CN112568324B - Composite probiotics fermented traditional Chinese medicine feed additive and preparation method and application thereof - Google Patents
Composite probiotics fermented traditional Chinese medicine feed additive and preparation method and application thereof Download PDFInfo
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- CN112568324B CN112568324B CN202011590563.2A CN202011590563A CN112568324B CN 112568324 B CN112568324 B CN 112568324B CN 202011590563 A CN202011590563 A CN 202011590563A CN 112568324 B CN112568324 B CN 112568324B
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Classifications
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention provides a composite probiotic fermented traditional Chinese medicine feed additive, a preparation method and application thereof, and relates to the field of feed additives. The feed additive is prepared by fermenting a traditional Chinese medicine composition and composite probiotics by using a modern biological fermentation technology, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: radix aucklandiae, pericarpium Citri Tangerinae, fructus Zanthoxyli, glycyrrhrizae radix, rhizoma Alpiniae Officinarum, fructus Ligustri Lucidi, radix astragali, fructus Hippophae, flos Magnoliae officinalis, poria, and fructus Alpinae Oxyphyllae; the composite probiotics comprise: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum and yeast for fermented glutinous rice. The compound probiotic fermented traditional Chinese medicine feed additive provided by the invention can obviously improve the feed intake of broiler chickens, reduce the feed weight ratio and the weight gain effect, effectively reduce the death rate during the test, effectively improve the serum immune antibody level and immune organ index of broiler chickens during the test, and has the beneficial effects of enhancing the immunity and disease resistance of organisms.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a composite probiotic fermented traditional Chinese medicine feed additive, a preparation method thereof and application thereof in the aspect of growth promotion of broiler chickens.
Background
Chicken has the advantages of high protein, low fat, easy digestion and absorption, etc., and has become one of the main meats for residents in China. Along with the continuous improvement of market demands, broiler chicken breeding gradually goes to a large-scale and highly intensive production mode. Meanwhile, the broiler chickens are always in a high-stress and high-sensitivity state in the whole feeding period, so that the production performance and the immunity of the broiler chickens are seriously affected. The additive is added into the feed to promote the growth of broiler chickens and prevent and treat certain diseases, and most of the existing additives are chemical synthetic medicines, antibiotics, hormones and the like, so that the weight of the broiler chickens is increased, meanwhile, the broiler chickens can contain medicine residues, certain toxic and side effects are generated, the health and life safety of human bodies are endangered, and the environment is polluted. With the publication of 194 of the agricultural department, the addition of antibacterial drugs other than Chinese herbal medicines is completely prohibited in the feed of China from 1 month 1 day 2020. Therefore, the search for antibiotic-free green additives for replacing antibiotics becomes a future development direction.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides the compound probiotic fermented traditional Chinese medicine feed additive, and the preparation method and the application thereof.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme:
in one aspect, the invention provides a composite probiotic fermented traditional Chinese medicine feed additive, which comprises the following raw materials in parts by weight: 50-65 parts of traditional Chinese medicine composition, 0.1-1 part of composite probiotic powder, 0-10 parts of soybean meal powder, 0.1-1 part of cellulase, 0-5 parts of brown sugar and 20-40 parts of purified water.
Further, as optimization of the composite probiotic fermented traditional Chinese medicine feed additive, the additive comprises the following raw materials in parts by weight: 55-65 parts of traditional Chinese medicine composition, 0.5-1 part of composite probiotic powder, 5-10 parts of soybean meal powder, 0.1-0.5 part of cellulase, 2-4 parts of brown sugar and 30-35 parts of purified water.
Further, as optimization of the composite probiotic fermented traditional Chinese medicine feed additive, the additive comprises the following raw materials in parts by weight: 62.5 parts of traditional Chinese medicine composition, 1 part of composite probiotic powder, 8 parts of soybean meal powder, 0.5 part of cellulase, 3 parts of brown sugar and 35 parts of purified water.
Further, as optimization of the composite probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 1-10 parts of costustoot, 1-10 parts of orange peel, 1-10 parts of pepper, 1-10 parts of liquorice, 1-10 parts of galangal, 1-10 parts of glossy privet fruit, 1-10 parts of astragalus mongholicus, 1-10 parts of sea buckthorn, 1-10 parts of magnolia officinalis flower, 1-10 parts of poria cocos and 1-10 parts of fructus alpiniae oxyphyllae.
