CN110721244A - Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof - Google Patents
Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof Download PDFInfo
- Publication number
- CN110721244A CN110721244A CN201911161942.7A CN201911161942A CN110721244A CN 110721244 A CN110721244 A CN 110721244A CN 201911161942 A CN201911161942 A CN 201911161942A CN 110721244 A CN110721244 A CN 110721244A
- Authority
- CN
- China
- Prior art keywords
- extract
- callicarpa nudiflora
- organic solvent
- preparing
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Rheumatology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Pain & Pain Management (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Endocrinology (AREA)
- Reproductive Health (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to the field of traditional Chinese medicine extraction, and in particular relates to a callicarpa nudiflora extract, a preparation method and an application thereof in resisting cervicitis.
Description
Technical Field
The invention relates to the field of traditional Chinese medicine extraction, in particular to a preparation method of a callicarpa nudiflora extract and application thereof in cervicitis resistance.
Background
The world health organization's survey of chinese women showed that: about 41 percent of women in childbearing age in China have gynecological inflammatory diseases with different degrees, and the incidence rate of married women is higher to 70 percent. According to statistics, the cervicitis accounts for about 40-50% of the total number of outpatients in obstetrics and gynecology department, and the chronic cervicitis accounts for 10-25% of outpatients, so the development of the anti-cervicitis preparation with definite curative effect is an urgent requirement of most patients.
Callicarpa nudiflora is dry leaf of Callicarpa of Verbenaceae, and is from Verbenaceae, and recorded in Chinese medicinal dictionary, Callicarpa davidii is Callicarpa pallidiflora and Callicarpa chinensis of Callicarpa, and has effects of removing toxic substance, treating sore, astringing, stopping bleeding, dispelling pathogenic wind, and eliminating dampness, and can be used for treating scald, burn, traumatic hemorrhage, etc.
The existing research shows that the callicarpa nudiflora extract has the pharmacological activities of promoting hemostasis, inhibiting thrombosis, inhibiting bacteria and cell toxicity, resisting inflammation, enhancing immunity and the like.
The nakedflower beautyberry bolt, the nakedflower beautyberry tablet and the nakedflower beautyberry capsule which take the nakedflower beautyberry as the main medicine are widely applied to the treatment of gynecological diseases and skin diseases and have obvious effect. Although the efficacy of the callicarpa nudiflora as a compound medicine is exact, the research on the aspect of treating cervicitis by using a single medicine of the callicarpa nudiflora does not exist, so that the invention takes the callicarpa nudiflora as a research object to research the anti-inflammation, hemostasis and the effect of the callicarpa nudiflora on a phenol cervicitis rat model, and discloses the action mechanism of the callicarpa nudiflora for resisting cervicitis.
Disclosure of Invention
The invention provides an n-butanol extract in callicarpa nudiflora and a preparation method thereof, researches the application of single medicine in anti-cervicitis, and discloses the action mechanism of callicarpa nudiflora in anti-cervicitis.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the invention aims to provide a callicarpa nudiflora extract, which is a callicarpa nudiflora n-butanol extract, and is extracted from callicarpa nudiflora by n-butanol solution.
Another object of the present invention is to provide a method for preparing the callicarpa nudiflora extract, comprising the steps of:
(1) pulverizing folium Callicarpae Formosanae, adding into tap bottle, adding 90% ethanol solution for the first time, soaking for 24 hr, collecting extractive solution, and washing with 90% ethanol.
(2) Taking Callicarpa nudiflora leaf powder, preparing 90% ethanol solution for the first time, soaking for 24h, washing, and adding 90% ethanol to obtain Callicarpa nudiflora extract A.
(3) And (3) carrying out rotary evaporation on the callicarpa nudiflora extract A to obtain an extractum-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, sequentially carrying out fractional extraction on the dispersion liquid by using an organic solvent C, an organic solvent D and an organic solvent E, and concentrating an extraction liquid obtained in the last extraction to obtain an extract.
Preferably, the method comprises the following steps:
(1) crushing a certain amount of callicarpa nudiflora leaves, then adding the crushed callicarpa nudiflora leaves into a faucet bottle, firstly adding 4000-6000mL of 90% ethanol solution, soaking for 24h, collecting extract liquid, preparing 3000mL of 90% ethanol, and starting washing (the liquid level is required to be not lower than the medicine residues).
(2) Taking a certain amount of callicarpa nudiflora leaf powder, firstly preparing 5000mL of 90% ethanol, soaking for 24h, washing, and then sequentially adding 8000mL of 90% ethanol to obtain callicarpa nudiflora extract A.
(3) And (4) carrying out rotary evaporation on the callicarpa nudiflora extract B to obtain an extract-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, sequentially carrying out fractional extraction on the dispersion liquid by using an organic solvent C, an organic solvent D and an organic solvent E, and concentrating an extraction liquid obtained in the last extraction to obtain an extract.
Preferably, the organic solvent D is petroleum ether, the organic solvent E is ethyl acetate, and the organic solvent F is n-butanol.
Preferably, the callicarpa nudiflora extract B is subjected to rotary evaporation at 40-45 ℃, and the extractum-shaped object C has no alcohol smell.
The third purpose of the present invention is to study the anti-cervicitis application of the beautyberry extract, which is used for reducing tumor necrosis factor, prostaglandin and increasing interleukin.
Preferably, the callicarpa nudiflora extract is used for repairing cervicitis rat cervical tissue.
The n-butanol extract in the callicarpa nudiflora, the preparation method and the application thereof in resisting cervicitis provided by the invention have the following beneficial effects:
(1) the invention extracts the n-butyl alcohol part of the callicarpa nudiflora for the first time, and researches on the application of the n-butyl alcohol extract of the callicarpa nudiflora alone to cervicitis show that the n-butyl alcohol extract of the callicarpa nudiflora can obviously reduce TNF-alpha and PGE2And the IL-1 index is improved, the anti-cervicitis activity is stronger, and cervicitis pathological change tissues can be obviously repaired.
(2) The preparation method of the callicarpa nudiflora n-butanol extract is simple and easy to implement and low in cost.
Drawings
FIG. 1 is a flow chart of the pathological histological examination of female SD rats according to the present invention.
Fig. 2 is a pathological section of cervical tissue of a blank rat.
Fig. 3 is a pathological section of cervical tissue of a rat in a model group.
FIG. 4 is a pathological section of cervical tissue of rats in the n-butanol concentration group of Callicarpa nudiflora (group A1).
FIG. 5 is a pathological section of the cervical tissue of rats in the group of concentration of n-butanol in Callicarpa nudiflora (i.e., group A2).
FIG. 6 is a pathological section of cervical tissue of rats in the callicarpa nudiflora n-butanol high concentration group (i.e., group A3).
FIG. 7 is a pathological section of cervical tissue of rat in Fuyanjie group.
Fig. 8 is a pathological section of rat cervix with n-butanol.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments.
The apparatus and materials used in the present invention are as follows:
KQ-250DE type numerical control ultrasonic cleaner; PH07-9076A electric heating constant temperature air-blast drying oven (Shanghai sperm macro experimental equipment Co., Ltd.); SHB-IIIA circulating water type multipurpose vacuum pump; WB-200 great wall water bath and R-1002N great wall rotary evaporator (zheng zhou great wall science and trade, ltd.).
48 SD rats with female body weight of 180-220 g are provided by animal experiment center in Henan province, and have a certificate number (SCXK 2016-0001). The laboratory temperature is 21-25 ℃, the air is circulated, the relative humidity is 55% -60%, and the water is freely eaten and drunk after 12 hours of light-dark alternate illumination.
N-butanol: shanghai Ji to Biochemical technology, Inc., product number N15270, analytically pure;
phenol: shanghai Ji to Biochemical technology, Inc., product number P33740, analytically pure;
fuyanjie plant herbal antibacterial lotion: jiangxi Kenren and pharmaceutical Co., Ltd;
callicarpa nudiflora is a dry aerial part purchased from underwriting pharmaceutical sales Limited, Bozhou.
The first and the following examples are the preparation method of nakedflower beautyberry extract, which is an n-butanol fraction of nakedflower beautyberry.
Example 1: the preparation method of the callicarpa nudiflora extract comprises the following steps:
(1) pulverizing folium Callicarpae Formosanae, adding into a tap bottle, adding 90% ethanol solution for the first time, soaking for 24 hr, collecting extractive solution, preparing 3000mL 90% ethanol, and washing (liquid level is not lower than the residue).
