CN103548577B - System and method for producing and culturing liquid spawns of edible fungus - Google Patents
System and method for producing and culturing liquid spawns of edible fungus Download PDFInfo
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- CN103548577B CN103548577B CN201310575209.6A CN201310575209A CN103548577B CN 103548577 B CN103548577 B CN 103548577B CN 201310575209 A CN201310575209 A CN 201310575209A CN 103548577 B CN103548577 B CN 103548577B
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Abstract
The invention discloses a system and a method for producing and culturing liquid spawns of an edible fungus. The system comprises a nutrient solution sterilized tank, a heat exchanger, a cooling-water machine set and a plurality of liquid spawn culture tanks, and further comprises a nutrient solution circulation pipeline, a nutrient solution sterilization and cooling pipeline, a nutrient solution conveying pipeline, a germ-free compressed air inlet pipeline, a blow-off pipeline and a liquid spawn conveying pipeline. The invention further discloses a method for producing and culturing the liquid spawns of the edible fungus by using the system. According to the system and the method provided by the invention, residue spawns in the liquid spawn culture tanks can be used as stock cultures for again culture, so that the infection risks due to successive vaccination can be avoided; the sterilization, the cooling, the conveying and the germ-free air supply of the spawns are completed in inclosed vessels and pipelines, so that the infection probability is zero; the movement of the liquid spawn culture tanks is avoided, so that the investment is reduced, safety and reliability are realized, the amount of used labor is reduced, and the labor intensity is reduced.
Description
Technical field
The present invention relates to making and the culture systems of bacterial classification, particularly a kind of edible fungi liquid strain makes and culture systems; The invention still further relates to and utilize this system to carry out edible fungi liquid strain making and the method for cultivating.
Background technology
In prior art, it is all adopt independently equipment that edible fungi liquid strain makes, and its making pattern is:
First strain cultivation tank is carried out manual cleaning at seeding tank cleaning shop.After cleaning terminates, enter strain cultivation tank weighing area by manually promoting strain cultivation tank.By artificial, the culture fluid prepared and clear water are poured in strain cultivation tank.Sterilizing is carried out again with manually entering seeding tank sterilizer by tank promotion strain cultivation tank.After sterilizing terminates, under the high temperature conditions strain cultivation tank is pulled out from seeding tank sterilizer inside by artificial, enter seeding tank chilling room, first filtrated air pipeline is accessed the aseptic gas interface of strain cultivation pot bottom after entering chilling room, prevent strain cultivation tank from cooling procedure, forming negative pressure, cause gas in room enter strain cultivation tank thus cause infection.Cooling is lowered the temperature by the running water method that stop-spraying is not drenched, and winter, cooling needed 8 hours.Summer, cooling needed 10-14 hour.
After cooling terminates, the filtrated air pipeline be connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, again by manually promote strain cultivation tank enter seeding tank inoculate between below hundred grades of laminar flow hood, first filtrated air pipeline in seeding tank inoculation is accessed the aseptic gas interface of 3 strain cultivation pot bottom, make to form malleation in tank, protect from infection.Afterwards under hundred grades of laminar flow hood by manually opening inoculation quick-actuating closure, mother is planted access tank in complete inoculation.
After having inoculated, first the filtrated air pipeline be connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, enter between strain cultivation by manually promoting strain cultivation tank again, after entering, by the aseptic gas interface of filtrated air pipeline access strain cultivation pot bottom between strain cultivation, mycelia oxygen supply in tank.Cultivate one-period after 7-8 days, bacterial classification output channel is connected with body Spawn incubation pot bottom bacterial classification output interface, cultured liquid spawn is flowed to inoculating facility.After inoculation terminates.The strain cultivation tank of sky is manually pushed to seeding tank cleaning shop clean, recycles.
