CN111279966A - Quercus mongolica rapid propagation method - Google Patents
Quercus mongolica rapid propagation method Download PDFInfo
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- CN111279966A CN111279966A CN202010220721.9A CN202010220721A CN111279966A CN 111279966 A CN111279966 A CN 111279966A CN 202010220721 A CN202010220721 A CN 202010220721A CN 111279966 A CN111279966 A CN 111279966A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for rapidly propagating quercus mongolica, and belongs to the technical field of agricultural cuttage. By adjusting the position of the branch for making the cutting slips, the components of the matrix, the nutrition condition, the growth hormone proportion, the humidity and the temperature, the survival rate of the quercus mongolica after cutting is improved to 90-95%, and the requirements of production and tests can be met. The invention has simple technology, is easy to master and is beneficial to popularization and use.
Description
Technical Field
The invention relates to the technical field of agricultural cuttage, in particular to a fast quercus mongolica propagation method.
Background
Quercus mongolica Fisch ex Ledeb belonging to Fagaceae, Quercus, deciduous arbor, up to 30 m, and dark longitudinal split. The crown is oval. Alternate single leaf, inverted oval shape of leaf, wavy tooth edge of leaf, dark green surface of leaf, light green back, parthenocarpy, hermaphrodite, oval or elliptical shape of nut, and fleshy cotyledon of seed. Is a second-grade precious tree species in China and is also a main secondary tree species in the northeast forest region of China. Mainly distributed in northeast, northwest and northwest China, and a small amount in China. Distribution is also found in russia, japan, mongolia and korean peninsula.
At present, research on quercus mongolica shows that callus can be generally formed in the cuttage process of quercus mongolica but cannot continuously take root and survive.
Therefore, how to rapidly promote the rooting and propagation of quercus mongolica is a problem which needs to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a fast quercus mongolica propagation method
In order to achieve the purpose, the invention adopts the following technical scheme:
a Quercus mongolicus rapid propagation method comprises the following steps:
(1) selecting the current year branches which grow robustly to prepare cutting slips after 5-6 months, wherein the cutting slips are 9-12cm long;
(2) preparing a cutting substrate;
(3) disinfecting the base part of the cutting prepared in the step (1), inserting mixed solution of IBA and 6-BA, soaking for 1.5-2.5h, inserting the cutting substrate prepared in the step (2), and keeping the humidity at 80-85% and the temperature at 25-30 ℃;
(4) and after the cuttage is finished, the insect damage is prevented, and after 15 days, the nursery stock is checked and does not survive and replanting.
Preferably: and (3) selecting the middle position of the branch to manufacture a cutting.
Preferably: the components of the cutting substrate in the step (2) are peat soil, vermiculite, perlite, coarse sand and a nutritional supplement, the volume ratio is 2: 1, wherein the coarse sand needs to be paved on the lowest layer.
Preferably: the raw materials of the nutritional supplement comprise, by weight, 200 parts of quercus mongolica leaves, 300 parts of straw, 1-3 parts of cellulomonas flavigena, 1-3 parts of rhodopseudomonas palustris and 1-3 parts of streptomyces, and the preparation method comprises the steps of crushing the quercus mongolica leaves and the straw, adding the cellulomonas flavigena, the rhodopseudomonas palustris and the streptomyces, atomizing, spraying water, adjusting the humidity to be 50-60%, and fermenting at normal temperature for 20-30 d.
In the preparation and fermentation process of the nutritional supplement, the nutritional supplement needs to be stirred every day, so that full fermentation is facilitated.
Preferably: in the mixed solution of IBA and 6-BA in the step (3), the concentration of IBA is 450mg/l, and the concentration of 6-BA is 2.2 mg/l.
According to the technical scheme, compared with the prior art, the invention discloses and provides the method for rapidly propagating the quercus mongolica, the technical effect is that the problem that only callus is formed but the quercus mongolica cannot root in the cuttage process is solved, and the survival rate reaches 90-95%.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a method for rapidly propagating quercus mongolica
The raw materials and medicines required by the invention are all commercial products without special requirements, for example, a single microbial strain can be purchased from North sodium biology company, and the source of the microbial strain is not limited. The non-emphasized method is a conventional method such as a sterilization method, a strain activation method, etc.