(2) Taking Callicarpa nudiflora leaf powder, preparing 90% ethanol solution for the first time, soaking for 24h, washing, and adding 8000mL of 90% ethanol to obtain Callicarpa nudiflora extract A.
(3) And (3) carrying out rotary evaporation on the callicarpa nudiflora extract A to obtain an extractum-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, dividing the dispersion liquid into two parts with equal volumes, sequentially carrying out fractional extraction on each part by using petroleum ether, ethyl acetate and n-butyl alcohol to obtain a petroleum ether part extract, an ethyl acetate part extract and an n-butyl alcohol part extract respectively, extracting for 3 times by using each dispersion liquid respectively, collecting the n-butyl alcohol part extracts obtained by each extraction in a centralized manner, carrying out rotary evaporation at the temperature of 55-60 ℃, concentrating until the n-butyl alcohol smell does not exist to obtain an n-butyl alcohol extract of callicarpa nudiflora, containing the n-butyl alcohol extract by using a triangular flask, and storing the n-butyl.
Example 2: the preparation method of the callicarpa nudiflora extract comprises the following steps:
(1) 1081g of callicarpa nudiflora leaf is taken to be ground, then added into a faucet bottle, 4000mL of 90% ethanol solution in mass fraction is added for the first time, soaked for 24h, the extract is collected, 2500mL of 90% ethanol solution in mass fraction is prepared at the same time, and washing is started (the liquid level is required to be not lower than the medicine residue).
(2) Taking 1000g of beautyberry leaf powder, preparing 4000ml of 90% ethanol solution for the first time, soaking for 24h, and adding 7000ml of 90% ethanol after washing to obtain a beautyberry extract A.
(3) Concentrating the callicarpa nudiflora extract A at 40-45 ℃ by adopting a rotary evaporator to obtain an extractum-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, dividing the dispersion liquid into two parts with equal volumes, sequentially carrying out fractional extraction on each part by using petroleum ether, ethyl acetate and n-butyl alcohol to obtain a petroleum ether part extract, an ethyl acetate part extract and an n-butyl alcohol part extract respectively, extracting for 3 times by using each dispersion liquid respectively, collecting the n-butyl alcohol part extracts obtained by each extraction in a centralized manner, carrying out rotary evaporation at the temperature of 55-60 ℃, concentrating until the n-butyl alcohol smell does not exist to obtain an n-butyl alcohol extract of callicarpa nudiflora, containing the n-butyl alcohol extract by using a triangular flask, and storing the n-butyl.
Example 3: the preparation method of the callicarpa nudiflora extract comprises the following steps:
(1) pulverizing 1081g folium Callicarpae Formosanae, adding into a faucet bottle, adding 6000mL 90% ethanol solution for the first time, soaking for 24h, collecting extractive solution, preparing 3500mL 90% ethanol solution, and washing (liquid level is required to be not lower than the residue).
(2) Taking 1000g of callicarpa nudiflora leaf powder, preparing 6000ml of 90% ethanol solution for the first time, soaking for 24h, and continuously adding 9000ml of 90% ethanol after washing to obtain callicarpa nudiflora extract A.
(3) Concentrating the callicarpa nudiflora extract A at 40-45 ℃ by adopting a rotary evaporator to obtain an extractum-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, dividing the dispersion liquid into two parts with equal volumes, sequentially carrying out fractional extraction on each part by using petroleum ether, ethyl acetate and n-butyl alcohol to obtain a petroleum ether part extract, an ethyl acetate part extract and an n-butyl alcohol part extract respectively, extracting for 3 times by using each dispersion liquid respectively, collecting the n-butyl alcohol part extracts obtained by each extraction in a centralized manner, carrying out rotary evaporation at the temperature of 55-60 ℃, concentrating until the n-butyl alcohol smell does not exist to obtain an n-butyl alcohol extract of callicarpa nudiflora, containing the n-butyl alcohol extract by using a triangular flask, and storing the n-butyl.
Example 4: the preparation method of the callicarpa nudiflora extract comprises the following steps:
(1) 1081g of callicarpa nudiflora leaf is taken to be crushed, then added into a faucet bottle, 5000mL of ethanol solution with the mass fraction of 90% is added for the first time, soaked for 24 hours, extract liquid is collected, 3000mL of ethanol solution with the mass fraction of 90% is prepared at the same time, and washing is started (the liquid level is required to be not lower than the medicine residue).
(2) Taking 1000g of callicarpa nudiflora leaf powder, preparing 5000ml of 90% ethanol solution for the first time, soaking for 24h, washing, and then adding 8000ml of 90% ethanol to obtain callicarpa nudiflora extract A.
(3) Concentrating the callicarpa nudiflora extract A at 40-45 ℃ by adopting a rotary evaporator to obtain an extractum-shaped object B.
(4) Adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, dividing the dispersion liquid into two parts with equal volumes, sequentially carrying out fractional extraction on each part by using petroleum ether, ethyl acetate and n-butyl alcohol to obtain a petroleum ether part extract, an ethyl acetate part extract and an n-butyl alcohol part extract respectively, extracting for 3 times by using each dispersion liquid respectively, collecting the n-butyl alcohol part extracts obtained by each extraction in a centralized manner, carrying out rotary evaporation at the temperature of 55-60 ℃, concentrating until the n-butyl alcohol smell does not exist to obtain an n-butyl alcohol extract of callicarpa nudiflora, containing the n-butyl alcohol extract by using a triangular flask, and storing the n-butyl.
Application of n-butanol extract of callicarpa nudiflora in resisting cervicitis
1. Establishment of cervicitis model of rat
(1) Preparing phenol mucilage: 5g of phenol and 15g of Arabic gum powder are precisely weighed, mixed and added with distilled water to 20mL, namely phenol mucilage with the mass fraction of 20 percent.
(2) Injecting a stomach needle with a volume of l mL, inserting into the deep vagina of a rat at a position of about 0.9-1.1cm, injecting 0.2-0.3mL of 20% phenol gel in the solution (1), injecting 1 time per day, and continuously injecting for 7 days. During the molding process, the white secretion, red swelling and congestion of the vagina of the rat are observed.
2. Activity test of n-butanol extract of callicarpa nudiflora for resisting cervicitis of rats
(1) And (3) injection observation: cervicitis rats were divided into 6 groups:
drug group: the medicine group is obtained by vaginally administering the n-butyl alcohol extract of beautyberry, and is divided into A1, A2 and A3 groups according to the concentration of the n-butyl alcohol extract of beautyberry, wherein the concentration of the n-butyl alcohol extract of beautyberry is lower in the A1 group, the concentration of the n-butyl alcohol extract of beautyberry is medium in the A2 group, and the concentration of the n-butyl alcohol extract of beautyberry is higher in the A3 group;
model group: the model group to which the n-butanol extract of Callicarpa nudiflora was not administered;
the gynecological inflammation cleaning group comprises: the vagina is provided with the gynecological inflammation cleaning group of the herbal antibacterial lotion for the gynecological inflammation cleaning;
n-butanol group: n-butanol excipient for vaginal administration is n-butanol;
in addition, a blank group is also provided, wherein the blank group is a group of normal rats without cervicitis.
Drug group: respectively administering n-butanol extract of Callicarpa nudiflora at concentrations of 0.9998g/kg, 1.9748g/kg and 4.4g/kg to vagina of rats with cervicitis to obtain low concentration group (A1 group), medium concentration group (A2 group) and high concentration group (A3 group),
the gynecological inflammation cleaning group comprises: injecting 2.5ml of Fuyanjie plant herbal bacteriostatic lotion with the mass fraction of 3% into the vagina of a rat in the Fuyanjie group, continuously administering for 14 days, and observing various changes of the rat in the group.
N-butanol group: the rats of n-butanol group were injected with n-butanol 0.02g/kg (skin irritation amount of rats) vaginally, and the rats of the group were observed for each change by continuous administration for 14 d.
(2) Measurement indexes are as follows: the method comprises the steps of determining the contents of tumor necrosis factor (TNF-alpha), interleukin (IL-1) and prostaglandin (PGE2) in the plasma of each group of rats, killing animals the next day after the last administration of each group of rats, collecting blood of about 4ml from the heart, placing the blood in a sodium citrate anticoagulation tube, centrifuging at 3000rpm for 15min, separating serum, and determining each index by adopting an enzyme-linked reaction adsorption method.
(3) And (3) measuring results: statistical processing, all data processed using SPSS 13.0 statistical software, to
And (4) showing. The samples of each group are analyzed by adopting single-factor variance analysis, the homogeneity of the variances is detected by adopting LSD, and the difference is taken as P less than 0.05, so that the statistical significance is achieved.
TABLE 1 n-butanol extract of Callicarpa nudiflora on cervicitis rat TNF-alpha, IL-1, PGE2Influence of (2)
n＝6
*: model group vs. blank group, p<0.05；**: model group vs. blank group, p<0.01。
#: drug group vs blank group, p<0.05；##: drug group vs blank group, p<0.01。
△: compared with the Fuyanjie group, the medicine group is p<0.05；△△: compared with the Fuyanjie group, the medicine group is p<0.01。
Application of n-butanol extract of Callicarpa nudiflora to TNF-alpha, IL-1 and PGE of cervicitis rat2The effects of (A) and (B) can be seen in Table 1, TNF-alpha and PGE for the model set2Compared with blank group, the level is obviously increased and has significant difference (P)<0.01), IL-1 level decreased compared to blank, P<0.05, there is statistical difference, which shows that the establishment of the rat cervicitis model is successful.
TNF-alpha and PGE of three concentration groups of nakedflower beautyberry n-butanol extract2All had significant reduction (P) compared with the model group<0.05); IL-1 levels in the medium and high concentration groups (i.e., A2 and A3) of n-butanol extract of Callicarpa nudiflora were reduced compared to the model group, and P was found to be lower than that in the model group<0.05, has statistical difference, and shows that the n-butyl alcohol extract of the lithospermum nudiflorum has an anti-inflammatory effect. TNF-alpha, IL-1 and PGE compared with the model group in the Fuyanjie group2Slightly lower level, P<0.05, with statistical differences. TNF-alpha, IL-1, PGE, compared to n-butanol2Close to the horizontal, P>0.05, no statistical significance.
3. Influence of n-butanol extract of Callicarpa nudiflora on cervical tissue of cervicitis rat
(1) Pathological histological examination: the pathological histology examination process of female SD rat is shown in figure 1
(2) The research results are as follows: FIGS. 2-8 are the pathological section views of the cervical tissue of rats in each group,
as can be seen from FIG. 2, the tissue structure of the cervical mucosa of the rats in the blank group is clear and complete, the structure of the squamous columnar epithelial transition part is normal, and lymphocytes and eosinophils are sporadically scattered in the cervical mucosa and submucosal tissues.
As can be seen from FIG. 3, the epithelial tissues of the cervical mucosa of the rats in the model group are obviously degenerated and necrosed, partially exfoliated, and the glands are destroyed, so that a large amount of lymphocytes and neutrophil infiltration can be seen in the cervical mucosa and the interstitium, and interstitial fibroplasia and a large amount of inflammatory exudates can be seen in the uterine cavity.
Fig. 4 shows that the pathological changes of rats in the low concentration drug group are reduced, but are not obvious, a large amount of inflammatory cells such as eosinophils and lymphocyte infiltration are still remained from the mucous membrane to the stroma, the inflammatory exudate and necrotic tissues in the uterine cavity are obviously reduced, and part of the cervical mucous membrane is close to normal.
Fig. 5 shows that the pathological changes of the cervical tissues of rats in the medium concentration group of the drug group are obviously reduced compared with the model group, the epithelium of the cervical mucosa is not recovered to be normal, but no obvious squamous epithelial hyperplasia and degeneration necrosis are generated, a little inflammatory cell infiltration is occasionally seen from the mucosa to the interstitium, and no obvious inflammatory exudation is seen in the uterine cavity.
As can be seen in fig. 6, the cervical mucosal epithelium of rats in the high concentration group of the drug group had been substantially restored, some of the cervical mucosa had been nearly normal, a small amount of inflammatory cell infiltration was occasionally seen from mucosa to interstitium, and no inflammatory exudate was seen in the cavity.
Fig. 7 shows that the pathological changes of the cervical tissues of the rats in the Fuyanjie group are reduced compared with the cervical tissue of the model group, the epithelium of the cervical mucosa is not completely repaired, the part is close to normal, a small amount of inflammatory cell infiltration is occasionally seen from the mucosa to the stroma, and no inflammatory exudate and necrotic tissues are seen in the uterine cavity.
As can be seen in FIG. 8, the lesion of the cervical tissue of the rat with n-butanol group was reduced as compared to the model group.
Therefore, the n-butyl alcohol extract of the callicarpa nudiflora has obvious positive effect on repairing cervical tissues.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The callicarpa nudiflora extract is characterized in that: the extract is a nakedflower beautyberry n-butanol extract, and the nakedflower beautyberry n-butanol extract is extracted from nakedflower beautyberry by using n-butanol solution.
2. The method for preparing the callicarpa nudiflora extract as claimed in claim 1, wherein: the method comprises the following steps:
(1) crushing callicarpa nudiflora leaves, then adding the crushed callicarpa nudiflora leaves into a faucet bottle, firstly adding an ethanol solution with the mass fraction of 90%, soaking for 24 hours, collecting an extract, and washing with 90% ethanol at the same time;
(2) taking Callicarpa nudiflora leaf powder, preparing 90% ethanol solution for the first time, soaking for 24h, washing, and sequentially adding 90% ethanol to obtain Callicarpa nudiflora extract A;
(3) carrying out rotary evaporation on the callicarpa nudiflora extract A to obtain an extractum-shaped object B;
(4) adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, sequentially carrying out fractional extraction on the dispersion liquid by using an organic solvent C, an organic solvent D and an organic solvent E, and concentrating an extraction liquid obtained in the last extraction to obtain an extract.
3. The method for preparing the callicarpa nudiflora extract as claimed in claim 1, wherein: the method comprises the following steps:
(1) crushing a certain amount of callicarpa nudiflora leaves, then adding the crushed callicarpa nudiflora leaves into a faucet bottle, adding 4000-6000mL of 90% ethanol solution for the first time, soaking for 24 hours, collecting extract liquid, preparing 3000mL of 90% ethanol, and starting washing;
(2) taking a certain amount of callicarpa nudiflora leaf powder, preparing 5000mL of 90% ethanol for soaking for 24h for the first time, and adding 8000mL of 90% ethanol after washing to obtain callicarpa nudiflora extract A;
(3) carrying out rotary evaporation on the callicarpa nudiflora extract B to obtain an extract-shaped object B;
(4) adding ultrapure water into the extractum-shaped object B, carrying out ultrasonic oscillation to obtain a dispersion liquid, sequentially carrying out fractional extraction on the dispersion liquid by using an organic solvent C, an organic solvent D and an organic solvent E, and concentrating an extraction liquid obtained in the last extraction to obtain an extract.
4. The method for preparing the callicarpa nudiflora extract as claimed in claim 1, wherein: the organic solvent D is petroleum ether, the organic solvent E is ethyl acetate, and the organic solvent F is n-butanol.
5. The method for preparing the callicarpa nudiflora extract as claimed in claim 1, wherein: the callicarpa nudiflora extract B is subjected to rotary evaporation at 40-45 ℃, and the extractum-shaped object C has no alcohol smell.
6. The use of the beautyberry extract according to claim 1 for the treatment of cervicitis, which comprises: the Callicarpa nudiflora extract is used for reducing tumor necrosis factor, prostaglandin and increasing interleukin.
