CN111374915A - Anti-aging composition and preparation method thereof - Google Patents

Abstract

The invention discloses an anti-aging composition and a preparation method thereof, wherein the anti-aging composition comprises the following raw materials in parts by weight: 5-10 parts of aloe, 5-8 parts of black wolfberry, 10-20 parts of cactus, 0.5-2 parts of hydrolyzed sodium hyaluronate, 5-8 parts of hexapeptide, 10-20 parts of snow lotus herb and 5-8 parts of honeysuckle. The anti-aging composition has good anti-aging effect and small irritation.

Description

Anti-aging composition and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to an anti-aging composition and a preparation method thereof.

Background

With the improvement of living standard, people have urgent requirements on aging prevention and treatment, life prolonging, life vitality improvement and life quality improvement. Currently, the medical definition of aging is: general weakening of various functions of the organism, and phenomena or processes of resistance to environmental damage and reduced ability to restore homeostasis. The skin is the most easily exposed organ in aging process, and aging of skin is manifested by head and appearance, hair like eyebrow and hair becoming white, wrinkle of eyelid, rough skin, dryness, telangiectasia, etc. Skin aging is a complex biological phenomenon and mainly comprises two forms of natural aging and photoaging, wherein the photoaging is skin photoaging caused by long-term exposure of skin to ultraviolet radiation, the photoaging of skin is caused by increase of free radicals in the skin, the free radicals can damage biological membranes, promote crosslinking and polymerization of elastic fibers and accelerate skin aging, and the skin aging is determined to be the result of combined action of endogenous factors and exogenous factors.

At present, anti-aging cosmetics on the market are various in variety, and vitamin antioxidants (such as vitamin E and vitamin C) are mostly added, but the stability of the antioxidants is poor, and the application of the antioxidants is limited. In recent years, researchers find that some plants or Chinese herbal medicines contain antioxidant ingredients and have the effects of resisting free radicals, promoting skin metabolism and delaying body aging, but in practical application, the anti-wrinkle and anti-aging effects of the existing cosmetics containing the extracts of the plants or Chinese herbal medicines are not obvious.

Disclosure of Invention

In order to solve the problem that the anti-wrinkle and anti-aging effects of the existing cosmetics containing plant or Chinese herbal medicine extracts are not obvious, the invention aims to provide an anti-aging composition, and therefore, the invention also provides a preparation method of the anti-aging composition; in addition, the invention also provides cosmetics adopting the anti-aging composition.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides an anti-aging composition, which comprises the following raw materials in parts by weight: 5-10 parts of aloe, 5-8 parts of black wolfberry, 10-20 parts of cactus, 0.5-2 parts of hydrolyzed sodium hyaluronate, 5-8 parts of hexapeptide, 10-20 parts of snow lotus herb and 5-8 parts of honeysuckle.

Aloe is a perennial evergreen herb of Liliaceae, and has clustered leaves, large and thick leaves, a shape of a seat or growing on the top of a stem, everacicular leaves or short and wide leaves, and sharp dentations at the edges. The mucus in aloe is polysaccharide as core component, and the mucus is important component for preventing cell aging and treating chronic allergy. Mucin exists in human muscle and gastrointestinal mucosa, and makes the tissue elastic, and if insufficient mucin is available, the muscle and mucosa lose elasticity and become rigid and aging.

Lycium ruthenicum Murr of Solanaceae, Lycium genus, with multiple branches; the branches are obliquely lifted or horizontally laid on the ground, are white or grey white, hard, are usually zigzag and zigzag, have irregular longitudinal stripes, are gradually tapered at the top ends of the small branches to form a thorn shape, are shortened between the sections, are spherical and have thin skins, are purple-black after the skins are ripe, contain rich purplish-red pigment in fruits, are very easy to dissolve in water, and belong to natural water-soluble anthocyanin flavonoids. The Tibetan medicine is used for treating heart-heat diseases and heart diseases, reducing cholesterol, exciting cerebral nerves, enhancing immunity, preventing and treating cancers, resisting aging, maintaining beauty and keeping young, irregular menstruation, menopause and the like, and has obvious drug effect.

