CN110638910B

CN110638910B - A breath freshener with antiviral, antibacterial and antiinflammatory effects, and its preparation method

Info

Publication number: CN110638910B
Application number: CN201910791806.XA
Authority: CN
Inventors: 张义淦; 吴海强; 陈嘉敏
Original assignee: Baiyinxiang Biotechnology Co ltd
Current assignee: Baiyinxiang Biotechnology Co ltd
Priority date: 2019-08-26
Filing date: 2019-08-26
Publication date: 2021-07-20
Anticipated expiration: 2039-08-26
Also published as: CN110638910A

Abstract

The invention belongs to the technical field of breath fresheners, and particularly relates to a breath freshener with antiviral, antibacterial and anti-inflammatory effects and a preparation method thereof. The breath freshener provided by the invention is prepared from traditional Chinese medicine extracting solution, chitosan/nano-silver sol, OP-10 emulsifier and normal saline, wherein the traditional Chinese medicine extracting solution is prepared from the following raw materials: the oral cavity refreshing agent is prepared from dandelion, astragalus, isatis root, silvery, aloe, dyers woad leaf, forsythia, mint, propolis and other materials, the preparation method is simple, and the prepared oral cavity refreshing agent has good antiviral, antibacterial and anti-inflammatory effects.

Description

A breath freshener with antiviral, antibacterial and antiinflammatory effects, and its preparation method

Technical Field

The invention belongs to the technical field of breath fresheners, and particularly relates to a breath freshener with antiviral, antibacterial and anti-inflammatory effects and a preparation method thereof.

Background

In common words: the oral cavity is an inlet of a respiratory digestive tract, has important physiological functions of chewing, swallowing, speech expression and the like, is one of important ways for pathogenic microorganisms to invade an organism, has proper temperature and humidity in the oral cavity, and is the earliest contact with flora (bacteria and fungi) from the air, thereby providing excellent conditions for the growth and the propagation of the microorganisms. When the immunity of the organism is low, endogenous or exogenous infection is easy to occur, and the importance of oral care is particularly obvious. Oral health is an important component of whole body health, and the world health organization proposed that oral diseases are a serious public health problem in 2007, and active prevention and treatment are required. Oral health directly or indirectly affects general health. Oral diseases such as caries and periodontal disease destroy tooth hard tissue and supporting tissue around teeth, and not only affect chewing, speech and beauty, but also cause social interaction difficulty and psychological disorder. Some microorganisms exist in the oral cavity for a long time, can cause or aggravate certain systemic diseases such as coronary heart disease, diabetes and the like, harm the health of the whole body and influence the quality of life.

Oral care products are a series of products that people use to maintain oral hygiene and improve oral health, and mainly include toothpaste, toothbrushes, mouthwash, breath fresheners, dental floss, floss bars, tongue scrapers, and the like. The most efficient and convenient method is to use a breath freshener, which is a common daily necessity and is used for cleaning the oral cavity, whitening the teeth or preventing oral diseases. Although the breath freshener is convenient and fast, the breath cannot be fundamentally treated, the breath is easily regenerated, the breath is kept in the oral cavity for a short time, and the breath freshener cannot play a greater effect.

Compared with the prior art, the quality of the oral care product is greatly improved, but the oral care product still has the defects. Taking toothpaste as an example, the current toothpaste products have not yet met all the needs of consumers. For example, the claimed safer baking soda toothpaste has the disadvantage of insufficient cleaning, since baking soda toothpaste requires high concentration of baking soda ingredient to provide sufficient cleaning power, but too high concentration can affect product mouthfeel; for another example, the anti-caries toothpaste needs to be added with fluoride, but excessive fluoride intake can cause the teeth to become yellow and even cause dental fluorosis; various chemical antibacterial agents are often added into toothpaste with the anti-inflammatory and bacteriostatic effects, and although the toothpaste can play a role in efficacy, the safety of the toothpaste is worried. As the market of Chinese oral care products gradually matures, the function division of the products is more and more refined, which requires that the oral care products in the future not only have remarkable functions, but also the functional components must be safe in source, and the efficacy and the safety are both obtained.

