CN111471596B - Bipoloid helminthosporium umbiliciformis with herbicidal activity and application thereof - Google Patents

Abstract

The invention discloses a bipolor helminthosporium with herbicidal activity and application thereof. A Bipolaris bicolor (Bipolar bicolor (Mitra) Shoem) SYNJC-2-2, collection unit: china general microbiological culture Collection center, preservation date: 9/2/2019, and the accession number is: CGMCC No. 18160. According to the crop safety test, the bipolarity helminthosporium synJC-2-2 can be safely used in crop fields, tea gardens and traditional Chinese medicine gardens of cotton, rape, sesame, tobacco, vegetables and the like, and is used for controlling gramineous weeds of goosegrass, green bristlegrass, tarragon and the like. This indicates that the bipolaris paregorica SYNJC-2-2 has the potential of being developed into a biological herbicide for preventing and killing gramineous weeds such as goosegrass.

Description

Bipoloid helminthosporium umbiliciformis with herbicidal activity and application thereof

Technical Field

The invention belongs to the field of agricultural biological preparations, and relates to diplocarpus peregrinus with herbicidal activity and application thereof.

Background

China is a big agricultural country with thousands of years of history, and the development relationship of agriculture is strong between the civilian and the country. However, the random growth of weeds in farmlands restricts the development of modern agriculture and reduces the yield of grains. China has wide land and various weed species, wherein 255 kinds of weeds in farmland cause about 10 kinds of serious harm, and the 10 kinds of harmful weeds account for about 40 kinds of gramineae. Goosegrass, green bristlegrass herb, moleplant seed, barnyard grass, crab grass, beckmark grass and the like are weeds with serious harm to farmlands in China and compete for sunlight, water and nutrients with crops. Common weeding methods include artificial weeding, chemical weeding, mechanical weeding, and the like. Chemical weeding is the most economical and efficient weeding method at present, but long-term use of chemical herbicides also brings many negative problems, including environmental pollution, pesticide residues, weed resistance and the like. With the rapid development of economy and the increasing improvement of the living standard of people, people pay more attention to the concept of green sustainable development, so that the development of the biological herbicide for preventing and killing weeds is a new way with great potential. At present, no effective biological herbicide is available in China for preventing and killing the gramineous weeds, and the strain has the potential of preventing and killing the harmful weeds, so that huge economic benefit and social value can be brought by developing the strain into the biological herbicide undoubtedly.

The goosegrass (with the scientific name of Eleusine indica (L.) Gaertn.) is also called cricket grass and oil hyacinth grass, is mainly distributed in provinces of south and north China and temperate and tropical areas all over the world, is one of ten malignant weeds in the world, and harms economic crops such as cotton, beans, potatoes, vegetables, orchards, tea gardens and the like. It has the characteristics of high growth speed, strong tillering capability, high maturing rate, cold resistance, barren resistance and the like. Its root system is developed, and it is difficult to prevent and remove, and often forms single dominant species community, so that it seriously harms the normal growth of economic crops.

About 30 kinds of microorganisms, which have been commercialized or are about to be commercialized for the development of the bioherbicide, are available, and there are nearly 20 kinds, in which the kinds having the development potential of fungi are mainly concentrated in several genera, including Colletotrichum (Colletotrichum), Phytophthora (Phytophthora), Fusarium (Fusarium), Alternaria (Alternaria), sporotrichum (Cercospora), Phytophthora (Phytophthora), Puccinia (Puccinia), aleuromyces (Entyloma), chitosonia (Ascochyta), Sclerotinia (sclerotiorum), and the like, according to the characteristics of the bioherbicide. There are many cases of successful weeding with fungi in the world at present, including introducing rust fungi (pucciniachondrilina) from europe in australia in 1971 to control cornfield pink bud lamp bas (Chondrilla juncea L.); in 1979, fusarium graminearum (F. umorobanche) is separated from a Hami quality inspection station in Xinjiang in China and is prepared into a biocontrol microbial inoculum 'F798', so that the fusarium graminearum can be effectively prevented and killed, and is harmless to other economic crops; 1981 Devine registered in the United states, a Malva verticillata proprietary collembola (colle. sp. Malvae) agent commercially available from Philom BioS, 1992, using a Phytophthora palmi (Phytophorapalmivora Butl.) preparation for soil treatment against Morenia longissima sites in citrus orchards was used to control Malva verticillata and Abutilon.

