CN110934221A - Preparation method of tenebrio molitor protein powder - Google Patents
Preparation method of tenebrio molitor protein powder Download PDFInfo
- Publication number
- CN110934221A CN110934221A CN201811111103.XA CN201811111103A CN110934221A CN 110934221 A CN110934221 A CN 110934221A CN 201811111103 A CN201811111103 A CN 201811111103A CN 110934221 A CN110934221 A CN 110934221A
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- CN
- China
- Prior art keywords
- protein
- crude
- tenebrio molitor
- yellow
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000254109 Tenebrio molitor Species 0.000 title claims abstract description 56
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 33
- 239000000843 powder Substances 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 9
- 101710109488 Salt stress-induced protein Proteins 0.000 claims abstract description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 108091005804 Peptidases Proteins 0.000 claims abstract description 4
- 239000004365 Protease Substances 0.000 claims abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 4
- 238000011033 desalting Methods 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000004537 pulping Methods 0.000 claims abstract description 4
- 239000011780 sodium chloride Substances 0.000 claims abstract description 4
- 230000000415 inactivating effect Effects 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract 1
- 230000000050 nutritive effect Effects 0.000 abstract 1
- 241000282414 Homo sapiens Species 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 3
- 241000382353 Pupa Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000004350 Strabismus Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of health-care food, in particular to a preparation method of tenebrio molitor protein powder, which comprises the following steps: (1) selecting fresh yellow mealworms, cleaning, removing impurities and cleaning for later use; (2) adding water into yellow mealworm, pulping, adding sodium chloride solution into yellow mealworm liquid, homogenizing for 3-5 minutes, extracting for 1-2 hours, centrifuging, and respectively collecting filtrate and filter residue for later use; (3) adjusting the pH of the filtrate to 4.0-4.5, separating out crude yellow meal worm protein from the filtrate, and centrifuging to obtain crude yellow meal worm salt protein; (4) adding distilled water into the filter residue, adjusting pH to 6.0-10.0, homogenizing for 3-5 min, adding protease for enzymolysis for 1-1.5 hr, heating in water bath to inactivate enzyme, filtering, and collecting the filter residue to obtain enzymolysis crude protein; (5) mixing the crude salt protein and the enzymolysis crude protein, dialyzing and desalting to obtain desalted protein, and drying to constant weight to obtain the salt-free protein; the tenebrio molitor protein powder is high in nutritive value and can be used in various health-care foods.
Description
Technical Field
The invention relates to the technical field of health-care food, in particular to a preparation method of tenebrio molitor protein powder.
Background
The yellow mealworms are commonly called bread worms, the body surfaces of larvae are yellow, the yellow mealworms cultivation technology is not difficult, and as long as people know that the yellow mealworms are often gathered in grain piles in grain warehouses in groups, the yellow mealworms are essentially pests and are one of the most ideal insects for artificial cultivation, because the yellow mealworms are rich in high protein, mineral substances, seventeen amino acids and the like, and the pupas of the yellow mealworms are high-grade dishes, are delicious green harmless food with the most abundant nutrition, and are rich in high protein. In life, people need low fat and a plurality of vitamins indispensable to human bodies. People are known to pursue green insect pupa vegetables to be ready to be a fashion and accept people's squint under the principles of reasonable diet and balanced nutrition, and the insect pupa vegetables are excellent protein food.
The yellow mealworm has rich protein content, complete types of amino acid and reasonable composition; the unique multiple nutrients of the yellow mealworm are easy to digest and absorb, the efficacies of improving human immunity, resisting fatigue, reducing blood fat, resisting cancer, promoting metabolism and the like are achieved, and the SOD extracted from the yellow mealworm is used for resisting aging, preventing wrinkles, maintaining beauty and keeping young, and the effect is prior to the existing market products. The yellow mealworm has high medicinal value, and the protein peptide extracted from the yellow mealworm has the function of improving the immunity of human bodies. At present, the yellow mealworms are mainly used as animal feed, and rarely used for human food, so that the development of a preparation method of yellow mealworm protein powder has very important significance for developing the high-end product market of the yellow mealworms.
Disclosure of Invention
The invention aims to provide a preparation method of tenebrio molitor protein powder, aiming at the problems that the tenebrio molitor has high nutritional value, particularly has rich protein content and can be used as a high-quality protein supplement for human beings, and no tenebrio molitor protein powder is processed by using the tenebrio molitor at present.