Cactus, a plant of the genus cactus. The cactus is bush with the upper branch being wide, inverted oval, elliptical or nearly circular; spoked, the receptacle is inverted oval; most seeds are oblate, slightly irregular in edge, hairless and light yellow brown. The cactus contains 8 kinds of amino acids and multiple trace elements essential to human body, and precious components such as the wallflower, the pedicel immortal and the jade lotus, and has the effects of clearing away heat and toxic materials, invigorating the stomach and tonifying the spleen, clearing heat from the throat and moistening the lung, beautifying and protecting the skin and the like on the human body.

The top of snow lotus herb is like lotus flower, so it is called snow lotus herb, which is called snow lotus herb for short. Is perennial herb and is 15-35 cm high. The snow lotus herb can accelerate the metabolism of the skin, reduce wrinkles, keep the skin glossy and plump and delay aging.

Honeysuckle flower, also known as honeysuckle flower. The honeysuckle has the effects of clearing away heat and toxic materials, resisting inflammation, tonifying deficiency and treating wind, and can harmonize the medicines.

The composite material comprises the following raw materials in parts by weight: 8 parts of aloe, 6 parts of black wolfberry, 10 parts of cactus, 1 part of hydrolyzed sodium hyaluronate, 5 parts of hexapeptide, 15 parts of snow lotus herb and 6 parts of honeysuckle.

The paint comprises the following components in parts by weight: 10 parts of aloe, 8 parts of black wolfberry, 15 parts of cactus, 0.5 part of hydrolyzed sodium hyaluronate, 6 parts of hexapeptide, 10 parts of snow lotus herb and 5 parts of honeysuckle.

In a second aspect of the present invention, there is provided a method for preparing the above antiaging composition, comprising the steps of:

s1, soaking aloe, lycium ruthenicum murr, cactus, snow lotus herb and honeysuckle in 20-80% ethanol according to the ratio of 1:5-1:10 of medicine to solvent for 2 hours, then stirring and extracting at 60 ℃ for 1-2 hours, repeatedly extracting for 2-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure and recovering ethanol until no alcohol smell exists, thus obtaining the traditional Chinese medicine extract;

s2, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract to obtain the anti-aging composition.

In a third aspect of the present invention, there is provided an antiaging cosmetic composition comprising the above antiaging composition.

Wherein the anti-aging cosmetic also comprises vegetable oil, water and an emulsifier.

Compared with the prior art, the invention has the following beneficial effects: the anti-aging composition prepared by the invention is prepared by compounding seven raw materials of aloe, lycium ruthenicum, cactus, hydrolyzed sodium hyaluronate, hexapeptide, snow lotus herb and honeysuckle, and has three main active ingredients: one is a component containing antioxidant factors, including snow lotus herb, honeysuckle and hexapeptide; the second component contains ultraviolet absorption factors, including aloe, black matrimony vine and cactus; and the third is a component containing water replenishing factor, such as hydrolyzed sodium hyaluronate. The three groups of components produce obvious synergistic effect. The components consisting of the Chinese globeflower, the honeysuckle and the hexapeptide contain antioxidant factors, eliminate redundant free radicals in vivo and reduce internal cell damage; the components consisting of aloe, black medlar and cactus effectively absorb ultraviolet rays through ultraviolet absorption factors and reduce external cell damage; moisturizing component composed of hydrolyzed sodium hyaluronate can improve skin elasticity. After the three components are compounded, the characteristics of the components are complementary, the anti-aging performance of the composition is far better than the effect of the three components when the three components are used independently, the skin permeability is strong, and the stability is good.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.