At present, the bipolaris viridis, maize bipolaris, millet bipolaris and millet bipolaris have patents related to biological herbicides in the genus of bipolaris, and there is no report on the bipolaris viridis or the bipolaris viridis applied to the environment such as crop fields, traditional Chinese medicine gardens and tea gardens to control grass family weeds.

Disclosure of Invention

The invention aims to screen out the bicolor helminthosporium strain which has the characteristics of green sustainability, high efficiency, low cost, safety to crops and the like and is used for biological weeding against gramineous weeds such as goosegrass, green bristlegrass, large crabgrass, Gentiana mollissima, barnyard grass, pennisetum alopecuroides and the like in crop fields such as tobacco, cotton, sesame, rape, vegetables and the like and tea gardens and traditional Chinese medicine gardens.

In order to achieve the above purpose, the invention discloses a bipigment planocopterosporium strain, a screening method and application thereof, and adopts the following technical scheme:

a Bipolaris bicolor (Bipolaris bicolor) SYNJC-2-2, collection unit: china general microbiological culture Collection center, preservation date: 9/2/2019, and the accession number is: CGMCC No. 18160.

The application of the bipolarity helminthosporium synJC-2-2 in preventing and removing grassy weeds is disclosed; preferably, the bipolarity helminthosporium syn jc-2-2 is applied to preventing and killing gramineous weeds in farmlands, traditional Chinese medicine gardens and tea gardens; the farmland is a tobacco field, a rape field, a cotton field and a vegetable field; the grassy weeds are preferably any one or more of green bristlegrass herb, goosegrass herb, tarragon, pennisetum alopecuroides, elymus foenum-graecum, large crabgrass, barnyard grass and bluegrass, and further preferably any one or more of the goosegrass herb, the goosegrass herb and the tarragon.

In the application, the spore suspension of the SYNJC-2-2 strain is further preferably sprayed on target weeds, or mycelium of the strain is utilized to prepare various formulations for preventing and killing the target weeds; the spore concentration of the spore suspension of the SYNJC-2-2 strain is more than 10⁴one/mL.

The invention relates to application of Bipolaris bicolor (Bipolaris bicolor) SYNJC-2-2 in preparation of biological herbicides.

A herbicide composition comprises the Bipolaris bicolor (Mitra) Shoem SYNJC-2-2 and an agriculturally and pharmaceutically acceptable auxiliary agent.

Preferably, the Verticillium bicolor (Bipolaris bicolor) SYNJC-2-2 is one selected from a SYNJC-2-2 culture, a SYNJC-2-2 living cell obtained by culture, a SYNJC-2-2 culture filtrate or a composition of the SYNJC-2-2 cell and the culture filtrate.

As a preferred aspect of the present invention, the adjuvant is selected from one or more of a surfactant, a stabilizer and a carrier.

As a preferred of the present invention, the surfactant is selected from one or more of tween 20, tween 80, rapeseed oil or lecithin; the stabilizer is selected from an antioxidant or a pH regulator; the antioxidant is preferably acacia, gelatin, etc.; the pH regulator is preferably calcium carbonate, phosphoric acid and the like; the carrier is selected from various plant materials or ores; the plant material is preferably one or more of rice hull powder, wheat bran, wood sawdust, rye grass powder, wheat straw and corn straw; the ore is preferably talcum powder, diatomite or kaolin.

Preferably, the herbicide composition is prepared into dosage forms such as aerosol, water aqua, microemulsion, powder, microcapsule, suspending agent, granules and the like, and is preferably processed into dosage forms which can be sprayed for use.