Example 1
A Quercus mongolicus rapid propagation method comprises the following steps:
(1) at the beginning of 5 months, selecting the current year branches which grow vigorously to prepare cutting slips, selecting the middle positions of the branches to prepare the cutting slips, wherein the length of the cutting slips is 9-12 cm;
(2) preparing a cutting substrate, wherein the cutting substrate comprises peat soil, vermiculite, perlite, coarse sand and a nutritional supplement in a volume ratio of 2: 1, wherein the coarse sand needs to be paved on the lowermost layer, and the nutritional supplement is prepared by fermenting non-diseased plants and a microbial agent;
(3) disinfecting the base part of the cutting prepared in the step (1), inserting a mixed solution consisting of IBA with the concentration of 450mg/l and 6-BA with the concentration of 2.2mg/l, soaking for 1.5h, inserting the cutting into the cutting substrate prepared in the step (2), and watering with enough water, wherein the humidity is 80 percent and the temperature is 25 ℃;
(4) after the cuttage is finished, insect damage is prevented and treated, after 15 days, the nursery stock is checked, and the survived reseeding is carried out
The raw materials of the nutritional supplement comprise 100g of quercus mongolica leaves, 200g of straws, 1g of cellulomonas flavigena, 1g of rhodopseudomonas palustris and 1g of streptomyces in parts by weight, and the preparation method comprises the steps of crushing the quercus mongolica leaves and the straws, adding the cellulomonas flavigena, the rhodopseudomonas palustris and the streptomyces, atomizing, spraying water, adjusting the humidity to 50%, and fermenting for 20d at normal temperature.
According to the method, 190 cuttings are produced in an agricultural planting base, 200 cuttings are produced for cuttage, and the cuttings are rooted and survive.
Example 2
The difference from example 1 is that the step (1) is 5 months end, the step (3) is soaked for 2.5h, the humidity is 85%, and the temperature is 30 ℃.
The raw materials of the nutritional supplement comprise 150g of quercus mongolica leaves, 250g of straws, 2g of cellulomonas flavigena, 2g of rhodopseudomonas palustris and 2g of streptomyces in parts by weight, and the preparation method comprises the steps of crushing the quercus mongolica leaves and the straws, adding the cellulomonas flavigena, the rhodopseudomonas palustris and the streptomyces, atomizing, spraying water, adjusting the humidity to 55%, and fermenting for 25d at normal temperature.
According to the method, 200 cuttings are produced in an agricultural planting base for cuttage, and the cuttings which root and survive reach 181 cuttings after 30 days of cuttage.
Example 3
The difference from example 1 is that step (1) is 6 months end, step (3) soaking time is 2h, humidity is 83%, and temperature is 27 ℃.
The raw materials of the nutritional supplement comprise 200g of quercus mongolica leaves, 300g of straws, 3g of cellulomonas flavigena, 3g of rhodopseudomonas palustris and 3g of streptomyces in parts by weight, and the preparation method comprises the steps of crushing the quercus mongolica leaves and the straws, adding the cellulomonas flavigena, the rhodopseudomonas palustris and the streptomyces, atomizing, spraying water, adjusting the humidity to 60 percent, and fermenting for 30 days at normal temperature.
According to the method, 200 cuttings are produced in an agricultural planting base for cuttage, and the cuttings which root and survive reach 184 cuttings 30 days after cuttage.
From examples 1-3, it can be seen that the cuttage survival rate of Quercus mongolicus rapidly propagated by the method reaches 85-95%.
Control test 1
Group 1, the difference from example 2 is that the position of the root of the shoot is selected for cuttage in the step (1).
Group 2, the difference from example 2, is that the matrix component of step (2) does not contain coarse sand.
Group 3, differing from example 2 in that no nutritional supplement was added in step (2).
Group 4, different from example 2 in that step (3) was a mixture of IBA at a concentration of 500mg/l and 6-BA at a concentration of 3 mg/l.
Group 5, differing from example 2 in that the humidity in step (3) was 95% and the temperature was 35 deg.C
200 cuttings are respectively cut in each group, and the statistics of survival rate and survival rate are shown in the following table 1
TABLE 1
| Group of | Survival rate (%) | Rooting percentage (%) | Root length (mm) |
| Group 1 | 43 | 46 | 8.8-14.2 |
| 2 groups of | 79 | 84 | 11-14.7 |
| Group 3 | 69 | 71 | 7.9-12.9 |
| 4 groups of | 89 | 93 | 10.2-14.8 |
| 5 groups of | 52 | 86 | 4.5-9.7 |
| Example 2 | 91 | 93 | 9.7-15.1 |
Data analysis shows that the survival rate of the 4 groups is basically consistent with that of the invention, but the dosage concentration is higher and is not necessary; 1, the low composition activity rate is related to the position of the branch for making the cutting, and the base part is not beneficial to survival; group 2 may have a certain influence on water retention because coarse sand is not spread at the bottom of the matrix; although the rooting rate of 5 groups is higher, the survival rate is low because the humidity or the temperature is not suitable for the cuttage survival of the Mongolian oak, and 3 groups show that the substrate is supplemented with nutrition, so that the rooting and survival of the cuttage can be promoted to a certain extent.
