CN111763127A - Preparation method for producing fresh earthworm full-nutrient fermented liquid fertilizer in large scale - Google Patents
Preparation method for producing fresh earthworm full-nutrient fermented liquid fertilizer in large scale Download PDFInfo
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- CN111763127A CN111763127A CN202010014868.2A CN202010014868A CN111763127A CN 111763127 A CN111763127 A CN 111763127A CN 202010014868 A CN202010014868 A CN 202010014868A CN 111763127 A CN111763127 A CN 111763127A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a preparation method for producing a fresh earthworm total nutrient fermented liquid fertilizer in a large scale, which belongs to the field of earthworm fermented liquid fertilizers and comprises the following steps: s1: breeding earthworms to obtain living earthworms, S2: extracting zymophyte from earthworms and completing the screening of zymophyte, S3: carrying out fermentation, S4: and (4) after the fermentation is finished, performing solid-liquid separation, wherein S5: collecting the liquid obtained in the step S4 to obtain the earthworm total nutrient fermented liquid fertilizer, and performing S6: and packaging the obtained earthworm total nutrient fermented liquid fertilizer. The fresh earthworm total nutrient fermented liquid fertilizer produced by the invention belongs to an organic fertilizer, has comprehensive nutrients, is rich in organic matters, humic acid and microbial flora, accelerates microbial propagation, is beneficial to the absorption of nutrients by plants, is harmless to plants, people and livestock, can enhance the resistance of the plants to plant diseases and insect pests, inhibits plant soil-borne diseases, improves the quality of the crops, recovers the natural flavor of the crops, and avoids the pollution of the traditional compound fertilizer to the environment.
Description
Technical Field
The invention relates to the field of earthworm fermented liquid fertilizers, in particular to a preparation method for producing a fresh earthworm full-nutrient fermented liquid fertilizer in a large scale.
Background
Chemical fertilizers are essential in modern agricultural production, but the problems of microbial activity reduction, soil degradation and the like caused by the use of the chemical fertilizers make people pay attention to chemical fertilizer pollution. The fertilizer pollution is a phenomenon of water, soil and atmosphere pollution caused by applying a large amount of fertilizer to farmlands. And moreover, excessive use of agricultural chemicals causes lack of organic matters and humus, soil structure is damaged, soil nutrient structure is disordered, soil is hardened, environment pollution is caused, nutrients cannot be effectively absorbed and utilized by crops, crop yield is reduced, and the like, so that organic fertilizers are paid more and more attention.
The earthworms are the best researched organic fertilizers, are high-quality biological organic fertilizers with soft bodies, high protein content and organic wastes which like humic substances, contain various amino acids, enzymes and active substances in bodies, and have good economic value and medicinal value.
However, in the prior art, the earthworm organic fertilizer is mainly a solid fertilizer, so that crops absorb slowly, nutrients are easy to lose, and the utilization rate is far lower than expected. The living earthworms are rich in various nutrient elements for plant growth, and the production of the fresh earthworm full-nutrient fermented liquid fertilizer by using the living earthworms becomes one of the important ways for developing organic fertilizers in the future.
Compared with the preparation method for producing the fresh earthworm total nutrient fermented liquid fertilizer in large scale by the CN201710082915.5 (China invention), the preparation method comprises the following steps: earthworm is taken as a source for extracting fermentation strains: culturing and separating at least one zymocyte to be detected from the earthworm material; screening of fermentation strains: screening fermentation bacteria to be detected by using the earthworm homogenate obtained by crushing the earthworms, and selecting strains capable of accelerating the fermentation speed to obtain at least one fermentation strain; and (3) large-scale fermentation: inoculating at least one fermentation strain into a fermentation tank containing the earthworm homogenate, and fermenting to obtain the earthworm fermentation liquid fertilizer.
Disclosure of Invention
The invention aims to provide a preparation method for producing a fresh earthworm total nutrient fermented liquid fertilizer in a large scale so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the preparation method for producing the fresh earthworm total nutrient fermented liquid fertilizer in large scale comprises the following steps:
s1: breeding earthworms to obtain living earthworms;
s2: extracting zymophyte from earthworm and screening zymophyte;
s3: putting the obtained zymophyte into a fermentation tank filled with leaves, oil residues, egg shells, earthworm primary pulp and water, and fermenting for 5 days, wherein the fermentation tank comprises the following materials in parts by weight: 60 parts of raw earthworm pulp, 4 parts of leaves, 5 parts of oil residue, 3 parts of egg shells, 50 parts of water and 11 parts of zymophyte;
s4: carrying out solid-liquid separation after fermentation is finished;
s5: collecting the liquid obtained in the step S4 to obtain the earthworm total nutrient fermented liquid fertilizer;
s6: and packaging the obtained earthworm total nutrient fermented liquid fertilizer.
