CN111296333A - Crossbreeding method for female high-body elegans and male walnuts - Google Patents
Crossbreeding method for female high-body elegans and male walnuts Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the field of aquaculture, in particular to a method for crossbreeding female elegans and male elegans vachelli. The method comprises the following specific processes: parent selection → isolated temporary rearing → artificial fertilization → debonding and hatching and fry rearing. The Ardisia vachelli is small in size and slow in growth speed; the high-body elegans is large and grows relatively fast. The invention realizes the crossbreeding of the female elegans sinensis and the male elegans vachelli by the technical processes of parent selection, isolated temporary culture, artificial fertilization, debonding incubation and fry culture. The method has the advantages that the female high-body elegans and the male walnuts can be hybridized, the new hybrid elegans variety with fast growth and good meat quality can be cultured, and the method has important significance for meeting the living needs of people, enriching the new fresh water culture variety and promoting the industrial development of elegans.
Description
Technical Field
The invention relates to the field of aquaculture, in particular to a method for crossbreeding female elegans and male elegans vachelli.
Background
The elegans jaborandi has tender meat, delicious taste and high economic value, belongs to one of 'three flowers and five fishes' in northeast, belongs to rare fresh water fishes and is deeply popular in the market. The Athyrea walekii (Leuciscus walekii Dybowski) is mainly distributed in various water systems of the drainage basin of Heilongjiang, downstream of the yellow river, \ 28390, river, Daihai, inner Mongolia Darli lake and the like. The Hippocampus japonicas (Leucisculus Linnaeus) is mainly distributed in Europe, northwest Asia, and is only found in the river system of Gorwis in China. The two kinds of Ardisia japonica belong to Cyprinus Carpio, Ardisia subfamily, and genus Ardisia; the high-body elegans belongs to typical cold water fishes, is large in size and can grow to 1kg generally; the walnuts' larch lives mainly in neutral fresh water environment, but can tolerate higher salinity and alkalinity, and the individual is relatively small, and the size is generally between 120 and 400 g. If two kinds of elegans can be interspecific hybridized, the filial generation shows a certain hybridization advantage in growth and meat quality, and is a new fresh water variety with great breeding potential.
At present, the breeding technology of the elegans has the following problems: (1) the breeding water temperature is low, the maturity of parent fish is poor, and the fertilization rate is low; (2) the water temperature for early culture of the seedlings is low, the biological bait in the pond is not easy to culture, and the survival rate of the seedlings after being put into the pond is low.
Disclosure of Invention
The invention aims to provide a method for crossbreeding female high-body deltoid and male gorgonian, which realizes the crossbreeding of the female high-body deltoid and the male gorgonian through the technical processes of parent selection, isolated temporary culture, artificial fertilization, debonding incubation and fry culture.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for crossbreeding female elegans and male elegans vachelli comprises the following steps:
(1) parent selection: a. female parent: selecting healthy high-body elegans of 3-5 ages with normal body, strong vitality and swollen abdomen; b. male parent: selecting 3-5-year-old Atalantic malay fish with normal body shape, strong vitality and obvious white granular 'chasing star' head;
(2) isolation and temporary culture: separating and temporarily culturing the male parent and the female parent in a pond by adopting a net cage or separating and temporarily culturing the male parent and the female parent in a cement pond; the temporary culture conditions are as follows: the water depth is 0.8-1.2 m, the water temperature is 8-12 ℃, the pH is 7.2-8.5, the dissolved oxygen is more than or equal to 5.0mg/L, and the temporary culture density is 10-30 tails/m2；
(3) Artificial hastening parturition: selecting female high-body elegans sinensis with a swollen and soft belly and an ovarian development stage IV to induce spawning, wherein the induced spawning water temperature is 8-12 ℃, and the induced spawning hormones and dosages used by the female fish are as follows: injecting 10-15 mu g of luteinizing hormone releasing hormone A into 1kg of fish body22000-3000 IU chorionic gonadotropin and 10-15 mg diutanone maleate; the male walnuts of Stewartia malabarica inject 5-8 mug luteinizing hormone releasing hormone A according to each 1kg body weight21000-1500 IU chorionic gonadotropin and 5-8 mg diutanone maleate; dissolving the oxytocic by 0.9 wt% of normal saline, uniformly mixing, injecting the oxytocic into parent bodies from back muscles or chest fin base parts at one time, wherein the injection depth is 5-10 mm, injecting male and female parents synchronously, and returning the parents to a temporary culture net cage or a cement pool for delivery after injecting hormone;
