CN110301381A - A kind of artificial fecundation method of diplopore fish - Google Patents
A kind of artificial fecundation method of diplopore fish Download PDFInfo
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- CN110301381A CN110301381A CN201910769252.3A CN201910769252A CN110301381A CN 110301381 A CN110301381 A CN 110301381A CN 201910769252 A CN201910769252 A CN 201910769252A CN 110301381 A CN110301381 A CN 110301381A
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- diplopore
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- raun
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000012447 hatching Effects 0.000 claims abstract description 19
- 230000004720 fertilization Effects 0.000 claims abstract description 9
- 230000000384 rearing effect Effects 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims description 50
- 239000007924 injection Substances 0.000 claims description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- FGXWKSZFVQUSTL-UHFFFAOYSA-N domperidone Chemical compound C12=CC=CC=C2NC(=O)N1CCCN(CC1)CCC1N1C2=CC=C(Cl)C=C2NC1=O FGXWKSZFVQUSTL-UHFFFAOYSA-N 0.000 claims description 17
- 229960001253 domperidone Drugs 0.000 claims description 17
- 210000001015 abdomen Anatomy 0.000 claims description 13
- 235000013601 eggs Nutrition 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 12
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 10
- 230000032696 parturition Effects 0.000 claims description 6
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 5
- 239000004472 Lysine Substances 0.000 claims description 5
- 230000002051 biphasic effect Effects 0.000 claims description 5
- 230000037396 body weight Effects 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 239000003818 cinder Substances 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 230000009027 insemination Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 230000001360 synchronised effect Effects 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 235000013305 food Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241001085205 Prenanthella exigua Species 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of artificial fecundation method of diplopore fish, include the following steps: 1) parent population seed selection；2) parent fish rearing；3) artificial propagation；4) hatch.Diplopore fish is cultivated using the artificial fecundation method of science, fertilization, hatching rate are high, solve because the wild diplopore fish of economic aim is because of overfishing, and quantity falls sharply problem, explore the artificial propagation for realizing diplopore fish.
Description
Technical field
The invention belongs to technical field of fish culture, and in particular to a kind of artificial fecundation method of diplopore fish.
Background technique
Diplopore fish is Yunnan endemic species, is detected in Menhai, the Menla in Lancang River downstream.The oral area of diplopore fish forms sucker
Shape, so as to being adsorbed on the surfaces such as stone, the algae of shovel food block faces.Because adapting to torrent life, it is sucker that open height, which is evolved, is
The rare example that biology is adapted in environment has certain scientific research value on taxonomy of fishes and comparative anatomy,
It is all precious sample in teaching and scientific research, fisherman mostly captures in spawning season along the river in addition, therefore quantity falls sharply, endangered.
Because of diplopore fish reproductive characteristic, it would be highly desirable to invent a kind of artificial propagation techniques scheme of diplopore fish to solve above-mentioned technology
Problem.
Summary of the invention
The present invention provides a kind of technical method by artificial technology means ectogenesis diplopore fish.
Specific technical solution is: a kind of artificial fecundation method method of diplopore fish includes the following steps:
1) parent population is chosen seeds: parental source is fished in Lancang River field, is cultivated through domestication selection；
2) parent fish rearing:
A builds culture pond: bottom of pond is flat, and mud is less, area 0.07h ㎡ ~ 0.13h ㎡, depth of water 1.2m ~ 1.5m；
B male and female are with when stocking rate: male and female proportion is 1:1.5, and breeding density is 4 ~ 6 tails/㎡；
C feed with feed: launch artifical compound feed, day bait throwing in material be fish body weight 1% ~ 3%；
3) artificial propagation:
The selection of a parent population and combo: more than age, the long 150mm ~ 200mm of body, abdomen expands softness to raun 3；More than age, body is long for milter 2
100mm ~ 150mm is light that abdomen is pressed to have sperm outflow；Male and female proportion are as follows: 1:1；
The injection of b ocyodinic: injection LRH-A2, injection volume carry out second for raun after 0.5 μ g/kg, 6h -8h to raun for the first time
The ocyodinic of penetrating of secondary injection, injection is LRH-A2+ domperidone, and it be 5 μ g/kg+domperidone is 5mg/ that injection volume, which is LRH-A2,
kg；Milter injection volume is the half of raun injection volume, one injection synchronous with raun biphasic injection；
C artificial insemination: the parent population after hastening parturition carries out adopting ovum, semen collection when heat climax will lay eggs, and is containing mature essence, ovum
Fertilization is completed in device, and fertilized eggs are moved into hatchery and carry out Lotic hatching；
4) hatch.
