CN110785427A - 一种长链多肽的纯化方法 - Google Patents

一种长链多肽的纯化方法 Download PDF

Info

Publication number
CN110785427A
CN110785427A CN201880000485.0A CN201880000485A CN110785427A CN 110785427 A CN110785427 A CN 110785427A CN 201880000485 A CN201880000485 A CN 201880000485A CN 110785427 A CN110785427 A CN 110785427A
Authority
CN
China
Prior art keywords
phase
chromatographic column
purification
column
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201880000485.0A
Other languages
English (en)
Other versions
CN110785427B (zh
Inventor
尹传龙
宓鹏程
陶安进
袁建成
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybio Pharmaceutical Co Ltd
Original Assignee
Hybio Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybio Pharmaceutical Co Ltd filed Critical Hybio Pharmaceutical Co Ltd
Publication of CN110785427A publication Critical patent/CN110785427A/zh
Application granted granted Critical
Publication of CN110785427B publication Critical patent/CN110785427B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Cardiology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本发明提供了一种长链多肽的纯化方法，其包括步骤1)，即纯化步骤：采用两根色谱柱串联进行粗品的分离，其中，上游色谱柱中填料的粒径比下游色谱柱填料粒径大；可选地，还包括作为转盐的步骤2)：采用步骤1)中的上游色谱柱进行转盐，将步骤1)得到的目标峰产物进行上样；以95％‑85％的A2和5％‑15％的B冲洗15‑30min进行脱盐，其中，A2相为体积比0.05％‑0.2％的醋酸水溶液，B相为有机相乙腈，检测波长为230nm。

Description

PCT国内申请，说明书已公开。

Claims (9)

  1. PCT国内申请，权利要求书已公开。
CN201880000485.0A 2018-05-30 2018-05-30 一种长链多肽的纯化方法 Active CN110785427B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/089034 WO2019227342A1 (zh) 2018-05-30 2018-05-30 一种长链多肽的纯化方法

Publications (2)

Publication Number Publication Date
CN110785427A true CN110785427A (zh) 2020-02-11
CN110785427B CN110785427B (zh) 2023-09-05

Family

ID=68697783

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880000485.0A Active CN110785427B (zh) 2018-05-30 2018-05-30 一种长链多肽的纯化方法

Country Status (4)

Country Link
US (1) US11312744B2 (zh)
EP (1) EP3805246A4 (zh)
CN (1) CN110785427B (zh)
WO (1) WO2019227342A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113683663A (zh) * 2021-09-16 2021-11-23 杭州信海医药科技有限公司 一种机体保护多肽粗品的纯化方法
CN114478750A (zh) * 2021-12-28 2022-05-13 深圳翰宇药业股份有限公司 一种特立帕肽的纯化方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113049690B (zh) * 2019-12-27 2022-07-22 翰宇药业（武汉）有限公司 一种多肽脱盐的方法
CN113624898B (zh) * 2021-08-23 2023-08-25 成都诺和晟泰生物科技有限公司 一种手性镇痛类多肽药物的纯化方法
US11806387B1 (en) 2022-05-20 2023-11-07 Radius Health, Inc. Process of making abaloparatide

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2332247C1 (ru) * 2007-06-01 2008-08-27 Закрытое акционерное общество "БиоХимМак СТ" Способ очистки иммуноглобулина (варианты)
CN104045557A (zh) * 2014-06-25 2014-09-17 上海华震科技有限公司 一种高纯度普伐他汀钠纯化工艺
CN105949284A (zh) * 2016-05-26 2016-09-21 吉尔生化（上海）有限公司 一种纯化西那普肽的方法
US20180128720A1 (en) * 2014-11-27 2018-05-10 Hunan Demeter Instruments Co. Ltd. Liquid phase chromatograph

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0763061B1 (de) * 1994-06-02 2002-10-30 Forssmann, Wolf-Georg, Prof. Dr. med. Verfahren zur herstellung von cardiodilatin-fragmenten, hochgereinigte cardiodilatin-fragmente und zwischenprodukte zu deren herstellung
CN104371018A (zh) * 2014-01-22 2015-02-25 江苏汉邦科技有限公司 一种乌拉立肽的制备方法
US10690635B2 (en) * 2016-03-23 2020-06-23 Bachem Holding Ag Purification of glucagon-like peptide 1 analogs
CN106519009B (zh) * 2016-10-26 2019-08-27 杭州固拓生物科技有限公司 一种乌拉立肽的制备方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2332247C1 (ru) * 2007-06-01 2008-08-27 Закрытое акционерное общество "БиоХимМак СТ" Способ очистки иммуноглобулина (варианты)
CN104045557A (zh) * 2014-06-25 2014-09-17 上海华震科技有限公司 一种高纯度普伐他汀钠纯化工艺
US20180128720A1 (en) * 2014-11-27 2018-05-10 Hunan Demeter Instruments Co. Ltd. Liquid phase chromatograph
CN105949284A (zh) * 2016-05-26 2016-09-21 吉尔生化（上海）有限公司 一种纯化西那普肽的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
李似姣: "《现代色谱分析》", 30 June 2014, 国防工业出版社 *
覃玲莉等: "制备高效液相色谱法分离纯化乌拉立肽的研究", 《现代药物与临床》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113683663A (zh) * 2021-09-16 2021-11-23 杭州信海医药科技有限公司 一种机体保护多肽粗品的纯化方法
CN114478750A (zh) * 2021-12-28 2022-05-13 深圳翰宇药业股份有限公司 一种特立帕肽的纯化方法
CN114478750B (zh) * 2021-12-28 2024-04-02 深圳翰宇药业股份有限公司 一种特立帕肽的纯化方法

Also Published As

Publication number Publication date
CN110785427B (zh) 2023-09-05
US11312744B2 (en) 2022-04-26
WO2019227342A1 (zh) 2019-12-05
EP3805246A1 (en) 2021-04-14
US20210230216A1 (en) 2021-07-29
EP3805246A4 (en) 2022-03-23

Similar Documents

Publication Publication Date Title
CN110785427A (zh) 一种长链多肽的纯化方法
EP2813514B1 (en) Method for purifying solid-phase synthetic crude liraglutide
CN110540587B (zh) 一种有效提高合成肽纯化收率的色谱方法
CN112661815B (zh) 一种Tirzepatide的纯化方法
CN105777872A (zh) 一种萨摩鲁肽的纯化方法
CN102212091A (zh) 一种高纯度栀子苷及其制剂的制备及临床应用
CN102993293A (zh) 一种特立帕肽醋酸盐的纯化方法
CN102219849B (zh) 一种大规模分离纯化艾塞那肽的方法
CN109503705A (zh) 一种利拉鲁肽的分离纯化方法
JP2019515664A (ja) 亜臨界水脱着技術によるモグロシドｖの分離・精製方法
CN101555279A (zh) 一种高纯度尿促***及其制备方法
CN111057142A (zh) 一种特立帕肽的纯化方法
CN113024658B (zh) 一种纯化利拉鲁肽的方法
Green [73] Purification of avidin
CN112175068B (zh) 一种司美格鲁肽的纯化方法
CN109053863A (zh) 一种低成本制备高纯度利那洛肽的方法
CN111269309B (zh) 一种glp-1类似多肽的纯化方法
CN109748948B (zh) 一种棕榈酰四肽-7的纯化方法
WO2020133993A1 (zh) 多肽的纯化方法
CN109406685B (zh) 一种分离卡非佐米和其异构体的高效液相色谱方法
CN105949284A (zh) 一种纯化西那普肽的方法
CN106928316A (zh) 一种纯化氧化含二硫键多肽的方法
CN115006419A (zh) 拟人参皂苷f11在制备治疗心力衰竭药物中的应用、治疗心力衰竭的药物及制备方法
CN113121675B (zh) 一种glp-1类似物的转盐方法
CN112500473A (zh) 一种醋酸艾塞那肽的分离纯化方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant