaata gtagctgtaa acgagcaaca atgagatcat gagcaaattc ttttctttat 600
tacttttcat gagagaccat agttatgttg ggagcaacat ccatccggtt gtcaaacatt 660
tttctttttt tctgtttttt ttggtcaagc ggattggaca gtctccaatc ctaggcatta 720
cacccatgtt ttacacacct acacaacaca ctcacacact accatgggta ctatgggttc 780
ataaacaaca accaacctca actggaattt gaacccggtg cacctccaag caaggcaaac 840
atagttgtca ctaaactaag tcttgcagtg caacattttt ctatttattc ctttttagta 900
gtttgttttt tttttttaaa tttacatgag gtctttttcg cgtaagttgt tgaacaaaca 960
ataagttatc taagaataaa taaaccgggc cacttgacaa cataaaaatc ctcttggagg 1020
aagtaaaata cataaccaga ttaaccaaac tcgactacaa gtatatatat atatatacat 1080
caacaataaa caaaacatat ggggcagtct ctcatgttgg aagcatacct gtttcaaaaa 1140
gcctgtacat tattggtatg caggatttga tttgtgattt gttttattga ctagtatact 1200
tcttcagtca aaaagatcag tgatgatagc gttatcccaa atggcacaca gttcctttat 1260
catgattttt ttgtggaagc taatattatg tcatcaaatg catattgtgg aagttctttt 1320
taactttaac aaggaaatgg cttttgcagg ctggtttgca agggatccta atattttggg 1380
catatactta tgcaagtacc atatatattt caacgcaatc catagctgat gattgttttc 1440
agcaactaaa tgtctgctga gaagcttttt ggaagtatgt tctattactc cattattttt 1500
catcttgagg tcattgttaa catgtccttt ttctttctgt attctttaat tattcttttc 1560
ttttgtaaat gaatattact tagttatttt cttgtgttct c 1601
<210> 2
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 2
gatagccaag aagctagaaa tttagttgca tatatattat atagtgaagt taattggaaa 60
tggaagttat gggcatgtgt tgtgtgcttc taacggctaa gaaggaatgt tcgtattcca 120
tttcaagggg acactctgca ccaagtagga tcagatttta tgtgggcccc accataaggg 180
tgtgaaaatg tgatgaaaaa tctcatgaaa aaggatcatg tggttaaatt attagtgaca 240
aattatgctg cttacacagg tcacactcag atttagaaat aaactaccac aaaaaaataa 300
ttaaaagatg ttaagctaag aatcaaactt ctagaaacaa tttttgtaaa attaagtgct 360
tattgtttcc ctaacataat atcttcctaa acatgctata gccatgtgct tcaccaacag 420
gcaaagaaag tgttattata ttcttagttt tttctaatgt tagatgctct tctaactcaa 480
caaggattta ctaatataaa agcaattttg tagatgttac atgccctaat acaatgtttg 540
tagaaaaata gtagctgtaa acgagcaaca atgagatcat gagcaaattc ttttctttat 600
tacttttcat gagagaccat agttatgttg ggagcaacat ccatccggtt gtcaaacatt 660
tttctttttt tctgtttttt ttggtcaagc ggattggaca gtctccaatc ctaggcatta 720
cacccatgtt ttacacacct acacaacaca ctcacacact accatgggta ctatgggttc 780
ataaacaaca accaacctca gctggaattt gaacccggtg cacctccaag caaggcaaac 840
atagttgtca ctaaactaag tcttgcagtg caacattttt ctatttattc ctttttagta 900
gtttgttttt tttttttaaa tttacatgag gtctttttcg cgtaagttgt tgaacaaaca 960
ataagttatc taagaataaa taaaccgggc cacttgacaa cataaaaatc ctcttggagg 1020
aagtaaaata cataaccaga ttaaccaaac tcgactacaa gtatatatat atatatacat 1080
caacaataaa caaaacatat ggggcagtct ctcatgttgg aagcatacct gtttcaaaaa 1140
gcctgtacat tattggtatg caggatttga tttgtgattt gttttattga ctagtatact 1200
tcttcagtca aaaagatcag tgatgatagc gttatcccaa atggcacaca gttcctttat 1260
catgattttt ttgtggaagc taatattatg tcatcaaatg catattgtgg aagttctttt 1320
taactttaac aaggaaatgg cttttgcagg ctggtttgca agggatccta atattttggg 1380
