CN111718852A - Automatic change alga and separate fungus culture apparatus - Google Patents

Automatic change alga and separate fungus culture apparatus Download PDF

Info

Publication number
CN111718852A
CN111718852A CN202010649008.6A CN202010649008A CN111718852A CN 111718852 A CN111718852 A CN 111718852A CN 202010649008 A CN202010649008 A CN 202010649008A CN 111718852 A CN111718852 A CN 111718852A
Authority
CN
China
Prior art keywords
algae
culture
transparent
pipe
sealing ring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202010649008.6A
Other languages
Chinese (zh)
Other versions
CN111718852B (en
Inventor
傅百成
白志毅
袁屹平
刘曙光
傅煜茖
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Bairuila Agricultural Technology Co ltd
Shanghai Ocean University
Original Assignee
Zhejiang Bairuila Agricultural Technology Co ltd
Shanghai Ocean University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Bairuila Agricultural Technology Co ltd, Shanghai Ocean University filed Critical Zhejiang Bairuila Agricultural Technology Co ltd
Priority to CN202010649008.6A priority Critical patent/CN111718852B/en
Publication of CN111718852A publication Critical patent/CN111718852A/en
Application granted granted Critical
Publication of CN111718852B publication Critical patent/CN111718852B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/16—Screw conveyor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses an automatic algae bacteria-isolation culture device, which comprises an algae culture control device and a transparent culture device, wherein the transparent culture device is arranged on the algae culture control device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a constant temperature machine and an ejector which are communicated through pipelines, and the ejector is connected with the transparent culture device through a liquid inlet pipe; the gas-algae input device comprises an algae species input device and a gas input device, and the algae species input device comprises an algae species input interface and a first check valve which are connected through a pipeline; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with an electric three-way valve through a water return pipe. The invention adopts the algae culture control device to realize the input of algae seeds and the continuous automatic circulation of the algae growth environment and the water body in the transparent culture device, and the whole course is aseptic, the culture of algae is automatically controlled, and the sustainable large-scale commercial application is realized.

