CN111670896A

CN111670896A - Neutral buffer tissue fixing solution

Info

Publication number: CN111670896A
Application number: CN202010347214.1A
Authority: CN
Inventors: 王洪柱; 江川; 陈建华; 徐雪芬; 暴忠坤
Original assignee: Womens Hospital of Zhejiang University School of Medicine
Current assignee: Womens Hospital of Zhejiang University School of Medicine
Priority date: 2020-04-28
Filing date: 2020-04-28
Publication date: 2020-09-18

Abstract

The invention discloses a neutral buffer tissue fixing solution which comprises the following components in parts by weight: 25-35 parts of formaldehyde; 8-15 parts of ethanol; 5-10 parts of 2-methyl-1, 2-phenylisothiazole-3-ketone; 5-15 parts of tert-butyl alcohol; 0.05-0.2 part of glacial acetic acid; 5-15 parts of phosphate; 3-8 parts of sodium chloride; 250-350 parts of deionized water; the pH value is 6.5-7.4. The formaldehyde volatilization degree is obviously reduced, the industry gold standard of 10% of formaldehyde is kept, the formaldehyde volatilization is inhibited to a certain degree, and the damage degree of workers is reduced.

Description

Neutral buffer tissue fixing solution

Technical Field

The invention relates to the technical field of biology, in particular to neutral buffer tissue fixing solution.

Background

The formalin tissue fixing solution is widely applied in the fields of medical treatment and biological engineering, and is used for fixing and preserving biological tissue specimens. In the prior art, tissue specimens are soaked and fixed in neutral formalin. The main component is formaldehyde, which can be combined with amino group in tissue protein to denature protein and harden tissue. One of the formulations is 40% formaldehyde 100ml, sodium dihydrogen phosphate (or anhydrous potassium dihydrogen phosphate) 4.0g, disodium hydrogen phosphate 6.5g, and distilled water 900 ml. Because the neutral formalin is added with the phosphate buffer solution, the neutral formalin is not easy to be oxidized, and the pH value can be kept to be about 7.0 for a long time, the neutral formalin has very favorable effect on fixing (particularly fixing for a long time) staining the tissue specimen.

However, formaldehyde has an irritating odor and can be smelled at low concentrations, and its main harm is manifested as irritation to the skin mucosa. When the formaldehyde reaches a certain concentration indoors, people feel uncomfortable. Greater than 0.08mg/m³The formaldehyde concentration can cause redness, itching, discomfort or pain in the throat, hoarseness, sneezing, chest distress, asthma, dermatitis, etc. Long-term and low-concentration formaldehyde contact can cause headache, dizziness, hypodynamia, sensory disturbance and immunity reduction, and can cause sleepiness, hypomnesis or neurasthenia and mental depression. The harm of chronic poisoning to the respiratory system is also huge, and the long-term exposure to formaldehyde can cause respiratory dysfunction and hepatotoxic lesion, which are manifested as hepatocyte injury, liver radiation energy abnormality and the like. Although the content of formaldehyde in the currently general tissue fixative is 3.7-4.0%, formaldehyde is volatile in the using process, so that the effective time of the tissue fixative is short. In the actual use process, the content of formaldehyde in the environment far exceeds the range allowed by national standards, and the long-term contact causes serious harm to the health of medical staff.

Therefore, how to reduce the volatilization of formaldehyde during the process of using the tissue fixative, maintain the characteristic of fixing the tissue specimen, reduce the harm to medical staff and provide the tissue fixative for removing or reducing the irritant odor of formaldehyde is a new subject to be researched currently_。

Disclosure of Invention

The invention aims to solve the technical problems and provides neutral buffer tissue fixing liquid.

The invention is realized according to the following technical scheme.

A neutral buffered tissue fixative comprising the following components: formaldehyde, ethanol, 2-methyl-1, 2-phenylisothiazole-3-ketone, tert-butyl alcohol, glacial acetic acid, phosphate, sodium chloride and deionized water.

Further, the neutral buffer tissue fixing solution comprises the following components in parts by weight:

the pH value is 6.5-7.4.

Preferably, the neutral buffer tissue fixing solution comprises the following components in parts by weight:

the pH value is 6.5-7.4.

The invention has the following beneficial effects:

the formaldehyde volatilization degree is obviously reduced, the industrial gold standard of 10% of formaldehyde is kept, the formaldehyde volatilization is inhibited to a certain degree, the damage degree of workers is reduced, and the formaldehyde volatilization inhibitor has a wide application prospect.

Detailed Description

In order to further explain the technical solutions of the present invention and the technical effects achieved by the technical solutions, the present invention will be further described with reference to the following embodiments.

the pH value is 6.5-7.4.

the pH value is 6.5-7.4.

Example 1

A neutral buffer tissue fixing solution comprises the following components in parts by weight:

the pH was 7.2.

Example 2

the pH was 6.8.

Example 3

the pH was 7.0.

Example 4

the pH was 6.9.

Example 5

The tissue samples prepared in examples 1 to 3 were subjected to sectioning and staining treatment, and the treatment effect was observed, and the test results are shown in table 1.

TABLE 1

	Quality of slice	Under mirror effect
			Example 1	The slice is relatively flat and not easy to be broken	The tissue structure under the mirror is more complete
Example 2	The slice is relatively flat and not easy to be broken	The tissue structure under the mirror is more complete
			Example 3	The slice is very smooth and is not easy to break	The tissue structure under the mirror is very complete and clear
Example 4	The slice is smooth and not easy to break	The tissue structure under the mirror is complete and clear

The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.

Claims

1. A neutral buffer tissue fixative, comprising: comprises the following components: formaldehyde, ethanol, 2-methyl-1, 2-phenylisothiazole-3-ketone, tert-butyl alcohol, glacial acetic acid, phosphate, sodium chloride and deionized water.

2. The neutral buffered tissue fixative of claim 1, wherein: comprises the following components in parts by weight:

25-35 parts of formaldehyde;

8-15 parts of ethanol;

5-10 parts of 2-methyl-1, 2-phenylisothiazole-3-ketone;

5-15 parts of tert-butyl alcohol;

0.05-0.2 part of glacial acetic acid;

5-15 parts of phosphate;

3-8 parts of sodium chloride;

250-350 parts of deionized water;

the pH value is 6.5-7.4.

3. The neutral buffered tissue fixative of claim 1, wherein: comprises the following components in parts by weight:

30 parts of formaldehyde;

12 parts of ethanol;

8 parts of 2-methyl-1, 2-phenylisothiazole-3-ketone;

10 parts of tert-butyl alcohol;

glacial acetic acid 0.1 part;

10 parts of phosphate;

6 parts of sodium chloride;

300 parts of deionized water;

the pH value is 6.5-7.4.