Chinese patent application CN104274375A discloses a breath freshener for wound healing, comprising a solvent and normal saline, wherein the solvent comprises the following main components by weight: 5-15 parts of sea buckthorn, 10-20 parts of lemon, 15-22 parts of mint and 25-30 parts of dandelion; the mint is picked up fresh, cleaned, dried, rolled and extracted to obtain mint juice; selecting fresh dandelion, mashing and taking juice; selecting the dandelion after drying, and mixing the dandelion according to the weight ratio of 1: 4-1: 8, decocting with water for 20-30 min; the ratio range of the solvent to the normal saline is 1:10-1: 20; chinese patent application CN104306456A discloses a breath freshener for improving the onset of warm diseases, which consists of a solvent and normal saline, wherein the solvent mainly comprises the following components in parts by weight: 5-15 parts of burdock, 5-15 parts of fructus forsythiae, 10-20 parts of honeysuckle, 15-22 parts of fennel seed and 25-30 parts of mint; collecting fruits of fructus Arctii, removing impurities, sun drying, decocting, and collecting juice; the fennel seeds need to be extracted with essential oil for use; selecting fresh leaves of the mint, cleaning, smashing into juice, and extracting clean juice; the proportion range of the solvent and the normal saline is as follows: 1:10-1: 20; the breath freshener prepared by the two methods can effectively relieve cough and remove oral peculiar smell, but the antibacterial and anti-inflammatory effects are still to be improved.

Therefore, how to develop and obtain a functional breath freshening spray which has wide sterilization and virus resistance range, can directly act on bacteria and viruses in the air and has obvious and stable effect is a difficult problem which needs to be solved in the field urgently.

Disclosure of Invention

In order to overcome the technical problems, the invention provides the breath freshener with the functions of resisting virus, inhibiting bacteria and diminishing inflammation, the preparation method is simple, and the prepared breath freshener has better functions of resisting virus, inhibiting bacteria and diminishing inflammation, and can be retained in the oral cavity for a long time, so that the effective components in the breath freshener can better exert the effect.

In order to achieve the above purpose, the technical scheme provided by the invention is as follows:

a breath freshener with antiviral, antibacterial and anti-inflammatory effects comprises the following components:

traditional Chinese medicine extracting solution, chitosan/nano silver sol and OP-10 emulsifier;

wherein the traditional Chinese medicine extracting solution is prepared from the following raw materials: dandelion, astragalus root, isatis root, white silver incense, aloe, isatis leaf, forsythia fruit, peppermint and propolis.

Preferably, the breath freshener comprises the following components in percentage by mass: 5-15% of traditional Chinese medicine extracting solution, 5-15% of chitosan/nano silver sol, 0.1-0.5% of OP-10 emulsifier and the balance of normal saline;

preferably, the breath freshener comprises the following components in percentage by mass: 5-10% of traditional Chinese medicine extracting solution, 5-8% of chitosan/nano silver sol, 0.2-0.4% of OP-10 emulsifier and the balance of normal saline;

preferably, the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 180 portions of dandelion, 250 portions of astragalus root, 160 portions of isatis root, 80-150 portions of silver incense, 90-150 portions of aloe, 70-180 portions of dyers woad leaf, 80-170 portions of forsythia, 60-130 portions of peppermint and 80-160 portions of propolis.

Preferably, the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 200 portions of dandelion, 180 portions of astragalus, 200 portions of isatis root, 220 portions of isatis root, 90-120 portions of silver incense, 100 portions of aloe, 150 portions of dyers woad leaf, 100 portions of forsythia, 80-110 portions of mint and 120 portions of propolis.

Preferably, the nano silver has a particle size of 10 to 80 nm.

Preferably, the preparation method of the chitosan/nano silver sol comprises the following steps: preparing acetic acid-sodium acetate buffer solution, preparing chitosan solution with the buffer solution, adding silver nitrate solution, stirring, and adding punicalagin.

Preferably, the preparation method of the chitosan/nano silver sol comprises the following steps: preparing acetic acid-sodium acetate buffer solution with the pH value of 3-7, preparing chitosan solution with the mass fraction of 0.1% -1.5% by using the buffer solution, taking 50-150ml of the prepared chitosan solution, adding 300ml of 0.1-1.0mol/L silver nitrate solution, stirring for 0.5-1h, adding 1-3ml of 0.2% -1.5% punicalagin, and stirring for 2-8h at room temperature.

Preferably, the preparation method of the chitosan/nano silver sol comprises the following steps: preparing acetic acid-sodium acetate buffer solution with the pH value of 3-7, preparing chitosan solution with the mass fraction of 0.1% -1.5% by using the buffer solution, taking 100ml of the prepared chitosan solution, adding 250ml of 0.1-1.0mol/L silver nitrate solution, stirring for 0.5-1h, adding 1-3ml of 0.2% -1.5% punicalagin, and stirring for 2-8h at room temperature.

The invention also aims to provide a preparation method of the breath freshener with antiviral, antibacterial and anti-inflammatory effects, which comprises the following steps:

s1, crushing the traditional Chinese medicine raw materials to obtain traditional Chinese medicine particles; adding the traditional Chinese medicine granules into normal saline, soaking, boiling, filtering and carrying out ultrasound to obtain a traditional Chinese medicine extracting solution;

s2, weighing the traditional Chinese medicine extracting solution, the chitosan/nano silver sol and the OP-10 emulsifier, and uniformly mixing to obtain a mixed solution 1;

s3, adding physiological saline into the mixed solution 1, and stirring to obtain the antiviral, antibacterial and anti-inflammatory breath freshener.

Preferably, in S1, the mass ratio of the physiological saline to the traditional Chinese medicine particles is 80-70: 20-30;

preferably, in S1, the soaking time is 3-4 h;

preferably, in S1, the boiling time is 1-2 h;

preferably, in S1, the ultrasonic time is 30-60 min;

preferably, in S1, the power of the ultrasound is 90-150W;

preferably, in S1, the filtering is performed with a filter screen of 300 meshes and 400 meshes;

preferably, in S2, the blending specifically comprises: stirring for 1-2h at 5000-;

preferably, in S3, the rotation speed of the stirring is 5000-.

Compared with the prior art, the invention has the technical advantages that:

(1) the prepared breath freshener contains medicinal plant extract components and functional components for protecting mucosa, can prevent mass propagation of malodorous bacteria, has the effects of bacteriostasis, inflammation diminishing, hemostasis, deodorization and the like, and also has the effect of antivirus.

(2) The breath freshener has strong fragrance, can freshen breath, moisten oral cavity, can inhibit and fade halitosis to a certain extent, can cover up mild halitosis breath, and can be used for refreshing breath; is suitable for not only being used in the mouth in an untimely way, but also being used for daily oral care.

(3) The prepared breath freshener has the effects of cleaning oral cavities and teeth to a certain extent, relieving uncomfortable symptoms such as toothache, gingival bleeding, oral ulcer, gingivitis, periodontitis, sore throat and the like, is obviously helpful for preventing and treating cold, oral ulcer, periodontitis and gingivitis, and can protect oral health.

(4) The traditional Chinese medicine extract is compounded with the nano-silver material, and the traditional Chinese medicine extract is positioned outside the particles through adsorption action, adhesion action or dissolution and wrapping action, so that the effective components of the breath freshener have longer detention time in the oral cavity, have obvious antiviral, antibacterial and anti-inflammatory effects, can be used for a long time and have no toxic or side effect.

Detailed Description

Example 1

In the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 10% of traditional Chinese medicine extracting solution, 8% of chitosan/nano-silver sol, 0.4% of OP-10 emulsifier and 81.6% of normal saline;

the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 200 parts of dandelion, 180 parts of astragalus, 200 parts of isatis root, 90 parts of silver incense, 100 parts of aloe, 120 parts of folium isatidis, 100 parts of fructus forsythiae, 80 parts of mint and 100 parts of propolis;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 5, preparing 0.1% chitosan solution by mass fraction by using the buffer solution, measuring 100ml, adding 250ml of 0.1mol/L silver nitrate solution, magnetically stirring for 0.5h, adding 2ml of 0.2% punicalagin, and continuously stirring for 6h at room temperature to obtain chitosan/nano silver sol; wherein the average nano-particle size is 10 nm;