The invention relates to a preparation method of tenebrio molitor protein powder, which comprises the following steps:
(1) selecting fresh tenebrio molitor which is complete in body form and fresh and has no rot, cleaning to remove impurities, and cleaning for later use;
(2) adding 3-5 times of water into cleaned yellow mealworm, pulping, adding 0.5-2.0% of sodium chloride solution with the volume of 3-10 times of that of yellow mealworm slurry, homogenizing in a homogenizer for 3-5 minutes, extracting for 1-2 hours, and centrifuging; respectively collecting filtrate and filter residue for later use;
(3) adjusting the pH of the filtrate to 4.0-4.5, separating out crude yellow meal worm protein from the filtrate, and centrifuging to obtain crude yellow meal worm salt protein;
(4) adding 3-5 times of distilled water into the filter residue, adjusting pH to 6.0-10.0, homogenizing in a homogenizer for 3-5 min, adding protease, performing enzymolysis at 40-50 deg.C for 1-1.5 hr, heating in water bath to above 70 deg.C for inactivating enzyme, filtering, and collecting the filter residue to obtain enzymolysis crude protein;
(5) mixing the crude salt protein and the enzymolysis crude protein, dialyzing and desalting to obtain desalted protein, and drying to constant weight to obtain the tenebrio molitor protein powder.
The tenebrio molitor protein powder is reasonable in preparation method and simple in production process, and by utilizing the characteristic that the tenebrio molitor contains high protein, the high-quality tenebrio molitor protein powder is obtained by comprehensively extracting the protein in the tenebrio molitor through salt hydrolysis and enzymolysis; the tenebrio molitor protein powder can be used as a protein additive in various health-care foods, and has very important significance for expanding new protein resources and promoting the development of the tenebrio molitor breeding industry.
Detailed Description
Example 1
A preparation method of tenebrio molitor protein powder comprises the following steps:
(1) selecting fresh tenebrio molitor which is complete in body form and fresh and has no rot, cleaning to remove impurities, and cleaning for later use;
(2) adding 4 times of water into the cleaned yellow mealworms for pulping, adding 1.5 mass percent of sodium chloride solution with the volume 5 times of the yellow mealworms into the yellow mealworms pulp, homogenizing in a homogenizer for 4 minutes, extracting for 1.5 hours, and centrifuging; respectively collecting filtrate and filter residue for later use;
(3) adjusting the pH of the filtrate to 4.2, separating out tenebrio molitor crude protein from the filtrate, and centrifuging to obtain tenebrio molitor crude salt protein;
(4) adding 4 times of distilled water into the filter residue, adjusting pH to 8.0, homogenizing in a homogenizer for 4 min, adding protease, performing enzymolysis at 45 deg.C for 1.5 hr, heating in water bath to above 70 deg.C, inactivating enzyme, filtering, and collecting the filter residue to obtain enzymolysis crude protein;
(5) mixing the crude salt protein and the enzymolysis crude protein, dialyzing and desalting to obtain desalted protein, and drying to constant weight to obtain the tenebrio molitor protein powder.
Claims (1)
1. The preparation method of the tenebrio molitor protein powder is characterized by comprising the following steps:
(1) selecting fresh tenebrio molitor which is complete in body form and fresh and has no rot, cleaning to remove impurities, and cleaning for later use;
(2) adding 3-5 times of water into cleaned yellow mealworm, pulping, adding 0.5-2.0% of sodium chloride solution with the volume of 3-10 times of that of yellow mealworm slurry, homogenizing in a homogenizer for 3-5 minutes, extracting for 1-2 hours, and centrifuging; respectively collecting filtrate and filter residue for later use;
(3) adjusting the pH of the filtrate to 4.0-4.5, separating out crude yellow meal worm protein from the filtrate, and centrifuging to obtain crude yellow meal worm salt protein;
(4) adding 3-5 times of distilled water into the filter residue, adjusting pH to 6.0-10.0, homogenizing in a homogenizer for 3-5 min, adding protease, performing enzymolysis at 40-50 deg.C for 1-1.5 hr, heating in water bath to above 70 deg.C for inactivating enzyme, filtering, and collecting the filter residue to obtain enzymolysis crude protein;
(5) mixing the crude salt protein and the enzymolysis crude protein, dialyzing and desalting to obtain desalted protein, and drying to constant weight to obtain the tenebrio molitor protein powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811111103.XA CN110934221A (en) | 2018-09-22 | 2018-09-22 | Preparation method of tenebrio molitor protein powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811111103.XA CN110934221A (en) | 2018-09-22 | 2018-09-22 | Preparation method of tenebrio molitor protein powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110934221A true CN110934221A (en) | 2020-03-31 |
Family
ID=69905349
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811111103.XA Pending CN110934221A (en) | 2018-09-22 | 2018-09-22 | Preparation method of tenebrio molitor protein powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110934221A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768888A (en) * | 2021-07-29 | 2021-12-10 | 大连百利天华制药有限公司 | Preparation method and application of spleen aminopeptide freeze-dried powder |
-
2018
- 2018-09-22 CN CN201811111103.XA patent/CN110934221A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768888A (en) * | 2021-07-29 | 2021-12-10 | 大连百利天华制药有限公司 | Preparation method and application of spleen aminopeptide freeze-dried powder |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200331 |