CN112042766A - 一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 - Google Patents
一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 Download PDFInfo
- Publication number
- CN112042766A CN112042766A CN202010977072.7A CN202010977072A CN112042766A CN 112042766 A CN112042766 A CN 112042766A CN 202010977072 A CN202010977072 A CN 202010977072A CN 112042766 A CN112042766 A CN 112042766A
- Authority
- CN
- China
- Prior art keywords
- parts
- powder
- yak milk
- milk powder
- chitosan oligosaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000843 powder Substances 0.000 title claims abstract description 68
- 235000020255 yak milk Nutrition 0.000 title claims abstract description 56
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 39
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 39
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 37
- 239000006041 probiotic Substances 0.000 title claims abstract description 31
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 31
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 30
- 235000013350 formula milk Nutrition 0.000 title claims abstract description 21
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 18
- 102000014171 Milk Proteins Human genes 0.000 claims abstract description 16
- 108010011756 Milk Proteins Proteins 0.000 claims abstract description 16
- 239000000047 product Substances 0.000 claims abstract description 16
- 239000005862 Whey Substances 0.000 claims abstract description 12
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 12
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 12
- 235000008939 whole milk Nutrition 0.000 claims abstract description 12
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims abstract description 10
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 10
- 229940009289 bifidobacterium lactis Drugs 0.000 claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 230000004151 fermentation Effects 0.000 claims abstract description 10
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 10
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 claims abstract description 9
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 9
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 claims abstract description 9