(2) preparing the breath freshener with antiviral, antibacterial and anti-inflammatory effects:

s1, crushing the traditional Chinese medicine raw materials to obtain traditional Chinese medicine particles; adding the Chinese medicinal granules into normal saline, soaking for 3h, boiling for 1h, filtering, and performing ultrasonic treatment at 150W for 30min to obtain Chinese medicinal extractive solution; wherein the mass ratio of the normal saline to the traditional Chinese medicine particles is 75: 25;

s2, weighing 10% of traditional Chinese medicine extracting solution, 8% of chitosan/nano-silver sol and 0.4% of OP-10 emulsifier according to the formula of the breath freshener, and stirring for 1h at 5000r/min to be uniformly mixed to obtain a mixed solution 1 of the traditional Chinese medicine extracting solution and the chitosan/nano-silver sol;

s3, adding 81.6% of normal saline into the mixed solution 1, and stirring for 16 hours at the rotating speed of 5000r/min by using a stirrer to obtain the antiviral, antibacterial and anti-inflammatory breath freshener.

Example 2

In the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 5% of traditional Chinese medicine extracting solution, 10% of chitosan/nano-silver sol, 0.5% of OP-10 emulsifier and 84.5% of normal saline;

the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 180 parts of dandelion, 160 parts of astragalus, 160 parts of isatis root, 80 parts of silver incense, 90 parts of aloe, 70 parts of folium isatidis, 80 parts of fructus forsythiae, 60 parts of mint and 80 parts of propolis;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 3, preparing 1.5% chitosan solution by mass fraction by using the buffer solution, measuring 50ml, adding 200ml of 1.0mol/L silver nitrate solution, magnetically stirring for 0.5h, adding 1ml of 1.5% punicalagin, and continuously stirring for 2h at room temperature to obtain chitosan/nano silver sol; wherein the average nano-particle size is 80 nm;

s1, crushing the traditional Chinese medicine raw materials to obtain traditional Chinese medicine particles; adding the Chinese medicinal granules into normal saline, soaking for 4 hr, boiling for 2 hr, filtering, and performing ultrasonic treatment under 90W for 60min to obtain Chinese medicinal extractive solution; wherein the mass ratio of the normal saline to the traditional Chinese medicine particles is 80: 20;

s2, weighing 5% of traditional Chinese medicine extracting solution, 15% of chitosan/nano-silver sol and 0.5% of OP-10 emulsifier according to the formula of the breath freshener, and stirring for 2 hours at 5000r/min to be uniformly mixed to obtain a mixed solution 1 of the traditional Chinese medicine extracting solution and the chitosan/nano-silver sol;

s3, adding 79.5% of normal saline into the mixed solution 1, and stirring for 24 hours at the rotating speed of 6000r/min by using a stirrer to obtain the antiviral, antibacterial and anti-inflammatory breath freshener.

Example 3

In the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 15% of traditional Chinese medicine extracting solution, 5% of chitosan/nano-silver sol, 0.1% of OP-10 emulsifier and 79.9% of normal saline;

the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 250 parts of dandelion, 200 parts of astragalus, 220 parts of isatis root, 120 parts of silver incense, 120 parts of aloe, 150 parts of folium isatidis, 150 parts of fructus forsythiae, 110 parts of mint and 120 parts of propolis;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 7, preparing 0.3% chitosan solution by using the buffer solution, measuring 150ml, adding 300ml of 0.3mol/L silver nitrate solution, adding 3ml of 0.5% punicalagin after magnetically stirring for 1h, and continuously stirring for 8h at room temperature to obtain chitosan/nano silver sol 1; wherein the average nano-particle size is 30 nm;

s1, crushing the traditional Chinese medicine raw materials to obtain traditional Chinese medicine particles; adding the Chinese medicinal granules into normal saline, soaking for 4h, boiling for 2h, filtering, and performing ultrasonic treatment at 110W for 40min to obtain Chinese medicinal extractive solution; wherein the mass ratio of the normal saline to the traditional Chinese medicine particles is 70: 30;

s2, weighing 15% of traditional Chinese medicine extracting solution, 5% of chitosan/nano-silver sol and 0.1% of OP-10 emulsifier according to the formula of the breath freshener, and stirring for 2 hours at 6000r/min to uniformly mix to obtain a mixed solution 1 of the traditional Chinese medicine extracting solution and the chitosan/nano-silver sol;

s3, adding 79.9% of normal saline into the mixed solution 1, and stirring for 12 hours at the rotating speed of 6000r/min by using a stirrer to obtain the antiviral, antibacterial and anti-inflammatory breath freshener.