Parental source is fished in Lancang River field, is cultivated through domestication selection.It obtains in group and selects from wild type
The speed of growth is with the obvious advantage, carries out cultivation parent population, uses as parent population.Culture pond has gone out area, depth of water requirement, and aquaculture pond is because clear
It is whole to meet national SC/T1008-2002 standard, while culture pond Ying Yu has abundant water resources, water quality is pure and fresh, and note row is convenient, and environment is relatively pacified
Jing Chu.
Diplopore fish it can be served after domestication artifical compound feed, parent population are raised based on artifical compound feed.
Further, it can allow in culture pond in the step 2 parent fish rearing and put worry feeding fish in a suitable place to breed.
Further, artifical compound feed ingredient is protein 40%, fatty 12%, crude fibre 6%, cinder in the step 2
Powder 18%, lysine 2%.
Further, the step 3) artificial propagation time is annual 5 ~ June.
When river rises annual June in rainy season ~ July in the Lancang River, diplopore fish lays eggs in streams.Pass through the parent after domestication
Fish, in place, reinforced cultivating, bait is sufficient for management measure, and parent population May ~ June, gonad development was good, i.e. raun abdomen in shape
Expand, lower abdomen is soft, cloacal aperture is ruddy, may be selected as promoting the sexual maturity and use fish；Milter has the milky sperm to scatter when slighter compress abdomen
Outflow, can select.
Further, the step 4) hatching is hatched using ring-like gutter channel.
Diplopore fish fertilized egg is buoyant egg, therefore ring-like gutter channel row is preferably used to hatch,
Further, the water temperature of the step 4) hatching is 24 DEG C ~ 26 DEG C.
Beneficial effects of the present invention: diplopore fish is cultivated using the artificial fecundation method of science, fertilization, hatching rate are high, solve
Because economic aim wild diplopore fish is because of overfishing, quantity falls sharply problem, explores the artificial propagation for realizing diplopore fish.
Specific embodiment
In order to which technical problem solved by the invention, technical solution is more clearly understood, with reference to embodiments, to this
Invention is further described.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, it is not used to
Limit the present invention.
Embodiment 1:
1) parent population is chosen seeds: parental source is fished in Lancang River field, is cultivated through domestication selection；
2) parent fish rearing:
A builds culture pond: bottom of pond is flat, and mud is less, area 0.07h ㎡, depth of water 1.2m；
B male and female are with when stocking rate: male and female proportion is 1:1.5, and breeding density is 4 tails/㎡, can allow in culture pond and puts worry food in a suitable place to breed
Property fish；
C feed with feed: launch artifical compound feed, day bait throwing in material be fish body weight 1%, artifical compound feed ingredient be albumen
Matter 40%, fatty 12%, crude fibre 6%, cinder powder 18%, lysine 2%；
3) artificial propagation, artificial propagation time are annual May:
The selection of a parent population and combo: more than age, the long 150mm of body, abdomen expands softness to raun 3；Milter 2 is more than age, the long 100mm of body,
Light pressure abdomen has sperm outflow；Male and female proportion are as follows: 1:1；
The injection of b ocyodinic: injection LRH-A2, injection volume carry out second for raun after 0.5 μ g/kg, 6h and infuse raun for the first time
It penetrates, the ocyodinic of penetrating of injection is LRH-A2+ domperidone (DOM), and injection volume is that LRH-A2 is 5 μ g/kg+domperidone (DOM)
For 5mg/kg；Milter injection volume is the half of raun injection volume, one injection synchronous with raun biphasic injection；Second of injection volume
How many pairs of rates of fertilization tool have a significant impact, such as table 1.
