CN111690514A - Automatic algae bacterium isolation culture method - Google Patents
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Abstract
The invention discloses an automatic algae bacterium isolation culture method, which comprises the following steps: firstly, opening a water inlet valve to allow algae culture water to enter, enabling the algae culture water to reach an electric three-way valve through a pipeline, opening the algae culture water by the electric three-way valve to reach the control temperature of 4-35 ℃ through a water pump to a thermostat, then conveying the algae culture water into a transparent culture device from a liquid inlet pipe through a straight pipe part of an ejector, and simultaneously sucking algae seeds and culture gas mixed algae culture water into a branch pipe part of the ejector due to siphon effect to form algae seed culture water; secondly, starting a water pump to pump out algae culture water in the transparent culture device through a water return pipe to form a culture circulation; thirdly, after culturing for 4-7 days, the algae seeds meet the requirements of breeding, and the circuit control board starts the step one to the step three again to form an automatic culture circulation. The invention realizes the input of algae seeds, the continuous automatic circulation of the algae growth environment and the water body in the transparent culture device, the whole process is aseptic, the culture of algae is automatically controlled, and the sustainable large-scale commercial application is realized.
Description
Technical Field
The invention relates to the technical field of algae cultivation, in particular to an automatic algae bacteria-isolation cultivation method.
Background
At present, the basic culture mode of algae is as follows:
(I) pure culture and Mono-culture
1. Pure culture, i.e., aseptic culture, means culture under the condition that all organisms including bacteria are excluded. Pure culture requires facilities such as a sterile room, a sterilization pot, an ultra-clean workbench and the like, and containers, tools, culture solution and the like are thoroughly sterilized, so that the operation is strict, the culture success rate is high, and the method is an indispensable link for carrying out biological science research.
2. One way of culture, in which the presence of bacteria is not excluded in productive culture, as opposed to pure culture, is called monoculture.
(II) one-time culture, continuous culture and semi-continuous culture
1. Culturing in various containers at one time, preparing culture solution, inoculating a small amount of algae seeds, culturing in a proper environment, and harvesting all algae cells at one time after a period of time (generally 5-7 days) until the growth and propagation of the algae cells reach higher density. One-time culture is the most common method for culturing microalgae.
2. Semi-continuous culture is that on the basis of one-time culture, when the cultured algae cells reach or approach the harvest density, a part of algae liquid is harvested every day and supplemented with the same amount of culture liquid, and the culture is continued. Semi-continuous culture is also a commonly used culture method for microalgae bait productivity, and the yield per day is determined according to the requirement of seedling culture.
As is apparent from the above background art, there is no apparatus or method capable of automatically cultivating algae, and the inventors of the present invention have conducted extensive studies on the above problems, and how to realize the automatic algae-removal cultivation is an object of research by those skilled in the art.
Disclosure of Invention
The invention provides an automatic algae bacterium isolation culture method which is large in single culture quantity of algae, high in survival rate and high in large-scale commercial culture value.
An automatic algae bacteria-isolation culture method comprises the following steps:
firstly, opening a water inlet valve to feed algae culture water, enabling the algae culture water to reach an electric three-way valve through a pipeline, opening the algae culture water by the electric three-way valve, enabling the algae culture water to reach the control temperature of 4-35 ℃ through a water pump, then conveying the algae culture water into a transparent culture device from a liquid inlet pipe (30) through a straight pipe part of an ejector, simultaneously sucking algae seeds and culture gas mixed algae culture water through a branch pipe part of the ejector due to siphon effect to form algae seed culture water, enabling the algae seed culture water to continuously enter the transparent culture device, automatically opening an exhaust valve on the outer side of the transparent culture device, sensing the water level by a water level sensor, and controlling the water inlet valve to be closed through a circuit control board after the algae;
secondly, starting a water pump to pump out algae seed culture water in the transparent culture device through a water return pipe (29), automatically opening a vacuum valve to prevent negative pressure, sensing the temperature and the PH value through a temperature sensor, adjusting the temperature through a thermostat, sucking algae seeds and culture gas by an ejector to increase the activity of the algae seeds, and then entering the transparent culture device to form a culture cycle;
thirdly, after culturing for 4-7 days, the algae seeds meet the requirements for breeding, and the electromagnetic valve is started to enable the algae seed culture water which is cultured to flow out of the algae discharging faucet through the flowmeter and the electromagnetic valve;
fourthly, after the cultivation is finished, the circuit control board forms an automatic cultivation cycle from the step one to the step three of the new starting.