(4) artificial fertilization: injecting hormone for 40-50 h at the temperature of 8-12 ℃, extruding the abdomen of the parent, collecting ova and semen, adopting dry fertilization, mixing the ova of 3-5 female fishes and the semen of 1 male fish according to the proportion, stirring with feathers for 10-30 s, mixing uniformly, adding a 0.2-0.3 wt% sodium chloride aqueous solution with the volume 2-3 times that of the collected ova and semen, and continuously stirring for 5-10 s;
(5) and (3) debonding of fertilized eggs: adding 200-300 g of talcum powder into 10L of water to prepare talcum powder suspension; slowly pouring the fertilized eggs into the turbid liquid with the volume 4-6 times that of the fertilized eggs, and stirring while pouring; after all the fertilized eggs are poured, clockwise or anticlockwise stirring is carried out for 5-10 min, and then debonding is carried out; pouring the fertilized eggs and the talcum powder suspension into a handsheet sewn with a 40-mesh sieve, filtering to obtain the fertilized eggs, and rinsing the handsheet filled with the fertilized eggs in clear water for 3-5 times;
(6) hatching: adopting a two-step method for incubation, firstly putting fertilized eggs into an incubation barrel for incubation in micro-flow water, wherein incubation conditions are as follows: hatching density is 50-100 ten thousand grainsm3The dissolved oxygen is more than or equal to 5.0mg/L, and the incubation temperature is 10-14 ℃; when the eggs are incubated for 9 to 10 days, the embryo bodies can be seen to rotate in the egg shells by naked eyes, and the fertilized eggs in the incubation barrel are transferred to an incubation net cage or a cement pond which is sewn by a 40-mesh sieve for continuous incubation; controlling the water depth of the incubation net cage or the cement pond to be 0.8-1.2 m, laying fertilized eggs for 1.5-2 million grains per square meter, laying 1 air stone per 2 square meters, inflating continuously, incubating at the temperature of 9-11 ℃ for 1-2 days to obtain the primarily incubated larval fish;
(7) fry breeding: the newly hatched larval fish is cultivated in an incubation net cage or a cement pond under the following conditions: throwing 1.5-2 ten thousand fish fries per square meter, controlling the water temperature at 10-14 ℃, controlling the dissolved oxygen to be more than or equal to 5.0mg and controlling the pH value to be 7.2-8.4; feeding the larval fish for 7-9 days, wherein the larval fish is fed with particle feed with the particle size of 150-250 mu m and the crude protein content of not less than 50 wt% as an opening material for 2 times every day, and the feeding amount is calculated according to 3-6 g/ten thousand pieces; when the larval fish is 10-15 days old, continuously feeding the small particle feed with the particle size of 150-250 mu m and the crude protein content of not less than 50 wt%, wherein the feeding frequency is increased to 4 times per day, and the feeding amount is calculated according to 3-6 g/ten thousand times; and (5) culturing the larval fish after 15 days of age in the pond.
The method for crossbreeding the female high-body elegans and the male walnuts comprises the step (1), wherein the weight of the female high-body elegans is more than 250g, the weight of the male walnuts is more than 200g, and the number ratio of the female parents to the male parents is 2-3: 1.
The crossbreeding method of the female high-body elegans and the male elegans vachelli comprises the step (2), when the cement pond is temporarily cultured, the water change amount is not less than 1/4 of the total water volume every day, and no bait is thrown during the temporary culture.
In the step (3), in the female high-body aronia jalapa, the content of Follicle Stimulating Hormone (FSH) in serum is gradually increased along with the development of ovaries, and reaches the maximum value from the gonad development stage to the IV stage, which is obviously higher than that of other stages.
The method for crossbreeding the female elegans and the male elegans vachelli comprises the step (4), after injecting hormone for 40-50 hours, checking female and male parents every 1 hour.
The crossbreeding method of the female high-body elegans and the male walnuts comprises the following steps of (7) when a cement pond is adopted for cultivation: the larval fish is aged for 1-6 days, and the water amount is changed every day to 1/10 of the culture water body; changing the water amount of the larval fish to 1/5 of a cultivation water body every day when the larval fish is 7-9 days old; and changing water amount of the larval fish to 1/4 of the cultivation water body every day when the larval fish is 10-15 days old.