Example 4

In the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 5% of traditional Chinese medicine extracting solution, 3% of chitosan/nano silver sol, 0.2% of OP-10 emulsifier and 91.8% of normal saline;

the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 270 parts of dandelion, 250 parts of astragalus, 250 parts of isatis root, 150 parts of silver incense, 150 parts of aloe, 180 parts of folium isatidis, 170 parts of fructus forsythiae, 130 parts of mint and 160 parts of propolis;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 6, preparing 1.0% chitosan solution by using the buffer solution, measuring 100ml, adding 250ml of 0.8mol/L silver nitrate solution, adding 3ml of 1.2% punicalagin after magnetically stirring for 1h, and continuously stirring for 5h at room temperature to obtain chitosan/nano silver sol; wherein the average nano-particle size is 60 nm;

s1, crushing the traditional Chinese medicine raw materials to obtain traditional Chinese medicine particles; adding the Chinese medicinal granules into normal saline, soaking for 3h, boiling for 1h, filtering, and performing ultrasonic treatment at 130W for 30min to obtain Chinese medicinal extractive solution; wherein the mass ratio of the normal saline to the traditional Chinese medicine particles is 75: 25;

s2, weighing 5% of traditional Chinese medicine extracting solution, 5% of chitosan/nano-silver sol and 0.2% of OP-10 emulsifier according to the formula of the breath freshener, and stirring for 1h at 6000r/min to be uniformly mixed to obtain a mixed solution 1 of the traditional Chinese medicine extracting solution and the chitosan/nano-silver sol;

s3, adding 89.8% of normal saline into the mixed solution 1, and stirring for 24 hours at the rotating speed of 5000r/min by using a stirrer to obtain the antiviral, antibacterial and anti-inflammatory breath freshener.

Comparative example 1

Compared with the example 1, the preparation of the chitosan/nano silver sol is different;

in this example, the composition of the breath freshener with antiviral, antibacterial and anti-inflammatory effects is the same as that of example 1;

the preparation raw materials of the traditional Chinese medicine extracting solution are the same as those in the example 1;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 5, preparing 0.1% chitosan solution by mass percent by using the buffer solution, measuring 100ml, adding 250ml of 0.1mol/L silver nitrate solution, magnetically stirring for 0.5h, adding 2g of sodium borohydride, and continuously stirring for 6h at room temperature to obtain chitosan/nano-silver sol; wherein the average nano-particle size is 10 nm;

(2) the method for preparing the breath freshener with antiviral, antibacterial and anti-inflammatory effects is the same as that of example 1.

Comparative example 2

Compared with example 1, the Chinese medicinal extractive solution is prepared from different raw materials.

the preparation raw materials of the traditional Chinese medicine extracting solution comprise: 200 parts of dandelion, 180 parts of astragalus, 200 parts of isatis root, 90 parts of sea-buckthorn, 100 parts of aloe, 120 parts of folium isatidis, 100 parts of fructus forsythiae, 80 parts of mint and 100 parts of propolis;

(1) the preparation of the chitosan/nano silver sol was the same as example 1:

Comparative example 3

The composition of the breath freshener was different from that of example 1;

in the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 3% of traditional Chinese medicine extracting solution, 15% of chitosan/nano-silver sol, 0.4% of OP-10 emulsifier and 81.6% of normal saline;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 5, preparing 0.1% chitosan solution by using the buffer solution, measuring 100ml, adding 250ml of 0.1mol/L silver nitrate solution, adding 2ml of 1% punicalagin after magnetic stirring for 0.5h, and continuously stirring for 6h at room temperature to obtain chitosan/nano silver sol; wherein the average nano-particle size is 10 nm;

Comparative example 4

The composition of the breath freshener was different from that of example 1;

in the embodiment, the breath freshener with the antiviral, antibacterial and anti-inflammatory effects comprises the following components: 20% of traditional Chinese medicine extracting solution, 1% of chitosan/nano-silver sol, 0.4% of OP-10 emulsifier and 78.6% of normal saline;

(1) preparing chitosan/nano silver sol:

preparing acetic acid-sodium acetate buffer solution with the pH value of 5, preparing 1% chitosan solution by mass percent by using the buffer solution, measuring 100ml, adding 250ml of 0.5mol/L silver nitrate solution, magnetically stirring for 0.5h, adding 2ml of 1% punicalagin, and continuously stirring for 6h at room temperature to obtain chitosan/nano-silver sol; wherein the average nano-particle size is 10 nm;

Effect test

The breath fresheners prepared in examples 1 to 4 of the present invention and comparative examples 1 to 4 were subjected to a bactericidal antiviral test by a method of disinfection technical code 2017 of ministry of health, and the specific results are shown in tables 1 and 2 below.