7. The use of the beautyberry extract according to claim 1 for the treatment of cervicitis, which comprises: the callicarpa nudiflora extract has the effect of repairing cervicitis rat cervical tissues.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911161942.7A CN110721244A (en) | 2019-11-25 | 2019-11-25 | Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911161942.7A CN110721244A (en) | 2019-11-25 | 2019-11-25 | Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110721244A true CN110721244A (en) | 2020-01-24 |
Family
ID=69224745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911161942.7A Pending CN110721244A (en) | 2019-11-25 | 2019-11-25 | Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110721244A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007210977A (en) * | 2006-02-13 | 2007-08-23 | Maruzen Pharmaceut Co Ltd | Anti-ageing agent, and slimming agent, and skin cosmetic |
| CN101890114A (en) * | 2009-05-23 | 2010-11-24 | 海南省南药黎药研究院 | Li nation medicament extract and composition with bacteriostatic, anti-inflammatory and hemostasis effects and preparation method thereof |
| CN106266262A (en) * | 2015-06-05 | 2017-01-04 | 九芝堂股份有限公司 | A kind of Callicarpa nudiflora extract with antiinflammatory action |
| CN108524684A (en) * | 2018-06-28 | 2018-09-14 | 黄河科技学院 | Effective fraction with anti-cervicitis effect in callicarpa nudiflora |
-
2019
- 2019-11-25 CN CN201911161942.7A patent/CN110721244A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007210977A (en) * | 2006-02-13 | 2007-08-23 | Maruzen Pharmaceut Co Ltd | Anti-ageing agent, and slimming agent, and skin cosmetic |
| CN101890114A (en) * | 2009-05-23 | 2010-11-24 | 海南省南药黎药研究院 | Li nation medicament extract and composition with bacteriostatic, anti-inflammatory and hemostasis effects and preparation method thereof |
| CN106266262A (en) * | 2015-06-05 | 2017-01-04 | 九芝堂股份有限公司 | A kind of Callicarpa nudiflora extract with antiinflammatory action |
| CN108524684A (en) * | 2018-06-28 | 2018-09-14 | 黄河科技学院 | Effective fraction with anti-cervicitis effect in callicarpa nudiflora |
Non-Patent Citations (4)
| Title |
|---|
| 潘争红 等: "裸花紫珠黄酮苷类化合物的分离与结构鉴定", 《中国实验方剂学杂志》 * |
| 王杰 等: "裸花紫珠正丁醇部位的化学成分研究", 《中国药学杂志》 * |
| 王秀芳 等: "木犀草苷对小鼠非酒精性脂肪肝病的作用研究", 《中华全科医学》 * |
| 蒋步云 等: "植物多酚作为动物饲料添加剂的研究进展", 《中国畜牧杂志》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Thakur et al. | Practices in wound healing studies of plants | |
| CN101530473A (en) | Nose-dredging spraying agent and preparation method thereof | |
| Zheng et al. | The anti-inflammatory and anti-nociceptive activities ofPatrinia villosa and its mechanism on the proinflammatory cytokines of rats with pelvic inflammation | |
| JP2021516696A (en) | Chinese herbal medicine composition, Chinese herbal medicine combination preparation and its preparation method and application | |
| CN105560331A (en) | Eczematous dermatitis prevention medicine, and preparation method and application thereof | |
| Liu et al. | Preparation and safety evaluation of Centella asiatica total glycosides nitric oxide gel and its therapeutic effect on diabetic cutaneous ulcers | |
| CN101062085B (en) | Condyloma acuminata treating medicine | |
| CN110721244A (en) | Callicarpa nudiflora extract, preparation method and anti-cervicitis application thereof | |
| WO2022028375A1 (en) | Therapeutic use of cell-free fat extract solution for pulmonary diseases | |
| CN106177900B (en) | A kind of gel of anti-human papilloma virus (anti-HPV) and preparation method thereof | |
| CN113712999A (en) | Application of Zhuang medicine xue Dang dry paste powder in preparation of anti-inflammatory analgesic medicine | |
| CN110917255B (en) | Preparation method and application of novel medicine for resisting endometritis | |
| CN113018365A (en) | Compound dragon's blood dental ulcer medicine composition and preparation method thereof | |
| Novianty et al. | Effect of allicin for re-epithelialization during healing in oral ulcer model | |
| CN102349935A (en) | Purpose of ginkgolic acid in preparing external preparation for treating venereal disease, gynopathy and perianal disease | |
| CN106880654B (en) | Application of panax japonicus extract in preparing medicine for treating rhinitis and composition | |
| CN103751105A (en) | Application of ginkgolic acid nanometer liposome in treatment of gynecological diseases and venereal diseases | |
| CN103751230A (en) | Application of gingko extract nanometer liposome in gynecological disease treatment | |
| CN113440590B (en) | External toad stamen ointment for treating cancer pain | |
| CN109470788A (en) | A kind of method of quality control of FUKE QIANJIN PIAN | |
| CN111615384A (en) | Method for treating sinusitis | |
| CN103720943B (en) | A kind of Chinese medicine for the treatment of gynecological bleeding and preparation method thereof | |
| CN103735494B (en) | External antipruritic ointment for treating mosquito bite and preparation method of external antipruritic ointment | |
| WO2023020178A1 (en) | Use of cell-free fat extract for treating vaginal atrophy | |
| US20240009250A1 (en) | Therapeutic application of cell-free fat extract to arthritis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200124 |