The transparent culture device comprises more than two mutually connected transparent pipe sections, and the more than two mutually connected transparent pipe sections are fastened and connected through flange plates and bolts.
The outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor; the outer side of the water return pipe is connected with a temperature sensor; and the water level sensor and the temperature sensor are both connected with the circuit control board.
Transparent culture apparatus in be equipped with integrated algae species input, circulation culture, cleaning system, algae species input, circulation culture, cleaning system including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located the inside algae species input tube of transparent culture apparatus, algae species input tube export is located transparent culture apparatus border, algae species input tube outer wall is equipped with the washing nozzle.
The algae seed input tube rotary driving device comprises a fixed connecting tube connected with one end of a liquid inlet tube, the other end of the fixed connecting tube is connected with a bearing in an adapter, another independent bearing is arranged in the adapter, the independent bearing is connected with one end of a tubular rotary joint with a key pin surface, the periphery of the tubular rotary joint is connected with a driven gear, the driven gear is meshed with a driving gear on a motor, the other end of the tubular rotary joint is connected with a tubular driving joint, and the other end of the tubular driving joint is connected with an algae seed input tube.
The periphery of the tubular driving joint is respectively sleeved with a first flange plate, a fixed sealing ring, a rotary sealing ring and a second flange plate, and the first flange plate and the second flange plate are clamped and connected with the outer wall of the transparent culture device through screws; the periphery of the rotary sealing ring is sleeved with a spring.
A plane bearing is arranged between the first flange plate and the fixed sealing ring, and a first plane bearing is arranged between the rotary sealing ring and the second flange plate.
And metal sealing rings are arranged in the fixed sealing ring and the rotary sealing ring, and the two metal sealing rings are mutually attached and sealed.
The sprayer is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with the thermostat and the liquid inlet pipe, the branch pipe part of the three-way pipe structure is connected with the spray joint, the inside of the spray joint is provided with the sealing ring, the inner hole of the sealing ring is hermetically matched with the sealing ball, and the end part of the sealing ball is connected with the sealing spring arranged inside the branch pipe.
A PH value sensor is connected on a pipeline between the thermostat and the water pump; and a flow meter, an electromagnetic valve and an algae discharging tap are connected between the thermostat and the ejector through pipelines.
The periphery of the algae input pipe is connected with a supporting bearing, the periphery of the supporting bearing is connected with more than three supporting rods which are uniformly distributed, the end parts of the supporting rods are connected with supporting ejector rods through adjusting springs, and the supporting ejector rods are abutted to the inner wall of the transparent pipe section.
From the above embodiments, it is apparent that the present invention has the following advantages:
1. the invention realizes the input of algae seeds, the continuous automatic circulation of the algae growth environment and the water body in the transparent culture device, the whole process is aseptic, the culture of algae is automatically controlled, and the sustainable large-scale commercial application is realized.
2. The invention greatly increases the amount of algae cultivated in a single time by more than two transparent pipe sections which are mutually connected, and improves the economic value of algae cultivation.
3. The invention solves the problems of automatic supply and circulating culture of algae and cleaning of algae attached to the pipe wall of the transparent culture device after long-term culture by arranging the integrated algae seed input, circulating culture and cleaning system in the transparent culture device.
Drawings
FIG. 1 is a schematic diagram of the principle structure of the automated bacteria-isolated algae cultivation method of the present invention;
FIG. 2 is a schematic view of the exploded perspective structure of the algae seed input tube rotation driving device according to the present invention;
fig. 3 is a schematic exploded perspective view of the injector of the present invention.
