CN110974741A - 一种茶叶复提取物及应用 - Google Patents
一种茶叶复提取物及应用 Download PDFInfo
- Publication number
- CN110974741A CN110974741A CN201911202476.2A CN201911202476A CN110974741A CN 110974741 A CN110974741 A CN 110974741A CN 201911202476 A CN201911202476 A CN 201911202476A CN 110974741 A CN110974741 A CN 110974741A
- Authority
- CN
- China
- Prior art keywords
- tea
- extract
- leaves
- old
- mixing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000284 extract Substances 0.000 title claims abstract description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 74
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000002131 composite material Substances 0.000 claims abstract description 33
- 241000208125 Nicotiana Species 0.000 claims abstract description 32
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 32
- 238000002156 mixing Methods 0.000 claims abstract description 31
- 150000001875 compounds Chemical class 0.000 claims abstract description 28
- 238000004898 kneading Methods 0.000 claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 19
- 230000004151 fermentation Effects 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 7
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 6
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229930186217 Glycolipid Natural products 0.000 claims abstract description 6
- 229920002472 Starch Polymers 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 238000010298 pulverizing process Methods 0.000 claims abstract description 6
- 239000008107 starch Substances 0.000 claims abstract description 6
- 235000019698 starch Nutrition 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 46
- 239000000047 product Substances 0.000 claims description 34
- 239000000203 mixture Substances 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 230000001580 bacterial effect Effects 0.000 claims description 28
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 239000000839 emulsion Substances 0.000 claims description 15
- 241000228212 Aspergillus Species 0.000 claims description 11
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 11
- 241000266272 Acidithiobacillus Species 0.000 claims description 10