In the herbicidal composition of the present invention, the conidium concentration and the active substance content of the strain SYNJC-2-2 are adjusted depending on the formulation and the mode of use, and the concentration of the active substance used is preferably more than 5X 10⁴Spore suspension per mL.

The herbicidal composition of the present invention can be used in combination with other suitable strains or chemical herbicides to broaden the herbicidal spectrum or to reduce the amount of chemical herbicide used.

The main advantages and positive effects of the invention are as follows:

1. the bifoloid Verticillium elegans specialized microbial inoculum used for biological weeding can be safely selected between crops and gramineous weeds. The herbicide is environment-friendly, is beneficial to popularization of green food and organic agriculture, and is low in cost, pollution-free and safe to crops. Of the 55 plants of 22 families tested, 5 were sensitive to P.bicolor, 4 were sensitive, 2 were slightly sensitive and 44 were insensitive, with the sensitive plants being mostly concentrated in the Poaceae family and insensitive to other plants such as the Compositae, Convolvulaceae, Moraceae, Amaranthaceae, Euphorbiaceae, Rosaceae, Vitaceae, Commelinaceae, Raynariaceae, Araliaceae, Sterculiaceae, Lionaceae, Pedaliaceae, Cruciferae, etc. From the aspect of morphology, spores mainly infect leaves of gramineous plants, because the leaves are parallel veins and thin, the spores are easy to generate infection structures such as adhesion cells and the like after contacting and quickly diffuse and penetrate the leaves, and weeds of compositae, Convolvulaceae, Euphorbiaceae, Caryophyllaceae, Scrophulariaceae and the like have wide leaves, have developed reticular vein infection structures and are not easy to penetrate, so the weeds are not easy to infect.

2. The invention is used for biological weeding, utilizes living organisms which are directly collected in natural environment and are natural pathogenic bacteria of target weeds generated in target crop fields and environments, has no ecological risk in the using process because the living organisms exist in the environment, and has strong specialization of the strain, high safety to crops and other non-target plants, no residual hazard in the environment, rapid decomposition and degradation after the death of the bacteria, recyclable organic matters of degradation products and no pollution. Therefore, the strain is safe to the environment and can be used for preventing and controlling target weeds in the production process of green and organic agricultural products.

3. The prevention and control effect of the strain on target weeds can reach more than 90%, and particularly the prevention and control effect of plants in a seedling stage is better and obvious.

4. According to the crop safety test, the bipolarity helminthosporium synJC-2-2 has higher sensitivity to corn and soybean, and can be safely used in crop fields such as cotton, rape, sesame, tobacco and vegetables, tea gardens and traditional Chinese medicine gardens. And can be used for controlling the grassy weeds such as the field goosegrass herb, the green bristlegrass herb, the Gentiana leptostachya and the like. This again indicates that the bipolaris paregorica SYNJC-2-2 has the potential of being developed into a biological herbicide for preventing and killing gramineous weeds such as eleusine indica and the like.

Drawings

FIG. 1 is a colony morphology of a strain of SynJC-2-2 Bicoloris Panaxacum umbilicifolius;

FIG. 2 is a conidia morphogram of a strain of SynJC-2-2 Helminthosporium bicolor;

FIG. 3 is a gel electrophoresis chart of a strain of NJC-2-2 two-color Helminthosporium umbilicifolius, wherein 1 in FIG. 3 is an ITS sequence band, and 2 is a GPDH sequence band.

Biological material preservation information

SYNJC-2-2, classified and named as the Bipolaris bicolor, the collection unit: china general microbiological culture Collection center; the address Beijing, Chaoyang, district, Beichen Xilu No.1 institute No. 3, institute of microbiology, China academy of sciences; the preservation date is as follows: year 2019, month 9, and day 2; the preservation number is as follows: CGMCC No. 18160.

Detailed Description

Example 1

The new strain is obtained by recording the occurrence conditions of weeds in tea gardens of main tea production areas such as Jiangsu, Zhejiang and Anhui, collecting seriously-infected weed plants, recording relevant information such as plant disease symptoms and the like, bringing the plants back to a laboratory, carrying out separation research on parasitic fungi by utilizing the experimental technology of plant pathology, and carrying out pathogenicity verification strictly according to the koch formula rule.

