CN111743934A

CN111743934A - Traditional Chinese medicine composition for regulating intestinal flora and application thereof

Info

Publication number: CN111743934A
Application number: CN202010581864.2A
Authority: CN
Inventors: 李昀泽; 陈红; 李建军; 常诚; 姚阳婧; 陈安琪; 顾锡镇; 裔楠; 常荧荥; 曹欣然
Original assignee: Jiangsu Provincial Hospital of Chinese Medicine
Current assignee: Jiangsu Provincial Hospital of Chinese Medicine
Priority date: 2020-06-23
Filing date: 2020-06-23
Publication date: 2020-10-09

Abstract

The invention discloses a traditional Chinese medicine composition for regulating intestinal flora, which is characterized by comprising the following components in parts by weight: 10-15 parts of silkworm excrement, 6-9 parts of tabasheer, 4.5-9 parts of trogopterus dung and 3-6 parts of cassiae toralis. The invention further provides an application of the traditional Chinese medicine composition in preparing a medicine for improving the relevant diseases of the vascular cognitive disorder, wherein the relevant diseases of the vascular cognitive disorder are any one of non-dementia type vascular cognitive disorder, vascular cognitive disorder combined sleep disorder, adrenoleukodystrophy, acute ischemic-hypoxic encephalopathy, intestinal flora shift encephalopathy, acute ischemic stroke and acute central retinal artery occlusion. The traditional Chinese medicine composition disclosed by the invention is simple in formula and wide in source, can effectively improve vascular cognitive dysfunction, and has a certain intervention effect on various accompanying risk factors such as hypertension, hypercholesterolemia, diabetes, metabolic syndrome, atherosclerosis and the like.

Description

Traditional Chinese medicine composition for regulating intestinal flora and application thereof

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a pharmaceutical composition for improving vascular cognitive impairment.

Background

Vascular cognitive impairment is a syndrome of acquired elevation and impaired cognitive function, a syndrome that ranges from mild cognitive impairment to dementia caused by cerebrovascular disease-related risk factors, overt or non-overt cerebrovascular disease. Among them, the cerebrovascular risk factors include hypertension, diabetes, hyperlipidemia, etc. The vascular cognitive disorder comprises non-dementia vascular cognitive disorder, vascular dementia and senile dementia accompanied with vascular factors, namely mixed dementia. Non-dementia-type vascular cognitive disorders refer to early-stage vascular cognitive disorders that have not yet met the criteria for dementia. Research has found that to date, the only cognitive disorders that can be prevented are vascular cognitive disorders. If effective intervention is available at the beginning of the disease, when the patient's daily life has not been affected, continued deterioration of cognitive ability can be avoided, preventing its progression to the irreversible vascular dementia stage.

Patients with vascular cognitive impairment mainly manifest neuropsychological abnormalities, and the most prominent ones manifest themselves as impaired executive function accompanied by memory loss, and possibly other cognitive dysfunctions such as attention, abstract thinking, concept formation and conversion, and information processing speed. Memory impairment is manifested as selective macular degeneration, and psychomotor disturbances are directly related to cerebrovascular lesions and brain tissue location and volume. Cognitive dysfunction is manifested by reduced recent memory and computing power, slower information processing speed, decreased analysis, summarization and judgment, language disinclination, inability to perform familiar work and normal communication, getting lost when going out, inability to recognize family, clothing mistake, incontinence of urine and stool, and the like, and finally, life cannot be taken care of by oneself. In addition, patients may be associated with apathy, mood swings, depression, anxiety and other mental symptoms.

At present, clinical manifestations, neurological assessments and neuroimaging examinations are the main means of determining vascular cognitive disorders. The patient should first present symptoms of cognitive impairment, meet the diagnostic criteria for cognitive dysfunction, and should also be accompanied by cerebrovascular disease, and it must be excluded that the cognitive impairment which occurs is caused by non-vascular factors. Expert consensus on the impairment of vascular cognitive function states that vascular cognitive disorders represent conditions of different etiology, pathogenesis, pathological changes and clinical manifestations. The diagnostic criteria for non-dementia type vascular cognitive disorders are: not meeting the dementia standard; cognitive impairment is considered to be vascular and has evidence of sudden onset, a stepped course and macular cognitive impairment; there is evidence of atherosclerosis, focal signs and imaging evidence; vascular risk factors, but not vascular risk factors alone and without infarction.

The modern medicine does not find specific medicines for vascular cognitive impairment, and the currently available medicines all improve the microenvironment of brain tissues from different aspects, provide good internal environment for the recovery of cerebral neurons and promote the recovery of diseases.

First-aid and western-medicine treatment method

(1) Regulating blood pressure

Hypertension is a high risk factor for cerebrovascular diseases and plays an important role in cognitive impairment. The scholars find that the hypertension aggravates the degree of atherosclerosis of large and medium sizes, destroys the self-regulation function of cerebral vessels, and denaturalizes the wall glass of arterioles, so that the white matter of the brain is diseased. White matter lesions in the brain, especially the paraventricular white matter regions, are closely associated with cognitive function. The increase of systolic pressure or diastolic pressure can cause cognitive function impairment, and the maintenance of proper blood pressure can prevent and delay the occurrence of vascular cognitive disorder.

(2) Improving cerebral circulation

Dihydro ergot alkalines. The medicine can eliminate vasospasm, increase cerebral blood flow, and improve the utilization function of neurons, and has wide clinical application, such as commonly used nicergoline, bimagroline, etc. ② calcium antagonist. These herbs are mainly used to dilate cerebral blood vessels, increase cerebral blood flow, and prevent calcium overload and free radical damage. ③ nicotinic acid. Dilate blood vessels, increase cerebral blood flow and improve memory.

(3) Anti-platelet aggregation

The platelet aggregation resisting medicine has the main functions of inhibiting platelet aggregation and stabilizing platelet membrane, so as to improve cerebral blood circulation and prevent intravascular thrombosis. Such as: aspirin, clopidogrel, and the like.

(4) Brain cell metabolism improving agent

The medicine promotes brain cells to utilize glucose, phospholipid and amino acid, so as to enhance the reactivity and excitability of patients and enhance the learning and memory. Aniracetam, piracetam such as the exemestane class; meclofen vinegar; amitrazine; a brain-active agent; citicoline; ATP; coenzyme A, and the like.

(5) Brain protective agent

Such as flunarizine, magnesium sulfate, ginkgo leaves, etc.

(6) Choline acetase inhibitor

Such as donepezil and the like.

(7) Rehabilitation therapy

The rehabilitation therapy comprises daily life capacity training, muscle and joint activity degree training, speech disorder rehabilitation and the like.

Second, Chinese traditional treatment method

In the concept of traditional Chinese medicine, the vascular cognitive disorder is considered as 'dull disease', 'dementia', 'amnesia', etc. The symptoms of the disease have been described in "Zuo Shi Chun & Cheng Gong eighteen years" of spring and autumn at the earliest time: "Zhouzi, with brother but without coma, can not distinguish Shu Mai, so it is not immediately available". Zhang Jingyue is recorded in Jingyue quan Shu: the disease is marked by the special theory of dementia for the first time and is regarded as an independent disease. Qing, Chenshize bell, in the book of syndrome differentiation: if people are old and forgetful, they are at a glance and do not remember, but are just at a glance despite the fact that people are at a perplexity, which is the extreme of true forgetfulness, the first cause of dull disease.

With the intensive research on the dull disease, the dull disease is found to occur not only in the elderly but also after stroke, as described in clinical guideline medical records by phyllosphaer: 'apoplexy at the beginning, loss of spirit and enuresis, obvious in the faint of the old'. Shenjinao Xi (source of miscellaneous diseases xi Zhu) also has the action of inducing forgetfulness after stroke. For the patient with marrow capping, kidney essence is generated, essence is sufficient to make marrow foot, marrow is in bone, marrow foot is strong, so it can strengthen the body and strengthen the body. Essence is generated by the spirit, and essence is sufficient and strong, so the people have many skills. The people with insufficient marrow have weak strength, the people with insufficient essence have little intelligence, the kidney essence is sufficient, the marrow sea is nourished, and the brain is full, so that the ears, eyes, intelligence and vigor can be obtained, the intelligence is sensitive, and the activities are quick. Cloud of the book of Yi Tong Jing Yi of traditional Chinese and Western medicine: "when the mind is not remembered, it is in the kidney essence, tonifying the kidney and producing essence, which is stored in the brain". Essential qi in kidney is the innate root, kidney yin and kidney yang is the root for yin and yang growth of each zang-fu organ, and it can tonify kidney yin and kidney yang, and yin and yang growth of each zang-fu organ is active, so it can perform normal function, and brain can nourish kidney essence, so it can clear mind. Professor Yao Baofan appreciates that' treating anorexia must tonify kidney, and kidney essence is sufficient to nourish marrow; the professor of old people can tonify the original qi and improve the brain by self, tonify the kidney, eliminate phlegm and remove blood stasis to treat dementia, and has obvious curative effect.

The patients with vascular cognitive impairment are old, the spleen and stomach function is degraded, the patients are disadvantaged in ascending and descending, tired in healthy transportation, and the food essence cannot be normally distributed. Failure of the spleen to transport and transform is the source of fatigue, accumulation of dampness leading to fatigue and fluid retention, obstruction of middle energizer, mistaking of clear resources and mistaking of mind. Patients often have the symptoms of nausea, high disease, dizziness, thought insensitivity and the like. Chenshize bell has been recorded in Bian Bing Lu (records of syndrome differentiation): the theory of treating stagnation without strange measures and treating stagnation with phlegm, which defines the position of the phlegm-resolving method in treating the dull disease and provides the main ideas of resolving stagnation, expelling phlegm, invigorating stomach and ventilating. 'Yi Lin rope ink' is recorded in the legend: qin Lian Er Chen Tang is indicated for the syndrome of phlegm retention due to its insitu, forgetfulness and unknown constitution.