Mainly there is following defect in this liquid spawn making apparatus of the prior art and operator scheme:
1, working strength is large: because each bacteria culture tank need install castor, manually pull carrying, so gross weight can only below one ton, or manpower is difficult to drive;
2, infection rate is high: each seeding tank needs to patch for 3 times in a cultivation cycle, dismantle filtrated air joint, patch bacterial classification output channel joint for 1 time, successively open the inoculation of inoculation quick-actuating closure.Above process all must aseptically complete, and requires that the technical ability of employee is very skilled, otherwise very easily causes infection;
3, cost of investment is high.Within 8 days, calculate by 3 tanks, cultivation cycle by every day.Separately add 3 non-conservation tanks, amount to 3 × 8+3=27 tank.Sterilizing need be equipped with sterilizer special;
4, water consumption is large, and during cooling, running water not stop-spraying pouring seeding tank, trickles in vain, can not recycle, cause waste.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, provides a kind of reasonable in design, safe, the edible fungi liquid strain that greatly reduces infection rate easy to use makes and culture systems.
Another technical problem to be solved by this invention is to provide application said system and carries out edible fungi liquid strain making and the method for cultivating.
Technical problem to be solved by this invention is realized by following technical scheme.The present invention is that a kind of edible fungi liquid strain makes and culture systems, is characterized in: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline accesses heat exchanger and picks out from carrying hot device, this road is connected with into steam pipework;
Nutrient solution sterilization tank or nutrient solution circulation line are connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units
,the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline accesses heat exchanger and picks out from carrying hot device, this road is connected with into steam pipework and hydrophobic exhaust line;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, and import is connected with the entrance of each strain cultivation tank;
Enter sterile compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and is discharged by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and is exported by liquid spawn delivery outlet.
In system of the present invention, preferred technical scheme is further:
1. the heat exchanger described in is heat-exchangers of the plate type;
2. on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor;
3. be provided with bottom nutrient solution sterilization tank and be provided with bilge discharge pipe road;
4. be connected with on liquid spawn transfer pipeline into steam pipework and inlet pipeline;
5. on nutrition pipeline road, be connected with inlet pipeline;
6. entering on sterile compressed gas pipeline to be connected with pressure relief pipe;
7. in strain cultivation tank, bottom is provided with agitator;
8. the agitator described in is preferably agitator air, and described agitator air comprises and stirs back shaft and establish puddler thereon, puddler be each side provided with the contrary pore group at least one group of perforate direction.
In present system, each pipeline arranges traditional valve on demand or instrument controls.According to arranging conveniently between each pipeline, the mode of shared one section of pipeline can be adopted to settle.
Technical problem to be solved by this invention is realized by following technical scheme.The invention also discloses a kind of any one that use in above technical scheme described in system carry out bacterial classification make with cultivate method, be characterized in, its step is as follows:
(1) sterile nutrient solution makes:
A. add water: prepare nutrient solution in proportion, and it is added in tank body by the charge door of nutrient solution sterilization tank, start on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, add running water quantitative adjustment concentration;
B. heat up: start nutrient solution circulation line, circulate in nutrition liquid pump heat exchanger; Nutrient solution sterilizing simultaneously and cooling line, the nutrient solution in heat exchanger heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount entering steam during intensification sterilizing controls by temperature, opens lower than during setting value, closes higher than during setting value;
D. cool: after sterilizing terminates, steam off pipeline, to be pumped in heat exchanger by the cold water of handpiece Water Chilling Units by nutrient solution sterilizing and cooling line and circulate, nutrient solution also carries out circulating cooling in heat exchanger simultaneously, when solution temperature is reduced to setting value, cooling completes;
E. sterile nutrient solution conveying: open nutrition pipeline road and enter sterile compressed gas pipeline, nutrient solution being delivered to respectively in strain cultivation tank;
F. nutrient solution sterilization tank cleaning: open on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, injected clear water in tank, carry out wash cycles, heat exchanger and nutrient solution sterilization tank are cleaned;
(2) cultural hypha: after nutrient solution enters strain cultivation tank, aseptically the inoculation block of strain cultivation tank is uncapped and is planted in access tank by mother; Open into sterile compressed gas pipeline during cultivation, aseptic gas is sent in strain cultivation tank, to mycelia oxygen supply;
When being equipped with agitator air, the puddler of the agitator air of liquid spawn transfer pipeline is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranged can make puddler rotate, and realizes stirring;
(3) liquid spawn exports: after cultural hypha completes, open strain cultivation tank, exported by liquid spawn by liquid spawn transfer pipeline; After exporting, in tank, remaining part bacterial classification uses when female kind, need not connect new mother's kind again when injecting new nutrient solution;
(4) strain cultivation tank cleaning and sterilizing: the mother in strain cultivation tank plants the degeneration considering spawn activity after lasting use a period of time, mother's kind that need more renew; Before renewing female kind, need carry out cleaning and sterilizing to strain cultivation tank, its method is as follows:
A. clean: open liquid spawn transfer pipeline, by the emptying of residual liquid bacterial classification; By nutrition pipeline road, running water is sent into the strain cultivation tank of required cleaning, aseptic gas is sent in tank simultaneously and clean, after cleaning, by blowoff line, sewage is discharged;
B. sterilize: after cleaning, by nutrition pipeline road, high-temperature steam is sent in the strain cultivation tank of required sterilizing, carry out sterilizing;
(5) nutrition pipeline road, liquid spawn transfer pipeline cleaning and sterilizing: after each conveying terminates, cleaning and sterilizing need be carried out to nutrition pipeline road, liquid spawn transfer pipeline;
A. nutrition pipeline road cleaning and sterilizing: running water is sent into nutrition pipeline road and clean; After cleaning terminates, high-temperature steam is sent into nutrition pipeline road and carry out disinfection;
B. liquid spawn transfer pipeline cleaning and sterilizing: running water is sent into liquid spawn transfer pipeline and clean; After cleaning terminates, high-temperature steam is sent into liquid spawn transfer pipeline and carry out disinfection.
Compared with prior art, the advantage of technical solution of the present invention is mainly reflected in:
1. present system can the making of complete independently bacterial classification and cultivation, is a kind of production of hybrid seeds equipment of integrated form.
2. the supply of the sterilizing of bacteria culture fluid, cooling, conveying and filtrated air all completes in airtight container and pipeline, and infecting probability is zero.
3. strain cultivation tank is without the need to moving, and can customize the specification of tank body according to user's consumption every day.
4. investment can be reduced: do not need liquid spawn tank sterilizer.The more former project plan comparison of strain cultivation tank quantity has large batch of minimizing, but the volume of each strain cultivation tank will meet this factory's consumption of a day.
5. liquid spawn tank does not need cleaning in principle, general annual cleaning 1-2 time.
6. the remaining bacterial classification in strain cultivation tank can be planted use as the mother again cultivated, avoid the infection risk successively inoculated, greatly reduce and infect probability.
7. present system can be controlled automatically by computer, safe and reliable, reduces the amount of labour used, reduces labour intensity.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of present system;
Fig. 2 is a kind of plan structure schematic diagram of agitator.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1, with reference to Fig. 1 and 2, a kind of edible fungi liquid strain makes and culture systems, and this system comprises nutrient solution sterilization tank 1, heat exchanger 10, handpiece Water Chilling Units 3 and some strain cultivation tanks 7; And,
Nutrient solution circulation line 4: its one end is connected to the top of nutrient solution sterilization tank 1, the other end is connected to the bottom of nutrient solution sterilization tank 1; This pipeline is provided with circulating pump, and this pipeline accesses heat exchanger 10 and picks out from carrying hot device, this road is connected with into steam pipework;
Nutrient solution sterilization tank 1 or nutrient solution circulation line 4 are connected with inlet pipeline;
Nutrient solution sterilizing and cooling line 2: its one end is connected with the water inlet of handpiece Water Chilling Units 3
,the other end is connected with the delivery port of handpiece Water Chilling Units 3; This pipeline is provided with coolant pump, and this pipeline accesses heat exchanger 10 and picks out from carrying hot device, this road is connected with into steam pipework and hydrophobic exhaust line;
Nutrition pipeline road 8: its outlet is connected to the bottom of nutrient solution sterilization tank 1, and import is connected with the entrance of each strain cultivation tank 7;
Enter sterile compressed gas pipeline 5: it is connected with the top of nutrient solution sterilization tank 1, the bottom of each strain cultivation tank 7 respectively;
Blowoff line 9: its entrance point is connected with the bottom of each strain cultivation tank 7 respectively, and is discharged by sewage draining exit;
Liquid spawn transfer pipeline 6: its entrance point is connected respectively with bottom each strain cultivation tank 7, and is exported by liquid spawn delivery outlet.
Embodiment 2, in the system described in embodiment 1: described heat exchanger 10 is heat-exchangers of the plate type.
Embodiment 3, in the system described in embodiment 1 or 2: be provided with liquid level gauge and temperature sensor on nutrient solution sterilization tank 1.
Embodiment 4, in the system of embodiment 1-3 described in any one: be provided with bottom nutrient solution sterilization tank 1 and be provided with bilge discharge pipe road.
Embodiment 5, in the system of embodiment 1-4 described in any one: be connected with into steam pipework and inlet pipeline on liquid spawn transfer pipeline 6.
Embodiment 6, in the system of embodiment 1-5 described in any one: be connected with inlet pipeline on nutrition pipeline road 8.
Embodiment 7, in the system of embodiment 1-6 described in any one: entering on sterile compressed gas pipeline 5 to be connected with pressure relief pipe.
Embodiment 8, in the system of embodiment 1-7 described in any one: in strain cultivation tank 7, bottom is provided with agitator 14.
Embodiment 9, in the system of embodiment 1-8 described in any one: described agitator 14 is agitator air, described agitator air comprises the puddler 13 stirring back shaft 11 and establish thereon, puddler 13 be each side provided with the contrary pore group 12 at least one group of perforate direction.
Embodiment 10, a kind ofly uses the system of embodiment 1-9 described in any one to carry out bacterial classification to make and the method for cultivating, and its step is as follows:
(1) sterile nutrient solution makes:
A. add water: prepare nutrient solution in proportion, and it is added in tank body by the charge door of nutrient solution sterilization tank 1, start on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, add running water quantitative adjustment concentration;
B. heat up: start nutrient solution circulation line 4, circulate in nutrition liquid pump heat exchanger 10; Nutrient solution sterilizing simultaneously and cooling line 2, the nutrient solution in heat exchanger 10 heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount entering steam during intensification sterilizing controls by temperature, opens lower than during setting value, closes higher than during setting value;
D. cool: after sterilizing terminates, steam off pipeline, to be pumped in heat exchanger 10 by the cold water of handpiece Water Chilling Units 3 by nutrient solution sterilizing and cooling line 2 and circulate, nutrient solution also carries out circulating cooling in heat exchanger 10 simultaneously, when solution temperature is reduced to setting value, cooling completes;
E. sterile nutrient solution conveying: open nutrition pipeline road 8 and enter sterile compressed gas pipeline 5, nutrient solution being delivered to respectively in strain cultivation tank 7;
F. nutrient solution sterilization tank 1 cleans: open on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, injected clear water in tank, carry out wash cycles, heat exchanger 10 and nutrient solution sterilization tank 1 are cleaned;
(2) cultural hypha: after nutrient solution enters strain cultivation tank 7, aseptically the inoculation block of strain cultivation tank 7 is uncapped and is planted in access tank by mother; Open into sterile compressed gas pipeline 5 during cultivation, aseptic gas is sent in strain cultivation tank 7, to mycelia oxygen supply;
When being equipped with agitator air, the puddler of the agitator air of liquid spawn transfer pipeline 6 is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranged can make puddler rotate, and realizes stirring;
(3) liquid spawn exports: after cultural hypha completes, open strain cultivation tank 7, exported by liquid spawn by liquid spawn transfer pipeline 6; After exporting, in tank, remaining part bacterial classification uses when female kind, need not connect new mother's kind again when injecting new nutrient solution;
(4) strain cultivation tank 7 cleaning and sterilizing: the mother in strain cultivation tank 7 plants the degeneration considering spawn activity after lasting use a period of time, mother's kind that need more renew; Before renewing female kind, need carry out cleaning and sterilizing to strain cultivation tank 7, its method is as follows:
A. clean: open liquid spawn transfer pipeline 6, by the emptying of residual liquid bacterial classification; By nutrition pipeline road 8, running water is sent into the strain cultivation tank 7 of required cleaning, aseptic gas is sent in tank simultaneously and clean, after cleaning, by blowoff line 9, sewage is discharged;
B. sterilize: after cleaning, by nutrition pipeline road 8, high-temperature steam is sent in the strain cultivation tank 7 of required sterilizing, carry out sterilizing;
(5) nutrition pipeline road 8, liquid spawn transfer pipeline 6 cleaning and sterilizing: after each conveying terminates, cleaning and sterilizing need be carried out to nutrition pipeline road 8, liquid spawn transfer pipeline 6;
A. nutrition pipeline road 8 cleaning and sterilizing: running water is sent into nutrition pipeline road 8 and clean; After cleaning terminates, high-temperature steam is sent into nutrition pipeline road 8 and carry out disinfection;
B. liquid spawn transfer pipeline 6 cleaning and sterilizing: running water is sent into liquid spawn transfer pipeline 6 and clean; After cleaning terminates, high-temperature steam is sent into liquid spawn transfer pipeline 6 and carry out disinfection.
Claims (9)
1. edible fungi liquid strain makes and a culture systems, it is characterized in that: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline accesses heat exchanger and picks out from heat exchanger, this road is connected with into steam pipework; Nutrient solution sterilization tank or nutrient solution circulation line are connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units, the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline accesses heat exchanger and picks out from heat exchanger, this road is connected with into steam pipework and hydrophobic exhaust line;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, and import is connected with the entrance of each strain cultivation tank;
Enter sterile compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and is discharged by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and is exported by liquid spawn delivery outlet;
Using this system to carry out bacterial classification makes with the method step cultivated as follows:
(1) sterile nutrient solution makes:
A. add water: prepare nutrient solution in proportion, and it is added in tank body by the charge door of nutrient solution sterilization tank, start the inlet pipeline that nutrient solution sterilization tank or nutrient solution circulation line connect, add running water quantitative adjustment concentration;
B. heat up: start nutrient solution circulation line, circulate in nutrition liquid pump heat exchanger; Nutrient solution sterilizing simultaneously and cooling line, the nutrient solution in heat exchanger heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount entering steam during intensification sterilizing controls by temperature, opens lower than during setting value, closes higher than during setting value;
D. cool: after sterilizing terminates, steam off pipeline, to be pumped in heat exchanger by the cold water of handpiece Water Chilling Units by nutrient solution sterilizing and cooling line and circulate, nutrient solution also carries out circulating cooling in heat exchanger simultaneously, when solution temperature is reduced to setting value, cooling completes;
E. sterile nutrient solution conveying: open nutrition pipeline road and enter sterile compressed gas pipeline, nutrient solution being delivered to respectively in strain cultivation tank;
F. nutrient solution sterilization tank cleaning: open the inlet pipeline that nutrient solution sterilization tank or nutrient solution circulation line connect, injected clear water in nutrient solution sterilization tank, carries out wash cycles, heat exchanger and nutrient solution sterilization tank is cleaned;
(2) cultural hypha: after nutrient solution enters strain cultivation tank, aseptically mother plants in access tank by the inoculation quick-actuating closure of strain cultivation tank; Open into sterile compressed gas pipeline during cultivation, aseptic gas is sent in strain cultivation tank, to mycelia oxygen supply;
When being equipped with agitator air, the puddler of the agitator air of liquid spawn transfer pipeline is communicated with, and is evenly fed in tank by aseptic gas, and the pore group oppositely arranged can make puddler rotate, and realizes stirring;
(3) liquid spawn exports: after cultural hypha completes, open strain cultivation tank, exported by liquid spawn by liquid spawn transfer pipeline; After exporting, in tank, remaining part bacterial classification uses when female kind, need not connect new mother's kind again when injecting new nutrient solution;
(4) strain cultivation tank cleaning and sterilizing: the mother in strain cultivation tank plants the degeneration considering spawn activity after lasting use a period of time, mother's kind that need more renew; Before renewing female kind, need carry out cleaning and sterilizing to strain cultivation tank, its method is as follows:
A. clean: open liquid spawn transfer pipeline, by the emptying of residual liquid bacterial classification; By nutrition pipeline road, running water is sent into the strain cultivation tank of required cleaning, aseptic gas is sent in tank simultaneously and clean, after cleaning, by blowoff line, sewage is discharged;
B. sterilize: after cleaning, by nutrition pipeline road, high-temperature steam is sent in the strain cultivation tank of required sterilizing, carry out sterilizing;
(5) nutrition pipeline road, liquid spawn transfer pipeline cleaning and sterilizing: after each conveying terminates, cleaning and sterilizing need be carried out to nutrition pipeline road, liquid spawn transfer pipeline;
A. nutrition pipeline road cleaning and sterilizing: running water is sent into nutrition pipeline road and clean; After cleaning terminates, high-temperature steam is sent into nutrition pipeline road and carry out disinfection;
B. liquid spawn transfer pipeline cleaning and sterilizing: running water is sent into liquid spawn transfer pipeline and clean; After cleaning terminates, high-temperature steam is sent into liquid spawn transfer pipeline and carry out disinfection.
2. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: described heat exchanger is heat-exchangers of the plate type.
3. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor.
4. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: bottom nutrient solution sterilization tank, be provided with bilge discharge pipe road.
5. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: be connected with into steam pipework and inlet pipeline on liquid spawn transfer pipeline.
6. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: on nutrition pipeline road, be connected with inlet pipeline.
7. edible fungi liquid strain according to claim 1 makes and culture systems, it is characterized in that: entering on sterile compressed gas pipeline to be connected with pressure relief pipe.
8. make and culture systems according to the edible fungi liquid strain of claim 1-7 described in any one, it is characterized in that: bottom is provided with agitator in strain cultivation tank.
9. edible fungi liquid strain according to claim 8 makes and culture systems, it is characterized in that: described agitator is agitator air, described agitator air comprises and stirs back shaft and establish puddler thereon, puddler be each side provided with the contrary pore group at least one group of perforate direction.
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| CN107318626A (en) * | 2017-08-01 | 2017-11-07 | 深圳前海弘稼科技有限公司 | Nutrient solution cooling system |
| CN107493969A (en) * | 2017-09-01 | 2017-12-22 | 苏州顺泰元虫草生物科技有限公司 | A kind of high-efficient culture method of cordyceps sinensis |
| CN108076726B (en) * | 2017-12-28 | 2023-08-25 | 江苏哈工药机科技股份有限公司 | Sterile culture system for ginseng seeds |
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|---|---|---|---|---|
| CN2720798Y (en) * | 2004-08-31 | 2005-08-31 | 锁现民 | Combined continuous fermenting producer for liquid culture of edible mushroom |
| CN2754377Y (en) * | 2004-05-31 | 2006-02-01 | 吴甲申 | Household high-efficient continuous integrated producing apparatus for edible mushroom liquid bacteria |
| CN202322818U (en) * | 2011-11-11 | 2012-07-11 | 黑龙江东方学院 | Microorganism stirring device |
| CN203537926U (en) * | 2013-11-18 | 2014-04-16 | 连云港国鑫食用菌成套设备有限公司 | Edible fungus liquid culture production and cultivation system |
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| JP5341540B2 (en) * | 2009-02-02 | 2013-11-13 | 敏昭 春山 | Mushroom liquid inoculum culture apparatus and method for culturing mushroom liquid inoculum |
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| CN2754377Y (en) * | 2004-05-31 | 2006-02-01 | 吴甲申 | Household high-efficient continuous integrated producing apparatus for edible mushroom liquid bacteria |
| CN2720798Y (en) * | 2004-08-31 | 2005-08-31 | 锁现民 | Combined continuous fermenting producer for liquid culture of edible mushroom |
| CN202322818U (en) * | 2011-11-11 | 2012-07-11 | 黑龙江东方学院 | Microorganism stirring device |
| CN203537926U (en) * | 2013-11-18 | 2014-04-16 | 连云港国鑫食用菌成套设备有限公司 | Edible fungus liquid culture production and cultivation system |
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