The invention has the following advantages and beneficial effects:
1. the invention adopts 1-time hormone injection, reduces workload and stimulation to parents under the condition of meeting the need of induced spawning, and reduces the death rate of the parents after induced spawning;
2. in the invention, when the dry fertilization is carried out, the preparation and use of the sodium chloride aqueous solution with the concentration of 0.2 to 0.3 weight percent can prolong the sperm movement time and is beneficial to improving the fertilization rate;
3. the invention adopts the commercialized corpuscle feed as the initial feed of the larval fish, reduce the dependence on active biological bait;
4. the larval fish is intensively cultivated in the net cage or the cement pond for a period of time, and the larval fish is delayed to leave the pond, so that the opening rate of the larval fish and the survival rate of the larval fish after leaving the pond are improved;
5. the invention is suitable for the industrialized production of hybrid elegans.
Drawings
FIG. 1 is a white granular "chasing star" diagram of the head of a male walnuts.
FIG. 2 is the selection chart of the parents (the left is the male fish of the Atalantic malabaricus, the female fish of the intermediate high-body Atalantic malabaricus, and the right is the ovary of the high-body Atalantic malabaricus).
Fig. 3 is a diagram of a parent temporary rearing cage.
Figure 4 is a graph of parental spawning induction.
FIG. 5 is a diagram of sperm and egg collection.
Fig. 6 is a hatching diagram of a microflow water hatching barrel.
FIG. 7 is a diagram of the membrane before rupture.
Fig. 8 is a diagram of an incubation net cage.
FIG. 9 is a diagram of the newly hatched fish (female Hi-somatic Atlantic elegans X male Atlantic elegans hance).
FIG. 10 is a diagram of 8-day-old swimming bladder inflated to start feeding compound feed.
FIG. 11 is a graph of a young fish of 15 days old.
Detailed Description
In the specific implementation process, the method for crossbreeding the female elegans sinensis and the male elegans vachelli sinensis comprises the following specific processes: parent selection → isolated temporary rearing → artificial fertilization → debonding and hatching and fry rearing. The Ardisia vachelli is small in size and slow in growth speed; the high-body elegans is large and grows relatively fast. The breeding technology of the hybrid elegans has important significance for enriching the breeding varieties of freshwater fishes in China and promoting the industrialized development of the elegans.
The present invention will be explained in further detail below by way of examples and figures.
Example 1
The method comprises the following steps:
(1) parent selection: and 3, 2019, 26 months and 8-12 ℃ of water. Selecting individuals with normal body types, no trauma, complete scales and strong vitality from the parents of 3 years old. As shown in figure 1, the male Stephania malabaricus selects 30 individuals with obvious white granular head chasing stars and weight of 200-320 g. 60 individuals with the weights of 251g to 410g and 60 tails of female high-body elegans are selected, wherein the abdomen of the female elegans is enlarged, and eggs in ovaries can be separated, and the female high-body elegans is shown in figure 2. In FIG. 2, the left part is the male Octopus vachelli, the middle female Atlantic, and the right part is the ovary of Atlantic.
(2) Isolation and temporary culture: as shown in figure 3, the selected male and female parents are respectively placed in a temporary culture net cage with the specification of 2.2m multiplied by 1.5m for isolated temporary culture, the temporary culture water depth is 1 m, the temporary culture water temperature is 8-12 ℃, the pH value is 7.2-8.5, the dissolved oxygen is more than or equal to 5.0mg/L, and the temporary culture density is 20 tails/m2。
(3) Artificial hastening parturition: and 3, 27 days after 3 months, selecting female high-body elegans with enlarged and soft abdomen and ovary development to the IV stage for induced spawning, wherein the induced spawning water temperature is 8-12 ℃, injecting induced spawning hormone into the female high-body elegans, and the hormone combination and the dosage are as follows: injection of 12. mu.g of luteinizing hormone releasing hormone A per kilogram of body mass2(LHRH-A2)+2500IU chorionic gonadotropin (HCG) +12mg diutanone maleate (DOM); the oxytocin is injected into the male fishes of the Atlantic malay, and the hormone combination and the dosage are as follows: 6 mug injection of luteinizing hormone releasing hormone A per kilogram of male fish physique of the walnuts2(LHRH-A2) +1500IU chorionic gonadotropin (HCG) +6mg diutanone maleate (DOM). After the oxytocin is dissolved by physiological saline with the concentration of 0.9 wt%, the oxytocin is injected to the basal part of the pectoral fin of the parent for 1 time, the depth of a needle is 5-10 mm, and the injected parent returns to a temporary culture net cage for delivery, as shown in figure 4.