catatactta tgcaagtacc atatatattt caacgcaatc catagctgat gattgttttc 1440
agcaactaaa tgtctgctga gaagcttttt ggaagtatgt tctattactc cattattttt 1500
catcttgagg tcattgttaa catgtccttt ttctttctgt attctttaat tattcttttc 1560
ttttgtaaat gaatattact tagttatttt cttgtgttct c 1601
<210> 3
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 3
ataaaatata ttttttaatt attttgtatg gaataaaata tttttttaat ttctaaatta 60
ttattgacta tgtagagtat tttatttaaa atttgtgtac atgcatgtat ttacctaagt 120
cattagaaca ttacaaattt ttatagtact cctaacattt tttaagccat ttttttaaaa 180
ttattttgca tagaataaaa tattttttgt agtataattt aaaaaaattg ttaaaaaata 240
ttcatgagct attaaaaatg gacaaaagag tattgtatgg aattcaaatt gataactcat 300
taatatttta aagaagtctc gtaattaaat attttgttag ctttttttaa tgcatgaaat 360
aaaatatatt ttttaatttt taaattatta atttttttaa caattttttt aataagttat 420
taattttttt aataacaatc accctgattt cgaattagag ggtgtgtaca tgtgtatgta 480
attcgaatca aggtgattcg aagttcgaaa caacctaatt cgaattatat aaaaatgtgt 540
tttggttgat tcatgaatta atttttggtt tagcggattt gtgtaatatt ttgctcccct 600
tgccttaata tagtgatttg ccctatgttt aaatactaat tgaagaaaga gaaaaaaaaa 660
ggatggatat ttaaaatatc tcatcaaact tgttattttt caagatcaaa gaaagaagaa 720
gaacgacatt aagcgtaaaa cttcttttgc ccaactttaa ctcttagatg aaagggagcg 780
cactatagag gacgtttttt tgtttgtctt ttttttttgc tttctcttct attcttcatt 840
ggaatatatg caatgcacgg acagaaaaca tctgtaccca actgtatgct ctgcttaccc 900
caatcccaat ctataccctt ccttgataca acaaataaca ttttactgta gttaccatat 960
tctaatatcc aactatcaat acatattatg atcaagatct aataaatgtt tgttatttat 1020
taaccaaaat agcaacagcg gtaggatcaa attttcaaca acaaattctt cagaggggcc 1080
aaaaataatt atttcacccg aaacagcaaa ctcaagtact atagaacatg tagaatagta 1140
aaggtggaaa aaggaatata aaaatcaaca atatgagtta taaggttatg attcacctag 1200
ttccgtatca acttaaagat tatttggaat tgaaatgata acagaaaaaa ttcactctta 1260
aaattttcac tctaaaatat tgttgcgttg aattgggact gcggaagtca caattacaaa 1320
agctcaacta ctatttctta tattcttttt caaccttaac cattgtgtgt gacaacaaaa 1380
gtaaattcca gatataagtt caatgtataa acttttacga aaaagggtta aaaaatttta 1440
ataaattgtt acttaaaaag gcacacaggt gaatttgaaa ctcaatttgg ttgtgacaaa 1500
aaaaaaagac aaacagaaat cacgaaaata aaaaatagta aggtaacagg taacgacttc 1560
tcaagataca aaatgaggac gacagcagtt ggagttgaaa c 1601
<210> 4
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 4
ataaaatata ttttttaatt attttgtatg gaataaaata tttttttaat ttctaaatta 60
ttattgacta tgtagagtat tttatttaaa atttgtgtac atgcatgtat ttacctaagt 120
cattagaaca ttacaaattt ttatagtact cctaacattt tttaagccat ttttttaaaa 180
ttattttgca tagaataaaa tattttttgt agtataattt aaaaaaattg ttaaaaaata 240
ttcatgagct attaaaaatg gacaaaagag tattgtatgg aattcaaatt gataactcat 300
taatatttta aagaagtctc gtaattaaat attttgttag ctttttttaa tgcatgaaat 360
aaaatatatt ttttaatttt taaattatta atttttttaa caattttttt aataagttat 420
taattttttt aataacaatc accctgattt cgaattagag ggtgtgtaca tgtgtatgta 480
