CN111587968A - Functional feed for improving growth and immunity of micropterus salmoides - Google Patents
Functional feed for improving growth and immunity of micropterus salmoides Download PDFInfo
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Abstract
The invention relates to the field of aquatic feeds, in particular to a functional feed for improving growth and immunity of micropterus salmoides. A functional feed for improving growth and immunity of Micropterus salmoides comprises fish meal, flour, soybean meal, wheat gluten, cerevisiae Fermentum, fish oil, soybean lecithin, calcium dihydrogen phosphate, vitamins, minerals, choline chloride, and immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris. The compound immunopotentiator can improve the physique of the micropterus salmoides and enhance the resistance of the micropterus salmoides to pathogenic microorganisms. The feed additive can not only obviously enhance the immunity resistance of the fish, but also improve the nutritional value of the cultured fish when being added into the feed. Can reduce or eliminate the use of antibiotics and reduce the pollution to the environment.
Description
Technical Field
The invention relates to the field of aquatic feeds, in particular to a functional feed for improving growth and immunity of micropterus salmoides.
Background
Micropterus salmoides (Micropterus salmoides) are freshwater carnivorous fishes which are widely cultured worldwide and are one of the fishes with the fastest culture yield in China. At present, research on the formula of micropterus salmoides feed mostly focuses on the requirements of the micropterus salmoides feed on three nutrient substances (protein, fat and carbohydrate), and when single high-nutrient feed is fed for a long time in the whole culture period, health problems of liver dysfunction, anorexia, enteritis and the like of micropterus salmoides in different degrees are caused, finally, the disease resistance and the growth performance are reduced, and even a great amount of death of the micropterus salmoides is caused in severe cases. The main targets of aquaculture development are fast growth rate and high yield, and intensive high-density culture mode is widely applied to culture of micropterus salmoides in pursuit of high yield. As the water environment is destroyed by wide industrial development and the water quality is continuously deteriorated, the micropterus salmoides are more easily attacked by bacterial diseases and virus diseases in an intensive high-density culture mode, and farmers are disastrous. At present, more than 80% of micropterus salmoides in China are mainly cultured by ice fresh wild trash fish, even if the ice fresh wild trash fish is used, diseases are frequent in the culture of micropterus salmoides, and some antibiotics are used in the culture link. The use of antibiotics can cause the residues in aquatic products, can cause the occurrence of drug resistance of pathogenic bacteria of human bodies, and reduces the resistance of the human bodies to the pathogenic bacteria. Antibiotic drugs such as chloramphenicol, erythromycin, bacitracin zinc, tylosin, avomycin, vancomycin and the like have been regarded as forbidden fishery drugs by the Ministry of agriculture, and are strictly forbidden to be used in all links such as aquaculture, processing and circulation. Therefore, it is important to develop a highly nutritious feed capable of enhancing immunity to protect liver and intestinal health of fish.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the functional feed for improving the growth and immunity of the micropterus salmoides.
The purpose of the invention is realized by the following technical scheme:
a functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 40-50 parts of fish meal, 15-25 parts of flour, 15-20 parts of bean pulp, 1-5 parts of wheat gluten, 1-5 parts of beer yeast, 3-6 parts of fish oil, 0.5-2 parts of soybean lecithin, 0.5-2 parts of calcium dihydrogen phosphate, 0.5-2 parts of vitamin, 0.5-2 parts of mineral matter, 0.2-1 part of choline chloride and 2-10 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, wherein the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:3-1: 7.
Preferably, the feed comprises the following components in parts by weight: 45 parts of fish meal, 20 parts of flour, 16.3 parts of soybean meal, 3 parts of vital gluten, 3 parts of beer yeast, 5 parts of fish oil, 1 part of soybean lecithin, 1 part of monocalcium phosphate, 1.2 parts of vitamin, 1 part of mineral substance, 0.5 part of choline chloride and 3 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, wherein the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1: 5.
Preferably, the vitamins comprise the following components in parts by weight: VB19-12 parts of VB230-35 parts of VB610-25 parts of VB120.05-0.3 part and VK35-12 parts of inositol 500-31-7 parts and 500 parts of VC 100-.
Preferably, the vitamins comprise the following components in parts by weight: VB19.6 parts and VB232 parts and VB620 parts and VB120.1 part of VK310 parts of inositol 800 parts, pantothenic acid 60 parts, nicotinic acid 200 parts, folic acid 20 parts, VH1.2 parts and VD35 parts and VC200 parts.
Preferably, the mineral comprises the following components in parts by weight: 1-3 parts of NaF, 0.3-1 part of KI and CoCl2·6H230-70 parts of O and CuSO4·5H25-15 parts of O and FeSO4·H270-100 parts of O, ZnSO4·H220-60 parts of O and MnSO4·H250-80 parts of O and MgSO4·7H21500 portions of O1000-2PO4)2·H24000 parts of O1000-.
Preferably, the mineral comprises the following components in parts by weight: NaF 2 parts, KI 0.8 parts and CoCl2·6H2O50 parts and CuSO4·5H2O10 parts, FeSO4·H2O80 parts, ZnSO4·H250 portions of O and MnSO4·H2O60 parts, MgSO4·7H2O1200 parts, Ca (H)2PO4)2·H23000 parts of O and 100 parts of NaCl.
Preferably, the haematococcus pluvialis contains 2 to 4% by mass of astaxanthin.
Preferably, the content of adenosine in the cordyceps militaris is 500-1000ug/g, and the content of cordyceps polysaccharide is 20-60 mg/g.
Haematococcus pluvialis and Cordyceps militaris can be cultured by the following method:
the method for culturing Haematococcus pluvialis GXU11 comprises the following steps: inoculating the algae seeds to
Introducing 1-2% CO into the glass column-shaped photobioreactor at a culture temperature of 20-30 deg.C2The air of (2) was cultured with stirring in BBM medium, CO (NH)2)2Is a nitrogen source, the initial nitrogen concentration is 18mmol/L, the illumination intensity is 100--2·s-1Culturing for 10-15 days, standing to remove supernatant, adding fresh BBM medium without nitrogen source, 300--2·s-1Culturing for 10-15 days, centrifuging and drying to obtain algae powder, wherein the astaxanthin content in the cultured haematococcus pluvialis algae powder is 2-4%.
The method for culturing the hypha of the Cordyceps militaris (cordyces militaris GXUF2) comprises the following steps: the strain is inoculated in a 1000mL triangular flask, the culture medium is 30-50% of rice, 20-30% of bran, 10-20% of peptone, 10-20% of corn flour and 5-10% of yeast powder, 200 mL of water is added, and the strain is cultured for 30-50 days at the temperature of 25-30 ℃ until hyphae grow over the whole flask. Collecting mycelium and drying to obtain cordyceps militaris powder, wherein the adenosine content of the powder is 500-1000ug/g, and the cordyceps polysaccharide content is 20-60 mg/g.
The feed is added with a compound immunopotentiator of haematococcus pluvialis and cordyceps militaris. Haematococcus pluvialis is rich in astaxanthin, linoleic acid and linolenic acid as well as other nutrients and active components. The astaxanthin has multiple biological effects of resisting oxidation, inflammation, cancer, and immunity. The cordyceps militaris and the expensive cordyceps sinensis belong to the cordyceps genus, but the cordyceps militaris and the expensive cordyceps sinensis can be massively fermented and cultured, and the price of the cordyceps militaris is far lower than that of the cordyceps sinensis. Cordyceps militaris has multiple effects of enhancing immunity, resisting bacteria, resisting cancer, resisting anoxia, resisting aging and the like. The compound immunopotentiator can improve the physique of the micropterus salmoides and enhance the resistance of the micropterus salmoides to pathogenic microorganisms. The feed additive can not only obviously enhance the immunity resistance of the fish, but also improve the nutritional value of the cultured fish when being added into the feed. Can reduce or eliminate the use of antibiotics and reduce the pollution to the environment.
