CN106701624A - Fructus lycii root rot-resistant bacillus tequilensis and application thereof - Google Patents
Fructus lycii root rot-resistant bacillus tequilensis and application thereof Download PDFInfo
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Abstract
The invention discloses fructus lycii root rot-resistant bacillus tequilensis. The fructus lycii root rot-resistant bacillus tequilensis is classified and named as bacillus tequilensis GQJK2, which is collected in the China General Microbiological Culture Collection Center (CGMCC for short, located in the Institute of Microbiology, Chinese Academy of Sciences, #3, First Yard, Beichen Road West, Chaoyang District, Beijing) on November 10th, 2016, and the collection number of the fructus lycii root rot-resistant bacillus tequilensis is CGMCC NO. 13253. The resistant bacillus tequilensis GQJK2 obtained by isolation is relatively high in resistance to fructus lycii root rot, thus providing a novel biocontrol bacterium to biological prevention and control of the fructus lycii root rot and adding a variety to biocontrol preparations.
Description
Technical field
Te Jila bacillus and its application the present invention relates to one plant of antagonism matrimony vine root rot, belong to biotechnology neck
Domain.
Background technology
Matrimony vine is a kind of rare Chinese medicine, and Chinese wolfberry fruit has nutrition and health care and medical value higher, with fine jade treasure ganoderma lucidum, length
White recluse's ginseng, Donga donkey-hide gelatin is simultaneously referred to as " Chinese medicine four is precious ".With expanding economy and the progress of society, people also increasingly recognize
To the value of matrimony vine, people are promoted to carry out extensive product development to matrimony vine, and achieve important achievement.Lycium barbarum is in
State's cultivated area is maximum, and as matrimony vine cultivated area constantly expands, old producing region is gone up year after year with the new producing region matrimony vine root rot incidence of disease
Rise, increasingly seriously, badly influenced local farmers income, have certain destruction to whole industrial chain.Chinese holly
Qi root rot is a kind of system infections disease, and diseased plant root and root neck occur to rot, and cane vascular bundle becomes brown.Disease portion when moist
White or pink mould layer (pathogen) is grown once in a while.Because root-rot, blade diminish, lack gloss, branch is downgraded, and fruit is thin
It is small；Or blade flavescence is wilted, plant is gradually withered when serious.Current people's preventing and treating matrimony vine root rot is mainly sprays insecticide, and matches somebody with somebody
Side's fertilising, the mode of increasing application phosphorus potassium fertilizer.But these methods waste time and energy, it is difficult to from root effective controlling disease generation, and
And it is big for environment pollution, also there is certain injury to health, and biological control method has selectivity and green ring because of it
The advantage of guarantor, is increasingly paid close attention to by people.There is bacillus for cotton wilt, potato sore using comparative maturity
The Biocontrol Effect of the plurality of plant diseases such as the red carninomatosis of scall, apple, root rotof flax, but without matrimony vine root rot biological control
The report of aspect, and the microorganism resource for finding antagonism matrimony vine root rot is crucial.
Te Jila bacillus (Bacillus tequilensis) is a kind in bacillus, can be produced various
Polypeptide antibiotic, can produce antagonism to various plants pathogeny bacterium, and the biocontrol effect about Te Jila bacillus grinds
Study carefully and be concentrated mainly on preventing and treating watermelon blight (CN105695368A), antagonism mulberry fruit core ground cane bacterium (CN105274021A), preventing and treating
Wheat scab (CN104403962A) etc., but do not have the report of the Te Jila bacillus of preventing and treating matrimony vine root rot also at present.
And, often exist between the microbiologic population in Biocontrol microorganism and soil environment and interact, external source antagonism
The introducing of bacterium can cause the composition of crop rhizosphere soils microbiologic population to change, and different plant rhizosphere bacteriums of the same race are determined
Plant and function has larger difference；And, can Biocontrol microorganism survive in soil environment and fast-growth is also real
The key of existing preventive and therapeutic effect.Therefore, screening can have to matrimony vine root rot preventive and therapeutic effect biocontrol bacterial strain have it is particularly significant
Actual application value.
The content of the invention
For above-mentioned prior art, it is an object of the invention to provide one plant of Te Jila bacillus of antagonism matrimony vine root rot
And its application.
To achieve the above object, the present invention uses following technical proposals：
According to the first aspect of the invention, there is provided one plant of Te Jila bacillus of antagonism matrimony vine root rot, its classification life
Entitled Te Jila bacillus (Bacillus tequilensis) GQJK2, is preserved in China micro- on November 10th, 2016
(abbreviation CGMCC, address is biological inoculum preservation administration committee common micro-organisms center：BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3, Institute of Microorganism, Academia Sinica), culture presevation number is CGMCC NO.13253.
The bacterial strain is isolated from matrimony vine rhizosphere soil, and the bacterium colony and thalline of the bacterial strain are characterized as：On LB flat boards
Culture 24h after bacterium colony be in subcircular, milk yellow, bacterium colony flat, the small dentation in edge, dry tack free, colony diameter is 2.57mm.
Thalline is in elongated rod shape, produces gemma, Gram-positive.
The physiological and biochemical property of the bacterial strain is：Oxidizing ferment experiment is negative, and catalase experiment is positive, and citrate is using experiment
The positive, nitrate is negative using experiment, and Starch Hydrolysis experiment is negative, and M-R experiments are negative, and V-P experiments are positive, glucose fermentation reality
The positive is tested, sucrose fermenting experiment is positive, and maltose fermenting experiment is positive, and arabinose fermenting experiment is positive, wood-sugar fermentation experiment
The positive, cellobiose fermenting experiment is negative, and lactose fermentation experiment is negative, and sorbitol fermentation experiment is positive, mannose ferment experiment
It is positive.
According to the second aspect of the invention, there is provided a kind of biological prevention and control agent, its active component is above-mentioned Te Jila bacillus
The tunning of (Bacillus tequilensis) GQJK2.
Preferably, the biological prevention and control agent is liquid microbial inoculum.
Further, Te Jila bacillus (Bacillus tequilensis) GQJK2 contains in the biological prevention and control agent
Amount is more than or equal to 3 × 108cfu/mL。
The present invention also provides the preparation method of above-mentioned biological prevention and control agent, comprises the following steps：The above-mentioned Te Jila gemma of fermentation
Bacillus (Bacillus tequilensis) GQJK2, obtains tunning.
In above-mentioned preparation method, the culture medium that fermentation is used is liquid bean sprouts medium, and its raw material composition is：Moyashi
100g, sucrose 50g, water 1000mL, pH nature；
The preparation method of the liquid bean sprouts medium is：Moyashi boiling water boiling 30min, with four layers of filtered through gauze,
Add sucrose constant volume to 1000mL.
In above-mentioned preparation method, the condition of fermentation is：35-40 DEG C, constant-temperature table culture 20-30h；Preferably, it is 37 DEG C, permanent
Warm shaking table culture 24h.
According to the third aspect of the invention we, there is provided above-mentioned Te Jila bacillus (Bacillus tequilensis)
The application of GQJK2 and/or biological prevention and control agent in matrimony vine root rot is prevented and treated.
In above-mentioned application, the preventing and treating matrimony vine root rot is embodied in the breaking-out for suppressing matrimony vine root rot, reduces the incidence of disease.
According to the fourth aspect of the invention, there is provided above-mentioned Te Jila bacillus (Bacillus tequilensis)
The application of GQJK2 and/or biological prevention and control agent in matrimony vine volume increase is promoted.
The present invention also provides one kind and can prevent and treat matrimony vine root rot, while promoting the product of volume increase, its active component is above-mentioned
Te Jila bacillus (Bacillus tequilensis) GQJK2 or above-mentioned biological prevention and control agents.
Beneficial effects of the present invention：
(1) isolated Te Jila bacillus (Bacillus tequilensis) GQJK2 of the present invention, the bacterial strain pair
Matrimony vine root rot has stronger antagonism, for the biological control of matrimony vine root rot provides new biocontrol microorganisms, increased biological and ecological methods to prevent plant disease, pests, and erosion
The species of preparation.