Liu xing Wang treats vascular cognitive disorder from phlegm and blood stasis, modified from Daqu phlegm decoction, and modified from medicinal calcined chlorite schist, fructus trichosanthis, Salvia miltiorrhiza, radix pseudostellariae, earthworm, ginger processed pinellia tuber, radix curcumae, Poria cocos, rhizoma acori graminei, radix paeoniae rubrathe, leech, rhizoma cyperi, arisaema cum bile and Tabanus, and the effective rate reaches 86.2% after 1-4 treatment courses. The basic pathogenesis of the vascular cognitive disorder is kidney deficiency and stagnation of turbid phlegm and blood stasis by professors of Zhengshao provinces of famous and old Chinese medicine in Henan, and emphasizes that the patients with the vascular cognitive disorder are properly treated with kidney tonifying products for reinforcing vitality regardless of the existence of obvious kidney deficiency. The prepared Chinese patent medicine brain-strengthening intelligence-recovering oral liquid for tonifying kidney, benefiting marrow, increasing intelligence, promoting blood circulation to remove blood stasis can obviously improve the living behavior ability and cognitive level of patients and improve the condition of nerve defect.

At present, no guide is recommended for modern medical medication, only experts agree that the clinical symptoms can be partially improved, and strong clinical evidence does not exist.

The traditional Chinese medicine culture is a decay civilization which is inspected for thousands of years, is thought pearl which is handed down by ancestors in ancestry, and in a plurality of methods for treating the vascular cognitive impairment by the traditional Chinese medicine, the curative effects of various treatment methods such as kidney tonifying, phlegm reducing, blood stasis removing, qi activity regulating and bowel relaxing and turbidity removing treatment methods are confirmed, but the treatment methods are limited for a long time, are not further innovated, and are not changed according to changes of life styles and the like of people of new generations.

The applicant has found through recent studies on the intestinal flora in vascular cognitive disorders that the intestinal flora can influence neurobehavioral disorders through the gut-brain axis. The normal intestinal flora plays an important role in maintaining intestinal homeostasis. The intestinal flora contains 1013-1014 bacterial bodies, the number of the bacterial bodies is 10 times more than that of human somatic cells, the number of the bacterial bodies included in the intestinal flora is more than 100 times of that of human genome genes, and the intestinal flora has been widely accepted in aspects of preventing pathogen invasion, synthesizing vitamins, metabolizing substances, resisting cancers and the like. In 1958, Eiseman et al first reported the use of fecal bacteria transplantation for the treatment of membranous enteritis in humans. Fecal bacteria were transplanted in 1958 for the treatment of refractory and recurrent clostridium difficile infections. Recent studies have shown that fecal transplantation is an effective treatment for refractory and recurrent clostridium difficile infections with success rates in excess of 90% and can be considered as an antibiotic alternative. However, no effective clinical studies have been made for the treatment of cognitive impairment by intestinal flora transplantation.

In addition, the current fecal bacteria transplantation treatment is difficult to develop, and has higher requirements on selection of a fecal donor, preparation of fecal bacteria liquid and preparation of a receptor before transplantation. The excrement generally selects healthy offspring of patients or other healthy family members as excrement donors, and compared with blood donation, the excrement donor has higher requirements, and the excrement contains more infectious microbes and viruses, so that the risk factor is increased for implementation. Fecal donor inclusion criteria included: antibiotics, proton pump inhibitors and other drugs which may affect the intestinal flora of the donor are not taken in nearly three months. ② there is no history of infectious diseases such as hepatitis B, hepatitis C, AIDS, syphilis, tuberculosis, etc. And thirdly, the digestive system symptoms and diseases such as constipation, colonitis, gastrointestinal tumor and the like are avoided. Fourthly, the disease history of the immune system is not existed. (iv) recent travel history in non-epidemic diarrhea areas. Sixthly, there is no history of drug absorption and swimming aid. The common approaches of fecal bacteria transplantation include oral administration, nasogastric tube, nasojejunal tube, gastroduodenal, enteroscope, retention enema, fistulization opening and the like, and the fecal bacteria transplantation effects through different approaches are different. Most patients have difficulty accepting the above routes. The common adverse reactions of the coprophilous fungi transplantation mainly comprise abdominal distension, diarrhea, constipation, fever and the like, and other adverse behaviors are mostly related to the coprophilous fungi transplantation way.

The long history of intervention of traditional Chinese medicine fecal bacteria transplantation on vascular cognitive impairment is seen in Su Wen & Abzhong Lun of Huangdi's Canon, and discussion on treatment of distension with carignane and animal feces is recorded. The golden juice in Kudzuvine flood 'elbow reserve fast prescription' in Dongjin period is taken orally, and the excrement juice of healthy people is used for treating food poisoning and severe diarrhea. Li Shizhen in the Ming Dynasty of China recorded in the compendium of herbage that the human suffering from diarrhea, fever, vomiting and constipation can take the excrement of healthy people to achieve the purpose of treatment. The leaf Tianshi of one of the four kingdoms of Jinyuan, in the "tepid treatise," it is indicated that "the herb with jin juice can be added if the ying system is heated … … and restlessness and constipation are added. In the Ming and Qing dynasty, along with the perfection of the theory of the science of epidemic febrile disease and the continuous deepening of clinical practice, the traditional Chinese medicine gold juice has more and more extensive clinical application and often obtains unexpected treatment effect. The intestinal flora is a "physiological organ" essential for the survival of the host. Various risk factors of vascular cognitive disorders such as hypertension, hypercholesterolemia, diabetes, metabolic syndrome, the onset of atherosclerosis, etc., in which the intestinal flora plays a non-negligible role.

In the literature, we found that, since animal and human feces have medical records for oral treatment of diseases in ancient times, and the feces of healthy human have insufficient supply, why we did not select suitable animal feces for intestinal flora regulation? Selecting animal or human feces, excrement and other fermentation products to be added into Chinese medicinal varieties, screening certain Chinese medicaments in the Chinese medicinal varieties through experiments, wherein the structure of intestinal flora can be directly adjusted, and the micro-ecological environment can be improved, and establishing the Chinese medicinal intestinal flora regulating formula to ensure that normal flora can better survive and grow, so as to improve vascular cognitive impairment; or the intestinal flora influences the absorption, metabolism, transformation and the like of other oral traditional Chinese medicines in vivo, and synergistically promotes other traditional Chinese medicines to exert curative effects.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to provide a pharmaceutical composition for improving vascular cognitive impairment, which improves the micro-ecological environment by directly adjusting the intestinal flora structure, so that normal flora can better survive and grow, and further improves the vascular cognitive impairment, or enables the intestinal flora to influence the absorption, metabolism, conversion and the like of other oral traditional Chinese medicines in vivo, and synergistically promotes other traditional Chinese medicines to exert curative effects.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a traditional Chinese medicine composition for regulating intestinal flora comprises the following components in parts by weight: 10-15 parts of silkworm excrement, 6-9 parts of tabasheer, 4.5-9 parts of trogopterus dung and 3-6 parts of cassiae toralis.

Preferably, the pharmaceutical composition comprises the following components in parts by weight: 10-15 parts of silkworm excrement, 6-9 parts of rhus chinensis, 4.5-9 parts of trogopterus dung, 3-6 parts of cassiae streptacanthae, 5-10 parts of human urine and 3 parts of cordyceps militaris.

The invention further provides application of the traditional Chinese medicine composition in preparing a medicine for improving the relevant symptoms of the vascular cognitive impairment.

The disease related to the vascular cognitive disorder is any one of non-dementia vascular cognitive disorder, vascular cognitive disorder combined with sleep disorder, adrenoleukodystrophy, acute ischemic-hypoxic encephalopathy, intestinal flora shift encephalopathy, acute ischemic stroke and acute retinal central artery occlusion.

Specifically, when preparing the medicament for improving the adrenoleukodystrophy, the Chinese medicinal composition for tonifying qi, activating blood and nourishing blood and the Chinese medicinal composition for regulating the intestinal flora are used in combination.

The traditional Chinese medicine composition for tonifying qi, promoting blood circulation and nourishing blood comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus, 6-10 parts of cassia twig, 15-30 parts of fried white peony root, 6-10 parts of medicinal cyathula root, 6-10 parts of radix achyranthis bidentatae and 6-10 parts of eucommia ulmoides; the dosage ratio of the traditional Chinese medicine composition for tonifying qi, promoting blood circulation and nourishing blood to the traditional Chinese medicine composition for regulating intestinal flora is 1: 1.

When preparing the medicine for improving the intestinal flora shift encephalopathy, the traditional Chinese medicine composition for clearing lung and eliminating phlegm and the intestinal flora regulating traditional Chinese medicine composition are used in a combined manner.

The lung clearing and phlegm eliminating traditional Chinese medicine composition comprises the following components in parts by weight: 6-10 parts of cortex mori, 3-6g parts of platycodon grandiflorum, 6-10 parts of flos farfarae, 15-30 parts of gypsum, 6-10 parts of scutellaria baicalensis, 6-10 parts of prepared rhubarb and 10-15 parts of radix rehmanniae; the dosage ratio of the lung clearing and phlegm eliminating traditional Chinese medicine composition to the intestinal flora regulating traditional Chinese medicine composition is 1: 1.

The traditional Chinese medicine composition for regulating intestinal flora can be prepared into any one of water decoction, granules, tablets, capsules, paste and wine. The preparation method can be used for preparing the compound according to the conventional preparation method.

The silkworm excrement is dry excrement of 2-3 dormant larvae of bombyx mori which is an insect in the family Bombycidae, and is prepared by processing raw medicinal materials, removing impurities, and screening to remove ash and scraps, wherein the silkworm excrement is short cylindrical particles with the characteristics of 0.3cm length, black brown surface, roughness and unevenness, and is provided with 6 longitudinal grooves, the top surface is hexagonal, and two ends are flat, crisp, fragile and slightly smelly.

The homo is prepared by soaking Glycyrrhrizae radix powder in urine of human body in bamboo tube. Processing comprises sawing off herba Lophatheri, removing surf, placing Glycyrrhrizae radix coarse powder in bamboo tube, pressing, plugging with paper and cloth, melting with Colophonium, sealing, hanging in human urine jar for 2-3 months, soaking in water before winter, taking out in spring, and rinsing in clear water for 1 month. Changing water for 1 time every day, taking out, drying in the shade, splitting the bamboo tube, and taking out the yellow wine. Sun-drying until no odor exists, sun-drying, and cutting into segments or blocks. The shape is cylindrical section or irregular block. Dark yellow, coarse and dense, and the licorice fibers are seen to be criss-cross interwoven. Compact in texture and has a special smell.

Trogopterus dung is dry feces of compound-tooth squirrel of the family Trogopterus. Processing to obtain raw materials, removing impurities, sieving to remove dust, and knocking into small pieces when the pieces are large. The shape of the granules is irregular blocky or oblong granules. Brown or gray brown surface, oily luster, hardness, yellowish brown cross section, unevenness and fibrous nature. Fishy smell.