- 241000589516 Pseudomonas Species 0.000 claims description 10
- 230000001804 emulsifying effect Effects 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 230000003471 anti-radiation Effects 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 7
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 6
- 229920001661 Chitosan Polymers 0.000 claims description 6
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 6
- 230000003712 anti-aging effect Effects 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 6
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 6
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 6
- 235000008390 olive oil Nutrition 0.000 claims description 6
- 239000004006 olive oil Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 229940083466 soybean lecithin Drugs 0.000 claims description 3
- 229940092665 tea leaf extract Drugs 0.000 claims description 3
- 241001122767 Theaceae Species 0.000 claims 13
- 230000003028 elevating effect Effects 0.000 claims 1
- 244000269722 Thea sinensis Species 0.000 abstract description 79
- 238000002360 preparation method Methods 0.000 abstract description 8
- 230000004071 biological effect Effects 0.000 abstract description 2
- 238000005260 corrosion Methods 0.000 abstract description 2
- 230000007797 corrosion Effects 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 235000013616 tea Nutrition 0.000 description 73
- 230000000052 comparative effect Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 229910052785 arsenic Inorganic materials 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000008347 soybean phospholipid Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000002516 radical scavenger Substances 0.000 description 2
- 229940055329 tobacco leaf extract Drugs 0.000 description 2
- 235000015911 Amorpha canescens Nutrition 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000209507 Camellia Species 0.000 description 1
- 244000107780 Capraria biflora Species 0.000 description 1
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 241000589248 Legionella Species 0.000 description 1
- 208000007764 Legionnaires' Disease Diseases 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000013629 Lippia javanica Nutrition 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 241001646828 Platostoma chinense Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229940045110 chitosan Drugs 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000018597 common camellia Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940094952 green tea extract Drugs 0.000 description 1
- 235000020688 green tea extract Nutrition 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/738—Cyclodextrins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Dispersion Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明属于茶叶深加工技术领域,尤其是一种茶叶复合提取物及其制备和应用。所述茶叶复合提取物是采用如下方法制成:(1)将老叶茶叶、烟叶分别置于萎凋槽中萎凋,冷却;(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入淀粉轻揉后,加入复合菌液A处理,制得一次发酵物;(3)将一次发酵物再次轻揉后,加到乙醇溶液中浸泡,捞出,采用蒸汽处理后干燥,制得干燥物;(4)将干燥物粉碎,加入海藻糖脂、复合菌液B处理,灭菌,制得二次发酵物;(5)向二次发酵物中加入50%乙醇溶液,搅拌升温提取,过滤、纳滤,制得提取液;浓缩、干燥后,即得茶叶复合提取物。本发明制备的茶叶复合提取物,具有生物活性高、稳定性强、防腐性能好的特点。
Description
技术领域
本发明属于茶叶深加工技术领域,尤其是一种茶叶复合提取物及应用。
背景技术
茶叶为山茶科植物茶(Camellia sinensis L.)的芽叶,具有独特的营养价值和保健功能,在东汉《神农本草》和明代《本草纲目》中均详细记载了茶叶的药用功效。现代医学研究表明,茶叶含有多种活性成分如茶多酚、茶多糖、生物碱、维生素类、矿物质、微量元素等,其中茶多酚为茶叶中有保健功能的主要成分之一。茶多酚具有清除自由基、抗氧化、抗衰老、抗癌变、防辐射、降血脂和胆固醇、抗动脉粥样硬化以及抑菌等作用。其中茶多酚在体内和体外均具有一定的抗菌和抗炎作用,尤其在抗绿脓杆菌、抑制军团杆菌、百日咳杆菌、杀灭肠道致病菌等方面具有很好的效果。
我国茶叶种植面积位居世界前列,是世界主要的茶叶出口国门。我国西南地区是原始野生茶树主要集中地区,当属茶树的原产地,有悠久的种茶历史,发展当地茶叶是当地农民脱贫致富的骨干项目。然而目前我国茶叶资源浪费比较严重,一般只采春茶,或采少量秋茶供于茶饮品使用,对于大量的老叶茶叶都没有被利用而全部废弃。如何有效地对老叶茶叶进行开发利用,这对于茶叶产业的发展具有重要和深远的意义。老叶茶叶生产时间较长,含有较高重金属素元素,这大大限制了老叶茶叶的应用。
烟草也称仙草,是一味传统的中药材,它的无机成分包括水、矿质元素;有机成分主要包括糖类、生物碱、杂环类、色素、酚类、萜类、有机酸类、脂质类、醇类、醛酮类等。其中,烟草中有机成分具有抗菌、抗病毒、抗肿瘤、抗氧化活性、清除体内自由基等多种功能。现已经被广泛用于抗菌领域。
现已有不少关于茶提取物在抗辐射护肤品方面的应用,但其茶提取物多以成品茶制成,成本加高;而且目前还没有关于茶与烟叶复合在抗辐射护肤品方面的应用。如CN201810352206.9的专利公开了一种及一种抗辐射制剂及其应用,由珍珠粉提取物和绿茶提取物与水复配而成,上述物质复配形成的溶液浓度为0.1-8.0mg/l。
发明内容
为了解决现有技术中存在的上述技术问题,本发明提供了一种茶叶复合提取物及其制备和应用,具体是通过以下技术方案实现的:
一种茶叶复合提取物,是由老叶茶叶和烟叶按6-10:1-3的质量比混合提取制成。
优选地,所述茶叶复合提取物,具体采用如下的方法制成:
(1)将老叶茶叶、烟叶分别置于萎凋槽中,采用30-35℃的热风萎凋,冷却至常温;
(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入其质量0.5-1%的淀粉混匀,得到混合物A;将混合物A放入揉捻机中轻揉30-40min后,加入质量0.01-0.02w%的复合菌液A混匀,在25-30℃下处理6-8h,制得一次发酵物;
(3)将一次发酵物再放入揉捻机中轻揉8-10min后,加到乙醇溶液中浸泡处理3-5min,捞出,采用200-220℃蒸汽处理20-30s,再在50-60℃下干燥至含水量低于20%,制得干燥物;
(4)将干燥物粉碎,加入其质量0.01-0.02%的海藻糖脂,控制其含水率为30-40%,制得混合物B;向混合物B中加入其质量0.03-0.04%的复合菌液B混匀,在25-30℃下处理3-4h,灭菌,制得二次发酵物;
(5)向二次发酵物中加入其质量8-10倍的50%乙醇溶液制成混合溶液,搅拌升温提取,过滤;将滤液再进行纳滤,制得提取液;将提取液回减压回收乙醇后,冷冻干燥至含水率低于10%,即得茶叶复合提取物。
优选地,所述步骤(1),老叶茶叶萎凋至含水率为60-70%;烟叶萎凋至含水量为30-40%。
优选地,所述复合菌液A为嗜酸硫杆菌、解淀粉芽孢杆菌的混合菌液;其中嗜酸硫杆菌的浓度为2-4×106cfu/ml,解淀粉芽孢杆菌的浓度为4-6×104cfu/ml。
优选地,所述步骤(3),所述乙醇溶液中含有40-50w%乙醇、柠檬酸10-15w%;所述乙醇溶液的温度为35-40℃。
优选地,所述复合菌液B为假单孢菌、曲霉菌的混合菌液;其中曲霉菌的浓度为3-5×106cfu/ml,假单孢菌的浓度为1-2×104cfu/ml。
优选地,所述步骤(5),搅拌升温提取是将混合溶液以1-2℃/min搅拌升温至60-70℃,保温提取20-25min。
本发明还提供了一种茶叶复合提取物在抗辐射、抗衰老乳液中的应用,按重量份取1-2份茶叶复合提取物、0.1-0.2份微晶纤维素、1-2份壳聚糖、3-4份大豆磷脂、0.5-1份橄榄油、60-70份水;然后将各原料混合送入到乳化机中以10000r/min的转速剪切乳化5-6min,即得乳液。
本发明的有益效果在于:
本发明在茶叶复合提取物制备过程中,先采用复合菌液A对轻揉后的老叶茶叶、烟叶进行处理,复合菌液A中的嗜酸硫杆菌、解淀粉芽孢杆菌在发酵处理过程中能与老叶茶叶以及烟叶中的重金素元素、残留农药等发生相互作用,促使重金元素的溶出及残留农药的降解;然后采用含有柠檬酸的乙醇溶液对揉捻后的茶叶、烟叶进行浸泡,能有效成浸出其中的重金素元素。采用假单孢菌、曲霉菌的复合菌液B对复合物B进行发酵处理,能有效提高老叶茶叶、烟叶中生物活性物质的溶出率,促进生物活性物质的转化,提高其活性。过滤后采用纳滤进行分离,能有效去滤液中的大分子物质和重金属离子。
本发明制备的茶叶复合提取物,具有生物活性高、稳定性强、防腐性能好的特点;将茶叶复合提取物与微晶纤维素、壳聚糖、大豆磷脂、橄榄油等物质混合制成的天然乳液,具有良好的抗辐射、抗衰老功效。
具体实施方式
下面结合具体的实施方式来对本发明的技术方案做进一步的限定,但要求保护的范围不仅局限于所作的描述。
实施例1
一种茶叶复合提取物,具体采用如下的方法制成:
(1)按6-10:1-3的质量比取老叶茶叶、烟叶分别置于萎凋槽中,采用30-35℃的热风萎凋;老叶茶叶萎凋至含水率为60-70%;烟叶萎凋至含水量为30-40%;萎凋后冷却至常温;
(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入其质量0.5-1%的淀粉混匀,得到混合物A;将混合物A放入揉捻机中轻揉30-40min后,加入质量0.01-0.02w%的复合菌液A混匀,在25-30℃下处理6-8h,制得一次发酵物;
(3)将一次发酵物再放入揉捻机中轻揉8-10min后,加到乙醇溶液中浸泡处理3-5min,捞出,采用200-220℃蒸汽处理20-30s,再在50-60℃下干燥至含水量低于20%,制得干燥物;
(4)将干燥物粉碎,加入其质量0.01-0.02%的海藻糖脂,控制其含水率为30-40%,制得混合物B;向混合物B中加入其质量0.03-0.04%的复合菌液B混匀,在25-30℃下处理3-4h,灭菌,制得二次发酵物;
(5)向二次发酵物中加入其质量8-10倍的50%乙醇溶液制成混合溶液,搅拌升温提取,过滤;将滤液再进行纳滤,制得提取液;将提取液回减压回收乙醇后,冷冻干燥至含水率低于10%,即得茶叶复合提取物。
所述复合菌液A为嗜酸硫杆菌、解淀粉芽孢杆菌的混合菌液;其中嗜酸硫杆菌的浓度为2-4×106cfu/ml,解淀粉芽孢杆菌的浓度为4-6×104cfu/ml。
所述步骤(3),所述乙醇溶液中含有40-50w%乙醇、柠檬酸10-15w%;所述乙醇溶液的温度为35-40℃。
所述复合菌液B为假单孢菌、曲霉菌的混合菌液;其中曲霉菌的浓度为3-5×106cfu/ml,假单孢菌的浓度为1-2×104cfu/ml。
所述步骤(5),搅拌升温提取是将混合溶液以1-2℃/min搅拌升温至60-70℃,保温提取20-25min。
取上述制成的茶叶复合提取物,制备抗辐射、抗衰老乳液,包括以下过程:
按重量份取1-2份茶叶复合提取物、0.1-0.2份微晶纤维素、1-2份壳聚糖、3-4份大豆磷脂、0.5-1份橄榄油、60-70份水;然后将各原料混合送入到乳化机中以10000r/min的转速剪切乳化5-6min,即得乳液。
实施例2
