CN110785427A - 一种长链多肽的纯化方法 - Google Patents
一种长链多肽的纯化方法 Download PDFInfo
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- CN110785427A CN110785427A CN201880000485.0A CN201880000485A CN110785427A CN 110785427 A CN110785427 A CN 110785427A CN 201880000485 A CN201880000485 A CN 201880000485A CN 110785427 A CN110785427 A CN 110785427A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Cardiology (AREA)
- Peptides Or Proteins (AREA)
Abstract
本发明提供了一种长链多肽的纯化方法,其包括步骤1),即纯化步骤:采用两根色谱柱串联进行粗品的分离,其中,上游色谱柱中填料的粒径比下游色谱柱填料粒径大;可选地,还包括作为转盐的步骤2):采用步骤1)中的上游色谱柱进行转盐,将步骤1)得到的目标峰产物进行上样;以95%‑85%的A2和5%‑15%的B冲洗15‑30min进行脱盐,其中,A2相为体积比0.05%‑0.2%的醋酸水溶液,B相为有机相乙腈,检测波长为230nm。
Description
PCT国内申请,说明书已公开。
Claims (9)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2018/089034 WO2019227342A1 (zh) | 2018-05-30 | 2018-05-30 | 一种长链多肽的纯化方法 |
Publications (2)
Publication Number | Publication Date |
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CN110785427A true CN110785427A (zh) | 2020-02-11 |
CN110785427B CN110785427B (zh) | 2023-09-05 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201880000485.0A Active CN110785427B (zh) | 2018-05-30 | 2018-05-30 | 一种长链多肽的纯化方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US11312744B2 (zh) |
EP (1) | EP3805246A4 (zh) |
CN (1) | CN110785427B (zh) |
WO (1) | WO2019227342A1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113683663A (zh) * | 2021-09-16 | 2021-11-23 | 杭州信海医药科技有限公司 | 一种机体保护多肽粗品的纯化方法 |
CN114478750A (zh) * | 2021-12-28 | 2022-05-13 | 深圳翰宇药业股份有限公司 | 一种特立帕肽的纯化方法 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113049690B (zh) * | 2019-12-27 | 2022-07-22 | 翰宇药业(武汉)有限公司 | 一种多肽脱盐的方法 |
CN113624898B (zh) * | 2021-08-23 | 2023-08-25 | 成都诺和晟泰生物科技有限公司 | 一种手性镇痛类多肽药物的纯化方法 |
US11806387B1 (en) | 2022-05-20 | 2023-11-07 | Radius Health, Inc. | Process of making abaloparatide |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2332247C1 (ru) * | 2007-06-01 | 2008-08-27 | Закрытое акционерное общество "БиоХимМак СТ" | Способ очистки иммуноглобулина (варианты) |
CN104045557A (zh) * | 2014-06-25 | 2014-09-17 | 上海华震科技有限公司 | 一种高纯度普伐他汀钠纯化工艺 |
CN105949284A (zh) * | 2016-05-26 | 2016-09-21 | 吉尔生化(上海)有限公司 | 一种纯化西那普肽的方法 |
US20180128720A1 (en) * | 2014-11-27 | 2018-05-10 | Hunan Demeter Instruments Co. Ltd. | Liquid phase chromatograph |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0763061B1 (de) * | 1994-06-02 | 2002-10-30 | Forssmann, Wolf-Georg, Prof. Dr. med. | Verfahren zur herstellung von cardiodilatin-fragmenten, hochgereinigte cardiodilatin-fragmente und zwischenprodukte zu deren herstellung |
CN104371018A (zh) * | 2014-01-22 | 2015-02-25 | 江苏汉邦科技有限公司 | 一种乌拉立肽的制备方法 |
US10690635B2 (en) * | 2016-03-23 | 2020-06-23 | Bachem Holding Ag | Purification of glucagon-like peptide 1 analogs |
CN106519009B (zh) * | 2016-10-26 | 2019-08-27 | 杭州固拓生物科技有限公司 | 一种乌拉立肽的制备方法 |
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2018
- 2018-05-30 CN CN201880000485.0A patent/CN110785427B/zh active Active
- 2018-05-30 US US15/733,862 patent/US11312744B2/en active Active
- 2018-05-30 WO PCT/CN2018/089034 patent/WO2019227342A1/zh unknown
- 2018-05-30 EP EP18921212.9A patent/EP3805246A4/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2332247C1 (ru) * | 2007-06-01 | 2008-08-27 | Закрытое акционерное общество "БиоХимМак СТ" | Способ очистки иммуноглобулина (варианты) |
CN104045557A (zh) * | 2014-06-25 | 2014-09-17 | 上海华震科技有限公司 | 一种高纯度普伐他汀钠纯化工艺 |
US20180128720A1 (en) * | 2014-11-27 | 2018-05-10 | Hunan Demeter Instruments Co. Ltd. | Liquid phase chromatograph |
CN105949284A (zh) * | 2016-05-26 | 2016-09-21 | 吉尔生化(上海)有限公司 | 一种纯化西那普肽的方法 |
Non-Patent Citations (2)
Title |
---|
李似姣: "《现代色谱分析》", 30 June 2014, 国防工业出版社 * |
覃玲莉等: "制备高效液相色谱法分离纯化乌拉立肽的研究", 《现代药物与临床》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113683663A (zh) * | 2021-09-16 | 2021-11-23 | 杭州信海医药科技有限公司 | 一种机体保护多肽粗品的纯化方法 |
CN114478750A (zh) * | 2021-12-28 | 2022-05-13 | 深圳翰宇药业股份有限公司 | 一种特立帕肽的纯化方法 |
CN114478750B (zh) * | 2021-12-28 | 2024-04-02 | 深圳翰宇药业股份有限公司 | 一种特立帕肽的纯化方法 |
Also Published As
Publication number | Publication date |
---|---|
CN110785427B (zh) | 2023-09-05 |
US11312744B2 (en) | 2022-04-26 |
WO2019227342A1 (zh) | 2019-12-05 |
EP3805246A1 (en) | 2021-04-14 |
US20210230216A1 (en) | 2021-07-29 |
EP3805246A4 (en) | 2022-03-23 |
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