attcgaatca aggtgattcg aagttcgaaa caacctaatt cgaattatat aaaaatgtgt 540
tttggttgat tcatgaatta atttttggtt tagcggattt gtgtaatatt ttgctcccct 600
tgccttaata tagtgatttg ccctatgttt aaatactaat tgaagaaaga gaaaaaaaaa 660
ggatggatat ttaaaatatc tcatcaaact tgttattttt caagatcaaa gaaagaagaa 720
gaacgacatt aagcgtaaaa cttcttttgc ccaactttaa ctcttagatg aaagggagcg 780
cactatagag gacgtttttt cgtttgtctt ttttttttgc tttctcttct attcttcatt 840
ggaatatatg caatgcacgg acagaaaaca tctgtaccca actgtatgct ctgcttaccc 900
caatcccaat ctataccctt ccttgataca acaaataaca ttttactgta gttaccatat 960
tctaatatcc aactatcaat acatattatg atcaagatct aataaatgtt tgttatttat 1020
taaccaaaat agcaacagcg gtaggatcaa attttcaaca acaaattctt cagaggggcc 1080
aaaaataatt atttcacccg aaacagcaaa ctcaagtact atagaacatg tagaatagta 1140
aaggtggaaa aaggaatata aaaatcaaca atatgagtta taaggttatg attcacctag 1200
ttccgtatca acttaaagat tatttggaat tgaaatgata acagaaaaaa ttcactctta 1260
aaattttcac tctaaaatat tgttgcgttg aattgggact gcggaagtca caattacaaa 1320
agctcaacta ctatttctta tattcttttt caaccttaac cattgtgtgt gacaacaaaa 1380
gtaaattcca gatataagtt caatgtataa acttttacga aaaagggtta aaaaatttta 1440
ataaattgtt acttaaaaag gcacacaggt gaatttgaaa ctcaatttgg ttgtgacaaa 1500
aaaaaaagac aaacagaaat cacgaaaata aaaaatagta aggtaacagg taacgacttc 1560
tcaagataca aaatgaggac gacagcagtt ggagttgaaa c 1601
<210> 5
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 5
acaagttgaa aacagcaact atagctgacc aagcaaaaga caaacctatc tctaaaccgg 60
ccgaagacaa aatcagatgg gtaaaaaggt atcccgttga ttacaagatt aaatgaccat 120
atatgggtat ttattaccac cttctgctga tccatatttg ttttgctcaa cagaaactag 180
tggagacgcg ttgctcatct tgctacatta acaacttatt tacccaaggg gcaatgcgca 240
aaggcaggaa gaagttgctt acttaggatt agtttcaatg aattgaacta ccacattata 300
ctgagtttac ctactgctga gatgcttagg gatttgtttt tgttaaatta gaaagacttg 360
attccaagac atcttttgtt tttctacagt acaatgattt attggttatg ttacctaaaa 420
attatctatc tttttctata tattagaatc ccgaatttat caaggttttt tggtacagtt 480
ggcataattg tgcatgttga aataggtgac aactaaccat ccaccaatat agctaaactg 540
aacatcccat accttactat actgaacatc cagtttaact agataaaatt caaaacataa 600
caaccaagtg caacatagac aagtactaac gggtaactat ccatgcttag agtaaaaatg 660
aacatctgat tacctatggg aatgaacatc cactttggat cattaaaaat acttgtaaac 720
agtgtatata acaaacaact acgcaccgaa aaactataca gtgaagcatg tagaaataag 780
gtacaacttt attaaactat ctggtatatt acaaaataac caccaaatat actcgttatg 840
cattaagtat acacgaaata aaaatagtaa aatggtttgt tctaactaat gaaaaaaggg 900
ttcttgcaat taataacata tggacttttg caacagctgc cttagaacct tgaaactaac 960
atgaatccta aaaacacaga gatgagaaaa tatacaatct cacaacctcg tccaactaat 1020
cccacttttt gttgaaggag cttaacaaag atatgaaacc accggcttgt tcgggaccat 1080
tcaggccaga aacagcacaa tggtttatat cttgaagtgt gtgcggacga accacctgcc 1140
aaaaaaaaaa gaaaaagtcc aaatagttat gttctaggat agtactatac caaataaaca 1200
atgaacgttg agattaagcc aaagatatac cgggggtgaa ttcttttgtt ttctccgtct 1260
tgaattcttg atggacttct caagggcaga gtcaagcctc ttactctttg gttgcaaatg 1320
cagctttctt tgcataggtc acataaaagt catgcgccat ctgtaactga gcaaaccgca 1380
tttcaactct tggtatctcg tcttcttgta ggttaccatg atctggtaac tgtattttat 1440
attaatccaa aagaaaaatt aaataccatt agttatcaaa tcaaacatga acattataac 1500
tacacaaaag taagctattt tacaatacct catctccaag ctccttctct tcacttccaa 1560
catctgtaac atccgacaac tgcgtggcct aaaattccaa a 1601
<210> 6
<211> 1601
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 6
acaagttgaa aacagcaact atagctgacc aagcaaaaga caaacctatc tctaaaccgg 60
ccgaagacaa aatcagatgg gtaaaaaggt atcccgttga ttacaagatt aaatgaccat 120
atatgggtat ttattaccac cttctgctga tccatatttg ttttgctcaa cagaaactag 180
tggagacgcg ttgctcatct tgctacatta acaacttatt tacccaaggg gcaatgcgca 240
aaggcaggaa gaagttgctt acttaggatt agtttcaatg aattgaacta ccacattata 300
ctgagtttac ctactgctga gatgcttagg gatttgtttt tgttaaatta gaaagacttg 360
attccaagac atcttttgtt tttctacagt acaatgattt attggttatg ttacctaaaa 420
attatctatc tttttctata tattagaatc ccgaatttat caaggttttt tggtacagtt 480
ggcataattg tgcatgttga aataggtgac aactaaccat ccaccaatat agctaaactg 540
aacatcccat accttactat actgaacatc cagtttaact agataaaatt caaaacataa 600
caaccaagtg caacatagac aagtactaac gggtaactat ccatgcttag agtaaaaatg 660
aacatctgat tacctatggg aatgaacatc cactttggat cattaaaaat acttgtaaac 720
agtgtatata acaaacaact acgcaccgaa aaactataca gtgaagcatg tagaaataag 780
gtacaacttt attaaactat ttggtatatt acaaaataac caccaaatat actcgttatg 840
cattaagtat acacgaaata aaaatagtaa aatggtttgt tctaactaat gaaaaaaggg 900
ttcttgcaat taataacata tggacttttg caacagctgc cttagaacct tgaaactaac 960
atgaatccta aaaacacaga gatgagaaaa tatacaatct cacaacctcg tccaactaat 1020
cccacttttt gttgaaggag cttaacaaag atatgaaacc accggcttgt tcgggaccat 1080
tcaggccaga aacagcacaa tggtttatat cttgaagtgt gtgcggacga accacctgcc 1140
aaaaaaaaaa gaaaaagtcc aaatagttat gttctaggat agtactatac caaataaaca 1200
atgaacgttg agattaagcc aaagatatac cgggggtgaa ttcttttgtt ttctccgtct 1260
tgaattcttg atggacttct caagggcaga gtcaagcctc ttactctttg gttgcaaatg 1320
cagctttctt tgcataggtc acataaaagt catgcgccat ctgtaactga gcaaaccgca 1380
tttcaactct tggtatctcg tcttcttgta ggttaccatg atctggtaac tgtattttat 1440
attaatccaa aagaaaaatt aaataccatt agttatcaaa tcaaacatga acattataac 1500
tacacaaaag taagctattt tacaatacct catctccaag ctccttctct tcacttccaa 1560
catctgtaac atccgacaac tgcgtggcct aaaattccaa a 1601
<210> 7
<211> 20
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 7
atttcaaggg gacactctgc 20
<210> 8
<211> 19
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 8
tgtcaagtgg cccggttta 19
<210> 9
<211> 21
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 9
ataacaatca ccctgatttc g 21
<210> 10
<211> 20
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 10
caactccaac tgctgtcgtc 20
<210> 11
<211> 22
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 11
actgagttta cctactgctg ag 22
<210> 12
<211> 20
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 12
tagttggacg aggttgtgag 20

Claims (5)

1. Main effect QTL (quantitative trait locus) site for peanut single plant pod numberqPN7Is characterized in that the linked molecular marker and the main effect QTL locus of peanut single plant pod numberqPN7Is co-located on the 7 th chromosome of the peanut genome and comprises a molecular Marker4240, a molecular Marker4243 and a molecular Marker4251;
the nucleotide sequence of the molecular Marker4240 is shown as SEQ ID NO.1 or SEQ ID NO.2, and the polymorphism is A/G; the nucleotide sequence of Marker4243 is shown as SEQ ID No.3 or SEQ ID No.4, and polymorphism is T/C; the nucleotide sequence of Marker4251 is shown as SEQ ID No.5 or SEQ ID No.6, and polymorphism is C/T.
2. The identification method of the peanut single plant pod number is characterized by comprising the following steps:
extracting genomic DNA of peanut materials to be identified, carrying out PCR (polymerase chain reaction) amplification on the template by using the primer composition, sequencing and analyzing the amplified product, and judging polymorphism of the linkage molecular marker according to a sequencing result;
according to the sequencing result of the peanut material to be identified, if the genotype of the linkage molecular Marker4240 is G, the genotype of the molecular Marker4243 is C, and the genotype of the molecular Marker4251 is T, judging that the number of single plant pods of the peanut material is more than or equal to 20; if the genotype of the chain molecular Marker4240 is A, the genotype of the molecular Marker4243 is T, and the genotype of the molecular Marker4251 is C, judging that the number of single plant pods of the peanut material is less than 20;
the primer composition comprises 6 primers, and the nucleotide sequences of the primers are respectively shown as SEQ ID No. 7-SEQ ID No. 12.
3. The method of claim 2, wherein the PCR amplification reaction procedure is: 94 ℃ for 5 min;98 ℃ for 10 s,60 ℃ for 30 s and 68 ℃ for 1 min, and 35 cycles are total; stopping extending at 68deg.C for 10 min, and preserving at 4deg.C; the PCR amplification reaction system is 50 mu L, and specifically comprises 10-20 ng of DNA template 2 mu L, 1.5 mu L of primer pairs, 10 mu L of dNTPs, 25 mu L of KOD buffer solution, 1 mu L of KOD polymerase and ddH 2 O 9 μL。
4. The application of the primer composition for detecting the linkage molecular marker in the breeding of high single-plant pod number peanut varieties or strains, which is characterized in that the primer composition comprises 6 primers, and the nucleotide sequences of the primers are respectively shown as SEQ ID No. 7-SEQ ID No. 12; the high individual pod number means that the individual pod number is greater than or equal to 20.
5. The application of the primer composition for detecting the linkage molecular marker in high single-plant pod number peanut molecular breeding and culturing transgenic high single-plant pod number peanut or high single-plant pod number peanut germplasm resource improvement is characterized in that the primer composition comprises 6 primers, and the nucleotide sequences of the primers are respectively shown as SEQ ID No. 7-SEQ ID No. 12; the high individual pod number means that the individual pod number is greater than or equal to 20.
CN202111185756.4A 2021-10-12 2021-10-12 Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof Active CN113736910B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111185756.4A CN113736910B (en) 2021-10-12 2021-10-12 Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111185756.4A CN113736910B (en) 2021-10-12 2021-10-12 Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof

Publications (2)

Publication Number Publication Date
CN113736910A CN113736910A (en) 2021-12-03
CN113736910B true CN113736910B (en) 2023-06-27

Family

ID=78726653

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111185756.4A Active CN113736910B (en) 2021-10-12 2021-10-12 Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof

Country Status (1)

Country Link
CN (1) CN113736910B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114438245B (en) * 2022-02-15 2022-10-11 中国农业科学院油料作物研究所 SNP molecular marker linked with peanut bacterial wilt-resistant major QTL locus and application thereof
CN117448474B (en) * 2023-06-29 2024-04-09 河南农业大学 InDel molecular marker related to peanut pod size and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107012246A (en) * 2006-05-25 2017-08-04 孟山都技术有限公司 The authentication method of soybean disease resistance quantitative trait locus and its composition
CN112094937A (en) * 2020-09-27 2020-12-18 中国农业科学院油料作物研究所 SNP molecular marker related to pod and seed size on peanut A06 chromosome and application thereof
CN112626260A (en) * 2021-01-15 2021-04-09 中国农业科学院油料作物研究所 Molecular marker linked with peanut kernel weight major QTL (quantitative trait locus) and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107012246A (en) * 2006-05-25 2017-08-04 孟山都技术有限公司 The authentication method of soybean disease resistance quantitative trait locus and its composition
CN112094937A (en) * 2020-09-27 2020-12-18 中国农业科学院油料作物研究所 SNP molecular marker related to pod and seed size on peanut A06 chromosome and application thereof
CN112626260A (en) * 2021-01-15 2021-04-09 中国农业科学院油料作物研究所 Molecular marker linked with peanut kernel weight major QTL (quantitative trait locus) and application thereof

Also Published As

Publication number Publication date
CN113736910A (en) 2021-12-03

Similar Documents

Publication Publication Date Title
US10064351B2 (en) F. oxysporum F.sp. melonis race 1,2-resistant melons
CN109735652B (en) Wheat stripe rust resistant gene QYr.nwafu-6BL.2 linked KASP molecular marker, primer and application
Olsen et al. Evolutionary genomics of weedy rice in the USA
CN108728575B (en) Major QTL site of brassica napus silique length character, SNP molecular marker and application
CN113736910B (en) Linkage molecular marker of peanut single plant pod number major QTL locus qPN and application thereof
EP2355651B1 (en) Genetic loci associated with northern leaf blight resistance in maize
US20240099256A1 (en) Anthracnose resistant alfalfa plants
CN110684858A (en) Molecular marker of rice long and thin grain type gene and application thereof
CN109688805B (en) Method for producing gray leaf spot resistant maize
CN107667180A (en) Identification and the method for selecting the maize plant resistant to anthrax stem rot
CN104862416B (en) The locus associated with Fusarium ear mold resistance in corn
CN113046462B (en) Molecular marker closely linked with corn cob long-acting QTL, primer and application
EP2486155B1 (en) Methods of quantifying target organisms and creating reniform resistant cotton plants
CN112266975A (en) Primer group and kit for detecting KASP (Kaempferi-N-linked immunosorbent assay) marker related to POD (peroxidase) activity of wheat grains and application of primer group and kit
US9161501B2 (en) Genetic markers for Orobanche resistance in sunflower
US9228241B2 (en) Genetic loci associated with mechanical stalk strength in maize
CN113278723B (en) Composition for analyzing genetic diversity of Chinese cabbage genome segment or genetic diversity introduced in synthetic mustard and application
CN114277175A (en) Rapid and efficient wheat scab-resistant molecular design breeding method
CN108179222B (en) Nucleotide sequence of chorismate mutase related to high yield of rice and application thereof
CN109055370B (en) Straw WSC content gene marker based on Chinese rye 895 and application
CN111961739A (en) Specific primer group for detecting corn Dek41 gene and application thereof
CN112980993B (en) SNP molecular marker linked with major QTL site qPSIIB10 for resisting aspergillus flavus infection of peanuts and application thereof
CN117778616B (en) Wheat grain weight related gene TaSINA molecular marker and application
KR102141703B1 (en) Molecular marker for selecting tomato cultivars resistant to tomato Bacterial wilt and selection method using the same marker
WO2013033234A1 (en) Molecular markers associated with aphid resistance in soybean

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant