CN111374088A - Artificial breeding method for giant salamander seedlings and special breeding pond thereof - Google Patents

Artificial breeding method for giant salamander seedlings and special breeding pond thereof Download PDF

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CN111374088A
CN111374088A CN201811653718.5A CN201811653718A CN111374088A CN 111374088 A CN111374088 A CN 111374088A CN 201811653718 A CN201811653718 A CN 201811653718A CN 111374088 A CN111374088 A CN 111374088A
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giant salamander
culture pond
artificial
water
parts
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唐自力
洪玉定
王煜恒
汤传全
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JURONG AQUATIC PRODUCT TECHNOLOGY DIRECTION STATION
Jiangsu Polytechnic College of Agriculture and Forestry
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JURONG AQUATIC PRODUCT TECHNOLOGY DIRECTION STATION
Jiangsu Polytechnic College of Agriculture and Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria

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  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Marine Sciences & Fisheries (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses an artificial giant salamander seedling culture method and a special culture pond thereof, and belongs to the technical field of aquaculture. The artificial breeding method of the giant salamander seedlings comprises the following steps: (1) sterilizing the culture pond and the water body; disinfecting the water body entering the culture pond by adopting ozone; (2) throwing the giant salamander seedlings for amplification; (3) feeding artificial baits; feeding with fresh yellow mealworms; (4) and (5) regularly patrolling. The invention adopts ozone and the extracting solution of the traditional Chinese medicine composition to disinfect the water body for the culture pond, has obvious effect, is environment-friendly and has no pollution. The invention provides the special culture pond using the culture method, the equipment is simple, and the investment cost is reduced.

Description

Artificial breeding method for giant salamander seedlings and special breeding pond thereof
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to an artificial breeding method for giant salamander seedlings and a special breeding pond thereof.
Background
Giant salamanders are the largest and most precious amphibians existing in the world. It sounds much like a baby's cry, so it is called a "giant salamander". The giant salamander is a national second-level protection amphibian wild animal, and is a major development in agricultural industrialization and special agriculture and a wild animal gene protection variety. The giant salamanders have very high values in view of appreciation, nutrition and medicine, so the market value of the giant salamanders is always high or low, the giant salamanders are continuously developed for the breeding and breeding industry of the giant salamanders, but the giant salamanders have very high requirements on living environment.
The artificial breeding technology of the giant salamanders is less affected by natural environment conditions, high in controllability and high in spawning rate, but the technical requirements of artificial breeding on operators are high, the prevention of plant diseases and insect pests of the giant salamanders also becomes a very critical part of the artificial breeding technology of the giant salamanders, and from the breeding environment and the giant salamanders, complete precautionary measures are needed to ensure that the artificial breeding of the giant salamanders has a high return rate; at present, the number of the senior giant salamanders engaged in artificial cognition in China is limited, and the market demand cannot be met.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the problems in the prior art, the invention aims to provide an artificial breeding method for giant salamander seedlings. The invention also aims to provide a special culture pond for the artificial culture method of the giant salamander seedlings. The invention is simple and easy to implement, has lower cost and obvious effect.
The technical scheme is as follows: in order to solve the problems, the technical scheme adopted by the invention is as follows:
an artificial breeding method of giant salamander seedlings comprises the following steps:
(1) sterilizing the culture pond and the water body: disinfecting the culture pond two months before stocking the giant salamander seedlings, and disinfecting the water body entering the culture pond by adopting ozone;
(2) and (3) throwing the giant salamander seedlings for amplification: the seedling should be strong, the muscle is fat, the body surface has no scar and parasite, and the cheek is complete and has no lesion before metamorphosis; after the selection is finished, gently placing the seedlings into a culture pond;
(3) feeding artificial baits: feeding artificial baits for 2-3 times a day at 8-10 pm per day; adding fresh yellow meal worms in the process of feeding artificial baits; the mass of the fresh and alive yellow meal worms is 20 to 40 percent of that of the artificial bait;
(4) and (3) regular patrol: during the inspection process, the water temperature change of the culture pond is observed, and the water temperature of the culture pond is controlled within the range of 18-23 ℃ all the time.
Preferably, the two times of disinfection of the culture pond are as follows: adopting quicklime dry powder for sterilization for the first time, and directly splashing the dry powder into a culture pond; standing for 5-7 days, and then injecting a small amount of water into the culture pond, wherein the depth of the water is 1/3 of the depth of the pond body; then carrying out secondary disinfection, and spraying the traditional Chinese medicine composition extracting solution into the culture pond; and (5) after the alkalinity in the tank body disappears, finishing the disinfection.
Preferably, the traditional Chinese medicine composition comprises 10-20 parts of fleece-flower root, 10-20 parts of wild chrysanthemum flower, 10-20 parts of folium artemisiae argyi, 5-10 parts of rhizoma atractylodis, 10-20 parts of garlic, 5-10 parts of astragalus membranaceus, 5-10 parts of saururus chinensis, 5-10 parts of cortex fraxini, 5-10 parts of galium aparine, 10-20 parts of coptis chinensis, 10-20 parts of sanguinea chinensis and 10-20 parts of purslane.
Preferably, the traditional Chinese medicine extracting solution is obtained by crushing the traditional Chinese medicine components until the particle size is 10-50 mm, boiling the traditional Chinese medicine components in water for 1-1.5 hours, and filtering the traditional Chinese medicine components.
Preferably, the concentration of the ozone is 1.0-1.5 mg/L, and the disinfection time is 20-40 min.
Preferably, the artificial bait is a sashimi, a live shrimp or a live fish.
Preferably, in the giant salamander breeding process, the traditional Chinese medicine composition extracting solution is added into a breeding pond, and the giant salamander is subjected to medicated bath treatment for 1 time in 1-2 months; the mass of the traditional Chinese medicine composition extracting solution is 1/10 of the mass of the water in the pool.
Preferably, the artificial baits and the fresh and alive yellow meal worms are sterilized by 2-3% of salt solution for 10-15 min before being fed to the giant salamander culture pond.
The special culture pond for the artificial culture method of the giant salamander seedlings comprises a culture pond body and a heating boiler, wherein heat insulation layers are arranged on the side surface and the bottom of the culture pond body, a heating pipeline is arranged in the heat insulation layers, and the heating pipeline is communicated with the heating boiler through a water return pipeline and a water inlet pipeline;
an oxygenation pipe and a baffle plate are arranged in the culture pond body.
Preferably, there are two of the oxygenation tubes; a water inlet control valve and a water outlet control valve are arranged between the water inlet pipeline and the heating pipeline as well as between the water inlet pipeline and the heating boiler; and a water inlet control valve and a water outlet control valve are arranged between the water return pipeline and the heating pipeline and between the water return pipeline and the heating boiler.
Has the advantages that: compared with the prior art, the invention has the advantages that:
(1) the invention adopts ozone and the extract of the traditional Chinese medicine composition to disinfect the water body for cultivation, has obvious effect, is environment-friendly and has no pollution.
(2) According to the invention, the traditional Chinese medicine composition extracting solution is adopted to carry out medicated bath treatment on the young giant salamanders in the giant salamander breeding process, so that the disease resistance of the young giant salamanders is improved, the survival rate of the young giant salamanders is obviously improved, and the disease rate is reduced by 20-30%.
(3) The invention is simple and easy to implement. The cost is low and the effect is obvious.
(4) The invention designs and develops the special culture pond applying the culture method, the equipment is simple, and the investment cost is reduced.
Drawings
FIG. 1 is a schematic structural view of a culture pond;
the aquaculture pond comprises a aquaculture pond body 1, a heat preservation layer 2, a heating boiler 3, a water return pipeline 4, a constant flow pump 5, a heating pipeline 6, an oxygenation pipe 7, a blocking plate 8 and a water inlet pipeline 9.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
Example 1
The structure of the special culture pond for the artificial culture method of the giant salamander seedlings comprises a culture pond body 1 and a heating boiler 3, wherein heat insulation layers 2 are arranged on the side surface and the bottom surface of the culture pond body 1, a heating pipeline 6 is arranged in the heat insulation layers 2, and the heating pipeline 6 is connected with the heating boiler 3 through a water return pipeline 4 and a water outlet pipeline 9; an oxygenation pipe 7 and a baffle plate 8 are arranged inside the culture pond body 1.
The oxygenation pipes 7 are arranged on two sides of the culture pond body 1 respectively, and in the disinfection stage of the culture pond, the oxygenation pipes 7 are used for introducing ozone, and the oxygenation pipes 7 are used for introducing oxygen in the giant salamander culture process, so that sufficient oxygen is provided for the giant salamander, and the giant salamander growth environment is more comfortable.
The barrier plate 8 is for using when feeding food, puts bait in barrier plate one side when giant salamander bait is thrown something and fed, makes the giant salamander be located the opposite side, treats bait and throws something and feeds after finishing, opens the barrier plate, and the giant salamander begins the feed, guarantees can not influence the rest of giant salamander at the in-process of throwing something and feeding bait.
The heat preservation layer 2 and the heating pipeline 6 are used for heating the water body in the culture pond, and the constant flow pump 5 provides power for water circulation, so that the water temperature is always maintained in an environment suitable for the growth of the giant salamanders; a water inlet control valve and a water outlet control valve are arranged between the water inlet pipeline 9 and the heating pipeline 6 as well as between the water inlet pipeline and the heating boiler 3; and water inlet and outlet control valves (not shown in the attached drawing) are arranged between the water return pipeline 4 and the heating pipeline 6 as well as between the water return pipeline and the heating boiler 3. During giant salamander is bred hot water supply breed pond heating in the heating boiler, when needing to change quality of water in breeding the pond, water inlet pipe 9 and return water pipeline 4 and heating boiler 3 disconnection to with insert in breeding the pond, change the water body to breeding the pond and handle.
The heating boiler 3 adopts a solar heating mode, so that the heat demand in the giant salamander breeding process can be met, the hot water for personnel and equipment in a working area can also be met, and the giant salamander breeding device is very environment-friendly and economical.
Example 2
An artificial breeding method of giant salamander seedlings comprises the following steps of (1) disinfecting a breeding pond and a water body: the breeding pond is disinfected two months before the giant salamander seedlings are released, and the two-time disinfection of the breeding pond is as follows: adopting quicklime dry powder for sterilization for the first time, and directly splashing the dry powder into a culture pond; after standing for 5 days, injecting a small amount of water into the culture pond, wherein the depth of the water is 1/3 of the depth of the pond body; then carrying out secondary disinfection, and spraying the traditional Chinese medicine composition extracting solution into the culture pond; and (5) after the alkalinity in the tank body disappears, finishing the disinfection.
The traditional Chinese medicine composition comprises 10 parts of fleece-flower root, 10 parts of wild chrysanthemum flower, 10 parts of folium artemisiae argyi, 5 parts of rhizoma atractylodis, 20 parts of garlic, 10 parts of astragalus membranaceus, 10 parts of saururus chinensis, 5 parts of cortex fraxini, 5 parts of galium aparine, 20 parts of coptis chinensis, 10 parts of hematoxylin and 20 parts of purslane, and is prepared by crushing the traditional Chinese medicine components until the particle size is 10mm, boiling the traditional Chinese medicine components in water for 1 hour, and filtering the decoction to obtain an extracting solution.
And the water body entering the culture pond is disinfected by ozone; the concentration of ozone is 1.0mg/L, and the disinfection time is 40 min.
(2) And (3) throwing the giant salamander seedlings for amplification: the seedling should be strong, the muscle is fat, the body surface has no scar and parasite, and the cheek is complete and has no lesion before metamorphosis; and after the selection is finished, gently placing the seedlings into the culture pond.
(3) Feeding artificial baits: the feeding time of the artificial bait sashimi is 8 and 10 o' clock per night and 2 times per day, and the temperature of the culture pond is 18 ℃ when the artificial bait is fed; the sashimi are sterilized by 2 percent salt solution for 10min before being fed to the giant salamander culture pond. In the giant salamander breeding process, adding a traditional Chinese medicine composition extracting solution into a breeding pond, and carrying out medicated bath treatment on the giant salamander for 1 time in 1 month; the mass of the Chinese medicinal composition extract is 1/10 of the mass of the water in the pool.
(4) And (3) regular patrol: in the patrol process, the water temperature change of the culture pond is observed, the water temperature of the culture pond is controlled within the range of 18-23 ℃, the action is light in the patrol process, and the rest and the activity of the giant salamander cannot be disturbed; when the night is patrolled, strong light is not needed to be used for correlation.
Example 3
An artificial breeding method of giant salamander seedlings comprises the following steps of (1) disinfecting a breeding pond and a water body: disinfecting the culture pond two months before stocking the giant salamander seedlings, and disinfecting the water body entering the culture pond by adopting ozone; the concentration of ozone is 1.5mg/L, and the disinfection time is 20 min.
The two-time disinfection of the culture pond comprises the following steps: adopting quicklime dry powder for sterilization for the first time, and directly splashing the dry powder into a culture pond; after standing for 7 days, injecting a small amount of water into the culture pond, wherein the depth of the water is 1/3 of the depth of the pond body; then carrying out secondary disinfection, and spraying the traditional Chinese medicine composition extracting solution into the culture pond; and (5) after the alkalinity in the tank body disappears, finishing the disinfection.
The traditional Chinese medicine composition comprises 20 parts of fleece-flower root, 10 parts of wild chrysanthemum flower, 20 parts of folium artemisiae argyi, 10 parts of rhizoma atractylodis, 10 parts of garlic, 5 parts of astragalus membranaceus, 10 parts of saururus chinensis, 5 parts of cortex fraxini, 10 parts of hydrangea macrophylla, 10 parts of coptis chinensis, 20 parts of hematoxylin and 10 parts of purslane, and is prepared by crushing the traditional Chinese medicine composition components to a particle size of 30mm, boiling the mixture in water for 1.5 hours, and filtering the mixture to obtain an extracting solution.
(2) And (3) throwing the giant salamander seedlings for amplification: the seedling should be strong, the muscle is fat, the body surface has no scar and parasite, and the cheek is complete and has no lesion before metamorphosis; after the selection is finished, gently placing the seedlings into a culture pond;
(3) feeding artificial baits: the feeding time of the artificial bait live shrimps is 8, 9 and 10 points per night and 3 times per day; before feeding the giant salamanders into the giant salamander culture pond, 2% saline solution is adopted for disinfection treatment for 15min, and the temperature of the culture pond is ensured to be 23 ℃ when feeding live shrimps. In the giant salamander culture process, adding a traditional Chinese medicine composition extracting solution into a culture pond, and carrying out medicated bath treatment on the giant salamander for 1 month; the mass of the Chinese medicinal composition extract is 1/10 of the mass of the water in the pool.
(4) And (3) regular patrol: in the patrol process, the water temperature change of the culture pond is observed, the water temperature of the culture pond is controlled within the range of 18-23 ℃, the action is light in the patrol process, and the rest and the activity of the giant salamander cannot be disturbed; when the night is patrolled, strong light is not needed to be used for correlation.
Example 4
An artificial breeding method of giant salamander seedlings comprises the following steps of (1) disinfecting a breeding pond and a water body: disinfecting the culture pond two months before stocking the giant salamander seedlings, and disinfecting the water body entering the culture pond by adopting ozone; the concentration of ozone is 1.2mg/L, and the disinfection time is 25 min.
The two-time disinfection of the culture pond comprises the following steps: adopting quicklime dry powder for sterilization for the first time, and directly splashing the dry powder into a culture pond; standing for 6 days, and then injecting a small amount of water into the culture pond, wherein the depth of the water is 1/3 of the depth of the pond body; then carrying out secondary disinfection, and spraying the traditional Chinese medicine composition extracting solution into the culture pond; and (5) after the alkalinity in the tank body disappears, finishing the disinfection.
The traditional Chinese medicine composition comprises 12 parts of fleece-flower root, 15 parts of wild chrysanthemum flower, 18 parts of folium artemisiae argyi, 8 parts of rhizoma atractylodis, 15 parts of garlic, 7 parts of astragalus membranaceus, 6 parts of saururus chinensis, 9 parts of cortex fraxini, 7 parts of galium aparine, 14 parts of coptis chinensis, 13 parts of hematoxylin and 17 parts of purslane, and is prepared by crushing the traditional Chinese medicine composition into particles with the particle size of 50mm, boiling the particles in water for 1.5 hours, and filtering the boiled particles to obtain an extracting solution.
(2) And (3) throwing the giant salamander seedlings for amplification: the seedlings should be strong, have thick muscles and no scars and parasites on the body surface, and have complete and no lesion on the cheek before metamorphosis; after the selection is finished, gently placing the seedlings into a culture pond;
(3) feeding artificial baits: the feeding time of the artificial bait live fish is 8 and 10 points per night and 2 times per day; when feeding live fish, the temperature of the culture pond is ensured to be 20 ℃, and the live fish is disinfected for 13min by adopting 2.5 percent saline solution before being fed into the giant salamander culture pond. In the giant salamander culture process, adding a traditional Chinese medicine composition extracting solution into a culture pond, and carrying out medicated bath treatment on the giant salamander for 1 month; the mass of the traditional Chinese medicine composition extracting solution is 1/10 of the mass of the water in the pool.
(4) And (3) regular patrol: in the patrol process, the water temperature change of the culture pond is observed, the water temperature of the culture pond is controlled within the range of 18-23 ℃, the action is light in the patrol process, and the rest and the activity of the giant salamander cannot be disturbed; when the night is patrolled, strong light is not needed to be used for correlation.
Example 5
The conventional giant salamander seedling culture method comprises the following steps of (1) sterilizing a culture pond and a water body: disinfecting the culture pond two months before stocking the giant salamander seedlings, and only disinfecting by using quick lime dry powder;
(2) and (3) throwing the giant salamander seedlings for amplification: the seedling should be selected from strong physique, thick muscle, five scars on the body surface and parasites, and the cheek is complete and has no lesion before metamorphosis; after the selection is finished, gently placing the seedlings into a culture pond;
(3) feeding artificial baits: the feeding time of the artificial bait sashimi is 8 and 10 o' clock per night and 2 times per day;
(4) and (3) regular patrol: in the patrol process, the water temperature change of the culture pond is observed, the water temperature of the culture pond is controlled within the range of 18-23 ℃, the action is light in the patrol process, and the rest and the activity of the giant salamander cannot be disturbed; when the night is patrolled, strong light is not needed to be used for correlation.
The number of the giant salamander seedlings in the culture ponds is the same as that of the giant salamander seedlings in each culture pond, and 30 giant salamander seedlings are stocked in each culture pond. The three experimental groups are respectively the embodiments 2-4, the control group is the embodiment 5, after the giant salamanders are matured, the giant salamander yield of the experimental group is counted, and the result shows that the giant salamander yield of the experimental group is improved by about 20% compared with that of the control group, the disease incidence rate is reduced by about 30%, and the giant salamanders of the experimental group move more frequently. The data analysis shows that the daily gain of the giant salamanders in the experimental group and the control group is not greatly different, the yield is mainly improved, the death rate and the morbidity of the giant salamanders in the experimental group are obviously lower than those of the control group, and finally, the yield of the giant salamanders in the experimental group is improved by 20 percent compared with that of the control group.

Claims (10)

1. An artificial breeding method for giant salamander seedlings is characterized by comprising the following steps:
(1) sterilizing the culture pond and the water body: disinfecting the culture pond two months before stocking the giant salamander seedlings, and disinfecting the water body entering the culture pond by adopting ozone;
(2) and (3) throwing the giant salamander seedlings for amplification: the seedling should be strong, the muscle is fat, the body surface has no scar and parasite, and the cheek is complete and has no lesion before metamorphosis; after the selection is finished, gently placing the seedlings into a culture pond;
(3) feeding artificial baits: feeding artificial baits for 2-3 times a day at 8-10 pm per day; adding fresh yellow meal worms in the process of feeding artificial baits; the mass of the fresh and alive yellow meal worms is 20 to 40 percent of that of the artificial bait;
(4) and (3) regular patrol: during the inspection process, the water temperature change of the culture pond is observed, and the water temperature of the culture pond is controlled within the range of 18-23 ℃ all the time.
2. The artificial giant salamander seedling culture method according to claim 1, wherein the culture pond is disinfected twice as follows: adopting quicklime dry powder for sterilization for the first time, and directly splashing the dry powder into a culture pond; standing for 5-7 days, and then injecting a small amount of water into the culture pond, wherein the depth of the water is 1/3 of the depth of the pond body; then carrying out secondary disinfection, and spraying the traditional Chinese medicine composition extracting solution into the culture pond; the mass of the traditional Chinese medicine extracting solution is 1/20 of the mass of water in the pool body; and (5) after the alkalinity in the tank body disappears, finishing the disinfection.
3. The giant salamander seedling artificial breeding method according to claim 2, wherein the traditional Chinese medicine composition comprises, by weight, 10-20 parts of fleece-flower root, 10-20 parts of wild chrysanthemum flower, 10-20 parts of folium artemisiae argyi, 5-10 parts of rhizoma atractylodis, 10-20 parts of garlic, 5-10 parts of astragalus membranaceus, 5-10 parts of saururus chinensis, 5-10 parts of cortex fraxini, 5-10 parts of hydrangea macrophylla, 10-20 parts of coptis chinensis, 10-20 parts of sanguinaria japonica and 10-20 parts of purslane.
4. The giant salamander seedling artificial breeding method according to claim 3, wherein the traditional Chinese medicine composition extracting solution is obtained by crushing the traditional Chinese medicine components to a particle size of 10-50 mm, boiling in water for 1-1.5 h, and filtering.
5. The giant salamander seedling artificial breeding method according to claim 1, wherein the concentration of ozone is 1.0-1.5 mg/L, and the disinfection time is 20-40 min.
6. The giant salamander fry artificial breeding method according to claim 1, wherein the artificial bait is raw fish fillets, live shrimps or live fish.
7. The giant salamander seedling artificial breeding method according to claim 4, characterized in that in the giant salamander breeding process, the traditional Chinese medicine composition extracting solution is added into a breeding pond, and the giant salamander is subjected to medicated bath treatment for 1 time in 1-2 months; the mass of the traditional Chinese medicine composition extracting solution is 1/10 of the mass of the water in the pool.
8. The giant salamander seedling artificial breeding method according to claim 1, characterized in that the artificial baits and the fresh and live tenebrio molitor are sterilized by 2-3% of salt solution for 10-15 min before being fed to the giant salamander breeding pond.
9. The special culture pond for the artificial culture method of the giant salamander seedlings according to any one of claims 1 to 8, which is characterized by comprising a culture pond body (1) and a heating boiler (3), wherein heat-insulating layers (2) are arranged on the side surfaces and the bottom of the culture pond body (1), a heating pipeline (6) is arranged in each heat-insulating layer, and the heating pipeline (6) is communicated with the heating boiler (3) through a water return pipeline (4) and a water inlet pipeline (9);
an oxygenation pipe (7) and a baffle plate (8) are arranged in the culture pond body (1).
10. The special culture pond for the artificial culture method of the giant salamander seedlings according to claim 9, characterized in that the number of the oxygenation tubes (7) is two; a water inlet control valve and a water outlet control valve are arranged between the water inlet pipeline (9) and the heating pipeline (6) and between the water inlet pipeline and the heating boiler (3); and a water inlet control valve and a water outlet control valve are arranged between the water return pipeline (4) and the heating pipeline (6) and between the water return pipeline and the heating boiler (3).
CN201811653718.5A 2018-12-29 2018-12-29 Artificial breeding method for giant salamander seedlings and special breeding pond thereof Pending CN111374088A (en)

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