CN112106738A - Method for nursing one or two-release autumn tussah to relieve diapause - Google Patents
Method for nursing one or two-release autumn tussah to relieve diapause Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 40
- 230000005058 diapause Effects 0.000 title claims abstract description 24
- 230000000474 nursing effect Effects 0.000 title description 6
- 241000255789 Bombyx mori Species 0.000 claims abstract description 31
- 238000005057 refrigeration Methods 0.000 claims abstract description 28
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 238000009395 breeding Methods 0.000 claims description 20
- 230000001488 breeding effect Effects 0.000 claims description 18
- 238000010792 warming Methods 0.000 claims description 10
- 240000008574 Capsicum frutescens Species 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 abstract description 4
- 230000019617 pupation Effects 0.000 description 11
- 238000003860 storage Methods 0.000 description 11
- 241000382353 Pupa Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 230000008901 benefit Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 229920001661 Chitosan Polymers 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 241000255978 Antheraea pernyi Species 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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Abstract
The invention provides a two-in-one autumn tussah diapause removal seed preservation method, wherein two-in-one autumn tussah cocoons are warmed, bred and raised in a first-in-one silkworm area in spring, and the tussah cocoons are put into a refrigeration house after 90% of the tussah cocoons are pupated; in the freezer: the first stage is 4 days at 18-20 deg.c; the second stage is carried out for 4 days at 14-16 ℃; the third stage is 4 days at 10-12 deg.C; the fourth stage is carried out for 6-10 days at 4-6 ℃; the fifth stage is carried out for 2-7 days at 0-2 ℃; the time of the sixth stage is 6-10 days, and the temperature is 4-6 ℃; the seventh stage is 4 days at 10-12 deg.C; the eighth stage is carried out for 2 days at 14-16 ℃; the ninth stage is 1 day at 18-20 deg.C; then taking out the tussah cocoons from a refrigeration house, and placing the tussah cocoons at room temperature for 15 days to prepare seeds. The emergence rate of the tussah in autumn can reach more than 90 percent, and 4 kilograms of tussah eggs can be produced by 100 kilograms of tussah cocoons.
Description
Technical Field
The application relates to the field of tussah seed production, in particular to a seed preservation method for removing diapause of a bigeneration autumn tussah.
Background
The Henan first-chemical tussah has been popular in Han dynasty, bright and clear, and has more than 2000 years of history from today. In the period of developing into a tripod in 1920-1931, the utilization area of the tussah slope is 1370 mu of ten thousand, 8.61 kg of tussah seeds are raised in the year, and the stocking amount and the cocoon yield are all arranged at the first position of the country. The southern Yangcheng of the main parturient has the prosperous scene that children women can get through the channels and shuttle sound everywhere, and people in vast mountainous areas are mainly based on the silkworm breeding and tussah planting. The Hangul of one basket of silkworms and ten mu of fields fully explains the leading position of the tussah industry in the agricultural production at that time. Then, the first-pass tussah in Henan tends to decline due to the influence of war disorder. After the country is built, the party and the government actively recover and develop the production of the tussah, more than 800 ten thousand mu of tussah is utilized in Henan in 1966, 4.8 thousand kg of seeds are used for silkworm breeding in each year, and 1.2 ten thousand tons of cocoons are produced, thereby creating the highest yield level since the country is built. After the twenty-first century, the tussah silkworm industry in Henan gradually shrinks under the influence of various factors, and the current situation is maintained at the level of 1.5 ten thousand kg of seeds used in the year and 6000 tons of cocoons produced. In recent years, with the continuous deepening and development of the comprehensive utilization research of the tussah, the demand and the price of the tussah cocoons are higher and higher, and the tussah cocoons are often in a state of short supply and short demand. However, the activity of silkworm rearing by silkworm farmers is still low, and the tussah recovers hypodynamia. The fundamental reason for the method is that the labor capacity and the labor intensity are too large, the labor saving and the labor saving are difficult to realize by raising the tussah in spring in Henan at present, the labor saving can be realized only by raising the autumn tussah in a way of one-generation and two-release, the silkworm raising enthusiasm of silkworm raisers is improved, and the Henan tussah is developed rapidly.
The method realizes the breeding of autumn tussah under one-to-two breeding mode, and firstly solves the problem of silkworm seeds. Henan is a first-pass area, the tussah bred in spring is diapaused after pupating, and can not be bred under normal conditions, and northeast is a second-pass area, and the tussah bred in spring is not diapause and can be bred. However, in the middle and late 7 months of autumn tussah rearing in northeast China and in the middle and late 8 months of Henan, the rearing time is one month apart, and the silkworm seeds are too long to be used. Meanwhile, the price of the silkworm seeds in northeast is too high, so that the silkworm seeds in Henan are not easy to accept by silkworm farmers. At present, the Antheraea pernyi strain production in Henan and autumn mainly adopts an artificial light-sensitive diapause relieving method, an artificial low-temperature diapause relieving method and a silkworm pupa hormone injection diapause relieving method. The artificial light-sensitive diapause-relieving method has the defects of large occupied area, long seed production period, ineffective utilization of silkworm seeds produced in the early stage and the later stage, low silkworm pupa emergence rate and the like, and the method has the advantages that about 2 kg of the tussah seeds can be produced by 100 kg of tussah cocoons, and the seed production efficiency is low. The artificial low-temperature diapause-relieving method requires 50 days at the minimum and cannot be applied to actual production. The labor cost of the silkworm pupa hormone releasing diapause method is too high, and the method can only be applied to cell tests. Therefore, it is urgent to find an applicable method for breeding autumn tussah.
Firstly, two tussah cocoons are purchased from northeast China in winter, and the next spring is used for producing seeds and breeding silkworms and the autumn is used for producing seeds. The method has two problems, namely, after the tussah cocoons are finished 6 months and 1 day before the tussah in the spring of the river south, if manual control is not carried out, emergence seed production is carried out 6 months and 15 days before, and emergence seed production of the tussah in the autumn of the river south is carried out 7 months and 25 days before. The low temperature is controlled for about 40 days at 5-8 ℃, the time for starting the low temperature control is very important, the tussah silkworm which may not be pupated is frozen to death in the early stage, and the tussah silkworm pupa development accumulated temperature exceeds 100 ℃ in the late stage, so that the tussah silkworm seed quality is influenced. The second is the problem that the tussah chemical property changes along with the feeding region, the northeast bivoltine tussah is fed to the river south, about 50% of the tussah becomes a normalized tussah in the same year, more than 90% of the special year becomes the normalized tussah, the problem how to change the normalized tussah into the normalized tussah to relieve diapause again is solved urgently in the production of the river south bivoltine autumn tussah.
Disclosure of Invention
In order to solve the problems, the invention provides a two-radiation-per-autumn tussah diapause-removing nursing method, the emergence rate of the tussah in autumn can reach more than 90 percent, and 4 kilograms of tussah eggs can be prepared from 100 kilograms of tussah cocoons.
The object of the invention is achieved in the following way: a two-generation autumn tussah diapause elimination seed preservation method comprises the steps of warming cocoons, producing seeds and raising the two-generation tussah cocoons in a first-generation silkworm area in spring, and putting the tussah cocoons into a refrigeration house after 90% of the tussah cocoons pupate; in the freezer: the first stage is 4 days at 18-20 deg.c; the second stage is carried out for 4 days at 14-16 ℃; the third stage is 4 days at 10-12 deg.C; the fourth stage is carried out for 6-10 days at 4-6 ℃; the fifth stage is carried out for 2-7 days at 0-2 ℃; the time of the sixth stage is 6-10 days, and the temperature is 4-6 ℃; the seventh stage is 4 days at 10-12 deg.C; the eighth stage is carried out for 2 days at 14-16 ℃; the ninth stage is 1 day at 18-20 deg.C; then taking out the tussah cocoons from a refrigeration house, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
The time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 10 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-20 days, and the temperature is 4-6 ℃; 21-27 days 6 months; the temperature is 0-2 ℃; 6 months, 28 days to 7 months, 3 days, and the temperature is 4 to 6 ℃; 7 months and 4-7 days, and the temperature is 10-12 ℃; 7 months and 8-9 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 10 days; taking out tussah cocoons from a refrigeration house in 7 months and 11 days, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
The room temperature conditions were: the temperature is 20-35 ℃.
The Chilli cocoon is from northeast Chilli cocoon in winter of last year.
Has the advantages that: as tussah is raised in the field, the tussah is greatly influenced by factors such as environment, leaf quality, weather conditions and the like, and is easy to develop unevenly, so that 90 percent of tussah is put into a refrigeration house in time after pupating. If all pupations are reached, the development accumulated temperature of early pupation may exceed 100 ℃, and the quality of tussah species is affected.
The temperature of the first stage is 18-20 ℃ in 6 months and 3-6 days, which aims to reduce the development speed of early pupation and ensure that about 10 percent of pupation is normally carried out without pupation because the tussah is not afraid of the low temperature of 0-10 ℃ after pupation. In the ninth stage, the temperature is 18-20 ℃ in 7 months and 10 days, so that the tussah pupa has a transition temperature, and the tussah pupa is prevented from being damaged by temperature change from a cold storage to an external temperature. The temperature is changed according to the requirement, if the temperature is not well controlled, the emergence rate of tussah can be obviously reduced, and the seed production benefit is influenced.
The breeding method for removing diapause of the autumn oak by one-half-release completely overcomes the defects and shortcomings of other breeding methods for removing diapause, the emergence rate of the oak in autumn can reach more than 90%, 4 kilograms of eggs can be produced from 100 kilograms of tussah cocoons, various indexes of the tussah breeding in autumn in Henan are reached, the economic benefit is obvious, and the application prospect is wide.
Detailed Description
The two-in-one autumn tussah diapause elimination seed preservation method comprises the steps of warming cocoons, producing seeds and raising the two-in-one autumn tussah in a first-in-one cocoon zone in spring, and putting the tussah cocoons into a cold storage in time by a seed production unit after 90% of the tussah cocoons are pupated; in the freezer: the first stage is 4 days at 18-20 deg.c; the second stage is carried out for 4 days at 14-16 ℃; the third stage is 4 days at 10-12 deg.C; the fourth stage is carried out for 6-10 days at 4-6 ℃; the fifth stage is carried out for 2-7 days at 0-2 ℃; the time of the sixth stage is 6-10 days, and the temperature is 4-6 ℃; the seventh stage is 4 days at 10-12 deg.C; the eighth stage is carried out for 2 days at 14-16 ℃; the ninth stage is 1 day at 18-20 deg.C; then taking out the tussah cocoons from a refrigeration house, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
As tussah is raised in the field, the tussah is greatly influenced by factors such as environment, leaf quality, weather conditions and the like, and is easy to develop unevenly, so that 90 percent of tussah is put into a refrigeration house in time after pupating. If all pupations are reached, the development accumulated temperature of early pupation may exceed 100 ℃, and the quality of tussah species is affected.
The first stage temperature is 18-20 deg.c, and aims at lowering the growth speed of early pupation and making about 10% of pupation be normal, because the temp. of 0-10 deg.c is not affected after the tussah pupation. The temperature of the ninth stage is 18-20 ℃, so that the tussah pupa has a transition temperature, and the temperature is prevented from causing damage to the tussah pupa due to sudden temperature change from the cold storage to the external temperature. The temperature is changed according to the requirement, if the temperature is not well controlled, the emergence rate of tussah can be obviously reduced, and the seed production benefit is influenced.