(4) Artificial fertilization: and 3, 29 days after 3 months, slightly extruding the abdomen of the parent, collecting the ovum and the semen in a dry iron basin according to the proportion of 3-5 female fishes to 1 male fish, adopting dry fertilization, stirring with feathers for 10-20 s, uniformly mixing, pouring a 0.3 wt% sodium chloride aqueous solution (prepared by diluting 3 times of 0.9 wt% physiological saline) with the volume 3 times that of the collected ovum and semen, and continuously stirring for 5-10 s to form fertilized ovum, as shown in figure 5.
(5) And (3) debonding and hatching: adding 250g of talcum powder into 10L of water to prepare talcum powder suspension, pouring fertilized eggs into the talcum powder suspension with the volume 5 times that of the fertilized eggs, and stirring while pouring; after all the fertilized eggs are poured, stirring the fertilized eggs for 5min along the clockwise direction, filtering turbid talcum powder solution by using a 40-mesh handsheet, and rinsing the fertilized eggs in clear water for 3 times.
(6) Hatching: adopting a two-step incubation method, firstly placing fertilized eggs into an incubation barrel for incubation in micro-flow water (figure 6, the index of the micro-flow water is that the flow rate is that the fertilized eggs can be completely filled and scattered and overturned, the fertilized eggs can not overflow the incubation barrel, and the flow rate is generally controlled to be 2-4 kg/min), and the incubation condition is as follows: hatching density is 50 ten thousand grains/m3The dissolved oxygen is more than or equal to 5.0mg/L, and the incubation temperature is 10-14 ℃; when the eggs are incubated for 9 to 10 days, the embryo bodies can be seen to rotate in the egg shells by naked eyes (earlier stage of rupture of membranes, figure 7), at this time, the fertilized eggs in the incubation barrel are all transferred into an incubation net cage sewn by a 40-mesh sieve for continuous incubation (figure 8), at this time, the incubation temperature is 9 to 11 ℃, the water depth of the incubation net cage is controlled to be 0.8 to 1.2m, 1.5 to 2 ten thousand fertilized eggs are distributed per square meter, 1 air stone is placed per 2 square meters, and the air is filled continuously, so that the primarily incubated larvae can be obtained within 1 to 2 days (figure 9).
(7) Fry breeding: the newly hatched larval fish is cultivated in an incubation net cage under the following conditions: feeding 1.5-2 ten thousand fish fries per square meter, controlling the water temperature at 10-14 ℃, controlling the dissolved oxygen to be more than or equal to 5.0mg and controlling the pH value to be 7.2-8.4. 1-6 days old fries in the hatching net cage still have yolk sacs and are not eaten; 50% of larval fish aged 8 days are inflated with air, yolk sacs disappear, and the larval fish can swim smoothly and eat (figure 10), and micro-particle feed (with the particle size of 150-250 mu m) is fed for 2 times every day, wherein the feeding amount is calculated according to 3-6 g/ten thousand times; and when the larval fish is 10-15 days old, the feeding frequency is increased to 4 times per day, and the feeding amount is the same as the above. Larval fish after 15 days of age (fig. 11) can be grown in the pond.
The micro-particle feed of the embodiment comprises the following components: the red Sheng brand micro-particle compound feed produced by Shandong Sheng cable fish feed research center comprises the following main raw materials: the fish meal, the shrimp meal, the fish oil, the lecithin, the choline nitride, the vitamins and the vitamin-like substances, the monocalcium phosphate, the zinc sulfate, the ferrous sulfate and the like comprise the following main nutrients in percentage by mass: more than or equal to 50 percent of refined protein, more than or equal to 8 percent of crude fat, less than or equal to 3 percent of crude fiber, less than or equal to 16.5 percent of crude ash, less than or equal to 5 percent of calcium, less than or equal to 5 percent of total phosphorus, less than or equal to 12 percent of water and less than or equal to 2 percent of lysine.
Second, result in
Successfully induced spawning 52 tails of female elegans deltoidea with an induced spawning rate of 86.67 percent to obtain 32 thousand hybrid elegans eggs and 23.5 thousand tails of hybrid elegans deltoids with an incubation rate of 71.88 percent. The total length of the newly hatched fries is 7.06mm, the total length is 7.63mm when the fries begin to feed at the age of 8 days, and the fries grow to 8.62mm at the age of 15 days. The feeding ability of the 15-day-old fry is relatively strong to resist environmental changes, and the fry has higher survival rate when being put into a pond.