Description

Automatic change alga and separate fungus culture apparatus
Technical Field
The invention relates to the technical field of algae cultivation, in particular to an automatic algae bacteria-isolation cultivation device.
Background
At present, the basic culture mode of algae is as follows:
(I) pure culture and Mono-culture
1. Pure culture, i.e., aseptic culture, means culture under the condition that all organisms including bacteria are excluded. Pure culture requires facilities such as a sterile room, a sterilization pot, an ultra-clean workbench and the like, and containers, tools, culture solution and the like are thoroughly sterilized, so that the operation is strict, the culture success rate is high, and the method is an indispensable link for carrying out biological science research.
2. One way of culture, in which the presence of bacteria is not excluded in productive culture, as opposed to pure culture, is called monoculture.
(II) one-time culture, continuous culture and semi-continuous culture
1. Culturing in various containers at one time, preparing culture solution, inoculating a small amount of algae seeds, culturing in a proper environment, and harvesting all algae cells at one time after a period of time (generally 5-7 days) until the growth and propagation of the algae cells reach higher density. One-time culture is the most common method for culturing microalgae.
2. Semi-continuous culture is that on the basis of one-time culture, when the cultured algae cells reach or approach the harvest density, a part of algae liquid is harvested every day and supplemented with the same amount of culture liquid, and the culture is continued. Semi-continuous culture is also a commonly used culture method for microalgae bait productivity, and the yield per day is determined according to the requirement of seedling culture.
As is apparent from the above background art, there is no apparatus or method capable of automatically cultivating algae, and the inventors of the present invention have conducted extensive studies on the above problems, and how to realize the automatic algae-removal cultivation is an object of research by those skilled in the art.
Disclosure of Invention
The invention provides an automatic algae bacterium isolation culture device which is large in single-time culture quantity of algae, high in survival rate and high in large-scale commercial culture value.
An automatic algae bacteria-isolating culture device comprises an algae culture control device and a transparent culture device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a constant temperature machine and an ejector which are communicated through pipelines, and the ejector is connected with the transparent culture device through a liquid inlet pipe; the gas-algae input device comprises an algae species input device and a gas input device, and the algae species input device comprises an algae species input interface and a first check valve which are connected through a pipeline; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with an electric three-way valve through a water return pipe.
The transparent culture device comprises more than two mutually connected transparent pipe sections, and the more than two mutually connected transparent pipe sections are fastened and connected through flange plates and bolts.
The outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor; the outer side of the water return pipe is connected with a temperature sensor; and the water level sensor and the temperature sensor are both connected with the circuit control board.
Transparent culture apparatus in be equipped with integrated algae species input, circulation culture, cleaning system, algae species input, circulation culture, cleaning system including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located the inside algae species input tube of transparent culture apparatus, algae species input tube export is located transparent culture apparatus border, algae species input tube outer wall is equipped with the washing nozzle.
The algae seed input tube rotary driving device comprises a fixed connecting tube connected with one end of a liquid inlet tube, the other end of the fixed connecting tube is connected with a bearing in an adapter, another independent bearing is arranged in the adapter, the independent bearing is connected with one end of a tubular rotary joint with a key pin surface, the periphery of the tubular rotary joint is connected with a driven gear, the driven gear is meshed with a driving gear on a motor, the other end of the tubular rotary joint is connected with a tubular driving joint, and the other end of the tubular driving joint is connected with an algae seed input tube.
The periphery of the tubular driving joint is respectively sleeved with a first flange plate, a fixed sealing ring, a rotary sealing ring and a second flange plate, and the first flange plate and the second flange plate are clamped and connected with the outer wall of the transparent culture device through screws; the periphery of the rotary sealing ring is sleeved with a spring.
A plane bearing is arranged between the first flange plate and the fixed sealing ring, and a first plane bearing is arranged between the rotary sealing ring and the second flange plate.