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 9
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 9
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 9
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 9
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 9
- 239000011718 vitamin C Substances 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 7
- 238000007580 dry-mixing Methods 0.000 claims abstract description 5
- 230000001580 bacterial effect Effects 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000036039 immunity Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 239000008280 blood Substances 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 3
- 229920001542 oligosaccharide Polymers 0.000 abstract description 3
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 230000006870 function Effects 0.000 abstract description 2
- 210000002216 heart Anatomy 0.000 abstract description 2
- 230000004217 heart function Effects 0.000 abstract description 2
- 210000004185 liver Anatomy 0.000 abstract description 2
- 230000004199 lung function Effects 0.000 abstract description 2
- 230000035790 physiological processes and functions Effects 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 12
- 230000000968 intestinal effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000009182 swimming Effects 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 229960004397 cyclophosphamide Drugs 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 208000008425 Protein deficiency Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010049040 Weight fluctuation Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000037080 exercise endurance Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000020604 functional milk Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/06—Mixtures of whey with milk products or milk components
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2210/00—Physical treatment of dairy products
- A23C2210/20—Treatment using membranes, including sterile filtration
- A23C2210/202—Treatment of milk with a membrane before or after fermentation of the milk, e.g. UF of diafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2210/00—Physical treatment of dairy products
- A23C2210/20—Treatment using membranes, including sterile filtration
- A23C2210/206—Membrane filtration of a permeate obtained by ultrafiltration, nanofiltration or microfiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
一种壳寡糖益生菌牦牛乳多肽配方奶粉,由如下重量份的原料制成:全脂乳粉50‑65份、脱盐乳清粉10‑30份、全脂牦牛乳粉5‑15份、牦牛乳蛋白多肽粉5‑15份、壳寡糖5‑15份、维生素C 0.005‑0.01份、磷脂酰丝氨酸0.90‑0.95份、乳双歧杆菌(HN‑019)0.01‑0.02份、嗜酸乳杆菌(NCMF)0.01‑0.02份、保加利亚乳杆菌0.01‑0.02份、嗜热链球菌0.01‑0.02份。通过发酵、离心、超滤、干混等工艺制成成品。本发明组分搭配合理、营养全面,在为人体补充优质蛋白质的同时,其中含有的功能性低聚糖壳寡糖,具有提高身体免疫、降血脂、调节血糖、改善肝脏和心肺功能及其它多种生理功能。
Description
技术领域
本发明属于乳制品领域,具体涉及一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法。
背景技术
研究表明,益生菌对机体的健康生理具有多种作用,能够抑制肠内有害菌促进肠内有益菌的增殖, 或者减少病原体繁殖生长的营养物质, 在肠道中形成“竞争排斥”的生态平衡环境,同时,产生发酵非消化淀粉的酶, 而这些非消化淀粉是肠道微生物群落的主要能源物质,另外机体可以利用肠道益生菌产生的短链脂肪酸防止胆汁对 DNA的损害,清除自由基,并调节自身细胞活性 。
随着社会的发展进步,工作节奏的加快,越来越多的人忽视自身营养问题,再加上膳食的不均衡,结构单一,导致很多潜在疾病的爆发,如:肠道菌群失衡造成的腹泻、便秘、消化不良,蛋白质的缺乏造成的机体代谢失常、免疫力低下等。壳寡糖作为一种功能性低聚糖,经国内外大量研究验证,能够活化肠内益生菌,抑制肠道中致病菌的生长,促进人体对矿物质的吸收,提高机体免疫力,因此,开发壳寡糖功能性系列牦牛乳制品必将有广阔的市场前景。
发明内容
本发明的目的在于提供一种壳寡糖益生菌牦牛乳多肽配方奶粉,以解决现有功能性乳制品品种单一的问题。
本发明另一个目的在于提供一种壳寡糖益生菌牦牛乳多肽配方奶粉的制备方法,具有成本低,能有效解决酶解过程中产生苦味肽的问题。
本发明所采用的技术方案为:
一种壳寡糖益生菌牦牛乳多肽配方奶粉,该配方奶粉由如下重量份的原料制成:全脂乳粉50-65份、脱盐乳清粉10-30份、全脂牦牛乳粉5-15份、牦牛乳蛋白多肽粉5-15份、壳寡糖5-15份、维生素C 0.005-0.01份、磷脂酰丝氨酸0.90-0.95份、乳双歧杆菌,HN-019,0.01-0.02份、嗜酸乳杆菌,NCMF,0.01-0.02份、保加利亚乳杆菌0.01-0.02份、嗜热链球菌0.01-0.02份。
一种壳寡糖益生菌牦牛乳多肽配方奶粉的制备方法,步骤如下:
A、发酵:将枯草芽孢杆菌活化,振荡培养6-12h,得到菌液,再以新鲜牦牛乳为底物混合菌液发酵,结束后经高压灭菌得到混合产物;
B、离心:将灭菌后的混合产物在6 000 r/min离心机中离心10-15 min,得到上清液;
C、超滤:将得到的上清液经 0.45 μm 滤膜过滤,去除菌体及残渣;采用截留分子量为10 kDa、的超滤离心管对膜过滤液进行分离,再经冷冻干燥得到牦牛乳蛋白多肽产品。
干混 :将冷冻干燥好的牦牛乳蛋白多肽和全脂乳粉、脱盐乳清粉、全脂牦牛乳粉、牦牛乳蛋白多肽粉、壳寡糖、维生素C、磷脂酰丝氨酸在紫外线强度≥70μw/cm2条件下,杀菌30-50s,最后加入益生菌干混120-900s,既得成品。
本发明的优点具体如下:
(1)本发明添加的壳寡糖是一种功能性低聚糖,能够补充足够的膳食纤维以及充足菌群生长因子,具有提高身体免疫、降血脂、调节血糖、改善肝脏和心肺功能、抗疲劳、美容及其它多种生理功能外,其与保加利亚乳杆菌、乳双歧杆菌(HN-019)等配伍,能够增殖有益菌,增强有益菌的数量和活性;与牦牛乳蛋白多肽、全脂乳粉、脱盐乳清粉、全脂牦牛乳粉结合,能促进人体对乳品中钙镁等矿物质的吸收。
(2)本发明添加的全脂牦牛乳粉,具有多种活性物质,能够在为人体补充充足蛋白质的同时能提高人体自身免疫力。
(3)本发明添加的益生菌与全脂乳粉、脱盐乳清粉以及全脂牦牛乳粉相互配伍,能有效地提高益生菌的存活率,并为益生菌的生长提供必需的氨基酸。
(4)本发明添加的益生菌与牦牛乳多肽相结合,增强产品的免疫力功效,且多肽类物质提供的矿物质与维生素能有效的促进肠道益生菌的生长,二者协同作用,相互促进。
(5)本发明益生菌有乳双歧杆菌(HN-019)、嗜酸乳杆菌(NCMF)、保加利亚乳杆菌及嗜热链球菌,四种菌种结合,互利共生,能够维持肠道菌群平衡,促进人体矿物质与维生素等营养素的吸收,并能有效地防止与缓解乳糖不耐症。
(6) 现有技术中制取牦牛乳多肽一般采用酶解法,而本发明采用发酵法制备牦牛乳多肽,不仅制造成本降低,而且有效解决酶解过程中产生苦味肽的问题,提高食物的感官品质和营养价值。