The embodiment result shows that the method of the invention can breed the new hybrid variety of the elegans which grows fast and has good meat quality by hybridizing the female elegans and the male elegans walskii, and has important significance for meeting the living needs of people and enriching the new variety of freshwater aquaculture.
Claims (6)
1. A crossbreeding method of female high-body elegans and male walnuts is characterized by comprising the following steps:
(1) parent selection: a. female parent: selecting healthy high-body elegans of 3-5 ages with normal body, strong vitality and swollen abdomen; b. male parent: selecting 3-5-year-old Atalantic malay fish with normal body shape, strong vitality and obvious white granular 'chasing star' head;
(2) isolation and temporary culture: separating and temporarily culturing the male parent and the female parent in a pond by adopting a net cage or separating and temporarily culturing the male parent and the female parent in a cement pond; the temporary culture conditions are as follows: the water depth is 0.8-1.2 m, the water temperature is 8-12 ℃, the pH is 7.2-8.5, the dissolved oxygen is more than or equal to 5.0mg/L, and the temporary culture density is 10-30 tails/m2；
(3) Artificial hastening parturition: selecting female high-body elegans sinensis with a swollen and soft belly and an ovarian development stage IV to induce spawning, wherein the induced spawning water temperature is 8-12 ℃, and the induced spawning hormones and dosages used by the female fish are as follows: injecting 10-15 mu g of luteinizing hormone releasing hormone A into 1kg of fish body22000-3000 IU chorionic gonadotropin and 10-15 mg diutanone maleate; the male walnuts of Stewartia malabarica inject 5-8 mug luteinizing hormone releasing hormone A according to each 1kg body weight21000-1500 IU chorionic gonadotropin and 5-8 mg diutanone maleate; dissolving the oxytocic by 0.9 wt% of normal saline, uniformly mixing, injecting the oxytocic into parent bodies from back muscles or chest fin base parts at one time, wherein the injection depth is 5-10 mm, injecting male and female parents synchronously, and returning the parents to a temporary culture net cage or a cement pool for delivery after injecting hormone;
(4) artificial fertilization: injecting hormone for 40-50 h at the temperature of 8-12 ℃, extruding the abdomen of the parent, collecting ova and semen, adopting dry fertilization, mixing the ova of 3-5 female fishes and the semen of 1 male fish according to the proportion, stirring with feathers for 10-30 s, mixing uniformly, adding a 0.2-0.3 wt% sodium chloride aqueous solution with the volume 2-3 times that of the collected ova and semen, and continuously stirring for 5-10 s;
(5) and (3) debonding of fertilized eggs: adding 200-300 g of talcum powder into 10L of water to prepare talcum powder suspension; slowly pouring the fertilized eggs into the turbid liquid with the volume 4-6 times that of the fertilized eggs, and stirring while pouring; after all the fertilized eggs are poured, clockwise or anticlockwise stirring is carried out for 5-10 min, and then debonding is carried out; pouring the fertilized eggs and the talcum powder suspension into a handsheet sewn with a 40-mesh sieve, filtering to obtain the fertilized eggs, and rinsing the handsheet filled with the fertilized eggs in clear water for 3-5 times;
(6) hatching: adopting a two-step method for incubation, firstly putting fertilized eggs into an incubation barrel for incubation in micro-flow water, wherein incubation conditions are as follows: the hatching density is 50-100 ten thousand grains/m3The dissolved oxygen is more than or equal to 5.0mg/L, and the incubation temperature is 10-14 ℃; when the eggs are incubated for 9 to 10 days, the embryo bodies can be seen to rotate in the egg shells by naked eyes, and the fertilized eggs in the incubation barrel are transferred to an incubation net cage or a cement pond which is sewn by a 40-mesh sieve for continuous incubation; controlling the water depth of the incubation net cage or the cement pond to be 0.8-1.2 m, laying fertilized eggs for 1.5-2 million grains per square meter, laying 1 air stone per 2 square meters, inflating continuously, incubating at the temperature of 9-11 ℃ for 1-2 days to obtain the primarily incubated larval fish;
(7) fry breeding: the newly hatched larval fish is cultivated in an incubation net cage or a cement pond under the following conditions: throwing 1.5-2 ten thousand fish fries per square meter, controlling the water temperature at 10-14 ℃, controlling the dissolved oxygen to be more than or equal to 5.0mg and controlling the pH value to be 7.2-8.4; feeding the larval fish for 7-9 days, wherein the larval fish is fed with particle feed with the particle size of 150-250 mu m and the crude protein content of not less than 50 wt% as an opening material for 2 times every day, and the feeding amount is calculated according to 3-6 g/ten thousand pieces; when the larval fish is 10-15 days old, continuously feeding the small particle feed with the particle size of 150-250 mu m and the crude protein content of not less than 50 wt%, wherein the feeding frequency is increased to 4 times per day, and the feeding amount is calculated according to 3-6 g/ten thousand times; and (5) culturing the larval fish after 15 days of age in the pond.
2. The method for crossbreeding the female high-body aronia sinensis and the male walnuts of the walnuts according to claim 1, wherein in the step (1), the weight of the female high-body aronia sinensis is more than 250g, the weight of the male walnuts is more than 200g, and the number ratio of the female parent to the male parent is 2-3: 1.
3. The method for crossbreeding female Hibiscus delbrueckii and male Hibiscus walbausii according to claim 1, wherein in step (2), the water change amount is not less than 1/4 of the total water volume per day when the cement pond is temporarily cultured, and no bait is thrown during the temporary culture period.
4. The method according to claim 1, wherein in the female Hibiscus delbrueckii, the content of Follicle Stimulating Hormone (FSH) in the serum gradually increases with the development of ovaries, and reaches a maximum value during gonad development to stage IV, which is significantly higher than that in each of the other stages, in the female Hibiscus delbrueckii.
5. The method for crossbreeding female and male Abelia sinensis according to claim 1, wherein in step (4), the female and male parents are checked every 1h after 40-50 h of hormone injection.
6. The method for crossbreeding female high-body elegans and male walnuts according to claim 1, wherein in the step (7), when the cement pond is adopted for cultivation: the larval fish is aged for 1-6 days, and the water amount is changed every day to 1/10 of the culture water body; changing the water amount of the larval fish to 1/5 of a cultivation water body every day when the larval fish is 7-9 days old; and changing water amount of the larval fish to 1/4 of the cultivation water body every day when the larval fish is 10-15 days old.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094925A (en) * | 2020-10-23 | 2020-12-18 | 中国水产科学研究院黑龙江水产研究所 | Method for rapidly identifying hybrid species of Hibiscus and Athletic crocus |
| CN113261517A (en) * | 2021-05-31 | 2021-08-17 | 中国水产科学研究院黑龙江水产研究所 | Bacteriostatic debonding agent, preparation method thereof and method for improving artificial breeding hatchability of Atalantic malabaricus via bacteriostatic debonding agent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
| CN107354226A (en) * | 2017-09-04 | 2017-11-17 | 中国水产科学研究院黑龙江水产研究所 | A kind of Leuciscus waleckii DNA bar code standard detection sequence and its application |
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
| CN209489314U (en) * | 2019-02-14 | 2019-10-15 | 吉林省水产科学研究院 | A kind of graining incubating oosperm device |
-
2020
- 2020-03-30 CN CN202010236608.XA patent/CN111296333A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
| CN107354226A (en) * | 2017-09-04 | 2017-11-17 | 中国水产科学研究院黑龙江水产研究所 | A kind of Leuciscus waleckii DNA bar code standard detection sequence and its application |
| CN209489314U (en) * | 2019-02-14 | 2019-10-15 | 吉林省水产科学研究院 | A kind of graining incubating oosperm device |
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
Non-Patent Citations (3)
| Title |
|---|
| 刘焕亮: "《水产养殖学概论》", 31 August 2000, 青岛出版社 * |
| 史为良: "《水产增养殖学研究》", 31 May 2009, 东北林业大学出版社 * |
| 张立民等: "四种常用鱼药对雅罗鱼杂交幼鱼的急性毒性", 《水产学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094925A (en) * | 2020-10-23 | 2020-12-18 | 中国水产科学研究院黑龙江水产研究所 | Method for rapidly identifying hybrid species of Hibiscus and Athletic crocus |
| CN113261517A (en) * | 2021-05-31 | 2021-08-17 | 中国水产科学研究院黑龙江水产研究所 | Bacteriostatic debonding agent, preparation method thereof and method for improving artificial breeding hatchability of Atalantic malabaricus via bacteriostatic debonding agent |
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