Preferably, in S1, the earthworms to be cultivated are red earthworms.
Preferably, in S2, the earthworm homogenate obtained by crushing the earthworms is used to screen the fermentation bacteria to be tested, and the strain capable of accelerating the fermentation speed is selected, so as to obtain at least one fermentation strain.
Preferably, the steps of extracting the strain by taking the earthworms as a source are as follows: placing earthworm materials in sterile water, performing shake culture to obtain a mixed bacterial liquid, coating the mixed bacterial liquid on a solid flat plate for culture, selecting single bacterial colonies with obviously different culture characteristics, and scribing on the flat plate for purification to obtain at least one purified zymocyte to be detected; the screening steps of the fermentation strain are as follows: inoculating the earthworm homogenate cake into a PDA flat plate for culturing, inoculating fermentation bacteria to be tested at equal intervals around the earthworm homogenate cake, then placing the earthworm homogenate cake into an incubator for culturing again, and selecting strains capable of accelerating the fermentation speed to obtain at least one fermentation strain.
Preferably, in S3, the oil residue is oil residue of rape or peanut.
Preferably, in S3, the egg shells are washed prior to being placed in the fermentor.
Preferably, in S3, the leaves are rotten leaves.
Preferably, in S3, oxygen is introduced from the bottom of the tank during fermentation, the oxygen enters the fermentation tank in the form of uniform small bubbles, the top of the fermentation tank is filled with the oxygen to output waste gas, the pressure in the tank is kept at 0.1Pa, the temperature is 36 ℃, and the stirring speed is 160 r/min.
Preferably, in S4, the solid residue obtained after the solid-liquid separation is used as a solid earthworm fertilizer.
Compared with the prior art, the invention has the beneficial effects that:
1. the eggshells adopted by the fresh earthworm total nutrient fermented liquid fertilizer contain elements such as calcium, iron, zinc, magnesium, silicon and the like, and the fresh earthworm total nutrient fermented liquid fertilizer is applied to soil, can well improve the physical properties of the soil, enables the soil to be more loose, is air-permeable and water-draining, is beneficial to the growth of roots, is strong in roots, is luxuriant in flowers and leaves, is soft and good in air permeability, and is beneficial to the growth activities of microorganisms in the soil; the rotten leaves are also an organic fertilizer which contains a large number of elements required by plants, and also contains medium and trace elements required by the plants, and is non-toxic, pollution-free, root-burning-free and seedling-burning-free.
2. The raw materials adopted by the prepared fresh earthworm total nutrient fermented liquid fertilizer are all derived from common wastes in life, do not contain any agricultural chemicals, are convenient to take materials, and are low in cost, the prepared liquid fertilizer belongs to an organic fertilizer, the organic fertilizer has comprehensive nutrients, is rich in organic matters, humic acid and microbial flora, accelerates the propagation of microorganisms, is beneficial to the absorption of nutrients by plants, can decompose mineral substances in soil for the utilization of plants, is harmless to plants, people and livestock, can enhance the resistance of the plants to plant diseases and insect pests, inhibit the plant soil-borne diseases, improve the quality of crops, recover the natural flavor of the crops, and simultaneously avoid the pollution to the environment caused by the traditional compound fertilizer.
3. The leaves and the eggshells adopted by the preparation of the fresh earthworm total nutrient fermented liquid fertilizer belong to domestic garbage, and the invention recycles the same, thereby not only creating value again, but also lightening the burden of social treatment on the domestic garbage, and being beneficial to creating environment-friendly development and mutually-beneficial and win-win atmosphere.
