CN108477082A - The instant termination of diapause of silkworm heat shock and the method for refrigeration delay hatching - Google Patents
The instant termination of diapause of silkworm heat shock and the method for refrigeration delay hatching Download PDFInfo
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- CN108477082A CN108477082A CN201810195942.8A CN201810195942A CN108477082A CN 108477082 A CN108477082 A CN 108477082A CN 201810195942 A CN201810195942 A CN 201810195942A CN 108477082 A CN108477082 A CN 108477082A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 222
- 230000012447 hatching Effects 0.000 title claims abstract description 72
- 230000005058 diapause Effects 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000035939 shock Effects 0.000 title claims abstract description 26
- 238000005057 refrigeration Methods 0.000 title claims abstract description 23
- 241000257303 Hymenoptera Species 0.000 claims abstract description 17
- 238000005554 pickling Methods 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000000926 separation method Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000017448 oviposition Effects 0.000 claims abstract description 5
- 235000013601 eggs Nutrition 0.000 claims description 32
- 239000004698 Polyethylene Substances 0.000 claims description 5
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- 238000003860 storage Methods 0.000 claims description 2
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- 238000004519 manufacturing process Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000282376 Panthera tigris Species 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- 229920006266 Vinyl film Polymers 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 235000021307 Triticum Nutrition 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a kind of instant termination of diapause of silkworm heat shock and the methods of refrigeration delay hatching comprising following steps：S1, the processed silkworm seed of pickling is hastened the hatching of silkworms to hatching and raise to cocooing；S2, it collects and changes the female male silk moth of moth simultaneously, separation of copulating moth after 4 5h that mate, and female moth is put in silkworm and is connected on paper, oviposition；S3, after oviposition, the silkworm for being placed with silkworm seed connects paper and protects in insulating box, after preset time, hot water Heat thermostability 5s, points of three parts processing after drying；After S4, first part of silkworm seed hasten the hatching of silkworms, raising of collecting ants；Second part of silkworm seed hastens the hatching of silkworms, and after silkworm seed point blueness, is placed in middle database after 2h, wraps silkworm seed with film, be put in freezer, every the silkworm seed that 3 days take out predetermined amount, after the silkworm seed of taking-up is placed in middle database 2h, is put under 25 DEG C of environment raising of collecting ants；After third part silkworm seed is placed in middle database 6h, silkworm seed is wrapped with film in freezer, after 30 days, a part of silkworm seed was taken out every 5 days, silkworm seed is first placed in middle database for 24 hours, then hastens the hatching of silkworms and raising of collecting ants.
Description
Technical field
The invention belongs to biotechnology, bioengineering field, it is related to a kind of instant termination of diapause of silkworm heat shock and refrigeration is prolonged
The method hatched late.
Background technology
Silkworm is commonly called as silkworm, is a kind of spinning insect using mulberry leaf as the Lepidoptera of foodstuff, with very high economic value,
Belong to invertebrate, Arthropoda Bombycidae silkworm moth category Silkworm.Silkworm all one's life is by 4 ovum, larva, pupa, adult forms
With the stage of development entirely different on physiological function, the different parts and its metabolin of each stage of development have extensive medicine
With value.
Silkworm is there are one important being of color physiological phenomenon, i.e., the algebraically occurred in 1 year.Univoltine Silkworm varieties are only undergone
One epoch, the ovum given birth to enter a very long duration of diapause, until second year terminate under the conditions of suitable temperature humidity it is stagnant
It educates and germinates；In 1 year occur in the natural environment for Bivoltine silkworm kind two generations, can be controlled by artificial termination of diapause numerous
Grow algebraically；Three generations or more occurs in 1 year for polyvoltinism cultivated silkworm breed variety；Non- Diapause polyvoltine kind does not have duration of diapause.Bivoltine man
Silkworm varieties larval growth phase, silk cocoon size are changed between one between polyvoltine kind, and constitution, characteristics of cocoons and cocoon filament etc. are well suited for silkworm
Needed for industry production, so sericultural production generally uses bivoltine Diapausing egg, two changes that the production traits is excellent, wide in variety are formd
Property varietal population.