The Wanyuesha is dry feces of hare such as south China rabbit of Lagotidae. Processing to obtain raw materials, and removing impurities. The straw-like food has the characteristics of elliptic spherical particles, different sizes, rough surface, light yellow straw fiber clamped in the straw-like food, gray yellow or gray brown, light and fragile quality, herbaceous appearance after being crushed and slight smell.

Human urine is a solid substance deposited in a toilet bowl by the urine of a human family. Processing to obtain raw medicinal materials, removing impurities, rinsing for 4-7 days, changing water frequently, taking out, sun-drying overnight dew for about 15 days, turning over for 1 time every day until no odor exists, and sun-drying. The characters are blocks with different sizes, light gray, gray white or light gray yellow, hard and crisp, and the section is in layered texture with different densities. Without urine and smell.

The Cordyceps militaris is a dry complex of a stroma and a pupa body cultured by inoculating the Cordyceps militaris of the Clavipitaceae family onto live pupa of tussah silkworm of the Tectalidaceae family for about 45 days at 15-22 ℃. Processing to obtain raw materials, removing impurities, and drying at low temperature. The character is formed by connecting a pupa body with a plurality of sub-seats growing from the pupa body, and the pupa body and the sub-seats are tightly combined. The pupa body is oblong, 2-4.5 cm long and 1-2.5 cm in diameter, yellow to yellow mycoderm covers the surface of the pupa body, the pupa body is black brown to black after being peeled off, and 12-25 annular grains are formed in the abdomen of the pupa body; brittle and easy to break, and has a slightly flat cross section and a grayish brown color. 4-20 stroma, slender flat cylinder, length of 2-8 cm, diameter of about 0.2cm, surface orange yellow to pink, few branches, occasionally expanded top; soft texture, yellow-white cross section, slight fishy smell, and slightly salty taste.

Silkworm excrement is sweet and pungent, warm and enters liver and spleen meridians. Has the functions of dispelling wind, eliminating dampness, promoting blood circulation and relieving pain. It is used to treat rheumatic arthralgia, rubella, headache, arthralgia, acute vomiting, diarrhea, cold pain of waist and foot, and rheumatic eye disease. The book of entry records that it can enter intestine: "dominating borborborygmus, heat in the middle energizer, diabetes, wind arthralgia, urticaria". The record of the treatment of apoplexy (cerebrovascular disease) with this herb entering the intestine is recorded in Ben Cao Shi Yi (A supplement to the materia Medica): parching to yellow, soaking in wine in bags, dispelling pathogenic wind, relieving symptoms, treating arthralgia, cold in abdomen, blood stasis, lumbago and skelalgia; stir-baked to heat, bag-filled to warm and iron, mainly due to wind, paralysis of tendons and bones, involuntary movement of hands and feet, and soreness of waist and feet, persistent arthralgia of skin. The compendium of materia Medica also has records: for treating diabetes, symptom knot, metrorrhagia, head wind, red eyes, wind and dampness removal of women, the clinical proved recipe is summarized as follows: treating hemiplegia: silkworm excrement is two major. Steaming the two bags, and applying to the affected part. The lamb tripe and the polished round-grained rice are still used for cooking the porridge, one porridge is eaten in one day, and the porridge is stopped for ten days. The 'Quanzhou materia medica' records the effective action of the product in treating encephalopathy: "treating wind-cold type common cold and migraine", and summarizing compound recipe: for treating headache due to exogenous pathogens: silkworm dropping, dahurian angelica root and rhubarb. Grind them into fine powder, mix with scallion soup and apply externally.

Huang and gan, salty and cold in nature enter stomach meridian. Has effects in clearing away heat, cooling blood, and removing toxic materials. It is indicated for typhoid fever, polydipsia, heat-toxin macula, erysipelas, and sores and ulcers. The book Ben Cao Jing Shu has records: "relieving stomach heat toxin (modern stomach, small intestine, large intestine). The materia Medica is in the future: purging heat, clearing phlegm-fire, resolving food stagnation, and relieving excess heat of five zang organs. It is indicated for mania due to heat in the daytime, acne with blood heat and black body. Various documents record that the product can regulate intestinal flora and treat intestinal flora shift: the records of materia medica Zhenzhuang recorded in the book of materia medica Zhenzhen, the book-carried work is specially designed for stomach detoxification, and the taste is sweet. It can relieve excess heat of five zang organs, and is also indicated for the reason of its cold in qi, in Ben Cao Meng quan (materia Medica curtain for treating epidemic toxicity), and in Ben Jing Yuan (materia Medica) for relieving the weather, dispelling heat and warm toxicity and generating macula. The "Songfeng Bian Yan" (pine peak epidemic diseases) has a "Renzhong pill": there are no restrictions on the amount of yellow in the middle, and the rice is in the form of pills, while mung bean is large and fifteen pills are added. Recorded for the first time in Danxi Xin Fa, the medicine is used for treating neuropsychological diseases, namely patients with vexation and steaming bone, yellow in the middle of the human body is taken as powder, three pieces of money are taken each time, and the juice of radix Rubiae, succus Bambusae and succus Zingiberis recens are taken uniformly.

Wulingzhi is sweet and warm. It has effects in promoting blood circulation, dispelling blood stasis, and stopping bleeding. The compendium of materia Medica records: wulingzhi, the liver meridian of foot jueyin, also has a strong smell and yin in yin, so it enters blood system. Liver governs blood, so this herb can cure blood disease, disperse blood and stop pain. It can also be used in liver meridian to relieve convulsions, remove malaria and dysentery, and resolve phlegm. The 'Laiyouji Yangfang' has Zijin pills: treating headache and anorexia; the trogopterus dung is washed with water, 100g of fried powder is diluted by rice vinegar, and the fried powder is decocted into paste by slow fire, added with the real cattail pollen and pills like longan. One pill is taken every time, half of the urinate of boys is decocted to seven minutes, and the pill is taken warmly and again after a few hectares; for amenorrhea, it is taken after being ground with wine. Lingzhi Wan from Yang Jia Zang Fang (Yang's prescription for storage) can be used for treating all the pain of heart and abdomen and qi of small intestine. Iron pills in Sheng Ji Zong Lu (holy Ji Zong Lu) are indicated for wind bias and apoplexy. Comprises the following steps: oletum Trogopterori, and radix Aconiti Kusnezoffii (peeled, umbilicus and sharp, and used unprocessed, and each is taken to be clean powder) by equal parts. Mixing and grinding, adding fresh water, and placing into mortar with pestle for several times, and making pill with big marble, and placing in the silk bag. Each pill is taken four times, after being chewed and drunk with warm wine and taken ten times, one pill is taken two times; after more ten days, one pill should be taken for four times, which is worse in one month. If the wine is not eaten, the mint tea can be eaten as well, but the mint tea cannot be drunk as the wine. Recorded in the empirical prescription of "Wuxiang pill", the product can enter intestines and has the functions of promoting digestion, removing food retention, relieving stuffiness, eliminating phlegm, eliminating qi, eliminating stagnation, reducing swelling, relieving pain, eliminating blood, relieving dysentery, eliminating handless cup, eliminating diaphragm, swelling and stuffiness, and treating stagnation of phlegm, mental confusion, and the like. For the translocation of intestinal flora, the 'Shumei pill' is available from 'Puji Fang': oletum Trogopterori, pollen Typhae (parched), and Moschus. The powder is added, and the refined honey and the pills are as big as phoenix tree seeds. One pill is taken every time, and the decoction is added with vinegar.

Yueyuesha is pungent in flavor and cold in nature, entering liver and lung meridians. The record of Yaojiantan Ji Yan Fang records that the product: it can remove nebula, improve vision, detoxify and kill parasites. Treating hemorrhoids with continuous bleeding and pain: perform a yellow color with slow fire, add Olibanum and Olibanum half money every two money, and take three or four times a day with hollow warm wine. The herbal Meng quan (Chinese materia Medica) has: "treating acne and producing eye sore, hemorrhoid, intestine and head bleeding". The compendium of materia medica considers that the product can regulate the intestinal flora: for tuberculosis, cough, anal fistula, eczema and fistula, it can kill parasites and remove toxicity. The "materia medica seek truth" also includes: rabbit feces can improve eyesight to remove nebula, and symptoms such as tuberculous, and pungent and hard to dissipate stagnation, so it can be taken actively, if the vital energy of.

White in the middle of the human body, salty in taste and cool in nature. It enters lung, heart and bladder meridians. Has the functions of clearing heat, reducing pathogenic fire, stopping bleeding and removing blood stasis. The record of 'one drop of gold' in 'Puji Fang' can be used for treating migraine and headache: white in human body, earthworm (stir-fried) and equal parts. The sheep bile and the pill mustard are big in powder. The new pill is pumped into the nose

It is said. The compendium of materia Medica is as follows: the recipe is also indicated for the reason that people are white in middle energizer, and have the actions of lowering ministerial fire, eliminating blood stasis, and salty in cover can moisten and remove blood. It is effective in treating various sores on the mouth and tongue, and can be used for the treatment of fire-purging. Bencao Jing Shu (Bencao Jing Shu) believes that it can be used for treating excessive fire in intestinal tract, homo, which is salty in taste, cool in smell, non-toxic, and can purge excessive fire from liver, kidney, triple energizer, and bladder. For epistaxis, it can enter all meridians to purge fire and eliminate pathogenic factors, and for diseases caused by overstrain heat, consumptive lung disease, heart and diaphragm heat, hematemesis, emaciation and thirst, it is also recorded in Benjing. Cold can remove heat, so it is indicated for sores due to fire. It is indicated for aphtha and malnutrition of the mouth and tongue. The herbal essentials records that it can regulate intestinal function: the recipe of Maren donkey-hide gelatin for treating blood deficiency and constipation, the recipe of Ji Yu for treating spider bite, yang deficiency without fire, indigestion and intestine without excess, is also named as drowning and whiting. The book materia medica easy reading includes: it is indicated for consumptive lung disease due to heat-evil, and sores due to pathogenic fire and dampness. Sores in the mouth, tongue and throat, and infantile malnutrition of the eyes and orifices. There is also a record of this herb for treating intestine in De Cang Ben Cao: the fried earthworm powder and sheep bile nose are used for treating migraine and headache. It is combined with Ji Yun to treat spider venom. It is combined with Ma ren and E jiao to treat constipation. And dried alum, mixed with mouth sores. Combined with Aerugo and musk, it is indicated for malnutrition of horse.