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FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: the preparation method for producing the fresh earthworm total nutrient fermented liquid fertilizer in large scale comprises the following steps:
s1: breeding earthworms to obtain living earthworms, wherein the bred earthworms are red earthworms;
s2: extracting zymophyte from earthworms and screening zymophyte, screening zymophyte to be tested by using earthworm homogenate obtained by smashing earthworms, and selecting zymophyte capable of accelerating fermentation speed to obtain at least one zymophyte, wherein the step of extracting zymophyte from earthworms comprises the following steps: placing earthworm materials in sterile water, performing shake culture to obtain a mixed bacterial liquid, coating the mixed bacterial liquid on a solid flat plate for culture, selecting single bacterial colonies with obviously different culture characteristics, and scribing on the flat plate for purification to obtain at least one purified zymocyte to be detected; the screening steps of the fermentation strain are as follows: inoculating the earthworm homogenate cake into a PDA flat plate for culturing, inoculating fermentation bacteria to be tested at equal intervals around the earthworm homogenate cake, then putting the earthworm homogenate cake into an incubator for culturing again, and selecting strains capable of accelerating the fermentation speed to obtain at least one fermentation strain;
s3: putting the obtained zymophyte into a fermentation tank filled with leaves, oil residues, egg shells, earthworm primary pulp and water, and fermenting for 5 days, wherein the fermentation tank comprises the following materials in parts by weight: 60 parts of raw earthworm pulp, 4 parts of leaves, 5 parts of oil residue, 3 parts of egg shells, 50 parts of water and 11 parts of zymophyte, wherein the oil residue is residue after oil extraction of rape or peanut; cleaning eggshells before filling the eggshells into a fermentation tank; the leaves are rotten leaves; introducing oxygen from the bottom of the tank during fermentation, introducing the oxygen into the fermentation tank in the form of uniform small bubbles, filling the top of the fermentation tank to output waste gas, keeping the pressure in the tank at 0.1Pa, keeping the temperature at 36 ℃, and stirring at the speed of 160 r/min;
s4: after fermentation, carrying out solid-liquid separation, wherein solid residues obtained after solid-liquid separation can be used as solid earthworm fertilizers;
s5: collecting the liquid obtained in the step S4 to obtain the earthworm total nutrient fermented liquid fertilizer;
s6: and packaging the obtained earthworm total nutrient fermented liquid fertilizer.
The eggshells adopted by the fresh earthworm total nutrient fermented liquid fertilizer contain elements such as calcium, iron, zinc, magnesium, silicon and the like, and the fresh earthworm total nutrient fermented liquid fertilizer is applied to soil, can well improve the physical properties of the soil, enables the soil to be more loose, is air-permeable and water-draining, is beneficial to the growth of roots, is strong in roots, is luxuriant in flowers and leaves, is soft and good in air permeability, and is beneficial to the growth activities of microorganisms in the soil; the rotten leaves are also an organic fertilizer which contains a large amount of elements required by plants, medium and trace elements required by the plants, is non-toxic and pollution-free, and does not burn roots or seedlings; the raw materials adopted by the fresh earthworm total nutrient fermented liquid fertilizer are all derived from common wastes in life, do not contain any agricultural chemicals, are convenient to take materials, and are low in cost, the prepared liquid fertilizer belongs to an organic fertilizer, the organic fertilizer is comprehensive in nutrient, is rich in organic matters, humic acid and microbial flora, accelerates the propagation of microorganisms, is beneficial to the absorption of nutrients by plants, can decompose mineral substances in soil, is used by plants, is harmless to plants, people and livestock, can enhance the resistance of the plants to plant diseases and insect pests, inhibit plant soil-borne diseases, improve the quality of crops, recover the natural flavor of the crops, and simultaneously avoid the pollution of traditional compound fertilizers to the environment, wherein leaves and eggshells adopted in the raw materials belong to domestic garbage, the invention recycles the raw materials, not only creates value again, but also lightens the burden of the domestic garbage for social treatment, is beneficial to creating environment-friendly development and mutually-beneficial and win-win atmosphere.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents, and the invention is not limited to the embodiments described above, and various modifications and changes may be made without departing from the spirit and scope of the invention, and it is intended that all changes and modifications that fall within the scope of the invention are embraced in the appended claims.
Claims (9)
1. The preparation method for producing the fresh earthworm total nutrient fermented liquid fertilizer in large scale is characterized by comprising the following steps:
s1: breeding earthworms to obtain living earthworms;
s2: extracting zymophyte from earthworm and screening zymophyte;
s3: putting the obtained zymophyte into a fermentation tank filled with leaves, oil residues, egg shells, earthworm primary pulp and water, and fermenting for 5 days, wherein the fermentation tank comprises the following materials in parts by weight: 60 parts of raw earthworm pulp, 4 parts of leaves, 5 parts of oil residue, 3 parts of egg shells, 50 parts of water and 11 parts of zymophyte;
s4: carrying out solid-liquid separation after fermentation is finished;
s5: collecting the liquid obtained in the step S4 to obtain the earthworm total nutrient fermented liquid fertilizer;
s6: and packaging the obtained earthworm total nutrient fermented liquid fertilizer.
2. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S1, the earthworms to be cultivated are red earthworms.
3. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S2, screening the fermentation bacteria to be tested by using the earthworm homogenate obtained by crushing the earthworms, and selecting the strains capable of accelerating the fermentation speed to obtain at least one fermentation strain.
4. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 3, which is characterized by comprising the following steps: the steps of extracting the strain by taking the earthworms as the source specifically comprise: placing earthworm materials in sterile water, performing shake culture to obtain a mixed bacterial liquid, coating the mixed bacterial liquid on a solid flat plate for culture, selecting single bacterial colonies with obviously different culture characteristics, and scribing on the flat plate for purification to obtain at least one purified zymocyte to be detected; the screening steps of the fermentation strain are as follows: inoculating the earthworm homogenate cake into a PDA flat plate for culturing, inoculating fermentation bacteria to be tested at equal intervals around the earthworm homogenate cake, then placing the earthworm homogenate cake into an incubator for culturing again, and selecting strains capable of accelerating the fermentation speed to obtain at least one fermentation strain.
5. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S3, the oil residue is oil residue of rape or peanut.
6. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S3, the egg shells are washed before being placed in the fermenter.
7. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S3, the leaves are rotten leaves.
8. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S3, oxygen is introduced from the bottom of the tank during fermentation, the oxygen enters the fermentation tank in the form of uniform small bubbles, the top of the fermentation tank is filled with the oxygen to output waste gas, the pressure in the tank is kept at 0.1Pa, the temperature is 36 ℃, and the stirring speed is 160 r/min.
9. The preparation method of the full-nutrient fermented liquid fertilizer for the fresh earthworms in large-scale production according to claim 1, which is characterized by comprising the following steps: in S4, the solid residue obtained after the solid-liquid separation can be used as a solid earthworm fertilizer.
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| CN202010014868.2A CN111763127A (en) | 2020-01-07 | 2020-01-07 | Preparation method for producing fresh earthworm full-nutrient fermented liquid fertilizer in large scale |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113024319A (en) * | 2021-03-26 | 2021-06-25 | 湖南省林业科学院 | Foliar fertilizer and preparation method and application thereof |
| CN115772511A (en) * | 2022-12-22 | 2023-03-10 | 南开大学 | Method for efficiently extracting full resources from lumbrokinase |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20170039857A (en) * | 2015-10-02 | 2017-04-12 | (주)삼사라 | Liquid fertilizer and manufacturing method thereof |
| CN106810399A (en) * | 2017-02-16 | 2017-06-09 | 宁夏大学 | The preparation method of the full nutritious fermentation liquor body fertilizer of the fresh earthworm of large-scale production |
| CN107285962A (en) * | 2017-08-02 | 2017-10-24 | 天津天丰泽田生物科技有限公司 | A kind of Water soluble fertilizer containing earth-worm extractive as raw material |
| CN110436984A (en) * | 2019-09-17 | 2019-11-12 | 廖威 | A kind of production method of earthworm foliar fertilizer |
-
2020
- 2020-01-07 CN CN202010014868.2A patent/CN111763127A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170039857A (en) * | 2015-10-02 | 2017-04-12 | (주)삼사라 | Liquid fertilizer and manufacturing method thereof |
| CN106810399A (en) * | 2017-02-16 | 2017-06-09 | 宁夏大学 | The preparation method of the full nutritious fermentation liquor body fertilizer of the fresh earthworm of large-scale production |
| CN107285962A (en) * | 2017-08-02 | 2017-10-24 | 天津天丰泽田生物科技有限公司 | A kind of Water soluble fertilizer containing earth-worm extractive as raw material |
| CN110436984A (en) * | 2019-09-17 | 2019-11-12 | 廖威 | A kind of production method of earthworm foliar fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113024319A (en) * | 2021-03-26 | 2021-06-25 | 湖南省林业科学院 | Foliar fertilizer and preparation method and application thereof |
| CN115772511A (en) * | 2022-12-22 | 2023-03-10 | 南开大学 | Method for efficiently extracting full resources from lumbrokinase |
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