Example 1

A method for preparing an anti-aging composition, comprising the steps of:

s1, sequentially weighing 50g of aloe, 60g of black wolfberry, 150g of cactus, 5g of hydrolyzed sodium hyaluronate, 60g of hexapeptide, 120g of snow lotus herb and 60g of honeysuckle;

s2, sequentially adding aloe, lycium ruthenicum mill, cactus, snow lotus herb and honeysuckle in S1 into 20% ethanol water solution by volume fraction, wherein the amount of the ethanol water solution is 5kg, the soaking time is 2 hours, then stirring and extracting at 60 ℃ for 1.5 hours, repeatedly extracting for 3 times, combining extracting solutions, filtering, concentrating the filtrate under reduced pressure, and recovering ethanol until no ethanol smell exists, so as to obtain a traditional Chinese medicine extract;

s3, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract in S2 in sequence to obtain the anti-aging composition.

Example 2

A method for preparing an anti-aging composition, comprising the steps of:

s1, weighing 80g of aloe, 80g of black wolfberry, 200g of cactus, 15g of hydrolyzed sodium hyaluronate, 50g of hexapeptide, 100g of snow lotus herb and 50g of honeysuckle in sequence;

s2, sequentially adding aloe, lycium ruthenicum mill, cactus, snow lotus herb and honeysuckle in S1 into 20% ethanol water solution by volume fraction, wherein the amount of the ethanol water solution is 5kg, the soaking time is 2 hours, then stirring and extracting at 60 ℃ for 1.5 hours, repeatedly extracting for 3 times, combining extracting solutions, filtering, concentrating the filtrate under reduced pressure, and recovering ethanol until no ethanol smell exists, so as to obtain a traditional Chinese medicine extract;

s3, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract in S2 in sequence to obtain the anti-aging composition.

Example 3

A method for preparing an anti-aging composition, comprising the steps of:

s1, sequentially weighing 100g of aloe, 50g of black wolfberry, 100g of cactus, 20g of hydrolyzed sodium hyaluronate, 80g of hexapeptide, 200g of snow lotus herb and 80g of honeysuckle;

s2, sequentially adding aloe, lycium ruthenicum mill, cactus, snow lotus herb and honeysuckle in S1 into 20% ethanol water solution by volume fraction, wherein the amount of the ethanol water solution is 5kg, the soaking time is 2 hours, then stirring and extracting at 60 ℃ for 1.5 hours, repeatedly extracting for 3 times, combining extracting solutions, filtering, concentrating the filtrate under reduced pressure, and recovering ethanol until no ethanol smell exists, so as to obtain a traditional Chinese medicine extract;

s3, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract in S2 in sequence to obtain the anti-aging composition.

Example 4

An anti-aging skin cream containing the anti-aging composition of example 3 was formulated as follows

Comparative example 1

A method for preparing an anti-aging composition, comprising the steps of:

s1, sequentially weighing 20g of hydrolyzed sodium hyaluronate, 80g of hexapeptide, 200g of snow lotus herb and 80g of honeysuckle;

s2, sequentially adding the snowdrop flowers and the honeysuckle flowers in the step S1 into 20% of ethanol aqueous solution by volume, wherein the amount of the ethanol aqueous solution is 5kg, the soaking time is 2 hours, then stirring and extracting at 60 ℃ for 1.5 hours, repeatedly extracting for 3 times, combining extracting solutions, filtering, and concentrating the filtrate under reduced pressure to recover ethanol until no ethanol smell exists, so as to obtain the traditional Chinese medicine extract;

s3, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract in S2 in sequence to obtain the anti-aging composition. Comparative example 2

A method for preparing an anti-aging composition, comprising the steps of:

s1, weighing 100g of aloe, 50g of black wolfberry, 100g of cactus and 20g of hydrolyzed sodium hyaluronate in sequence;

s2, sequentially adding the aloe, the lycium ruthenicum murr and the cactus in the S1 into 20% ethanol water solution by volume, wherein the amount of the ethanol water solution is 5kg, the soaking time is 2 hours, then stirring and extracting the mixture at 60 ℃ for 1.5 hours, repeatedly extracting the mixture for 3 times, combining the extracting solutions, filtering the extracting solutions, and concentrating the filtrate under reduced pressure to recover ethanol until no ethanol smell exists, so as to obtain a traditional Chinese medicine extract;

s3, adding hydrolyzed sodium hyaluronate into the Chinese medicinal extract in the S2 in sequence to obtain the anti-aging composition.

First, free radical scavenging test

The following experiments were conducted by preparing 0.5% strength sample solutions of the antiaging compositions of examples 1 to 3 and comparative examples 1 and 2, respectively, as test samples.

1. Evaluation of superoxide anion radical scavenging ability

4.5mL of Tris-HCl buffer (0.05 mol/L, pH 8.2) was preheated in a water bath at 25 ℃ for 20 min. Then adding 1mL of sample and 0.4mL of 25mmol/L pyrogallol solution, mixing uniformly, reacting in a water bath at 25 ℃ for 5min, and adding 1mL of 8mol/L HCl aqueous solution to terminate the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. Blank control 1mL of sample in solvent was used instead of sample. The clearance (D) was calculated according to equation (1).

Superoxide anion radical clearance (%) [1- (A2/A1) ] × 100% (1)

Wherein A1 is the absorbance value of the blank control; a2 is the absorbance value of the sample.

2. Evaluation of hydroxyl radical scavenging ability

Sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube₄3mL，1mmol/L H₂O₂3mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, adding into a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; respectively adding 1mL of samples, shaking up, continuing heating in water bath for 15min, taking out and measuring the absorbance value, and calculating the hydroxyl radical scavenging rate of the samples according to the formula (2).

Hydroxyl radical clearance (%) ([ a1-a2- (a1-A3) ]/a1 × 100% (2)

Wherein A1 is the absorbance value of the reaction system before adding the sample; a2 is the absorbance value of the system after the hydroxyl radical is removed from the sample; a3 is the absorbance value of the system after hydroxyl radical scavenging by the blank control.

3. Results of the experiment

The results of the experiment are shown in table 1.

TABLE 1

	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2
						For superoxide anion removal%	92.5％	93.1％	91.7％	47％	35％
Hydroxy radical scavenging rate/%)	94.5％	96.2％	93.7％	42％	39％

As can be seen from table 1, the anti-aging compositions prepared in examples 1, 2 and 3 have a superoxide anion scavenging rate and a hydroxyl radical scavenging rate of more than 90%, and when the components aloe, lycium ruthenicum and cactus containing ultraviolet absorption factors are removed, the superoxide anion scavenging rate and the hydroxyl radical scavenging rate are 47% and 42%, respectively; when the components of snow lotus herb, honeysuckle and hexapeptide containing antioxidant factors are removed, the clearance rate of superoxide anion and the clearance rate of hydroxyl radical are respectively 35 percent and 39 percent. The aloe-lycium ruthenicum mill is compounded by seven components of aloe, lycium ruthenicum mill, cactus, snow lotus herb, honeysuckle, hexakistropeptide and hydrolyzed sodium hyaluronate, and an obvious synergistic effect is embodied.

Second, tyrosinase inhibition assay

1. Experimental Material

L-tyrosine solution (0.5mg/mL), tyrosinase (100U/mL), compound anti-aging skin care composition.

2. Instrument for measuring the position of a moving object

A 10mL test tube, a water bath, a vibrator and an ultraviolet spectrophotometer.

3. Test method

The anti-aging composition obtained in example 1 was added with deionized water to prepare 0.05%, 0.1%, 0.15%, 0.2% aqueous solutions, and the tyrosinase inhibitory activity of each aqueous solution was determined according to literature methods. The document is: ningqing and Xiaoyan and the like in 2008 in 03 published 'spice and essence cosmetics' in 'microbial enzyme anti-aging efficacy research'.

4. Results of the experiment

The results of the determination that the anti-aging composition inhibits tyrosinase are shown in table 2.

TABLE 2

Anti-aging composition concentration/%)	Inhibition of tyrosinase%
		0.05％	83.1％
0.1％	85.3％
		0.15％	87.2％
0.2％	90.3％

As can be seen from Table 2, the anti-aging composition of the present invention has a strong inhibition rate against tyrosinase, and the inhibition rate against tyrosinase increases with the increase of the concentration of the anti-aging composition, and when the concentration of the anti-aging composition reaches 0.2%, the inhibition rate against tyrosinase is as high as 90%.

Human body trial evaluation test

1. Experimental methods

The skin cream prepared in example 4 was used for human trial evaluation. Selecting 40 female volunteers of 35-45 years old, wherein the using method comprises the following steps: the cream prepared in example 4 was applied to the left face and not to the right face once a day for a two-month experimental period. Filling out a trial evaluation table according to sensory evaluation (a volunteer evaluation scoring system is adopted, wherein the highest score is 3 to indicate significant effect, 2 to indicate effective effect, 1 to indicate micro effect and 0 to indicate ineffective effect).

2. Results of the experiment

The statistics of the human trial evaluation results are shown in table 3.

TABLE 3

Evaluation results	Significant efficiency/%)	Effective rate%	Micro efficiency/%)	Without efficiency/%)
					Skin cream prepared in example 4	5％	72％	15％	8％

As shown in table 3, when the skin cream prepared in example 4 was used by the subjects, the significant efficiency was 5%, the effective rate was 72%, and the total effective rate + effective rate was 5% + 72% + 77%.

Fourth, human skin irritation test

The test method comprises the following steps: a patch test method for detecting the safety of a human body adopts a 48h closed patch test. 50 healthy volunteers aged 18-24 years without allergic history were selected. After cleaning the subject's back, the plaque test device with the cream of example 4 applied to the selected area of the back with a non-irritating tape, and after application, was pressed gently with the fingers to apply evenly to the skin for 48 h. The examinee is instructed to keep the spot part dry within 48h, and violent exercise, scratching the spot part, long-time sunlight irradiation and the like are avoided. And removing the spot paste after 48h, marking, judging under sufficient light after 30min and the like, and performing revisit observation within 72h and 96h respectively.

Adverse reaction grading criteria for the patch test are shown in table 4.

TABLE 4

The skin adverse reaction results of the skin cream prepared in example 4 are shown in table 5.

TABLE 5

Extent of reaction	Grade of rating	Number of test subjects	In proportion of
				—	0	50	100％
±	1	0	0％
				+	2	0	0％
++	3	0	0％
				+++	4	0	0％

As shown in table 5, skin reactions were negative in all subjects, and the anti-aging composition of the present invention was relatively mild and less irritating.

The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (6)

1. The anti-aging composition is characterized by comprising the following raw materials in parts by weight: 5-10 parts of aloe, 5-8 parts of black wolfberry, 10-20 parts of cactus, 0.5-2 parts of hydrolyzed sodium hyaluronate, 5-8 parts of hexapeptide, 10-20 parts of snow lotus herb and 5-8 parts of honeysuckle.

2. The anti-aging composition according to claim 1, comprising the following raw materials in parts by weight: 8 parts of aloe, 6 parts of black wolfberry, 10 parts of cactus, 1 part of hydrolyzed sodium hyaluronate, 5 parts of hexapeptide, 15 parts of snow lotus herb and 6 parts of honeysuckle.

3. The anti-aging composition according to claim 1, comprising the following components in parts by weight: 10 parts of aloe, 8 parts of black wolfberry, 15 parts of cactus, 0.5 part of hydrolyzed sodium hyaluronate, 6 parts of hexapeptide, 10 parts of snow lotus herb and 5 parts of honeysuckle.

4. A method of preparing the antiaging composition according to any one of claims 1 to 3, characterized by comprising the steps of:

s1, soaking aloe, lycium ruthenicum murr, cactus, snow lotus herb and honeysuckle in 20-80% ethanol according to the ratio of 1:5-1:10 of medicine to solvent for 2 hours, then stirring and extracting at 60 ℃ for 1-2 hours, repeatedly extracting for 2-3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure and recovering ethanol until no alcohol smell exists, thus obtaining the traditional Chinese medicine extract;

s2, adding hydrolyzed sodium hyaluronate and hexapeptide into the Chinese medicinal extract to obtain the anti-aging composition.

5. An antiaging cosmetic characterized by comprising the antiaging composition according to any one of claims 1 to 3.

6. The anti-aging cosmetic composition of claim 5, further comprising vegetable oil, water and an emulsifier.