Because the environmental conditions, particularly the temperature and the humidity, are different every year, the cocooning and pupating time of the tussah also changes, sometimes the cocoons are advanced by 28 days in 5 months, sometimes the cocoons are delayed to 8 days in 6 months, the maximum time difference between different years is about 10 days, and for the convenience of actual production and operation, the 3 days in 6 months is selected as the representative time for placing the tussah cocoons into a cold storage. The time for putting the tussah cocoons into the refrigeration house can be advanced or delayed according to the actual condition of the current year, the 5 th time period can be shortened if the time for putting the tussah cocoons into the refrigeration house is reduced after 6 months and 3 days, and the 4 th time period or the 6 th time period can be prolonged if the time for putting the tussah cocoons into the refrigeration house is increased before 6 months and 3 days.
The time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 10 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-20 days, and the temperature is 4-6 ℃; 21-27 days 6 months; the temperature is 0-2 ℃; 6 months, 28 days to 7 months, 3 days, and the temperature is 4 to 6 ℃; 7 months and 4-7 days, and the temperature is 10-12 ℃; 7 months and 8-9 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 10 days; taking out tussah cocoons from a refrigeration house in 7 months and 11 days, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
The time for taking the tussah cocoons out of the refrigeration house is about 7 months and 11 days, the tussah cocoons are taken out of the refrigeration house within 7 months and 11 days, the tussah cocoons begin to feather out to produce the moths and seeds within 15 days, namely, the 7 months and 25 days, the time is about 10 days, namely, the seeds production within 8 months and 4-5 days is finished, the time for hatching the tussah eggs in autumn is 9 days, namely, the optimal silkworm-producing time for the tussah in autumn of Henan is about 14 days, therefore, the tussah cocoons are taken out of the refrigeration house within 7 months and 11 days, and the tussah cocoons are placed in the refrigeration house after being taken out of the refrigeration house.
The room temperature conditions were: the temperature is 20-35 ℃. The indoor temperature is normally 20-35 ℃ from the middle and the last 7 months to the last 8 months, particularly, the seed production houses require good ventilation and cooling equipment under good conditions, so the high temperature does not exceed 35 ℃.
The Chilli cocoon is from northeast Chilli cocoon in winter of last year.
The present invention is described in detail below with reference to specific embodiments, it should be noted that the embodiments are only used for further illustration of the present invention, and should not be construed as limiting the scope of the present invention, and those skilled in the art can make modifications and adaptations of the present invention based on the above-mentioned disclosure.
Comparative example 1
Two kinds of cocoon are purchased from northeast of China in 2015 year, and normal cocoon warming, seed production and breeding are carried out in Henan in 2016 year in spring. 10 jin of each unit of self-fed Chissah cocoons are stored at room temperature by the Federal scientific research institute of silkworm industry in Henan province at 6 months and 4 days in 2016, emergence begins at 20 days in 6 months, and the emergence rate is 46%. Meanwhile, putting another 500 jin of cocoons into a refrigeration house, wherein the temperature is 18-20 ℃ in 6 months and 4-7 days, the temperature is 6-8 ℃ in 6 months and 8 days-7 months and 9 days, the temperature is 18-20 ℃ in 7 months and 10 days, the cocoons are taken out from the refrigeration house in 7 months and 11 days, the cocoons begin to eclose in 7 months and 25 days, and the eclosion rate is 58%. The result shows that the effect of relieving diapause at constant temperature is not good, and the research of the method of relieving diapause at variable temperature is started from this point. The room temperature is 20-35 ℃.
Comparative example 2
The method is characterized in that the method comprises the steps of purchasing two kinds of seed cocoons from northeast in 2017 in winter, normally warming the cocoons in Henan in 2018 in spring, and carrying out seed production and breeding. The method comprises the steps of purchasing 2000 jin of Chitosan cocoons in the same year from an tussah stock ground of a southern river store in Henan province, Suzhou county, Henan province, 2 months and 6 months in 2018, directly placing the Chitosan cocoons into a 6-8-DEG cold storage for refrigeration, taking the Chitosan cocoons out of the cold storage in 10 months after 7 months, starting emergence in 25 days, and enabling the emergence rate to be only 15%.