Detailed Description
An automatic algae bacteria-isolation cultivation method as shown in fig. 1-3, the automatic algae bacteria-isolation cultivation method comprises the following steps:
firstly, opening a water inlet valve to feed algae culture water, enabling the algae culture water to reach an electric three-way valve through a pipeline, opening the algae culture water by the electric three-way valve, enabling the algae culture water to reach the control temperature of 4-35 ℃ through a water pump, then conveying the algae culture water into a transparent culture device from a liquid inlet pipe 30 through a straight pipe part of an ejector, simultaneously sucking algae seeds and culture gas mixed algae culture water through a branch pipe part of the ejector due to siphon effect to form algae seed culture water, enabling the algae seed culture water to continuously enter the transparent culture device, automatically opening an exhaust valve at the outer side of the transparent culture device, sensing the water level by a water level sensor, and controlling the water inlet valve to be closed through a circuit control board after the algae;
secondly, starting a water pump to pump out algae seed culture water in the transparent culture device through a water return pipe 29, automatically opening a vacuum valve to prevent negative pressure, sensing the temperature and the PH value through a temperature sensor, adjusting the temperature through a constant temperature machine, further sucking algae seeds and culture gas by an ejector to increase the activity of the algae seeds, and then entering the transparent culture device to form a culture cycle; after the PH value is detected, if the PH value is not in accordance with the requirement, the water can be discharged from the water drain valve to avoid polluting the culture water body.
Thirdly, after culturing for 4-7 days, the algae seeds meet the requirements for breeding, and the electromagnetic valve is started to enable the algae seed culture water which is cultured to flow out of the algae discharging faucet through the flowmeter and the electromagnetic valve;
fourthly, after the cultivation is finished, the circuit control board forms an automatic cultivation cycle from the step one to the step three of the new starting.
The water inlet valve, the electric three-way valve, the water pump, the thermostat and the ejector which are communicated by pipelines are all algae cultivation control devices through a liquid inlet pipe 30. The algae cultivation control apparatus is entirely located in the control box 22.
The ejector 23 is preferably connected with a gas-algae input device, the gas-algae input device comprises an algae species input device and a gas input device, and the algae species input device comprises an algae species input interface and a first check valve which are connected through pipelines to realize the one-way input performance of algae species; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines, the one-way input performance of the culture gas after sterilization treatment is realized, the algae seeds are mixed with the gas and then are merged into the culture environment in the transparent culture device, the oxygen content of the water body is continuously increased, the culture speed of the algae seeds is improved, and the death of the algae is avoided. As the air pressure automatic adjusting structure of the transparent culture device, the outer side of the transparent culture device is respectively connected with the exhaust valve, the vacuum valve and the water level sensor, the exhaust valve exhausts air when the transparent culture device is filled with water, the vacuum valve is automatically opened to avoid negative pressure when algae is placed after culture is completed, and algae cannot be placed, and the water level sensor controls the water level of the transparent culture device in real time to supplement water. The water entering from the water inlet valve is filtered by the filter, and the filter is connected with the drain valve.
The transparent culture device is connected with an electric three-way valve through a water return pipe 29, and then the liquid in the transparent culture device is output by the driving of a water pump to realize the internal circulation of algae culture water. The outer side of the water return pipe 29 is connected with a temperature sensor; and the water level sensor and the temperature sensor are both connected with the circuit control board.
The transparent culture device preferably comprises more than two mutually connected transparent pipe sections 3, and the more than two mutually connected transparent pipe sections 3 are fixedly connected through the flange plates 4 and the bolts 9, so that the capacity of the transparent culture device is increased, and water leakage is avoided. Be equipped with integrated algae species input, circulation culture, cleaning system in the transparent culture apparatus, algae species input, circulation culture, cleaning system are including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located the inside algae species input tube 1 of transparent culture apparatus, the export of 1 kind of algae species input tube is located transparent culture apparatus border, 1 play liquid of algae species input tube drives whole transparent culture apparatus in the liquid flow prevent that the algae from deposiing, be favorable to the cultivation of algae more.