And metal sealing rings are arranged in the fixed sealing ring and the rotary sealing ring, and the two metal sealing rings are mutually attached and sealed.
The sprayer is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with the thermostat and the liquid inlet pipe, the branch pipe part of the three-way pipe structure is connected with the spray joint, the inside of the spray joint is provided with the sealing ring, the inner hole of the sealing ring is hermetically matched with the sealing ball, and the end part of the sealing ball is connected with the sealing spring arranged inside the branch pipe.
A PH value sensor is connected on a pipeline between the thermostat and the water pump; and a flow meter, an electromagnetic valve and an algae discharging tap are connected between the thermostat and the ejector through pipelines.
The periphery of the algae input pipe is connected with a supporting bearing, the periphery of the supporting bearing is connected with more than three supporting rods which are uniformly distributed, the end parts of the supporting rods are connected with supporting ejector rods through adjusting springs, and the supporting ejector rods are abutted to the inner wall of the transparent pipe section.
From the above embodiments, it is apparent that the present invention has the following advantages:
1. the invention adopts the algae culture control device to realize the input of algae seeds and the continuous automatic circulation of the algae growth environment and the water body in the transparent culture device, and the whole course is aseptic, the culture of algae is automatically controlled, and the sustainable large-scale commercial application is realized.
2. The invention greatly increases the amount of algae cultivated in a single time by more than two transparent pipe sections which are mutually connected, and improves the economic value of algae cultivation.
3. The invention solves the problems of automatic supply and circulating culture of algae and cleaning of algae attached to the pipe wall of the transparent culture device after long-term culture by arranging the integrated algae seed input, circulating culture and cleaning system in the transparent culture device.
Drawings
FIG. 1 is a schematic structural diagram of an automated algae bacteria-isolating culture apparatus according to the present invention;
FIG. 2 is a schematic view of the exploded perspective structure of the algae seed input tube rotation driving device according to the present invention;
fig. 3 is a schematic exploded perspective view of the injector of the present invention.
Detailed Description
As shown in fig. 1-3, an automatic algae bacteria-isolating cultivation device comprises an algae cultivation control device and a transparent cultivation device, wherein the algae cultivation control device comprises a water inlet valve, an electric three-way valve, a water pump, a constant temperature machine and an ejector which are communicated through pipelines, and the ejector is connected with the transparent cultivation device through a liquid inlet pipe 30. The algae cultivation control apparatus is entirely located in the control box 22.
The ejector 23 is preferably connected with a gas-algae input device, the gas-algae input device comprises an algae species input device and a gas input device, and the algae species input device comprises an algae species input interface and a first check valve which are connected through pipelines to realize the one-way input performance of algae species; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines, the one-way input performance of the culture gas after sterilization treatment is realized, the algae seeds are mixed with the gas and then are merged into the culture environment in the transparent culture device, the oxygen content of the water body is continuously increased, the culture speed of the algae seeds is improved, and the death of the algae is avoided. As the air pressure automatic adjusting structure of the transparent culture device, the outer side of the transparent culture device is respectively connected with the exhaust valve, the vacuum valve and the water level sensor, the exhaust valve exhausts air when the transparent culture device is filled with water, the vacuum valve is automatically opened to avoid negative pressure when algae is placed after culture is completed, and algae cannot be placed, and the water level sensor controls the water level of the transparent culture device in real time to supplement water. The water entering from the water inlet valve is filtered by the filter, and the filter is connected with the drain valve.
The transparent culture device is connected with an electric three-way valve through a water return pipe 29, and then the liquid in the transparent culture device is output by the driving of a water pump to realize the internal circulation of algae culture water. The outer side of the water return pipe 29 is connected with a temperature sensor; and the water level sensor and the temperature sensor are both connected with the circuit control board.