TABLE 1 antiviral Effect of the freshener (directly sprayed in the air)

TABLE 2 bactericidal Effect of breath fresheners

Group of	Pneumoniae	Staphylococcus aureus	Escherichia coli	Pseudomonas aeruginosa
					Example 1	97％	98％	99％	95％
Example 2	93％	93％	94％	93％
					Example 3	95％	94％	97％	97％
Example 4	96％	95％	98％	96％
					Comparative example 1	81％	83％	78％	80％
Comparative example 2	79％	80％	82％	77％
					Comparative example 3	86％	85％	89％	85％
Comparative example 4	83％	82％	93％	83％

Therefore, the breath freshener provided by the invention has better antiviral and antibacterial effects, and the composition and formula dosage of the raw materials, the chitosan/nano silver sol and the like have important influence on the effect in the preparation process.

Anti-inflammatory test

100 Kunming mice with the weight of 200 plus or minus 20 g; randomly divided into 10 groups, namely a model group, a blank control group (physiological saline), examples 1-4 and comparative examples 1-4; spraying for administration, 1 time per day for 5 days, smearing 50 μ L of xylene on front and back surfaces of left ear of mouse 1h after last administration, taking right ear as control, removing neck of mouse after 2h, killing, cutting two ears along auricle base line, punching round ear piece at the same position with 9mm puncher, weighing, and taking difference between left and right ear pieces as swelling degree; the results of the experiment are shown in table 3 below:

TABLE 3 inhibition effect data sheet

Remarking: the different letters of a, b and c represent significant differences (p < 0.05);

the result shows that the breath freshener prepared by the invention has better anti-inflammatory effect.

The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.

Claims

1. A breath freshener with antiviral, antibacterial and anti-inflammatory effects is prepared from the following components:

5-15% of traditional Chinese medicine extracting solution, 3-10% of chitosan/nano silver sol, 0.1-0.5% of OP-10 emulsifier and the balance of normal saline;

the traditional Chinese medicine extracting solution is prepared from the following raw materials: 180 portions of dandelion, 250 portions of astragalus root, 250 portions of isatis root, 80-150 portions of silver incense, 90-150 portions of aloe, 70-180 portions of dyers woad leaf, 80-170 portions of forsythia, 60-130 portions of mint and 80-160 portions of propolis;

the preparation method of the chitosan/nano silver sol comprises the following steps: preparing acetic acid-sodium acetate buffer solution, preparing chitosan solution with the buffer solution, adding silver nitrate solution, stirring, and adding punicalagin.

2. The breath freshener according to claim 1, wherein the traditional Chinese medicine extracting solution is prepared from the following raw materials: 200 portions of dandelion, 180 portions of astragalus, 200 portions of isatis root, 220 portions of isatis root, 90-120 portions of silver incense, 100 portions of aloe, 150 portions of dyers woad leaf, 100 portions of forsythia, 80-110 portions of mint and 120 portions of propolis.

3. The breath freshener according to claim 1, wherein the nano silver particle size in the chitosan/nano silver sol is 10 to 80 nm.

4. A method of preparing a breath freshener according to any one of claims 1 to 3, comprising the steps of:

5. The method of claim 4, wherein in S1, the mass ratio of the normal saline to the traditional Chinese medicine particles is (80-70): (20-30).

6. The method of preparing a breath freshener according to claim 4, wherein the soaking time is 3 to 4 hours in S1; the boiling time is 1-2 h; the power of the ultrasonic wave is 90-150W; the ultrasonic treatment time is 30-60 min.

7. The method for preparing the breath freshener according to claim 4, wherein the rotation speed of the stirring is 5000-.