1: second injection volume contrast table of table；
Through inventor's test of many times, the results showed that it be 5 μ g+DOM is 5mg fertilization rate reached to 90% that ocyodinic injection volume, which is LRH-A2,
More than.
The Effect time of diplopore fish artificial induced spawning is calculated with second of ocyodinic time of hastening parturition, Effect time and water temperature, parent
Maturity, weather conditions of fish etc. have direct relation, and principal element is water temperature, as shown in table 2.
Table 2: Effect time；
C artificial insemination: the parent population after hastening parturition carries out adopting ovum, semen collection when heat climax will lay eggs, and is containing mature essence, ovum
Fertilization is completed in device, and fertilized eggs are moved into hatchery and carry out Lotic hatching；
4) hatch: being hatched using ring-like gutter channel, the water temperature of hatching is 24 DEG C, and brooding time, hatching rate are by Water Temperature
It is larger, as shown in table 3.
Table 3: brooding time
It is best in 24 DEG C ~ 26 DEG C hatching rates during this by Experimental Comparison water temperature, it can reach 80% or more.
Embodiment 2:
1) parent population is chosen seeds: parental source is fished in Lancang River field, is cultivated through domestication selection；
2) parent fish rearing:
A builds culture pond: bottom of pond is flat, and mud is less, area 0.10h ㎡, depth of water 1.4m；
B male and female are with when stocking rate: male and female proportion is 1:1.5, and breeding density is 5 tails/㎡, can allow in culture pond and puts worry food in a suitable place to breed
Property fish；
C feed with feed: launch artifical compound feed, day bait throwing in material be fish body weight 2%, artifical compound feed ingredient be albumen
Matter 40%, fatty 12%, crude fibre 6%, cinder powder 18%, lysine 2%；
3) artificial propagation, artificial propagation time are annual May:
The selection of a parent population and combo: more than age, the long 180mm of body, abdomen expands softness to raun 3；Milter 2 is more than age, the long 120mm of body,
Light pressure abdomen has sperm outflow；Male and female proportion are as follows: 1:1；
The injection of b ocyodinic: injection LRH-A2, injection volume carry out second for raun after 0.5 μ g/kg, 7h and infuse raun for the first time
It penetrates, the ocyodinic of penetrating of injection is LRH-A2+ domperidone (DOM), and injection volume is that LRH-A2 is 5 μ g/kg+domperidone (DOM)
For 5mg/kg；Milter injection volume is the half of raun injection volume, one injection synchronous with raun biphasic injection；
C artificial insemination: the parent population after hastening parturition carries out adopting ovum, semen collection when heat climax will lay eggs, and is containing mature essence, ovum
Fertilization is completed in device, and fertilized eggs are moved into hatchery and carry out Lotic hatching；
4) hatch: being hatched using ring-like gutter channel, the water temperature of hatching is 25 DEG C, and brooding time, hatching rate are by Water Temperature
It is larger.
It is best in 24 DEG C ~ 26 DEG C hatching rates during this by Experimental Comparison water temperature, it can reach 80% or more.
Embodiment 3:
1) parent population is chosen seeds: parental source is fished in Lancang River field, is cultivated through domestication selection；
2) parent fish rearing:
A builds culture pond: bottom of pond is flat, and mud is less, area 0.13h ㎡, depth of water 1.5m；
B male and female are with when stocking rate: male and female proportion is 1:1.5, and breeding density is 6 tails/㎡, can allow in culture pond and puts worry food in a suitable place to breed
Property fish；
C feed with feed: launch artifical compound feed, day bait throwing in material be fish body weight 3%, artifical compound feed ingredient be albumen
Matter 40%, fatty 12%, crude fibre 6%, cinder powder 18%, lysine 2%；
3) artificial propagation, artificial propagation time are annual June:
The selection of a parent population and combo: more than age, the long 200mm of body, abdomen expands softness to raun 3；Milter 2 is more than age, the long 150mm of body,
Light pressure abdomen has sperm outflow；Male and female proportion are as follows: 1:1；
The injection of b ocyodinic: injection LRH-A2, injection volume carry out second for raun after 0.5 μ g/kg, 7h and infuse raun for the first time
It penetrates, the ocyodinic of penetrating of injection is LRH-A2+ domperidone (DOM), and injection volume is that LRH-A2 is 5 μ g/kg+domperidone (DOM)
For 5mg/kg；Milter injection volume is the half of raun injection volume, one injection synchronous with raun biphasic injection；
C artificial insemination: the parent population after hastening parturition carries out adopting ovum, semen collection when heat climax will lay eggs, and is containing mature essence, ovum
Fertilization is completed in device, and fertilized eggs are moved into hatchery and carry out Lotic hatching；
4) hatch: being hatched using ring-like gutter channel, the water temperature of hatching is 26 DEG C, and brooding time, hatching rate are by Water Temperature
It is larger.
It is best in 24 DEG C ~ 26 DEG C hatching rates during this by Experimental Comparison water temperature, it can reach 80% or more.
The present invention is described in detail above by specific and preferred embodiment, but those skilled in the art should be bright
White, the present invention is not limit to that embodiments describe above, all within the spirits and principles of the present invention, made any modification,
Equivalent replacement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of artificial fecundation method method of diplopore fish, characterized by the following steps:
1) parent population is chosen seeds: parental source is fished in Lancang River field, is cultivated through domestication selection；
2) parent fish rearing:
A builds culture pond: bottom of pond is flat, and mud is less, area 0.07h ㎡ ~ 0.13h ㎡, depth of water 1.2m ~ 1.5m；
B male and female are with when stocking rate: male and female proportion is 1:1.5, and breeding density is 4 ~ 6 tails/㎡；
C feed with feed: launch artifical compound feed, day bait throwing in material be fish body weight 1% ~ 3%；
3) artificial propagation:
The selection of a parent population and combo: more than age, the long 150mm ~ 200mm of body, abdomen expands softness to raun 3；More than age, body is long for milter 2
100mm ~ 150mm is light that abdomen is pressed to have sperm outflow；Male and female proportion are as follows: 1:1；
The injection of b ocyodinic: injection LRH-A2, injection volume carry out second for raun after 0.5 μ g/kg, 6h -8h to raun for the first time
The ocyodinic of penetrating of secondary injection, injection is LRH-A2+ domperidone, and it be 5 μ g/kg+domperidone is 5mg/ that injection volume, which is LRH-A2,
kg；Milter injection volume is the half of raun injection volume, one injection synchronous with raun biphasic injection；
C artificial insemination: the parent population after hastening parturition carries out adopting ovum, semen collection when heat climax will lay eggs, and is containing mature essence, ovum
Fertilization is completed in device, and fertilized eggs are moved into hatchery and carry out Lotic hatching；
4) hatch.
2. a kind of artificial fecundation method method of diplopore fish according to claim 1, it is characterised in that: the step 2 parent
It can allow in culture pond in fish cultivation and put worry feeding fish in a suitable place to breed.
3. a kind of artificial fecundation method method of diplopore fish according to claim 1, it is characterised in that: in the step 2
Artifical compound feed ingredient is protein 40%, fatty 12%, crude fibre 6%, cinder powder 18%, lysine 2%.
4. a kind of artificial fecundation method method of diplopore fish according to claim 1, it is characterised in that: the step 3) people
Work repoductive time is annual 5 ~ June.
5. a kind of artificial fecundation method method of diplopore fish according to claim 1, it is characterised in that: the step 4) is incubated
Change and is hatched using ring-like gutter channel.
6. a kind of artificial fecundation method method of diplopore fish according to claim 1-5, it is characterised in that: described
The water temperature of step 4) hatching is 24 DEG C ~ 26 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110741984A (en) * | 2019-11-19 | 2020-02-04 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of meinan silurus meridionalis |
| CN110741986A (en) * | 2019-11-19 | 2020-02-04 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of silurus hamiltonii |
| CN110741985A (en) * | 2019-11-19 | 2020-02-04 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of sinocyclocheilus grahami |
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| US20100035327A1 (en) * | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
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Application publication date: 20191008 |