CN111789130A - Plant growth regulator and application and use method thereof - Google Patents

Plant growth regulator and application and use method thereof Download PDF

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Publication number
CN111789130A
CN111789130A CN202010761202.3A CN202010761202A CN111789130A CN 111789130 A CN111789130 A CN 111789130A CN 202010761202 A CN202010761202 A CN 202010761202A CN 111789130 A CN111789130 A CN 111789130A
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plant growth
growth regulator
milk vetch
spraying
parts
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CN111789130B (en
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唐先干
徐昌旭
李祖章
刘增兵
孙刚
张文学
袁福生
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Institute of Soil Fertilizer Resources and Environment of Jiangxi Academy of Agricultural Sciences
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Institute of Soil Fertilizer Resources and Environment of Jiangxi Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Forests & Forestry (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Ecology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of plant growth regulation, and particularly relates to a plant growth regulator and an application and using method thereof. The plant growth regulator comprises nano silicon dioxide, graphene oxide and salicylic acid, is applied to planting of the milk vetch, and can be applied to the seedling stage and the green turning stage of the milk vetch to improve the nitrogen fixation capacity of the root nodule of the milk vetch and the yield of the milk vetch.

Description

Plant growth regulator and application and use method thereof
Technical Field
The invention belongs to the technical field of plant growth regulation, and particularly relates to a plant growth regulator and an application and using method thereof.
Background
Astragalus sinicus (scientific name: Astragalus sinicus L.) and safflower are plants of Astragalus of Leguminosae. The milk vetch can improve and renew soil organic matters, enrich soil phosphorus and potassium, increase biological coverage rate, has outstanding fertility improvement effect, improves soil physical and chemical properties, promotes crop growth, increases soil microorganism quantity and diversity, improves soil ecological environment, promotes soil organic carbon accumulation, reduces CO2The astragalus sinicus green manure plays an irreplaceable role today when low-carbon agriculture and organic concentration are advocated.
The milk vetch as a green fertilizer does not need to be fertilized in a large quantity, but a small quantity of phosphorus-potassium fertilizer, especially a phosphate fertilizer, is applied in the early stage, so that nodulation can be promoted, phosphorus is added to increase nitrogen, and the purpose of culturing a large fertilizer with a small fertilizer is achieved. The source of the organic nitrogen of the milk vetch mainly comes from the nitrogen fixation of the root nodules, the nitrogen fixation amount accounts for 80 percent of the total nitrogen amount of plants under the condition of good nodulation, and the biological nitrogen fixation of the milk vetch can save huge energy and industrial investment every year. The application of the milk vetch can reduce the application amount of the inorganic nitrogen fertilizer and reduce the risk of environmental pollution. However, in order to further improve the growth, seed retention and nitrogen fixation capacity of the milk vetch, a method of spraying a growth regulator on the leaf surface of the milk vetch is mostly adopted, the commonly used growth regulator is a paclobutrazol regulator at present, but the improvement of the nitrogen fixation capacity of the milk vetch by the regulator needs to be further improved.
Disclosure of Invention
Therefore, the invention aims to overcome the defects that the nitrogen fixation capacity of the astragalus sinicus needs to be further improved and the like in the prior art, and provides a plant growth regulator, and an application and a use method thereof.
Therefore, the invention provides the following technical scheme.
The invention provides a plant growth regulator which comprises, by weight, 5-15 parts of nano silicon dioxide, 10-30 parts of graphene oxide and 2-4 parts of salicylic acid.
Further, the plant growth regulator comprises, by weight, 8-11 parts of nano silicon dioxide, 18-23 parts of graphene oxide and 3 parts of salicylic acid.
Further, the plant growth regulator comprises 10 parts of nano silicon dioxide, 20 parts of graphene oxide and 3 parts of salicylic acid in parts by weight.
The mass concentration of the plant growth regulator is 150-500 mg/L.
The mass concentration of the plant growth regulator is 150-200 mg/L.
The invention also provides an application of the plant growth regulator in planting of the violaxanthin.
In addition, the invention also provides application of the plant growth regulator in nitrogen fixation of astragalus sinicus.
Further, the invention also provides a using method of the plant growth regulator, which comprises the following steps:
(1) in the seedling stage of the plant, the plant growth regulator is sprayed on the leaf surfaces of the plant for 2 to 4 times, and the interval time between two adjacent spraying times is 2 to 4 days;
(2) in the plant green-turning period, the plant growth regulator is sprayed on the leaf surfaces of the plants for 2-4 times, and the interval time between two adjacent spraying times is 2-4 days.
In the step (1), the single dosage of the sprayed plant growth regulator is 29-30.5L/mu;
in the step (2), the single dosage of the sprayed plant growth regulator is 29-30.5L/mu.
The technical scheme of the invention has the following advantages:
1. the plant growth regulator provided by the invention comprises nano silicon dioxide, graphene oxide and salicylic acid, and the synergistic effect of the nano silicon dioxide, the graphene oxide and the salicylic acid can promote the formation of the root nodule of the milk vetch in the seedling stage of the leguminous plant, improve the photosynthesis and the nitrogen fixation capacity of the root nodule of the milk vetch of the leguminous plant, and simultaneously promote the absorption and utilization of the root nodule bacteria on the dihydroxy acid with four carbon atoms, so that the nitrogen fixation capacity of the root nodule of the milk vetch is further improved; the nano silicon dioxide has the characteristics of large specific surface area, high surface hydroxyl content and strong adsorption capacity, and can promote the nitrogen fixation capacity of the astragalus sinicus rhizobium; the graphene oxide is beneficial to uniformly dispersing the nano silicon dioxide in an aqueous solution, and can form a layer of uniform protective film on the surface of a plant leaf, and the protective film has the effects of antibiosis, water permeability, light transmittance and the like and is beneficial to enhancing the nitrogen fixation capacity of the nodule; the salicylic acid can obviously improve the photosynthetic property of the astragalus sinicus, improve the yield of the astragalus sinicus, and promote the rhizobia to absorb the four-carbon dihydroxy acid and the nitrogen fixation capacity of the astragalus sinicus rhizobia.
2. The plant growth regulator provided by the invention limits the mixture ratio of the components of the plant growth regulator, and can obviously improve the nitrogen fixation capacity of astragalus sinicus.
3. The plant growth regulator provided by the invention is applied to planting of milk vetch, and the nitrogen fixation capacity of the root nodules and the yield of the milk vetch can be improved by applying the plant growth regulator provided by the invention to the seedling stage and the green turning stage of the milk vetch.
4. The application method of the plant growth regulator provided by the invention is simple and feasible, has small operation difficulty and is easy to realize large-area application.
Example 1
The embodiment provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 2
The embodiment provides a plant growth regulator, which comprises 50mg of nano silicon dioxide, 300mg of graphene oxide and 20mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 3
The embodiment provides a plant growth regulator, which comprises 150mg of nano silicon dioxide, 100mg of graphene oxide and 40mg of solvent, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 4
The embodiment provides a plant growth regulator, which comprises 90mg of nano silicon dioxide, 230mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 5
The embodiment provides a plant growth regulator, which comprises 140mg of nano silicon dioxide, 280mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 6
The embodiment provides a plant growth regulator, which comprises 60mg of nano silicon dioxide, 150mg of graphene oxide and 25mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 7
The embodiment provides a plant growth regulator, which comprises 105mg of nano silicon dioxide, 190mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 8
The embodiment provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 180 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Example 9
The embodiment provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 350 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Comparative example 1
The comparative example provides a plant growth regulator, which comprises 100mg of nano silicon dioxide and 200mg of graphene oxide, the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Comparative example 2
The comparative example provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of lysine, the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Comparative example 3
The comparative example provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of glutamic acid, the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Comparative example 4
The comparative example provides a plant growth regulator, which comprises 100mg of nano silicon dioxide, 200mg of graphene oxide and 30mg of salicylic acid, wherein the solvent is water, and the mass concentration of the plant growth regulator is 100 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Comparative example 5
The comparative example provides a plant growth regulator, which comprises 20mg of nano silicon dioxide, 400mg of graphene oxide and 30mg of salicylic acid, the solvent is water, and the mass concentration of the plant growth regulator is 150 mg/L.
The application of the plant growth regulator in planting the astragalus sinicus, and the using method of the plant growth regulator comprises the following steps:
in the seedling stage of the milk vetch, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu;
and (3) in the period of turning green of the milk vetch, spraying the plant growth regulator on the milk vetch leaf surfaces for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu.
Test example 1
This experimental example provides performance tests of the plant growth regulators provided in examples 1 to 9, specifically as follows:
in 2017, in 10 months, astragalus sinicus is planted in a test base of Chongren county in Jiangxi province, in 12 months in the same year, the seedling stage of the astragalus sinicus is started, 2 times of plant growth regulators are sprayed on the leaf surfaces of the astragalus sinicus, the interval time of spraying the plant growth regulators twice is 3 days, and the single dosage is 30L/mu; entering the green turning period of the milk vetch in 2 months of 2018, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu; and harvesting the astragalus sinicus from 4 months to the full-bloom stage of the astragalus sinicus in 2018.
Testing the overground fresh grass amount, the nitrogen content and the nitrogen fixation amount of the astragalus sinicus, wherein the testing method of the overground fresh grass amount comprises the following steps: randomly taking 3 sample squares of 2m multiplied by 2m to harvest the milk vetch, weighing, and calculating the average value which is the amount of the fresh grass on the overground part, wherein the unit is; kg/hm2
The method for testing the nitrogen content of the fresh grass comprises the following steps: taking milk vetch, menstruation H2SO4-H2O2After digestion, the nitrogen content of the fresh grass is measured by an automatic nitrogen determination instrument method, and the nitrogen content of the fresh grass is obtained, wherein the unit is g/kg.
The method for testing the nitrogen fixation amount comprises the following steps: the amount of fresh herb on the ground is multiplied by the nitrogen content of milk vetch, and the unit is kg/hm2
TABLE 1 results of the effect of different plant growth regulators on Astragalus sinicus
Figure BDA0002613125320000101
Remarking: the control group in table 1 was water; the same column of numerical values indicates that the different letters indicate that the difference is significant (P < 0.05).
In table 1, after the plant growth regulator provided by the present invention is sprayed in the seedling stage and the seedling turning stage of milk vetch, the amount of fresh grass, the nitrogen content of fresh grass and the nitrogen fixation amount of milk vetch can be increased. The plant growth regulator is characterized in that the ratio of the components of the plant growth regulator is limited to a specific numerical value, so that the root nodule number, the fresh grass content, the nitrogen content and the nitrogen fixation amount of the astragalus sinicus can be further increased, and particularly, when the weight parts of the nano silicon dioxide, the graphene oxide and the salicylic acid in the plant growth regulator are respectively 10 parts, 20 parts and 3 parts, the nitrogen fixation capacity of the astragalus sinicus is improved most obviously. Compared with a control group, the plant growth regulator provided by the embodiment 1 of the invention has the advantages that the number of root nodules is increased by 7.4%, the amount of the astragalus sinicus fresh grass is increased by 5.1%, the nitrogen content of the fresh grass is increased by 9.7%, and the total nitrogen fixation amount is increased by 15.3%, and the plant growth regulator is obtained by calculation according to a formula I.
Figure BDA0002613125320000111
Test example 2
The test example also provides performance tests of the plant growth regulators provided in example 1 and comparative examples 1 to 5, which specifically include the following:
in 2018, in 10 months, planting milk vetch in a test base of Chongren county in Jiangxi province, in 12 months in the same year, entering a milk vetch seedling stage, spraying the plant growth regulator on the leaf surfaces of the milk vetch for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu; entering the green turning period of the milk vetch in 2 months of 2019, spraying the plant growth regulator on the surfaces of the milk vetch leaves for 2 times, wherein the interval time of spraying the plant growth regulator for two times is 3 days, and the single dosage is 30L/mu; and harvesting the astragalus sinicus from 4 months to the full-bloom stage of the astragalus sinicus in 2019.
The overground fresh grass amount, the fresh grass nitrogen content and the nitrogen fixation amount of the astragalus sinicus are tested, the testing method is the same as that of the test example 1, and the testing results are shown in a table 2.
TABLE 2 results of the effect of different plant growth regulators on Astragalus sinicus
Figure BDA0002613125320000112
Remarking: the control group in table 1 was water; the same column of numerical values indicates that the different letters indicate that the difference is significant (P < 0.05).
Example 1 compared with comparative examples 1, 2 and 3, the plant growth regulator is added with salicylic acid, and can improve the nitrogen content, the root nodule number and the nitrogen fixation amount of fresh grass of a plant and improve the nitrogen fixation capacity of astragalus sinicus; the nitrogen fixation ability of milk vetch cannot be improved by adding lysine or glutamic acid to the plant growth regulator. Example 1 shows that, when the ratio of nanosilicon dioxide, graphene oxide and salicylic acid is outside the range defined by the present invention, the plant growth regulator can improve the nitrogen fixation ability of milk vetch, but the effect is poor, compared to comparative examples 4 and 5.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the spirit or scope of the invention.

