CN105166434A - Coated feed for control of leech blight and preparation method thereof - Google Patents

Abstract

The invention discloses a coated feed for control of leech blight. The coated feed is prepared from the following raw materials by weight part: 30-40 of peanut bran, 30-35 of freshwater mussel meat, 6-8 of Prunus humilis leaf, 40-50 of pig plasma, 5-8 of cheese, 40-50 of Amaranthus hypochondriacus, 30-40 of Tenebrio molitor, 5-8 of plasma protein powder, and proper amounts of edible oil, oxytetracycline, yeast, Bacillus natto, sodium hydroxide, chitosan and water. The Tenebrio molitor and Amaranthus hypochondriacus in the feed are subjected to extraction, centrifugal fermentation and other processes to prepare protein peptide that is easier to digest and absorb by leeches and has better physiological functions. The feed has fragrant flavor and is easy to store. Oxytetracycline has certain antibacterial activity, can effectively improve the resistance of leeches to diseases after combination with high calcium content Prunus humilis leaf, thus realizing good control of leech blight.

Description

A kind of coating feed preventing and treating leech die back disease and preparation method thereof

Technical field

The present invention relates to a kind of field of feed, particularly a kind of coating feed preventing and treating leech die back disease and preparation method thereof.

Background technology

China utilizes leech nearly one thousand years more Zao than west, and leech begins to remember that history tree is all on the books in " legendary god of farming's book on Chinese herbal medicine " as Chinese medicine.In national academic conference promoting blood circulation and removing blood stasis in 1986, leech is confirmed as one of 35 kinds of Chinese medicines promoting blood circulation and removing blood stasis.Englishman Mike watt finds and the hirudin extracted the 1950's, and a few microgram just can make blood solidify, and it can reduce blood viscosity, alleviation arterial wall spasm, hemangiectasis, stimulates circulation and absorb exudate.Hirudin also can be used for treating miocardial infarction, headstroke, thrombus class angiosis and vascular hypertension.Surgery removes hemostasis after being used for reconstructive operation, is the jinx of the thrombotic diseases such as cardiovascular disease.Leech vitality is extremely strong, substantially disease-free, but strict to water quality requirement.Water body requires that water source is avoided containing strong iron and sulphur matter, can survive in the waters of pH value 6.4-9 without industry and domestic pollution, but can not live in the acid waters that pH value is extremely low.The leech annual 3-6 month breeds, and lays eggs cocoon 2-3, young leech 13-35 bar in each cocoon, most 20 at every turn.

Yellow meal worm is a kind of important storage pest be distributed in all over the world, and be commonly called as bread worm, indoor feeding is extremely easy.Being of high nutritive value of yellow meal worm, have the health-care effects such as antifatigue, anti-ageing, anti-sudden change, adjusting blood lipid and immunity moderation function, wherein to reach 56.58%, 18 seed amino acids complete for crude protein content, its essential amino acids content accounts for 39.14% of total amino acid content, is a kind of protein resource of high-quality.Grain Production of Amaranthus is annual grain, the feed dual-purpose crop of Amaranthaceae Amaranthus.Protein content in Grain Production of Amaranthus amaranth seed and quality are all higher than general cereal, and amino acid proportion balance.Protein is extracted from yellow meal worm and Grain Production of Amaranthus, then for the coating of feed after fermentation.

Summary of the invention

The present invention compensate for the deficiencies in the prior art, provides a kind of coating feed preventing and treating leech die back disease and preparation method thereof.

Technical scheme of the present invention is as follows:

Leech feed of the present invention is made up of the raw material of following weight portion: peanut press pulp 30-40, mussel meat 30-35, leaf of GAIGUO 6-8, Swine plasma 40-50, cheese 5-8, Grain Production of Amaranthus 40-50, yellow meal worm 30-40, SDPP 5-8, edible oil, terramycin, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate;

The concrete steps of the preparation of described leech feed are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 1-2 minute, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) leaf of GAIGUO is added suitable quantity of water and grind to form juice, then stir into pureed after mixing with mussel meat, squeeze into press filtration in filter press, the press filtration rate of rise is 0.1Mpa/30 minute, obtains filtrate and filter residue;

(5) the yellow meal worm sediment of the Grain Production of Amaranthus sediment of step 1, step 2, the filter residue of step 4, peanut press pulp, saccharomycete are mixed, sealing, normal temperature bottom fermentation 20-24 hour, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of step 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dries.

Beneficial effect of the present invention:

In the present invention, SDPP not only can provide a large amount of nutritional needs, the smell of blood of feed can also be increased, take food in a large number when luring leech to eat food, adopt the multiple enzyme system such as Bacillus natto extracellular proteinase and cellulase to be hydrolyzed the protein liquid extracted from yellow meal worm and Grain Production of Amaranthus and be prepared into low molecular protein peptide, low molecular protein peptide has promotion lipid-metabolism, reduces all more important physiological functions such as cholesterol and blood pressure, anti-oxidant, antifatigue, develop immunitypty, promotion wound healing.Again by Grain Production of Amaranthus, yellow meal worm sediment and other composition mixing after fermentation process, not only improve the nutritive value of feed, all right hyperchromic flavouring, improves the appetite of leech.

In feed yellow meal worm and Grain Production of Amaranthus by extracting, the technique such as centrifugal fermentation prepares more easily by the protein peptides that leech digests and assimilates, and has better physiological function, feed taste is fragrant, storage easily.The certain antibacterial activity of terramycin tool, after the leaf of GAIGUO high with calcium content is combined, effectively improves the disease resistance of leech, has the generation of well control leech die back disease.

Specific embodiments

Below in conjunction with following detailed description of the invention, the present invention is described in further detail:

The raw material taking following weight portion (kg) is made: peanut press pulp 34, mussel meat 35, leaf of GAIGUO 7, Swine plasma 46, cheese 7, Grain Production of Amaranthus 45, yellow meal worm 35, SDPP 7, edible oil, terramycin, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate;

The concrete steps of the preparation method of leech feed are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 2 minutes, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) leaf of GAIGUO is added suitable quantity of water and grind to form juice, then stir into pureed after mixing with mussel meat, squeeze into press filtration in filter press, the press filtration rate of rise is 0.1Mpa/30 minute, obtains filtrate and filter residue;

(5) the yellow meal worm sediment of the Grain Production of Amaranthus sediment of step 1, step 2, the filter residue of step 4, peanut press pulp, saccharomycete are mixed, sealing, normal temperature bottom fermentation 22 hours, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of step 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dries.

In order to further illustrate using value of the present invention, implement people and leech is equally divided into experimental group and control group two groups according to age in days, body weight, principle that health status is identical by 200 leech, experimental group feeds leech feed of the present invention, control group feeds traditional leech feed, and experimental period is 3 months, and result of the test is that the feed of experimental group leech is active, survival rate improves 12.8%, efficiency of feed utilization improves 15.2%, and daily gain improves 7.1%, and die back disease incidence reduces by 9.4%.

Claims (2)

1. prevent and treat the coating feed of leech die back disease for one kind, it is characterized in that, be made up of the raw material of following weight portion: peanut press pulp 30-40, mussel meat 30-35, leaf of GAIGUO 6-8, Swine plasma 40-50, cheese 5-8, Grain Production of Amaranthus 40-50, yellow meal worm 30-40, SDPP 5-8, edible oil, terramycin, saccharomycete, Bacillus natto, NaOH, shitosan, water are appropriate.

2. prevent and treat the coating feed of leech die back disease according to claim 1, it is characterized in that, the concrete steps of preparation method are as follows:

(1) Grain Production of Amaranthus is ground into Powdered, adds the water yield of 20 times, with NaOH adjust pH to 9, under 30 DEG C of conditions, vibration insulation 1h, takes out and leaves standstill 1-2 minute, centrifugation 10 minutes, speed per hour is 1500 revs/min, is separated to obtain Grain Production of Amaranthus protein liquid and Grain Production of Amaranthus sediment;

(2) make powder by after yellow meal worm microwave drying, put into the sodium hydroxide solution of 1.5mol/L, 70 DEG C of Water Unders bathe reaction 6 hours, and centrifugation 15 minutes, obtains yellow mealworm protein liquid and sediment;

(3) the Grain Production of Amaranthus protein liquid of step 1, the yellow mealworm protein liquid of step 2, SDPP, Bacillus natto are mixed, regulate PH to be 7.2,38 DEG C of condition bottom fermentations 32 hours, obtain protein peptides liquid;

(4) leaf of GAIGUO is added suitable quantity of water and grind to form juice, then stir into pureed after mixing with mussel meat, squeeze into press filtration in filter press, the press filtration rate of rise is 0.1Mpa/30 minute, obtains filtrate and filter residue;

(5) the yellow meal worm sediment of the Grain Production of Amaranthus sediment of step 1, step 2, the filter residue of step 4, peanut press pulp, saccharomycete are mixed, sealing, normal temperature bottom fermentation 20-24 hour, dry, granulation, obtains hybrid particles;

(6) Swine plasma, cheese are mixed, heating is stirred to cheese and melts completely, then mixes with the filtrate of the protein peptides liquid of step 3, step 4, edible oil and other residual components, is more evenly sprayed on the hybrid particles of step 5, dries.