The algae species input pipe rotary driving device 8 with the optimized structure comprises a fixed connecting pipe 18 connected with one end of a liquid inlet pipe 30, the other end of the fixed connecting pipe 18 is connected with a bearing in an adapter 17, another independent bearing is arranged in the adapter 17 and is connected with one end of a tubular rotary joint 16 with a key pin surface 19, the periphery of the tubular rotary joint 16 is connected with a driven gear 6, the driven gear 6 is meshed with a driving gear on a motor 7, the other end of the tubular rotary joint 16 is connected with a tubular driving joint 31, the other end of the tubular driving joint 31 is connected with an algae species input pipe 1, and the outer wall of the algae species input pipe 1 is provided with a cleaning nozzle 2. The algae seed input pipe rotation driving device 8 realizes the rotation performance of the algae seed input pipe 1, the output liquid has rotation flow velocity to further prevent algae precipitation, and the cleaning nozzle 2 utilizes the high pressure of the nozzle to achieve the cleaning performance of the transparent culture device to prevent algae adhesion. The washing nozzle 2 may be an outlet for the algae seed input liquid.
As the peripheral support bearing 34 that is connected with of the preferred algae kind input tube 1 of structure, support bearing 34 peripheral is connected with the bracing piece 33 of three above evenly distributed, and the bracing piece 33 tip is connected with support ejector pin 31 through adjusting spring 32, and support ejector pin 31 butt joint transparent pipe section 3 inner wall, this kind of bearing structure is through adjusting spring 32's elastic deformation for interconnect's algae kind input tube 1 can not appear crooked problem because of the overlength, keeps the equilibrium performance of rotation state.
In order to further improve the connection sealing performance, the periphery of the tubular driving joint 31 is respectively sleeved with a first flange 21, a fixed sealing ring 15, a rotary sealing ring 21 and a second flange 11, and the first flange 21 and the second flange 11 are clamped and connected with the outer wall 10 of the transparent culture device through screws. In order to further prevent the transparent culture apparatus outer wall 10 from being sandwiched and loosened between the first flange 21 and the second flange 11, a spring 13 is fitted around the outer periphery of the rotary seal ring 21.
Preferably, a flat bearing is provided between the first flange 21 and the stationary seal ring 15, and a first flat bearing 12 is provided between the rotary seal ring 21 and the second flange 11, so as to reduce the frictional resistance of the tubular drive coupling 31 during rotation. The metal sealing rings 14 are arranged in the fixed sealing ring 15 and the rotary sealing ring 21, and the two metal sealing rings 14 are mutually attached and sealed, so that the sealing effect of the whole machine surface and the sealing surface is improved, and water leakage is prevented. The axial cross section of the rotary sealing ring 21 is of a circular arc structure, and when the rotary sealing ring 21 is compressed, the inner wall can generate a pressing sealing force on the outer wall of the tubular driving joint 31, so that the sealing effect is further improved.
In order to form a siphon effect, the ejector 23 is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with a thermostat and a liquid inlet pipe 30, the branch pipe part of the three-way pipe structure is connected with an injection joint 28, a sealing ring 26 is arranged inside the injection joint 28, an inner hole of the sealing ring 26 is hermetically matched with a sealing ball 25, the end part of the sealing ball 25 is connected with a sealing spring 24 arranged inside the branch pipe to realize one-way sealing, the inner diameter of the straight pipe body is reduced in the liquid flowing process in the straight line part of the three-way pipe structure, and then the inner diameter of the straight.
As a structure preference, a PH value sensor is connected on a pipeline between the thermostat and the water pump to control the water inflow of the water inlet valve; a flowmeter, an electromagnetic valve and an algae discharging tap are connected between the constant temperature machine and the ejector through pipelines, so that the cultured algae can be automatically discharged.
Claims (8)
1. An automatic alga bacteria-isolation culture method is characterized in that: the automatic algae bacterium isolation culture method comprises the following steps:
the water inlet valve is opened, algae culture water enters the electric three-way valve through a pipeline, the electric three-way valve opens the algae culture water, the temperature of the algae culture water is controlled to be between 4 and 35 ℃ through the water pump, then the algae culture water is conveyed into the transparent culture device through the liquid inlet pipe (30) through the straight pipe part of the ejector, meanwhile, algae seeds and culture gas mixed algae culture water is sucked into the branch pipe part of the ejector due to the siphon effect to form algae seed culture water, the algae seed culture water continuously enters the transparent culture device, the exhaust valve on the outer side of the transparent culture device is automatically opened, the water level sensor senses the water level, and after the water level reaches the set water level, the water inlet valve is controlled to be closed through;
the water pump is started to pump out algae seed culture water in the transparent culture device through the water return pipe (29), the vacuum valve is automatically opened to prevent negative pressure, the temperature and the PH value are sensed through the temperature sensor and then adjusted through the thermostat, algae seeds and culture gas are sucked through the ejector to increase the activity of the algae seeds, and then the algae seeds enter the transparent culture device to form a culture circulation;
after culturing for 4-7 days, the algae breeding reaches the requirement, and the electromagnetic valve is started to make the algae culture water flow out from the algae discharging tap through the flowmeter and the electromagnetic valve;
after the cultivation is finished, the circuit control board forms an automatic cultivation cycle from the step one to the step three by starting.
2. The automated method of claim 1, wherein: the transparent culture device comprises more than two mutually connected transparent pipe sections (3), and the more than two mutually connected transparent pipe sections (3) are fixedly connected through flange plates (4) and matched bolts (9).
3. The automated method of claim 1, wherein: transparent culture apparatus in be equipped with integrated algae species input, circulation culture, cleaning system, algae species input, circulation culture, cleaning system including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located inside algae species input tube (1) of transparent culture apparatus, algae species input tube (1) export is located transparent culture apparatus border, algae species input tube (1) outer wall is equipped with cleaning nozzle (2).
4. An automated method of claim 3, wherein the method comprises: algae kind input tube rotary driving device (8) including fixed connection pipe (18) of connecting feed liquor pipe (30) one end, a bearing in fixed connection pipe (18) other end connection crossover sub (17), be equipped with another independent bearing in crossover sub (17), tubulose rotary joint (16) one end that has key round pin face (19) is connected to independent bearing, tubulose rotary joint (16) periphery is connected with driven gear (6), driving gear on motor (7) is connected in driven gear (6) meshing, tubulose rotary joint (31) are connected to tubulose rotary joint (16) other end, algae kind input tube (1) is connected to tubulose drive joint (31) other end.
5. An automated method of claim 4, wherein the method comprises: the periphery of the tubular driving joint (31) is respectively sleeved with a first flange plate (21), a fixed sealing ring (15), a rotary sealing ring (21) and a second flange plate (11), and the first flange plate (21) and the second flange plate (11) are clamped and connected with the outer wall of the transparent culture device through screws; the periphery of the rotary sealing ring (21) is sleeved with a spring (13).
6. An automated method of claim 5, wherein the method comprises: a plane bearing is arranged between the first flange plate (21) and the fixed sealing ring (15), and a first plane bearing (12) is arranged between the rotary sealing ring (21) and the second flange plate (11).
7. An automated method of claim 6, wherein the method comprises: and the metal sealing rings (14) are arranged in the fixed sealing ring (15) and the rotary sealing ring (21), and the two metal sealing rings (14) are mutually attached and sealed.
8. An automated method of claim 4, wherein the method comprises: the periphery of the algae seed input pipe (1) is connected with a supporting bearing (34), the periphery of the supporting bearing (34) is connected with more than three supporting rods (33) which are uniformly distributed, the end parts of the supporting rods (33) are connected with supporting ejector rods (31) through adjusting springs (32), and the supporting ejector rods (31) are connected with the inner wall of the transparent pipe section (3) in a jacking mode.
