CN102754624A - Method for delaying hatching of F1 hybrids of silkworm eggs in hatching - Google Patents
Method for delaying hatching of F1 hybrids of silkworm eggs in hatching Download PDFInfo
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Abstract
The invention discloses a method for delaying the hatching of F1 hybrids of incubated silkworm eggs in hatching. The F1 hybrids of silkworm eggs in hatching are subjected to three-section type low-temperature preservation, and graine embryos of the F1 hybrids of silkworm eggs of mulberry silkworms are grown through the hatching processing to a body pigmentation stage, and then are continuously preserved at the certain environment temperature; 10 silkworm eggs boxes are bundled by a plastic rope, and put in a double-layered black cloth bag wrung after being soaked in water, and the silkworm eggs in the 10 silkworm eggs boxes are uniformly shaken and horizontally placed; and the black cloth bag wrapping the silkworm eggs boxes is put in a certain temperature environment for preservation, and then is transferred in a refrigerator for refrigeration. Under the condition of not influencing the hatching rate and the growth of the F1 hybrids of hatched silkworm eggs, the gathering data of newly hatched silkworms is delayed by 1 to 25 days so as to be favorable for adjusting the production data of silkworm cocoons, and to reduce the influence of the natural disaster and the temperature anomaly on the growth of mulberry leaves and the influence of the silkworm poisoning and the silkworm disease and other disasters on the growth of the silkworm cocoons, and the method disclosed by the invention is a safe emergency disposal technology for sericulture, and has the hatching rate of more than 95%.
Description
Technical field
The invention belongs to the store method of silkworm silkworm seed, specifically be a kind of be suitable for hastening the hatching of silkworms in the first cross silkworm egg postpone the method for hatching.
Background technology
Silk is the Ancient Chinese greatness invention in generation, and becomes the cultural Symbol that China has world's influence.Planting mulberry and breeding silkworm have the history more than 5000 in China, still are to use new fresh mulberry leaf to breed silkworms at present, and this is natural, a precious important symbol of silk product.Based on this technical characterictic, in the now domestic and international silk cocoon large-scale production process, in order to make full use of mulberry leaf; Also in order effectively to prevent flacherie; Sericulture production in various climatic regions all relies on the tight plan silkworm egg brooding time of a cover, and its main points are to make the growth of growth and mulberry tree of silkworm synchronous; Be unlikely to because of sericulture causes very much afoliate morning, the reason sericulture is not too late yet causes mulberry leaf to wear out and sericulture later stage and other crops production generation recruitment and control of insect medication contradiction.Yet along with the aggravation of Global climate change, anomalous weather occurs frequent in recent years; Often because of spring low temperature or high temperature, Changes in weather such as emergent late frost and freeze injury cause in the silkworm egg hatching process, occurring the ANOMALOUS VARIATIONS that mulberry leaf are grown; Or even mulberry leaf growths destroyed, and has to change the time started of breeding silkworms, if can not in time adjust the time started of breeding silkworms; Then cause the waste of serious afoliate or silkworm egg, influence the sericulture income.
At present, cold storage of silkworm egg suppresses technology, is after utilizing the silkworm seed termination of diapause, and body early embryo (before third embryo of silkworms) has stronger tolerance to low temperature, regulates the production time.But silkworm egg is in case come out from freezer; After (be the word of sericulture specialty hasten the hatching of silkworms processing) handled in the hatching that begins to heat; Because developmental embryo is poor to the resistance of low temperature environment,, needs to postpone and collect ants the time even run into anomalous weather; Existing technical measures also can only be regulated 3-5 days time, and cold preservation time is long to be occurred dead germ easily and hatch bad.Go back more effective, the safer method of neither one in the silk cocoon production and suppress the silkworm seed embryonic development, postpone brooding time more than 10 days, this has greatly influenced the safety of breeding silkworms and producing.
On the other hand, between the silkworm feeding period, the large and small silkworm death by accident because of pesticide poisoning, flacherie or other reasons all can take place in various places, and do not provide as there being the silkworm egg that hastened the hatching of silkworms timely this moment, with causing the silkworm raiser who goes wrong to lose receipts.Simultaneously, because of can't regulating brooding time, raise the rich and influential family and can not rationally arrange to raise quantity according to the actual conditions of self, reach should make full use of favourable season, mulberry leaf are bred silkworms more, avoid the purpose of labour, apparatus contradiction again.Some areas will be carried out the adjustment of silkworm rearing season layout as increased by two spring raising etc., and are normal because of not providing suitable silkworm egg supply to implement.
For this reason; Invent a kind of do not influence the silkworm incubation rate with grow; The flexible middle egg-incubation time method of hastening the hatching of silkworms; For effective reduction natural calamity and thermic anomaly the influence of mulberry leaf growth and silkworm are poisoned and the influence of disasters such as flacherie to silk cocoon production; The balance, raising sericulture productivity effect and the sericulture safety that improve sericulture quantity and mulberry leaf output have important value, also for availability, the flexible silkworm rearing season layout that improves the first cross silkworm egg effective way are provided simultaneously.
Summary of the invention
Main task of the present invention is to provide the method for first cross silkworm egg delay hatching in a kind of the hastening the hatching of silkworms; It specifically is a kind of first cross silkworm egg to having hastened the hatching of silkworms; In low temperature preservation process, through adopting three sections different temperatures protections, the control silkworm seed is grown; Prolong the silkworm egg holding time, the technology of flexible egg-incubation time.
In order to solve above technical problem; The first cross silkworm egg postpones the method for hatching in a kind of the hastening the hatching of silkworms of the present invention; Its innovative point is following: the first cross silkworm egg carries out the three-sectional low temperature protection in will hastening the hatching of silkworms, and step is following: the first step: the first cross silkworm egg in hastening the hatching of silkworms, and the silkworm seed embryonic development is behind body pigmentation stage; With 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;
Second step: with the above-mentioned first cross silkworm egg that has placed in the silkworm seed box, per 10 silkworm seed boxes are a unit, and external application ash newspaper is wrapped the well-balanced tiling of silkworm egg in the silkworm seed box of each unit; Per two unit are a unit, put into the double-deck changing bag of wringing out after the immersion, and changing bag put into cover that the sheet lead case closely covers, the dark preservation, put into 2 of changing bags in each case at most;
The 3rd step: the sheet lead case is put into 13-15 ℃ of environment insulation 3-4 hour;
The 4th step: above-mentioned sheet lead case changed in 0.0 ℃ ± 0.5 ℃ the freezer; Dark surrounds protection 1---5 days, storehouse temperature to 2.5 ± 0.5 ℃ was regulated in the back, continues dark surrounds protection 6---after 15 days; Again temperature is transferred to 5 ℃ ± 0.5 ℃, dark surrounds protection 16---25 days;
The 5th step: with the first cross silkworm egg in the step 4; Take out in the section at any time; And through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handled 1-2 days, and back sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further, in said the 5th step, the 1-5 of preservation is in day; At any time in the sheet lead case, take out; Through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handled 2 days, and back sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further, in said the 5th step, the 6-15 of preservation is in day; At any time in the sheet lead case, take out; Through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handled 1 day, and back sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further, in said the 5th step, at the 16-25 that preserves in day; The first generation cross-fertilize seed of taking out in the freezer at dusk, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag; Reply 25 ℃; The dark surrounds protection of humidity 80-90%, sensitization the next morning (illumination is higher than 30LUX) impels egg-incubation.
The invention has the advantages that: 1, use the inventive method can be under the situation that does not influence the practical incubation rate of first cross silkworm egg silkworm and grow; Postponement was collected ants date 1-25 days; Help regulating the silk cocoon date of manufacture; Reducing natural calamity and thermic anomaly to the influence that the influence of mulberry leaf growth and silkworm poison and silk cocoon produced with disasters such as flacherie, improve the balance of sericulture quantity and mulberry leaf output, is that the emergency disposal of a safety of sericulture production is technological.
2, its innovative point is to have changed in the industry and generally believes that the first cross silkworm egg after beginning to hasten the hatching of silkworms can only be postponed 3-5 days viewpoint of silkworm egg brooding time through supplementary means.The silkworm seed embryonic development is to body pigmentation stage in hastening the hatching of silkworms; Through adopting 0.0 ± 0.5 ℃ of deepfreeze; And, improve the three-sectional low temperature guard method of 2.5 ℃ of protection temperature respectively at the 6th day, the 16th day that refrigerates, prolong effective cold preservation time of first cross silkworm egg; The collect ants arrangement of time of control silk cocoon production realizes silkworm rearing season layout and the flexible of silk cocoon date of manufacture.
3, help the regulation and control sericulture and collect ants the time, regulate kind of a leaf balance; Help the safety that silk cocoon is produced; Help reducing the silkworm raiser because of the calamity loss; Help making full use of of first cross silkworm egg; The emergency disposal that helps the silkworm and mulberry accident.
4, the incubation rate of above-mentioned silkworm first cross silkworm egg reaches more than 95%.
Embodiment
Embodiment 1
Perfectly sound village, evergreen town, Rugao City, Jiangsu in 2010, north latitude 32.23 east longitudes 120.33 have a low-lying mulberry field, 56 mu of areas; Because of 4.29 days late frost influence, the mulberry tree young sprout suffered freeze injury, the growing point blackout, and blade is dried-up; Growing of mulberry is stagnated, and ordered 65 silkworm eggs of 38 family silkworm raisers this moment outbound begin to hasten the hatching of silkworms, in order to reduce silkworm raiser's loss; Prevent to cause the silkworm egg waste, we adopt following method refrigeration silkworm egg, prolong and hasten the hatching of silkworms the time:
1. the first cross silkworm egg (Su Zhen * spring scenery) in will hastening the hatching of silkworms, silkworm seed embryonic development are behind body pigmentation stage, and with 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;
Above-mentioned first cross silkworm egg is good with per 10 the silkworm seed box bundles ofs of plastic ties 2.; Silkworm egg shakes up tiling in the silkworm seed box; External application ash newspaper is wrapped; Put into the double-deck changing bag of wringing out after the immersion, keep flat silkworm seed box 2 in each bag and prick, and the changing bag that will wrap up silkworm seed box put into the general 40cm * 50cm of industry, high 40cm cover the sheet lead case;
3. the sheet lead case is put into 13-15 ℃ of environment protection 3-4 hour;
4. above-mentioned sheet lead case is changed in 0.0 ℃ ± 0.5 ℃ the freezer, dark surrounds protection 5 days, storehouse temperature to 2.5 ± 0.5 ℃ is regulated in the back, continues dark surrounds protection 5 days;
5. the first cross silkworm egg of in freezer, preserving; Specific as follows: in the sheet lead case, take out silkworm egg, through 13-15 ℃ medium temperature 3-4 hour, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds; Remove changing bag, spread out kind, mend to hasten the hatching of silkworms and handled 1 day, back sensitization (illumination is higher than 30LUX) impels egg-incubation;
6. adopt above method protection, the practical incubation rate of first cross silkworm egg is more than 95%;
Embodiment 2
Between autumn silkworm rearing season, Yang Zhuan village, Dong Chen town, Rugao, Jiangsu, minister village cause 545 silkworm eggs of two villages, 286 family silkworm raisers silkworm in 3 age to be poisoned to death because of paddy rice large tracts of land misuse chlopyrifos in 2009.Adopt the method for embodiment 1 in time low-temperature protection to be suppressed 545 first cross silkworm eggs of 15 days this moment, reissue to the silkworm raiser and raise, and avoided more than 80 ten thousand yuan of silkworm raisers' loss.
Embodiment 3
In recent years; Be the influence that the minimizing various factors is produced silkworm and mulberry, each silkworm rearing season, Rugao City, Jiangsu Province is all when the silkworm egg outbound; Go out to account for silkworm egg in advance and provide the first cross silkworm egg of total amount 10%; Adopt the method to make low-temperature protection in the later stage of hastening the hatching of silkworms, postpone the silkworm egg hatching, the sericulture loss of having avoided a variety of causes to cause effectively.In the time of at most, a silkworm rearing season, 1508 of the silkworm eggs of this method protection of granting employing altogether.
Silkworm egg in the above table is taken out the processing of hastening the hatching of silkworms that the back is done from refrigerated room,, have nothing in common with each other according to the different time section, specific as follows:
The 1-5 that preserves takes out in the sheet lead case in day at any time, through 13-15 ℃ medium temperature 3-4 hour; The answer later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, and humidity 80-90% dark surrounds removes changing bag; Spread out kind, mend to hasten the hatching of silkworms and handled 2 days, back sensitization (illumination is higher than 30LUX) impels egg-incubation.
The 6-15 that preserves takes out in the sheet lead case in day at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms to handle after 1 day and collect ants.
The 16-25 that preserves is in day, and the first generation cross-fertilize seed of taking out in the freezer is at dusk removed changing bag; Through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, reply 25 ℃; The dark surrounds of humidity 80-90% spreads out kind, and sensitization the next morning (illumination is higher than 30LUX) impels egg-incubation.
Embodiment 4
Xue An town, Rugao, Jiangsu snow bank 30 groups of Xu Ruifeng of neighbourhood committee have 14 mu of mulberry fields, husband and wife's sericultures of being in, and owing to the restriction of the labour of family, apparatus and cocoonery area etc., each issue silkworm can only be raised 12 silkworm eggs at most.Generally speaking, in order to make full use of mulberry leaf, silkworm in spring is raised 16-18 and opens, and raises 14 the mid-autumn, raises 12 late fall, the annual sericulture about 42.Because the disposable number of animals raised is big, husband and wife are too busy to do it, and silkworm need be asked 2 workman's mulberry leaf harvest leaves 5 length of times, adds the deficiency of sericulture house, apparatus; Open kind of an area during 5 ages and put deficiency, cause and nibble the mulberry deficiency, the silkworm body is little; Grid a small bundle of straw, etc. for silkworms to spin cocoons on is not enough when being placed on small straw bundles to spin cocoons again, uses careless imperial a small bundle of straw, etc. for silkworms to spin cocoons on, and cocoon quality is reduced; Or the like, the silk cocoon per unit area yield of selling is low, and unit price is low again.Begin from spring silkworm rearing season in 2010,, collect ants in batches through adopting method of the present invention; Postpone than first for second batch and collecting ants in 16 days, the silkworm of both having staggered is raised and big busy period of the harvesting of cocoon of being placed on small straw bundles to spin cocoons 5 ages, stops the disinfecting time of big silkworm room, tool; Prolong mulberry leaf vegetative period again, improve mulberry leaf output, improve sericulture quantity; Increased cocoon yield and benefit, reduced and asked worker's cost, achieved many things at one stroke.Details are seen table:
Visible by last table, many to sericulture amount casual household can pass through this method, rationally adjusts feeding time, and the breed of staggering reduces labour intensity.Directly reduce aquaculture cost.
Claims (4)
- One kind hasten the hatching of silkworms in the first cross silkworm egg postpone the method for hatching, it is characterized in that: the first cross silkworm egg carries out the three-sectional low temperature protection in will hastening the hatching of silkworms, and step is following:The first step: the first cross silkworm egg in hastening the hatching of silkworms, silkworm seed embryonic development are behind body pigmentation stage, and with 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;Second step: with the above-mentioned first cross silkworm egg that places in the silkworm seed box, per 10 silkworm seed boxes are a unit, and external application ash newspaper is wrapped the well-balanced tiling of silkworm egg in the silkworm seed box of each unit; Per two unit are a unit, put into the double-deck changing bag of wringing out after the immersion, and changing bag put into cover that the sheet lead case closely covers, the dark preservation, put into 2 of changing bags in each case at most;The 3rd step: the sheet lead case is put into 13-15 ℃ of environment insulation 3-4 hour;The 4th step: above-mentioned sheet lead case changed in 0.0 ℃ ± 0.5 ℃ the freezer; Dark surrounds protection 1---5 days, storehouse temperature to 2.5 ± 0.5 ℃ was regulated in the back, continues dark surrounds protection 6---after 15 days; Again temperature is transferred to 5 ℃ ± 0.5 ℃, dark surrounds protection 16---25 days;The 5th step: with the first cross silkworm egg in the step 4; Take out in the section at any time; Through 13-15 ℃ medium temperature 3-4 hour, changing bag is removed in the back, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handled 1-2 days, and back sensitization (illumination is higher than 30LUX) impels egg-incubation.
- 2. the first cross silkworm egg postpones the method for hatching in a kind of the hastening the hatching of silkworms according to claim 1, it is characterized in that: in said the 5th step, the 1-5 that preserves at silkworm egg is in day; At any time in the sheet lead case, take out; Through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handled 2 days, and back sensitization (illumination is higher than 30LUX) impels egg-incubation.
- 3. the first cross silkworm egg postpones the method for hatching in a kind of the hastening the hatching of silkworms according to claim 1, it is characterized in that: in said the 5th step, the 6-15 that preserves at silkworm egg is in day; At any time in the sheet lead case, take out; Through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature; Humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and handle after 1 day, and sensitization (illumination is higher than 30LUX) impels egg-incubation.
- 4. the first cross silkworm egg postpones the method for hatching in a kind of the hastening the hatching of silkworms according to claim 1, it is characterized in that: in said the 5th step, at the 16-25 that preserves in day; At dusk in freezer, take out first generation cross-fertilize seed, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag; Reply 25 ℃; The dark surrounds protection of humidity 80-90%, sensitization the next morning (illumination is higher than 30LUX) impels egg-incubation.
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Cited By (4)
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CN104509496A (en) * | 2014-11-28 | 2015-04-15 | 太和县金秋农民养殖专业合作社 | Silkworm breeding method |
CN105941347A (en) * | 2016-05-16 | 2016-09-21 | 王月兰 | Method using square cocoon frame to grow silkworm and cocoon |
CN110754442A (en) * | 2019-08-06 | 2020-02-07 | 德清县莫干天竺蚕种有限责任公司 | Silkworm embryo low-temperature protection and seed-delay technology |
CN112106738A (en) * | 2020-09-11 | 2020-12-22 | 河南省蚕业科学研究院 | Method for nursing one or two-release autumn tussah to relieve diapause |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104509496A (en) * | 2014-11-28 | 2015-04-15 | 太和县金秋农民养殖专业合作社 | Silkworm breeding method |
CN105941347A (en) * | 2016-05-16 | 2016-09-21 | 王月兰 | Method using square cocoon frame to grow silkworm and cocoon |
CN105941347B (en) * | 2016-05-16 | 2018-12-04 | 南京电狐网络科技有限公司 | A method of it is bred silkworms and is cocoond using a rectangular small bundle of straw |
CN110754442A (en) * | 2019-08-06 | 2020-02-07 | 德清县莫干天竺蚕种有限责任公司 | Silkworm embryo low-temperature protection and seed-delay technology |
CN112106738A (en) * | 2020-09-11 | 2020-12-22 | 河南省蚕业科学研究院 | Method for nursing one or two-release autumn tussah to relieve diapause |
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