Further, as optimization of the composite probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5-10 parts of costustoot, 5-10 parts of orange peel, 5-10 parts of pepper, 5-10 parts of liquorice, 5-10 parts of galangal, 5-10 parts of glossy privet fruit, 5-10 parts of astragalus mongholicus, 5-10 parts of sea buckthorn, 5-10 parts of magnolia officinalis flower, 5-10 parts of poria cocos and 5-10 parts of fructus alpiniae oxyphyllae.
Further, as optimization of the composite probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Further, as optimization of a composite probiotic fermented traditional Chinese medicine feed additive, the composite probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum, and Saccharomyces cerevisiae; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1-1.5:1.5-2:0.5-1:1.5-2; the number of viable bacteria of each strain is as follows: bacillus licheniformis is more than or equal to 1.5X10 10 CFU/g, pediococcus acidilactici not less than 7.5X10 9 CFU/g, lactobacillus plantarum is more than or equal to 1.0X10 10 CFU/g, saccharomyces cerevisiae not less than 5.5X10% 9 CFU/g。
On the other hand, the invention also provides a preparation method of the composite probiotic fermented traditional Chinese medicine feed additive, which comprises the following steps:
(1) Weighing the following raw materials in parts by weight: 50-65 parts of traditional Chinese medicine composition, 0-10 parts of soybean meal powder, 0-5 parts of brown sugar, 0.1-1 part of cellulase, 0-5 parts of composite probiotic powder and 20-40 parts of purified water;
(2) Dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and hermetically culturing for 24 hours at 37 ℃ to obtain an activated bacterial liquid for later use;
(3) Cleaning, drying and crushing the traditional Chinese medicine composition, sieving with a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use;
(4) And (3) fully mixing and uniformly stirring the activated bacterial liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), placing the mixture in a sealed container, performing anaerobic sealed fermentation at a fermentation temperature of 30-40 ℃ and a fermentation humidity of 30-40% for 3-5 days, and obtaining the composite probiotic fermented traditional Chinese medicine feed additive after fermentation.
The composite strain fermented traditional Chinese medicine feed additive provided by the invention is used for producing broiler chickens and is added into broiler chickens feed according to the standard of 10 kg/T.
The feed additive has the functions of the active ingredients:
radix aucklandiae: pungent and bitter in taste and warm in nature; spleen, stomach, large intestine, triple energizer and gallbladder meridians; has effects of activating qi-flowing, relieving pain, invigorating spleen, and resolving food stagnation; clinically, the traditional Chinese medicine composition is mainly used for treating gastrointestinal diseases such as spleen and stomach qi stagnation syndrome, diarrhea, tenesmus and the like; modern pharmacological researches have shown that radix aucklandiae has effects of relieving smooth muscle spasm, lowering blood pressure, resisting bacteria, etc., and also has certain activity in anticancer, immunity, anti-inflammatory etc.
Orange peel: bitter and pungent taste and warm nature; enter lung and spleen meridians; has effects of regulating qi-flowing, invigorating spleen, eliminating dampness, and eliminating phlegm; is mainly used for treating symptoms such as abdominal distention, anorexia, vomiting and diarrhea, cough and excessive phlegm; from the pharmacological aspect of traditional Chinese medicine, orange peel has the effects of inhibiting gastrointestinal smooth muscle, promoting secretion of digestive juice, promoting bile flow, relaxing bronchial smooth muscle and resisting shock.
Licorice root: sweet in taste and neutral in nature; return to heart, lung, spleen and stomach meridians; has effects of invigorating five viscera, harmonizing various medicines, nourishing kidney, and moistening lung, and contains glycyrrhizic acid, liquiritin, etc. as main ingredients, and can be used for improving weakness of spleen and stomach, listlessness debilitation, and relieving cough and asthma.
Galangal: pungent taste and warm nature; spleen and stomach meridian; has effects in warming stomach, relieving vomiting, dispelling cold, and relieving pain; is mainly used for treating stomach cold pain, stomach cold vomiting, eructation, acid regurgitation and other diseases; the composition is mainly used for treating digestive tract diseases such as dyspepsia, acid regurgitation, emesis, gastric ulcer and the like. The modern researches have separated various effective pharmacological components from galangal, mainly volatile oil, flavonoid and diaryl heptanes, and have outstanding effects in resisting oxidation, resisting ulcer, inhibiting malignant proliferation of tumor, diminishing inflammation and relieving pain.