In the present specification, the embodiments are described in a progressive manner, each embodiment focuses on the differences from the other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. A Quercus mongolicus rapid propagation method is characterized by comprising the following steps:
(1) selecting the current year branches which grow robustly to prepare cutting slips after 5-6 months, wherein the cutting slips are 9-12cm long;
(2) preparing a cutting substrate;
(3) disinfecting the insertion stable base prepared in the step (1), inserting mixed solution of IBA and 6-BA, soaking for 1.5-2.5h, inserting the cutting substrate prepared in the step (2), and keeping the humidity at 80-85% and the temperature at 25-30 ℃;
(4) and after the cuttage is finished, the insect damage is prevented, and after 15 days, the nursery stock is checked and does not survive and replanting.
2. The method for rapidly propagating quercus mongolica as claimed in claim 1, wherein the branches of step (1) are selected from middle positions to make cuttings.
3. The method for rapidly propagating quercus mongolica as claimed in claim 1, wherein the cutting matrix component in the step (2) is peat soil, vermiculite, perlite, coarse sand and a nutritional supplement, and the volume ratio is 2: 1, wherein the coarse sand needs to be paved at the lowest layer.
4. The method for rapidly propagating Quercus mongolica according to claim 3, wherein the raw materials of the nutritional supplement comprise 200 parts by weight of Quercus mongolica leaves 100-200 parts by weight of straw 200-300 parts by weight of straw, 1-3 parts by weight of Cellulomonas flavigena, 1-3 parts by weight of Rhodopseudomonas palustris and 1-3 parts by weight of Streptomyces streptomyces, and the method comprises the steps of crushing the Quercus mongolica leaves and straw, adding Cellulomonas flavigena, Rhodopseudomonas palustris and Streptomyces streptomyces, adjusting the humidity by atomizing and spraying water to 50-60%, and fermenting at normal temperature for 20-30 d.
5. The method for rapidly propagating Quercus mongolica according to claim 1, wherein in the mixed solution of IBA and 6-BA in the step (3), the concentration of IBA is 450mg/l, and the concentration of 6-BA is 2.2 mg/l.
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| CN202010220721.9A CN111279966A (en) | 2020-03-24 | 2020-03-24 | Quercus mongolica rapid propagation method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715068A (en) * | 2012-07-16 | 2012-10-10 | 雷文东 | Water-retention soilless culture medium |
| CN103694039A (en) * | 2013-12-09 | 2014-04-02 | 武汉市林业果树科学研究所 | Lightweight culture medium of fine camellia rosthorniana and container seedling raising method |
| CN106105984A (en) * | 2016-07-01 | 2016-11-16 | 锦屏县源成农业科技开发有限公司 | A kind of Kadsura coccinea (Lem.) A.C. Smith cuttage raise seedling technique |
| CN108243817A (en) * | 2018-01-29 | 2018-07-06 | 辽宁省蚕业科学研究所 | A kind of Mongolian oak green branch cuttage breeding method |
| CN109169170A (en) * | 2018-10-12 | 2019-01-11 | 黑龙江省林业科学研究所 | Cutting medium used in Mongolian oak breeding method and this method |
| CN109362736A (en) * | 2018-12-07 | 2019-02-22 | 辽宁省林业科学研究院 | A kind of Mongolian oak cuttage root-growing agent and its application |
-
2020
- 2020-03-24 CN CN202010220721.9A patent/CN111279966A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715068A (en) * | 2012-07-16 | 2012-10-10 | 雷文东 | Water-retention soilless culture medium |
| CN103694039A (en) * | 2013-12-09 | 2014-04-02 | 武汉市林业果树科学研究所 | Lightweight culture medium of fine camellia rosthorniana and container seedling raising method |
| CN106105984A (en) * | 2016-07-01 | 2016-11-16 | 锦屏县源成农业科技开发有限公司 | A kind of Kadsura coccinea (Lem.) A.C. Smith cuttage raise seedling technique |
| CN108243817A (en) * | 2018-01-29 | 2018-07-06 | 辽宁省蚕业科学研究所 | A kind of Mongolian oak green branch cuttage breeding method |
| CN109169170A (en) * | 2018-10-12 | 2019-01-11 | 黑龙江省林业科学研究所 | Cutting medium used in Mongolian oak breeding method and this method |
| CN109362736A (en) * | 2018-12-07 | 2019-02-22 | 辽宁省林业科学研究院 | A kind of Mongolian oak cuttage root-growing agent and its application |
Non-Patent Citations (5)
| Title |
|---|
| 王昌杰等: "柞树扦插育苗 ", 《生命世界》 * |
| 金继华等: "蒙古栎扦插试验 ", 《吉林林业科技》 * |
| 闫文涛等: "不同生长调节剂对蒙古栎嫩枝扦插生根的影响", 《西北林学院学报》 * |
| 黄秦军等: "蒙古栎嫩枝扦插繁殖技术研究 ", 《西南林业大学学报》 * |
| 黄秦军等: "蒙古栎嫩枝扦插繁殖技术研究", 《西南林业大学学报》 * |
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Application publication date: 20200616 |