CN111733202A - 一种泰乐菌素混种发酵方法 - Google Patents
一种泰乐菌素混种发酵方法 Download PDFInfo
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- CN111733202A CN111733202A CN202010612978.9A CN202010612978A CN111733202A CN 111733202 A CN111733202 A CN 111733202A CN 202010612978 A CN202010612978 A CN 202010612978A CN 111733202 A CN111733202 A CN 111733202A
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- fermentation
- tylosin
- diplococcus
- mixed
- culture
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Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 96
- 230000004151 fermentation Effects 0.000 title claims abstract description 96
- WBPYTXDJUQJLPQ-VMXQISHHSA-N tylosin Chemical compound O([C@@H]1[C@@H](C)O[C@H]([C@@H]([C@H]1N(C)C)O)O[C@@H]1[C@@H](C)[C@H](O)CC(=O)O[C@@H]([C@H](/C=C(\C)/C=C/C(=O)[C@H](C)C[C@@H]1CC=O)CO[C@H]1[C@@H]([C@H](OC)[C@H](O)[C@@H](C)O1)OC)CC)[C@H]1C[C@@](C)(O)[C@@H](O)[C@H](C)O1 WBPYTXDJUQJLPQ-VMXQISHHSA-N 0.000 title claims abstract description 53
- 229930194936 Tylosin Natural products 0.000 title claims abstract description 45
- 239000004182 Tylosin Substances 0.000 title claims abstract description 45
- 229960004059 tylosin Drugs 0.000 title claims abstract description 45
- 235000019375 tylosin Nutrition 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000187438 Streptomyces fradiae Species 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 29
- 239000001963 growth medium Substances 0.000 claims description 23
- 240000008042 Zea mays Species 0.000 claims description 16
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 16
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 16
- 235000005822 corn Nutrition 0.000 claims description 16
- 235000012424 soybean oil Nutrition 0.000 claims description 14
- 239000003549 soybean oil Substances 0.000 claims description 14
- 238000012807 shake-flask culturing Methods 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 241000726221 Gemma Species 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
- 230000002503 metabolic effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 238000003786 synthesis reaction Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 16
- 238000011218 seed culture Methods 0.000 description 15
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 230000001502 supplementing effect Effects 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- 238000011081 inoculation Methods 0.000 description 10
- 244000046052 Phaseolus vulgaris Species 0.000 description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 8
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 8
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 8
- 235000019838 diammonium phosphate Nutrition 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 235000016068 Berberis vulgaris Nutrition 0.000 description 6
- 241000335053 Beta vulgaris Species 0.000 description 6
- 235000019733 Fish meal Nutrition 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 6
- 239000004467 fishmeal Substances 0.000 description 6
- 235000013312 flour Nutrition 0.000 description 6
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 6
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 6
- 239000001103 potassium chloride Substances 0.000 description 6
- 235000011164 potassium chloride Nutrition 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241001052560 Thallis Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- 108010068370 Glutens Proteins 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 235000012343 cottonseed oil Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000021312 gluten Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
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CN202010612978.9A CN111733202A (zh) | 2020-06-30 | 2020-06-30 | 一种泰乐菌素混种发酵方法 |
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CN202010612978.9A CN111733202A (zh) | 2020-06-30 | 2020-06-30 | 一种泰乐菌素混种发酵方法 |
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CN111733202A true CN111733202A (zh) | 2020-10-02 |
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CN202010612978.9A Pending CN111733202A (zh) | 2020-06-30 | 2020-06-30 | 一种泰乐菌素混种发酵方法 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112779306A (zh) * | 2021-01-26 | 2021-05-11 | 浙江普洛生物科技有限公司 | 一种从发酵液中筛选碳酸钙最佳配比的方法及其用途 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020012977A1 (en) * | 2000-05-31 | 2002-01-31 | Pacey Michael Stephen | Enzymatic oxidations |
CN103074402A (zh) * | 2013-02-05 | 2013-05-01 | 宁夏泰瑞制药股份有限公司 | 弗氏链霉菌发酵生产泰乐菌素的培养基及发酵方法 |
CN103642897A (zh) * | 2013-11-18 | 2014-03-19 | 宁夏泰瑞制药股份有限公司 | 一种以脂肪酶活为指标快速筛选泰乐菌素高效价菌株及培养基的方法 |
CN103709218A (zh) * | 2013-05-22 | 2014-04-09 | 浙江普洛康裕生物制药有限公司 | 一种提高泰乐菌素a组分的提取方法 |
CN111139197A (zh) * | 2019-11-19 | 2020-05-12 | 湖北回盛生物科技有限公司 | 一种弗氏链霉菌及其在泰乐菌素发酵时的应用 |
-
2020
- 2020-06-30 CN CN202010612978.9A patent/CN111733202A/zh active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020012977A1 (en) * | 2000-05-31 | 2002-01-31 | Pacey Michael Stephen | Enzymatic oxidations |
CN103074402A (zh) * | 2013-02-05 | 2013-05-01 | 宁夏泰瑞制药股份有限公司 | 弗氏链霉菌发酵生产泰乐菌素的培养基及发酵方法 |
CN103709218A (zh) * | 2013-05-22 | 2014-04-09 | 浙江普洛康裕生物制药有限公司 | 一种提高泰乐菌素a组分的提取方法 |
CN103642897A (zh) * | 2013-11-18 | 2014-03-19 | 宁夏泰瑞制药股份有限公司 | 一种以脂肪酶活为指标快速筛选泰乐菌素高效价菌株及培养基的方法 |
CN111139197A (zh) * | 2019-11-19 | 2020-05-12 | 湖北回盛生物科技有限公司 | 一种弗氏链霉菌及其在泰乐菌素发酵时的应用 |
Non-Patent Citations (2)
Title |
---|
刘叶青,张秀萍,曹学君,郭晓敏,臧洁,王卫,刘世民,张兵: "泰乐星提取方法的研究" * |
刘恒等: "泰乐菌素的性能、药物疗效和促生长作用简介及抗生素饲料添加剂应用现状和前景浅议" * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112779306A (zh) * | 2021-01-26 | 2021-05-11 | 浙江普洛生物科技有限公司 | 一种从发酵液中筛选碳酸钙最佳配比的方法及其用途 |
CN112779306B (zh) * | 2021-01-26 | 2022-05-27 | 安徽普洛生物科技有限公司 | 一种从发酵液中筛选碳酸钙最佳配比的方法及其用途 |
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Address after: 750205 No. 1, Pacific Road, Nuanquan Industrial Park, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Applicant after: Ningxia Taiyixin Biotechnology Co.,Ltd. Address before: 750205 Yinchuan Biotechnology Park, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Applicant before: NINGXIA TAIYICIN BIOTECH Co.,Ltd. |
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Application publication date: 20201002 |
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