一种茶叶复合提取物,具体采用如下的方法制成:
(1)按6-10:1-3的质量比取老叶茶叶、烟叶分别置于萎凋槽中,采用30-35℃的热风萎凋;老叶茶叶萎凋至含水率为60-70%;烟叶萎凋至含水量为30-40%;萎凋后冷却至常温;
(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入其质量0.5-1%的淀粉混匀,得到混合物A;将混合物A放入揉捻机中轻揉30-40min后,加入质量0.01-0.02w%的复合菌液A混匀,在25-30℃下处理6-8h,制得一次发酵物;
(3)将一次发酵物再放入揉捻机中轻揉8-10min后,加到乙醇溶液中浸泡处理3-5min,捞出,采用200-220℃蒸汽处理20-30s,再在50-60℃下干燥至含水量低于20%,制得干燥物;
(4)将干燥物粉碎,加入其质量0.01-0.02%的海藻糖脂,控制其含水率为30-40%,制得混合物B;向混合物B中加入其质量0.03-0.04%的复合菌液B混匀,在25-30℃下处理3-4h,灭菌,制得二次发酵物;
(5)向二次发酵物中加入其质量8-10倍的50%乙醇溶液制成混合溶液,搅拌升温提取,过滤;将滤液再进行纳滤,制得提取液;将提取液回减压回收乙醇后,冷冻干燥至含水率低于10%,即得茶叶复合提取物。
所述复合菌液A为嗜酸硫杆菌、解淀粉芽孢杆菌的混合菌液;其中嗜酸硫杆菌的浓度为2-4×106cfu/ml,解淀粉芽孢杆菌的浓度为4-6×104cfu/ml。
所述步骤(3),所述乙醇溶液中含有40-50w%乙醇、柠檬酸10-15w%;所述乙醇溶液的温度为35-40℃。
所述复合菌液B为假单孢菌、曲霉菌的混合菌液;其中曲霉菌的浓度为3-5×106cfu/ml,假单孢菌的浓度为1-2×104cfu/ml。
所述步骤(5),搅拌升温提取是将混合溶液以1-2℃/min搅拌升温至60-70℃,保温提取20-25min。
取上述制成的茶叶复合提取物,制备抗辐射、抗衰老乳液,包括以下过程:
按重量份取1-2份茶叶复合提取物、0.1-0.2份微晶纤维素、1-2份壳聚糖、3-4份大豆磷脂、0.5-1份橄榄油、60-70份水;然后将各原料混合送入到乳化机中以10000r/min的转速剪切乳化5-6min,即得乳液。
实施例3
一种茶叶复合提取物,具体采用如下的方法制成:
(1)按6-10:1-3的质量比取老叶茶叶、烟叶分别置于萎凋槽中,采用30-35℃的热风萎凋;老叶茶叶萎凋至含水率为60-70%;烟叶萎凋至含水量为30-40%;萎凋后冷却至常温;
(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入其质量0.5-1%的淀粉混匀,得到混合物A;将混合物A放入揉捻机中轻揉30-40min后,加入质量0.01-0.02w%的复合菌液A混匀,在25-30℃下处理6-8h,制得一次发酵物;
(3)将一次发酵物再放入揉捻机中轻揉8-10min后,加到乙醇溶液中浸泡处理3-5min,捞出,采用200-220℃蒸汽处理20-30s,再在50-60℃下干燥至含水量低于20%,制得干燥物;
(4)将干燥物粉碎,加入其质量0.01-0.02%的海藻糖脂,控制其含水率为30-40%,制得混合物B;向混合物B中加入其质量0.03-0.04%的复合菌液B混匀,在25-30℃下处理3-4h,灭菌,制得二次发酵物;
(5)向二次发酵物中加入其质量8-10倍的50%乙醇溶液制成混合溶液,搅拌升温提取,过滤;将滤液再进行纳滤,制得提取液;将提取液回减压回收乙醇后,冷冻干燥至含水率低于10%,即得茶叶复合提取物。
所述复合菌液A为嗜酸硫杆菌、解淀粉芽孢杆菌的混合菌液;其中嗜酸硫杆菌的浓度为2-4×106cfu/ml,解淀粉芽孢杆菌的浓度为4-6×104cfu/ml。
所述步骤(3),所述乙醇溶液中含有40-50w%乙醇、柠檬酸10-15w%;所述乙醇溶液的温度为35-40℃。
所述复合菌液B为假单孢菌、曲霉菌的混合菌液;其中曲霉菌的浓度为3-5×106cfu/ml,假单孢菌的浓度为1-2×104cfu/ml。
所述步骤(5),搅拌升温提取是将混合溶液以1-2℃/min搅拌升温至60-70℃,保温提取20-25min。
取上述制成的茶叶复合提取物,制备抗辐射、抗衰老乳液,包括以下过程:
按重量份取1-2份茶叶复合提取物、0.1-0.2份微晶纤维素、1-2份壳聚糖、3-4份大豆磷脂、0.5-1份橄榄油、60-70份水;然后将各原料混合送入到乳化机中以10000r/min的转速剪切乳化5-6min,即得乳液。
对比例1
对比例1与实施例1的区别在于没有步骤(4),是将干燥物粉碎后直接加入50%乙醇溶液进行提取;其余过程相同。
对比例2
对比例2与实施例1的区别在于没有步骤(2),是将混合物轻揉30-40min后直接加到步骤(3)的乙醇溶液中浸泡;其余过程相同。
对比例3茶叶提取物
对比例3与实施例1的区别在于提取原料只有老叶茶叶;其余过程相同。
对比例4烟叶提取物
对比例4与实施例1的区别在于提取原料只有烟叶;其余过程相同。
实验例1
取实施例1-3和对比例1-2制备的茶叶复合提取物,对比例3制备的茶叶提取物,对比例4制备的烟叶提取物;测量其中的Pb、As含量;As检测按照GB/T 5009.11-2003食品中总砷及无机砷的测定进行;Pb检测按照GB/T 5009.12-2010。测量结果如表1所示:
表1
实验例2自由基清除试验
试验原理:DPPH·在有机溶剂中是一种稳定的自由基,其孤对电子在517nm附近有强吸收(显深紫色)。当有机清除剂存在时,孤对电子被配对,吸收消失或减弱,通过测定吸收减弱的程度,可评价自由基清除剂的活性。
试验样品:样品1-7:实施例1-7制备的天然乳液。
试验方法:分别待测品溶液2mL、浓度为120μmoL/L的DPPH溶液2mL,先后加入同一具塞试管中,摇匀;室温下静置30min,于517nm波长下测定样品吸光度。
抑制率:K%=[1-(A0-A1)/A2]×100%
A0:2mLDPPH溶液+2mL待测品溶液的吸光度
A1:2mL待测品溶液+2mL溶剂的吸光度
A2:2mLDPPH溶液+2mL溶剂的吸光度
待测品溶液是将乳液用50%乙醇溶液稀释成浓度为0.5mg/ml的溶液;溶剂为50%乙醇。
分别在0个月、6个月、12个月检测其对DPPH的清除率,保存方法:玻璃瓶中,密闭保存,保存环境为室内常规放置,避免阳光直射。
实验结果如表1所所示:
表1
实施例1 | 实施例2 | 实施例3 | 对比例1 | 对比例2 | 对比例3 | 对比例4 | |
0个月 | 95.1% | 95.3% | 94.8% | 82.1% | 93.1% | 91.4 | 87.3% |
6个月 | 91.4% | 91.2% | 90.7% | 74.9% | 87.7% | 85.4% | 81.4% |
12个月 | 86.7% | 87.1% | 85.9% | 64.1% | 81.3% | 74.9% | 70.20% |
从上表中可知,实施例1-3制备的的乳液对自由基的清除率高于对比例1-4,有显著性差异的,说明本发明制备的乳液有更强的自由基清除率,抗辐射、抗衰老效果更好;且自由基清除率随着时间延长的下降率低于对比例对比例1-4,说明本发明实施例1-3制备的乳液其稳定性强于对比例1-4制备的乳液。
在此有必要指出的是,以上实施例和试验例仅限于对本发明的技术方案做进一步的阐述和理解,不能理解为对本发明的技术方案做进一步的限定,本领域技术人员作出的非突出实质性特征和显著进步的发明创造,仍然属于本发明的保护范畴。
Claims (8)
1.一种茶叶复合提取物,其特征在于,是由老叶茶叶和烟叶按6-10:1-3的质量比混合提取制成。
2.如权利要求1所述的茶叶复合提取物,其特征在于,具体采用如下的方法制成:
(1)将老叶茶叶、烟叶分别置于萎凋槽中,采用30-35℃的热风萎凋,冷却至常温;
(2)将萎凋冷却后的老叶茶叶与烟叶混合,加入其质量0.5-1%的淀粉混匀,得到混合物A;将混合物A放入揉捻机中轻揉30-40min后,加入质量0.01-0.02w%的复合菌液A混匀,在25-30℃下处理6-8h,制得一次发酵物;
(3)将一次发酵物再放入揉捻机中轻揉8-10min后,加到乙醇溶液中浸泡处理3-5min,捞出,采用200-220℃蒸汽处理20-30s,再在50-60℃下干燥至含水量低于20%,制得干燥物;
(4)将干燥物粉碎,加入其质量0.01-0.02%的海藻糖脂,控制其含水率为30-40%,制得混合物B;向混合物B中加入其质量0.03-0.04%的复合菌液B混匀,在25-30℃下处理3-4h,灭菌,制得二次发酵物;
(5)向二次发酵物中加入其质量8-10倍的50%乙醇溶液制成混合溶液,搅拌升温提取,过滤;将滤液再进行纳滤,制得提取液;将提取液回减压回收乙醇后,冷冻干燥至含水率低于10%,即得茶叶复合提取物。
3.如权利要求2所述的茶叶复合提取物,其特征在于,所述步骤(1),老叶茶叶萎凋至含水率为60-70%;烟叶萎凋至含水量为30-40%。
4.如权利要求2所述的茶叶复合提取物,其特征在于,所述复合菌液A为嗜酸硫杆菌、解淀粉芽孢杆菌的混合菌液;其中嗜酸硫杆菌的浓度为2-4×106cfu/ml,解淀粉芽孢杆菌的浓度为4-6×104cfu/ml。
5.如权利要求2所述的茶叶复合提取物,其特征在于,所述步骤(3),所述乙醇溶液中含有40-50w%乙醇、柠檬酸10-15w%;所述乙醇溶液的温度为35-40℃。
6.如权利要求2所述的茶叶复合提取物,其特征在于,所述复合菌液B为假单孢菌、曲霉菌的混合菌液;其中曲霉菌的浓度为3-5×106cfu/ml,假单孢菌的浓度为1-2×104cfu/ml。
7.如权利要求2所述的茶叶复合提取物,其特征在于,所述步骤(5),搅拌升温提取是将混合溶液以1-2℃/min搅拌升温至60-70℃,保温提取20-25min。