Cordyceps militaris is sweet and neutral in nature and enters lung and kidney meridians. Has the functions of tonifying lung and kidney, stopping bleeding and reducing phlegm. It is considered from the medical examination that it can nourish qi and tonify life. Meanwhile, the Chinese medicinal preparation also has a treatment effect on gastrointestinal diseases, and the Chongqingtang pen has the following effects: cordyceps sinensis, a holy drug with mild and mild tonifying property, is a holy drug for malaria, distension and pain, and has the effect of treating aspongopus. Zhao Zhimin (materia Medica supplement from Yi Xue) records the gastrointestinal tract regulating action of the product: "Panyouxinyun treats diaphragm disease, Zhou Fang Shi Yun treats parasitic tympanites", and simultaneously "Xia Cao Dong, which has the same action as ren Shen, can treat all kinds of deficiency and loss. So it can obtain the balance of yin and yang qi. It has the same action as ren Shen and Rong, but it is mild in property and suitable for both old and young patients, so it is considered to have action on both old and young deficiency diseases. The Tibetan medicine considers that the medicine is mainly used for kidney and waist pain, impotence, spermatorrhea, weakness and old injury.

At present, no combination of the medicines exists in patent medicine, and only the children zhuang powder contains human middle yellow and human middle white. Through literature search, we find that all the traditional Chinese medicines of the past generation are widely applied and are still commonly used in modern clinic, and the application range of the traditional Chinese medicines is wide, and the traditional Chinese medicines cover different medicinal properties, different medicinal flavors and different attributive meridians. Experiments and clinical curative effect verification show that the traditional Chinese medicine intestinal flora regulating prescription belongs to the optimal combination selected from a plurality of traditional Chinese medicines for regulating intestinal flora, and no adverse reaction occurs in an experimental model and a patient using prescription, which is incomparable to the single accumulation of excrement traditional Chinese medicines. And because the taste is good, the acceptance degree of the traditional Chinese medicine is high for patients, and a brand new situation is opened up by scientific research on the traditional Chinese medicine for regulating the intestinal flora in China.

Has the advantages that: the traditional Chinese medicine composition disclosed by the invention is simple in formula and wide in source, can effectively improve vascular cognitive dysfunction, and has a certain intervention effect on various accompanying risk factors such as hypertension, hypercholesterolemia, diabetes, metabolic syndrome, atherosclerosis and the like.

Detailed Description

The present invention will be described in detail below with reference to specific examples. The traditional Chinese medicine in the following embodiments is prepared by the following preparation method:

the silkworm excrement is dry excrement of 2-3 dormant larvae of bombyx mori which is an insect in the family Bombycidae, and is prepared by processing raw medicinal materials, removing impurities, and screening to remove ash and scraps, wherein the silkworm excrement is short cylindrical particles with a shape of 0.3cm, a black brown surface, a rough surface, uneven surfaces, 6 longitudinal grooves, a hexagonal top surface, two flat ends, crisp texture, fragility and slight odor.

Example 1 effective drug screening of a traditional Chinese medicine intestinal flora regulating prescription and therapeutic effect observation of a rat cerebral ischemia and hypoxia model.

The damage of cognitive function is one of the more obvious clinical manifestations of cerebral ischemia and hypoxia patients, particularly the vascular cognitive impairment patients after cerebral ischemia and hypoxia, therefore, the recovery of memory and learning ability is always the target of clinical treatment. The experiment uses rats with bilateral common carotid arteries ligated as animal models of cerebral ischemia and anoxia, and adopts water maze experiment to detect the therapeutic effects of faeces Bombycis, human yellow, Oletum Trogopterori, Cassia occidentalis, faeces Vespertilionis, human urine sediment, Cordyceps militaris, Bombyx Batryticatus, periostracum Serpentis, Limonitum, and Pumex on learning and memory injury of rats with cerebral ischemia and anoxia.

(1) Experimental procedure:

the water maze experiment is carried out for 3 times in the experiment, which is respectively before molding, 45 days after molding and 90 days after molding. Each drug is respectively a high dose group (18 g/kg/day), a medium dose group (10 g/kg/day) and a low dose group (4.5 g/kg/day), and a blank control group, a sham operation group and an untreated group are additionally arranged. After adaptive feeding of experimental rats for 3 days, the Morris water maze experiment is formally carried out. The quiet experimental environment was maintained, the temperature of the laboratory was controlled around 24 ℃, a constant humidity of 50 ± 5%, the start and end times of each experiment were consistent, and high consistency of environmental factors was ensured. During the experiment, the color marker on the inner wall of the water pool is kept in a constant position, and any article in a laboratory is not changed. A quiet experimental environment was maintained. The worker does not need to use cosmetics with an irritating taste, such as perfume. The SD rat is placed in the pool at the beginning of the experiment with the SD rat facing the pool with the marker and immediately away from the location where it was standing to avoid the rat erroneously identifying the subject as a marker.

(2) Establishing a rat cerebral ischemia and hypoxia model:

after the water maze experiment before modeling is completed, a rat cerebral ischemia and hypoxia model is manufactured, the rat cerebral ischemia and hypoxia are caused by a permanent bilateral common carotid artery ligation method, and diet is prohibited for 12 hours and drinking water is prohibited for 4 hours before the rat starts modeling. The model is made by injecting 0.4ml/100g of 1% pentoxibital solution into the abdominal cavity for anesthesia, and meanwhile, the rat can breathe autonomously in the whole model making process. The rat is fixed on the experiment table in a supine position, rat hair is cut off from the operation position, the rat hair is carefully cut along the median line of the neck after being sterilized by iodophor, the common carotid arteries on two sides are separated, the two ends of the artery are permanently tied by adopting a No. 0 line operation line, and then the blood vessel is cut off from the middle, so that the arterial blood flow can be fully guaranteed to be blocked. After the postoperative model building is finished, the rat is sent to a cage with a better ventilation environment for continuous feeding.

(4) Injecting a traditional Chinese medicine intestinal flora regulating formula solution into the abdominal cavity:

15g of silkworm excrement, 9g of human middle yellow, 9g of trogopterus dung, 6g of common coffee cherry, 9g of faeces Vespertilionis, 10g of human urine sediment, 3g of cordyceps militaris, 10g of stiff silkworm, 0.6g of snake slough, 15g of limonite and 10g of pumice are respectively decocted to prepare a single-medicine 2.5mg/ml solution, and the single-medicine 2.5mg/ml solution is respectively subjected to intraperitoneal injection on a high-dose group, a medium-dose group and a low-dose group 1-28 days after molding for one time continuously according to the concentrations of 10mg/kg, 5mg/kg and 2.5 mg/kg.

(4) Taking brain tissue

After the water maze experiment is finished, deep anesthesia is carried out on the rat by utilizing a pentobarbital solution, then 0.01MPBS is injected from a heart cavity until liquid flowing out from a right atrium is colorless, then 4% polyfermentate is injected into the rat until the rat body is motionless, the head is cut off, the brain is taken out, frontal lobe and hippocampus area tissues of the brain tissues are collected on an ice box, the frontal lobe and hippocampus area tissues are fixed by 4% polyformic acid, and then the rat is immediately placed in a refrigerator at the temperature of minus 80 ℃ for storage.

The experimental results are as follows:

a rat cerebral ischemia and hypoxia model is caused by a permanent bilateral common carotid artery ligation method, and effective components with strongest protective effects on blood brain barrier resistance and permeability of traditional Chinese medicine components are screened on the basis of widely selecting traditional Chinese medicine intestinal flora regulating medicines according to documents. The traditional Chinese medicine possible regulating medicine for intestinal flora mainly comprises: silkworm excrement, human middle yellow, trogopterus dung, common cassis, faeces of nocturnal emission, human middle white, cordyceps militaris, stiff silkworm, snake slough, limonite, pumice and the like, and screened effective traditional Chinese medicine components comprise the silkworm excrement, the human middle yellow, the trogopterus dung, the common cassis, the human middle white and the cordyceps militaris, and a traditional Chinese medicine intestinal flora regulating formula solution is prepared. The experiment can also show that the higher the concentration of the silkworm excrement, the human middle yellow, the trogopterus dung, the Wanyue sand, the human middle white and the cordyceps militaris drug solution is, the more remarkable the treatment effect on the learning and memory injury of the cerebral ischemia and hypoxia rats is.

The invention further researches the functions of the silkworm excrement, the human Zhonghuang, the trogopterus dung, the Wanyue sand, the human Zhongbai and the cordyceps militaris compound decoction.

15g of silkworm excrement, 9g of human yellow, 9g of trogopterus dung, 6g of cassiabarktree fruit, 10g of human white and 3g of cordyceps militaris are decocted to form a compound, and the high-dose group, the medium-dose group and the low-dose group are subjected to intraperitoneal injection once a day continuously 1-28 days after molding according to the concentration of 10mg/kg, 5mg/kg and 2.5mg/kg respectively. And comparing with a blank control group, a sham operation group and an untreated group. Compared with the method that learning and memory abilities are obtained after the initial water maze is conducted in an untreated group and a sham operation group, the time for searching the platform is obviously shortened after 45 days and 90 days, the rat model group has obvious cognitive dysfunction, and the specific expression is that the duration and the latency period of searching the platform are prolonged and the search distance before the rat enters the platform is obviously increased no matter the rat model is in working memory or positioning cruise. Silkworm excrement, human Zhonghuang, trogopterus dung, expected lunar faeces, human Zhongbai and a cordyceps militaris compound can reduce the duration and latency of a cerebral ischemia and hypoxia rat for searching a platform and obviously shorten the search distance of the model rat before the platform. The experiment can also show that the higher the concentration of the traditional Chinese medicine compound is, the more remarkable the treatment effect on the learning and memory injury of the rats with cerebral ischemia and hypoxia is.

Wsetern blot results: the Caspase-3 content of all cerebral ischemia and anoxia model groups is increased, the LC3B content is reduced, after the cerebral tissue of a rat is subjected to ischemia and anoxia, the excessive autophagy in cells starts an apoptosis process, so that the damage of the cells is aggravated, silkworm excrement, tabasheer, trogopterus dung, faeces bombycis, human urine and cordyceps militaris compound act on the autophagy process of the cells after the model rat is well regulated, and the regulation function is a high dose group, a medium dose group and a low dose group, so that the traditional Chinese medicine intestinal flora regulator has the function of regulating the autophagy process of the cells to relieve the damage of the cerebral tissue subjected to ischemia and anoxia, and the regulation function is more obvious as the dose is higher.