Drawings
FIG. 1 is a graph of the effect of a complex immunopotentiator on the morphology of the liver of Micropterus salmoides;
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the embodiments of the present invention are not limited to the scope of the examples. These examples are intended to illustrate the invention only and are not intended to limit the scope of the invention. In addition, various modifications may occur to those skilled in the art upon reading the present disclosure, and such equivalent variations are within the scope of the present invention as defined in the appended claims.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
A functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 40 parts of fish meal, 15 parts of flour, 15 parts of soybean meal, 1 part of vital gluten, 1 part of beer yeast, 3 parts of fish oil, 0.5 part of soybean lecithin, 0.5 part of monocalcium phosphate, 0.5 part of vitamin, 0.5 part of mineral substance, 0.2 part of choline chloride and 2 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:3, the mass content of astaxanthin contained in the haematococcus pluvialis is 2.33%, the content of adenosine contained in the cordyceps militaris is 565ug/g, and the content of cordyceps polysaccharide is 23.8 mg/g.
The vitamins comprise the following components in parts by weight: VB19 parts and VB230 portions and VB610 parts and VB120.05 part and VK35 parts of inositol 500 parts, pantothenic acid 20 parts, nicotinic acid 100 parts, folic acid 10 parts, VH 0.8 parts and VD31 part and VC 100 parts.
The mineral comprises the following components in parts by weight: 1 part of NaF, 0.3 part of KI and CoCl2·6H230 portions of O and CuSO4·5H2O5 parts, FeSO4·H270 parts of O, ZnSO4·H2O20 parts and MnSO4·H2O50 parts, MgSO4·7H2O1000 parts, Ca (H)2PO4)2·H21000 parts of O and 50 parts of NaCl.
The prepared feed has the following nutritional levels: 11.16% of crude fat, 41.89% of crude protein and 13.56% of carbohydrate.
Example 2
A functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 45 parts of fish meal, 20 parts of flour, 16.3 parts of soybean meal, 3 parts of vital gluten, 3 parts of beer yeast, 5 parts of fish oil, 1 part of soybean lecithin, 1 part of monocalcium phosphate, 1.2 parts of vitamin, 1 part of mineral substance, 0.5 part of choline chloride and 3 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:5, the mass content of astaxanthin contained in the haematococcus pluvialis is 3%, the content of adenosine contained in the cordyceps militaris is 689ug/g, and the content of cordyceps polysaccharide is 40.4 mg/g.
The vitamins comprise the following components in parts by weight: VB19.6 parts and VB232 parts and VB620 parts and VB120.1 part of VK310 parts of inositol 800 parts, pantothenic acid 60 parts, nicotinic acid 200 parts, folic acid 20 parts, VH1.2 parts and VD35 parts and VC200 parts.
The mineral comprises the following components in parts by weight: NaF 2 parts, KI 0.8 parts and CoCl2·6H 250 portions of O and CuSO4·5H2O10 parts, FeSO4·H2O80 parts, ZnSO4·H 250 portions of O and MnSO4·H2O60 parts, MgSO4·7H2O1200 parts, Ca (H)2PO4)2·H23000 parts of O and 100 parts of NaCl.
The prepared feed has the following nutritional levels: 12.38% of crude fat, 42.26% of crude protein and 12.49% of carbohydrate.
Example 3
A functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 50 parts of fish meal, 25 parts of flour, 20 parts of soybean meal, 5 parts of vital gluten, 5 parts of beer yeast, 6 parts of fish oil, 2 parts of soybean lecithin, 2 parts of monocalcium phosphate, 2 parts of vitamin, 2 parts of mineral substances, 1 part of choline chloride and 10 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:7, the mass content of astaxanthin contained in the haematococcus pluvialis is 4%, the content of adenosine contained in the cordyceps militaris is 715ug/g, and the content of cordyceps polysaccharide is 51.7 mg/g.