The transparent culture device preferably comprises more than two mutually connected transparent pipe sections 3, and the more than two mutually connected transparent pipe sections 3 are fixedly connected through the flange plates 4 and the bolts 9, so that the capacity of the transparent culture device is increased, and water leakage is avoided. Be equipped with integrated algae species input, circulation culture, cleaning system in the transparent culture apparatus, algae species input, circulation culture, cleaning system are including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located the inside algae species input tube 1 of transparent culture apparatus, the export of 1 kind of algae species input tube is located transparent culture apparatus border, 1 play liquid of algae species input tube drives whole transparent culture apparatus in the liquid flow prevent that the algae from deposiing, be favorable to the cultivation of algae more.
The algae species input pipe rotary driving device 8 with the optimized structure comprises a fixed connecting pipe 18 connected with one end of a liquid inlet pipe 30, the other end of the fixed connecting pipe 18 is connected with a bearing in an adapter 17, another independent bearing is arranged in the adapter 17 and is connected with one end of a tubular rotary joint 16 with a key pin surface 19, the periphery of the tubular rotary joint 16 is connected with a driven gear 6, the driven gear 6 is meshed with a driving gear on a motor 7, the other end of the tubular rotary joint 16 is connected with a tubular driving joint 31, the other end of the tubular driving joint 31 is connected with an algae species input pipe 1, and the outer wall of the algae species input pipe 1 is provided with a cleaning nozzle 2. The algae seed input pipe rotation driving device 8 realizes the rotation performance of the algae seed input pipe 1, the output liquid has rotation flow rate to further prevent algae precipitation, and the cleaning nozzle 2 achieves the cleaning performance of the transparent culture device by utilizing high pressure of the nozzle to prevent algae adhesion and can also be used as an output port of algae seed liquid.
As the peripheral support bearing 34 that is connected with of the preferred algae kind input tube 1 of structure, support bearing 34 peripheral is connected with the bracing piece 33 of three above evenly distributed, and the bracing piece 33 tip is connected with support ejector pin 31 through adjusting spring 32, and support ejector pin 31 butt joint transparent pipe section 3 inner wall, this kind of bearing structure is through adjusting spring 32's elastic deformation for interconnect's algae kind input tube 1 can not appear crooked problem because of the overlength, keeps the equilibrium performance of rotation state.
In order to further improve the connection sealing performance, the periphery of the tubular driving joint 31 is respectively sleeved with a first flange 21, a fixed sealing ring 15, a rotary sealing ring 21 and a second flange 11, and the first flange 21 and the second flange 11 are clamped and connected with the outer wall 10 of the transparent culture device through screws. In order to further prevent the transparent culture apparatus outer wall 10 from being sandwiched and loosened between the first flange 21 and the second flange 11, a spring 13 is fitted around the outer periphery of the rotary seal ring 21.
Preferably, a flat bearing is provided between the first flange 21 and the stationary seal ring 15, and a first flat bearing 12 is provided between the rotary seal ring 21 and the second flange 11, so as to reduce the frictional resistance of the tubular drive coupling 31 during rotation. The metal sealing rings 14 are arranged in the fixed sealing ring 15 and the rotary sealing ring 21, and the two metal sealing rings 14 are mutually attached and sealed, so that the sealing effect of the whole machine surface and the sealing surface is improved, and water leakage is prevented. The axial cross section of the rotary sealing ring 21 is of a circular arc structure, and when the rotary sealing ring 21 is compressed, the inner wall can generate a pressing sealing force on the outer wall of the tubular driving joint 31, so that the sealing effect is further improved.
In order to form a siphon effect, the ejector 23 is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with a thermostat and a liquid inlet pipe 30, the branch pipe part of the three-way pipe structure is connected with an injection joint 28, a sealing ring 26 is arranged inside the injection joint 28, an inner hole of the sealing ring 26 is hermetically matched with a sealing ball 25, the end part of the sealing ball 25 is connected with a sealing spring 24 arranged inside the branch pipe to realize one-way sealing, the inner diameter of the straight pipe body is reduced in the liquid flowing process in the straight line part of the three-way pipe structure, and then the inner diameter of the straight.
As a structure preference, a PH value sensor is connected on a pipeline between the thermostat and the water pump to control the water inflow of the water inlet valve; a flowmeter, an electromagnetic valve and an algae discharging tap are connected between the constant temperature machine and the ejector through pipelines, so that the cultured algae can be automatically discharged.