(7)本发明中全脂乳粉、脱盐乳清粉与全脂牦牛乳粉的相互搭配,能为人体提供丰富的蛋白质以及充足的维生素与矿物质,其营养全面,更有益于健康。
具体实施方式
下面结合具体实施例对本发明进一步说明。
实施例1,一种壳寡糖益生菌牦牛乳多肽配方奶粉,该配方奶粉由如下重量份的原料制成:全脂乳粉65份、脱盐乳清粉10份、全脂牦牛乳粉5份、牦牛乳蛋白多肽粉8份、壳寡糖5份、磷脂酰丝氨酸0.90份、维生素C 0.01份、乳双歧杆菌(HN-019)0.02份、嗜酸乳杆菌(NCMF)0.02份、保加利亚乳杆菌0.01份、嗜热链球菌0.01份。
实施例2,一种壳寡糖益生菌牦牛乳多肽配方奶粉,该配方奶粉由如下重量份的原料制成:全脂乳粉50份、脱盐乳清粉25份、全脂牦牛乳粉10份、牦牛乳蛋白多肽粉5份、壳寡糖9份、维生素C 0.005份、磷脂酰丝氨酸0.94份、乳双歧杆菌(HN-019)0.01份、嗜酸乳杆菌(NCMF)0.01份、保加利亚乳杆菌0.02份、嗜热链球菌0.02份。
实施例3,一种壳寡糖益生菌牦牛乳多肽配方奶粉,该配方奶粉由如下重量份的原料制成:全脂乳粉60份、脱盐乳清粉30份、全脂牦牛乳粉15份、牦牛乳蛋白多肽粉15份、壳寡糖15份、维生素C 0.008份、磷脂酰丝氨酸0.95份、乳双歧杆菌(HN-019)0.015份、嗜酸乳杆菌(NCMF)0.015份、保加利亚乳杆菌0.015份、嗜热链球菌0.015份。
实施例1-3所述的壳寡糖益生菌牦牛乳多肽配方奶粉的制备工艺,包括以下步骤:
A、发酵:将枯草芽孢杆菌活化,振荡培养6-12h,得到菌液,再以新鲜牦牛乳为底物混合菌液发酵,结束后经高压灭菌得到混合产物。所得菌液是1×109 cfu/mL,底物质量浓度为80 份/mL ,新鲜牦牛乳接种体积分数为 2.7%菌液,30-36 ℃,135 r/min 振荡培养发酵60-70 h,发酵结束后120 ℃高压灭5-10min 。
B、离心:将灭菌后的混合产物在6 000 r/min离心机中离心10-15 min,得到上清液。
C、超滤:将得到的上清液经 0.45 μm 滤膜过滤,去除菌体及残渣;采用截留分子量为 10 kDa、的超滤离心管对膜过滤液进行分离(4 000 r/min,10 min),再经冷冻干燥得到牦牛乳蛋白多肽产品。
D、干混 :将冷冻干燥好的牦牛乳蛋白多肽和除去益生菌的各原料在紫外线强度≥70μw/cm2条件下,杀菌30-50s,最后加入益生菌干混120-900s,既得成品。
本发明的动物性试验
1、抗疲劳试验:20只健康雄性小鼠,随机分为两组,每组10只,空白对照组灌胃5ml生理盐水,另一组每天灌胃5ml实施例1配方奶粉奶液1次,连续灌胃30d,灌胃期间各组小鼠喂食与饮水量一致。最后一次灌胃结束1h后,各组随机选取5只小鼠,开展负重游泳试验,记录小鼠自入水开始游泳至沉没超过10s的时间,即为动物负重游泳时间。
运动耐力可反映机体抗运动性疲劳的程度,而负重游泳是常见评估身体抗疲劳能力的运动实验模型,因此考察配方奶粉对小鼠的负重游泳时间长短的影响,结果如下表。
配方奶粉对小鼠运动时间的影响
从上表可知,与空白对照组相比,阳性对照组小鼠的力竭游泳时间延长率为14.7%,由此可知壳寡糖益生菌牦牛乳多肽配方奶粉有助于延长运动时间,具有抗疲劳的功效。
2、本发明四种菌种组合组比其他单一菌种效果试验:选取60只雄性小白鼠,先进行适应性饲养一周,然后随机分成六组,分别为乳双歧杆菌(HN-019)组、嗜酸乳杆菌(NCMF)组、保加利亚乳杆菌组、嗜热链球菌组、四种菌种组合组及对照组,对照组喂养生理盐水,实验组喂养(1.5x109CFU/ml)菌悬液0.2ml,连续三天注射环磷酰胺(80m份/k份),干预四周,每周称两次体重,求取平均值。小白鼠体重及偏差结果如下:
第一周给小白鼠注射环磷酰胺后免疫力降低,实验组小白鼠体重均有所下降,第二周实验组小白鼠体重回升,通过四周的观察和称重,记录数据,并进行分析,结果表明,四种菌群组合组标准偏差小于其他四组实验组,体重波动较小。可以说明,四种菌种组合组比其他单一菌种实验组更能有效的维持小白鼠体重,并且能够提高小白鼠免疫力。
Claims (5)
1.一种壳寡糖益生菌牦牛乳多肽配方奶粉,其特征在于,该配方奶粉由如下重量份的原料制成:全脂乳粉50-65份、脱盐乳清粉10-30份、全脂牦牛乳粉5-15份、牦牛乳蛋白多肽粉5-15份、壳寡糖5-15份、维生素C 0.005-0.01份、磷脂酰丝氨酸0.90-0.95份、乳双歧杆菌,HN-019,0.01-0.02份、嗜酸乳杆菌,NCMF,0.01-0.02份、保加利亚乳杆菌0.01-0.02份、嗜热链球菌0.01-0.02份。
2.根据权利要求1所述的一种壳寡糖益生菌牦牛乳多肽配方奶粉,其特征在于,该配方奶粉由如下重量份的原料制成:全脂乳粉65份、脱盐乳清粉10份、全脂牦牛乳粉5份、牦牛乳蛋白多肽粉8份、壳寡糖5份、磷脂酰丝氨酸0.90份、维生素C 0.01份、乳双歧杆菌,HN-019,0.02份、嗜酸乳杆菌,NCMF,0.02份、保加利亚乳杆菌0.01份、嗜热链球菌0.01份。
3.根据权利要求1或2所述的一种壳寡糖益生菌牦牛乳多肽配方奶粉的制备方法,其特征如下:
发酵:将枯草芽孢杆菌活化,振荡培养6-12h,得到菌液,再以新鲜牦牛乳为底物混合菌液发酵,结束后经高压灭菌得到混合产物;
离心:将灭菌后的混合产物在6 000 r/min离心机中离心10-15 min,得到上清液;
超滤:将得到的上清液经 0.45 μm 滤膜过滤,去除菌体及残渣;采用截留分子量为 10kDa、的超滤离心管对膜过滤液进行分离,再经冷冻干燥得到牦牛乳蛋白多肽产品。
4.干混 :将冷冻干燥好的牦牛乳蛋白多肽和全脂乳粉、脱盐乳清粉、全脂牦牛乳粉、牦牛乳蛋白多肽粉、壳寡糖、维生素C、磷脂酰丝氨酸在紫外线强度≥70μw/cm2条件下,杀菌30-50s,最后加入益生菌干混120-900s,既得成品。
5.根据权利要求3所述的一种壳寡糖益生菌牦牛乳多肽配方奶粉的制备方法,其特征在于:所得菌液是1×109 cfu/mL,底物质量浓度为 80 份/mL ,新鲜牦牛乳接种体积分数为 2.7%菌液,30-36 ℃,135 r/min 振荡培养发酵 60-70 h,发酵结束后120 ℃高压灭5-10min 。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010977072.7A CN112042766A (zh) | 2020-09-16 | 2020-09-16 | 一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010977072.7A CN112042766A (zh) | 2020-09-16 | 2020-09-16 | 一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112042766A true CN112042766A (zh) | 2020-12-08 |
Family
ID=73603015
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010977072.7A Pending CN112042766A (zh) | 2020-09-16 | 2020-09-16 | 一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112042766A (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114680169A (zh) * | 2022-04-20 | 2022-07-01 | 陕西师范大学 | 含有四种高免疫活性蛋白的冷冻干燥乳粉的制备方法 |
CN114788540A (zh) * | 2022-04-20 | 2022-07-26 | 甘南倍益特生物科技有限公司 | 一种适用于高原人群的牦牛乳多肽配方奶粉及制备方法 |
CN114831183A (zh) * | 2022-05-12 | 2022-08-02 | 甘南倍益特生物科技有限公司 | 一种抗高原反应配方奶粉及其制备方法 |
CN114916590A (zh) * | 2022-05-20 | 2022-08-19 | 甘南牦牛乳研究院 | 一种牦牛乳酥油脱膻增香方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106509138A (zh) * | 2016-11-27 | 2017-03-22 | 杨凌众羊联合牧场有限公司 | 一种适用于中老年人的羊奶粉及其制备方法 |
CN107212097A (zh) * | 2017-04-01 | 2017-09-29 | 高培(广州)乳业有限公司 | 一种益于中老年人肠道健康的奶粉 |
CN109874869A (zh) * | 2019-04-11 | 2019-06-14 | 和政县华龙乳制品有限公司 | 牦牛乳酸奶粉及其制备方法 |
CN110037124A (zh) * | 2019-05-29 | 2019-07-23 | 湖北咸宁向阳湖兴兴奶业有限公司 | 一种益生菌水果牛乳粉 |
CN110037125A (zh) * | 2019-04-11 | 2019-07-23 | 甘肃华羚生物技术研究中心 | 一种儿童益智型牦牛乳多肽营养粉及其制备方法 |
CN110946182A (zh) * | 2019-12-24 | 2020-04-03 | 伊犁那拉乳业集团有限公司 | 一种可调理肠胃的乳酸菌配方奶粉制备方法及组成 |
-
2020
- 2020-09-16 CN CN202010977072.7A patent/CN112042766A/zh active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106509138A (zh) * | 2016-11-27 | 2017-03-22 | 杨凌众羊联合牧场有限公司 | 一种适用于中老年人的羊奶粉及其制备方法 |
CN107212097A (zh) * | 2017-04-01 | 2017-09-29 | 高培(广州)乳业有限公司 | 一种益于中老年人肠道健康的奶粉 |
CN109874869A (zh) * | 2019-04-11 | 2019-06-14 | 和政县华龙乳制品有限公司 | 牦牛乳酸奶粉及其制备方法 |
CN110037125A (zh) * | 2019-04-11 | 2019-07-23 | 甘肃华羚生物技术研究中心 | 一种儿童益智型牦牛乳多肽营养粉及其制备方法 |
CN110037124A (zh) * | 2019-05-29 | 2019-07-23 | 湖北咸宁向阳湖兴兴奶业有限公司 | 一种益生菌水果牛乳粉 |
CN110946182A (zh) * | 2019-12-24 | 2020-04-03 | 伊犁那拉乳业集团有限公司 | 一种可调理肠胃的乳酸菌配方奶粉制备方法及组成 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114680169A (zh) * | 2022-04-20 | 2022-07-01 | 陕西师范大学 | 含有四种高免疫活性蛋白的冷冻干燥乳粉的制备方法 |
CN114788540A (zh) * | 2022-04-20 | 2022-07-26 | 甘南倍益特生物科技有限公司 | 一种适用于高原人群的牦牛乳多肽配方奶粉及制备方法 |
CN114831183A (zh) * | 2022-05-12 | 2022-08-02 | 甘南倍益特生物科技有限公司 | 一种抗高原反应配方奶粉及其制备方法 |
CN114916590A (zh) * | 2022-05-20 | 2022-08-19 | 甘南牦牛乳研究院 | 一种牦牛乳酥油脱膻增香方法 |
CN114916590B (zh) * | 2022-05-20 | 2024-05-14 | 甘南牦牛乳研究院 | 一种牦牛乳酥油脱膻增香方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112042766A (zh) | 一种壳寡糖益生菌牦牛乳多肽配方奶粉及其制备方法 | |
Beheshtipour et al. | Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks | |
EP1478246B1 (en) | Dietetic and/or pharmaceutical compositions for human and/or animal use based on probiotic microbial preparations | |
CN101616680B (zh) | 内脏脂肪减少剂 | |
CN107259578A (zh) | 一种益生菌制品及其制备方法 | |
CN101454439B (zh) | 免疫调节活性高的乳酸菌的培养法 | |
CN103271274B (zh) | 一种营养保健粥 | |
Fadaei et al. | Influence of Spirulina platensis powder on the starter culture viability in probiotic yoghurt containing spinach during cold storage | |
US10576113B2 (en) | Method of preparing stable, water soluble probiotic compositions based on millets and similar cereals | |
Solomon et al. | Substantiation of the technology for fermented sour-milk desserts with bifidogenic properties | |
CN101240255A (zh) | 一种用于植物源蛋白的益生菌发酵剂的制备方法 | |
CN109929779A (zh) | 一种含有生物活性肽的益生菌制剂及其制备方法和应用 | |
CN109287749B (zh) | 一种富含活性植物乳杆菌的双蛋白发酵乳及其制备方法 | |
KR20060135016A (ko) | 유카 추출물, 퀼라야 추출물 및 유산균을 포함하는 조성물및 상기 조성물을 함유하는 음식물 | |
JP3648233B2 (ja) | 飼料組成物 | |
CN101744048B (zh) | 一种纳豆酸奶及其制备方法 | |
CN109601620A (zh) | 含有植物乳杆菌n3117的发酵乳及其制备方法 | |
CN102742653A (zh) | 一种健脑安神益生菌羊奶片及其制备方法 | |
CN108795823A (zh) | 一种改善女性孕产妇肠道菌群的益生菌的培养方法和应用 | |
CN111685255A (zh) | 一种增强免疫功能的益生菌固体饮料及其制备方法 | |
CN111357905A (zh) | 一种黑米谷物酵饮的制作方法 | |
CN106472670A (zh) | 一种运动营养型发酵乳及其制备方法 | |
CN102754695A (zh) | 一种牛蒡益生菌羊奶片及其制备方法 | |
CN108094531A (zh) | 一种双重益生菌发酵饮品及其制备方法 | |
CN103555801A (zh) | 乳酸菌固态发酵酶解豆粕制备多肽的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201208 |
|
RJ01 | Rejection of invention patent application after publication |