(2) research shows, under field condition, using Te Jila bacillus (Bacillus of the invention
Tequilensis after) the matrimony vine tree in the bacteria suspension of the GQJK2 plot serious to the morbidity of matrimony vine root rot carries out root irrigation, energy
Substantially suppress the breaking-out of matrimony vine root rot, reduce the incidence of disease；Te Jila bacillus (Bacillus of the invention
Tequilensis) GQJK2 can also improve the upgrowth situation of plant, improve the yield of matrimony vine, and the averagely volume increase of matrimony vine tree is existed
More than 550%.Above result of study shows that the bacillus of antagonism matrimony vine root rot of the invention has to lycium barbarum root rot
There is certain prevention effect.
(3) root rot of different plants, its pathogen is different, and pathogenesis can also have differences.To the root of other plant
Maize ear rot has the bacillus of prevention effect, can not directly deduce that it has prevention effect to matrimony vine root rot.We were testing
Find there is the bacterial strain of antagonistic effect to phytopathogen in flat board face-off experiment, but do not ensure that it in crop field reality in journey
Effect in testing.The result of field experiment also demonstrates that, possesses preferable flat board and stands facing each other the bacterial strain of experimental result, in field experiment
In, obvious inhibition may not also be possessed to matrimony vine Pathogens Causing Root Rot Disease.Therefore, the root rot to other plant has preventing and treating to imitate
For the Te Jila bacillus of fruit, due to the severe living environment in Ningxia, China, bacterial strain can survive and fast-growth into
It is key issue, therefore there may not be prevention effect to matrimony vine root rot.
Brief description of the drawings
Fig. 1：Te Jila bacillus (Bacillus tequilensis) GQJK2 colonial morphology figures；
Fig. 2：The phyletic evolution tree graph of Te Jila bacillus (Bacillus tequilensis) GQJK2.
Specific embodiment
Involved instrument, reagent, material etc. in following embodiments, unless otherwise noted, are existing in the prior art
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Involved experimental technique in following embodiments, inspection
Survey method etc., unless otherwise noted, is existing normal experiment method in the prior art, detection method etc..
Embodiment 1：The separation of bacterial strain, screening
(1) separation of bacterial strain
Matrimony vine tree root border pedotheque 5 healthy, dying, in heaven is gathered from Zhongning County Shi Laba villages of Ningxia Province.Weigh
10.0 grams of matrimony vine Rhizosphere Soils, add the ratio of 90mL sterilized waters to be put into triangle of the preprepared equipped with bead in every 10 grams of soil
In bottle, 180rpm concussions 20-30min on 37 DEG C of constant-temperature tables is placed in.Gradient dilution method, 10 are diluted to by soil supension-2、10-3、10-4、10-5、10-6Etc. different gradients.The soil liquid 0.1mL for drawing appropriate dilution is placed in LB plating mediums, is coated with
Uniformly (this experiment chooses 10-4、10-5、10-6Three gradients are coated).37 DEG C of constant incubator culture 24h, picking single bacterium
Fall.The bacterial strain of acquisition is purified with the method for three rides, until obtaining single bacterium colony, and bacterial strain is numbered, protected
It is stored in 4 DEG C of refrigerators standby.
(2) screening of Antagonistic Fungi
Primary election is carried out to Antagonistic Fungi using flat board face-off method.The bacterial strain activation that will be preserved, it is standby.To be stored on inclined-plane
Matrimony vine Pathogens Causing Root Rot Disease is activated in PDA plate using scribble method, 28 DEG C of cultures to covering with flat board, with the blue electron gun head of sterilizing or
Above-mentioned pathogen bacterium block, the middle part of PDA plate is transferred to tweezers under card punch vertical cutting, is placed in 28 DEG C of incubator cultures
One day.The Antagonistic Fungi activated with toothpick picking is inoculated at pathogen edge 2cm, is placed in 28 DEG C of incubators and is continued to cultivate 2-
5 days, the appearance of antibacterial band is seen whether, measure the width of antibacterial band.Bacteriostatic diameter is measured simultaneously, calculates bacteriostasis rate.
Bacteriostasis rate (%)=(colony diameter D- bacteriostatic diameters d)/colony diameter D × 100%.
2. result of the test：
The selection best bacterial strain of one plant of fungistatic effect, strain number is GQJK2, and its flat board dual test the results are shown in Table 1.
Table 1：Bacteriostasis rate measurement result
As can be seen from Table 1：Bacterial strain GQJK2 has preferable bacteriostasis to the pathogen of matrimony vine root rot.
2. the form of bacterial strain GQJK2 and physiological and biochemical property are determined
(1) colony characteristicses and thalli morphology
The bacterium colony and thalline of the bacterial strain are characterized as：Bacterium colony is in subcircular, milk yellow, bacterium colony after cultivating 24h on LB flat boards
Flat, the small dentation in edge, dry tack free, colony diameter is 2.57mm.Thalline is in elongated rod shape, produces gemma, Gram-positive.
(2) physiological and biochemical property
The physiological and biochemical property of the bacterial strain is：Oxidizing ferment experiment is negative, and catalase experiment is positive, and citrate is using experiment
The positive, nitrate is negative using experiment, and Starch Hydrolysis experiment is negative, and M-R experiments are negative, and V-P experiments are positive, glucose fermentation reality
The positive is tested, sucrose fermenting experiment is positive, and maltose fermenting experiment is positive, and arabinose fermenting experiment is positive, wood-sugar fermentation experiment
The positive, cellobiose fermenting experiment is negative, and lactose fermentation experiment is negative, and sorbitol fermentation experiment is positive, mannose ferment experiment
It is positive.
3. the 16S rDNA of bacterial strain GQJK2 are determined and analysis
The 16S rDNA gene orders of bacterial strain GQJK2, as shown in SEQ ID NO.1 in nucleotides sequence list.To be surveyed
Sequence alignment in 16SrDNA sequences and GenBank databases, as a result shows：GQJK2 and Bacillus tequilensis
(10b) is in together in a minimum branch, the bacterial strain of its 16S rRNA gene order and Bacillus tequilensis (10b)
Sequence similarity is 99%, with reference to above-mentioned physiological and biochemical property result, identifies that GQJK2 is Te Jila bacillus (Bacillus
tequilensis)。
Bacterial strain GQJK2, Classification And Nomenclature be Te Jila bacillus (Bacillus tequilensis) GQJK2, in
On November 10th, 2016 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address
For：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), culture presevation number is CGMCC
NO.13253。
Embodiment 2：The preparation of bacterial strain GQJK2 biological prevention and control agents
Specific preparation method is as follows：
Te Jila bacillus (Bacillus tequilensis) GQJK2 for the antagonism matrimony vine root rot that inclined-plane is preserved
It is transferred in the test tube equipped with 5mL bean sprout juice fluid nutrient mediums, is put in 37 DEG C, cultivates 12h in 180rpm constant-temperature tables.Then press
1% inoculum concentration is inoculated into the liquid bean sprouts medium of 100mL/250mL, is put in 37 DEG C, is trained in 180rpm constant-temperature tables
24h is supported, it is 3 × 10 to be made concentration8The bacteria suspension of cfu/mL.
Liquid bean sprouts medium, its raw material constitutes and is：Moyashi 100g, sucrose 50g, water 1000mL, pH nature；
Preparation method is：Moyashi boiling water boiling 30min, with four layers of filtered through gauze, adds sucrose constant volume to 1000mL.
Embodiment 3：Field test
1. test method：
The serious plot of selection matrimony vine root rot morbidity, designs two treatment, respectively processes 1：GQJK2 bacterium solutions (are implemented
It is prepared by example 2), every matrimony vine tree consumption is 25mL；Treatment 2：Compared with the clear water of equivalent.
Each treatment sets 3 repetitions, and each repeats to select 20 matrimony vine trees.
Specific test method：Pouring root is carried out to matrimony vine tree after bacterium solution is diluted into 200 times with clear water.The clear water of control equivalent
Pouring root.
2. result of the test：
(1) prevention effects of the bacterial strain GQJK2 to matrimony vine root rot
Result is as shown in table 2.
Table 2：Matrimony vine tree morbidity statistics in field experiment
As can be seen from Table 2：
1. the incidence of disease：In field experiment, the incidence of disease of control is 90%, with Te Jila bacillus (Bacillus
Tequilensis) the matrimony vine tree incidence of disease of GQJK2 bacterium solutions treatment is 35%, compared with the control, the matrimony vine tree processed with GQJK2
The incidence of disease reduce 55 percentage points.
2. relative control effect：Phases of Te Jila bacillus (Bacillus tequilensis) GQJK2 to matrimony vine root rot
To preventive effect up to 75%.
(2) effect of increasing production of the bacterial strain GQJK2 to matrimony vine tree
The results are shown in Table 3.
Table 3：Effect of increasing production of Te Jila bacillus (Bacillus tequilensis) GQJK2 to matrimony vine tree
As can be seen from Table 3：The matrimony vine tree average product of control treatment is 0.5kg/, uses Te Jila bacillus
The yield of (Bacillus tequilensis) GQJK2 bacterium solutions treatment is 3.25kg/, compared with the control, with GQJK2 treatment
The averagely volume increase 550% of matrimony vine tree.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
It is any to be familiar with those skilled in the art in the technical scope of present disclosure, technology according to the present invention scheme and its invention
Design is subject to equivalent or change, should all be included within the scope of the present invention.
SEQUENCE LISTING
<110>Shandong Agricultural University
<120>The Te Jila bacillus of one plant of antagonism matrimony vine root rot and its application
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1421
<212> DNA
<213>Bacterial strain GQJK2
<400> 1
ttcggcggct ggctcctaaa aggttacctc accgacttcg ggtgttacaa actctcgtgg 60
tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcatgc tgatccgcga 120
ttactagcga ttccagcttc acgcagtcga gttgcagact gcgatccgaa ctgagaacag 180
atttgtggga ttggcttaac ctcgcggttt cgctgccctt tgttctgtcc attgtagcac 240
gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc ttcctccggt 300
ttgtcaccgg cagtcacctt agagtgccca actgaatgct ggcaactaag atcaagggtt 360
gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca accatgcacc 420
acctgtcact ctgcccccga aggggacgtc ctatctctag gattgtcaga ggatgtcaag 480
acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 540
cccccgtcaa ttcctttgag tttcagtctt gcgaccgtac tccccaggcg gagtgcttaa 600
tgcgttagct gcagcactaa ggggcggaaa ccccctaaca cttagcactc atcgtttacg 660
gcgtggacta ccagggtatc taatcctgtt cgctccccac gctttcgctc ctcagcgtca 720
gttacagacc agagagtcgc cttcgccact ggtgttcctc cacatctcta cgcatttcac 780
cgctacacgt ggaattccac tctcctcttc tgcactcaag ttccccagtt tccaatgacc 840
ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc gagcccttta 900
cgcccaataa ttccggacaa cgcttgccac ctacgtatta ccgcggctgc tggcacgtag 960
ttagccgtgg ctttctggtt aggtaccgtc aaggtaccgc cctattcgaa cggtacttgt 1020
tcttccctaa caacagagct ttacgatccg aaaaccttca tcactcacgc ggcgttgctc 1080
cgtcagactt tcgtccattg cggaagattc cctactgctg cctcccgtag gagtctgggc 1140
cgtgtctcag tcccagtgtg gccgatcacc ctctcaggtc ggctacgcat cgttgccttg 1200
gtgagccatt acctcaccaa ctagctaatg cgccgcgggt ccatctgtaa gtggtagccg 1260
aagccacctt ttatgtttga accatgcggt tcaaacaacc atccggtatt agccccggtt 1320
tcccggagtt atcccagtct tacaggcagg ttacccacgt gttactcacc cgtccgccgc 1380
taacatcagg gagcaagctc ccatctgtcc gctcgactgc a 1421
Claims (10)
1. one plant of Te Jila bacillus of antagonism matrimony vine root rot, its Classification And Nomenclature is Te Jila bacillus (Bacillus
Tequilensis) GQJK2, has been preserved in China Committee for Culture Collection of Microorganisms commonly micro- on November 10th, 2016
(abbreviation CGMCC, address is Bio-Centers：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute), culture presevation number is CGMCC NO.13253.
2. a kind of biological prevention and control agent, its active component is the Te Jila bacillus (Bacillus described in claim 1
Tequilensis) the tunning of GQJK2.
3. biological prevention and control agent as claimed in claim 2, it is characterised in that the biological prevention and control agent is liquid bacterial agent.
4. biological prevention and control agent as claimed in claim 3, it is characterised in that Te Jila bacillus in the biological prevention and control agent
The content of (Bacillus tequilensis) GQJK2 is more than or equal to 3 × 108cfu/mL。
5. the preparation method of the biological prevention and control agent described in any one of claim 2-4, it is characterised in that comprise the following steps：Fermentation
Te Jila bacillus (Bacillus tequilensis) GQJK2 described in claim 1, obtains tunning.
6. preparation method as claimed in claim 5, it is characterised in that the culture medium that fermentation is used is liquid bean sprout juice culture
Base, its raw material constitutes and is：Moyashi 100g, sucrose 50g, water 1000mL, pH nature.
7. preparation method as claimed in claim 5, it is characterised in that the condition of fermentation is：35-40 DEG C, constant-temperature table culture
20-30h；Preferably, 37 DEG C, constant-temperature table culture 24h.
8. Te Jila bacillus (Bacillus tequilensis) GQJK2 described in claim 1 and/or claim 2
Application of the described biological prevention and control agent in matrimony vine root rot is prevented and treated.
9. Te Jila bacillus (Bacillus tequilensis) GQJK2 described in claim 1 and/or claim 2
Application of the described biological prevention and control agent in matrimony vine volume increase is promoted.
10. one kind can prevent and treat matrimony vine root rot, while promoting the product of matrimony vine volume increase, its active component is described in claim 1
Te Jila bacillus (Bacillus tequilensis) GQJK2 or claim 2 described in biological prevention and control agent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568248A (en) * | 2017-10-10 | 2018-01-12 | 石河子大学 | A kind of controlling plant diseases simultaneously strengthen microbial bacterial agent of plant stress-resistance and its preparation method and application |
| CN110117554A (en) * | 2018-02-05 | 2019-08-13 | 呼伦贝尔恒屹农牧业股份有限公司 | A kind of microbial bacterial agent, preparation method and applications |
Citations (2)
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| CN104403962A (en) * | 2014-10-31 | 2015-03-11 | 中国农业科学院农产品加工研究所 | Bacillus tequilensis and its use in prevention and control of fusarium graminearum |
| KR20160129369A (en) * | 2015-04-30 | 2016-11-09 | 한국생명공학연구원 | Composition for preventing plant storage disease and a control method of plant storage disease using the same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403962A (en) * | 2014-10-31 | 2015-03-11 | 中国农业科学院农产品加工研究所 | Bacillus tequilensis and its use in prevention and control of fusarium graminearum |
| KR20160129369A (en) * | 2015-04-30 | 2016-11-09 | 한국생명공학연구원 | Composition for preventing plant storage disease and a control method of plant storage disease using the same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568248A (en) * | 2017-10-10 | 2018-01-12 | 石河子大学 | A kind of controlling plant diseases simultaneously strengthen microbial bacterial agent of plant stress-resistance and its preparation method and application |
| CN110117554A (en) * | 2018-02-05 | 2019-08-13 | 呼伦贝尔恒屹农牧业股份有限公司 | A kind of microbial bacterial agent, preparation method and applications |
| CN110117554B (en) * | 2018-02-05 | 2023-03-07 | 呼伦贝尔恒屹农牧业股份有限公司 | Microbial agent, preparation method and application thereof |
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