Claims (10)

1. The utility model provides an automatic change alga and separate fungus culture apparatus which characterized in that: the automatic algae bacterium-isolating culture device comprises an algae culture control device and a transparent culture device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a constant temperature machine and an ejector which are communicated through pipelines, and the ejector is connected with the transparent culture device through a liquid inlet pipe (30); the gas-algae input device comprises an algae species input device and a gas input device, and the algae species input device comprises an algae species input interface and a first check valve which are connected through a pipeline; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with an electric three-way valve through a water return pipe (29).
2. The automated algae removal and cultivation apparatus of claim 1, wherein: the transparent culture device comprises more than two mutually connected transparent pipe sections (3), and the more than two mutually connected transparent pipe sections (3) are fixedly connected through flange plates (4) and matched bolts (9).
3. The automated algae removal and cultivation apparatus of claim 1, wherein: the outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor; the outer side of the water return pipe (29) is connected with a temperature sensor; and the water level sensor and the temperature sensor are both connected with the circuit control board.
4. The automated algae removal and cultivation apparatus of claim 1, wherein: transparent culture apparatus in be equipped with integrated algae species input, circulation culture, cleaning system, algae species input, circulation culture, cleaning system including the algae species input tube rotary driving device who is located the transparent culture apparatus outside to and be located inside algae species input tube (1) of transparent culture apparatus, algae species input tube (1) export is located transparent culture apparatus border, algae species input tube (1) outer wall is equipped with cleaning nozzle (2).
5. An automated algae removal apparatus according to claim 4, wherein: algae kind input tube rotary driving device (8) including fixed connection pipe (18) of connecting feed liquor pipe (30) one end, a bearing in fixed connection pipe (18) other end connection crossover sub (17), be equipped with another independent bearing in crossover sub (17), tubulose rotary joint (16) one end that has key round pin face (19) is connected to independent bearing, tubulose rotary joint (16) periphery is connected with driven gear (6), driving gear on motor (7) is connected in driven gear (6) meshing, tubulose rotary joint (31) are connected to tubulose rotary joint (16) other end, algae kind input tube (1) is connected to tubulose drive joint (31) other end.
6. An automated algae removal apparatus according to claim 5, wherein: the periphery of the tubular driving joint (31) is respectively sleeved with a first flange plate (21), a fixed sealing ring (15), a rotary sealing ring (21) and a second flange plate (11), and the first flange plate (21) and the second flange plate (11) are clamped and connected with the outer wall of the transparent culture device through screws; the periphery of the rotary sealing ring (21) is sleeved with a spring (13).
7. An automated algae removal apparatus according to claim 6, wherein: a plane bearing is arranged between the first flange plate (21) and the fixed sealing ring (15), and a first plane bearing (12) is arranged between the rotary sealing ring (21) and the second flange plate (11).
8. An automated algae removal apparatus according to claim 6, wherein: and the metal sealing rings (14) are arranged in the fixed sealing ring (15) and the rotary sealing ring (21), and the two metal sealing rings (14) are mutually attached and sealed.
9. The automated algae removal and cultivation apparatus of claim 1, wherein: the sprayer (23) is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with a thermostat and a liquid inlet pipe (30), the branch pipe part of the three-way pipe structure is connected with an injection joint (28), a sealing ring (26) is arranged inside the injection joint (28), an inner hole of the sealing ring (26) is hermetically matched with a sealing ball (25), and the end part of the sealing ball (25) is connected with a sealing spring (24) arranged inside the branch pipe.
10. An automated algae removal apparatus according to claim 4, wherein: the periphery of the algae seed input pipe (1) is connected with a supporting bearing (34), the periphery of the supporting bearing (34) is connected with more than three supporting rods (33) which are uniformly distributed, the end parts of the supporting rods (33) are connected with supporting ejector rods (31) through adjusting springs (32), and the supporting ejector rods (31) are connected with the inner wall of the transparent pipe section (3) in a jacking mode.
CN202010649008.6A 2020-07-07 2020-07-07 Automatic change algae and separate fungus culture apparatus Active CN111718852B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010649008.6A CN111718852B (en) 2020-07-07 2020-07-07 Automatic change algae and separate fungus culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010649008.6A CN111718852B (en) 2020-07-07 2020-07-07 Automatic change algae and separate fungus culture apparatus

Publications (2)

Publication Number Publication Date
CN111718852A true CN111718852A (en) 2020-09-29
CN111718852B CN111718852B (en) 2023-05-26

Family

ID=72573869

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010649008.6A Active CN111718852B (en) 2020-07-07 2020-07-07 Automatic change algae and separate fungus culture apparatus

Country Status (1)

Country Link
CN (1) CN111718852B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113403188A (en) * 2021-06-17 2021-09-17 刘正君 Anti-pollution circulation runway formula culture system based on algae is cultivated

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101050419A (en) * 2007-05-18 2007-10-10 王逢旦 Production apparatus for culturing tiny alga and production method
CN202558854U (en) * 2012-05-08 2012-11-28 青岛中仁药业有限公司 Microalgae abnormal fermenting device
CN103608103A (en) * 2011-02-07 2014-02-26 波德生物燃料公司 Light energy supply for photobioreactor system
JP2015053896A (en) * 2013-09-12 2015-03-23 本田技研工業株式会社 Reaction apparatus for biomass
CN104560695A (en) * 2015-01-21 2015-04-29 北京化工大学 Tubular photo-bioreactor based on built-in fluid driven rotor
CN106318852A (en) * 2015-06-23 2017-01-11 中国科学院上海高等研究院 Oscillating jet flow micro-bubble photobioreactor
CN206705760U (en) * 2016-10-17 2017-12-05 成都川力智能流体设备股份有限公司 A kind of pipeline Antimicrobial device
CN108018194A (en) * 2018-01-12 2018-05-11 华南理工大学 A kind of duct type bioreactor for microalgae mass culture
KR101996310B1 (en) * 2018-12-20 2019-07-04 최정환 Chlorella culture device

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101050419A (en) * 2007-05-18 2007-10-10 王逢旦 Production apparatus for culturing tiny alga and production method
CN103608103A (en) * 2011-02-07 2014-02-26 波德生物燃料公司 Light energy supply for photobioreactor system
CN202558854U (en) * 2012-05-08 2012-11-28 青岛中仁药业有限公司 Microalgae abnormal fermenting device
JP2015053896A (en) * 2013-09-12 2015-03-23 本田技研工業株式会社 Reaction apparatus for biomass
CN104560695A (en) * 2015-01-21 2015-04-29 北京化工大学 Tubular photo-bioreactor based on built-in fluid driven rotor
CN106318852A (en) * 2015-06-23 2017-01-11 中国科学院上海高等研究院 Oscillating jet flow micro-bubble photobioreactor
CN206705760U (en) * 2016-10-17 2017-12-05 成都川力智能流体设备股份有限公司 A kind of pipeline Antimicrobial device
CN108018194A (en) * 2018-01-12 2018-05-11 华南理工大学 A kind of duct type bioreactor for microalgae mass culture
KR101996310B1 (en) * 2018-12-20 2019-07-04 최정환 Chlorella culture device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李志勇等: "微藻养殖中的新型光生物反应器***", 《海湖盐与化工》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113403188A (en) * 2021-06-17 2021-09-17 刘正君 Anti-pollution circulation runway formula culture system based on algae is cultivated

Also Published As

Publication number Publication date
CN111718852B (en) 2023-05-26

Similar Documents

Publication Publication Date Title
CN102296029B (en) Perfusion bioreactor system
CN201850276U (en) Micro-filtration membrane coupling high-density culture device for lactic acid bacteria
WO2016172998A1 (en) Plant bioreactor and use method therefor
CN101353618A (en) Production method of edible fungus liquid spawn and fermentation plant thereof
TWI519640B (en) Modularized photosynthetic reactor system, algae culturing method using the same
CN111718852A (en) Automatic change alga and separate fungus culture apparatus
CN201915100U (en) Blood vessel generator of biological tissue engineering
CN114672407A (en) Anti-pollution formula bacteria culture device of clinical laboratory
CN107641594B (en) Differential pressure type open pipeline bioreactor for microalgae culture
CN111690514B (en) Automatic algae bacteria-isolation culture method
CN102127507B (en) Biological tissue engineering vasculogenesis system
CN218665971U (en) Algae bacteria-isolation circulating culture device
JP5981653B2 (en) Bioreactor vessel for large-scale growth of plants under aseptic conditions
CN103497890A (en) Perfusion culture system and method for bacteria fermentation tank
CN103548577B (en) System and method for producing and culturing liquid spawns of edible fungus
CN109251847B (en) Device and method for culturing photosynthetic microorganisms by using sunlight
CN203537926U (en) Edible fungus liquid culture production and cultivation system
KR20020012084A (en) mushroom liquid spawn fermanter
CN214032457U (en) Progressive circulating light reaction system for osmunda japonica culture
CN214694174U (en) Film type aseptic bioreactor for heterotrophic and polyculture culture of microalgae
CN213624125U (en) Bioreactor algae cultivation device
CN221094125U (en) Medical science inspection microorganism culture apparatus
CN215301804U (en) Intelligence is grown seedlings and is used seed breeding device
CN109022270B (en) Fermentation tank system for amino acid production and working method thereof
CN219449685U (en) Liquid strain fermentation tank culture solution conveying system

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant