CN106420531A - Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application - Google Patents
Traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function and application Download PDFInfo
- Publication number
- CN106420531A CN106420531A CN201611040287.6A CN201611040287A CN106420531A CN 106420531 A CN106420531 A CN 106420531A CN 201611040287 A CN201611040287 A CN 201611040287A CN 106420531 A CN106420531 A CN 106420531A
- Authority
- CN
- China
- Prior art keywords
- preserving moisture
- extract
- skin
- whitening skin
- whitening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The invention discloses a traditional Chinese medicine composite extract containing nepeta angustifolia and having skin whitening and moisturizing function. The composite extract is prepared from, by weight, 10-30 parts of nepeta angustifolia10-40 parts of lycium ruthenicum murr, 10-30 parts of malus toringoides, 10-40 parts of highland barley and 10-30 parts of sealwort. The traditional Chinese medicine composite extract containing nepeta angustifolia is characterized by being capable of whitening and moisturizing skin and being natural and safe; meanwhile, inner components of the composite extract conduct synergistic interactions.
Description
Technical field
The invention belongs to fine chemistry industry article of everyday use technical field and in particular to be a kind of to there is whitening skin and preserving moisture containing hiding Herba Schizonepetae
The Chinese medicine composition extract of effect and application.
Background technology
The colour of skin refers to the skin that human skin top layer is reflected because of pigmentation such as melanin, hemn, phylloerythrin
Color.Wherein, melanin plays a decisive role.Originally melanic effect is the radiation injury in order to prevent ultraviolet to skin.
Once melanin secretion surplus in skin, arise that speckle deposits, thus produce a series of problems cosmetically.
Melanin Producing reason is led to have many kinds, the such as environmental problem such as ultraviolet waste gas, active oxygen and some heredity
Factor, its Producing reason is considerably complicated, and universal result of study shows that the process of B16 cell is as follows, with intracellular cheese ammonia
Acid, as substrate, generates DOPA quinone in the presence of tryrosinase, is further oxidized to after DOPA quinone becomes dopachrome
5,6- dihydroxy indoles and 5,6- dihydroxy -2- hydroxy acid, the polymerization of these products ultimately generates aggressiveness melanin.In cellular metabolism
Promotion layer by layer under be transferred to skin surface, if can not be metabolized in time excreting, will build up precipitation formed color
Speckle, ultimately results in hyperchromlsm.And have the reason lead to xerosis cutiss a lot, mainly skin water deficient, profit are secreted not
Balance and collagen protein run off etc. and to cause, and show as skin-bound, individual sites drying is itched, skin color dimness etc.
Deng, and xerosis cutiss are also unfavorable for further skin protection.
In studying at present, skin-whitening agents are all generally using tryrosinase as action target spot, by suppressing tryrosinase
Activity or blocking oxidizing tyrosine generates the melanic generation to reduce dermal melanin for the reaction, thus it is beautiful to reach skin
White effect.Great majority use hydrogen peroxide, ammoniated mercury and various phenol derivatives as skin-whitening agents, these
Compound can rapidly disintegrate the effect that melanin tissue reaches quick whitening, but have corrosivity, cytotoxicity to skin
With harmful effects such as anaphylaxis, safety is poor.Additionally, the whitening agent inhibiting mechanism of one-component relatively simple it is impossible to reach
Preferably whitening effect.For skin moisture-keeping, mostly rest on Physical moisturizing or the stage using moisture saver mask at present,
These products can provide certain moisture-keeping function, but moisture preserving time is shorter, and safety aspect can not be guaranteed, and to skin
Skin also has certain stimulation.
At present, whitening skin and preserving moisture product has many kinds on the market, but mostly all contains the huge sum of money such as lead, hydrargyrum in some whitening products
Belong to material.In the present invention, multiple natural extracts are applied in combination, both can meet the effect of whitening skin and preserving moisture, ensure that again
Skin not irriate, no sensation of pricking.
Hide the herb that Herba Schizonepetae hides Herba Schizonepetae Nepeta angustifolia C.Y.Wu for labiate.It originates in Tibet
(Jiangzi, Lhasa), is Tibet distinctive Tibetan medicine material.Hide Herba Schizonepetae be clinically mainly used in coma convulsion faint, apoplexy, epilepsy, brain overflow
Blood, skin ulcer injure the treatment of the diseases such as pain, have good curative effect.《Jingzhubencao》Point out：This product " controls epilepsy, cerebral hemorrhage, skin ulcer is hindered
And the disease such as pain ",《Tibetan medicine will》、《Medicine name is assembled》Also there are Tibetan Herba Schizonepetae treatment apoplexy, evil disease, pleuritis and dermopathic record.
Modern study finds, hides Herba Schizonepetae and can play the effect such as reducing swelling and alleviating pain, wound healing.Additionally, hiding some groups of Herba Schizonepetae volatile oil
Divide and there is antioxidation, antiinflammatory, analgesic effect.
Malus toringoides (Rehd.) Hughes leaf is also referred to as E Seye, is the medical material of Tibetan Autonomous Prefecture of Garze Tibetan medicine-food two-purpose among the people, derives from
The dried leaves of rosaceous plant Malus toringoides (Rehd.) Hughes Malus toringoides (Rehd.) Hughes.It is recorded in earliest《Tibetan medicine crystalline substance mirror
Book on Chinese herbal medicine》With《Hide Chinese voluminous dictionary》, record according to this two medical books, this medical instrument has assaulting fortified position to amass, except work(such as the stagnant, hepatic cholagogics of greasy paint
Effect, its magical medicinal efficacy has been logged the Tibetanmedicine works of most authority《Four doctor's allusion quotations》.Malus toringoides (Rehd.) Hughes leaf is made
With traditional Tibetan medicine's usage is oral with boiling with butter.It is mainly used in treating dyspepsia and some liver diseases, its determined curative effect,
Usage is unique.Modern pharmacological research shows, contains higher Flavonoids in Malus toringoides (Rehd.) Hughes leaf, its to DPPH free radical and
Hydroxyl radical free radical has preferable scavenging capacity, and in Malus toringoides (Rehd.) Hughes leaf, chemical composition phlorhizin, phloretin have anti-atherogenic
The pharmacological actions such as hardening, antiinflammatory.
Black Fructus Lycii leaf is the stem and leaf of Solanaceae Lycium black Fructus Lycii Lycium Ruthenicum Murr..At present,
The research of black Fructus Lycii focuses mostly in the extraction of pigment and polysaccharide contained by its fruit and biological activity aspect, and its blade is extracted
Thing has no report to beautifying whitening.Modern study finds, in black Fructus Lycii leaf, total flavones are to removing mice serum hydroxyl radical free radical
Impact, the protective effect to erythrocyte hemolysis and suppression mouse liver MDA generation aspect all there is remarkable efficacy, show
Significantly antioxidant activity.Research worker of the present invention finds that Lycium ruthenicum Murr. leaf extract has inhibitory action to tryrosinase, shows
Certain white-skinned face function.
Semen avenae nudae Hordeum vulgareLinn.var.nudum Hook.f. is hull-less barley, is grass family Hordeum crop,
Mainly it is distributed the Qinghai-Tibet Plateans such as Tibet, Qinghai, the Ganzi in Sichuan and Aba Prefecture, the enlightening celebrating in Yunnan, the Gannan in Gansu in China
Area, is exactly one of staple food crop of the Tibetan area people since ancient times.Semen avenae nudae is contained within great amount of soluble fiber, β-Portugal gathers
The nutritional labelings such as sugar, vitamin, mineral.Semen avenae nudae pigment content in Semen avenae nudae is higher.Modern study finds, Semen avenae nudae pigment has
Antioxidation, adjust blood fat, prevent the pharmacological actions such as coronary heart disease, antiinflammatory and antitumor it is also possible to wide as UV absorbent
General it is applied to cosmetic industry.Animal experiment study shows, Semen avenae nudae pigment is higher than black big for the scavenging capacity of hydroxyl radical free radical
Bean, Radix Ophiopogonis etc. other natural pigment；Mice serum antiactive oxygen ability can be strengthened, suppression peroxide MDA generates, notable fall
MDA content in low each organ-tissue.
Rhizoma Polygonati Polygonatum sibiricum is Liliaceae Polygonatum herbaceos perennial, and abnormal smells from the patient is gentle, and quality is grown
Profit, has the effect of the moon of tonic lung, moisturizes and promote the production of body fluid.Polygonatum polysaccharide is the principle active component of Rhizoma Polygonati water extract, is also Rhizoma Polygonati master
Want one of biological active ingredients.Modern pharmacology confirms, polygonatum polysaccharide has blood sugar lowering, blood fat reducing, strengthens immunologic function, antioxygen
Change, anti-ageing function of waiting for a long time.The removing of interior free yl depends on the activity of the antioxidases such as SOD, GSH-Px, and MDA is as body
The product of lipid peroxidation, can reflect that body cell is attacked, by free radical, the degree damaged.Animal experiment study shows,
Polygonatum polysaccharide can substantially reduce rat blood serum, skeletal muscle MDA content, improves the activity of rat blood serum endogenouss SOD and GSH-Px
And skeletal muscle endogenouss SOD activity, illustrate that polygonatum polysaccharide has stronger antioxidation, anti-aging effects.
Content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, provides a kind of Herba Schizonepetae containing Tibetan to have whitening skin and preserving moisture, natural peace
Entirely, the Chinese medicine composition extract of Synergistic and application.
In order to realize foregoing invention purpose, technical scheme is as follows：
A kind of have the Chinese medicine composition extract of whitening skin and preserving moisture effect containing hiding Herba Schizonepetae, this combined extracts by following by weight
The raw material of Chinese medicine of part meter is prepared from：Hide Herba Schizonepetae 10-30 part, black Fructus Lycii leaf 10-40 part, Malus toringoides (Rehd.) Hughes leaf 10-30 part, Semen avenae nudae
10-40 part, Rhizoma Polygonati 10-30 part.
Preferably, this Chinese medicinal compound extract is prepared from by following raw material of Chinese medicine according to the mass fraction：Hide Herba Schizonepetae 10-
20 parts, black Fructus Lycii leaf 20-40 part, Malus toringoides (Rehd.) Hughes leaf 10-20 part, Semen avenae nudae 20-40 part, Rhizoma Polygonati 10-20 part.
The described Herba Schizonepetae containing Tibetan has the preparation method of the Chinese medicine composition extract of whitening skin and preserving moisture effect, comprises the following steps：
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 5-8 times amount (V/m) 50%-80% ethanol, reflux, extract, 1-3 time, extracts 1-3 hour every time, filtration, and filtrate is closed
And, under the conditions of 55-75 DEG C decompression recycling ethanol to no alcohol taste, upper D101 type or AB-8 type macroporous resin column purification, collect
40%-70% ethanol elution part, decompression recycling ethanol under the conditions of 60 DEG C, obtain extractum, be vacuum dried under the conditions of 50-70 DEG C, powder
Broken, obtain final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 10-20 times amount water, unsaturated carbonate according to mass ratio
It is 8.0 that sodium solution adjusts pH value, heating and refluxing extraction 1-3 hour, and extracting solution 3000-5000rpm/min is centrifuged 15-30min, takes
Supernatant, adjusting pH value with the hydrochloric acid solution of 1mol/L is 6.0, adds Thermostable α-Amylase enzymolysis by Semen avenae nudae inventory 5u/g
Starch, 85-95 DEG C of enzymolysis 30-45 minute, checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature,
3000-5000rpm/min is centrifuged 15-30min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, places
Night.3000-5000rpm/min is centrifuged 15-30min, takes supernatant, and adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into
Solid-liquid ratio 1:3(m:V), plus 95% ethanol to alcohol content be 70%, stirring, stand overnight.Sucking filtration, obtains precipitation, is dried, as blue or green
Highland barley, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
The Chinese medicine composition extract that a kind of above-mentioned Herba Schizonepetae containing Tibetan has whitening skin and preserving moisture effect is preparing answering in skin care item
With.
Weight/mass percentage composition in whitening skin and preserving moisture skin care item for the described Chinese medicine composition extract with whitening skin and preserving moisture effect
For 0.1-10%, preferred mass percentage composition is 1-8%, and remaining is conventional substrate and the deionized water of cosmetics exterior-applied formulation
Or purified water or distilled water.By common process, can be made into whitening and moisturizing facial cleanser and preparation method of facial cleanser, whitening skin and preserving moisture Cleansing Foam, whitening skin and preserving moisture are washed
Facial cream, whitening skin and preserving moisture cream, whitening skin and preserving moisture frost, whitening skin and preserving moisture emulsion, whitening skin and preserving moisture water, whitening moisturizing face masque, whitening skin and preserving moisture gel
Or the cosmetics exterior-applied formulation such as whitening skin and preserving moisture soap.
The solution of the present invention is theoretical based on related medical tradition, in conjunction with modern medicine and pharmacology investigative technique and handss
Section, by many experiments preferably with checking it was found that hiding the five tastes such as Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf, Semen avenae nudae, Rhizoma Polygonati
Medical material uses cooperatively, and can play obvious whitening skin and preserving moisture skin care effect.Formula preferred for this invention, is examined by domestic and foreign literature
Rope, is not found related application, is screened by many experiments in addition, and gained formula proportion is optimum formula ratio, scientific formulation, and
Safe, can be used for daily skin nursing.
The present invention has advantages below：
(1) it is found that the whitening skin and preserving moisture that Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf, Semen avenae nudae, Rhizoma Polygonati use cooperatively is made
With expanding the range of application of above-mentioned raw materials, application particularly in the treatment of skin；
(2) by substantial amounts of experiment screening it is determined that the optimal proportion scope of raw material it is ensured that product of the present invention use effect
Really；
(3) inventive formulation definite effect and stable, it is unstable effectively to overcome natural product skin care item effect, also overcomes
The shortcomings of pure chemistry becomes the partial safety factor relatively low.
Specific embodiment
Further illustrate the technical solution of the present invention with reference to specific embodiment, these embodiments it is not intended that
It is the restriction to technical scheme.
Embodiment 1
Hide 10 parts of Herba Schizonepetae, 40 parts of black Fructus Lycii leaf, 10 parts of Malus toringoides (Rehd.) Hughes leaf, 30 parts of Semen avenae nudae, 10 parts of Rhizoma Polygonati
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 8 times amount (V/m) 70% ethanol, reflux, extract, 3 times, extracts 2 hours every time, filtration, and filtrate merges, in 65 DEG C of conditions
Lower decompression recycling ethanol is to no alcohol taste, upper AB-8 type macroporous resin column purification, collects 60% ethanol elution part, 60 DEG C of conditions
Lower decompression recycling ethanol, obtains extractum, is vacuum dried under the conditions of 70 DEG C, pulverizes, obtains final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 20 times amount water, saturated sodium carbonate according to mass ratio
It is 8.0 that solution adjusts pH value, heating and refluxing extraction 3 hours, and extracting solution 5000rpm/min is centrifuged 30min, takes supernatant, uses
It is 6.0 that the hydrochloric acid solution of 1mol/L adjusts pH value, adds Thermostable α-Amylase enzymatic starch by Semen avenae nudae inventory 5u/g, 95 DEG C
Enzymolysis 45 minutes, is checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature, 5000rpm/min is centrifuged
30min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.5000rpm/min is centrifuged 30min,
Take supernatant, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3(m:), plus 95% ethanol is to containing alcohol V
Measure as 70%, stir, stand overnight.Sucking filtration, obtains precipitation, is dried, as Semen avenae nudae, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
Embodiment 2
Hide 20 parts of Herba Schizonepetae, 20 parts of black Fructus Lycii leaf, 10 parts of Malus toringoides (Rehd.) Hughes leaf, 40 parts of Semen avenae nudae, 10 parts of Rhizoma Polygonati
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 6 times amount (V/m) 80% ethanol, reflux, extract, 2 times, extracts 3 hours every time, filtration, and filtrate merges, in 75 DEG C of conditions
Lower decompression recycling ethanol is to no alcohol taste, upper D101 type macroporous resin column purification, collects 70% ethanol elution part, 60 DEG C of conditions
Lower decompression recycling ethanol, obtains extractum, is vacuum dried under the conditions of 55 DEG C, pulverizes, obtains final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 15 times amount water, saturated sodium carbonate according to mass ratio
It is 8.0 that solution adjusts pH value, heating and refluxing extraction 2 hours, and extracting solution 3000rpm/min is centrifuged 20min, takes supernatant, uses
It is 6.0 that the hydrochloric acid solution of 1mol/L adjusts pH value, adds Thermostable α-Amylase enzymatic starch by Semen avenae nudae inventory 5u/g, 90 DEG C
Enzymolysis 30 minutes, is checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature, 3000rpm/min is centrifuged
20min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.3000rpm/min is centrifuged 20min,
Take supernatant, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3(m:), plus 95% ethanol is to containing alcohol V
Measure as 70%, stir, stand overnight.Sucking filtration, obtains precipitation, is dried, as Semen avenae nudae, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
Embodiment 3
Hide 15 parts of Herba Schizonepetae, 25 parts of black Fructus Lycii leaf, 10 parts of Malus toringoides (Rehd.) Hughes leaf, 35 parts of Semen avenae nudae, 15 parts of Rhizoma Polygonati
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 7 times amount (V/m) 80% ethanol, reflux, extract, 3 times, extracts 2 hours every time, filtration, and filtrate merges, in 55 DEG C of conditions
Lower decompression recycling ethanol is to no alcohol taste, upper AB8 type macroporous resin column purification, collects 50% ethanol elution part, 60 DEG C of conditions
Lower decompression recycling ethanol, obtains extractum, is vacuum dried under the conditions of 65 DEG C, pulverizes, obtains final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 10 times amount water, saturated sodium carbonate according to mass ratio
It is 8.0 that solution adjusts pH value, heating and refluxing extraction 3 hours, and extracting solution 4500rpm/min is centrifuged 15min, takes supernatant, uses
It is 6.0 that the hydrochloric acid solution of 1mol/L adjusts pH value, adds Thermostable α-Amylase enzymatic starch by Semen avenae nudae inventory 5u/g, 95 DEG C
Enzymolysis 45 minutes, is checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature, 4500rpm/min is centrifuged
30min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.4500rpm/min is centrifuged 30min,
Take supernatant, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3(m:), plus 95% ethanol is to containing alcohol V
Measure as 70%, stir, stand overnight.Sucking filtration, obtains precipitation, is dried, as Semen avenae nudae, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
Embodiment 4
Hide 10 parts of Herba Schizonepetae, 30 parts of black Fructus Lycii leaf, 20 parts of Malus toringoides (Rehd.) Hughes leaf, 20 parts of Semen avenae nudae, 20 parts of Rhizoma Polygonati
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 5 times amount (V/m) 80% ethanol, reflux, extract, 1 time, extracts 3 hours every time, filtration, and filtrate merges, in 60 DEG C of conditions
Lower decompression recycling ethanol is to no alcohol taste, upper D101 type macroporous resin column purification, collects 40% ethanol elution part, 60 DEG C of conditions
Lower decompression recycling ethanol, obtains extractum, is vacuum dried under the conditions of 50 DEG C, pulverizes, obtains final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 15 times amount water, saturated sodium carbonate according to mass ratio
It is 8.0 that solution adjusts pH value, heating and refluxing extraction 1 hour, and extracting solution 3500rpm/min is centrifuged 15min, takes supernatant, uses
It is 6.0 that the hydrochloric acid solution of 1mol/L adjusts pH value, adds Thermostable α-Amylase enzymatic starch by Semen avenae nudae inventory 5u/g, 85 DEG C
Enzymolysis 30 minutes, is checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature, 3500rpm/min is centrifuged
20min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.3500rpm/min is centrifuged 20min,
Take supernatant, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3(m:), plus 95% ethanol is to containing alcohol V
Measure as 70%, stir, stand overnight.Sucking filtration, obtains precipitation, is dried, as Semen avenae nudae, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
Embodiment 5
Hide 10 parts of Herba Schizonepetae, 25 parts of black Fructus Lycii leaf, 15 parts of Malus toringoides (Rehd.) Hughes leaf, 35 parts of Semen avenae nudae, 15 parts of Rhizoma Polygonati
(1) take dry Tibetan Herba Schizonepetae, black Fructus Lycii leaf, Malus toringoides (Rehd.) Hughes leaf medical material, pulverize, cross 20 mesh sieves, according to volume matter
Amount ratio adds 8 times amount (V/m) 55% ethanol, reflux, extract, 1 time, extracts 2 hours every time, filtration, and filtrate merges, in 65 DEG C of conditions
Lower decompression recycling ethanol is to no alcohol taste, upper AB-8 type macroporous resin column purification, collects 55% ethanol elution part, 60 DEG C of conditions
Lower decompression recycling ethanol, obtains extractum, is vacuum dried under the conditions of 60 DEG C, pulverizes, obtains final product above-mentioned three taste medicinal substances extracts.
(2) take dry Semen avenae nudae, Rhizoma Polygonati, pulverize, cross 40 mesh sieves, add 10 times amount water, saturated sodium carbonate according to mass ratio
It is 8.0 that solution adjusts pH value, heating and refluxing extraction 1.5 hours, and extracting solution 3000rpm/min is centrifuged 25min, takes supernatant, uses
It is 6.0 that the hydrochloric acid solution of 1mol/L adjusts pH value, adds Thermostable α-Amylase enzymatic starch by Semen avenae nudae inventory 5u/g, 90 DEG C
Enzymolysis 40 minutes, is checked to enzymolysis completely with iodine solution.Boil enzyme denaturing 10 minutes, let cool to room temperature, 4000rpm/min is centrifuged
25min, takes supernatant, and adjusting pH value with the hydrochloric acid solution of 1mol/L is 4.5, stands overnight.4000rpm/min is centrifuged 25min,
Take supernatant, adjusting pH value with saturated sodium carbonate solution is 7.0, is concentrated into solid-liquid ratio 1:3(m:), plus 95% ethanol is to containing alcohol V
Measure as 70%, stir, stand overnight.Sucking filtration, obtains precipitation, is dried, as Semen avenae nudae, Rhizoma Polygonati extract.
(3) mix homogeneously by the Semen avenae nudae obtaining, Rhizoma Polygonati extract with step (2) for the extract obtaining step (1), as this
The Herba Schizonepetae containing Tibetan of invention has the Chinese medicine composition extract of whitening skin and preserving moisture effect.
Embodiment 6
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract are tested to L-Tyrosine inhibition of enzyme activity
1 materials and methods：
1.1 sample
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract, arbutin (positive control).
1.2 experimental technique
(1) preparation of phosphate buffer：Precision weighs Na2HPO4 12H2O 26.797g, NaH2PO4 2H2O
13.803g, respectively after deionized water dissolving, is settled in 500mL volumetric flask.Take sodium dihydrogen phosphate and disodium phosphate soln
In each 100mL to 500mL volumetric flask, deionized water constant volume, shake up, as the phosphate buffer of pH=6.8.
(2) preparation of sample solution：Above-mentioned four kinds of sample DMSO are configured to concentration 0.1mg/mL, 0.2mg/ respectively
ML, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.7mg/mL, 0.8mg/mL testing sample solution.
(3) preparation of substrate L-Tyrosine solution：Substrate L-Tyrosine phosphate buffer is configured to mass concentration and is
0.05% L-Tyrosine solution.
(4) preparation of tyrosinase solution：Tryrosinase phosphate buffer solution is configured to the solution of 100U/mL.
(5) mensure of tyrosinase inhibition rate：Accurately draw tetra- groups of C1, C2, T1, T2 except tyrosinase solution by table 1
Reaction sample liquid, after 37 DEG C of constant temperature water baths 10 minutes, adds tyrosinase solution, reacts 10 minutes, the reference with C2 as C1,
System of measuring absorbance A 1 at 475nm, the reference with T2 as T1, measure system absorbance A 2.For what parallel sample was brought it is
System error, has been separately added into the DMSO of ImL in C1 and C2.For eliminating sample light absorbance error at 475nm, also add in T2
Enter lmL sample solution.Calculate the suppression ratio of tryrosinase by following equation, the results are shown in Table 2.
Wherein A1For the change of the absorbance of the reactant liquor of the product of not being loaded, A2For adding the absorbance of reactant liquor after sample
The change of value.
Table 1 tyrosinase inhibition test reagent dosage table
2 experimental results
The impact (%) to inhibitory activity against tyrosinase for table 2 extract of the present invention
Result shows, three kinds of embodiment extracts of the present invention all have good inhibiting effect to tyrosinase activity, wherein,
Preferably, in 0.2mg/mL, its suppression ratio reaches 50.06% to concentration to the inhibition of embodiment 1 extract, and embodiment 2 is extracted
In 0.4mg/mL, its suppression ratio is 58.27% to the concentration of thing, and in 0.4mg/mL, it suppresses the concentration of embodiment 3 extract
Rate is 52.14%, and three kinds of Example formulations also accordingly increase with the increase of concentration, the suppression ratio of tryrosinase, wherein, implements
The tyrosinase inhibition rate of example 1 is higher than totally embodiment 2 and embodiment 3.Result illustrates three kinds of embodiment extracts to tyrosine
Substantially, three kinds of embodiment extracts are respectively provided with good whitening effect to the inhibition of enzyme, wherein, especially with embodiment 1 extract
Best results, when concentration is for 0.8mg/mL, suitable with arbutin to the suppression ratio of tyrosinase activity.
Embodiment 7
Embodiment 1 extract, embodiment 2 extract, the experiment of embodiment 3 extract antioxidation
1 embodiment 1 extract, embodiment 2 extract, embodiment 3 extract remove the experiment of DPPH free radical
1.1 materials and methods
1.1.1 sample
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract, vitamin C (positive control).
1.1.2 experimental technique
By above-mentioned four kinds of sample dehydrated alcohol be configured to respectively concentration 0.05mg/mL, 0.06mg/mL, 0.07mg/mL,
0.08mg/mL, 0.09mg/mL, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL testing sample solution.
Take 2.5mL sample solution, the DPPH standard solution adding 2.5mL concentration to be 50mg/L, mix immediately, put into darkroom at room temperature
Reaction 30min, meanwhile, takes 2.5mL sample solution, adds 95% ethanol solution 2.5ml mix homogeneously, measure at 517nm
Absorbance.Calculate DPPH free radical scavenging activity by following equation, the results are shown in Table 3.
As：The absorbance of the mixed liquor of sample solution composition of 2.5mlLDPPH solution and 2.5mL variable concentrations；
A：The absorbance of the mixed liquor forming with 2.5mL dehydrated alcohol for the test fluid of 2.5mL variable concentrations；
A0：Absorbance for 2.5mL DPPH solution and the mixed solution of 2.5mL dehydrated alcohol composition.
1.2 experimental result
The impact (%) to DPPH free radical scavenging activity for table 3 extract of the present invention
2 embodiment 1 extract, embodiment 2 extract, embodiment 3 extract remove the experiment of ABTS free radical
2.1 materials and methods
2.1.1 sample
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract, vitamin C (positive control).
2.1.2 experimental technique
By above-mentioned four kinds of samples 95% ethanol be configured to respectively concentration 0.05mg/mL, 0.06mg/mL, 0.07mg/mL,
0.08mg/mL, 0.09mg/mL, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL testing sample solution.
Precision measures ABTS solution (being prepared by the distilled water) 0.20ml of 7mmol/L, molten with the potassium peroxydisulfate of the 2.6mmol/L of 0.20ml
Liquid mixes, and dilutes 40-50 times, makes the absorbance at solution 754nm be maintained at 0.7 ± 0.02, has both obtained the work of ABTS free radical
Liquid, keeps in Dark Place under room temperature.Draw 4mlABTS respectively to be sufficiently mixed uniformly with 1ml Tea Samples, stand 6min, with 95% second
Alcohol as blank photo, mensuration absorbance value at 754nm.Calculate clearance rate according to the following formula：
Wherein, A0For the ABTS free radical absorbance of itself, A is sample to the absorbance after ABTS Free Radical
Value.The results are shown in Table 4.
2.2 experimental result
The impact (%) to ABTS free radical scavenging activity for table 4 extract of the present invention
Above-mentioned antioxidation test result indicate that, three kinds of embodiment extracts of the present invention all have good antioxidation, its
In, the antioxidation of embodiment 1 extract is better than embodiment 2 and embodiment 3 extract.Modern study shows, melanin shape
Become relevant with oxygen-derived free radicals, therefore pass through scavenging activated oxygen, be effectively reduced melanic generation, play whitening function.Above-mentioned
Antioxidation experiment shows, the antioxidant activity of embodiment 1 extract is better than other embodiment, and result is pressed down with L-Tyrosine enzymatic activity
System experiment is consistent.
Embodiment 8
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract hygroscopicity and moisture retention measure
1 embodiment 1 extract, embodiment 2 extract, embodiment 3 extract hygroscopicity measure
1.1 materials and methods
1.1.1 sample
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract, Aloe powder (positive control).
1.1.2 experimental technique
Hydroscopicity measures：At room temperature, precision weighs above-mentioned drying to each 0.5000g of sample of constant weight, is respectively placed in training
In foster ware, culture dish is individually positioned in two exsiccators, in an exsiccator, is placed with saturated sodium carbonate solution (relative humidity
For 43%), be placed with saturated ammonium sulfate solution (relative humidity be 81%) in another exsiccator, each sample places 4,8,12,24,36,
48th, 60, weigh after 72h, be calculated as follows hydroscopicity：
The results are shown in Table 5 and table 6.
1.2 experimental result
Table 5 extract hygroscopicity of the present invention measures (%) (relative humidity is 43%)
Table 6 extract hygroscopicity of the present invention measures (%) (relative humidity is 81%)
Table 5 and table 6 result show, three embodiment extracts and Aloe powder under the conditions of relative humidity 81%, hydroscopicity
It is all higher than the hydroscopicity of relative humidity 43%.Three embodiment extracts all have good hydroscopicity, wherein, the suction of embodiment 1
Wet rate is substantially better than the extract of other two embodiments.
2 embodiment 1 extract, embodiment 2 extract, embodiment 3 extract moisturizing rate measure
2.1 materials and methods
2.1.1 sample
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract, Aloe powder (positive control).
2.1.2 experimental technique
Moisture retention measures：At room temperature, precision weighs above-mentioned drying to each 0.5000g of sample of constant weight, is respectively placed in training
In foster ware, addition mass fraction is 10% water, slowly rocks, enables sample to fully absorb moisture, is placed in equipped with dry silica gel
In exsiccator, each sample places 4,8,12,24,36,48,60, weigh after 72h, be calculated as follows hydroscopicity：
(H0Contain water quality, H for all product before placing1For placing sample size after a timing is fixed time).The results are shown in Table 7.
2.2 experimental result
Table 7 extract moisture retention of the present invention measures (%)
Table 7 result shows, three embodiment extracts of the present invention are consistent with hygroscopicity result with the moisture retention result of Aloe,
Three kinds of extracts all have good moisture retention, and in 24h, its moisturizing rate is all higher than 50%.Wherein, the guarantor of embodiment 1 extract
Wet effect is substantially better than other two embodiment extracts, and in 72h, its moisture retention remains above 50%.
Embodiment 9
The embodiment of the present invention 1 product, embodiment 2 product, embodiment 3 product normal individual trial evaluation experimental
The preparation of 1 whitening skin and preserving moisture frost
The present invention takes above-mentioned compound Chinese medicine extract to be configured to whitening skin and preserving moisture frost, and formula is following (by mass percentage)：
A phase：Stearic acid 3.0%
Vaseline 8.0%
Glyceryl monostearate 3.0%
Liquid Paraffin 10.0%
Hexadecanol 2.0%
Benzoic acid 0.2%
B phase：Purified water 55.0%
Propylene glycol 5.0%
Polyethylene glycol 1500 5.0%
Tween-40 3.0%
Triethanolamine 0.5%
Sodium sulfite 0.1%
C phase：Compound Chinese medicine extract 5.0%
D phase：Essence 0.2%
Preparation technology is as follows：
A phase is separately heated to 80 DEG C with B phase, is thoroughly mixed uniformly, under the conditions of 80 DEG C, B phase is stirred and adds A
Xiang Zhong, adds C phase, continues stirring, to temperature be down to 45 DEG C when, add D phase, stir to room temperature, obtain final product and have containing hiding Herba Schizonepetae
Whitening skin and preserving moisture frost.
Embodiment 1 extract, embodiment 2 extract, embodiment 3 extract are made corresponding whitening skin and preserving moisture according to the method described above
Frost, carries out corresponding human experimentation.
2 materials and methods
2.1 sample
Embodiment 1 extract whitening skin and preserving moisture frost, embodiment 2 extract whitening skin and preserving moisture frost, embodiment 3 extract whitening skin and preserving moisture
White, commercially available whitening skin and preserving moisture frost (main component：Radix Hamamelidis Molliss, Radix Ophiopogonis, Radix Sophorae Flavescentiss, Radix et Caulis Opuntiae Dillenii etc.).
2.2 experimental technique
Choose voluntary trier 80 people (male 40 people, women 40 people, age 18-25 year), face has pigementation, skin
The problems such as dark yellow, skin is dry and astringent, no cosmetic allergic contact dermatitis history.Volunteer is divided into 4 groups, every group of 20 people, M-F half and half.First
Group, embodiment 1 product on probation；Second group, embodiment 2 product on probation；3rd group, embodiment 3 product on probation, the 4th group, try out
Commercially available whitening skin and preserving moisture frost.
Using method：Sooner or later, after cleaning face, take appropriate Evaluation product uniform application in face, gently pat and absorb, and gently
Massage 3-5 minute, allows skin to fully absorb, and continuously tries out 8 weeks.By questionnaire form, investigate trier to used product
Subjective assessment, including whitening effect, the index such as moist, moisturizing and the dry and astringent, excitement of product.The results are shown in Table 8.
3 experimental results
Table 8 trier is to product evaluation application form
Table 8 is the evaluation table to Evaluation product for the trier, and the result in table shows, the embodiment of the present invention 1, embodiment 2 and
Embodiment 3 whitening skin and preserving moisture frost product has preferable whitening and moist moisture-keeping function, the particularly whitening function of embodiment 1 product
It is substantially better than commercially available prod, its effect is also superior to other two kinds of embodiment products.Three kinds of embodiment product all no allergy and stimulations
Sense, Product Safety is high.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment
Limit, other any spirit without departing from the present invention and the change made under principle, modification, replacement, combine, simplify,
All should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (6)
1. a kind of containing hide Herba Schizonepetae have whitening skin and preserving moisture effect Chinese medicine composition extract it is characterised in that this combined extracts by
Following raw material of Chinese medicine by weight are prepared from：Hide Herba Schizonepetae 10-30 part, black Fructus Lycii leaf 10-40 part, Malus toringoides (Rehd.) Hughes leaf
10-30 part, Semen avenae nudae 10-40 part, Rhizoma Polygonati 10-30 part.
2. a kind of Herba Schizonepetae containing Tibetan according to claim 1 has the Chinese medicine composition extract of whitening skin and preserving moisture effect, its feature
It is, this combined extracts is prepared from by following raw material of Chinese medicine by weight：Hide Herba Schizonepetae 10-20 part, black Fructus Lycii leaf
20-40 part, Malus toringoides (Rehd.) Hughes leaf 10-20 part, Semen avenae nudae 20-40 part, Rhizoma Polygonati 10-20 part.
3. the Chinese medicine composition extract that a kind of Herba Schizonepetae containing Tibetan according to claim 1 has whitening skin and preserving moisture effect is beautiful in preparation
Application in white Moisturizer.
4. according to claim 3 application it is characterised in that：The described Chinese medicinal compound extract with whitening skin and preserving moisture effect
Weight/mass percentage composition in whitening skin and preserving moisture skin care item is 0.1-10%.
5. according to claim 3 application it is characterised in that：The described Chinese medicinal compound extract with whitening skin and preserving moisture effect
Weight/mass percentage composition in whitening skin and preserving moisture skin care item is 1-8%.
6. according to claim 3 application it is characterised in that：Described whitening skin and preserving moisture skin care item be whitening and moisturizing facial cleanser and preparation method of facial cleanser,
Whitening skin and preserving moisture Cleansing Foam, whitening skin and preserving moisture wash one's face frost, whitening skin and preserving moisture cream, whitening skin and preserving moisture frost, whitening skin and preserving moisture emulsion, whitening skin and preserving moisture water,
The cosmetics exterior-applied formulations such as whitening moisturizing face masque, whitening skin and preserving moisture gel or whitening skin and preserving moisture soap.
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| CN107007526A (en) * | 2017-05-09 | 2017-08-04 | 上海绰然生物科技有限公司 | A kind of Preparation method and use of highland barley bran extract |
| CN108524392A (en) * | 2018-05-28 | 2018-09-14 | 上海应用技术大学 | A kind of sunscreen composition and suncream containing barley young leaf powder and calophyllum inophyllum kernel oil |
| CN111374915A (en) * | 2018-12-29 | 2020-07-07 | 上海洵都医疗科技有限公司 | Anti-aging composition and preparation method thereof |
| CN110227050A (en) * | 2019-07-26 | 2019-09-13 | 嘉兴言肌草生物技术有限公司 | A kind of anti-aging maintenance sustained release Essence and the anti-aging Face-protecting mask using its preparation |
| CN110227050B (en) * | 2019-07-26 | 2022-08-09 | 嘉兴言肌草生物技术有限公司 | Anti-aging repairing slow-release essence and anti-aging repairing mask prepared from same |
| WO2022218173A1 (en) * | 2021-04-16 | 2022-10-20 | 云南白药集团健康产品有限公司 | Facial mask liquid, and preparation method therefor and application thereof |
| WO2022218171A1 (en) * | 2021-04-16 | 2022-10-20 | 云南白药集团健康产品有限公司 | Application of pineapple extract composition in whitening |
| CN114010560A (en) * | 2021-11-18 | 2022-02-08 | 班戈卓攀健康产业有限责任公司 | Compound Tibetan herba schizonepetae composition with effects of brightening skin and resisting allergy and relieving and preparation method thereof |
| CN114010560B (en) * | 2021-11-18 | 2024-02-23 | 班戈卓攀健康产业有限责任公司 | Compound Tibetan herba schizonepetae composition with skin color brightening, anti-allergy and soothing effects and preparation method thereof |
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