8.一种如权利要求1-7任一项所述的茶叶复合提取物在抗辐射、抗衰老乳液中的应用,其特征在于,按重量份取1-2份茶叶复合提取物、0.1-0.2份微晶纤维素、1-2份壳聚糖、3-4份大豆磷脂、0.5-1份橄榄油、60-70份水;然后将各原料混合送入到乳化机中以10000r/min的转速剪切乳化5-6min,即得乳液。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911202476.2A CN110974741B (zh) | 2019-11-29 | 2019-11-29 | 一种茶叶复提取物及应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911202476.2A CN110974741B (zh) | 2019-11-29 | 2019-11-29 | 一种茶叶复提取物及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110974741A true CN110974741A (zh) | 2020-04-10 |
CN110974741B CN110974741B (zh) | 2022-06-28 |
Family
ID=70088367
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201911202476.2A Active CN110974741B (zh) | 2019-11-29 | 2019-11-29 | 一种茶叶复提取物及应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110974741B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112844170A (zh) * | 2020-12-22 | 2021-05-28 | 福建安溪铁观音集团股份有限公司 | 一种茶叶、茶梗多成分模块化同步提取生产线 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140356295A1 (en) * | 2013-06-03 | 2014-12-04 | R.J. Reynolds Tobacco Company | Cosmetic compositions comprising tobacco seed-derived component |
US20170312216A1 (en) * | 2014-10-27 | 2017-11-02 | Danjoungbio Co., Ltd. | Cosmetic composition for improving anti-oxidation, anti-inflammation and atopic skin by using fermented black tea liquid as effective ingredient and method of preparing the same |
CN107455501A (zh) * | 2017-09-19 | 2017-12-12 | 贵州阳春白雪茶业有限公司 | 茶叶发酵工艺 |
CN108524352A (zh) * | 2018-04-19 | 2018-09-14 | 绍兴文理学院 | 一种抗辐射制剂及其应用 |
CN108690727A (zh) * | 2018-07-25 | 2018-10-23 | 贵州夜郎古国生物科技有限公司 | 一种油茶精油的制备方法 |
CN109044859A (zh) * | 2018-10-18 | 2018-12-21 | 福建省惜缘生态农业开发有限公司 | 一种乌龙茶祛痘水及其制备方法 |
-
2019
- 2019-11-29 CN CN201911202476.2A patent/CN110974741B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140356295A1 (en) * | 2013-06-03 | 2014-12-04 | R.J. Reynolds Tobacco Company | Cosmetic compositions comprising tobacco seed-derived component |
US20170312216A1 (en) * | 2014-10-27 | 2017-11-02 | Danjoungbio Co., Ltd. | Cosmetic composition for improving anti-oxidation, anti-inflammation and atopic skin by using fermented black tea liquid as effective ingredient and method of preparing the same |
CN107455501A (zh) * | 2017-09-19 | 2017-12-12 | 贵州阳春白雪茶业有限公司 | 茶叶发酵工艺 |
CN108524352A (zh) * | 2018-04-19 | 2018-09-14 | 绍兴文理学院 | 一种抗辐射制剂及其应用 |