The vitamins comprise the following components in parts by weight: VB112 parts and VB235 parts and VB625 parts and VB120.3 part of VK312 parts of inositol, 1000 parts of pantothenic acid, 70 parts of nicotinic acid, 300 parts of folic acid, 30 parts of VH 1.5 parts and VD37 parts of VC and 500 parts of VC.
The mineral comprises the following components in parts by weight: 3 parts of NaF, 1 part of KI and CoCl2·6H270 portions of O and CuSO4·5H215 portions of O and FeSO4·H2100 parts of O, ZnSO4·H2O60 parts, MnSO4·H2O80 parts, MgSO4·7H2O1500 parts, Ca (H)2PO4)2·H24000 parts of O and 150 parts of NaCl.
The prepared feed has the following nutritional levels: 12.81% of crude fat, 43.27% of crude protein and 11.64% of carbohydrate.
Example 4
A functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 41 parts of fish meal, 16 parts of flour, 16 parts of bean pulp, 2 parts of wheat gluten, 2 parts of beer yeast, 4 parts of fish oil, 0.8 part of soybean lecithin, 0.8 part of monocalcium phosphate, 0.8 part of vitamin, 0.8 part of mineral substance, 0.3 part of choline chloride and 3 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:4, and the astaxanthin content of the haematococcus pluvialis is 2% by mass. The content of adenosine in Cordyceps militaris is 500ug/g, and the content of Cordyceps polysaccharide is 20 mg/g.
The vitamins comprise the following components in parts by weight: VB110 parts and VB231 parts and VB611 parts and VB120.08 parts of VK36 parts of inositol 600 parts, 30 parts of pantothenic acid, 150 parts of nicotinic acid, 15 parts of folic acid, 1 part of VH and VD32 parts of VC 150 parts.
The mineral comprises the following components in parts by weight: 1.5 parts of NaF, 0.4 part of KI and CoCl2·6H2O35 parts and CuSO4·5H2O6 parts, FeSO4·H2O75 parts, ZnSO4·H2O25 parts and MnSO4·H2O55 parts, MgSO4·7H2O1100 parts, Ca (H)2PO4)2·H2O1500 parts and NaCl 60 parts.
The prepared feed has the following nutritional levels: 11.67% of crude fat, 41.23% of crude protein and 13.17% of carbohydrate.
Example 5
A functional feed for improving growth and immunity of micropterus salmoides comprises the following components in parts by weight: 48 parts of fish meal, 23 parts of flour, 18 parts of soybean meal, 4 parts of vital gluten, 4 parts of beer yeast, 4 parts of fish oil, 1.5 parts of soybean lecithin, 1.5 parts of monocalcium phosphate, 1.5 parts of vitamin, 1.6 parts of mineral substances, 0.8 part of choline chloride and 8 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:6, and the astaxanthin content of the haematococcus pluvialis is 4% by mass. The content of adenosine in Cordyceps militaris is 1000ug/g, and the content of Cordyceps polysaccharide is 60 mg/g.
The vitamins comprise the following components in parts by weight: VB111 parts and VB233 parts, VB624 portions and VB120.2 part of VK311 parts of inositol 900 parts, 65 parts of pantothenic acid, 250 parts of nicotinic acid, 25 parts of folic acid, 1.4 parts of VH and VD36 parts and VC 300 parts.
The mineral comprises the following components in parts by weight: 2.5 parts of NaF, 0.9 part of KI and CoCl2·6H260 portions of O and CuSO4·5H2O12 parts, FeSO4·H2O90 parts, ZnSO4·H2O55 parts and MnSO4·H2O70 parts, MgSO4·7H2O1400 parts, Ca (H)2PO4)2·H23500 parts of O and 120 parts of NaCl.
The prepared feed has the following nutritional levels: 11.33% of crude fat, 41.58% of crude protein and 13.37% of carbohydrate.