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| CN202010648097.2A CN111690514B (en) | 2020-07-07 | 2020-07-07 | Automatic algae bacteria-isolation culture method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2914315A1 (en) * | 2007-03-29 | 2008-10-03 | Chao-Hui Lu | Photosynthesis of algae e.g. Spirulina, comprises injecting liquid culture and algae spores in transparent pipe, operating pressurized fluid transport unit to force liquid culture to flow towards oxygen jet, and collecting liquid from jet |
| US20110124091A1 (en) * | 2009-11-24 | 2011-05-26 | Chao-Hui Lu | Industrialized algae culturing method and system thereof |
| CN102533528A (en) * | 2012-01-12 | 2012-07-04 | 天津大学 | Sealed continuous culture experimental apparatus based on simulation of microalgae amplification culture |
| CN104560695A (en) * | 2015-01-21 | 2015-04-29 | 北京化工大学 | Tubular photo-bioreactor based on built-in fluid driven rotor |
| CN105733930A (en) * | 2016-05-05 | 2016-07-06 | 中国科学院广州能源研究所 | Rotating disc type photobioreactor for microalgae large-scale culture |
| CN206705760U (en) * | 2016-10-17 | 2017-12-05 | 成都川力智能流体设备股份有限公司 | A kind of pipeline Antimicrobial device |
| CN108018194A (en) * | 2018-01-12 | 2018-05-11 | 华南理工大学 | A kind of duct type bioreactor for microalgae mass culture |
| KR101996310B1 (en) * | 2018-12-20 | 2019-07-04 | 최정환 | Chlorella culture device |
| CN110760439A (en) * | 2019-11-08 | 2020-02-07 | 深圳市德和生物科技有限公司 | Algae cultivation photo-biological reaction kettle and continuous culture reaction system containing same |
-
2020
- 2020-07-07 CN CN202010648097.2A patent/CN111690514B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2914315A1 (en) * | 2007-03-29 | 2008-10-03 | Chao-Hui Lu | Photosynthesis of algae e.g. Spirulina, comprises injecting liquid culture and algae spores in transparent pipe, operating pressurized fluid transport unit to force liquid culture to flow towards oxygen jet, and collecting liquid from jet |
| US20110124091A1 (en) * | 2009-11-24 | 2011-05-26 | Chao-Hui Lu | Industrialized algae culturing method and system thereof |
| CN102533528A (en) * | 2012-01-12 | 2012-07-04 | 天津大学 | Sealed continuous culture experimental apparatus based on simulation of microalgae amplification culture |
| CN104560695A (en) * | 2015-01-21 | 2015-04-29 | 北京化工大学 | Tubular photo-bioreactor based on built-in fluid driven rotor |
| CN105733930A (en) * | 2016-05-05 | 2016-07-06 | 中国科学院广州能源研究所 | Rotating disc type photobioreactor for microalgae large-scale culture |
| CN206705760U (en) * | 2016-10-17 | 2017-12-05 | 成都川力智能流体设备股份有限公司 | A kind of pipeline Antimicrobial device |
| CN108018194A (en) * | 2018-01-12 | 2018-05-11 | 华南理工大学 | A kind of duct type bioreactor for microalgae mass culture |
| KR101996310B1 (en) * | 2018-12-20 | 2019-07-04 | 최정환 | Chlorella culture device |
| CN110760439A (en) * | 2019-11-08 | 2020-02-07 | 深圳市德和生物科技有限公司 | Algae cultivation photo-biological reaction kettle and continuous culture reaction system containing same |
Non-Patent Citations (2)
| Title |
|---|
| 孙林枫: "藻类连续培养及其应用", 海洋环境科学, no. 02, pages 65 - 70 * |
| 白志毅等: "循环水产养殖***生物安全体系的构件", 《渔业现代化》, no. 2, pages 1 - 4 * |
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