Case section staining observation: silkworm excrement, human yellow, trogopterus dung, expected monthly faeces, human white and medium cordyceps militaris compound high, medium and low dose groups have reduced pathological changes compared with a blank control group.

The brain: the tissue structure of the brain is the same as that of a normal control group, the neuron level of the cortical region is clear, the size is relatively consistent, most cell nuclei are uniformly dyed, the volume of few part of the neuron is slightly reduced, the dyeing of the cell nuclei is deepened, the structure is less clear, slight degeneration is shown, and necrosis does not occur. No edema appeared in the intercellular substance, and no increase of glial cells was observed.

② sea horse: the volume of small centrum cells in the hippocampus 1 is reduced, the cell nucleus is solidified and shrunk, the structure is less clear, the shape is irregular, and the arrangement of local areas is disordered. Other regions hippocampal tissue structures were essentially normal, and no significant degeneration or necrosis of the vertebral cells occurred.

Example 2 screening of effective drugs of the traditional Chinese medicine intestinal flora regulating formula and observation of curative effects of the A beta 25-35 on mouse senile dementia models.

According to the theory of traditional Chinese medicine, Abeta has the characteristics of corrupting body and damaging brain marrow, and belongs to the category of endogenous turbid toxin generated by human body. The accumulation of A beta in brain caused by peripheral clearing dysfunction of A beta liver is consistent with the pathogenesis of 'toxicity damage to brain collaterals' caused by 'smart spleen disorder' of 'failure syndrome' in traditional Chinese medicine. We speculate that traditional Chinese medicine intestinal flora regulating drugs can improve cognitive functions and pathology of APP/PS1 mice and reduce A beta levels in brain.

(1) The experimental method comprises the following steps:

experimental grouping and dosing: 15g of silkworm excrement, 9g of human yellow, 9g of trogopterus dung, 6g of cassiae toralis, 9g of faeces Vespertilionis, 10g of human Chinese cabbage, 3g of cordyceps militaris, 10g of stiff silkworm, 0.6g of snake slough, 15g of limonite and 10g of pumice are respectively prepared into a low dose and a high dose, the low dose is 2mg/kg per day, the high dose is 10mg/kg per day, the treatment period is four weeks, and water maze detection is carried out every week.

(2) Detection indexes and detection methods:

model a β 25-35: mice were anesthetized by intraperitoneal injection of 3% sodium pentobarbital (40mg/kg), fixed in a brain stereotaxic apparatus, and pre-halogen was exposed, and 3 μ L A β 25-35 was injected into the right ventricle of each mouse, referring to the stereotaxic map of the mouse brain. The animals are raised in cages after being conscious.

Morris water maze

A water pool: a circular shape with a diameter of about 120cm and an overall height of 60 cm; a platform with the diameter of 10 cm is arranged in the device, the height of the platform is slightly lower than the height of the pool (by 8-10 cm), the position of the platform is slightly lower than the water level in the pool (by 1 cm), meanwhile, the water humidity in the pool is processed at a constant temperature, and is controlled at 22 ℃, and the error range is +/-1 ℃.

HE staining

Firstly, paraffin sections are prepared, then the prepared sections of the mouse brain are stained and compared, and two staining agents are respectively hematoxylin staining solution and eosin staining solution.

(3) The experimental results are as follows:

the senile dementia model mouse has certain improvement after administration of human yellow, human white, trogopterus dung, silkworm excrement, Wanyue sand, Cordyceps militaris and bat dung, wherein the improvement of a high-dose group is obvious, the bat dung group has occasional anaphylactic reaction, and the stiff silkworm, the snake slough, the limonite and the pumice have no obvious cognitive improvement effect. The distance from each group of mice to the designated platform is shortened along with the increase of the learning time of the mice, the senile dementia model mice are improved to a certain extent after being administrated with the traditional Chinese medicine of human Zhonghuang, the traditional Chinese medicine of human Zhongbai, the trogopterus dung, the silkworm excrement, the expected month sand, the cordyceps militaris and the faeces bombycis, and the improvement of a high-dose group is obvious. The effective components of the composition are yellow in human body, white in human body, Oletum Trogopterori, faeces Bombycis, faeces Vespertilionis, faeces Bombycis, and Cordyceps. HE results show that normal mice hippocampal centrum nucleus is round, clear in outline, regular in arrangement and uniform in coloring. The hippocampal pyramidal cells of the mice in the model group are arranged more loosely and disorderly, the number of cell layers is reduced, and the arrangement is irregular. The low-dose group and the high-dose group are respectively given to the human Zhonghuang, the human Zhongbai, the trogopterus dung, the silkworm excrement, the hope moon sand, the cordyceps militaris, the bat dung, the stiff silkworm, the snake slough, the limonite and the pumice decoction, and the observation shows that the human Zhonghuang, the human Zhongbai, the trogopterus dung, the silkworm excrement, the hope moon sand and the cordyceps militaris decoction can obviously improve the arrangement and the number of the hippocampal pyramidal cells, and prompts that the hippocampal neuronal injured cells have a certain improvement effect. The night soil decoction has the effect of improving at a high dosage, does not improve at a low dosage, and does not improve the muscardine silkworm, the snake slough, the limonite and the pumice obviously.

Example 3 screening of effective components of a possible modulating drug of the intestinal flora of traditional Chinese medicine.

Selecting an AP/PS-1 double-transgenic mouse, converting the dosage into oral administration dosage of effective traditional Chinese medicine components according to the common dosage of traditional Chinese medicines for clinical application, randomly dividing the double-transgenic mouse into a high-dosage group, a medium-dosage group, a low-dosage group and a control group, and carrying out oral administration for 3 months. After the administration is finished, the Morris water maze test method is adopted to carry out positioning cruise training on the mouse, the training is carried out for 4 times every day, the interval is 1h every time, the training lasts for 4 days, then the positioning navigation test and the space exploration test are carried out, and the acquisition capability of the mouse on learning and memory and the memory retention capability of the mouse on the space position of the platform are detected. Studies have shown that drugs effective in reducing the distance time a mouse reaches the platform include: yellow human middle, white human middle, trogopterus dung, silkworm excrement, faeces Bodinieri, faeces Vespertilionis and Cordyceps militaris.

Example 4 the curative effect of silkworm excrement, human yellow, trogopterus dung, Wanyue sand, human white and Cordyceps militaris compound is compared with that of the existing traditional Chinese medicine intestinal flora regulator.

20 +/-2 g of Kunming female mice are selected and randomly divided into a normal group and a high-fat group, drinking water is normally taken, after adaptive culture is carried out for 1 week, the normal group is fed with a normal diet, and 45% kcal of high-fat mouse food is used in the high-fat group. Body weights were measured once a week, and after 4 weeks mice were sacrificed at random per group and dissected under sterile conditions. Taking colon, longitudinally cutting open with scissors, lightly scraping with slide to obtain intestinal mucosa tissue, collecting in EP-free tube, and storing in-80 deg.C refrigerator for subsequent experiment. The colon is taken under sterile environment, and the intestinal mucosa tissue is taken out by lightly scraping the slide.

The high fat group was randomly divided into: the existing intestinal flora regulating traditional Chinese medicine (folium sennae group), normal saline group, faeces bombycis, human middle yellow, Oletum Trogopterori, faeces bombycis, human middle white, and Cordyceps militaris compound group, and folium sennae group is administered with 0.2ml folium sennae water decoction for intragastric administration for 1 time; the normal saline group is administrated with 0.2ml of normal saline for intragastric administration for 1 time; the compound group converts the clinical dosage of human into the experimental dosage of the group of mice according to the weight of the group of mice, and the mice are subjected to intragastric administration once. Mice were observed for defecation at the next 12h and recorded. After 12h, the high-fat mouse grains of three groups of mice are replaced by common mouse grains for continuous feeding, meanwhile, the mice of each group are sacrificed randomly, colon tissues with the size of 3x3mm are fixed in 4 percent paraformaldehyde solution, and surrounding colon mucosa tissues are collected and placed in a sterile EP tube for preservation at the temperature of minus 80 ℃.

The results show that the mice in the high-fat group gradually change, the hair color is greasy, the mice are in locks, compared with the mice in the normal group, the mental state is inactive, the resistance is weak, the color of the excrement is light, the excrement is placed in water, the visible water surface floats with oil flowers, compared with the normal control group, the high-fat group has obvious partial flora deletion, after the traditional Chinese medicine is adjusted by using the flora, the normal saline group is recovered slowly, the senna leaf group is recovered quickly, and the compound group of silkworm excrement, human chinese rhubarb, trogopterus dung, expected monthly sand, human Chinese white and cordyceps militaris is recovered fastest and is superior to the senna leaf group and the normal saline group.

Example 5 effective formulation rules for subacute aging rat models were selected from human chinese rhubarb, human chinese cabbage, trogopterus dung, silkworm excrement, faeces of hopeful moon, cordyceps militaris, faeces of nocturnal emission, stiff silkworm, snake slough, limonite and pumice.

Selecting 60 SPF grade healthy adult female Wister rats with the weight of 200 +/-20 g, and performing subcutaneous injection of D-galactose for 1 time every day for 30 consecutive days to prepare a subacute aging rat model. The cognitive level of the model rats is evaluated by the Morris water maze, and the rats are determined to be in a cognitive disorder state. The Chinese rhubarb, the Chinese cabbage, the trogopterus dung, the silkworm excrement, the Wanyue sand, the cordyceps militaris, the bat dung, the stiff silkworm, the snake slough, the limonite and the pumice are respectively administrated to the stomach, and the high dose and the low dose can shorten the escape latency of the aged rats as shown in the Morris water maze experiment: the traditional Chinese medicine composition comprises human middle yellow, human middle white, trogopterus dung, silkworm excrement, Wanyue sand and cordyceps militaris, wherein the high dose can be shortened, the low dose has no effect of nocturnal emission, and the high dose and the low dose have no obvious cognitive improvement effect of stiff silkworm, snake slough, limonite and pumice.

Example 6 improved compound composition for vascular cognitive disorders was determined.

ApoE-/-mice and C57BL/6J mice with the same genetic background, 8 weeks old, body mass 20 + -2 g, C57BL/6J mice were selected to be fed with the normal mouse feed as a blank control group. ApoE-/-mice were selected to be fed with high fat diet as a model control group. The traditional Chinese medicine comprises a compound group of Erchen decoction and Taohong Siwu decoction, a compound group of human middle yellow, human middle white, faeces Trogopterori, faeces bombycis and Cordyceps militaris, and a compound group of human middle yellow, human middle white, faeces Trogopterori, faeces bombycis, Cordyceps militaris and faeces Vespertilionis.

After-treatment Morris water maze detection results show that: in a positioning navigation experiment, compared with a model group, the escape latency and the swimming distance of the traditional Chinese medicine are obviously reduced, and the traditional Chinese medicine is most obviously prepared from human yellow, human white, trogopterus dung, silkworm excrement, plenilune sand and cordyceps militaris; yellow human middle, white human middle, trogopterus dung, silkworm excrement, faeces Bodinieri, Cordyceps militaris, faeces Vespertilionis; the Erchen decoction and Tao hong Siwu decoction are inferior to the first two groups.

Compared with the model group, the traditional Chinese medicine group has obviously increased times of crossing the platform, and the times of crossing the yellow in the middle of human body, the white in the middle of human body, the trogopterus dung, the silkworm excrement, the cassiae strelittoralis and the cordyceps militaris group are more obviously increased.

Example 7 formulation components for vascular cognitive disorders validation.

Selecting a male ICR mouse with the weight of 30-35 g, and utilizing a persistent bilateral common carotid artery ligation method to cause a cognitive disorder model caused by rat whole brain hypoperfusion: each mouse was marked and weighed and then the abdominal cavity was injected with chloral hydrate and surgery was performed after the mouse entered the state of anesthesia. The method comprises the steps of fixing a mouse in a supine position, disinfecting the neck by a cotton ball soaked in iodine, cutting a small opening of 3-5 cm along the central line of the neck, slightly separating mucous membranes connecting two submaxillary glands by using forceps, then cutting an arterial sheath of a common carotid artery on one side to expose the common carotid artery, separating the common carotid artery from a vagus nerve, placing a suture line at the far end below the common carotid artery, slightly lifting the common carotid artery by using hemostatic forceps to clamp the suture line, then placing a spring ring soaked in 75% ethanol below the common carotid artery by using the forceps, and slightly shifting the common carotid artery to wind the artery into the spring ring. The suture was gently removed and the common carotid artery was placed back in place. And performing the operation on the other side. The neck opening of the mouse is sutured well by a suture after the operation on the other side is finished.

The Morris water maze test was performed on mice 1 month after surgery, and the first part was a platform visualization experiment, i.e. adaptive training of the mice. The second part is a spatial memory acquisition experiment (also called positioning cruise experiment) in which the platform is hidden in water. The third part is the space exploration experiment, in which the platform is removed. The diameter of the mouse pool is 120cm, the height of the mouse pool is 40cm, the temperature in the mouse pool is kept at 25 +/-1 ℃, and water can circulate to balance the water temperature. Preparing a traditional Chinese medicine intestinal flora regulating formula from 8g of human Zhonghuang, 9g of human Zhongbai, 9g of trogopterus dung, 12g of silkworm excrement, 5g of Wanyue sand and 3g of cordyceps militaris according to the weight of a mouse, and observing that the escape latency of the traditional Chinese medicine mouse is obviously shorter than that of a control group.

Through the experiment, the formula of the medicinal composition is determined as follows: silkworm excrement, human yellow, trogopterus dung, expected monthly faeces, human white and cordyceps militaris.

Example 8 use in non-dementia type vascular cognitive disorders.

32 patients with eligible non-dementia type vascular cognitive impairment are screened by Montreal cognitive assessment scale, Hanchinski ischemia scoring scale and clinical dementia assessment scale. Inclusion criteria met the cognitive criteria for vascular cognitive impairment as established by the neurological division of the chinese medical society in 2011.

The traditional Chinese medicine intestinal flora regulating formula (7 g of human Zhonghuang, 6g of human Zhongbai, 6g of trogopterus dung, 12g of silkworm excrement, 5g of expected monthly sand and 3g of cordyceps militaris) is used by patients in the group for decoction for half an hour for treatment, 1 course of treatment is performed in 3 months, repeated diagnosis is performed for 1 time in 3 months, and a daily life capacity scale, a behavior scale, an senile dementia scale, a vascular dementia dialectical scale and a blood homocysteine value are evaluated.

After 3 months of treatment, 17 cases are effective, 15 cases are ineffective, 0 case is worsened, and the total effective rate is 53.13%; after 6 months of treatment, 28 cases are effective, 3 cases are ineffective, 1 case is worsened, and the total effective rate is 87.5%.

Example 9 Effect on serum A.beta.1-42 and NPY concentration in patients with vascular cognitive impairment.

140 patients met the vascular cognitive disorder diagnosis criteria (Rockwood K et al criteria). Performing neuropsychological evaluation on the patient by applying a neuropsychological scale, extracting 5ml of fasting venous blood in the early morning, performing heparin anticoagulation, centrifuging at 2000r/min for 15min, completely separating blood plasma, and storing in a refrigerator at the temperature of-80 ℃. The Elisa method measures serum A beta 1-42 and NPY concentration.

Referring to the Chinese guidelines for cerebrovascular disease prevention and treatment, basic western medicine treatment is applied to cerebrovascular risk factors, including blood pressure control, blood sugar control, platelet aggregation resistance, plaque stabilization and the like. Adding Chinese medicinal intestinal flora regulating formulation (9 g of human yellow, 5g of human white, 5g of trogopterus dung, 15g of silkworm excrement, 6g of faeces Bombycis and 3g of Cordyceps militaris), decocting with water for half an hour, 1 dose per day, and taking twice in the morning and evening, with 24 weeks as a treatment course.

69 cases of obvious effect after 24 weeks of treatment, 52 cases of effect, 17 cases of no effect, 2 cases of deterioration and 86.43% of total effective rate, 5mL of fasting venous blood is extracted before and after treatment in the morning, and the concentration of serum Abeta 1-42 and NPY is measured, so that the concentration of serum Abeta 1-42 is obviously increased, and the level of NPY is obviously reduced.

Example 10 effects on neuropsychological scale and blood homocysteine levels in patients with vascular cognitive impairment.

The existing cerebrovascular disease history is evaluated by a Montreal cognition evaluation scale, a clinical dementia evaluation scale and a Hanchinski ischemia scale, patients meeting the inclusion standard are screened out, then a daily life capacity scale, a Blessed behavior scale, an senile dementia evaluation scale and a traditional Chinese medicine syndrome symptom scoring scale are carried out one by one, fasting serum is collected, and the blood homocysteine level is measured.

The preparation is taken by 64 patients with vascular cognitive disorder, and is prepared by adding traditional Chinese medicine intestinal flora regulating prescription (6 g of human Zhonghuang, 6g of human Zhongbai, 6g of trogopterus dung, 10g of silkworm excrement, 5g of Wang month excrement and 3g of cordyceps militaris) on the basis of individually taking antihypertensive and hypoglycemic drugs, statins, antiplatelet aggregation drugs and drugs for improving myocardial ischemia, decocting with water for half an hour, 1 dose per day, and taking the decoction warm in the morning and evening. 4 weeks are used as 1 treatment course, and 3 treatment courses are observed.

The cognitive component table scores of the daily living capacity scale, the Blessed behavior scale and the senile dementia rating scale of the patient are effectively improved, the blood homocysteine level is obviously reduced, 28 cases are obviously effective, 24 cases are effective, 11 cases are ineffective, 1 case is deteriorated, and the total effective rate is 81.25%.

Example 11 clinical efficacy on patients with vascular cognitive impairment in combination with sleep disorders.

Screening patients by adopting general survey, including 240 patients meeting the condition vascular cognitive disorder, and screening 94 patients meeting the condition sleep disorder from the 240 patients, wherein the diagnosis standard adopts the vascular cognitive disorder diagnosis standard provided by Rockwood and the like, and the sleep disorder lasts for more than 1 month.

The traditional Chinese medicine intestinal flora regulating formula (8 g of human Zhonghuang, 8g of human Zhongbai, 6g of trogopterus dung, 12g of silkworm excrement, 5g of Wanyue sand and 3g of cordyceps militaris) is given, the traditional Chinese medicine intestinal flora regulating formula is decocted with water for 3 times per day, the treatment course is 12 weeks, and the scale detection is completed within 1 week before the treatment and 1 week after the treatment is finished respectively.

94 patients with vascular cognitive disorder and sleep disorder are treated by using the traditional Chinese medicine intestinal flora regulating formula, 32 patients with vascular cognitive disorder and sleep disorder have obvious effect, 41 patients with effective effect and 21 patients with ineffective effect, and the total effective rate is 77.66%.

Example 12 intervention in mild to moderate vascular cognitive disorders in combination with cognitive training.

The traditional Chinese medicine intestinal flora regulating formula (6 g of human Zhonghuang, 6g of human Zhongbai, 6g of trogopterus dung, 10g of silkworm excrement, 5g of Wanyue sand and 3g of cordyceps militaris) is given, 1 dose is taken in the morning and evening. Combination training interventions, including home training interventions 1 time per day, with medical personnel training interventions 1 time per week.

The senile dementia rating scale, the blisssed behavior scale and the daily life capacity scale were observed. The main therapeutic index is recorded 1 time by each observation on the last 1 day of the 3 rd and 6 th months. The safety observation indexes include blood and urine routine, stool routine, electrocardiogram, liver and kidney function, etc. The home training intervention comprises: attention training; secondly, memory training; training directional force; fourthly, drawing the clock for training. The medical personnel training intervention comprises: firstly, spatial perception training; secondly, exercise training; and thirdly, thinking operation training.

The traditional Chinese medicine intestinal flora regulator and cognitive training technology are adopted to intervene in 360 patients with mild and moderate vascular cognitive impairment, cognitive training comprises family training intervention 1 time every day, medical personnel training intervention 1 time every week, 162 cases with obvious effect, 137 cases with effect, 49 cases with no effect, 12 cases with deterioration and 83.06% of total effective rate.

Example 13 early intervention hypertension treatment improves cognitive impairment.

And observing the curative effect of the traditional Chinese medicine intestinal flora regulating formula on improving cognitive impairment by early intervention of hypertension treatment, wherein the curative effect indexes comprise a simple mental state examination scale, a vascular dementia cognitive assessment scale, a daily life capacity scale and a clinical dementia grading scale.

Patients with hypertension who meet the diagnostic criteria of 2016 (Canadian guidelines for hypertension control) edition were included, and the cognitive impairment criteria met the clinical guidelines for mild cognitive impairment in 2008 (draft). The blood pressure control scheme adopts calcium ion channel blocker, angiotensin converting enzyme inhibitor and other hypotensor which can control blood pressure and has no damage to brain cognitive function, and the traditional Chinese medicine intestinal flora regulating prescription (10g silkworm excrement, 9g human middle yellow, 6g trogopterus dung, 6g pleiomorpha, 5g human middle white and 3 parts cordyceps militaris) is given, 1 dose is taken every day in three times, 6 months are taken as 1 treatment course, and 1 treatment course is continuously treated.

The traditional Chinese medicine intestinal flora regulating formula is used for early intervention hypertension treatment to improve cognitive impairment, and is included in 64 patients with mild cognitive impairment of hypertension, wherein 28 patients have obvious effect, 22 patients have effect, 14 patients have no effect, and the total effective rate is 78.13%.

Example 14A Chinese medicinal gut flora modulating formula for the treatment of adrenoleukodystrophy.

The patient was male, 40 years old, and was diagnosed for the first time in 2018, 6 months and 21 days. Patients consciously feel hypodynamia of the two lower limbs in 2015, walk unstably, then symptoms gradually aggravate with lack of speech, visit Shanghai Huashan Hospital twice from 11 months to 3 months in 2018 in 2017, and seek MRI enhancement + DWI show that: the bilateral cerebellum and bridge arm, bilateral lateral ventriculo-ventricular and basal ganglia region patch-shaped abnormal signal shadow are considered, and active white matter degeneration diseases are considered; the cerebellum is slightly atrophied. Electromyography: upper and lower limb somatosensory conduction pathway impairment considerations. The adrenal leukodegeneration related gene is positive. First diagnosis: hypomnesis, choking cough due to drinking water, weakness of both lower limbs, dysuria, fecal incontinence, pale tongue with thin and yellow coating, and weak pulse. The professor in the Chaxinzhen gives treatment of invigorating qi, promoting blood circulation and nourishing blood on the basis of nourishing nerves and improving circulation, and the following medicines are used: 20g of astragalus, 10g of cassia twig, 15g of fried white peony root, 10g of medicinal cyathula root, 10g of radix achyranthis bidentatae, 10g of eucommia bark, 60 doses of the medicinal preparation, 1 dose a day and 2 times in the morning and at night. And B, diagnosis: in 2018, 10 and 12 months, the patient has weak lower limbs, walking instability is not obviously improved, the symptoms of slurred speech and choking cough are slightly improved compared with the former symptoms, the symptoms such as fecal incontinence and dysuria are still remained, and the traditional Chinese medicine intestinal flora regulating formula is given to the patient with the consideration of the white matter degeneration of the patient, the treatment of regulating the brain-intestinal axis is carried out, the cognition is improved, and the formula is adjusted and added: 10g of silkworm excrement, 6g of tabasheer, 9g of trogopterus dung, 6g of cassiabarktree fruit, 90 doses of faeces bombycis, 1 dose a day, and taken separately in the morning and evening for 2 times. Three diagnoses: 26 months in 2019, the symptoms of the patient are obviously improved compared with the prior symptoms, the excrement is still adjusted, the urine is still clear, the patient is ordered to continue the treatment of the traditional Chinese medicine and intestinal flora adjusting formula, 30 doses are taken every other day for 1 dose, the administration is carried out 2 times in the morning and at night, the exercise is appropriately ordered, and the psychosomatic rehabilitation is strengthened.

Example 15A Chinese medicinal intestinal flora regulating formulation for treating acute ischemic-hypoxic encephalopathy.

The patient was male, 81 years old, and the initial diagnosis was 12 months and 21 days in 2018. After meals in 12 and 16 days, patients have dizziness, nausea and vomiting, have the history of hypertension and are treated in the department of China. Skull-finding CT: 1. cerebral infarction of left cerebellar hemisphere may occur; multiple old coelomic peduncles in the brain; 2. senile brain changes; bilateral paraventricular ischemic changes; the intracranial segment of the bilateral internal carotid artery is provided with multiple calcifications, and anticoagulation, platelet aggregation resistance and the like are given. In 12 months and 26 days, the glutinous rice balls are sucked into the air passages by mistake due to eating of the glutinous rice balls, sudden heartbeat and sudden respiratory arrest, the lips are purple, the massive objects are sucked out through phlegm suction and cardio-pulmonary resuscitation, and vital signs are stable after the qi-moving tubes are inserted into the tubes. First diagnosis: clear mind, lassitude, dark tongue with yellow and thick coating, and weak pulse. On the basis of resisting infection, reducing phlegm and supporting nutrition, the professor in the stannum town gives the treatments of promoting blood circulation by removing blood stasis and inducing resuscitation and restoring consciousness, and the following medicines are used: 10g of buffalo horn, 30g of red paeony root, 30g of salvia miltiorrhiza, 10g of cortex moutan, 10g of dried rehmannia root, 10g of prepared rhubarb, 6g of irkutsk anemone rhizome, 10g of radix curcumae, 14 doses, 1 dose a day and 2 times in the morning and at night. And B, diagnosis: in 2019, day 1 and 21, sputum of a patient is reduced compared with the former sputum, the calculation power, the comprehension and the memory are not improved obviously, cough still exists, the color of urine is dark, a traditional Chinese medicine intestinal flora regulating formula is considered to be added, brain-intestinal axis treatment is regulated, 15g of silkworm excrement, 9g of human yellow, 9g of trogopterus dung, 6g of common coffee cherry, 30 doses of silkworm excrement and 1 dose of urine are added in an original formula, and the medicine is taken 2 times in the morning and at night. Three diagnoses: in 2019, 16 months 2, the symptoms of the patient are obviously improved, the excrement is still adjusted, the urine is still clear, the patient is ordered to continue the treatment of the traditional Chinese medicine and intestinal flora adjusting formula, and the formula medicines are as follows: 10g of silkworm excrement, 6g of human yellow, 6g of trogopterus dung, 3g of Wanyueshao, 30 doses of Wanyueshao, 1 dose every other day, and taken separately in the morning and evening for 2 times, with the order of moderate exercise, and the strengthening of psychosomatic rehabilitation.

Example 16A traditional Chinese medicine gut flora regulating formulation for the treatment of gut flora shifting encephalopathy.

The patient was male, 80 years old, with an initial diagnosis of 2016, 1, 7 days. The patient has slow reaction in 6 days after 12 months, and is choked to drink water, and the left lower limb is painful. The body was examined to see that the left lower limb was scattered on herpes. Checking 1.5T craniocerebral magnetic resonance flat scan + DWI + FLAIR: 1. old cavity peduncles in bilateral basal ganglia regions; multiple ischemic changes in bilateral cerebral hemispheres; brain atrophy; 2. there was little inflammation of the right mastoid. Electroencephalogram examination: a wide range of severe abnormalities electroencephalograms. Pathogenic microorganism gene: leuconostoc citreum. First diagnosis: slow reaction, dry stool, urinary incontinence, dark tongue with white coating, and wiry and slippery pulse. On the basis of resisting infection and restoring consciousness and inducing resuscitation, the professor in the stannum town is added with the prescription for clearing lung and eliminating phlegm and regulating intestinal flora of traditional Chinese medicines, and the prescription is as follows: 10g of cortex mori radicis, 3g of platycodon grandiflorum, 10g of common coltsfoot flower, 30g of gypsum, 6g of baical skullcap root, 10g of prepared rhubarb, 10g of dried rehmannia root, 15g of silkworm excrement, 9g of rhinestone, 9g of trogopterus dung, 6g of coffee senna, 14 doses, 1 dose in the day and 2 times in the morning and evening. And B, diagnosis: in 2016, 1 month and 21 days, the comprehension, the calculation and the memory of a patient are obviously improved compared with the prior art, the patient still has fever, the patient is bitter in the Chinese medicament, and the single Chinese medicament intestinal flora regulating formula is used for treating the fever, and the formula comprises the following medicaments: silkworm excrement 15g, chinese rhubarb 9g, trogopterus dung 9g, coffee senna 6g, 14 doses, 1 dose a day, morning and evening for 2 times. Three diagnoses: in 2016, 2, 4 days, the symptoms of the patient are obviously improved, and the patient is ordered to continue the treatment of the traditional Chinese medicine intestinal flora regulating formula, wherein the formula comprises the following medicines: 10g of silkworm excrement, 6g of Renzhonghuang, 6g of trogopterus dung, 4g of Cassia occidentalis, 30 doses of faeces Bombycis, 1 dose every other day, and taken separately in the morning and evening for 2 times, with the advice of moderate exercise, and the enhancement of psychosomatic rehabilitation.

Example 17 intravenous thrombolysis in combination with a traditional Chinese medicine intestinal flora modulating formulation treats acute ischemic stroke.

The patient female, 94 years old, was first diagnosed in 2017, 9 months and 13 days. The patient suffered from fatigue in the morning of 9 and 13 months, and had left limb weakness, facial distortion and left side hemianopsia of the right eye. Skull-finding CT: lacunar infarct foci. It is used for treating venous thrombolysis in emergency treatment. Skull-finding MRI: 1. acute infarction of the right temporal occipital lobe, the basal ganglia. 2. Ischemic changes in the white matter of the brain, mild brain atrophy. First diagnosis: impotence, dizziness, headache, slow reaction, choking cough due to drinking water, red tongue with yellow and greasy coating and slippery pulse. On the basis of protecting brain and removing free radicals, the professor in the Chaxinzhen is added with the prescription for promoting blood circulation, removing blood stasis and dredging collaterals and the traditional Chinese medicine intestinal flora regulation, and the prescription and the medicine are as follows: 30g of red peony root, 10g of cortex moutan, 30g of salvia miltiorrhiza, 3g of leech, 10g of earthworm, 15g of silkworm excrement, 9g of human yellow, 9g of trogopterus dung, 6g of cassiae streptalis, 14 doses of faeces bombycis, 1 dose a day and 2 times in the morning and at night. And B, diagnosis: in 2017, in 27 months 9, the left limbs of the patient improve the movement, the drinking water is not choked, the speech is clear, the memory is slightly declined, and the prescription for opening orifices of tongue and regulating intestinal flora is given, which specifically comprises the following steps: 6g of irkutsk anemone rhizome, 10g of radix curcumae, 6g of polygala root, 10g of rhizoma gastrodiae, 10g of mulberry twig, 10g of silkworm excrement, 6g of rhizia chinensis, 6g of trogopterus dung, 3g of cassiabarktree fruit, 14 doses, 1 dose a day and 2 times in the morning and evening. Three diagnoses: in 2017, 10 and 11 months, the symptoms of the patient are obviously improved compared with the symptoms of the patient before, the excrement is well regulated, the urine is well regulated, and the patient is ordered to take a single traditional Chinese medicine intestinal flora regulating formula for treatment, wherein the formula comprises the following medicines: 10g of silkworm excrement, 6g of Renzhonghuang, 5g of trogopterus dung, 3g of Cassia occidentalis, 30 doses of faeces Bombycis, 1 dose every other day, and taken separately in the morning and evening for 2 times, with the advice of moderate exercise, and the enhancement of psychosomatic rehabilitation.

Example 18 mechanical artery embolectomy in combination with traditional Chinese medicine intestinal flora modulation to treat acute ischemic stroke.

The patient was male, 60 years old, with a first visit at 11/2/2018. The patients have tongue stiffness, speech difficulty and right limb movement difficulty in 11-month and 2-morning hours, the skull-checking CT + CTA + CTP is used for flatly scanning mixed plaques at the aortic arch and the head-arm trunk starting part, and the lumens are slightly narrow; the left internal carotid artery neck segment, rock segment and rupture hole segment are blocked, and further inspection by DSA is recommended; mixed plaque at the left common carotid artery bifurcation, and mild stenosis of the lumen; left middle cerebral artery M2-4 segment occlusion; the thyroid nodules on the right side, please combine with ultrasound examination; 3. head CT perfusion display: left frontotemporal parietal occipital lobe was less perfused than contralateral (consistent with pre-infarction stage II-2 CT perfusion performance), suggesting MRI for further examination. 4. A right side radiation crown area softening stove; bilateral maxillary sinusitis. The treatment of acute intracranial artery interventional embolectomy, postoperative administration for resisting platelet aggregation, eliminating free radicals, protecting brain and the like. First diagnosis: impotence, loss of speech, dark red tongue with thin and white coating and wiry and slippery pulse. On the basis of promoting blood circulation to remove blood stasis, removing phlegm and dredging collaterals by professor in the Chaxinzhen, a traditional Chinese medicine intestinal flora regulating formula is added for treatment, and the formula and the medicine are as follows: 6g of arisaema cum bile, 10g of pinellia ternate, 10g of gastrodia elata, 15g of uncaria, 30g of lapis chlorite-schist, 3g of leech, 10g of earthworm, 30g of suberect spatholobus stem, 10g of silkworm excrement, 9g of rhixanthus chinensis, 9g of trogopterus dung, 6g of coffee senna, 14 doses, 1 dose in the morning and evening, and is taken separately for 2 times in the morning and at night. And B, diagnosis: in 2018, in 16 days 11 and 16, the grip strength of the right hand of the patient is slightly reduced, the speech is slightly improved compared with the speech, and the qi tonifying and lifting and the traditional Chinese medicine intestinal flora regulating formula are continuously used for treating, wherein the formula comprises the following medicines: 20g of astragalus membranaceus, 30g of raw bighead atractylodes rhizome, 6g of radix bupleuri, 6g of rhizoma cimicifugae, 10g of ramulus mori, 10g of silkworm excrement, 9g of rhizoma rheum officinale, 9g of trogopterus dung, 6g of faeces cassiae, 14 doses of faeces bombycis, 1 dose a day, and taken 2 times in the morning and evening. Three diagnoses: in 2018, in 11 and 30 months, the symptoms of the patient are obviously improved, and the patient is ordered to continue the treatment of the traditional Chinese medicine intestinal flora regulation formula, wherein the formula comprises the following medicines: 10g of silkworm excrement, 6g of Renzhonghuang, 5g of trogopterus dung, 3g of Cassia occidentalis, 30 doses of faeces Bombycis, 1 dose every other day, and taken separately in the morning and evening for 2 times, with the advice of moderate exercise, and the enhancement of psychosomatic rehabilitation.

Example 19 anticoagulation in combination with traditional Chinese medicine intestinal flora modulating formulations for the treatment of acute central retinal artery occlusion.

The patient was male, 70 years old, with an initial visit of 2016, 12 months and 13 days. The patient suffered from headache, nausea, right eye sudden blindness, and head-finding CT (computed tomography) in the morning of 12 months and 6 days: 1. ventriculo-ventricles on both sides and luminal peduncles and ischemic foci on both sides of basal ganglia, 2. senile brain changes. Head and neck CTA + craniocerebral CTP: aortic arch and branch mixed plaque thereof, left subclavian artery initial part lumen mild stenosis; mixed plaques at the middle section of the left common carotid artery and the branches of the two common carotid arteries, and the lumen is slightly narrow; mixed plaque at the starting part of the right internal carotid artery and moderate stenosis of the lumen; calcified plaque in bilateral internal carotid artery and vertebral artery intracranial segment, no obvious stenosis in lumen; suspicious nodules behind the left internal carotid artery traffic segment consider possibility of bed-process hyperosteogeny, and the small aneurysm is eliminated by DSA (digital radiography) examination if necessary; no obvious abnormality is found in the skull CT perfusion; collapse of the right frontal bone with subcutaneous nodules, please combine clinically; two lungs were multinodular and a chest CT examination was suggested. The phenotype of the APOE-E2/E4-APOE3 genotype is E3 type, the risk of senile dementia is higher than that of APOE-E3/E3+ E2 type, and lower than that of E4 type, so that the method has no obvious tendency to the occurrence of cardiovascular and cerebrovascular diseases and has little selection relation to lipid-lowering medicaments. Anticoagulant and platelet aggregation resisting treatment. First diagnosis: sudden blindness of the right eye, headache, nausea, vomiting, pale tongue with white and greasy coating, and slippery pulse. On the basis of promoting blood circulation to remove blood stasis, warming yang to dispel cold, eliminating phlegm and inducing resuscitation, the professor in the town of stannum is added with a traditional Chinese medicine intestinal flora regulating formula for treatment, and the formula and the medicine are as follows: 3g of ephedra, 10g of deerhorn glue, 10g of medlar, 3g of leech, 10g of earthworm, 10g of safflower, 10g of silkworm excrement, 9g of human yellow, 9g of trogopterus dung, 6g of cassiae torae, 14 doses, 1 dose a day and 2 times in the morning and at night. And B, diagnosis: in 2016, 12 months and 27 days, the patient has a visual index of 15cm for the right eye, and has a feeling of numbness at the top of the head, and the traditional Chinese medicine intestinal flora regulating formula is continuously used for treatment, wherein the formula comprises the following medicines: 10g of medlar, 10g of raspberry, 6g of schisandra chinensis, 30g of salvia miltiorrhiza, 30g of rhodiola rosea, 15g of silkworm excrement, 9g of rhizine, 9g of trogopterus dung, 6g of cassiae streptacantharis, 30 doses, 1 dose a day and 2 times in the morning and at night. Three diagnoses: in 2016, 1 month and 28 days, the symptoms of the patient are obviously improved, the stool is still adjusted, the urine is still clear, and the patient is ordered to continue the treatment of the traditional Chinese medicine intestinal flora adjusting formula, wherein the formula comprises the following medicines: 10g of silkworm excrement, 6g of human yellow, 6g of trogopterus dung, 3g of Wanyueshao, 30 doses of Wanyueshao, 1 dose every other day, and taken separately in the morning and evening for 2 times, with the order of moderate exercise, and the strengthening of psychosomatic rehabilitation.

In conclusion, the traditional Chinese medicine composition disclosed by the invention is simple in formula and wide in source, can effectively improve vascular cognitive dysfunction, and has a certain intervention effect on various accompanying risk factors such as hypertension, hypercholesterolemia, diabetes, metabolic syndrome, atherosclerosis and the like.

Claims

1. The traditional Chinese medicine composition for regulating intestinal flora is characterized by comprising the following components in parts by weight: 10-15 parts of silkworm excrement, 6-9 parts of tabasheer, 4.5-9 parts of trogopterus dung and 3-6 parts of cassiae toralis.

2. The traditional Chinese medicine composition according to claim 1, wherein the pharmaceutical composition comprises the following components in parts by weight: 10-15 parts of silkworm excrement, 6-9 parts of rhus chinensis, 4.5-9 parts of trogopterus dung, 3-6 parts of cassiae streptacanthae, 5-10 parts of human urine and 3 parts of cordyceps militaris.

3. Use of a Chinese medicinal composition according to claim 1 or 2 in the manufacture of a medicament for ameliorating a condition associated with vascular cognitive impairment.

4. The use according to claim 3, wherein the condition associated with vascular cognitive disorder is any one of non-dementia type vascular cognitive disorder, vascular cognitive disorder with sleep, adrenoleukodystrophy, acute ischemic-hypoxic encephalopathy, gut flora-shifted encephalopathy, acute ischemic stroke, acute central retinal artery occlusion.

5. The use as claimed in claim 4, wherein the Chinese medicinal composition for invigorating qi, promoting blood circulation and nourishing blood and the Chinese medicinal composition for regulating intestinal flora are used in combination in preparing the medicament for improving adrenoleukodystrophy.

6. The use of claim 5, wherein the qi-invigorating, blood-activating and blood-nourishing traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus, 6-10 parts of cassia twig, 15-30 parts of fried white peony root, 6-10 parts of medicinal cyathula root, 6-10 parts of radix achyranthis bidentatae and 6-10 parts of eucommia ulmoides; the dosage ratio of the traditional Chinese medicine composition for tonifying qi, promoting blood circulation and nourishing blood to the traditional Chinese medicine composition for regulating intestinal flora is 1: 1.

7. The use according to claim 4, wherein a lung clearing and phlegm reducing Chinese medicinal composition and the intestinal flora regulating Chinese medicinal composition are used in combination in the preparation of a medicament for improving the intestinal flora shift encephalopathy.

8. The application of claim 6, wherein the lung clearing and phlegm eliminating traditional Chinese medicine composition comprises the following components in parts by weight: 6-10 parts of cortex mori, 3-6g parts of platycodon grandiflorum, 6-10 parts of flos farfarae, 15-30 parts of gypsum, 6-10 parts of scutellaria baicalensis, 6-10 parts of prepared rhubarb and 10-15 parts of radix rehmanniae; the dosage ratio of the lung clearing and phlegm eliminating traditional Chinese medicine composition to the intestinal flora regulating traditional Chinese medicine composition is 1: 1.

9. The intestinal flora regulating traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition is prepared into any one of water decoction, granules, tablets, capsules, paste and wine.