Comparative example 1
In the feed of the embodiment, the immunopotentiator component is cancelled on the basis of the embodiment 2, other components and contents are unchanged, and the specific feed components and contents are as follows:
the micropterus salmoides feed comprises the following components in parts by weight: 45 parts of fish meal, 20 parts of flour, 16.3 parts of soybean meal, 3 parts of vital gluten, 3 parts of beer yeast, 5 parts of fish oil, 1 part of soybean lecithin, 1 part of monocalcium phosphate, 1.2 parts of vitamin, 1 part of mineral substance and 0.5 part of choline chloride.
The vitamins comprise the following components in parts by weight: VB19.6 parts and VB232 parts and VB620 parts and VB120.1 part of VK310 parts of inositol 800 parts, pantothenic acid 60 parts, nicotinic acid 200 parts, folic acid 20 parts, VH1.2 parts and VD35 parts and VC200 parts.
The mineral comprises the following components in parts by weight: NaF 2 parts, KI 0.8 parts and CoCl2·6H 250 portions of O and CuSO4·5H2O10 parts, FeSO4·H2O80 parts, ZnSO4·H 250 portions of O and MnSO4·H2O60 parts, MgSO4·7H2O1200 parts, Ca (H)2PO4)2·H23000 parts of O and 100 parts of NaCl.
The prepared feed has the following nutritional levels: 12.61% of crude fat, 42.02% of crude protein and 12.34% of carbohydrate.
An 8-week culture experiment was used to evaluate the effect of the composite immunopotentiator on micropterus salmoides. The control group used the feed of comparative example 1, the experimental group used the feed of example 2, and the feeding period was 8 weeks. During the experimental period of cultivation, 8 am each day: feeding twice at 00 and 17:00 pm for satiation, and recording food intake every day. After 8 weeks of culture test, weighing the fishes for calculating the weight gain rate and the specific growth rate; taking liver tissue for antioxidant enzyme activity and tissue morphology analysis (HE staining); taking intestinal tissues for digestive enzyme activity analysis; muscle tissue was taken for measurement of astaxanthin accumulation.
The experimental results show that the growth performance, the survival rate and the feed utilization efficiency of the micropterus salmoides are obviously improved by adding the compound immunopotentiator into the micropterus salmoides feed (Table 1). Experiments show that the growth performance, the survival rate and the feed utilization rate of the micropterus salmoides can be obviously improved while the high nutritional requirement of the micropterus salmoides is maintained, the culture period can be shortened, the cost is saved, the yield can be greatly improved, and the economic income of farmers is increased.
TABLE 1 Effect of Complex immunopotentiators on growth Performance, feed efficiency and survival Rate of Micropterus salmoides
aP、bP<0.05, there was a significant difference between groups
The experimental result shows that the activity of the antioxidant enzyme of the micropterus salmoides can be obviously improved by feeding the micropterus salmoides with the feed containing the compound immunopotentiator (Table 2). The result shows that the invention can enhance the oxidation resistance of the micropterus salmoides, improve the capacity of resisting the external environment pressure in the culture process, help to reduce the susceptibility of the micropterus salmoides to pathogenic microorganisms and improve the disease resistance.
TABLE 2 Effect of Complex immunopotentiators on antioxidant enzyme Activity of Micropterus salmoides
SOD, superoxide dismutase, U mg-1A protein; GSH-PX glutathione peroxidase, U mg- 1A protein; total antioxidant capacity of T-AOC, mmol g-1A protein; MDA malondialdehyde, nmol mg-1protein
aP、bP<0.05, there was a significant difference between groups
The experimental result shows that the compound additive can obviously improve the digestive enzyme activity of the intestinal tract of the micropterus salmoides (Table 3). The result shows that the invention can improve the utilization efficiency of the micropterus salmoides on the feed protein and fat, is beneficial to reducing the feed coefficient and saving the feed cost. Meanwhile, the emission of nitrogen in the residual bait to the environment is greatly reduced, and the ecological environment and the water environment are protected.
TABLE 3 Effect of Complex immunopotentiators on the digestive enzyme Activity of Micropterus salmoides
LPS: lipase, U g protein-1(ii) a AMS: amylase, U mg protein-1(ii) a PEP: pepsin, U mgprotein-1
aP、bP<0.05, there was a significant difference between groups
Experimental results show that the addition of the composite immunopotentiator significantly improves the accumulation of astaxanthin in fish muscle (table 4). The result shows that the invention can improve the content of astaxanthin in fish muscle and improve the quality and value of the fish muscle. People eat the micropterus salmoides rich in functional active ingredients, and the immunity and certain antioxidation and anti-aging effects can be improved.
TABLE 4 Effect of Complex immunopotentiators on astaxanthin content in muscle of Perch California
Experimental results found that the composite immunopotentiator was able to significantly reduce the deposition of fat in liver tissue (fig. 1). The results show that the invention is beneficial to improving the liver health of the micropterus salmoides, improving the transportation capability of liver fat and reducing fat deposition. The invention greatly relieves the problem of liver damage caused by the existing artificial compound feed in the market, and the compound immunopotentiator has important economic value.
Claims (8)
1. The functional feed for improving the growth and immunity of micropterus salmoides is characterized by comprising the following components in parts by weight: 40-50 parts of fish meal, 15-25 parts of flour, 15-20 parts of bean pulp, 1-5 parts of wheat gluten, 1-5 parts of beer yeast, 3-6 parts of fish oil, 0.5-2 parts of soybean lecithin, 0.5-2 parts of calcium dihydrogen phosphate, 0.5-2 parts of vitamin, 0.5-2 parts of mineral matter, 0.2-1 part of choline chloride and 2-10 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, wherein the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1:3-1: 7.
2. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 1, wherein the feed comprises the following components in parts by weight: 45 parts of fish meal, 20 parts of flour, 16.3 parts of soybean meal, 3 parts of vital gluten, 3 parts of beer yeast, 5 parts of fish oil, 1 part of soybean lecithin, 1 part of monocalcium phosphate, 1.2 parts of vitamin, 1 part of mineral substance, 0.5 part of choline chloride and 3 parts of immunopotentiator; the immunopotentiator comprises haematococcus pluvialis and cordyceps militaris, wherein the mass ratio of the haematococcus pluvialis to the cordyceps militaris is 1: 5.
3. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 1, wherein the vitamins comprise the following components in parts by weight: VB19-12 parts of VB230-35 parts of VB610-25 parts of VB120.05-0.3 part and VK35-12 parts of inositol 500-31-7 parts and 500 parts of VC 100-.
4. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 3, wherein the vitamins comprise the following components in parts by weight: VB19.6 parts and VB232 parts and VB620 parts and VB120.1 part of VK310 parts of inositol 800 parts, pantothenic acid 60 parts, nicotinic acid 200 parts, folic acid 20 parts, VH1.2 parts and VD35 parts and VC200 parts.
5. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 1, wherein the mineral comprises the following components in parts by weight: 1-3 parts of NaF, 0.3-1 part of KI and CoCl2·6H230-70 parts of O and CuSO4·5H25-15 parts of O and FeSO4·H270-100 parts of O, ZnSO4·H220-60 parts of O and MnSO4·H250-80 parts of O and MgSO4·7H21500 portions of O1000-2PO4)2·H24000 parts of O1000-.
6. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 5, wherein the mineral comprises the following components in parts by weight: NaF 2 parts, KI 0.8 parts and CoCl2·6H250 portions of O and CuSO4·5H2O10 parts, FeSO4·H2O80 parts, ZnSO4·H250 portions of O and MnSO4·H2O60 parts, MgSO4·7H2O1200 parts, Ca (H)2PO4)2·H23000 parts of O and 100 parts of NaCl.
7. The functional feed for improving growth and immunity of micropterus salmoides according to claim 1, wherein the haematococcus pluvialis contains 2-4% by mass of astaxanthin.
8. The functional feed for improving the growth and immunity of micropterus salmoides according to claim 1, wherein the content of adenosine in the cordyceps militaris is 500-1000ug/g, and the content of cordyceps polysaccharide is 20-60 mg/g.
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