CN110585265B - 一种马拉色菌抑制剂 - Google Patents
一种马拉色菌抑制剂 Download PDFInfo
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- 241000555676 Malassezia Species 0.000 title claims abstract description 16
- 239000003112 inhibitor Substances 0.000 title abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 15
- 235000005116 Stachys sieboldii Nutrition 0.000 claims abstract description 13
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 10
- 239000000126 substance Substances 0.000 claims abstract description 5
- 241000424764 Stachys affinis Species 0.000 claims abstract 3
- 239000003814 drug Substances 0.000 claims description 7
- 229940079593 drug Drugs 0.000 claims description 6
- 239000000469 ethanolic extract Substances 0.000 claims description 4
- 208000001840 Dandruff Diseases 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 206010039793 Seborrhoeic dermatitis Diseases 0.000 abstract description 4
- 208000008742 seborrheic dermatitis Diseases 0.000 abstract description 4
- 206010016936 Folliculitis Diseases 0.000 abstract description 3
- 208000007712 Tinea Versicolor Diseases 0.000 abstract description 3
- 206010056131 Tinea versicolour Diseases 0.000 abstract description 3
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 3
- 239000002537 cosmetic Substances 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract description 2
- 208000017520 skin disease Diseases 0.000 abstract description 2
- 244000057214 Stachys sieboldii Species 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 238000001035 drying Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 229940043810 zinc pyrithione Drugs 0.000 description 3
- PICXIOQBANWBIZ-UHFFFAOYSA-N zinc;1-oxidopyridine-2-thione Chemical compound [Zn+2].[O-]N1C=CC=CC1=S.[O-]N1C=CC=CC1=S PICXIOQBANWBIZ-UHFFFAOYSA-N 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 210000004761 scalp Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XMAYWYJOQHXEEK-OZXSUGGESA-N (2R,4S)-ketoconazole Chemical compound C1CN(C(=O)C)CCN1C(C=C1)=CC=C1OC[C@@H]1O[C@@](CN2C=NC=C2)(C=2C(=CC(Cl)=CC=2)Cl)OC1 XMAYWYJOQHXEEK-OZXSUGGESA-N 0.000 description 1
- OWEGWHBOCFMBLP-UHFFFAOYSA-N 1-(4-chlorophenoxy)-1-(1H-imidazol-1-yl)-3,3-dimethylbutan-2-one Chemical compound C1=CN=CN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 OWEGWHBOCFMBLP-UHFFFAOYSA-N 0.000 description 1
- 241001083548 Anemone Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
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- 206010059866 Drug resistance Diseases 0.000 description 1
- 244000119298 Emblica officinalis Species 0.000 description 1
- 235000015489 Emblica officinalis Nutrition 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 241000555688 Malassezia furfur Species 0.000 description 1
- 241001571466 Potentilla discolor Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000519999 Stachys Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229960003344 climbazole Drugs 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 229960004125 ketoconazole Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000009240 nasopharyngitis Diseases 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
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Abstract
本发明公开了一种马拉色菌抑制剂，体外抑菌试验结果表明，草石蚕提取物对马拉色菌具有明显的抑制效果，是一种优良的马拉色菌抑制剂，可用于治疗花斑癣、毛囊炎、脂溢性皮炎等皮肤疾病，也可被开发成抗头皮屑的日化用品或化妆品，具有广泛的应用潜力，由于来源于天然植物，因此也具有很高的安全性。
Description
技术领域
本发明涉及一种马拉色菌抑制剂。
背景技术
草石蚕(Stachys sieboldii Miq.)为唇形科、水苏属、多年生草本植物，因其地下茎形似蚕蛹或螺丝，因此又称螺丝菜、宝塔菜、甘露子、地蚕等。草石蚕药用部位为根茎，在《本草纲目》、《陆川本草》中均有记载，它味甘，性平，具有解表清肺、祛风除湿、清热解毒等功效，常用于风热感冒、虚劳咳嗽、黄疸、淋证、疮毒肿痛等治疗。
马拉色菌是皮肤表面的常驻菌，也是花斑癣、毛囊炎的病原菌，与脂溢性皮炎等发病有关，目前已有大量的科研成果证实马拉色菌在头皮的过度增生是导致头皮脂溢性皮炎(头皮屑)的重要原因。因此，在抗头皮屑的日化用品和化妆品中，人们将目光主要集中在抗马拉色菌活性药物的研发，如酮康唑、氯咪巴唑、吡硫鎓锌是公认有效的几种抗马拉色菌和治疗头皮屑药物，但是这些药物刺激性较大，长期使用还会产生耐药性。目前，人们已经发现了许多天然植物具有抗真菌活性，与化学药物相比，这些天然植物具有更好的安全性。
发明内容
本发明的目的是提供一种草石蚕提取物抑制马拉色菌的新用途，以及提供一种马拉色菌抑制剂。
体外抑菌试验结果表明：草石蚕提取物对马拉色菌具有明显的抑制效果，其中醇提取物的抑制作用大于水提取物，70％乙醇提取物的抑制作用最强。
以上结果表明：草石蚕提取物是一种优良的马拉色菌抑制剂，可用于治疗花斑癣、毛囊炎、脂溢性皮炎等皮肤疾病，也可被开发成抗头皮屑的日化用品或化妆品，具有广泛的应用潜力，由于来源于天然植物，因此也具有很高的安全性。
具体实施方式
下面通过实施例对本发明进行详细地说明。
实施例1
取草石蚕，加水煎煮提取两次，每次2小时，收集提取液，浓缩后干燥，粉碎成细粉，得到草石蚕水提取物。
实施例2
取草石蚕，分别用体积浓度为50％、70％、90％的乙醇回流提取两次，每次2小时，收集提取液，浓缩后干燥，粉碎成细粉，得到三种草石蚕醇提取物。
实施例3
1)将草石蚕水提取物、醇提取物分别用无菌水溶解，制成固含量为0.05g/ml的待测药液；
2)将购买得到的糠秕马拉色菌进行活化培养，用无菌水配置成浓度为107cfu/ml的菌悬液；
3)将橄榄油培养基倾注于平板，冷却凝固后取马拉色菌菌悬液500μl于培养基中，均匀涂布后干燥15min；
4)在培养皿上方摆放3个牛津杯，3个牛津杯中注入同一种待测药液，作为三个平行，以无菌水为阴性对照，吡硫鎓锌(ZPT)为阳性对照。
5)将培养皿放入37℃培养箱恒温培养48小时，然后取出，观察是否出现抑菌圈并用游标卡尺测量直径。
结果发现，阴性对照无抑菌圈形成，4种待测药液和阳性对照均有抑菌圈形成，抑菌圈直径见下表。
表1草石蚕提取物对马拉色菌的抑制效果
注：以上结果均为三个平行的平均值。
以上结果表明：草石蚕提取物均对马拉色菌具有明显的抑制效果，其中醇提取物的抑制作用大于水提取物，70％醇提取物的抑制活性最强。
Claims (1)
1.草石蚕提取物在制备抑制马拉色菌的药物或日化用品中的用途，所述草石蚕提取物是草石蚕70％的乙醇提取物。
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| CN114010677B (zh) * | 2021-12-27 | 2023-06-23 | 武汉禾本泰医药科技有限公司 | 一种治疗花斑癣的药物组合物 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101419198A (zh) * | 2007-10-22 | 2009-04-29 | 重庆新禹投资（集团）有限公司 | 水苏糖含量快速检测技术 |
| CN106237374A (zh) * | 2016-08-17 | 2016-12-21 | 林春梅 | 一种医用抗菌水凝胶敷料及其制备方法 |
| CN106668850A (zh) * | 2015-11-05 | 2017-05-17 | 惠众国际医疗器械（北京）有限公司 | 一组天然杀菌/抗菌剂 |
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| JP2010529175A (ja) * | 2007-06-14 | 2010-08-26 | インスティトゥート・バイオマール・ソシエダード・アノニマ | マラセチア属酵母に対して抗真菌活性を有するテルペン |
| CN102743363B (zh) * | 2011-04-20 | 2015-01-21 | 上海邦氏生物科技有限公司 | 和厚朴酚及其衍生物制备治疗马拉色菌引起皮肤疾病的制品的用途 |
| CN102218048B (zh) * | 2011-04-21 | 2013-02-27 | 上海莱博生物科技有限公司 | 厚朴酚在制备治疗马拉色菌引起皮肤病的外用制剂和日化品中应用 |
| KR101302678B1 (ko) * | 2012-09-18 | 2013-09-09 | 전라남도 | 동백나무 추출물을 유효성분으로 포함하는 항진균용 조성물 |
| JP6826883B2 (ja) * | 2016-12-26 | 2021-02-10 | ライオン株式会社 | Ｍａｌａｓｓｅｚｉａ属真菌に対する増殖抑制剤、Ｍａｌａｓｓｅｚｉａ属真菌性皮膚疾患の予防乃至治療剤、及びＭａｌａｓｓｅｚｉａ属真菌の除菌、除去乃至増殖抑制方法 |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101419198A (zh) * | 2007-10-22 | 2009-04-29 | 重庆新禹投资（集团）有限公司 | 水苏糖含量快速检测技术 |
| CN106668850A (zh) * | 2015-11-05 | 2017-05-17 | 惠众国际医疗器械（北京）有限公司 | 一组天然杀菌/抗菌剂 |
| CN106237374A (zh) * | 2016-08-17 | 2016-12-21 | 林春梅 | 一种医用抗菌水凝胶敷料及其制备方法 |
Non-Patent Citations (2)
| Title |
|---|
| 水苏糖热量低且不引发龋齿;中国食品报新闻;《搜狐》;20181221;第1页第2段 * |
| 草石蚕多糖的提取及其性质研究;白瑞;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20150315;"摘要" * |
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