Fructus ligustri lucidi: sex level; sweet and bitter; enter liver and kidney meridians; has effects in nourishing yin, prolonging life, tonifying liver and kidney, clearing heat, improving eyesight, blackening hair, and blackening hair; the yin-tonifying Chinese herbal medicine has the effects of nourishing liver and kidney and strengthening tendons and bones, is used for treating yin deficiency of liver and kidney, yin deficiency internal heat, weakness of waist and limbs, kidney deficiency and spermatorrhea, can improve the reproductive system function of animals and improve the disease resistance of organisms;
radix astragali: sweet in flavor, slightly warm in smell, thin in smell and strong in flavor, enter lung and spleen meridians. The main functions are as follows: tonify qi, strengthen superficies, expel pus, promote urination and promote tissue regeneration. It is especially effective in invigorating qi and blood and enhancing body's anti-stress and immune functions.
Sea buckthorn: the medicine is prepared from fructus Hippophae, has effects of promoting blood circulation, dispelling blood stasis, eliminating phlegm, relieving chest stuffiness, invigorating spleen, invigorating stomach, promoting salivation, quenching thirst, clearing heat, and relieving diarrhea, and has certain therapeutic effect on various diseases; can be used for treating cough, excessive phlegm, dyspepsia, abdominal pain due to dyspepsia, traumatic injury, blood stasis, swelling, and amenorrhea due to blood stasis; the superoxide dismutase contained in the sea buckthorn fruits can also effectively volatilize in-vivo free radical action, improve the immunity of the organism, regulate immunocompetent cells of the organism and act as an immunomodulator.
Magnolia officinalis flower: bitter in taste, slightly warm in nature, enters spleen and stomach meridians; has the effects of regulating qi-flowing and eliminating dampness, and can be used for treating chest distress, distention, and anorexia.
Poria cocos: sweet and light in taste and neutral in nature; return to heart, lung, spleen and kidney meridians; has effects in promoting diuresis, removing dampness, invigorating spleen, and calming heart; can be used for treating edema, oliguria, phlegm retention, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia; poria cocos is rich in various chemical components, wherein triterpenes and polysaccharide compounds are main active substances of Poria cocos, and modern pharmacological researches show that Poria cocos has pharmacological activities of resisting tumor, regulating immunity, resisting inflammation, resisting oxidation, resisting aging and the like.
Developing intelligence: warm nature, pungent taste, enter spleen and kidney meridians; has effects in warming spleen, relieving diarrhea, removing saliva, warming kidney, and stopping nocturnal emission and urination; the fructus Alpinae Oxyphyllae mainly contains effective components such as sesquiterpenes, dioheptane and flavonoids, and has pharmacological effects of regulating urination, resisting bacteria, resisting tumor and resisting oxidative stress.
Bacillus licheniformis: the strain is a functional feed microorganism which is bred by a modern biotechnology and a special process. After entering the intestinal tract, the living bacteria can make the intestinal tract lack oxygen through oxygen-capturing biological effect, and has inhibition effect on pathogenic bacteria such as staphylococcus, escherichia coli and the like; the microbial agent has the effect of promoting growth of bifidobacteria, lactobacillus, bacteroides and digestive streptococcus, thereby achieving the purposes of adjusting the imbalance of intestinal flora and maintaining the microecological balance of the intestinal tract of the organism.
Pediococcus acidilactici: the bacterial strain is a gram positive bacterium for producing acid and bacteriocin, can effectively regulate the gastrointestinal tract health of animals, maintain the balance of intestinal flora, has the effect of antagonizing pathogenic microorganisms in animals, can competitively inhibit the pathogenic microorganisms, and enhances the immune function of the animals, and is a novel feeding microorganism.
Lactobacillus plantarum: the strain has strong lactic acid production capability and relatively strong lactic acid production capability. Can effectively inhibit the growth of pathogenic bacteria, promote the peristalsis of animal intestinal tracts and improve the digestion and absorption of feed.
