CN111821427A - Traditional Chinese medicine preparation for preventing and treating poultry colibacillosis and preparation method thereof - Google Patents
Traditional Chinese medicine preparation for preventing and treating poultry colibacillosis and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/638—Ligustrum, e.g. Chinese privet
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
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- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention aims to provide a traditional Chinese medicine preparation for preventing and treating poultry colibacillosis and a preparation method thereof, and the traditional Chinese medicine preparation is characterized in that FN (fibronectin) and fermented traditional Chinese medicines are compounded, and the addition amount of FN in the fermented traditional Chinese medicines is 0.1-2.0% by weight; the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials: 320-420 parts of red date, 170 parts of glossy privet fruit 145-; probiotic fermentation liquor: the addition amount is 3-6%. The preparation perfectly combines traditional Chinese medicines, probiotics and FN, can adjust animal intestinal microecological balance, purify intestinal environment, improve animal immunity, resist infection, stimulate mucosa cell regeneration, promote gastrointestinal mucosa repair, accelerate the recovery of animals infected with colibacillosis, and has effects of invigorating spleen and kidney, strengthening body resistance and consolidating constitution, and treating both principal and secondary aspect of disease.
Description
Technical Field
The invention belongs to the technical field of medicine preparation, and particularly relates to a traditional Chinese medicine preparation for preventing and treating poultry colibacillosis and a preparation method thereof.
Background
The pathogen of poultry colibacillosis is escherichia coli, which is a common infectious disease, poultry of various ages of days can be infected with the colibacillosis, and clinically, the colibacillosis is characterized by diarrhea, yellow or yellowish-white feces, has high death rate and great harm to poultry. The most commonly used for treating the disease are various antibacterial drugs, such as colistin sulfate, florfenicol, kanamycin sulfate, ciprofloxacin hydrochloride and the like; the long-term over-range and over-dosage use of antibiotics and other conventional medicines causes the use dosage to be greatly increased and the curative effect to be reduced, and increases the residues of the medicines in the poultry and the harm of the toxic and side effects thereof to human bodies. The traditional Chinese herbal medicine or decoction and the existing western medicine can not quickly and effectively treat the diseases of poultry, such as acute septicemia, poult navel inflammation, air sacculitis, gastritis, enteritis, peritonitis, ulcer and the like caused by gastrointestinal mucosa injury due to escherichia coli infection.
Therefore, the development of a specific drug for preventing and treating poultry colibacillosis, which has the advantages of obvious effect, stable effect, convenient use, safety, low toxicity, environmental protection, and great significance.
Disclosure of Invention
The invention aims to overcome the defects and provide the traditional Chinese medicine preparation which has reasonable formula, no medicine residue, no medicine resistance, high bioavailability and obvious effect and can effectively prevent and treat the colibacillosis of poultry. The preparation perfectly combines traditional Chinese medicines, probiotics and FN, can adjust animal intestinal microecological balance, purify intestinal environment, improve animal immunity, stimulate mucosal cell regeneration, promote gastrointestinal mucosa repair, accelerate the recovery of animals infected with colibacillosis, and has effects of invigorating spleen and kidney, strengthening body resistance, and treating both principal and secondary aspect of disease. Meanwhile, the invention also provides a corresponding preparation method which is convenient for large-scale production and is environment-friendly.
The purpose of the invention is realized by the following technical scheme: a Chinese medicinal preparation for preventing and treating poultry colibacillosis is characterized in that FN (fibronectin) lyophilized powder and fermented traditional Chinese medicine are compounded, wherein the addition amount of the FN lyophilized powder in the fermented traditional Chinese medicine is 0.1-2.0% by weight;
the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials in parts by weight: the raw material medicaments: 320-420 parts of red date, 170 parts of glossy privet fruit 145-; probiotic fermentation liquor: the addition amount is 3-6%.
The probiotic fermentation liquid is a cultured probiotic culture liquid, and the probiotic comprises a mixture of more than two of clostridium butyricum, lactobacillus acidophilus, candida utilis, lactobacillus plantarum, enterococcus faecalis, pediococcus acidilactici or bacillus subtilis.
The preparation method of the fermented traditional Chinese medicine preparation for preventing and treating poultry colibacillosis is characterized by comprising the following steps of:
(1) superfine grinding: respectively carrying out superfine grinding on various traditional Chinese medicine components in the formula and then sieving the traditional Chinese medicine components with a 500-mesh sieve;
(2) feeding and mixing: accurately weighing the required raw materials which are sieved by a 500-mesh sieve according to the specified proportion, putting the raw materials into a charging bucket, dry-mixing for 20-40min, and uniformly stirring;
(3) adding a bacterial liquid: adding probiotic fermentation liquor accounting for 3-6% of the weight of the raw materials into the material tank, and uniformly stirring for later use;
(4) adding water: putting the uniformly stirred raw materials in the step (3) into a constant-temperature automatic stirring fermentation container, adding warm water which is 8-10 times of the weight of the raw materials, adding a small amount of water for multiple times, and uniformly stirring;
(5) fermentation: sealing the fermentation container to ensure that the fermentation container is completely sealed and airtight, manufacturing an anaerobic environment, and carrying out anaerobic fermentation, wherein the set temperature of the fermentation container is 35-40 ℃; continuously fermenting for 7-9 days;
(6) and (3) filtering: and (3) after the fermentation time is over, filtering the fermentation product in the fermentation container, combining the filtrates, concentrating under reduced pressure to a relative density of 1.05, sterilizing, adding FN freeze-dried powder according to a proportion, and uniformly stirring to completely dissolve FN, wherein the FN freeze-dried powder can be directly filled and sealed to be used as an oral liquid, or can be frozen into freeze-dried powder by a freeze dryer or spray-dried to be prepared into powder for use.
Fibronectin (FN): is a high molecular glycoprotein, is secreted by vomit cells, widely exists in animal tissues and tissue fluid, and has various biological activities. The molecular weight is about 450KD, and two subunits with the molecular weight of about 230KD are connected through an inter-bond disulfide bond. FN has the physiological effects of promoting phagocytosis of macrophage, promoting cell adhesion, and connecting cells with fiber and matrix. Has close relation with the processes of body wound repair, tissue inflammation, fibrosis, hardening and the like. In the life activity of the organism, FN is involved in the adhesion, spreading, proliferation, migration of cells and development of embryos, and also involved in a plurality of pathological processes of the organism. A number of studies and clinical applications have demonstrated that FN plays a very important role in the repair and healing of wound mucosa, is capable of rapidly repairing damaged gastrointestinal mucosa, and has the efficacy of protecting gastrointestinal mucosa. FN molecules are well conserved during the evolution process, and FN in various animal body fluids has very similar structure, properties and biological functions, so FN from different sources can be used alternatively.
Red dates: chinese herbal medicine books' Benjing records that the red dates have sweet taste and warm property, enter spleen and stomach meridians, and have the functions of tonifying middle-jiao and Qi, nourishing blood and tranquilization and relieving medicine property; modern pharmacology finds that the red dates contain rich nutritional ingredients such as protein, fat, saccharides, organic acid, vitamin A, vitamin C, various trace calcium, and rich polyphenol, flavonoid substances, amino acid and the like. The red date has the effects of tonifying spleen and stomach, benefiting qi and nourishing blood, tranquilizing and allaying excitement and the like, particularly has obvious effects of tonifying spleen and stomach, and also has the effects of enhancing the immunity of organisms, enhancing the muscle strength, increasing the weight, benefiting qi and nourishing blood.
White atractylodes rhizome: floating thistle herb, Yuzhu, Dong Bai Zhu, Thunberg, Yang Fu, Wu Zhu, Ye Zhu, cang Zhu, belonging to Compositae and Atractylodes perennial herb. Pleased in cool climate, has multiple medicinal functions by using rhizome as medicine. Mainly distributed in mountainous wetlands such as Sichuan, Yunnan, Guizhou and the like. The rhizoma atractylodis macrocephalae has the effects of strengthening spleen and tonifying qi, eliminating dampness and promoting diuresis, arresting sweating and preventing miscarriage, and is used for treating spleen deficiency and anorexia, abdominal distension and diarrhea, phlegm and fluid retention and dizziness and palpitation, edema, spontaneous perspiration and threatened abortion. Record of "medical enlightenment": the traditional Chinese medicine composition has the effects of removing dampness and benefiting dryness, regulating the middle warmer and replenishing qi, warming the middle warmer, removing dampness in the spleen and stomach, removing stomach heat, strengthening the spleen and stomach, taking food and preventing miscarriage. "
Glossy privet fruit: the traditional Chinese medicine fructus Ligustri Lucidi is fruit of Ligustrum lucidum belonging to Oleaceae. Modern medicine proves that the glossy privet fruit contains oleanolic acid, privet fruit acid, ligustrazine, ursolic acid, stearic acid, oleic acid, mannitol, betulin, phospholipid, saccharides such as glossy privet fruit polysaccharide, arabose, glucose and the like, and also contains 15 amino acids, 11 trace elements, volatile oil and the like. The research shows that the glossy privet fruit has antibacterial and antiviral effects and has inhibitory effects on staphylococcus aureus, shigella flexneri, typhoid bacillus, pseudomonas aeruginosa and escherichia coli. Has moderate inhibitory effect on B-type adenovirus.
Motherwort: herba Leonuri granule, known as herba Leonuri, is a common Chinese medicine for Labiatae and herba Leonuri plant, and blossoms in summer. The main chemical components of motherwort include alkaloids, diterpenes, flavonoids, various trace elements, fatty acids, etc. Has the effects of removing blood stasis, promoting blood circulation, clearing away heat and toxic materials, inducing diuresis, relieving swelling and pain.
Codonopsis pilosula: the radix Codonopsis is dried root of radix Codonopsis, radix Codonopsis Lanceolatae and other plants belonging to the same genus of Campanulaceae family. Dang Shen is neutral in nature, sweet in flavor and slightly sour. It enters spleen and lung meridians. Invigorating spleen and replenishing qi, invigorating spleen and lung, strengthening body resistance, consolidating constitution, and is suitable for treating spleen and lung weakness, short breath, palpitation, asthma, cough, internal heat, and diabetes. Scientific detection finds that the codonopsis pilosula leaves have the most important value that: has effects in resisting gastric mucosa injury and repairing gastric mucosa, and is related to the effect of radix Codonopsis leaf in enhancing mucosa cytoprotection and gastric mucosa barrier function.
Compared with the prior art, the invention has the following beneficial effects:
(1) the preparation prepared by the invention perfectly combines the traditional Chinese medicines, the probiotics and the FN, can adjust the micro-ecological balance of animal intestinal tracts, purify the intestinal tract environment, improve the immunity of animals, stimulate the regeneration of mucous membrane cells, promote the repair of gastrointestinal mucous membranes, accelerate the recovery of animals infected with colibacillosis, and has the effects of strengthening spleen and kidney, strengthening body resistance and consolidating constitution, and treating both principal and secondary aspects of diseases.
(2) The invention overcomes the defects that the gastrointestinal mucosa repair medicine in the prior art can only temporarily relieve pain, has large side effect and is easy to attack repeatedly. The traditional Chinese medicine composition can quickly repair and damage gastrointestinal mucosa, protect the gastrointestinal mucosa, promote gastrointestinal peristalsis effects, improve the digestion and absorption of animals to nutrient substances, enhance the immune function of an organism, enhance the non-specific immunity of the organism to various bacteria and viruses and resist infection.
(3) According to the invention, the traditional Chinese medicine is subjected to superfine grinding and then is sieved by a 500-mesh sieve, so that the dissolution of active ingredients in the traditional Chinese medicine can be promoted, the fermentation is more thorough, and the prepared oral liquid has higher bioavailability.
(4) The product of the invention has reasonable formula, no drug residue, no drug resistance, high bioavailability, safety and effectiveness and many benefits.
(5) The preparation process disclosed by the invention is green and environment-friendly, is convenient for industrial large-scale production, does not use any organic solvent in the preparation process, does not discharge wastes, and has good economic and social benefits.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A Chinese medicinal preparation for preventing and treating poultry colibacillosis is characterized in that FN (fibronectin) lyophilized powder and fermented traditional Chinese medicine are compounded, wherein the addition amount of the FN lyophilized powder in the fermented traditional Chinese medicine is 0.1% by weight;
the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials in parts by weight: the raw material medicaments: 320 parts of red date, 170 parts of glossy privet fruit, 170 parts of motherwort, 170 parts of white atractylodes rhizome and 170 parts of codonopsis pilosula; probiotic fermentation liquor: the addition amount is 3%.
The probiotic fermentation liquid is a cultured probiotic culture liquid, and the probiotic comprises lactobacillus plantarum, candida utilis, lactobacillus acidophilus and pediococcus acidilactici.
The preparation method comprises the following steps:
(1) superfine grinding: respectively carrying out superfine grinding on various traditional Chinese medicine components in the formula and then sieving the traditional Chinese medicine components with a 500-mesh sieve;
(2) feeding and mixing: accurately weighing the required raw materials which are sieved by a 500-mesh sieve according to the specified proportion, putting the raw materials into a charging bucket, carrying out dry mixing for 20min, and uniformly stirring;
(3) adding a bacterial liquid: adding probiotic fermentation liquor accounting for 3 percent of the weight of the raw materials into the charging bucket, and uniformly stirring for later use;
(4) adding water: putting the uniformly stirred raw materials in the step (3) into a constant-temperature automatic stirring fermentation container, adding warm water which is 8 times of the weight of the raw materials, adding a small amount of water for multiple times, and uniformly stirring;
(5) fermentation: sealing the fermentation container to ensure that the fermentation container is completely sealed and airtight, manufacturing an anaerobic environment, and carrying out anaerobic fermentation, wherein the set temperature of the fermentation container is 35 ℃; continuously fermenting for 7 d;
(6) and (3) filtering: and (3) after the fermentation time is over, filtering the fermentation product in the fermentation container, combining the filtrates, concentrating under reduced pressure to a relative density of 1.05, sterilizing, adding FN freeze-dried powder according to a proportion, and uniformly stirring to completely dissolve FN, wherein the FN freeze-dried powder can be directly filled and sealed to be used as an oral liquid, or can be frozen into freeze-dried powder by a freeze dryer or spray-dried to be prepared into powder for use.
Example 2
A Chinese medicinal preparation for preventing and treating poultry colibacillosis is characterized in that FN (fibronectin) lyophilized powder and fermented traditional Chinese medicine are compounded, wherein the addition amount of the FN lyophilized powder in the fermented traditional Chinese medicine is 2.0% by weight;
the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials in parts by weight: the raw material medicaments: 420 parts of red dates, 145 parts of glossy privet fruits, 145 parts of motherwort, 145 parts of bighead atractylodes rhizome and 145 parts of codonopsis pilosula; probiotic fermentation liquor: the addition amount is 6%.
The probiotic fermentation liquid is a cultured probiotic culture liquid, and the probiotic comprises lactobacillus acidophilus, lactobacillus plantarum, bacillus subtilis and enterococcus faecalis.
The preparation method comprises the following steps:
(1) superfine grinding: respectively carrying out superfine grinding on various traditional Chinese medicine components in the formula and then sieving the traditional Chinese medicine components with a 500-mesh sieve;
(2) feeding and mixing: accurately weighing the required raw materials which are sieved by a 500-mesh sieve according to the specified proportion, putting the raw materials into a charging bucket, carrying out dry mixing for 40min, and uniformly stirring;
(3) adding a bacterial liquid: adding probiotic fermentation liquor accounting for 6 percent of the weight of the raw materials into the charging bucket, and uniformly stirring for later use;
(4) adding water: putting the uniformly stirred raw materials in the step (3) into a constant-temperature automatic stirring fermentation container, adding warm water which is 10 times of the weight of the raw materials, adding a small amount of the warm water for multiple times when adding water, and uniformly stirring;
(5) fermentation: sealing the fermentation container to ensure that the fermentation container is completely sealed and airtight, manufacturing an anaerobic environment, and carrying out anaerobic fermentation, wherein the set temperature of the fermentation container is 40 ℃; continuously fermenting for 9 d;
(6) and (3) filtering: and (3) after the fermentation time is over, filtering the fermentation product in the fermentation container, combining the filtrates, concentrating under reduced pressure to a relative density of 1.05, sterilizing, adding FN freeze-dried powder according to a proportion, and uniformly stirring to completely dissolve FN, wherein the FN freeze-dried powder can be directly filled and sealed to be used as an oral liquid, or can be frozen into freeze-dried powder by a freeze dryer or spray-dried to be prepared into powder for use.
Example 3
A Chinese medicinal preparation for preventing and treating poultry colibacillosis is characterized in that FN (fibronectin) lyophilized powder and fermented traditional Chinese medicine are compounded, wherein the addition amount of the FN lyophilized powder in the fermented traditional Chinese medicine is 1.0% by weight;
the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials in parts by weight: the raw material medicaments: 380 parts of red date, 165 parts of glossy privet fruit, 145 parts of motherwort, 145 parts of white atractylodes rhizome and 165 parts of codonopsis pilosula; probiotic fermentation liquor: the addition amount is 5%.
The probiotic fermentation liquor is a cultured probiotic culture solution, and the probiotics comprise candida utilis, clostridium butyricum and bacillus subtilis.
The preparation method comprises the following steps:
(1) superfine grinding: respectively carrying out superfine grinding on various traditional Chinese medicine components in the formula and then sieving the traditional Chinese medicine components with a 500-mesh sieve;
(2) feeding and mixing: accurately weighing the required raw materials which are sieved by a 500-mesh sieve according to the specified proportion, putting the raw materials into a charging bucket, dry-mixing for 30min, and uniformly stirring;
(3) adding a bacterial liquid: adding probiotic fermentation liquor accounting for 5 percent of the weight of the raw materials into the charging bucket, and uniformly stirring for later use;
(4) adding water: putting the uniformly stirred raw materials in the step (3) into a constant-temperature automatic stirring fermentation container, adding warm water 9 times of the weight of the raw materials, adding a small amount of the warm water for multiple times when adding water, and uniformly stirring;
(5) fermentation: sealing the fermentation container to ensure that the fermentation container is completely sealed and airtight, manufacturing an anaerobic environment, and carrying out anaerobic fermentation, wherein the set temperature of the fermentation container is 38 ℃; continuously fermenting for 8 d;
(6) and (3) filtering: and (3) after the fermentation time is over, filtering the fermentation product in the fermentation container, combining the filtrates, concentrating under reduced pressure to a relative density of 1.05, sterilizing, adding FN freeze-dried powder according to a proportion, and uniformly stirring to completely dissolve FN, wherein the FN freeze-dried powder can be directly filled and sealed to be used as an oral liquid, or can be frozen into freeze-dried powder by a freeze dryer or spray-dried to be prepared into powder for use.
Claims (4)
1. A Chinese medicinal preparation for preventing and treating poultry colibacillosis is characterized in that FN (fibronectin) lyophilized powder and fermented traditional Chinese medicine are compounded, wherein the addition amount of the FN lyophilized powder in the fermented traditional Chinese medicine is 0.1-2.0% by weight;
2. the traditional Chinese medicine preparation for preventing and treating poultry colibacillosis according to claim 1, wherein the fermented traditional Chinese medicine is prepared by mixing and fermenting the following raw materials in parts by weight: the raw material medicaments: 320-420 parts of red date, 170 parts of glossy privet fruit 145-; probiotic fermentation liquor: the addition amount is 3-6%;
3. the Chinese herbal preparation for preventing and treating colibacillosis of poultry according to claim 1, wherein the probiotic fermentation broth is a cultured probiotic culture broth, and the probiotic comprises a mixture of two or more of Clostridium butyricum, Lactobacillus acidophilus, Candida utilis, Lactobacillus plantarum, enterococcus faecalis, Pediococcus acidilactici or Bacillus subtilis;
4. the preparation method of the traditional Chinese medicine preparation for preventing and treating poultry colibacillosis according to claim 1 is characterized by comprising the following steps:
(1) superfine grinding: respectively carrying out superfine grinding on various traditional Chinese medicine components in the formula and then sieving the traditional Chinese medicine components with a 500-mesh sieve;
(2) feeding and mixing: accurately weighing the required raw materials which are sieved by a 500-mesh sieve according to the specified proportion, putting the raw materials into a charging bucket, dry-mixing for 20-40min, and uniformly stirring;
(3) adding a bacterial liquid: adding probiotic fermentation liquor accounting for 3-6% of the weight of the raw materials into the material tank, and uniformly stirring for later use;
(4) adding water: putting the uniformly stirred raw materials in the step (3) into a constant-temperature automatic stirring fermentation container, adding warm water which is 8-10 times of the weight of the raw materials, adding a small amount of water for multiple times, and uniformly stirring;
(5) fermentation: sealing the fermentation container to ensure that the fermentation container is completely sealed and airtight, manufacturing an anaerobic environment, and carrying out anaerobic fermentation, wherein the set temperature of the fermentation container is 35-40 ℃; continuously fermenting for 7-9 days;
(6) and (3) filtering: and (3) after the fermentation time is over, filtering the fermentation product in the fermentation container, combining the filtrates, concentrating under reduced pressure to a relative density of 1.05, sterilizing, adding FN freeze-dried powder according to a proportion, and uniformly stirring to completely dissolve FN, wherein the FN freeze-dried powder can be directly filled and sealed to be used as an oral liquid, or can be frozen into freeze-dried powder by a freeze dryer or spray-dried to be prepared into powder for use.
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Cited By (1)
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