CN108690727A (zh) * | 2018-07-25 | 2018-10-23 | 贵州夜郎古国生物科技有限公司 | 一种油茶精油的制备方法 |
CN109044859A (zh) * | 2018-10-18 | 2018-12-21 | 福建省惜缘生态农业开发有限公司 | 一种乌龙茶祛痘水及其制备方法 |
Non-Patent Citations (2)
Title |
---|
RU ET AL.: "In Vitro Antioxidant Properties of Flavonoids and Polysaccharides Extract from Tobacco (Nicotiana tabacum L.) Leaves", 《MOLECULES》 * |
李晓芹: "烟草主要功能成分的联合提取及抗氧化活性研究", 《中国优秀博硕士学位论文全文数据库(硕士)工程科技Ⅰ辑》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112844170A (zh) * | 2020-12-22 | 2021-05-28 | 福建安溪铁观音集团股份有限公司 | 一种茶叶、茶梗多成分模块化同步提取生产线 |
Also Published As
Publication number | Publication date |
---|---|
CN110974741B (zh) | 2022-06-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Banerjee et al. | Efficient extraction strategies of tea (Camellia sinensis) biomolecules | |
CN103190498B (zh) | 一种金银花茶的加工方法 | |
CN106035845B (zh) | 一种铁皮石斛叶茶饮料的加工方法 | |
JP5638528B2 (ja) | 茶製品を製造する方法 | |
CN103349110B (zh) | 一种提取率高、有益成分保留完全的活性茶膏及其制备方法 | |
CN106721885A (zh) | 一种食品保鲜剂及其制备方法 | |
CN110974741B (zh) | 一种茶叶复提取物及应用 | |
CN101455257B (zh) | 一种用发酵法制备茶褐素的方法 | |
CN110755318A (zh) | 一种丁香提取物在制备化妆品中的应用 | |
CN106720685A (zh) | 一种青钱柳速溶茶及其制备方法 | |
CN108835328A (zh) | 一种发酵茶的生产方法 | |
CN103621742B (zh) | 一种蔷薇花茶、其制备方法和用途 | |
CN107823008B (zh) | 一种从天然植物中提取的香精添加剂及其制备方法 | |
CN116235919A (zh) | 一种植物源防霉固形物制备方法及相应固形物 | |
CN102258099A (zh) | 一种紫娟普洱茶茶褐素及其应用 | |
CN108477350A (zh) | 一种芍药花青素茶及其制备方法 | |
CN113951346B (zh) | 一种茶饮料及其制备方法 | |
CN113249359B (zh) | 一种利用酶提取桑叶功能成分的方法 | |
CN108685951A (zh) | 一种无刺蜂蜂蜜提取物及其提取方法和应用 | |
CN114468086A (zh) | 一种刺梨红茶及其制备方法 | |
KR20190052762A (ko) | 녹차 농축액을 함유하는 건강 배즙의 제조방법 | |
CN102058731B (zh) | 荆条叶及果实提取物的制备及其抗炎的医用用途 | |
CN111436507A (zh) | 一种紫苏茯砖茶及其制备方法 | |
CN112206286B (zh) | 黑青稞黑藜麦组合物在制备抗氧化产品中的用途 | |
Mesfin et al. | The effect of roasting on the antioxidant and antibacterial capacity of coffee cherry husk extract |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A tea complex extract and its application Effective date of registration: 20231218 Granted publication date: 20220628 Pledgee: Bank of Guizhou Co.,Ltd. Shiqian Sub branch Pledgor: SHIQIAN COUNTY YIZHOU TRIBUTE TEA Ltd. Registration number: Y2023520000073 |