Saccharomyces cerevisiae: also known as baker's yeast or budding yeast. The propagation method is budding reproduction, and has the advantages of short growth period, strong fermentation capacity, easiness in large-scale culture, and rich nutrition components including various proteins, amino acids, vitamins, bioactive substances and the like because of easy culture. Saccharomyces cerevisiae belongs to facultative anaerobes, and after entering the gastrointestinal tract of animals, oxygen in the gastrointestinal tract can be consumed to cause an anaerobic environment, so that the propagation of beneficial bacteria is promoted, and the microecological balance of the digestive tract of the animals is improved.
(III) beneficial effects
The invention provides a compound probiotic fermented traditional Chinese medicine feed additive, a preparation method thereof and application thereof in production of broiler chickens, wherein the feed additive is prepared by fermenting a traditional Chinese medicine composition and compound probiotics by utilizing a modern biological fermentation technology. After the traditional Chinese medicine composition is fermented by probiotics, the plant cell walls of the traditional Chinese medicine composition can be further opened, the pre-digestion effect is achieved, the digestion time of the medicine in the body is reduced, and the absorption and utilization rate of the medicine is improved; meanwhile, a large amount of active beneficial bacteria and unknown growth factors can be generated in the fermentation process, and the method is more beneficial to stabilizing the intestinal microecological balance and health of organisms. The compound probiotic fermented traditional Chinese medicine feed additive provided by the invention can obviously improve the feed intake of broiler chickens, reduce the feed weight ratio and has obvious weight increasing effect, can effectively improve the serum immune antibody level and immune organ index of broiler chickens during a test period, and has the beneficial effects of enhancing the immunity and disease resistance of organisms. The feed additive disclosed by the invention is convenient to operate in the production process, safe and environment-friendly, is a green antibiotic-free product, and can be widely applied to the cultivation of broilers.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A composite probiotic fermented traditional Chinese medicine feed additive is prepared according to the following steps:
(1) Weighing the following raw materials in parts by weight: 50 parts of traditional Chinese medicine composition, 2 parts of soybean meal, 1 part of brown sugar, 0.1 part of cellulase, 0.1 part of composite probiotic powder and 25 parts of purified water;
(2) Dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and hermetically culturing for 24 hours at 37 ℃ to obtain an activated bacterial liquid for later use; wherein the probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum, and Saccharomyces cerevisiae; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:0.5:1.5; the number of viable bacteria of each strain is as follows: bacillus licheniformis is not less than1..5×10 10 CFU/g, pediococcus acidilactici not less than 7.5X10 9 CFU/g, lactobacillus plantarum is more than or equal to 1.0X10 10 CFU/g, saccharomyces cerevisiae not less than 5.5X10% 9 CFU/g。
(3) Cleaning, drying and crushing the traditional Chinese medicine composition, sieving with a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 6 parts of costustoot, 4 parts of orange peel, 2 parts of pepper, 4 parts of liquorice, 6 parts of galangal, 8 parts of glossy privet fruit, 5 parts of astragalus mongholicus, 4 parts of sea buckthorn, 2 parts of magnolia officinalis flower, 4 parts of poria cocos and 4 parts of fructus alpiniae oxyphyllae.
(4) And (3) fully mixing and uniformly stirring the activated bacterial liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), placing the mixture in a sealed container, performing anaerobic sealed fermentation at a fermentation temperature of 30-40 ℃ and a fermentation humidity of 30-40% for 3-5 days, and obtaining the composite probiotic fermented traditional Chinese medicine feed additive after fermentation.
Example 2
This embodiment differs from embodiment 1 in that:
the composite probiotics are composed of the strains according to the following weight ratio: bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1:2:1:2;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10 parts of costustoot, 10 parts of orange peel, 10 parts of pepper, 10 parts of liquorice, 10 parts of galangal, 10 parts of glossy privet fruit, 10 parts of astragalus mongholicus, 10 parts of sea buckthorn, 1-10 parts of magnolia officinalis flower, 10 parts of poria cocos and 10 parts of fructus alpiniae oxyphyllae.
Example 3
This embodiment differs from embodiment 1 in that:
the composite probiotics are composed of the strains according to the following weight ratio: bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.2:1.8:0.8:1.2;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of costustoot, 5 parts of orange peel, 5 parts of pepper, 5 parts of liquorice, 5 parts of galangal, 5 parts of glossy privet fruit, 5 parts of astragalus mongholicus, 5 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 4
This embodiment differs from embodiment 1 in that:
the composite probiotics are composed of the strains according to the following weight ratio: bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:1:1.5;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 5
This embodiment differs from embodiment 4 in that:
weighing the following raw materials in parts by weight: 65 parts of traditional Chinese medicine composition, 1 part of composite probiotic powder, 10 parts of soybean meal powder, 1 part of cellulase, 5 parts of brown sugar and 40 parts of purified water.
Example 6
This embodiment differs from embodiment 4 in that:
weighing the following raw materials in parts by weight: 55 parts of traditional Chinese medicine composition, 0.5 part of composite probiotic powder, 5 parts of soybean meal powder, 0.1 part of cellulase, 4 parts of brown sugar and 35 parts of purified water.
Example 7
This embodiment differs from embodiment 4 in that:
weighing the following raw materials in parts by weight: 62.5 parts of traditional Chinese medicine composition, 1 part of composite probiotic powder, 8 parts of soybean meal powder, 0.5 part of cellulase, 3 parts of brown sugar and 35 parts of purified water.
Comparative example 1
The difference between this comparative example and example 7 is that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower and 5 parts of poria cocos.
Comparative example 2
The difference between this comparative example and example 7 is that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 6 parts of liquorice, 10 parts of glossy privet fruit, 8 parts of astragalus, 5 parts of magnolia officinalis, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 3
The difference between this comparative example and example 7 is that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 5
The difference between this comparative example and example 7 is that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of sea buckthorn, 5 parts of magnolia officinalis, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 6
The difference between this comparative example and example 7 is that:
the composite probiotics are composed of the strains according to the following weight ratio: bacillus licheniformis: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5.
Comparative example 7
The difference between this comparative example and example 7 is that:
the composite probiotics are composed of the strains according to the following weight ratio: bacillus licheniformis: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:1.5.
Comparative example 8
The difference between this comparative example and example 7 is that:
the composite probiotics are composed of the strains according to the following weight ratio: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1:1.5.
Comparative example 9
Cleaning, drying and crushing the traditional Chinese medicine composition, and sieving the traditional Chinese medicine composition with a 60-mesh sieve to obtain the traditional Chinese medicine additive, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 8
According to the traditional Chinese medicine theory and the modern fermentation technology, the formula is screened and researched by combining the growth characteristics of the broiler chickens, and the broiler chickens feeding test effects are compared by combining different formulas, so that the components of the feed additive are finally obtained.
1 test method
1.1 design of experiments
500 Eveline broilers with 1 ration are selected and randomly divided into 10 groups, namely a test group, a comparison group 1 and a comparison group 9, and 50 broilers are fed to each group, wherein the test group is fed with the fermented traditional Chinese medicine feed additive prepared in example 7, the comparison groups 1 to 9 are respectively fed with the feed additives prepared in the comparison examples 1 to 9, basic ration plus 1% of feed additive is adopted, free feeding and free drinking water are realized during the test, the consistency of feeding environment is ensured, and the test is carried out for 42 days.
2 measurement of production Properties
Weight index 42 days of age 19:00 stopped water supply, 43 days of age 08:00, all repeated weighing. Feed intake of broilers in each repetition of each group per day was recorded, and daily average feed intake (ADFI), average Daily Gain (ADG) feed-to-meat ratio (F/G) were calculated.
3 statistical analysis of data
Data analysis all data were statistically tested using SPSS20.0, with results expressed as mean ± standard deviation, and differences were significant as (P < 0.05).
4 results and analysis
Table 1 shows the results of the production performance measurement of each group of test broiler chickens after 42 days of the test. As can be seen from Table 1, the test groups were higher than the comparative 1-9 groups in terms of daily gain and feed intake; the weight ratio of the materials in the test group is lower than that in the comparison group 1-9, and the daily gain is improved but the effect is not obvious when any raw material in the invention is absent or any strain in the invention is absent. Thus, the additive formulation of the test group is the preferred formulation of the present invention.