Example 1
The method is characterized in that the method comprises the steps of purchasing two kinds of seed cocoons from northeast in 2017 in winter, normally warming the cocoons in Henan in 2018 in spring, and carrying out seed production and breeding. After 90% of tussah cocoons pupate in 3 days at 6 months, the scientific research institute of silkworm industry in Henan province buys the two cocoons fed in the current year in time, and puts the cocoons into a cold storage. The time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 10 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-20 days, and the temperature is 4-6 ℃; 21-27 days 6 months; the temperature is 0-2 ℃; 6 months, 28 days to 7 months, 3 days, and the temperature is 4 to 6 ℃; 7 months and 4-7 days, and the temperature is 10-12 ℃; 7 months and 8-9 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 10 days; the tussah cocoons are taken out from the refrigeration house in 7 months and 11 days, and can be placed at room temperature for 15 days to produce seeds, the emergence rate is 92 percent, the tussah seeds produced by 100 jin cocoons are 4.1 kg, and the effect is ideal.
Comparing comparative example 2 with example 1, the reason why the diapause pupa rate of comparative example 1 is high is analyzed: firstly, the tussah in the current year is in 5-year age, the rainy days are more, the illumination is insufficient, and the diapause pupa rate is high. Secondly, temperature-changing measures are not adopted, and diapause of the diapause pupae is not relieved.
Example 2
The method comprises the steps of normally warming cocoons in Henan in 2018 spring from two seed cocoons purchased from northeast in 2017, and carrying out seed production and breeding. A two-season releasing method for nursing the young autumn tussah to eliminate diapause includes such steps as 6-3 days, making the tussah pupate by 90%, purchasing the young tussah in time, and cold storage. The time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 13 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-22 days, and the temperature is 4-6 ℃; 6 months, 23-27 days; the temperature is 0-2 ℃; 6 months, 28 days to 7 months, 6 days, and the temperature is 4 to 6 ℃; 7 months and 7-10 days, and the temperature is 10-12 ℃; 7 months, 11-12 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 13 days; the tussah cocoons are taken out from the refrigeration house in 7 months and 14 days, and can be placed at room temperature for 15 days to produce seeds, the emergence rate is 92 percent, the yield of each 100 jin of cocoons is 4.2 kg, and the effect is ideal.
Example 3
The method comprises the steps of normally warming cocoons in Henan in 2018 spring from two seed cocoons purchased from northeast in 2017, and carrying out seed production and breeding. A two-season releasing method for nursing the young autumn tussah to eliminate diapause includes such steps as 6-3 days, making the tussah pupate by 90%, purchasing the young tussah in time, and cold storage. The time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 12 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-24 days, and the temperature is 4-6 ℃; 25-29 days in 6 months; the temperature is 0-2 ℃; 6 months and 30 days to 7 months and 5 days, and the temperature is 4 to 6 ℃; 7 months, 6-9 days, and the temperature is 10-12 ℃; 7 months, 10-11 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 12 days; the tussah cocoons are taken out from the refrigeration house in 7 months and 13 days, and can be placed at room temperature for 15 days to produce seeds, the emergence rate is 93.2 percent, the yield of each 100 jin of cocoons is 4.1 kg, and the effect is ideal.
Example 4
The method comprises the steps of normally warming cocoons in Henan in 2019 spring from two seed cocoons purchased from northeast in 2018, and carrying out seed production and breeding. A two-season releasing method for nursing the young autumn tussah to eliminate diapause includes such steps as 6 months and 1 day, making the tussah pupate by 90%, purchasing the young tussah in time, and cold storage. The time for putting the ice house into the ice house is from 6 months 1 days to 7 months 12 days: the temperature is as follows: 6 months and 1-4 days, and the temperature is 18-20 ℃; 6 months and 5-8 days, and the temperature is 14-16 ℃; 6 months, 9-12 days, and the temperature is 10-12 ℃; 6 months, 13-21 days, and the temperature is 4-6 ℃; 22-25 days in 6 months; the temperature is 0-2 ℃; 26 days in 6 months to 5 days in 7 months, and the temperature is 4 to 6 ℃; 7 months, 6-9 days, and the temperature is 10-12 ℃; 7 months, 10-11 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 12 days; the tussah cocoons are taken out from the refrigeration house in 7 months and 13 days, and can be placed at room temperature for 15 days to produce seeds, the emergence rate is 91.8 percent, the yield of each 100 jin of cocoons is 4.0 kg, and the effect is ideal.
Example 5
The method comprises the steps of normally warming cocoons in Henan in 2019 spring from two seed cocoons purchased from northeast in 2018, and carrying out seed production and breeding. After 90% of tussah cocoons pupate in 1 day at 6 months, the scientific research institute of silkworm industry in Henan province buys the two cocoons fed in the current year in time, and puts the cocoons into a cold storage. The time for putting the ice house into the ice house is from 6 months 1 days to 7 months 10 days: 6 months and 1-4 days, and the temperature is 18-20 ℃; 6 months and 5-8 days, and the temperature is 14-16 ℃; 6 months, 9-12 days, and the temperature is 10-12 ℃; 6 months, 13-20 days, and the temperature is 4-6 ℃; 21-26 days 6; the temperature is 0-2 ℃; 27 days in 6 months to 3 days in 7 months at the temperature of 4-6 ℃; 7 months and 4-7 days, and the temperature is 10-12 ℃; 7 months and 8-9 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 10 days; the tussah cocoons are taken out from the refrigeration house in 7 months and 11 days, and can be placed at room temperature for 15 days to produce seeds, the emergence rate is 92.6 percent, the yield of each 100 jin of cocoons is 4.2 kg, and the effect is ideal.
While the preferred embodiments of the present invention have been described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (4)
1. A two-release autumn tussah diapause eliminating seed preservation method is characterized in that two-release autumn tussah cocoon warming, seed production and breeding are carried out in a first-release silkworm area in spring, and the method comprises the following steps: after 90% of the tussah cocoons pupate, putting the tussah cocoons into a refrigeration house; in the freezer: the first stage is 4 days at 18-20 deg.c; the second stage is carried out for 4 days at 14-16 ℃; the third stage is 4 days at 10-12 deg.C; the fourth stage is carried out for 6-10 days at 4-6 ℃; the fifth stage is carried out for 2-7 days at 0-2 ℃; the time of the sixth stage is 6-10 days, and the temperature is 4-6 ℃; the seventh stage is 4 days at 10-12 deg.C; the eighth stage is carried out for 2 days at 14-16 ℃; the ninth stage is 1 day at 18-20 deg.C; then taking out the tussah cocoons from a refrigeration house, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
2. The method for breeding the oak silkworm by using the chemical double-release autumn culture as claimed in claim 1, wherein the method comprises the following steps: the time for putting the ice house into the ice house is from 6 months and 3 days to 7 months and 10 days: the temperature is as follows: 6 months and 3-6 days at 18-20 ℃; 6 months and 7-10 days, and the temperature is 14-16 ℃; 6 months, 11-14 days, and the temperature is 10-12 ℃; 6 months and 15-20 days, and the temperature is 4-6 ℃; 21-27 days 6 months; the temperature is 0-2 ℃; 6 months, 28 days to 7 months, 3 days, and the temperature is 4 to 6 ℃; 7 months and 4-7 days, and the temperature is 10-12 ℃; 7 months and 8-9 days, and the temperature is 14-16 ℃; the temperature is 18-20 ℃ in 7 months and 10 days; taking out tussah cocoons from a refrigeration house in 7 months and 11 days, and placing the tussah cocoons at room temperature for 15 days to prepare seeds.
3. The method for breeding the oak silkworm by using the chemical double-release autumn culture as claimed in claim 1, wherein the method comprises the following steps: the room temperature conditions were: the temperature is 20-35 ℃.
4. The method for breeding the oak silkworm by using the chemical double-release autumn culture as claimed in claim 1, wherein the method comprises the following steps: the Chilli cocoon is from northeast Chilli cocoon in winter of last year.
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