Claims (9)

1. A plant growth regulator is characterized by comprising, by weight, 5-15 parts of nano silicon dioxide, 10-30 parts of graphene oxide and 2-4 parts of salicylic acid.
2. The plant growth regulator according to claim 1, comprising 8-11 parts of nano silicon dioxide, 18-23 parts of graphene oxide and 3 parts of salicylic acid by weight.
3. The plant growth regulator according to claim 1 or 2, comprising 10 parts of nanosilica, 20 parts of graphene oxide and 3 parts of salicylic acid.
4. The plant growth regulator according to any one of claims 1-3, wherein the mass concentration of the plant growth regulator is 150-500 mg/L.
5. The plant growth regulator according to claim 4, wherein the mass concentration of the plant growth regulator is 150-200 mg/L.
6. Use of the plant growth regulator of any one of claims 1 to 5 for planting of violaxanthin.
7. Use of the plant growth regulator of any one of claims 1-5 for nitrogen fixation of Astragalus sinicus.
8. Use of a plant growth regulator according to any of claims 1 to 5, characterized in that it comprises the following steps:
(1) in the seedling stage of the plant, the plant growth regulator is sprayed on the leaf surfaces of the plant for 2 to 4 times, and the interval time between two adjacent spraying times is 2 to 4 days;
(2) in the plant green-turning period, the plant growth regulator is sprayed on the leaf surfaces of the plants for 2-4 times, and the interval time between two adjacent spraying times is 2-4 days.
9. The use method according to claim 8, wherein in the step (1), the single dosage of the sprayed plant growth regulator is 29-30.5L/mu;
in the step (2), the single dosage of the sprayed plant growth regulator is 29-30.5L/mu.
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