TABLE 1 influence of different formulations of feed additives on the production performance of 1-42 day old broilers
| Grouping | Daily weight gain/g | Daily average feed intake/g | Ratio of material to weight |
| Test group | 55.03±1.30 | 100.21±7.52 | 1.82 |
| Comparative group 1 | 54.22±1.21 | 99.37±6.23 | 1.85 |
| Comparative group 2 | 53.13±1.23 | 98.26±6.01 | 1.85 |
| Comparative group 3 | 51.33±1.09 | 95.37±5.98 | 1.86 |
| Comparative group 4 | 52.02±1.11 | 97.22±6.29 | 1.87 |
| Comparative group 5 | 52.79±1.46 | 99.15±6.55 | 1.88 |
| Comparative group 6 | 50.83±1.18 | 96.55±5.77 | 1.90 |
| Comparative group 7 | 51.09±1.02 | 97.13±6.03 | 1.90 |
| Comparison of group 8 | 51.44±1.05 | 98.33±6.89 | 1.91 |
| Comparative group 9 | 49.37±1.09 | 95.36±6.21 | 1.93 |
Example 9
To further illustrate the effect of the fermented traditional Chinese medicine feed additive of the present invention on the productivity and immunity of broilers, animal tests are provided below as an illustration. The fermented traditional Chinese medicine feed additives according to the invention are prepared according to example 7.
1 test method
Selecting 600 Evelin chickens with 1 ration, randomly dividing into 3 groups, namely a blank group, a control group, a test group, and 5 repetitions of each group, wherein 40 repetitions of each group; wherein the blank group is only fed with basic ration; the control group adds 20% chlortetracycline hydrochloride into the basic ration, the addition amount is 200 g/ton; test group feeding the fermented traditional Chinese medicine feed additive prepared in example 7 adopts basic ration plus 1% of feed additive. The test period adopts on-net flat raising, only feeding and drinking water are adopted, longitudinal negative pressure ventilation is realized, a hot blast stove is used for heating, and raising management and immunization programs refer to Evelin broiler raising management manual.
2 index measurement and measurement method
2.1 measurement of production Properties
Weight index 42 days of age 19:00 stopped water supply, 43 days of age 08:00, all repeated weighing. Feed intake of broilers in each repetition of each group per day was recorded, and daily average feed intake (ADFI), average Daily Gain (ADG) feed-to-meat ratio (F/G) were calculated.
2.2 measurement of serum immune index and visceral index
The water supply was stopped at 19:00 of age 42 days, the vein was collected and slaughtered (2 replicates each) at 08:00 of age 43 days, serum immune indicators SigA, igM, igA were measured, thymus, spleen, bursa weight were weighed, and organ indices were calculated.
Organ index = organ weight (g)/body weight (g) ×100%
3 data statistics and analysis
Data analysis all data were statistically tested using SPSS20.0, with results expressed as mean ± standard deviation, and differences were significant as (P < 0.05).
4 results and analysis
4.1 Effect of the fermented traditional Chinese medicine feed additive of the invention on the productivity of broiler chickens
As can be seen from table 2, the final weight of the test group was significantly increased (P < 0.05) compared to the control group; compared with the control group, the method has a certain improvement (P is more than 0.05). Compared with the blank group, the daily gain of the test group is significantly higher than that of the blank group (P < 0.05) and the control group (P < 0.05), and the difference between the blank group and the control group is not significant. The difference between the groups of daily average feed intake is not obvious (P > 0.05). The experimental group had a significantly higher feed weight ratio than the blank group (P < 0.05) and the control group (P < 0.05), and the difference between the blank group and the control group was not significant.
TABLE 2 influence of the fermented Chinese medicinal feed additive of the invention on the productivity of 1-42 day-old broilers
Note that the same row of data shoulder marks with different lower case letters represent significant differences (P < 0.05), as follows.
4.2 Effect of the fermented traditional Chinese medicine feed additive of the invention on serum antibody level of broiler chickens
As can be seen from table 3, the test group SigA content was significantly higher than the blank group (P < 0.01), and the test group SigA was significantly higher than the control group (P < 0.05); control SigA content was significantly higher than the blank autonomy (P < 0.05). Compared with the blank group, the IgG content of the test group is significantly higher than that of the blank group (P < 0.05) and than that of the control group (P > 0.05); control IgG was higher than blank (P > 0.05). Compared with the blank group, the IgM content of the test group is significantly higher than that of the blank group (P < 0.05), the control group (P < 0.05), and the difference between the blank group and the control group is not significant.
TABLE 3 Effect of the fermented traditional Chinese medicine feed additives of the invention on serum antibody levels of 1-42 day old broilers
4.3 Effect of the fermented traditional Chinese medicine feed additive of the invention on the immune organ index of broilers
As can be seen from table 4, the thymus index of the test group was significantly higher than that of the blank group (P < 0.05); the thymus index of the test group is not significantly different from that of the control group (P > 0.05); the difference between the blank and control groups was not significant (P > 0.05). Thymus index, differences between groups were not significant (P > 0.05). Compared with the blank group, the bursa of Fabricius index of the test group is obviously higher than that of the blank group; the bursa index of the test group was significantly higher than that of the control group (P < 0.05); the difference between the blank and control groups was not significant P > 0.05).
TABLE 4 influence of the fermented Chinese medicinal feed additive of the invention on immune organ index of 1-42 day old broiler chickens
According to the test results, the fermented traditional Chinese medicine feed additive provided by the invention can be used for feeding broilers, can effectively reduce the feed weight ratio, increase daily feed intake, promote daily weight gain, has positive influence on the production of broilers, can increase the antibody level of broiler serum, promote the immune organ index of broilers, replace the use of antibiotics, enhance the immune function of broilers and promote the disease resistance.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (1)
1. The composite probiotic fermented traditional Chinese medicine feed additive is characterized by comprising the following raw materials in parts by weight: 62.5 parts of traditional Chinese medicine composition, 1 part of composite probiotic powder, 0.5 part of cellulase, 8 parts of soybean meal powder, 3 parts of brown sugar and 35 parts of purified water; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos, and 5 parts of fructus alpiniae oxyphyllae; the composite probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum, and Saccharomyces cerevisiae; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:1:1.5; the number of viable bacteria of each strain is as follows: bacillus licheniformis is more than or equal to 1.5X10 10 CFU/g, pediococcus acidilactici not less than 7.5X10 9 CFU/g, lactobacillus plantarum is more than or equal to 1.0X10 10 CFU/g, saccharomyces cerevisiae not less than 5.5X10% 9 CFU/g; the feed is provided withThe material additive is prepared according to the following steps:
(1) Weighing the following raw materials in parts by weight: 62.5 parts of traditional Chinese medicine composition, 1 part of composite probiotic powder, 0.5 part of cellulase, 8 parts of soybean meal powder, 3 parts of brown sugar and 35 parts of purified water;
(2) Dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and hermetically culturing for 24 hours at 37 ℃ to obtain an activated bacterial liquid for later use;
(3) Cleaning, drying and crushing the traditional Chinese medicine composition, sieving with a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use;
(4) And (3) fully mixing and uniformly stirring the activated bacterial liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), placing the mixture in a sealed container, performing anaerobic sealed fermentation at a fermentation temperature of 30-40 ℃ and a fermentation humidity of 30-40% for 3-5 days, and obtaining the composite probiotic fermented traditional Chinese medicine feed additive after fermentation.
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| CN115152893A (en) * | 2022-07-04 | 2022-10-11 | 广西农业职业技术大学 | Compound probiotic composition for Qingyuan spicy-feather chicks and preparation method thereof |
| CN115669802B (en) * | 2022-08-23 | 2024-01-26 | 辽宁九州生物科技有限公司 | Traditional Chinese medicine composition and feed additive for improving anti-stress capability as well as preparation methods and application thereof |
| CN117256731A (en) * | 2023-09-14 | 2023-12-22 | 中国热带农业科学院热带生物技术研究所 | Black soldier fly larva fermentation liquor, preparation method and application thereof, and shrimp feed |
| CN117837679B (en) * | 2024-02-20 | 2024-07-05 | 江西省农业科学院畜牧兽医研究所 | Chicken feed additive based on moringa oleifera and preparation method thereof |
| CN118058390A (en) * | 2024-04-12 | 2024-05-24 | 山东广元药业科技有限公司 | Feed additive for enhancing animal immunity and antiviral ability and preparation method thereof |
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