In recent years, with the rise of agriculture Carnival in all parts of the country, science popularization base, Agricultural Science and Technology Park etc., silkworm culture
More and more be applied to relevant theme venue (such as：Beijing Agriculture Carnival, Guangxi Yulin agricultural Carnival, Jiang Suyang
River agricultural Carnival etc.).In order to fully show the silkworm culture in China, construction unit in addition to buying common silkworm egg on the market
Outside, it is also necessary to buy special silkworm egg resource from each breeding agency and be shown.Since silkworm is a kind of living animal, a generation
Time there was only 1 wheat harvesting period, in addition agriculture Carnival most at present is all to open garden operation the anniversary, it is therefore desirable to continual
Buy special germ plasm resource.Relative to egg for silk production, these Special Resources not only spend the fund of great number, can't be according to need
It to buy at any time.These problems generally existing in current silkworm breeding production, if unit of operation oneself can reserve seed for planting,
A large amount of purchase fund can not only be saved, moreover it is possible to meet the needs of oneself garden anniversary displaying.Since there are diapause to show for silkworm seed
As the method for the upper termination of diapause of production at present mainly has low-temperature treatment, pickling processing, hydrogen peroxide treatment etc..Low-temperature treatment one
As need 3 months or more 5 DEG C of deepfreezes, therefore the requirement of incubation time cannot be met.At instant pickling and hydrogen peroxide
Reason needs equipment, operation standard and stringent the technology requirement of profession, how to utilize the instant termination of diapause of easy method, becomes
One problem.Simultaneously as the displaying function that garden is special, it is desirable that silkworm each period all exists simultaneously, therefore does not require nothing more than
The instant termination of diapause of silkworm seed, it is necessary to which how research delays the hatching of termination of diapause silkworm seed, and silkworm seed is made to hatch in batches, meets different
The simultaneous bandwagon effect of silkworm of silkworm age.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of instant termination of diapause of silkworm heat shock and refrigeration delay hatching
Method by the instant termination of diapause of heat shock technology, and passes through deepfreeze technology using cultivated silkworm diapause breed variety as original material
Delay hatching, realizing the incubation time of silkworm, while obtaining from silkworm seed,--5 age of newly-hatched silkworm -4 age of -3 age of -2 age of -1 age-cocoons each
The being in period shows for garden, reaches good bandwagon effect.
The invention is realized in this way：
A kind of instant termination of diapause of silkworm heat shock and the method for refrigeration delay hatching comprising following steps：
S1, by the processed silkworm seed of pickling in the climate box environment that temperature is 24~25.5 DEG C, dry and wet difference is 1.5~2 DEG C
In hasten the hatching of silkworms to hatching and raise to cocooing；
S2, the female male silk moth collected at a temperature of 25 DEG C of protection while changing moth, mate under conditions of 25 DEG C, room light
Separation of copulating moth after 4-5h, and female moth is put in silkworm and is connected on paper, it lays eggs, lays eggs 6~8 hours in dark environment；
S3, after oviposition, be placed with silkworm seed silkworm connect paper protect in 25 DEG C of insulating box, after preset time, silkworm is connected
The hot water Heat thermostability 5s of paper preset temperature, point three parts of processing after drying；
The silkworm seed that S4, first part of instant Heat thermostability are crossed, it is 24~25.5 DEG C, poor 1.5~2 DEG C of the gas of dry and wet to be put in temperature
After time case is hastened the hatching of silkworms, raising of collecting ants；The silkworm seed that second part of instant Heat thermostability is crossed, it is 24~25.5 DEG C, dry and wet to be put in temperature
Poor 1.5~2 DEG C of climate box hastens the hatching of silkworms, and after silkworm seed point blueness, is placed in middle database after 2h, wraps silkworm seed with film, put
In freezer, took out the silkworm seed of predetermined amount in batches every 3 days and be put into 25 DEG C of rings after the silkworm seed of taking-up is placed in middle database 2h
It collects ants under border raising；After the silkworm seed that the instant Heat thermostability of third part is crossed is placed in middle database 6h, refrigerate in freezer, in freezer
It is middle to be wrapped silkworm seed with film, after 30 days, took out the silkworm seed of predetermined amount in batches every 5 days, the silkworm seed of taking-up is first placed in centre
Library for 24 hours, then be put in temperature be 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet is hastened the hatching of silkworms and raising of collecting ants.
Preferably, in step S4, after the silkworm seed point blueness after second part of instant Heat thermostability when total preservation in freezer
Between 3~20 days.
Preferably, in step S4, storage total time 30 in freezer of silkworm seed after the instant Heat thermostability of third part~
50 days.
Preferably, in step s 4, the silkworm seed that second part of instant Heat thermostability is crossed takes out one from freezer, every 3 days
It is secondary, 6 taking-ups are divided into, the silkworm seed taken out every time is the 1/6 of the silkworm seed total amount that second part of instant Heat thermostability is crossed；Third part is instant
The silkworm seed that Heat thermostability is crossed took out once from freezer every 5 days, was divided into 4 taking-ups, and the silkworm seed taken out every time is third part
The 1/4 of the silkworm seed total amount that instant Heat thermostability is crossed.
Preferably, the indoor luminous intensity is 50~300Lux.
Preferably, the preset time is 12-24h, and the preset temperature is 53.1 ± 0.5 DEG C.
Preferably, the temperature of the middle database is 15-16 DEG C.
Preferably, the temperature of the freezer is 5 ± 0.5 DEG C.
Preferably, the freezer is refrigerator.
Preferably, the film is polyethylene film.
Compared with prior art, the invention has the advantages that：
1. the present invention provides a kind of easy releasing silkworm egg diapause method immediately, in no professional pickling termination of diapause equipment
Under operating technology, the instant termination of diapause for exempting from pickling is realized by heat shock, does not have particular/special requirement, method letter to equipment and technology
Just, easy to operate；
2. the present invention after heat shock releases silkworm egg diapause immediately using deepfreeze delay hatching technique realize silkworm with
When hatching and hatch by several times in batches, while obtaining from silkworm seed--5 age of newly-hatched silkworm -4 age of -3 age of -2 age of -1 age-work in each period of cocooing
Object is shown for garden, reaches good bandwagon effect, solves current agricultural Carnival, Agricultural Park silkworm theme is faced
While show the problems of different silkworm age silkworms, save the purchase kind expense of great number, can realize the continual exhibition of the anniversary of silkworm egg
Show effect, is suitble to wide and practical promote；
3. the instant termination of diapause of silkworm heat shock of the present invention and the method for refrigeration delay hatching, method is simple to operation, right
The production and management of silkworm can need artificially to be adjusted according to displaying plan, the germplasm special without continual purchase
Resource simplifies purchase and the preservation work of resource, reduces cost.
Description of the drawings
Fig. 1 is the instant termination of diapause of silkworm heat shock of the present invention and the flow chart of the method for refrigeration delay hatching；
Fig. 2 is the state change that the instant termination of diapause of silkworm heat shock and refrigeration of the invention postpone silkworm in hatching process.
Specific implementation mode
Below with reference to the attached drawing exemplary embodiment that the present invention will be described in detail, feature and aspect.It is identical attached in attached drawing
Icon note indicates functionally the same or similar element.Although the various aspects of embodiment are shown in the accompanying drawings, unless special
It does not point out, it is not necessary to attached drawing drawn to scale.
Diapause silkworm is just very long automatically into one after breeding within 1 year one or two under field conditions (factors) from generation to generation
Duration of diapause will show that the silkworm of its different silkworm age needs continuous purchase germ plasm resource, expensive, and preserve cost
Height, therefore the method that the present invention provides a kind of instant termination of diapause of silkworm heat shock and refrigeration delay hatching are using easy heat shock
When termination of diapause after using deepfreeze delay hatching, to obtain cocoon from silkworm seed--5 age of newly-hatched silkworm -4 age of -3 age of -2 age of -1 age -
The being in each period is shown comprising following steps for garden：
S1, by the processed silkworm seed of pickling in the climate box environment that temperature is 24~25.5 DEG C, dry and wet difference is 1.5~2 DEG C
In hasten the hatching of silkworms to hatching and raise to cocooing；
S2, the female male silk moth collected at a temperature of 25 DEG C of protection while changing moth, mate under conditions of 25 DEG C, room light
Separation of copulating moth after 4-5h, and female moth is put in silkworm and is connected on paper, it lays eggs, lays eggs 6~8 hours in dark environment；
S3, after oviposition, be placed with silkworm seed silkworm connect paper protect in 25 DEG C of insulating box, after 12-24h, silkworm is connected into paper
With 53.1 DEG C ± 0.5 DEG C of hot water Heat thermostability 5s, the diapause that silkworm connects silkworm egg on paper is released, point three parts of processing after drying；
The silkworm egg that S4, first part of instant Heat thermostability are crossed, it is 24~25.5 DEG C, poor 1.5~2 DEG C of dry and wet to be directly put in temperature
Climate box hastened the hatching of silkworms after, raising of collecting ants；The silkworm egg that second part of instant Heat thermostability is crossed, be put in temperature be 24~25.5 DEG C,
Poor 1.5~2 DEG C of the climate box of dry and wet hastens the hatching of silkworms, and after silkworm seed point blueness, is placed in 15-16 DEG C of middle database after 2h, with poly- second
Alkene film wraps silkworm seed, is put in 5 ± 0.5 DEG C of freezer, takes out silkworm seed in batches, and a part of silkworm seed was taken out every 3 days,
After the silkworm seed of taking-up is placed in 15-16 DEG C of middle database 2h, raising of collecting ants is put under 25 DEG C of environment；The instant Heat thermostability mistake of third part
Silkworm egg be placed in 15-16 DEG C of middle database 6h after, refrigerate in 5 ± 0.5 DEG C of freezer, polyethylene film is used in freezer
Silkworm egg is wrapped；After 30 days, silkworm seed is taken out in batches, a part of silkworm seed was taken out every 5 days, and the silkworm seed of taking-up is first placed in 15-16
DEG C middle database for 24 hours, then be put in temperature be 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet is hastened the hatching of silkworms and feeding of collecting ants
It supports.
In the present embodiment, preservation total time of the silkworm egg that second part of instant Heat thermostability is crossed in freezer is 3~20
It, preservation total time of the silkworm egg that the instant Heat thermostability of third part is crossed in freezer is 30~50 days.
Embodiment 1
S1, by the processed cultivated silkworm diapause breed variety q- quails spot silkworm of the pickling bought from silkworm egg research institute of the Chinese Academy of Agricultural Sciences
Ovum is hastened the hatching of silkworms in the climate box environment that temperature is 24~25.5 DEG C, dry and wet difference is 1.5~2 DEG C to hatching and raise to cocooing；
S2, the female male silk moth for collecting while changing moth at a temperature of 25 DEG C of kind cocoon protection, under conditions of 25 DEG C, room light
Separation of copulating moth after mating 4-5h, and female moth is put in silkworm and is connected on paper, it lays eggs, lays eggs 6-8 hours in dark environment；
S3, after abounding with ovum, be placed with silkworm seed silkworm connect paper protect in 25 DEG C of insulating box, after 12-24h, silkworm is connected
53.1 DEG C of hot water Heat thermostability 5s of paper, instant termination of diapause, point three parts of processing after drying；
The silkworm seed that S4, first part of instant Heat thermostability are crossed, it is 24~25.5 DEG C, poor 1.5~2 DEG C of dry and wet to be directly put in temperature
Climate box hastened the hatching of silkworms after, raising of collecting ants, hatching rate is about 62%；The silkworm seed that second part of instant Heat thermostability is crossed, directly puts
In temperature be 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet hasten the hatching of silkworms, after silkworm seed point blueness, be placed in 15-16 DEG C
In middle database after 2h, silkworm seed is wrapped with polyethylene film, is put in 5 DEG C of refrigerator, 1/6 silkworm seed was taken out every 3 days, is divided into
6 taking-ups after the silkworm seed of taking-up is placed in 15-16 DEG C of middle database 2h, is put under 25 DEG C of environment and prepares raising of collecting ants, hatching rate is such as
Shown in table 1, hatching rate is reduced with extending with for the holding time in refrigerator, and the 6th hatching rate is 45.6%；Third
It after part silkworm seed that Heat thermostability is crossed immediately is placed in 15-16 DEG C of middle database 6h, refrigerates in 5 DEG C of refrigerator, with poly- in refrigerator
Vinyl film wraps silkworm seed；After 30 days, 1/4 silkworm seed was taken out every 5 days, is divided into the taking-up of 4 batches, and the silkworm seed of taking-up is first placed in
In 15-16 DEG C of middle database for 24 hours, then it is put in that temperature is 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet hastened the hatching of silkworms simultaneously
It collects ants raising, hatching rate is as shown in table 1, and with the extension of refrigerating chamber time, hatching rate is in the trend slightly risen, highest hatching
Rate is 88.5%.
The hatching rate of three parts of different silkworm egg processing after 1 cultivated silkworm diapause breed variety q- quail spot silkworm seed Heat thermostabilities of table
Embodiment 2
S1, the processed cultivated silkworm diapause breed variety Ze- tiger spots of pickling that will be bought from silkworm egg research institute of the Chinese Academy of Agricultural Sciences
Silkworm seed is 24~25.5 DEG C, is hastened the hatching of silkworms in the climate box environment of poor 1.5~2 DEG C of dry and wet to hatching and raise to cocooing in temperature；
S2, the female male silk moth for collecting while changing moth at a temperature of 25 DEG C of kind cocoon protection, under conditions of 25 DEG C, room light
Separation of copulating moth after mating 4-5h, and female moth is put in silkworm and is connected on paper, it lays eggs, lays eggs 6-8 hours in dark environment,；
S3, after abounding with ovum, silkworm is connected paper with 53.1 DEG C of heat by kind silkworm protection in 25 DEG C of insulating box after 12-24h
Water Heat thermostability 5s, instant termination of diapause, point three parts of processing after drying；
The silkworm seed that S4, first part of instant Heat thermostability are crossed, it is 24~25.5 DEG C, poor 1.5~2 DEG C of dry and wet to be directly put in temperature
Climate box hastened the hatching of silkworms after, raising of collecting ants, hatching rate is about 85%；The silkworm seed that second part of instant Heat thermostability is crossed, directly puts
In temperature be 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet hasten the hatching of silkworms, after silkworm seed point blueness, be placed in 15-16 DEG C
In middle database after 2h, silkworm seed is wrapped with polyethylene film, is put in 5 DEG C of refrigerator, 1/6 silkworm seed was taken out every 3 days, altogether
Point 6 batches are taken out, and after the silkworm seed of taking-up is placed in 15-16 DEG C of middle database 2h, are put under 25 DEG C of environment raising of collecting ants, hatching rate is such as
Shown in table 2, hatching rate is reduced with extending with for the holding time in refrigerator, and the 6th hatching rate is 42.5%；Third
It after part silkworm seed that Heat thermostability is crossed immediately is placed in 15-16 DEG C of middle database 6h, refrigerates in 5 DEG C of refrigerator, with poly- in refrigerator
Vinyl film wraps silkworm seed；After 30 days, 1/4 silkworm seed was taken out every 5 days, is divided into the taking-up of 4 batches, and the silkworm seed of taking-up is first placed in
In 15-16 DEG C of middle database for 24 hours, then it is put in that temperature is 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet hastened the hatching of silkworms simultaneously
It collects ants raising, with the extension of refrigerating chamber time, hatching rate is in the trend slightly risen, and highest hatching rate is 90.5%.
The hatching rate of three parts of different silkworm egg processing after 2 cultivated silkworm diapause breed variety Ze- tiger spot silkworm seed Heat thermostabilities of table
Conventional method uses that first silkworm egg is placed in freezer and refrigerates, and is released to regulation refrigeration phase, that is, outbound pickling stagnant
It educates, the silkworm egg after pickling hastens the hatching of silkworms after natural temperature dries, hatches and raises to cocooing, and the silkworm heat shock of the present invention solves immediately
Except the method for diapause and refrigeration delay hatching, first the method for heat shock is used to release the Diapause of silkworm seed immediately, then will released stagnant
The silkworm egg educated is handled by the different deepfreeze time to postpone its hatching in batches, has reached the hatching of gradation in batches
Effect fully meets the purpose of garden displaying, and tourist is made during displaying at any time can see from -1 age -2 of silkworm seed-newly-hatched silkworm
The being in -4 age of -3 age of age in -5 age-each period of cocooing brings good effect for garden.The present invention passes through simple silkworm egg
Management produces it and manages the planned needs for needing simple artificial adjusting just can meet garden displaying, saves people
Power, material resources.
Finally it should be noted that：Above-described embodiments are merely to illustrate the technical scheme, rather than to it
Limitation；Although the present invention is described in detail referring to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：
It can still modify to the technical solution recorded in previous embodiment, or to which part or all technical features into
Row equivalent replacement；And these modifications or substitutions, it does not separate the essence of the corresponding technical solution various embodiments of the present invention technical side
The range of case.
Claims (10)
1. a kind of instant termination of diapause of silkworm heat shock and the method for refrigeration delay hatching, it is characterised in that：It includes the following steps：
S1, the processed silkworm seed of pickling is urged in the climate box environment that temperature is 24~25.5 DEG C, dry and wet difference is 1.5~2 DEG C
It is green to hatching and raise to cocooing；
S2, the female male silk moth collected at a temperature of 25 DEG C of protection while changing moth, mate 4-5h under conditions of 25 DEG C, room light
Separation of copulating moth afterwards, and female moth is put in silkworm and is connected on paper, it lays eggs, lays eggs 6~8 hours in dark environment；
S3, after oviposition, be placed with silkworm seed silkworm connect paper protect in 25 DEG C of insulating box, after preset time, by silkworm connect paper use
The hot water Heat thermostability 5s of preset temperature, point three parts of processing after drying；
The silkworm seed that S4, first part of instant Heat thermostability are crossed, it is 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet to be put in temperature
After being hastened the hatching of silkworms, raising of collecting ants；The silkworm seed that second part of instant Heat thermostability is crossed, it is 24~25.5 DEG C, dry and wet poor 1.5 to be put in temperature
~2 DEG C of climate box hastens the hatching of silkworms, and after silkworm seed point blueness, is placed in middle database after 2h, wraps silkworm seed with film, be put in refrigeration
In library, took out the silkworm seed of predetermined amount in batches every 3 days and be put under 25 DEG C of environment and receive after the silkworm seed of taking-up is placed in middle database 2h
Ant is raised；After the silkworm seed that the instant Heat thermostability of third part is crossed is placed in middle database 6h, refrigerate in freezer, with thin in freezer
Film wraps silkworm seed, after 30 days, takes out the silkworm seed of predetermined amount in batches every 5 days, and the silkworm seed of taking-up is first placed in middle database for 24 hours,
Be put in again temperature be 24~25.5 DEG C, poor 1.5~2 DEG C of the climate box of dry and wet is hastened the hatching of silkworms and raising of collecting ants.
2. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
In step S4,3~20 days total holding times after the silkworm seed point blueness after second part of instant Heat thermostability in freezer.
3. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
In step S4,30~50 days storage total times in freezer of silkworm seed after the instant Heat thermostability of third part.
4. the instant termination of diapause of silkworm heat shock according to claim 2 or 3 and the method for refrigeration delay hatching, feature exist
In：In step s 4, the silkworm seed that second part of instant Heat thermostability is crossed took out once every 3 days from freezer, is divided into 6 times and takes
Go out, the silkworm seed taken out every time is the 1/6 of the silkworm seed total amount that second part of instant Heat thermostability is crossed；The instant Heat thermostability of third part is crossed
Silkworm seed took out once from freezer every 5 days, was divided into 4 taking-ups, and the silkworm seed taken out every time is the instant Heat thermostability of third part
The 1/4 of the silkworm seed total amount crossed.
5. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
The indoor luminous intensity is 50~300Lux.
6. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
The preset time is 12-24h, and the preset temperature is 53.1 ± 0.5 DEG C.
7. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
The temperature of the middle database is 15-16 DEG C.
8. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, it is characterised in that：
The temperature of the freezer is 5 ± 0.5 DEG C.
9. the instant termination of diapause of silkworm heat shock according to claim 5 and the method for refrigeration delay hatching, it is characterised in that：
The freezer is refrigerator.
10. the instant termination of diapause of silkworm heat shock according to claim 1 and the method for refrigeration delay hatching, feature exist
In：The film is polyethylene film.
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