The bipolarity cord worm fungus SYNJC-2-2 belongs to the kingdom Fungi (Fungi), Ascomycota (Ascomycota), Ascomycetes (Dothideomycetes), Geosporaceae (Pleosporomytidae), Geosporales (Pleosporales), Geosporaceae (Pleosporaceae), Helminthosporium (Bipolaris), Bipolaris bicolor (Bipolar) Shoem) grows faster on potato glucose medium, the diameter of the colony is 68-72 mm after culturing for 4 days under dark condition at 28 ℃, the colony is grey green, compact hyphae, the edge of the colony is white, and the back of the colony is yellow brown. FIG. 1 shows the colony morphology of Helminthosporium bicolor cultured in the dark at 28 ℃ for 4 days. Conidiophores are unigenous or fasciculate, dark brown, separated and verrucous and protuberant at sporulation sites. Conidiophore cluster grows on the top of conidiophore, is upright or curved, cylindrical, slightly wide in the middle, blunt and round at two ends, has 5-8 (mostly 6) false septa, and has size of 45-80 μm × 10-15 μm. The conidium has lighter color at two ends and deeper septum. FIG. 2 shows the conidiophores and conidiophores of Helminthosporium bicolor.

Firstly, the pathogenic symptoms of the dipterous chrysosporium strain (Bipolaris bicolor) SYNJC-2-2 natural condition infection of the goosegrass are that the blades of the goosegrass plants can be seen with scabs with different sizes, the blades are yellowed, the plants are withered, and black dotted scabs can be seen.

Shearing the tissue block (about 5 mm. times.5 mm) at the junction of diseased leaves with scissors, sterilizing the surface with 75% ethanol and 2% sodium hypochlorite solution, washing with sterile water, and washing with sterile filter paper. Inoculating the small tissue blocks on a PDA culture medium, culturing at 28 ℃ in the dark, observing once every 12h until fine hyphae grow around the tissue blocks, picking the hyphae by using an inoculating needle, transferring to a new PDA culture medium, and performing single spore isolation and purification culture on the separated strains.

Placing the bacterial mass with the diameter of 5mm on the isolated leaf of the moist Eleusine indica by using a puncher with the diameter of 5mm, and culturing for four days in an incubator with the light and dark alternation of 28 ℃ and 12h/12 h. Observing the injury degree of the leaves, if the leaves generate diseases and the symptoms are similar to the collected symptoms of the diseased leaves, determining the disease-causing strains of the eleusine indica, storing the disease-causing strains on a slant culture medium, injecting sterile liquid paraffin, and placing the mixture at 4 ℃ for later use.

The shapes of conidia and conidiophores are observed under an optical microscope, and meanwhile, the sizes of the conidia are measured by photomicrographs. Pathogenic bacteria are identified according to morphological characteristics described in Chinese journal of fungi, handbook of fungi identification and related documents. The description of the strain is basically consistent with that of the helminthosporium Bipolaris bicolor with the strain number of ACCC38218 described by Guo Yujie and the strain is identified as the helminthosporium Bipolaris bicolor (Mitra) Shoem. The description is as follows: the colony is gray green, the hyphae are compact and velvet, the edge of the colony is white, and the back of the colony is yellow brown. The hypha is yellowish brown with septum. Conidiophores are unigenous or fasciculate, dark brown, separated and verrucous and protuberant at sporulation sites. Conidiophore cluster grows on the top of conidiophore, is upright or curved, cylindrical, slightly wide in the middle, blunt and round at two ends, has 5-8 (mostly 6) false septa, and has size of 45-80 μm × 10-15 μm. The conidium has lighter color at two ends and deeper septum.

The fungus gDNA extraction kit is adopted for extraction, rDNA ITS sequences and GPDH sequences are respectively utilized for PCR amplification, and the universal primers are ITS1(5'-TCCGTAGGTGAACCTGCGG-3') shown as SEQ ID NO:3, ITS4 (5'-TCCTCCGCTTATTGATATGC-3') shown as SEQ ID NO:4, gpd1(5'-CAACGGCTTCGGTCGCATTG-3') shown as SEQ ID NO:5 and gpd2(5'-GCCAAGCACTTGGTTGTGC-3') shown as SEQ ID NO: 6. And (3) recovering the amplification product through 1% agarose gel electrophoresis, connecting the amplification product to a pMD19-T vector, transforming escherichia coli, screening through blue white spots, selecting white colonies, and shaking to obtain a bacterial liquid. PCR amplification is carried out by using bacteria liquid, detection is carried out by an electrophoresis and a gel analyzer, a single bright band is displayed on 1% agarose gel, as shown in the figure, 1 in the figure is an ITS amplification sequence, the size of a target band is about 600bp, 2 is a GPDH amplification sequence, and the size of the target band is about 650 bp. And (3) sequencing the bacterial liquid by entrusting Shanghai bio-corporation, editing a measured result by using DNAstar and BioXM, cutting off sequences at the front end of an upstream primer and the tail end of a downstream primer, and performing homology comparison on the obtained complete ITS sequence or GPDH sequence and a related sequence of a nucleic acid database in Genbank. After amplification sequencing comparison, the homology of the ITS amplification sequence and a model strain Bipolaris bicolor (MF490804.1) is 99%, the homology of the GPDH amplification sequence and the model strain Bipolaris bicolor (MF490827.1) is 99%, and the strain is determined to be Helminthosporium bicolor. The ITS amplification sequence is shown in SEQ ID NO.1, and the GPDH amplification sequence is shown in SEQ ID NO. 2.

The pathogenic symptoms of spore suspension prepared by spores produced by culturing the bipolaris paregori SYNJC-2-2 to be sprayed on the goosegrass are as follows: dark brown spots appear on the leaves at the early stage of the onset of the disease, the disease area of the leaves is enlarged at the middle stage of the onset of the disease, the leaves are soft, the color is deepened, and the leaves are withered and withered at the later stage of the disease. And (3) re-separating pathogenic bacteria from diseased leaves, wherein the shapes of the separated pathogenic bacteria colonies and spores are consistent with the shape of the sprayed bicolor panumbilicus helminthosporium strain, and the separated pathogenic bacteria colonies and spores conform to the Koehler's rule.

The onset symptoms under the above conditions are similar to those of naturally collected disease strains.

The bipolarity cord-flush spore strain SYNJC-2-2 is separated from eleusine indica, but has broad-spectrum pathogenicity of gramineous weeds, and also has another advantage that safe selection can be made between crops and target weeds, so that the bipolarity cord-flush spore strain SYNJC-2-2 has the same specificity as a chemical herbicide aromatic phenoxy propionic acid herbicide and is safe to the environment.

They can be identified by their morphological characteristics as Ascomycota (Ascomycota), Ascomycota (dothieomycetes), Geosporales (Pleosporomycetidae), Geosporales (Pleosporales), Geosporaceae (Pleosporaceae), Deuterorhabdus (Bipolaris), Deuterorhabdus bicolor (Mitra) Shoem. The special strain mainly infects gramineous weeds such as eleusine indica, setaria viridis, pennisetum alopecuroides, phyllostachys leptostachya, digitaria sanguinea and barnyard grass, and has high immunity to main economic crops such as tobacco, cotton, sesame, rape, vegetables and the like. Of the 55 plants of 22 families tested, 5 were sensitive to bipolarity helminthosporium, 4 were sensitive, 2 were slightly sensitive and 44 were insensitive, with the sensitive plants mostly being concentrated in the grass family and being insensitive to other plants of other families such as the feverfew, convolvulaceae, sangaceae, amaranthaceae, euphorbiaceae, rosaceae, vitiaceae, dayflower, alliaceous, araliaceae, sterculiaceae, polygonaceae, solanaceae, sessile, leguminosae, cruciferae, etc. Therefore, the strain can be used in fields of tobacco, rape, sesame, cotton and vegetable crops, tea gardens, orchards and traditional Chinese medicine gardens for controlling grassy weeds such as goosegrass, green bristlegrass, Gentiana mollissima and the like.

Example 2

The bipolarity helminthosporium syncjc-2-2 is used for biological weeding, and the specific application method is that the strain is inoculated in a goosegrass juice culture medium, conidia are generated to prepare a spore suspension, and the spore suspension is sprayed in the field or a weed emergence place, and the using dosage is 10⁴～10⁵one/mL.

The culture method comprises the following steps: the bipoloid helminthosporium is inoculated in a goosegrass juice culture medium, the culture is carried out for 5 days at 28 ℃ under a dark condition, and then the culture is placed under a black light lamp to induce sporulation for about 10 days, so that the strain can produce a large amount of spores.

Culture medium of goosegrass juice (1L)

Herba Eleusines Indicae leaf 30g

Agar 20g

Distilled water to constant volume of 1L

At present, no report about the application of the bipolaris umbiliciformis in biological herbicides exists at home and abroad, and the research on the aspect provides a new excellent way for preventing and killing the eleusine indica. The weeding composition can be matched with other suitable chemical herbicides for use, so that the dosage of the chemical herbicides is reduced, and the pollution to the environment is reduced.

According to the needs, the weeding composition of the invention can use surface active agent, stabilizer and carrier and other auxiliary agents, the surface active agent such as Tween 20, Tween 80 and the like, the stabilizer can use antioxidant or regulator and the like, and the carrier can use plant materials or ore and the like. Plant materials such as wheat bran, rice hull powder, rye grass powder, wheat straw, rice straw, sawdust, etc. Minerals such as kaolin, talc, diatomaceous earth, etc.

Example 3

Inoculating strain SYNJC-2-2 to goosegrass juice culture medium, culturing in dark at 28 deg.C for 5 days, inducing spore production under black light for about 10 days, eluting spores with sterile water, filtering hypha with a layer of mirror paper to obtain spore suspension, counting with blood counting plate, gradient diluting to adjust the concentration of spore suspension to 5 × 10⁵2/mL, 5X 10⁴5X 10 units/mL³5X 10 units/mL²5X 10 units/mL¹Five concentration gradients per mL, 0.02% tween 80 was added. Meanwhile, 0.02% tween 80 was added to sterile water as a control group. And culturing the goosegrass seedlings in a flowerpot with the diameter of 9cm, and uniformly spraying the spore suspension or sterile water containing 0.02% Tween 80 on the surface of the seedlings by using a handheld sprayer when the goosegrass seedlings grow to the 3-4 leaf stage. Each treatment group treats four pots of eleusine indica seedlings with consistent growth vigor, moisture is kept at 28 ℃ for 48 hours, and after one week of treatment, the concentration of spore suspension is more than 5 multiplied by 10⁴Plants in the treated group per mL were severely diseased and completely dead.

Example 4

Seeds of test plants (see tables 1 and 2) were sown in pots having a diameter of 9cm, and set to 4 replicates, and cultivated in a greenhouse at 28 ℃, and when the test plants reached the 3-4 leaf stage, inoculation was performed as in example 2, with 4 pots for each plant, and sterile water containing 0.02% tween 80 was sprayed as a control group. The safety test result shows that the SYNJC-2-2 strain has stronger pathogenicity on corn, pathogenicity on soybean and no pathogenicity on cotton, tobacco, peanut, broad bean, sesame, rape, eggplant, tomato, tea tree and the like. The results of the weed control spectrum test show that SYNJC-2-2 has strong pathogenicity on goosegrass, tarragon, green bristlegrass and pennisetum alopecuroides, has certain pathogenicity on hispid arthraxon, large crabgrass, barnyard grass and ryegrass, and has no pathogenicity on solidago canadensis, eupatorium adenophorum, sambucus samara, dandelion, quassia, ageratum thistle, horseweed herb, sticktight, cocklebur, dayflower, Indian mockstrawberry herb, Japanese soybean, Japanese cayratia, morning glory and the like. Therefore, the SYNJC-2-2 strain can be applied to crop fields of cotton, tobacco, peanut, sesame, rape and the like, tea gardens and Chinese medicinal material fields, and can be used for preventing and controlling grassy weeds of large crabgrass, barnyard grass, elymus, phyllostachys scaber, eleusine indica, green bristlegrass and the like.

TABLE 1 susceptibility testing of crops to the strain SYNJC-2-2Bipolaris bicolor

Note: the disease indexes of the same plants at different concentrations are shown in the table, the values are mean values +/-standard deviation, and the difference is marked by lower case letters (P < 0.05).

TABLE 2 sensitivity testing of weeds to the Strain SYNJC-2-2Bipolaris bicolor

Note: the disease indexes of the same plants at different concentrations are shown in the table, the values are mean values +/-standard deviation, and the difference is marked by lower case letters (P < 0.05).

Claims (12)

1. A Bipolaris bicolor (Bipolaris bicolor) SYNJC-2-2, collection unit: china general microbiological culture Collection center, preservation date: 9/2/2019, and the accession number is: CGMCC number 18160.

2. The use of the helminthosporium diplodium syn jc-2-2 of claim 1 for controlling grassy weeds, wherein the grassy weeds are any one or more of goosegrass herb, taraxacum leptostachya, setaria viridis, pennisetum alopecuroides, hispid axon, large crabgrass, barnyard grass and ryegrass.

3. The use according to claim 2, characterized in that the use of the bipolarity helminthosporium SYNJC-2-2 for controlling grassy weeds in farmlands, traditional Chinese medicine gardens and tea gardens; the farmland is paddy field, tobacco field, cotton field, peanut field, cowpea field, sesame field, rape field, pepper field, eggplant field, and tomato field, and the traditional Chinese medicine field is radix Isatidis plantation and flos Lonicerae plantation.

4. The use according to claim 2 or 3, wherein spore suspension of said SYNJC-2-2 strain is sprayed on target weeds, or mycelium of said strain is used to prepare various formulations for controlling target weeds; the spore concentration of the spore suspension of the SYNJC-2-2 strain is more than 10⁴one/mL.

5. Use of helminthosporium diplodium (Bipolaris bicolor) SYNJC-2-2 according to claim 1 for the preparation of a biological herbicide.

6. A herbicidal composition characterized by comprising the helminthosporium diplodiella (Bipolaris bicolor) SYNJC-2-2 of claim 1 and an agriculturally pharmaceutically acceptable adjuvant.

7. The herbicidal composition of claim 6, wherein said helminthosporium diplodiella (Bipolaris bicolor) SYNJC-2-2 is selected from the group consisting of a culture of SYNJC-2-2, cultured SYNJC-2-2 living cells, a combination of SYNJC-2-2 cells and a filtrate of said culture.

8. A herbicidal composition according to claim 6, characterized in that the adjuvant is selected from one or more of the group consisting of surfactants, stabilizers and carriers.

9. A herbicidal composition according to claim 8, wherein the surfactant is selected from one or more of Tween 20, Tween 80, rapeseed oil or lecithin; the stabilizer is an antioxidant or a pH regulator; the antioxidant is preferably gelatin and acacia; the carrier is selected from various plant materials or ores.

10. A herbicidal composition according to claim 9, characterized in that the pH adjusting agent is preferably calcium carbonate and/or phosphoric acid; the plant material is selected from one or more of rice hull powder, wheat bran, wood sawdust, rye grass powder, wheat straw and corn straw; the ore is selected from talcum powder, diatomite or kaolin.

11. A herbicidal composition according to claim 9, wherein the herbicidal composition is formulated as an aerosol, aqueous solution, microemulsion, powder, microcapsule, suspension or granule.

12. A herbicidal composition according to claim 11, wherein said herbicidal composition is formulated for sprayable use.

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